UA64696C2 - Enzyme degrading rhamnogalacturonane ii and derivatives thereof, methods for preparing this enzyme and rhamnogalacturonane ii - Google Patents
Enzyme degrading rhamnogalacturonane ii and derivatives thereof, methods for preparing this enzyme and rhamnogalacturonane ii Download PDFInfo
- Publication number
- UA64696C2 UA64696C2 UA97126189A UA97126189A UA64696C2 UA 64696 C2 UA64696 C2 UA 64696C2 UA 97126189 A UA97126189 A UA 97126189A UA 97126189 A UA97126189 A UA 97126189A UA 64696 C2 UA64696 C2 UA 64696C2
- Authority
- UA
- Ukraine
- Prior art keywords
- enzyme
- microorganisms
- differs
- degradation
- chromatography
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 78
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 78
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000000593 degrading effect Effects 0.000 title claims abstract description 21
- 230000000694 effects Effects 0.000 claims abstract description 42
- 244000005700 microbiome Species 0.000 claims abstract description 34
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 18
- 108090000604 Hydrolases Proteins 0.000 claims abstract description 14
- 102000004157 Hydrolases Human genes 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims description 47
- 239000000284 extract Substances 0.000 claims description 35
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 27
- 238000004519 manufacturing process Methods 0.000 claims description 23
- 241000233866 Fungi Species 0.000 claims description 22
- 239000001963 growth medium Substances 0.000 claims description 20
- 239000002609 medium Substances 0.000 claims description 15
- 239000011347 resin Substances 0.000 claims description 13
- 229920005989 resin Polymers 0.000 claims description 13
- 238000000227 grinding Methods 0.000 claims description 12
- 238000005377 adsorption chromatography Methods 0.000 claims description 9
- 238000000108 ultra-filtration Methods 0.000 claims description 8
- 239000000178 monomer Substances 0.000 claims description 7
- 238000001556 precipitation Methods 0.000 claims description 7
- 239000000539 dimer Substances 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 5
- 238000005571 anion exchange chromatography Methods 0.000 claims description 5
- 238000002270 exclusion chromatography Methods 0.000 claims description 4
- 229920002521 macromolecule Polymers 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 3
- 238000005349 anion exchange Methods 0.000 claims description 2
- 239000004793 Polystyrene Substances 0.000 claims 1
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 claims 1
- 239000000419 plant extract Substances 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 2
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 description 75
- 238000006731 degradation reaction Methods 0.000 description 75
- 229940088598 enzyme Drugs 0.000 description 62
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 53
- 230000002255 enzymatic effect Effects 0.000 description 52
- 229920001282 polysaccharide Polymers 0.000 description 39
- 239000005017 polysaccharide Substances 0.000 description 39
- 150000004676 glycans Chemical class 0.000 description 38
- 235000014101 wine Nutrition 0.000 description 37
- 241000196324 Embryophyta Species 0.000 description 33
- 239000000243 solution Substances 0.000 description 20
- 239000000203 mixture Substances 0.000 description 18
- 239000002253 acid Substances 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 17
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 15
- 238000001228 spectrum Methods 0.000 description 14
- 235000012055 fruits and vegetables Nutrition 0.000 description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- 239000007853 buffer solution Substances 0.000 description 12
- 210000000170 cell membrane Anatomy 0.000 description 11
- 235000013399 edible fruits Nutrition 0.000 description 11
- 230000012010 growth Effects 0.000 description 11
