TW202120113A - Use of Momordica cochinchinensis Spreng oil for the preparation of skin-improving composition capable of having anti-aging effects of moisturization, wrinkle reduction, tightness, smoothness, and the like to the skin - Google Patents

Abstract

This invention relates to the use of Momordica cochinchinensis Spreng oil for preparing a skin-improving composition. The aforementioned skin improvement is selected from the group consisting of: promoting skin cell collagen synthesis; promoting skin cell hyaluronic acid synthesis; promotion of transglutaminase-1 (TGM-1) expression of skin cells; and any combination thereof. The Momordica cochinchinensis Spreng oil of this invention can have anti-aging effects of moisturization, wrinkle reduction, tightness, smoothness, and the like to the skin.

Description

Use of Momordica cochinchinensis oil for preparing skin-improving composition

The present invention provides a use of Momordica cochinchinensis Spreng oil for the preparation of a skin-improving composition, in particular a Momordica cochinchinensis Spreng oil for the preparation of enhancing collagen synthesis, promoting hyaluronic acid synthesis and promoting gluten transfer The use of transglutaminase-1 (Transglutaminase-1, TGM-1) composition.

The skin is the largest organ of the human body, which prevents damage from the external environment, such as ultraviolet rays, pathogenic bacteria, and external force damage. The skin also has the functions of preventing moisture loss and keeping warm. The skin is divided into epidermis, dermis and subcutaneous tissue from the outside to the inside.

The skin is rich in extracellular matrix (ECM) such as collagen and hyaluronic acid (commonly known as hyaluronic acid), which gives the skin elasticity, moisture retention, surface luster, and structure to support the skin. However, with age and the influence of the external environment, the ability of skin cells to secrete extracellular matrix decreases. At the same time, these extracellular matrix are degraded by collagenase and hyaluronidase (Hyaluronidase, HYAL), resulting in collagen and Loss of hyaluronic acid, damage to the skin barrier and loose structure, resulting in skin aging such as wrinkles, depressions, sagging, thinning, dryness, reduced water retention, fragility and irritation.

In recent years, people have paid more and more attention to skin care issues. There are many types of products, and some skin care products contain synthetic chemical substances. These ingredients may irritate the skin and even cause damage to health after long-term use. In addition, there are some oral or smeared collagen and hyaluronic acid products. However, oral supplementation of collagen or hyaluronic acid will be used by the body after being broken down and absorbed by the digestive tract, and will not be completely stored in the skin. Its effect Limited; the collagen or hyaluronic acid applied to the skin is difficult to be absorbed by the skin due to its too large molecular weight, and it is not easy to stay on the skin. Based on the above problems, it is really necessary to develop effective, natural and safe raw materials for skin-improving products.

The previous patent document KR 20150025080A discloses a composition containing the cockleshell fruit extract, which has the effect of activating PPARα/γ and can improve or inhibit skin aging. However, it only discloses that the cockleshell fruit extract has the first inhibitory effect on human fibroblasts. The synthesis of type collagenase (that is, the effect of inhibiting the decomposition of collagen). There is no prior literature that discloses the technical means of using Momordica chinensis fruit oil to prepare a composition that promotes collagen synthesis, promotes hyaluronic acid synthesis, and promotes TGM-1 expression.

All parts of the cochinchinensis fruit are rich in nutrients and effective ingredients, especially the aril has the highest content, which is rich in carotenoid, alpha-tocopherol, polyphenols, and flavonoids Compounds (flavonoids) and essential fatty acids, and the nutrients of the cochinchinensis are easier to be absorbed by the human body than other fruits and vegetables. Therefore, the research team of the present invention believes that the cochinchinensis has the potential to be developed as a skin-improving product.

In view of the above-mentioned problems, the present invention aims to provide a cochinchinensis fruit oil for preparing The use of the skin-improving composition, wherein the aforementioned skin-improving system is selected from the group consisting of promoting skin cell collagen synthesis, promoting skin cell hyaluronic acid synthesis, promoting skin cell transglutaminase-1 (TGM-1) performance and A group composed of any combination.

In order to achieve the foregoing objective, the present invention provides the following technical means.

