US20030199521A1 - Compounds, methods and compositions useful for the treatment of bovine viral diarrhea virus (BVDV) infection and hepatitis C virus (HCV) infection - Google Patents

Compounds, methods and compositions useful for the treatment of bovine viral diarrhea virus (BVDV) infection and hepatitis C virus (HCV) infection Download PDF

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US20030199521A1
US20030199521A1 US10/044,315 US4431502A US2003199521A1 US 20030199521 A1 US20030199521 A1 US 20030199521A1 US 4431502 A US4431502 A US 4431502A US 2003199521 A1 US2003199521 A1 US 2003199521A1
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Christine Dykstra
Maurice Givens
David Stringfellow
Kenny Brock
David Boykin
Arvind Kumar
W. Wilson
Richard Tidwell
Chad Stephens
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Georgia State University Research Foundation Inc
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Assigned to GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC. reassignment GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: STEPHENS, CHAD E.
Publication of US20030199521A1 publication Critical patent/US20030199521A1/en
Priority to US10/796,657 priority patent/US7183286B2/en
Priority to US11/262,427 priority patent/US7163955B2/en
Priority to US11/601,889 priority patent/US7410999B2/en
Priority to US11/602,031 priority patent/US7410989B2/en
Assigned to NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT reassignment NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT EXECUTIVE ORDER 9424, CONFIRMATORY LICENSE Assignors: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
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    • A61K31/4164—1,3-Diazoles
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    • C07D207/04—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
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    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/22—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
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    • C07D307/10—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having no double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms
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    • C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
    • C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D307/38—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms
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    • C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
    • C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
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    • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
    • C07D333/06—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to the ring carbon atoms
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Definitions

  • This invention relates to the treatment of bovine viral diarrhea virus (BVDV) and hepatitis C virus (HCV) infections.
  • BVDV bovine viral diarrhea virus
  • HCV hepatitis C virus
  • Bovine viral diarrhea virus is an enveloped, single-stranded, positive sense RNA virus in the genus Pestivirus and the family Flaviviridae. Based on the presence or absence of visible cytopathic effect when susceptible cell monolayers are infected, two pathogenic biotypes of BVDV, referred to as cytopathic and noncytopathic, have been identified. Perdrizet J A in: B. P. Smith (ed), Large Animal Internal Medicine, First Edition (Mosby Press, St Louis, 731-737 (1990)). A differentiation is also made between biotypes of BVDV (referred to as biotypes I and II) based on certain viral RNA sequences in the 5′ untranslated region of the genome. Pellerin C, et al., Virology 203, 260-268 (1994); J. F. Ridpath et al., Virology 205, 66-74 (1994).
  • BVDV may cause acute infection in cattle, resulting in bovine respiratory disease, diarrhea and severe reproductive losses. Clinical symptoms of acute BVDV infection may range from the almost undetectable to the severe. Infection of pregnant cows and heifers may result in breeding problems (e.g., irregular heats), abortion, premature births or the birth of weak or stunted calves. In some cases, temporary damage to an animal's immune system may occur even when the clinical symptoms are not apparent. In addition to the illness caused by the virus itself, infected animals are more susceptible and are more likely to suffer from other diseases, such as pneumonia.
  • BVDV may also establish persistent infections. Potgieter, Vet. Clin. North Am. Food Anim. Pract 11, 501-520 (1995). Persistent BVDV infections are generally established via in utero infection of a developing fetus with a noncytopathic BVDV. The resulting animals are born immunotolerant of the particular BVDV by which they are infected, and may continually shed virus throughout their life span. While some persistently infected animals exhibit congenital malformations due to BVDV infection, many animals persistently infected with BVDV appear clinically normal. Baker, Rev. Sci. Tech 9, 25-41 (1990); Bielefeldt-Ohmann, Vet. Clin. North Am. Food Anim. Pract 11, 447-476 (1995). Persistently infected animals are thought to be the major disseminators of BVDV in the cattle population.
  • BVDV can be introduced into the embryo production system in association with gametes, serum, somatic cells, cumulus oocyte complexes (COCs), and result in contaminated in vitro fertilized (IVF) embryos or cell lines.