- 235000013311 vegetables Nutrition 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 238000011534 incubation Methods 0.000 description 10
- 239000001814 pectin Substances 0.000 description 10
- 229920001277 pectin Polymers 0.000 description 10
- 235000010987 pectin Nutrition 0.000 description 10
- 101100167439 Arabidopsis thaliana CLPC1 gene Proteins 0.000 description 9
- 101100509022 Arabidopsis thaliana IRM1 gene Proteins 0.000 description 9
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 9
- 244000141359 Malus pumila Species 0.000 description 9
- 230000009471 action Effects 0.000 description 9
- 239000012141 concentrate Substances 0.000 description 9
- 238000010828 elution Methods 0.000 description 9
- 238000000746 purification Methods 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 238000005119 centrifugation Methods 0.000 description 8
- 239000003245 coal Substances 0.000 description 8
- 230000007515 enzymatic degradation Effects 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 235000020095 red wine Nutrition 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 7
- 150000007513 acids Chemical class 0.000 description 7
- 239000000084 colloidal system Substances 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 239000003814 drug Substances 0.000 description 7
- 239000012528 membrane Substances 0.000 description 7
- 150000002772 monosaccharides Chemical class 0.000 description 7
- 230000007935 neutral effect Effects 0.000 description 7
- 230000002351 pectolytic effect Effects 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 6
- 235000009754 Vitis X bourquina Nutrition 0.000 description 6
- 235000012333 Vitis X labruscana Nutrition 0.000 description 6
- 240000006365 Vitis vinifera Species 0.000 description 6
- 235000014787 Vitis vinifera Nutrition 0.000 description 6
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 6
- 235000015197 apple juice Nutrition 0.000 description 6
- 235000021016 apples Nutrition 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 230000000717 retained effect Effects 0.000 description 6
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical group OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 description 5
- 244000000626 Daucus carota Species 0.000 description 5
- 235000002767 Daucus carota Nutrition 0.000 description 5
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 5
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 5
- 230000007717 exclusion Effects 0.000 description 5
- 230000002538 fungal effect Effects 0.000 description 5
- 229930182830 galactose Natural products 0.000 description 5
- 229960003082 galactose Drugs 0.000 description 5
- 241000894007 species Species 0.000 description 5
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 5
- 235000000346 sugar Nutrition 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 235000016068 Berberis vulgaris Nutrition 0.000 description 4
- 241000335053 Beta vulgaris Species 0.000 description 4
- 101001096557 Dickeya dadantii (strain 3937) Rhamnogalacturonate lyase Proteins 0.000 description 4
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 4
- 108010059820 Polygalacturonase Proteins 0.000 description 4
- 241000219094 Vitaceae Species 0.000 description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 108010093305 exopolygalacturonase Proteins 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 235000021021 grapes Nutrition 0.000 description 4
- 229920003053 polystyrene-divinylbenzene Polymers 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 description 3
- 235000011430 Malus pumila Nutrition 0.000 description 3
- 235000015103 Malus silvestris Nutrition 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 239000004098 Tetracycline Substances 0.000 description 3
- 239000008351 acetate buffer Substances 0.000 description 3
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 230000003413 degradative effect Effects 0.000 description 3