The present invention provides a use of Momordica charantia oil for preparing a skin-improving composition, which is characterized in that the aforementioned skin-improving system is selected from the group consisting of: (1) promoting skin cell collagen synthesis; (2) promoting skin cells Hyaluronic acid synthesis; (3) Promote skin cell transglutaminase-1 (Transglutaminase-1, TGM-1) performance; and any combination thereof.

The Gac oil of the present invention is prepared by squeezing the arils of the Momordica cochinchinensis Spreng.

The use of the present invention for preparing a skin-improving composition is characterized in that the aforementioned skin-improving system is selected from the group consisting of: (1) promoting skin cell collagen synthesis; (2) promoting skin cell hyaluronic acid synthesis; 3) Promote the expression of skin cell transglutaminase-1 (TGM-1); and any combination thereof; wherein the concentration of the cochinchinensis oil in the aforementioned composition is 0.01-100μg/mL, preferably 0.01-50μg /mL, more preferably 0.01 to 1 μg/mL.

In an embodiment of the present invention, the aforementioned composition is used to promote skin cell collagen synthesis, and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01-100 μg/mL.

In an embodiment of the present invention, the aforementioned composition is used to promote the synthesis of hyaluronic acid in skin cells, and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01-100 μg/mL, preferably 0.01-50 μg/mL.

In an embodiment of the present invention, the aforementioned composition is used to promote the synthesis of intracellular hyaluronic acid in skin cells, and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01-50 μg/mL. In another embodiment of the present invention, the aforementioned composition can be used to promote the secretion of hyaluronic acid from skin cells, and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01-100 μg/mL.

In an embodiment of the present invention, the aforementioned promoting skin cell hyaluronic acid synthesis is selected from the group consisting of: promoting skin cell hyaluronic acid synthase (Hyaluronan synthase, HAS) performance; inhibiting hyaluronidase (Hyaluronidase) , HYAL) performance; and any combination thereof.

In an embodiment of the present invention, the aforementioned composition is used to promote the expression of skin cell hyaluronic acid synthase (HAS), wherein the aforementioned hyaluronic acid synthase (HAS) is hyaluronic acid synthase-2 (HAS) -2), and the concentration of the Momordica chinensis fruit oil in the aforementioned composition is 0.01-100 μg/mL, preferably 0.01-50 μg/mL, and more preferably 0.01-0.1 μg/mL.

In an embodiment of the present invention, the aforementioned composition is used to inhibit the expression of skin cell hyaluronidase (HYAL), wherein the aforementioned hyaluronidase (HYAL) is hyaluronidase-1 (HYAL-1), Hyaluronidase-2 (HYAL-2) or Hyaluronidase-3 (HYAL-3), and the concentration of cochinchinensis oil in the aforementioned composition is 0.01-100μg/mL, preferably 0.01-50μg/mL , And more preferably 0.01 to 0.1 μg/mL.

In an embodiment of the present invention, the aforementioned composition is used to promote the expression of skin cell transglutaminase-1 (TGM-1), and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01~1μg/mL .

The present invention is used for the above purposes, wherein the aforementioned skin cell line is the keratinocyte of the epidermal layer of human skin.

The present invention provides a use of Momordica charantia oil for preparing a skin-improving composition, so as to promote the synthesis of collagen by skin cells, promote the synthesis of hyaluronic acid by skin cells, promote the expression of TGM-1 protein of skin cells, and any combination thereof. Collagen and hyaluronic acid have the functions of moisturizing and wrinkle removal. TGM-1 can promote the arrangement of skin cells without cracks, so that skin moisture is not easily lost or evaporated from the body surface. Therefore, the skin-improving composition prepared by the present invention , It has anti-aging effects such as moisturizing, repairing wrinkles, firming and smoothing the skin.

[Fig. 1] A bar graph showing the effect of the Momordica charantia oil in Example 2 of the present invention on enhancing the synthesis of Type I procollagen in human skin keratinocytes (-, marked as the blank control group).

[Fig. 2A] A bar graph showing the effect of the Momordica charantia oil in Example 3 of the present invention on the promotion of intracellular hyaluronic acid synthesis in human skin keratinocytes (-, marked as the blank control group).