  • COCs cumulus oocyte complexes
  • IVF in vitro fertilized embryos or cell lines.
  • HCV hepatitis C virus
  • liver failure due to HCV is the presently the leading cause of liver transplants in the United States. It is suspected that there are, at present, more than 5 million people in the United States that are infected with HCV, and perhaps as many as 200 million around the world, making HCV infection a significant public health threat.
  • the nucleotide analog ribavirin (VIRAZOLE®/ICN Pharmaceuticals) has been shown to reduce concentrations of hepatitis C viral RNA in an infected subject, although at a slower rate than interferon alpha-2b. As with BVDV infection, a need exists for an effective treatment for HCV infection.
  • one aspect of the invention relates to novel compounds that are useful in treating members of the Flaviviridae family of viruses, such as bovine viral diarrhea virus (BVDV) infection and hepatitis C virus (HCV) infection.
  • BVDV bovine viral diarrhea virus
  • HCV hepatitis C virus
  • Compounds of the present invention will have a structure according to Formulas (I)-(VI), as follows:
  • X 1 and X 3 are each independently selected from the group consisting of O, S and NR 9 , wherein R 9 is H or alkyl;
  • X 2 and X 4 are each independently CH or N;
  • A is selected from the group consisting of H, alkyl, aryl,
  • R 1 , R 2 , R 3 , R 4 and R 5 are each independently selected from the group consisting of H, alkyl, alkoxy, amidine, halide, alkylhalide, nitro and amino groups;
  • R 6 is H, alkyl or aryl
  • R 7 and R 8 are each independently selected from the group consisting of H and alkyl.
  • compositions comprising a compound having a structure according to Formulas (I)-(VI), or a pharmaceutical salt thereof (i.e., an “active compound”), in a pharmaceutically acceptable carrier.
  • Pharmaceutical compositions of the present invention are useful in the treatment of bovine viral disease virus (BVDV) infection and hepatitis C virus (HCV) infection.
  • BVDV bovine viral disease virus
  • HCV hepatitis C virus
  • Certain aspects of the invention relate to methods of treating bovine viral disease virus (BVDV) infection in a subject in need of such treatment.
  • the method comprises administering to the subject a compound according to Formulas (I) through (VI), or a pharmaceutically acceptable salt thereof, in an amount effective to treat bovine viral disease virus (BVDV) infection.
  • HCV hepatitis C virus
  • the method comprises administering to the subject a compound according to Formulas (I) through (VI), or a pharmaceutically acceptable salt thereof, in an amount effective to treat hepatitis C virus (HCV) infection.
  • a further aspect of the present invention is the use of the active compounds described herein for the manufacture of a medicament for the treatment of bovine viral disease virus (BVDV) infection in a subject in need of such treatment.
  • BVDV bovine viral disease virus
  • Still another aspect of the present invention is the use of the active compounds described herein for the manufacture of a medicament for the treatment of treating hepatitis C virus (HCV) infection in a subject in need of such treatment.
  • HCV hepatitis C virus
  • FIG. 1 illustrates four chemical schemes useful in the synthesis of compounds of the present invention.
  • alkyl refers to C1-10 inclusive, linear, branched, or cyclic, saturated or unsaturated (i.e., alkenyl and alkynyl) hydrocarbon chains, including for example, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, hexyl, octyl, ethenyl, propenyl, butenyl, pentenyl, hexenyl, octenyl, butadienyl, propynyl, butynyl, pentynyl, hexynyl, heptynyl, and allenyl groups.
  • alkyl specifically includes cycloakyl hydrocarbon chains, which as used herein refers to C3 to C6 cyclic alkyl, such as cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. In the present invention, preferred alkyls are the lower alkyls.
  • lower alkyl refers to C1 to C4 linear or branched alkyl, such as methyl, ethyl, propyl, butyl, isopropyl, sec-butyl, and tert-butyl.
  • alkyl also encompasses substituted alkyls, which include aminoalkyls, hydroalkyls, oxygen-substituted alkyls (i.e., alkoxy groups), and halogen-substituted alkyls (i.e., alkyl halides, polyhaloalkyls).