- 229940079919 digestives enzyme preparation Drugs 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 229940097043 glucuronic acid Drugs 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 238000002803 maceration Methods 0.000 description 3
- 230000011987 methylation Effects 0.000 description 3
- 238000007069 methylation reaction Methods 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 239000009754 rhamnogalacturonan I Substances 0.000 description 3
- 239000008914 rhamnogalacturonan II Substances 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 239000001632 sodium acetate Substances 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- 125000001424 substituent group Chemical group 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- 229960002180 tetracycline Drugs 0.000 description 3
- 229930101283 tetracycline Natural products 0.000 description 3
- 235000019364 tetracycline Nutrition 0.000 description 3
- 244000019459 Cynara cardunculus Species 0.000 description 2
- 235000019106 Cynara scolymus Nutrition 0.000 description 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- HMFHBZSHGGEWLO-HWQSCIPKSA-N L-arabinofuranose Chemical compound OC[C@@H]1OC(O)[C@H](O)[C@H]1O HMFHBZSHGGEWLO-HWQSCIPKSA-N 0.000 description 2
- 241000446313 Lamella Species 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 241000218922 Magnoliophyta Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000002730 additional effect Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 235000016520 artichoke thistle Nutrition 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 235000013405 beer Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005352 clarification Methods 0.000 description 2
- 239000002361 compost Substances 0.000 description 2
- 238000010908 decantation Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 235000021186 dishes Nutrition 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 235000020400 fruit nectar Nutrition 0.000 description 2
- 229940059442 hemicellulase Drugs 0.000 description 2
- 108010002430 hemicellulase Proteins 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 235000015110 jellies Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 238000012916 structural analysis Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 235000020097 white wine Nutrition 0.000 description 2
- SLYBRYXJOHULJH-BDVNFPICSA-N (2S,3R,4R,5S)-2,3,4,5-tetrahydroxy-2-methylhexanal Chemical compound C[C@H](O)[C@@H](O)[C@@H](O)[C@](C)(O)C=O SLYBRYXJOHULJH-BDVNFPICSA-N 0.000 description 1
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
- ALNDFFUAQIVVPG-SRQIZXRXSA-N 2-O-Methyl-D-xylose Chemical compound CO[C@@H](C=O)[C@@H](O)[C@H](O)CO ALNDFFUAQIVVPG-SRQIZXRXSA-N 0.000 description 1
- GSCHIGXDTVYEEM-UHFFFAOYSA-N 2-[2-[[3-[6-[[4,5-dihydroxy-3-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxymethyl]-3,4-dihydroxy-5-[3,4,5-trihydroxy-6-[(3,4,5-trihydroxyoxan-2-yl)oxymethyl]oxan-2-yl]oxyoxan-2-yl]oxy-4,5-dihydroxy-6-[4,5,6-trihydroxy-2-(hydroxymethyl)oxan Chemical group OC1C(O)C(O)C(CO)OC1OC1C(OCC2C(C(O)C(O)C(OC3C(OC(O)C(O)C3O)CO)O2)OC2C(C(O)C(OC3C(C(O)C(O)C(COC4C(C(O)C(O)CO4)O)O3)O)C(COC3C(C(O)C(O)CO3)OC3C(C(O)C(O)C(CO)O3)O)O2)O)OCC(O)C1O GSCHIGXDTVYEEM-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-M 3-carboxy-2,3-dihydroxypropanoate Chemical class OC(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-M 0.000 description 1
- PAIOZXJXGSOKFN-UHFFFAOYSA-N Aceric acid Natural products CC1OC(O)C(O)C1(O)C(O)=O PAIOZXJXGSOKFN-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102100037435 Antiviral innate immune response receptor RIG-I Human genes 0.000 description 1
- 101710127675 Antiviral innate immune response receptor RIG-I Proteins 0.000 description 1
- 229920000189 Arabinogalactan Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000021537 Beetroot Nutrition 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- AVGPOAXYRRIZMM-UHFFFAOYSA-N D-Apiose Natural products OCC(O)(CO)C(O)C=O AVGPOAXYRRIZMM-UHFFFAOYSA-N 0.000 description 1
