US20070017914A1 - Porous film cartridge - Google Patents

Porous film cartridge Download PDF

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Publication number
US20070017914A1
US20070017914A1 US10/575,366 US57536606A US2007017914A1 US 20070017914 A1 US20070017914 A1 US 20070017914A1 US 57536606 A US57536606 A US 57536606A US 2007017914 A1 US2007017914 A1 US 2007017914A1
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United States
Prior art keywords
porous membrane
barrel
cap
opening edge
sandwiching
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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US10/575,366
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English (en)
Inventor
Keiji Shigesada
Morio Fujiwara
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Fujifilm Holdings Corp
Fujifilm Corp
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Individual
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Filing date
Publication date
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Assigned to FUJI PHOTO FILM CO., LTD. reassignment FUJI PHOTO FILM CO., LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FUJIWARA, MORIO, SHIGESADA, KEIJI
Publication of US20070017914A1 publication Critical patent/US20070017914A1/en
Assigned to FUJIFILM CORPORATION reassignment FUJIFILM CORPORATION ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FUJIFILM HOLDINGS CORPORATION (FORMERLY FUJI PHOTO FILM CO., LTD.)
Priority to US12/891,293 priority Critical patent/US20110072636A1/en
Abandoned legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
    • B01D29/012—Making filtering elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/085—Funnel filters; Holders therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/147—Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/081—Manufacturing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
    • B01D65/003—Membrane bonding or sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/02—Specific tightening or locking mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/04—Specific sealing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/04—Specific sealing means
    • B01D2313/042—Adhesives or glues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/20—Specific housing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T29/00—Metal working
    • Y10T29/49—Method of mechanical manufacture
    • Y10T29/49826—Assembling or joining
    • Y10T29/49908—Joining by deforming