  • aminoalkyl refers to C1 to C4 linear or branched amino-substituted alkyl, wherein the term “amino” refers to the group NR′R′′, and wherein R′ and R′′ are independently selected from H or lower alkyl as defined above, i.e., —NH 2 , —NHCH 3 , —N(CH 3 )2, etc.
  • hydroxyalkyl refers to C1 to C4 linear or branched hydroxy-substituted alkyl, i.e., —CH 2 OH, —(CH 2 ) 2 OH, etc.
  • alkoxy refers to C1 to C4 oxygen-substituted alkyl, i.e., —OCH 3 .
  • loweralkoxy refers to C1 to C4 linear or branched alkoxy, such as methoxy, ethoxy, propyloxy, butyloxy, isopropyloxy, and t-butyloxy.
  • halo and halide have their conventional meaning and refer to fluoro, chloro, bromo, and iodo groups. Preferred halo groups include chloro groups, and preferred alkyl halides of the present invention include CF 3 . “Nitro” groups, as used herein, have the structure —NO 2 .
  • aryl refers to C3 to C10 cyclic aromatic groups such as phenyl, naphthyl, and the like, and specifically includes substituted aryl groups including but not limited to tolyl, substituted phenyl, and substituted naphthyl.
  • Aryl groups may be substituted with halo, amino, nitro, and the like.
  • aryl Heterocyclic aromatic rings and polycyclic aromatic groups are also included in this definition of “aryl.” Specific examples of aryl groups encompassed by the present invention include but are not limited to cyclopentadienyl, phenyl, furan, thiophene, pyrrole, pyran, pyridine, imidazole, isothiazole, isoxazole, pyrazole, pyrazine, pyrimidine, and the like.
  • the compounds of the present invention are also useful in the form of their pharmaceutically acceptable salt forms.
  • Such salts may include, but are not limited to, the gluconate, lactate, acetate, tartarate, citrate, phosphate, borate, nitrate, sulfate, hydrobromide and hydrochloric salts of the compounds.
  • Compounds of Formulas (I)-(VI) and their pharmaceutically acceptable salts are referred to herein as “active compounds” or “active agents.”
  • the compounds represented by the Formulas (I) through (VI) may be formed by synthesis procedures that are described in the Examples below, as well as by certain methods known in the art. Some of these known methods are set forth below in the Examples by description or by reference (the disclosures of which are all incorporated herein by reference in their entirety).
  • bovine viral diarrhea virus infection means any infection (e.g., acute, latent or persistent) caused by a virus classified as a bovine viral disease virus (BVDV).
  • BVDV is an enveloped, single-stranded, positive sense RNA virus in the genus Pestivirus and the family Flaviviridae.
  • bovine viral disease virus (BVDV) encompasses all BVDV strains and all serotypes and variants thereof, including live, attenuated, killed or otherwise inactivated forms.
  • BVDV specifically includes cytopathic and noncytopathic strains, and strains of both biotype I and biotype II.
  • hepatitis C virus (HCV) infection includes any infections caused by the hepatitis C virus (HCV), which includes all strains, serotypes and variants of HCV.
  • a subject is administered a therapeutically-effective amount of the compound of formulas (I) through (VI), or a pharmaceutically acceptable salt thereof.
  • a “therapeutically-effective” amount as used herein is an amount of a compound of formulas (I) through (VI) that is sufficient to alleviate (e.g., mitigate, decrease, reduce) at least one of the symptoms associated with BVDV or HCV infection. It is not necessary that the administration of the compound eliminate the symptoms of BVDV or HCV, as long as the benefits of administration of compound outweigh the detriments.
  • BVDV and HCV are not intended to mean that the avian subject is necessarily cured of BVDV or HCV, or that all clinical signs thereof are eliminated, only that some alleviation or improvement in the condition of the subject is effected by administration of the compound of Formulas (I) through (VI).
  • Suitable subjects of the present invention include humans and animals.
  • mammals are preferred, with livestock (e.g., cattle, pigs, sheep, horses) and primates (e.g., monkeys, apes) being particularly preferred.
  • bovine subjects e.g., cows, bulls, calves
  • HCV infections humans are the preferred subjects.