- ASNHGEVAWNWCRQ-LJJLCWGRSA-N D-apiofuranose Chemical compound OC[C@@]1(O)COC(O)[C@@H]1O ASNHGEVAWNWCRQ-LJJLCWGRSA-N 0.000 description 1
- ASNHGEVAWNWCRQ-UHFFFAOYSA-N D-apiofuranose Natural products OCC1(O)COC(O)C1O ASNHGEVAWNWCRQ-UHFFFAOYSA-N 0.000 description 1
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102100024023 Histone PARylation factor 1 Human genes 0.000 description 1
- 101001005668 Homo sapiens Mastermind-like protein 3 Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 102100025134 Mastermind-like protein 3 Human genes 0.000 description 1
- ALNDFFUAQIVVPG-UHFFFAOYSA-N O2-methyl-D-xylose Natural products COC(C=O)C(O)C(O)CO ALNDFFUAQIVVPG-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 241001311547 Patina Species 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- GEEIPHPXFCSZGY-WTFGDDLMSA-N [(2S,3S,4S,5S)-5-acetyloxy-2,3,4-trimethoxyhexyl] acetate Chemical compound CC(=O)OC[C@H](OC)[C@H](OC)[C@@H](OC)[C@H](C)OC(C)=O GEEIPHPXFCSZGY-WTFGDDLMSA-N 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- VZTDIZULWFCMLS-UHFFFAOYSA-N ammonium formate Chemical compound [NH4+].[O-]C=O VZTDIZULWFCMLS-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000019312 arabinogalactan Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000011210 chromatographic step Methods 0.000 description 1
- 239000012501 chromatography medium Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 201000003146 cystitis Diseases 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000008380 degradant Substances 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 235000013367 dietary fats Nutrition 0.000 description 1
- 230000005218 dilaceration Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000002243 furanoses Chemical class 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000010520 ghee Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- LUEWUZLMQUOBSB-GFVSVBBRSA-N mannan Chemical class O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-GFVSVBBRSA-N 0.000 description 1
- 108010005335 mannoproteins Proteins 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000007003 mineral medium Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 235000008486 nectar Nutrition 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- -1 peats Substances 0.000 description 1
- 108020004410 pectinesterase Proteins 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 108010070456 protopectinase Proteins 0.000 description 1
- 150000003214 pyranose derivatives Chemical class 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000010801 sewage sludge Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- CMJCEVKJYRZMIA-UHFFFAOYSA-M thallium(i) iodide Chemical compound [Tl]I CMJCEVKJYRZMIA-UHFFFAOYSA-M 0.000 description 1
- 125000000026 trimethylsilyl group Chemical group [H]C([H])([H])[Si]([*])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000005292 vacuum distillation Methods 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D41/00—Regeneration of the filtering material or filter elements outside the filter for liquid or gaseous fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B7/00—Cleaning by methods not provided for in a single other subclass or a single group in this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G1/00—Preparation of wine or sparkling wine
- C12G1/02—Preparation of must from grapes; Must treatment and fermentation
- C12G1/0203—Preparation of must from grapes; Must treatment and fermentation by microbiological or enzymatic treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
- C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
- C12H1/003—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages by a biochemical process