Definitions

  • the present invention relates to a porous membrane cartridge used for a filtration of a liquid and the like.
  • a porous membrane is widely used in a laboratory and a factory for a filtration of a liquid and an adsorption of a specific substance in the liquid. Then in using the porous membrane for such a purpose, it is requested to hold the porous membrane on the way of a passage where the liquid passes.
  • the holding method is generally used a method of holding the porous membrane with sandwiching it between two members having a passage where the liquid passes.
  • porous membrane Because such a porous membrane is generally used in a precise experiment and measurement, a purified one is requested and is usually changed if once used. Therefore, it is convenient in points of a purification and an expedience in use to make a porous membrane a cartridge holding the porous membrane in a state of being able to pass a liquid therethrough.
  • a porous membrane As such a porous membrane is known, for example, such a nucleic acid refining unit described in Japanese Patent Laid-Open Publication No. 2002-345465.
  • the nucleic acid in a case of using the conventional porous membrane in refining a nucleic acid, although the nucleic acid is adsorbed to the porous membrane, then is adsorbed to the porous membrane, and making a specific liquid flow, the nucleic acid is desorbed; however, if unable to make the liquid for desorption sufficiently flow also in corner portions of the porous membrane, there is a problem that: the nucleic acid remains in the corners and a recovery efficiency of the nucleic acid worsens.
  • the present invention is made, and a purpose thereof is to provide a porous membrane cartridge for preventing a liquid from going around a side portion thereof.
  • a porous membrane cartridge of the present invention is configured as follows: Namely, the porous membrane cartridge comprises a cylindrical barrel having openings at a top end and a rear end, respectively; a cap formed into a cylindrical shape having a fit-in portion fitted outside the top end, abutting with an opening edge of the top end, and having a sandwiching face for sandwiching a porous membrane between itself and the barrel; and the porous membrane sandwiched between the opening edge of the barrel and the cap, wherein the cap is fixed to the barrel so as not to be pulled out of the barrel in a state of crushing a peripheral edge of the porous membrane and sandwiching the porous membrane between itself and the cap.
  • an opening edge of a barrel and a sandwiching face of a cap are sandwiched in a state of a peripheral edge of the porous membrane being crushed.
  • the porous membrane has a porosity, it is strongly sandwiched by the sandwiching face between the opening edge of the barrel and the sandwiching face of the cap, the porosity is crushed as if occluded, and a liquid results in not flowing therefrom.
  • the cap is fixed to the barrel in this state, the liquid does not go around a side portion of the porous membrane. Accordingly, when using the porous membrane cartridge in filtration and adsorption, it is enabled to prevent a discharged liquid from being contaminated by a liquid going around the side portion of the porous membrane.
  • the cap to the barrel can be used any one of a fit-in, adhesion, ultrasonic welding, laser welding, insert molding, and the like.
  • the opening edge of the barrel is formed to a taper where an inner perimeter side is more retreated than an outer perimeter side
  • it is preferable that the opening edge of the barrel is welded to the sandwiching face of the cap by ultrasound.
  • welding can be performed by the portion of the outer perimeter and a sandwiching force of the porous membrane gradually becomes smaller as the porous membrane draws apart from the welded portion (namely, the portion of the outer perimeter), it is enabled to avoid a stress on the porous membrane from concentrating and to prevent a breakage thereof from occurring.
  • the taper is continuously formed into a flat portion formed at an outmost perimeter of the opening edge, it sandwiches the porous membrane by a clear welded portion (energy director), it is enabled to reduce an unnecessary positional displacement of the porous membrane.
  • a porous membrane cartridge may also be configured by: circularly forming a bead portion as a welded portion (energy director), additionally the porous membrane is sandwiched and crushed with the bead portion, and the cap and the barrel are welded by ultrasound.
  • any one of the adhesion portion and welded portion of the barrel and the cap is not limited to the opening edge and the sandwiching face.
  • the barrel may also have a joint portion for abutting with an opening edge of a fit-in portion of the cap on own outer perimeter face, make the cap and the barrel adhere or be welded between the opening edge and the joint portion, and thereby configure a porous membrane cartridge.
  • the cap in a case of fixing the cap by fitting in the barrel, it is enabled to fix the cap to the barrel by an engagement between any one of a depression and a protrusion formed on an outer perimeter face of the barrel and any one of those formed on an inner perimeter face of the cap.
  • the protrusion on the outer perimeter face of the barrel and the protrusion on the inner perimeter face of the cap may be engaged.
  • a cap molded in advance and a porous membrane disposed in the cap within a cavity of an injection molding mold, to inject a molding material in the cavity, to thereby mold the barrel, and to simultaneously sandwich a porous membrane with the opening edge of the barrel and the sandwiching face of the cap.