  • Subjects may be adult, adolescent, juvenile, infant, or neonatal.
  • the subject is a live embryo, and may be in utero or in vitro (in the case of an embryo being maintained for in vitro fertilization).
  • Subjects may be administered the compounds and compositions of the present invention by any suitable means.
  • exemplary means are oral administration (e.g., in the form of a liquid or solid), intramuscular injection, subcutaneous injection, and intravenous injection.
  • Pharmaceutical formulations of the present invention comprise active compounds of the invention in a pharmaceutically acceptable carrier. Suitable pharmaceutical formulations include those suitable for inhalation, oral, rectal, topical, (including buccal, sublingual, dermal, vaginal and intraocular), parenteral (including subcutaneous, intradermal, intramuscular, intravenous and intraarticular) and transdermal administration.
  • the most suitable route of administration in any given case may depend upon the anatomic location of the condition being treated in the subject, the nature and severity of the condition being treated, and the particular active compound which is being used.
  • the formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art.
  • the compounds may be administered to the embryo by adding the active compound, in a suitable concentration, to the medium in which the embryo is being obtained.
  • active compounds or the pharmaceutically acceptable salts thereof are typically admixed with, inter alia, an acceptable carrier.
  • the carrier must, of course, be acceptable in the sense of being compatible with any other ingredients in the formulation and must not be deleterious to the patient.
  • the carrier may be a solid or a liquid, or both, and is preferably formulated with the compound as a unit-dose formulation, for example, a tablet, which may contain from 0.5% to 99% by weight of the active compound.
  • One or more active compounds may be incorporated in the formulations of the invention, which formulations may be prepared by any of the well known techniques of pharmacy consisting essentially of admixing the components, optionally including one or more accessory therapeutic ingredients.
  • Formulations suitable for oral administration may be presented in discrete units, such as capsules, cachets, lozenges, or tablets, each containing a predetermined amount of the active compound; as a powder or granules; as a solution or a suspension in an aqueous or non-aqueous liquid; or as an oil-in-water or water-in-oil emulsion.
  • Such formulations may be prepared by any suitable method of pharmacy which includes the step of bringing into association the active compound and a suitable carrier (which may contain one or more accessory ingredients as noted above).
  • the formulations of the invention are prepared by uniformly and intimately admixing the active compound with a liquid or finely divided solid carrier, or both, and then, if necessary, shaping the resulting mixture.
  • a tablet may be prepared by compressing or molding a powder or granules containing the active compound, optionally with one or more accessory ingredients.
  • Compressed tablets may be prepared by compressing, in a suitable machine, the compound in a free-flowing form, such as a powder or granules optionally mixed with a binder, lubricant, inert diluent, and/or surface active/dispersing agent(s).
  • Molded tablets may be made by molding, in a suitable machine, the powdered compound moistened with an inert liquid binder.
  • Formulations for oral administration may optionally include enteric coatings known in the art to prevent degradation of the formulation in the stomach and provide release of the drug in the small intestine.
  • Formulations of the present invention suitable for parenteral administration comprise sterile aqueous and non-aqueous injection solutions of the active compound, which preparations are preferably isotonic with the blood of the intended recipient. These preparations may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient.
  • Aqueous and non-aqueous sterile suspensions may include suspending agents and thickening agents.
  • the formulations may be presented in unit ⁇ dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example, saline or water-for-injection immediately prior to use.
  • sterile liquid carrier for example, saline or water-for-injection immediately prior to use.
  • Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described.
  • an injectable, stable, sterile composition comprising a compound of Formula (I)-Formula (VI), or a salt thereof, in a unit dosage form in a sealed container.
  • the compound or salt is provided in the form of a lyophilizate which is capable of being reconstituted with a suitable pharmaceutically acceptable carrier to form a liquid composition suitable for injection thereof into a subject.
  • the unit dosage form typically comprises from about 10 mg to about 10 grams of the compound or salt.
  • a sufficient amount of emulsifying agent which is physiologically acceptable may be employed in sufficient quantity to emulsify the compound or salt in an aqueous carrier.