[Figure 2B] A bar graph of the effect of the Momordica charantia oil in Example 3 of the present invention on promoting the secretion of hyaluronic acid from human skin keratinocytes (-, marked as the blank control group).

[Figure 3] The Western blot method results and bar graphs of the Momordica charantia oil in Example 3 of the present invention for enhancing the expression of hyaluronic acid synthase-2 (HAS-2) protein (-, marked as the blank control group) .

[Figure 4] The Western blot method results and bar graphs (-, marked as the blank control group) for the inhibition of hyaluronidase-1 (HYAL-1) protein expression by the Momordica chinensis fruit oil of Example 3 of the present invention.

[Figure 5] A bar graph showing the effect of the Momordica charantia oil in Example 3 of the present invention on the expression of the hyaluronidase-2 (HYAL-2) gene.

[Figure 6] A bar graph showing the effect of the Momordica charantia oil in Example 3 of the present invention on the expression of the hyaluronidase-3 (HYAL-3) gene.

[Figure 7] The Western blot method results and bar graphs (-, marked as the blank control group) of the Momordica chinensis fruit oil in Example 4 of the present invention for enhancing the expression of transglutaminase-1 (TGM-1) protein ).

Next, specific embodiments of the present invention will be described, but the present invention is not particularly limited to these embodiments.

Example 1 Preparation method of Gac oil of the present invention

The Gac oil of the present invention is prepared by squeezing the arils of the Momordica cochinchinensis Spreng. Firstly, the fresh cockleshell fruit is cut open, the cockleshell fruit seeds are taken out, and the aril is peeled off; then, it is dried at 60°C for 24 to 48 hours; and roasted in a roasting pan at 80°C for 10 minutes. Finally, an industrial-grade screw extruder is used to squeeze out the oil, and the supernatant is centrifuged to obtain the Momordica charantia oil used in the preparation of the skin-improving composition of the present invention.

However, in the preparation method of Example 1 of the present invention, those with ordinary knowledge in the field can also perform reduced pressure concentration of the cochinchinensis fruit oil with a vacuum concentrator to further remove the residual moisture and improve the cochinchinensis fruit oil. concentration. When preparing the aforementioned skin-improving composition, a person with ordinary knowledge in the field can appropriately adjust the concentration of the cochineal fruit oil in the composition according to the aforementioned concentration step.

Example 2 The Gac oil of the present invention enhances the effect of keratinocytes in the skin to synthesize Type I procollagen

The following Examples 2 to 4 used HaCaT cells, a cell line of keratinocytes in the epidermal layer of human skin, for analysis.

It is known that collagen is the protein with the highest content in the human body. In skin tissues, both the epidermal layer and the dermis layer contain a large amount of collagen, which is an important component of the extracellular matrix. It is produced and secreted by skin cells. Between cells. Collagen can maintain skin elasticity and has a high affinity with water molecules, so it has a good moisturizing function. Collagen accounts for about 70% to 80% of the dry weight of human skin, which can support the overall structure of the dermis and make the skin plump without wrinkles. Among them, Type I collagen (Type I collagen) accounts for the majority of human skin tissues, about 85%~90%; followed by Type III collagen (Type III collagen), about 10%-15%.

The collagen in the skin will decrease with age, bad habits or the influence of the external environment, resulting in a decrease in biosynthesis and an increase in decomposition, resulting in a gradual loss of collagen, resulting in the disintegration of the skin structure and the decrease in thickness, which ultimately leads to the formation of wrinkles , It loses the elasticity of the skin, so it is very important to supplement and restore the collagen content in the skin, which is very important for skin health, reducing wrinkles, anti-UV damage and anti-aging.

The type 1 collagen is derived from the synthesis of type 1 procollagen by cells. Therefore, the present invention is to detect the effect of cochineal fruit oil on the synthesis of type 1 procollagen by skin keratinocytes.