  • a porous membrane cartridge unit where a plurality of porous membrane cartridges are connected, it is enabled to align a sandwiching force of porous membranes of the plurality of the porous membrane cartridges, and while simultaneously forming a plurality of barrels, to make up a state of these barrels being stably connected.
  • porous membrane cartridge of the present invention is enabled to prevent a liquid from going around a side portion of the porous membrane.
  • FIG. 1 is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention.
  • FIG. 2 is a section view of a porous membrane cartridge related to the first embodiment.
  • FIG. 3 is an enlarged section perspective view of a cap related to the first embodiment.
  • FIG. 4 is an enlarged view of a part A in FIG. 2 , and shows a state before a barrel 10 and a cap 20 are welded.
  • FIG. 5 is drawings showing a process of assembling a porous membrane cartridge related to the first embodiment; (a) shows a state before welding; (b) shows a state of starting to crush a porous membrane F; (c) shows a state on the way of the welding; and (d) shows a state after the welding.
  • FIG. 6 is section views showing a porous membrane cartridge 1 A related to a second embodiment of the present invention and corresponding to the enlarged view of the part A in FIG. 2 ; (a) shows a state before welding; (b) shows a state on the way of the welding; and (c) shows a state after the welding.
  • FIG. 7 is a section view showing another example of the porous membrane cartridge 1 A related to the second embodiment and corresponding to the enlarged view of the part A in FIG. 2 .
  • FIG. 8 is drawings showing a porous membrane cartridge 1 D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A in FIG. 2 ; (a) shows a state before welding; and (b) shows a state after the welding.
  • FIG. 9 ( a ) is another example of the porous membrane cartridge 1 D of the third embodiment; and FIG. 9 ( b ) shows still another example.
  • FIG. 1 is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention
  • FIG. 2 is a section view of a porous membrane cartridge related to the first embodiment
  • FIG. 3 is an enlarged section perspective view of a cap related to the first embodiment.
  • a porous membrane cartridge 1 related to the first embodiment is configured of a porous membrane F, and a barrel 10 and a cap 20 for holding the porous membrane F and forming a passage where a liquid passes.
  • the barrel 10 comprises a cylindrical main body portion 12 and a cylindrical top end 13 continuing into the main body portion 12 , and has an opening 11 a at the top end 13 and an opening 11 b at a rear end of the main body portion 12 . Therefore, a liquid is enabled to pass from the opening 11 b to the opening 11 a .
  • An outer diameter of the top end 13 is designed to be one size smaller that of the main body portion 12 . Meanwhile, in the embodiment a side of the barrel 10 and the cap 20 in which a liquid flows is called a rear end, and a side from which the liquid is pushed out is called a top end.
  • the cap 20 comprises a cylindrical fit-in portion 22 and a nozzle 23 continuing into a top side of the fit-in portion 22 .
  • An opening 21 a is formed at the top end of the nozzle 23
  • an opening 21 b is formed at the rear end of the fit-in portion 22 , and thus a liquid is enabled to pass from the rear end to top end of the cap 20 .
  • An inner diameter of the fit-in portion 22 is formed into a diameter enabled to fit in the diameter of the top end 13 of the barrel 10 .
  • radial ribs 25 (only three are shown in FIG. 3 ) at a bottom portion 26 of the fit-in portion 22 continuing into the nozzle 23 from the portion 22 .
  • a sandwiching face 24 made higher by one step from the bottom portion 26 so as to be a same height as an upper face of the ribs 25 .
  • the sandwiching face 24 is a face for sandwiching the porous membrane F between itself and an opening edge 14 (see FIG. 1 ) corresponding to an end edge of the opening 11 a of the barrel 10 .
  • the ribs 25 are formed at the same height as the sandwiching face 24 , thereby support the porous membrane F disposed at the bottom portion 26 within the cap 20 , and prevent the porous membrane F from elongating and breaking by a liquid flowing into the top end (opening 21 a ) from the rear end (opening 21 b ).
  • the ribs 25 are radially formed, and thereby, it is designed that a liquid smoothly flows in the nozzle 23 in being made to flow from the rear end to the top end.
  • the barrel 10 and the cap 20 are composed of polystyrene, they are not limited thereto.
  • a thermoplastic resin is available that can be welded by ultrasound.
  • a material is available that can be made to adhere by adhesive.
  • the porous membrane F is composed of an organic polymer and is formed into a circle of which a diameter approximately matches the inner diameter of the cap 20 and the outer diameter of the top end 13 of the barrel 10 .
  • a material of the porous membrane F for example, a surface-saponified substance of acetylcellulose is suitable.
  • acetylcellulose although any one of mono acetylcellulose, diacetylcellulose, and triacetylcellulose is available, specifically triacetylcellulose is preferable.
  • a porous membrane of such PTFE polytetrafluoroethylene
  • polyamide polyamide
  • polypropylene polypropylene
  • polycarbonate a porous membrane of such PTFE (polytetrafluoroethylene), polyamide, polypropylene, and polycarbonate.
  • FIG. 4 is an enlarged view of a part A in FIG. 2 .
  • a slight portion (for example, a degree of 0.1 mm width) from an outmost perimeter is formed as a flat portion 14 a perpendicular to an axial line (longitudinal direction) of the barrel 10 , and continuing into the flat portion 14 a is formed a taper portion 14 b that becomes an inside taper that gradually retreats from an outer perimeter toward an inner perimeter thereof.