  • the present invention provides liposomal formulations of the compounds disclosed herein and salts thereof.
  • the technology for forming liposomal suspensions is well known in the art.
  • the compound or salt thereof is an aqueous-soluble salt, using conventional liposome technology, the same may be incorporated into lipid vesicles. In such an instance, due to the water solubility of the compound or salt, the compound or salt will be substantially entrained within the hydrophilic center or core of the liposomes.
  • the lipid layer employed may be of any conventional composition and may either contain cholesterol or may be cholesterol-free.
  • the salt may be substantially entrained within the hydrophobic lipid bilayer which forms the structure of the liposome.
  • the liposomes which are produced may be reduced in size, as through the use of standard sonication and homogenization techniques.
  • the liposomal formulations containing the pharmaceutically active compounds identified with the methods described herein may be lyophilized to produce a lyophilizate which may be reconstituted with a pharmaceutically acceptable carrier, such as water, to regenerate a liposomal suspension.
  • the pharmaceutical formulations may contain other additives, such as pH-adjusting additives.
  • useful pH-adjusting agents include acids, such as hydrochloric acid, bases or buffers, such as sodium lactate, sodium acetate, sodium phosphate, sodium citrate, sodium borate, or sodium gluconate.
  • the compositions may contain microbial preservatives.
  • Useful microbial preservatives include methylparaben, propylparaben, and benzyl alcohol. The microbial preservative is typically employed when the formulation is placed in a vial designed for multidose use.
  • the pharmaceutical formulations of the present invention may be lyophilized using techniques well known in the art.
  • compositions of the present invention may comprise compounds of the present invention in lyophilized form.
  • pharmaceutical formulations of the present invention may comprise compounds of the present invention in a pharmaceutically acceptable carrier.
  • Such pharmaceutical formulations are generally made by admixing the compounds described herein with a pharmaceutically acceptable carrier.
  • Pharmaceutically acceptable carriers are preferably liquid, particularly aqueous, carriers, the selection of which are known in the art.
  • the compound may be mixed in a buffered saline (e.g., pH 6 to 8) or conventional culture media.
  • the formulation may be stored in a sterile glass container sealed with a rubber stopper through which liquids may be injected and formulation withdrawn by syringe.
  • a therapeutically effective dosage of any specific compound may vary somewhat from compound to compound and subject to subject, and will depend upon the condition of the subject and the route of delivery.
  • a dosage from about 1 mg/kg to about 15 mg/kg of subject body weight, or about 20 mg/kg of subject body weight, or even about 25 mg/kg of subject body weight may be employed for intravenous injection or oral administration.
  • concentration of the compound of the present invention or a pharmaceutically acceptable salt thereof in a formulation of the present invention may be determined by the skilled artisan and will vary according to certain conditions, including the characteristics of subject being treated (e.g., species, age, weight), the severity and type of the infecting virus or the strain that the subject is being vaccinated against, the dosage form being used, and the like.
  • the compounds of the present invention may be administered in conjunction with other antiviral compounds, as may be determined by the skilled artisan.
  • compound numbers refer to compounds with structures that are set forth in FIG. 1.
  • the mono salt (0.65 g) was dissolved in 120 ml of ethanol and acidified with HCl-saturated ethanol and after standing overnight in a refrigerator the resultant solid was filtered, washed with ether and dried for 24 h in a vacuum oven at 70° C. to yield 0.6 g (85%) mp 300° C.
  • the mono salt was dissolved in 100 ml of ethanol and acidified with HCl-saturated ethanol and after cooling in an ice bath the resultant solid was filtered, washed with ether and dried for 24 h in a vacuum oven at 75° C. to yield 0.7 g (91%) mp>300° C.
  • MEM-eq minimum essential medium (MEM) with Earle's salts supplemented with 10% (v/v) equine serum, sodium bicarbonate (0.75 mg/ml), L-glutamine (0.29 mg/ml), penicillin G (100 U/ml), streptomycin (100 ⁇ g/ml), and amphotericin B (0.25 ⁇ g/ml)), which was derived by trypsinization of a confluent monolayer of Madin Darby Bovine Kidney (MDBK) cells in a 25 cm 2 flask. Cells were incubated at 38.5° C. with 5% CO 2 for 24 hours. The average number of cells per well was determined and later used to calculate appropriate multiplicities of infection (MOI) of BVDV virus.