In Example 2 of the present invention, human skin keratinocytes were treated with cell culture media containing concentrations of 0.01, 0.1, 1, 10, 50, and 100 μg/mL cochinchinensis oil as an experiment. In the test group, the treatment of cell culture medium without addition of cochineal fruit oil was used as the blank control group. Skin keratinocytes were treated with cochineal fruit oil for 24 hours, and the cells were collected and broken. Enzyme-linked immunosorbent assay (Enzyme- Linked Immunosorbent Assay, ELISA) analyzes the concentration of type I procollagen in the cell.

The experimental results of the effect of the cochinchinensis oil of the present invention in enhancing the synthesis of type 1 procollagen in human skin keratinocytes are shown in Fig. 1. The blank control group without cochinchinensis oil treatment, the human skin keratinocytes The concentration of type I procollagen in human skin is less than 10ng/mL; the experimental group treated with a 0.01μg/mL cochinchinensis oil, the concentration of type I procollagen in human skin keratinocytes is also less than 10ng/mL; In the experimental group treated with cochinchinensis oil at a concentration of 0.1μg/mL and 1μg/mL, the concentration of type I procollagen in human skin keratinocytes was about 10-20ng/mL; at a concentration of 10μg/mL In the experimental group treated with Momordica charantia oil, the concentration of type I procollagen in human skin keratinocytes was about 30-40ng/mL; the experimental group treated with Momordica charantia oil at a concentration of 50μg/mL, human skin keratin The concentration of type I procollagen in the producing cells is at least 40ng/mL; and the experimental group treated with a concentration of 100μg/mL of Momordica charantia oil, the concentration of type I procollagen in the human skin keratinocytes is at least 10ng/mL .

Therefore, human skin keratinocytes treated with cochinchinensis oil at a concentration of 0.1~100μg/mL have the effect of stimulating the synthesis of type 1 procollagen, compared with the blank control group not treated with cochinchinensis oil. Especially at a concentration of 50μg/mL cochinchinensis fruit oil, the effect of promoting synthesis is better.

The foregoing results show that the cochinchinensis oil of the present invention has an excellent effect of promoting the synthesis of type 1 preprotein by human skin keratinocytes, and can effectively enhance the collagen in the skin To maintain the structural integrity and elasticity of the skin, and slow down the generation of wrinkles.

Example 3 The effect of the cochinchinensis fruit oil of the present invention in promoting the synthesis and secretion of hyaluronic acid in human skin keratinocytes

It is known that keratinocytes secrete hyaluronic acid and other substances as intercellular substance to maintain the integrity of the epidermal barrier, prevent skin water loss and form a complete protection. In addition, hyaluronic acid has a high affinity with water molecules, can hold moisture, and has a good moisturizing effect. Therefore, the effect of the Momordica charantia oil of the present invention in promoting the production of hyaluronic acid by skin keratinocytes was further tested.

Promote the production and secretion of hyaluronic acid

This example intends to test the effect of Momordica charantia oil in promoting the synthesis of hyaluronic acid in human skin keratinocytes. Cell culture media with concentrations of 0.01, 0.1, 1, 10, and 50 μg/mL Momordica charantia oil were used to treat human skin keratinogenesis. The cells were used as the experimental group, and the treatment of cell culture medium without the cochinchinensis oil was used as the blank control group. After the human skin keratinocytes were treated with cochinchinensis oil for 24 hours, the cells were collected and broken, and the enzyme-linked immunosorbent assay was used. Method (Enzyme-Linked Immunosorbent Assay, ELISA) to analyze the intracellular hyaluronic acid concentration.

The results of the experiment are shown in Figure 2A. Under the baseline correction of the values measured in the blank control group that was not treated with the cochinchinensis oil, the experimental group treated with the cochinchinensis oil at a concentration of 0.01μg/mL showed human skin keratinocytes. The concentration of intracellular hyaluronic acid increased by about 0~10ng/mL; in the experimental group treated with cochinchinensis oil at a concentration of 0.1μg/mL, the concentration of intracellular hyaluronic acid increased by at least 10ng/mL; respectively, with a concentration of 1μg/mL. The concentration of intracellular hyaluronic acid increased by about 10-20 ng/mL in the experimental groups treated with mL and 10μg/mL cochinchinensis oil; the experimental group treated with cochinchinensis oil at a concentration of 50μg/mL showed intracellular hyalurin The concentration of acid increases by about 20~30ng/mL.