  • the taper portion 14 b makes a small angle of 3 to 20 degrees, for example, 10 degrees with the flat portion 14 a .
  • a diameter of an end 14 c at an inner perimeter side of the taper portion 14 b approximately matches that of an end 24 a at an inner perimeter side of the sandwiching face 24 , and from the end 14 c across the inner perimeter face 13 a of the barrel 10 , a chamfer 15 is formed that makes an angle of 60 degrees with the flat portion 14 a.
  • a longitudinal direction distance of the barrel 10 that retreats from the flat portion 14 a to the end 14 c so as to be a same degree as a thickness of the porous membrane F and so that the end 14 c abuts with a surface of the membrane F after welding the barrel 10 and the cap 20 .
  • the width of the flat portion 14 a is preferably 0.02 to 1 mm. This is because a filtration effective area decreases if the width of the flat portion 14 a is made too wide.
  • the width of the flat portion 14 a is preferably 0.02 to 0.5 mm, and more preferably 0.02 to 0.2 mm.
  • FIG. 4 and FIG. 5 showing an assembling process of the porous membrane cartridge 1 .
  • the porous membrane F As shown in FIG. 4 , dispose the porous membrane F so as to be put on the bottom portion 26 of the cap 20 . At this time, because the sandwiching face 24 and the ribs 25 are designed higher than the bottom portion 26 by one step, the porous membrane F is, to be more precise, put on the sandwiching face 24 and the ribs 25 . Then because the diameter of the porous membrane F is approximately same as the inner diameter of the cap 20 , as shown in FIG. 5 ( a ), a peripheral edge Fa of the porous membrane F results in being positioned on the sandwiching face 24 .
  • the porous membrane cartridge 1 thus made is used as follows:
  • sample solutions containing nucleic acids prepare body fluids such as a whole blood, plasma, serum, urine, human waste, semen, and saliva taken as analytes; or solutions adjusted from biotic materials such as a soluble matter and homogenate of a vegetable (or its part), an animal (or its part), and the like. Treat these solutions with a water solution containing a reagent, which solves a cell membrane and solublizes the nucleic acids. Thus the cell membrane and nucleic membrane are solved, and the nucleic acids are dispersed in the water solution.
  • body fluids such as a whole blood, plasma, serum, urine, human waste, semen, and saliva taken as analytes; or solutions adjusted from biotic materials such as a soluble matter and homogenate of a vegetable (or its part), an animal (or its part), and the like.
  • a water solution containing a reagent which solves a cell membrane and solublizes the nucleic acids.
  • a sample is a whole blood
  • red blood cells and various proteins are removed and white blood cells and nucleic membranes are solved by incubation of 10 minutes at 60 degrees Celsius in a state of addition of Guanidine Hydrochloride, Triton-X100, and Protease K (manufactured by SIGMA Corp.).
  • a sample solution is completed. Pass the sample solution while applying a pressure from the opening 11 b at the rear end side of the barrel 10 toward the opening 21 a of the top of the nozzle 23 .
  • a pressure from the opening 11 b at the rear end side of the barrel 10 toward the opening 21 a of the top of the nozzle 23 is applied.
  • the nucleic acids in the sample solution are adsorbed to the porous membrane F.
  • the nucleic acid washing buffer has a composition of desorbing not nucleic acids adsorbed to the porous membrane F but impurities, it is composed of a water solution containing a main agent and a buffer agent, and a surfactant as needed.
  • a main agent a solution containing ethanol, Tris, and Triton-X100 is preferable.
  • the porous membrane cartridge 1 of the embodiment because when passing such a sample solution where nucleic acids is dispersed, a nucleic acid washing buffer, and refined distilled water, they do not go around a side portion of the porous membrane F, it does not occur that: the acids are discharged without being adsorbed to the membrane F, and impurities mix in a solution where the acids are recovered.
  • the end 14 c is in contact with the porous membrane F and it does not also occur that the various liquids described above enter a space between the membrane F and the opening edge 14 of the barrel 10 , impurities do not mix in a nucleic acid solution after the recovery, and a nucleic acid recovery efficiency is also higher.
  • part of the opening edge 14 is formed so as to be the taper portion 14 b where an inner perimeter side is more retreated than an outer perimeter side and a sandwiching force by the opening edge 14 and the sandwiching face 24 gradually becomes smaller from an outer perimeter to an inner perimeter, it is enabled to avoid a concentration of a stress applied to the porous membrane F.
  • the opening edge 14 is designed to be a blunt angle also alleviates the concentration of the stress applied to the porous membrane F, it is enabled to prevent the membrane F from breaking.
  • the first embodiment thus described is one example of the present invention, and because it suffices that the invention may fix the barrel 10 and the cap 20 in a state of crushing and sandwiching the porous membrane F, various variation examples as below can be considered. Meanwhile, in embodiments below with respect to a same portion as in the first embodiment, a detailed description thereof will be omitted, appending a same symbol.
  • FIG. 6 is drawings showing a porous membrane cartridge 1 A related to a second embodiment of the present invention, and is section views corresponding to the enlarged view of the part A in FIG. 2 .