  • MEM-eq minimum essential medium
  • MDBK Madin Darby Bovine Kidney
  • the MDBK cells were resuspended in MEM-eq with no antiviral compound.
  • Uterine tubal cells (UTC) were freeze-thawed and stored at ⁇ 80° C. for analysis.
  • UTC lysates were serially diluted with medium from Day 7 and assayed by immunoperoxidase for the presence of BVDV.
  • peroxidase-conjugated rabbit anti-mouse IgG (Jackson Immuno Research Lab, West Grove, Pa.) was added. After a short incubation period, unbound peroxidase-conjugated antibody was removed by washing with PBS and Tween 20. Finally, the enzyme substrate, aminoethyl carbazole (Zymed Laboratories, Inc., South San Francisco, Calif.), which produces a reddish-brown color when oxidized by horseradish peroxidase, was added. Color change was visualized under light microscopy and compared to known positive and negative controls on each plate.
  • RNA samples were stored at ⁇ 80° C. until RT-nPCR was performed.
  • the initial reverse transcription polymerase chain reaction was performed in the bottom of the tubes containing the dried trehalose mixture within the lid.
  • Two ⁇ L of RNA were added through the overlaid mineral oil (50 ⁇ L) to the initial reaction volume (48 ⁇ L) containing the following reagents (Promega): 5 ⁇ L 10 ⁇ buffer, 8 ⁇ L of MgCl2 (25 mM), 2 ⁇ L of dNTPs (10 mM), 1 ⁇ L of each outer primer BVD 100 and HCV 368 (5 ⁇ M), 1 ⁇ L of Triton X-100 (10% stock), 0.25 ⁇ L of dithiothreitol (100 mM), 0.25 ⁇ L (10 U) RNAsin, 0.5 ⁇ L (2.5 U) of Taq polymerase, and 0.5 ⁇ L (100 U) of MMLV (Moloney Murine Leukemia Virus) reverse transcriptase.
  • the tubes were then subjected to the following reagent
  • a final elongation step of 72° C. for 10 min completed the initial amplification reaction.
  • the tubes were inverted several times to mix the samples in the lid and in the base to initiate the nested polymerase chain reaction (nPCR).
  • the tubes were then centrifuged at 14,000 ⁇ g for 12 sec before returning to the thermocycler for nPCR, using 30 cycles of 94° C. for 1 min, 55° C. for 1 min and 72° C. for 45 sec.
  • a final elongation step of 72° C. for 10 min completed the amplification process prior to maintaining the reactions at 4° C.
  • Five microliter aliquots of PCR products were separated by 1.5% agarose gel electrophoresis.
  • the agarose gels contained 0.5 ⁇ g/ml ethidium bromide to allow visualization of RT-nPCR products using an ultraviolet transilluminator.
  • the outer primers, BVD 100 (5′-GGCTAGCCATGCCCTTAG-3′) (SEQ ID NO. 1) and HCV 368 (5′-CCATGTGCCATGTACAG-3′) (SEQ ID NO. 2) amplified a 290 base pair sequence of the 5′ untranslated region of the viral genome.
  • the inner primers, BVD 180 (5′-CCTGAGTACAGGGDAGT CGTCA-3′) (SEQ ID NO. 3) and HCV 368 amplified a 213 base pair sequence within the first amplicon.
  • Cow ovaries were collected at an abattoir in Omaha, Neb., and placed in PBS for transport to a nearby laboratory.
  • the contents of 1- to 10-mm follicles were aspirated at a vacuum rate of 21.5 ml/min and poured onto a 70 ⁇ m filter.
  • Cellular components of the pooled follicular aspirate were rinsed with TL-HEPES and searched for oocytes surrounded by multiple layers of dense cumulus cells.
  • Useable cumulus oocyte complexes (COCs) were washed two additional times in TL-HEPES, then placed in 7.5 ml of maturation media that had previously equilibrated at 38.5° C. in an atmosphere of 5% CO 2 and humidified air. The maturation media was then sealed and maintained at 38.5° C. for 20 to 22 h while being transported to the experimental laboratory.