This result shows that the cochinchinensis oil of the present invention has the effect of enhancing the intracellular hyaluronic acid synthesis of human skin keratinocytes at an action concentration of 0.01-50μg/mL, and the effect is better at an action concentration of 50μg/mL .

In order to further test the effect of the cochinchinensis oil of the present invention in promoting the secretion of hyaluronic acid from skin keratinocytes, human skin was treated with a cell culture medium containing 0.01, 0.1, 1, 10, 50 and 100μg/mL cochinchinensis oil. Keratinocytes were used as the experimental group, and the treatment of cell culture medium without the cochinchinensis oil was used as the blank control group. After the human skin keratinocytes were treated with cochinchinensis oil for 24 hours, the cell culture medium without cells was collected. Enzyme-Linked Immunosorbent Assay (ELISA) analyzes the concentration of hyaluronic acid secreted to the outside of the cell.

The results of the experiment are shown in Figure 2B. Under baseline correction with the values measured in the blank control group that has not been treated with the cochinchinensis oil, the experimental group treated with the cochinchinensis oil at a concentration of 0.01μg/mL secretes to the outside of the cell. The concentration of hyaluronic acid increased by about 5ng/mL; in the experimental group treated with cochinchinensis oil at concentrations of 0.1, 1, 10, and 50μg/mL, the concentration of hyaluronic acid secreted to the outside of the cell increased by about 5-10ng/mL ; In the experimental group treated with the cochinchinensis oil at a concentration of 100μg/mL, the concentration of hyaluronic acid secreted to the outside of the cell increased by at least 10ng/mL.

This result shows that the cochinchinensis fruit oil of the present invention has the effect of enhancing the secretion of hyaluronic acid by human skin keratinocytes at an action concentration of 0.01-100 μg/mL, and the effect is better at an action concentration of 100 μg/mL.

Promote the performance of hyaluronic acid synthase (HAS)

It is known that the promotion of hyaluronic acid synthase (HAS) can promote the ability of skin cells to secrete hyaluronic acid, complete the structure of the skin stratum corneum and enhance the skin barrier function, which can make the skin water-retaining Power increase.

Human skin keratinocytes were treated with cell culture medium containing 0.01μg/mL and 0.1μg/mL cochinchinensis oil respectively as the experimental group, while the treatment of cell culture medium without cochinchinensis oil was used as the blank control group. After the skin keratinocytes were treated with Momordica charantia oil for 24 hours, the cells were collected and broken, the intracellular proteins were extracted, and the Western blot method was performed with the HAS-2 specific antibody to analyze the protein expression of HAS-2 in the keratinocytes. .

The experimental results of the Momordica charantia oil of the present invention for enhancing the performance of HAS-2 protein are shown in Fig. 3. After the human skin keratinocytes are treated with the Momordica charantia oil at a concentration of 0.01 μg/mL and 0.1 μg/mL, compared with In the blank control group without the addition of cochinchinensis oil, the expression of HAS-2 protein in human skin keratinocytes increased to between 1.0 and 1.5 times.

Inhibit the performance of hyaluronidase (HYAL)

It is known that hyaluronidase (HYAL) is an enzyme that degrades hyaluronic acid, disintegrates the structure of hyaluronic acid and loses its original function.

Human skin keratinocytes were treated with cell culture media containing 0.01, 0.1, 1, 10, 50, and 100 μg/mL cochinchin oil as the experimental group, and the cell culture medium without cochinchin oil was used as the blank In the control group, human skin keratinocytes were treated with Momordica charantia oil for 24 hours, the cells were collected and broken, the intracellular proteins were extracted, and the Western blot method was performed with HYAL-1 specific antibody to analyze the HYAL-1 in the keratinocytes. The amount of protein expression.