  • a bead 16 a of which a section is chevron is provided across a whole perimeter in the vicinity of an outer perimeter of an opening edge 16 of the barrel 10 .
  • an inner perimeter side of the bead 16 a is designed to be a flat portion 16 b for orthogonalizing an axial line of the barrel 10 .
  • the bead 16 a melts and penetrates the porous membrane F, and as shown in FIG. 6 ( b ), the sandwiching face 24 and the bead 16 a are welded. If further making the welding proceed, the flat portion 16 b crushes and sandwiches the porous membrane F between itself and the sandwiching face 24 as shown in FIG. 6 ( c ).
  • porous membrane cartridge 1 A thus made, because the opening edge 16 and the sandwiching face 24 are welded while the peripheral edge Fa of the porous membrane F melts, a liquid does not go around a side portion of the porous membrane F. In addition, because the porous membrane F is sandwiched between the flat portion 16 b and the sandwiching face 24 while crushed between them, a liquid does not enter the portion Fa of the membrane F.
  • a recovery efficiency of nucleic acids is better and mixing of impurities in a solution after a recovery is less.
  • FIG. 8 is drawings showing a porous membrane cartridge 1 D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A in FIG. 2 .
  • the porous membrane cartridge 1 D is a mode of welding the barrel 10 and the cap 20 at a portion of an outer perimeter more than the sandwiching face 24 .
  • a step portion 27 for welding defined by: a wall face portion 27 a perpendicularly rising up with respect to the sandwiching face 24 at outside of the sandwiching face 24 ; and a slant portion 27 b slantedly rising up toward the inner perimeter of the fit-in portion 22 from an upper end (namely, the rear end) in FIG. 8 ( a ) of the wall face portion 27 a.
  • a flat portion 17 a parallel with the sandwiching face 24 formed at an inner perimeter side a wall face portion 17 b composed of a peripheral face for fitting in the wall face portion 27 a formed with continuing from an outer perimeter end of the flat portion 17 a ; a slant portion 17 c formed with continuing from the top end in FIG. 8 ( a ) of the wall face portion 27 a and parallel with the slant portion 27 b ; and a flat portion 17 d formed with continuing into the slant portion 17 c and extending toward the outer perimeter of the barrel 10 .
  • porous membrane cartridge 1 D sandwiches the porous membrane F between the sandwiching face 24 and the flat portion 17 a , and the barrel 10 and the cap 20 is applied to an ultrasonic welder, the flat portion 17 d abuts with the slant portion 27 b and the abutment portion is welded. Then the peripheral edge Fa of the porous membrane F is sandwiched between the flat portion 17 a and the sandwiching face 24 in a state of being crushed.
  • FIG. 9 ( a ) it is also enabled to make the main body 12 of the barrel 10 and a face of a step difference of an outer perimeter face of the top end 13 a joint portion 18 for welding, on the other hand to form a protrusion abutting with the portion 18 across a whole perimeter at an opening edge 28 at a rear end side of the cap 20 , and to thus make a porous membrane cartridge 1 E where the joint portion 18 and the opening edge 28 are welded.
  • the barrel 10 and the cap 20 can also be made to adhere by any one of an adhesive and a UV curing resin, and the outer perimeter face of the top end 13 of the barrel 10 and the inner perimeter face of the fit-in portion 22 of the cap 20 can also be welded by laser. Meanwhile, in a case of laser welding, it is preferable to make the barrel 10 a material that can generate heat by absorbing laser or to blend a plasticizer in the barrel 10 .
  • the barrel 10 and the cap 20 As a material of the barrel 10 and the cap 20 was used polystyrene (manufactured by A&M Styrene Co., Ltd.).
  • the outer diameter of the opening edge 14 of the barrel 10 was made 7 mm
  • the width of the flat portion 14 a at outer perimeter side of opening edge 14 was made 0.05 mm
  • the angle of the taper portion 14 b was formed to be 10 degrees for the flat portion 14 a.
  • porous membrane F triacetylcellulose (Microfilter FM500 manufactured by Fuji Photo Film Co., Ltd.).
  • an ultrasonic welder 40 KHz, 800 KW manufactured by Branson Corp. as an ultrasonic welder, disposing the porous membrane F at the bottom portion 26 of the cap 20 same as in the first embodiment, and fitting the top end 13 of the barrel 10 in the cap 20 , the barrel 10 was vibrated while pressed toward the cap 20 by a horn of the ultrasonic welder. Changing a pressing force and a vibration time at this time, a welding result under each condition was determined.
  • Table 1 shows the result.
  • the symbol x indicates that the barrel 10 and the cap 20 could not be welded; the symbol ⁇ indicates that although the barrel 10 and the cap 20 could be welded, the welding was insufficient and there was a liquid leakage from the welded portion; the symbol ⁇ indicates that there was a defect that the porous membrane F was perforated or a formed product was deformed; and the symbol O indicates that the welding could be performed well without the defect.
  • the welding could not be performed in a case of the vibration time not more than 0.1 sec and could be performed well in a case of the vibration time between 0.15 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product.
  • the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product.
  • the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.35 sec; in a case of the vibration time not less than 0.4 sec, a welding time was too long and there was the defect in a formed product.
  • the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.25 sec; in a case of the vibration time not less than 0.3 sec, a welding time was too long and there was the defect in a formed product.