  • Oocytes were matured in cell culture medium 199 (CCM 199) with Earle's salts (GIBCO-BRL, Grand Island, N.Y., USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; HyClone Lab., Inc., Logan, Utah, USA), sodium pyruvate (11 ⁇ g/ml), bovine FSH (0.01 U/ml), bovine LH (0.01 U/ml), penicillin (100 U/ml) and streptomycin (100 ⁇ g/ml).
  • Matured oocytes were fertilized in CR2 medium (C. F. Rosenkrans et al., Theriogenology 35, 266 (1991)) supplemented with BSA (6 mg/ml), heparin (10 ⁇ g/ml), penicillamine (0.3 ⁇ g/ml), hypotaurine (0.2 ⁇ g/ml), penicillin (100 ⁇ /ml) and streptomycin (100 ⁇ g/ml).
  • IVC in vitro culture
  • BVDV Bovine Viral Diarrhea Virus
  • the noncytopathic strains of BVDV used in this research included 2 diverse Genotype I strains (SD-1 and NY-1) and 2 diverse Genotype II strains (CD-87 and PA-131). Givens M D et al., Theriogenology 2000;54:1093-1107. All stocks were propagated in BVDV-free MDBK cells cultured in MEM-eq. Virus was harvested by freezing and thawing and was stored in cryovials at ⁇ 80° C. until needed.
  • the nearly nude embryos were examined for cleavage, and those at the 5-cell stage or greater were washed 1 more time in IVC medium and placed with pieces of detached cumulus in 60- ⁇ l drops (20 to 25 per drop) of the IVC medium with 10% (v/v) FBS under mineral oil. These developed embryos were incubated an additional 4 d. After the final 4 d in IVC, embryos were transferred into 3 ml of MEM-eq, separated from cumulus cells, and development to the morula or blastocyst stage was noted.
  • tissue culture infective dose 50% (TCID 50 )/ml of the exposure aliquot was determined by the method of Reed and Muench (L. J. Reed and H. Muench, Am J Hygiene 27, 493-497 (1938). Results of viral detection assays were compared using a Pearson Chi-square test statistic (J. Sall and A. Lehman, JMP Start Statistics (Duxbury Press, Belmont, Calif. (1996),195-211).
  • Table 2 sets forth the results of the analysis of in vitro culture media and cell lysates that have been treated with the indicated antiviral compound for the indicated time at a concentration of 12.5 ⁇ M, after exposure to BVDV at a MOI of 0.05 (see Example 12).
  • Table 3 sets forth the results of the analysis of in vitro culture media and cell lysates that have been treated with the indicated antiviral compound for the indicated time at a concentration of 12.5 ⁇ M, after exposure to BVDV at a MOI of 1.0 (see Example 12).
  • Table 4 sets forth the results of the analysis of Day 3 in vitro culture media and Day 3 cell lysate that has been treated with the indicated antiviral compound at a concentration of 12.5 ⁇ M, after exposure to BVDV at a MOI of 0.5 (see Example 12).
  • Table 5 sets forth the results of the analysis of Day 3 cell lysates that have been treated with the indicated antiviral compound for three days at the indicated concentration, after exposure to BVDV at a MOI of 0.05.
  • TABLE 5 Day 3 Media Day 3 Cell Lysates Antiviral drug TCID 50 /mL % Control TCID 50 /mL % Control No antiviral 2.00E + 06 3.50E + 05 DB 456 25 ⁇ m 3.50E + 03 0.1750% 2.00E + 03 0.5714% DB 456 12 ⁇ m 6.20E + 02 0.0310% 3.50E + 03 1.0000% DB 456 6 ⁇ m 3.50E + 04 1.7500% 3.50E + 04 10.0000% DB 456 3 ⁇ m 3.50E + 05 17.5000% 6.20E + 05 177.1429% DB 456 1.5 ⁇ m 5.10E + 05 25.5000% 3.50E + 06 1000.0000% DB 456 0.7 ⁇ m 6.30E + 05 31.500

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