The experimental results of the Momordica charantia oil of the present invention for inhibiting the expression of HYAL-1 protein are shown in Fig. 4. Compared with the blank control group that was not treated with Momordica charantia oil, it was treated with Momordica charantia oil at a concentration of 0.01 μg/mL. In the experimental group, the expression level of HYAL-1 protein in human skin keratinocytes Decreased to between 0.6 and 0.8 times; in the experimental group treated with cochineal fruit oil at a concentration of 0.1 μg/mL and 1 μg/mL, the expression of HYAL-1 protein in keratinocytes was reduced to between 0.4 and 0.6 times; respectively; In the experimental groups treated with cochinchinensis oil at concentrations of 10, 50 and 100 μg/mL, the expression of HYAL-1 protein in human skin keratinocytes was reduced to less than about 0.2 times. This result shows that the cochinchinensis oil of the present invention has the effect of inhibiting the expression of HYAL-1 protein in human skin keratinocytes at an action concentration of 0.01~100μg/mL, and when the action concentration is 10μg/mL, it can inhibit the expression of HYAL-1 protein in human skin keratinocytes. The effect is better.

In order to further test the effect of the cochinchinensis oil of the present invention on inhibiting the expression of HYAL-2 and HYAL-3 genes, human skin keratinocytes were treated with a cell culture medium containing 0.1μg/mL cochinchinensis oil as the experimental group. At the same time Take the treatment of cell culture medium without addition of Momordica charantia oil as the blank control group. After the human skin keratinocytes are treated with Momordica charantia oil for 24 hours, the cells are collected and broken, and the RNA in the keratinocytes is extracted. Combine primers for real-time quantitative reverse transcription polymerase chain reaction (Real-time quantitative reverse transcription polymerase chain reaction) experiments to analyze the mRNA expression levels of HYAL-2 and HYAL-3 genes in keratinocytes. The experimental results are obtained through three independent experiments. The experimental data are expressed as mean±standard deviation, and the blank control group is used as the benchmark (that is, the gene mRNA expression level of the blank control group is set to 1.0; *, means compared to the blank control group p<0.05; **, means p<0.01 compared to the blank control group).

The experimental results of the Momordica charantia oil of the present invention on inhibiting the expression of HYAL-2 and HYAL-3 genes are shown in Fig. 5 and Fig. 6. After the human skin keratinocytes are treated with 0.1μg/mL Momordica charantia oil, compared with In the blank control group that was not treated with cochineal fruit oil, the gene expression levels of HYAL-2 and HYAL-3 were both reduced to between 0.5 and 1.0 times. This result shows that the cochinchinensis oil of the present invention can significantly inhibit the gene expression of HYAL-2 and HYAL-3 in human skin keratinocytes at an action concentration of 0.1 μg/mL.

In summary, the cochinchinensis oil of the present invention has excellent effects of promoting HAS-2 and inhibiting the performance of HYAL-1, HYAL-2 and HYAL-3, thereby promoting the synthesis of human skin keratinocytes and the ability to secrete hyaluronic acid , It also has the effect of inhibiting the degradation of hyaluronic acid, which can effectively complete the structure of the skin stratum corneum and enhance the skin barrier function, and enhance the skin's moisture retention.

Example 4 The cochinchinensis fruit oil of the present invention enhances the expression level of transglutaminase-1 (TGM-1) in skin keratinocytes

Previous studies have pointed out that transglutaminase-1 (TGM-1) will form a strong bond between the cell membrane of keratinocytes and structural proteins, increase the strength and stability of the epidermis, and promote the orderly arrangement of skin cells. Cracks are produced, making the skin's moisture less likely to be lost from the body surface, It evaporates, so it has the functions of moisturizing, making the skin healthier and smoother.

Human skin keratinocytes were treated with cell culture medium containing 0.01, 0.1, and 1μg/mL cochinchin oil as the experimental group, and the cell culture medium without cochinchin oil was used as the blank control group. Human skin keratinocytes After 24 hours of treatment of the producing cells with the cochinchinensis oil, the cells were collected and broken, and the intracellular proteins were extracted. The western blotting experiment was carried out with the TGM-1 specific antibody to analyze the protein expression of TGM-1 in the keratinocytes.