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  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Closures For Containers (AREA)
US10/575,366 2003-10-17 2004-09-28 Porous film cartridge Abandoned US20070017914A1 (en)

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JP2003-358256 2003-10-17
PCT/JP2004/014156 WO2005037413A1 (ja) 2003-10-17 2004-09-28 多孔質膜カートリッジ

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EP (1) EP1683566A4 (de)
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Cited By (5)

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Publication number Priority date Publication date Assignee Title
US20120145704A1 (en) * 2009-09-02 2012-06-14 Nestec S.A. One-piece packaging comprising a container and a closure
CN105381642A (zh) * 2015-12-11 2016-03-09 浙江大学 一种用于低粘度液体的快速过滤装置
US10913060B2 (en) * 2011-11-10 2021-02-09 Biofire Diagnostics, Llc Loading vials
US10928116B2 (en) * 2019-02-27 2021-02-23 Electrolux Home Products, Inc. Modular water storage tank for a refrigerator
US12005401B2 (en) 2019-02-26 2024-06-11 Fujifilm Corporation Hydrophilic porous membrane and method for producing hydrophilic porous membrane

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Publication number Priority date Publication date Assignee Title
JP4551174B2 (ja) * 2004-09-30 2010-09-22 富士フイルム株式会社 多孔質膜カートリッジおよびその製造方法
JP2014030397A (ja) * 2012-08-03 2014-02-20 Toppan Printing Co Ltd 多孔質フィルターカラム、試薬カートリッジ、および核酸精製キット
EP3932528A4 (de) 2019-02-26 2022-04-20 FUJIFILM Corporation Hydrophile poröse membran und verfahren zur herstellung einer hydrophilen porösen membran

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US4014653A (en) * 1974-12-26 1977-03-29 Denver Chemical Manufacturing Company Micro-filter
US4287065A (en) * 1979-10-30 1981-09-01 Burron Medical Inc. Filter hub assembly
US5096575A (en) * 1988-02-12 1992-03-17 Schleicher & Schuell Gmbh Disposable filter unit
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120145704A1 (en) * 2009-09-02 2012-06-14 Nestec S.A. One-piece packaging comprising a container and a closure
US8820550B2 (en) * 2009-09-02 2014-09-02 Nestec S.A. One-piece packaging comprising a container and a closure
US10913060B2 (en) * 2011-11-10 2021-02-09 Biofire Diagnostics, Llc Loading vials
CN105381642A (zh) * 2015-12-11 2016-03-09 浙江大学 一种用于低粘度液体的快速过滤装置
US12005401B2 (en) 2019-02-26 2024-06-11 Fujifilm Corporation Hydrophilic porous membrane and method for producing hydrophilic porous membrane
US10928116B2 (en) * 2019-02-27 2021-02-23 Electrolux Home Products, Inc. Modular water storage tank for a refrigerator

Also Published As

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EP1683566A1 (de) 2006-07-26
WO2005037413A1 (ja) 2005-04-28
US20110072636A1 (en) 2011-03-31
EP1683566A4 (de) 2008-09-24
JPWO2005037413A1 (ja) 2007-11-22

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