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01171—Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2321/00—Details relating to membrane cleaning, regeneration, sterilization or to the prevention of fouling
- B01D2321/16—Use of chemical agents
- B01D2321/166—Use of enzymatic agents
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Physiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9506142A FR2734578B1 (fr) | 1995-05-23 | 1995-05-23 | Enzyme degradant le rhamnogalacturonane ii, procedes d'obtention de cette enzyme et du rhamnogalacturonane ii et utilisation de cette enzyme |
PCT/FR1996/000758 WO1996037604A2 (fr) | 1995-05-23 | 1996-05-21 | Enzyme et microorganisme degradant le rhamnogalacturonane ii |
Publications (1)
Publication Number | Publication Date |
---|---|
UA64696C2 true UA64696C2 (en) | 2004-03-15 |
Family
ID=9479302
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UA97126189A UA64696C2 (en) | 1995-05-23 | 1996-05-21 | Enzyme degrading rhamnogalacturonane ii and derivatives thereof, methods for preparing this enzyme and rhamnogalacturonane ii |
Country Status (20)
Country | Link |
---|---|
US (1) | US6103506A (xx) |
EP (1) | EP0827533B1 (xx) |
JP (1) | JPH11508127A (xx) |
AR (1) | AR002067A1 (xx) |
AT (1) | ATE330006T1 (xx) |
AU (1) | AU6006796A (xx) |
BR (1) | BR9608812A (xx) |
CA (1) | CA2222193A1 (xx) |
CZ (1) | CZ370197A3 (xx) |
DE (1) | DE69636247D1 (xx) |
FR (1) | FR2734578B1 (xx) |
HU (1) | HUP9900744A2 (xx) |
IL (1) | IL118404A (xx) |
PL (1) | PL323529A1 (xx) |
RU (1) | RU2220203C2 (xx) |
SK (1) | SK156697A3 (xx) |
TR (1) | TR199701421T1 (xx) |
UA (1) | UA64696C2 (xx) |
WO (1) | WO1996037604A2 (xx) |
ZA (1) | ZA964107B (xx) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7132119B1 (en) | 1997-04-08 | 2006-11-07 | Pall Corporation | Method for producing beer |
WO1999038956A2 (en) * | 1998-01-30 | 1999-08-05 | Institut National De La Recherche Agronomique | Enzyme modifying rhamnogalacturonane ii, dna encoding for said enzymes and method for producing the enzyme |
FR2980800B1 (fr) * | 2011-09-30 | 2014-01-10 | Agronomique Inst Nat Rech | Procede de preparation d'un produit alimentaire liquide enrichi en oligosaccharides et en polysaccharides |
JP6539400B1 (ja) * | 2018-10-24 | 2019-07-03 | 株式会社アンチエイジング・プロ | Lps高含有組成物の製造方法 |
CN117204574B (zh) * | 2023-11-09 | 2024-02-02 | 北京市农林科学院 | 促进肠道菌群发酵平衡的果胶基膳食补充剂及其制备方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3541945A1 (de) * | 1985-11-27 | 1987-06-04 | Lomapharm Rudolf Lohman Gmbh K | Immunstimulierend wirkende polysaccharide aus zellkulturen von echinacea purpurea (l.) moench und echinacea angustifolia, d.c. verfahren zu ihrer herstellung und sie enthaltende pharmazeutische zubereitungen |
DE3934304A1 (de) * | 1989-10-13 | 1991-04-18 | Lohmann Rudolf Lomapharm | Polysaccharide mit antiphlogistischer wirkung, verfahren zu ihrer gewinnung und sie enthaltende arzneimittel |
JPH06506831A (ja) * | 1991-05-02 | 1994-08-04 | ノボザイムス アクティーゼルスカブ | ラムノガラクツロナーゼ、対応するdna配列、ラムノガラクツロナーゼ含有酵素調製物および該酵素調製物の用途 |
EP0570075A3 (en) * | 1992-05-15 | 1995-02-15 | Quest Int | Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalacturonase activity. |
DE4221753C2 (de) * | 1992-07-02 | 1994-11-24 | Plantamed Arzneimittel Gmbh | Immunologisch und antiphlogistisch wirksame Polysaccharide aus Sedum telephium, Verfahren zu ihrer Gewinnung und diese enthaltende Arzneimittel |
DK24493D0 (xx) * | 1993-03-05 | 1993-03-05 | Novo Nordisk As |
-
1995
- 1995-05-23 FR FR9506142A patent/FR2734578B1/fr not_active Expired - Fee Related
-
1996
- 1996-05-21 PL PL96323529A patent/PL323529A1/xx unknown
- 1996-05-21 HU HU9900744A patent/HUP9900744A2/hu unknown
- 1996-05-21 JP JP8535433A patent/JPH11508127A/ja active Pending
- 1996-05-21 SK SK1566-97A patent/SK156697A3/sk unknown
- 1996-05-21 EP EP96917526A patent/EP0827533B1/fr not_active Expired - Lifetime
- 1996-05-21 AU AU60067/96A patent/AU6006796A/en not_active Abandoned
- 1996-05-21 WO PCT/FR1996/000758 patent/WO1996037604A2/fr active IP Right Grant