The experimental results of the Momordica charantia oil of the present invention for enhancing the expression of TGM-1 protein in human skin keratinocytes are shown in Fig. 7. Compared with the blank control group that has not been treated with Momordica charantia oil, the concentration is 0.01 μg/mL. In the experimental group treated with the cochinchinensis oil, the expression of TGM-1 protein in human skin keratinocytes increased to between 1.0 and 1.5 times; the experiment was treated with the cochinchinensis oil at a concentration of 0.1μg/mL and 1μg/mL, respectively In the group, the expression level of TGM-1 protein in human skin keratinocytes increased to between 1.5 and 2.0 times.

This result shows that the cochinchinensis oil of the present invention can enhance the protein expression of TGM-1 in human skin keratinocytes at an action concentration of 0.01~1μg/mL, and at an action concentration of 1μg/mL, its effect is better, Make the skin stratum corneum structure complete and improve skin moisture retention.

【Industrial Applicability】

In summary, the cochinchinensis oil of the present invention can effectively enhance the production of collagen in human skin keratinocytes, restore smoothness, firmness and elasticity to the skin, and reduce the generation of fine lines and wrinkles; by enhancing HAS-2 and inhibiting HYAL- 1. The protein expression of HYAL-2 and HYAL-3 in keratinocytes promotes the synthesis of human skin keratinocytes and the secretion of hyaluronic acid, improves skin moisturizing ability and elasticity; and promotes TGM-1 in keratinogenesis Egg in cell White expression, maintain the neat arrangement of skin keratinocytes, make the skin structure intact, and improve the skin barrier function and water retention. Therefore, the Momordica charantia oil of the present invention can be used for the preparation of skin-improving compositions, achieving skin moisturizing, reducing fine lines, wrinkles, smoothing, firming, elasticity and other anti-aging effects.

<110> National Pingtung University of Science and Technology

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Claims (9)

A use of Momordica cochinchinensis oil for the preparation of a skin-improving composition, characterized in that the aforementioned skin-improving system is selected from the group consisting of: (1) promoting skin cell collagen synthesis; (2) promoting skin Cellular hyaluronic acid synthesis; (3) Promote skin cell transglutaminase-1 (Transglutaminase-1, TGM-1) performance; and any combination thereof. As the use described in item 1 of the scope of the patent application, the concentration of the cochineal fruit oil in the aforementioned composition is 0.01-100 μg/mL. The use described in item 1 of the scope of patent application, wherein the aforementioned composition is used to promote the synthesis of skin cell collagen, and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01-100 μg/mL. The use described in item 1 of the scope of the patent application, wherein the aforementioned composition is used to promote the synthesis of hyaluronic acid in skin cells, and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01-100 μg/mL. The use described in item 1 of the scope of patent application, wherein the aforementioned composition is used to promote the expression of skin cell transglutaminase-1 (TGM-1), and the concentration of the cochinchinensis oil in the aforementioned composition is 0.01~1μg/mL. The use described in item 4 of the scope of patent application, wherein the aforementioned promotion of skin cell hyaluronic acid synthesis is selected from the group consisting of: promoting skin cell hyaluronic acid synthase (Hyaluronan synthase, HAS) performance; inhibiting skin The performance of cellular hyaluronidase (Hyaluronidase, HYAL); and any combination thereof. Such as the use described in item 6 of the scope of patent application, wherein the aforementioned composition is used to promote skin The performance of skin cell hyaluronic acid synthase (HAS), the aforementioned hyaluronic acid synthase (HAS) is hyaluronic acid synthase-2 (HAS-2), and the concentration of the mochi oil in the aforementioned composition is 0.01 ~100μg/mL. As stated in item 6 of the scope of patent application, the aforementioned composition is used to inhibit the expression of skin cell hyaluronidase (HYAL), and the aforementioned hyaluronidase (HYAL) is hyaluronidase-1 (HYAL). -1), Hyaluronidase-2 (HYAL-2) or Hyaluronidase-3 (HYAL-3), and the concentration of Momordica charantia oil in the aforementioned composition is 0.01-100 μg/mL. The use described in any one of items 1 to 8 in the scope of the patent application, wherein the aforementioned skin cell line is the keratinocyte of the epidermal layer of human skin.

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