- 1996-05-21 CA CA002222193A patent/CA2222193A1/fr not_active Abandoned
- 1996-05-21 DE DE69636247T patent/DE69636247D1/de not_active Expired - Lifetime
- 1996-05-21 BR BR9608812A patent/BR9608812A/pt not_active Application Discontinuation
- 1996-05-21 RU RU97121499/13A patent/RU2220203C2/ru not_active IP Right Cessation
- 1996-05-21 CZ CZ973701A patent/CZ370197A3/cs unknown
- 1996-05-21 US US08/973,335 patent/US6103506A/en not_active Expired - Fee Related
- 1996-05-21 TR TR97/01421T patent/TR199701421T1/xx unknown
- 1996-05-21 UA UA97126189A patent/UA64696C2/uk unknown
- 1996-05-21 AT AT96917526T patent/ATE330006T1/de not_active IP Right Cessation
- 1996-05-22 AR ARP960102653A patent/AR002067A1/es active IP Right Grant
- 1996-05-22 ZA ZA964107A patent/ZA964107B/xx unknown
- 1996-05-23 IL IL118404A patent/IL118404A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AR002067A1 (es) | 1998-01-07 |
CA2222193A1 (fr) | 1996-11-28 |
CZ370197A3 (cs) | 1998-03-18 |
US6103506A (en) | 2000-08-15 |
WO1996037604A2 (fr) | 1996-11-28 |
IL118404A0 (en) | 1996-09-12 |
PL323529A1 (en) | 1998-03-30 |
ATE330006T1 (de) | 2006-07-15 |
WO1996037604A3 (fr) | 1997-01-23 |
SK156697A3 (en) | 1998-09-09 |
TR199701421T1 (xx) | 1998-02-21 |
AU6006796A (en) | 1996-12-11 |
EP0827533B1 (fr) | 2006-06-14 |
DE69636247D1 (de) | 2006-07-27 |
EP0827533A2 (fr) | 1998-03-11 |
ZA964107B (en) | 1996-12-03 |
HUP9900744A2 (hu) | 1999-06-28 |
FR2734578B1 (fr) | 1997-08-14 |
FR2734578A1 (fr) | 1996-11-29 |
RU2220203C2 (ru) | 2003-12-27 |
BR9608812A (pt) | 1999-02-17 |
JPH11508127A (ja) | 1999-07-21 |
IL118404A (en) | 2006-10-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Yapo | Pectic substances: From simple pectic polysaccharides to complex pectins—A new hypothetical model | |
Øbro et al. | Rhamnogalacturonan I in Solanum tuberosum tubers contains complex arabinogalactan structures | |
Sieiro et al. | Microbial pectic enzymes in the food and wine industry | |
Patil et al. | Debittering of citrus fruit juice by naringinase of Penicillium purpurogenum | |
US5591620A (en) | Methods of detecting and isolating a ripening form of a polypeptide having rhamnogalacturonase activity | |
Ire et al. | Production, purification and characterization of polygalacturonase from Aspergillus niger in solid state and submerged fermentation using banana peels | |
UA64696C2 (en) | Enzyme degrading rhamnogalacturonane ii and derivatives thereof, methods for preparing this enzyme and rhamnogalacturonane ii | |
JPH06506831A (ja) | ラムノガラクツロナーゼ、対応するdna配列、ラムノガラクツロナーゼ含有酵素調製物および該酵素調製物の用途 | |
WO2012038509A2 (en) | Methods of juice production | |
Ezugwu et al. | Properties and application of pectinase obtained from Galactomyces candidum using pectin extracted from mango peels as a carbon source | |
Hadj-Taieb et al. | Fermentor production of pectinases on gruel, a local by-product and their use in olive oil extraction | |
KR101609606B1 (ko) | 말똥진흙버섯의 총 트리테르펜 화합물 생산량을 향상시키는 방법 | |
JP3899462B2 (ja) | ガン細胞アポトーシス誘導剤 | |
EP2376621B1 (fr) | Souche bacterienne et melange bacterien a activite fucanolytique | |
CN1189185A (zh) | 降解鼠李半乳糖醛酸聚糖ⅱ的酶和微生物 | |
KR20210025528A (ko) | 발효 셀룰로오스 함유 식초 및 그 제조 방법 | |
CN107151631A (zh) | 一种高产果胶酶菌株及其应用 | |
KR100820291B1 (ko) | 발효된 해조류의 상등액을 유효성분으로 포함하는 혈액응고억제용 조성물 및 그 제조방법 | |
CN102174524A (zh) | 脉羊耳兰凝集素基因、蛋白及其应用 | |
CN102286110B (zh) | 酵母展示型聚半乳糖醛酸酶协同超声波处理提取紫菜多糖的方法 | |
KR100945717B1 (ko) | 고추 추출물을 발효공법에 의해 변형시킨 변형고추펙틴과 그 제조방법 및 용도 | |
Hennink et al. | Production, characterization and application of rhamnoGlacturonase | |
RU2101294C1 (ru) | Способ получения пектина из надземных частей амаранта | |
Mohd Rais et al. | Aspergillus niger ATCC 16404 Producing Pectinase Couple To Polysulfone/Pluronic Membrane Ultrafiltration For Momordica Charantia Juice Clarification | |
Auta et al. | Production of Pectinase by Bacillus Species Cultured on Parkia biglobosa Fruit Pulp |