TW200704778A - Screening using polarization anisotropy in fret emissions - Google Patents

Screening using polarization anisotropy in fret emissions

Info

Publication number
TW200704778A
TW200704778A TW095111992A TW95111992A TW200704778A TW 200704778 A TW200704778 A TW 200704778A TW 095111992 A TW095111992 A TW 095111992A TW 95111992 A TW95111992 A TW 95111992A TW 200704778 A TW200704778 A TW 200704778A
Authority
TW
Taiwan
Prior art keywords
fluorophore
chemical entity
chemical
fret
entities
Prior art date
Application number
TW095111992A
Other languages
English (en)
Chinese (zh)
Inventor
Steven C Miller
Paul B Comita
Christopher B Shumate
Evan F Cromwell
Original Assignee
Blueshift Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Blueshift Biotechnologies Inc filed Critical Blueshift Biotechnologies Inc
Publication of TW200704778A publication Critical patent/TW200704778A/zh

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
TW095111992A 2005-04-04 2006-04-04 Screening using polarization anisotropy in fret emissions TW200704778A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66842805P 2005-04-04 2005-04-04
US67884205P 2005-05-06 2005-05-06

Publications (1)

Publication Number Publication Date
TW200704778A true TW200704778A (en) 2007-02-01

Family

ID=37073796

Family Applications (1)

Application Number Title Priority Date Filing Date
TW095111992A TW200704778A (en) 2005-04-04 2006-04-04 Screening using polarization anisotropy in fret emissions

Country Status (5)

Country Link
US (2) US20060234279A1 (https=)
EP (1) EP1866443A4 (https=)
JP (1) JP4921458B2 (https=)
TW (1) TW200704778A (https=)
WO (1) WO2006107864A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102548466A (zh) * 2009-07-28 2012-07-04 霍夫曼-拉罗奇有限公司 非侵入性体内光学成像方法
TWI808554B (zh) * 2020-12-04 2023-07-11 亞光股份有限公司 使用陰極發光測量判別半導體材料中的位錯類型和密度的裝置與方法
US11782001B2 (en) 2020-12-04 2023-10-10 Attolight AG Dislocation type and density discrimination in semiconductor materials using cathodoluminescence measurements

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4921458B2 (ja) * 2005-04-04 2012-04-25 ブルーシフト・バイオテクノロジーズ・インコーポレーテッド Ｆｒｅｔ発光における偏光異方性を使用したスクリーニングの方法
JP5189301B2 (ja) * 2007-03-12 2013-04-24 オリンパス株式会社 レーザー走査型顕微鏡
ES2462759T3 (es) 2008-10-01 2014-05-26 Sherwin Hua Sistema para estabilización de tornillo pedicular guiado por alambre de vertebras de la columna
US8568306B2 (en) 2009-04-03 2013-10-29 Mitchell A Hardenbrook Surgical retractor system
CN102859997B (zh) 2010-03-26 2018-05-04 艾科星科技公司 多输入电视接收器
EP3560445A1 (en) 2010-03-30 2019-10-30 Sherwin Hua Systems for pedicle screw stabilization of spinal vertebrae
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
US20140099264A1 (en) 2011-03-07 2014-04-10 F. Hoffman-La Roche Ag Means and methods for in vivo testing of therapeutic antibodies
JP6385060B2 (ja) 2011-03-07 2018-09-05 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 治療的に活性な抗体のインビボにおける選択
KR101167649B1 (ko) 2011-04-20 2012-07-20 한국과학기술원 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 장치
US9746470B2 (en) * 2011-05-09 2017-08-29 Whitehead Institute For Biomedical Research Chaperone interaction assays and uses thereof
JP5877741B2 (ja) * 2012-03-16 2016-03-08 株式会社日立製作所 細胞数モニタリング方法
WO2014059281A1 (en) * 2012-10-11 2014-04-17 The Regents Of The University Of Michigan Fluorescence polarization assay for detecting high molecular weight molecules in biological fluids
CN102967554B (zh) * 2012-10-29 2016-12-21 广东工业大学 一种双通道、单光路结构的荧光各向异性显微成像装置及方法
LT3060677T (lt) * 2013-10-25 2018-08-27 Biomadison, Inc. Fermentų tyrimo su dublikuotais fluoroforais būdas
ES2958398T3 (es) 2015-01-23 2024-02-08 Bayer Ag Procedimiento y dispositivo para determinar el efecto de sustancias activas sobre nematodos y otros organismos en ensayos acuosos
CN109509509B (zh) * 2018-09-29 2020-12-22 江西理工大学 基于动态加权蛋白质相互作用网络的蛋白质复合物挖掘方法
US11160580B2 (en) 2019-04-24 2021-11-02 Spine23 Inc. Systems and methods for pedicle screw stabilization of spinal vertebrae
EP4065016B1 (en) 2019-11-27 2025-10-22 Spine23 Inc. Systems for treating a lateral curvature of a spine
US12076058B2 (en) 2021-05-12 2024-09-03 Spine23 Inc. Systems and methods for pedicle screw stabilization of spinal vertebrae

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5524405A (en) 1994-02-28 1996-06-11 Byrd; Randall Wall structure
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
US6573045B1 (en) * 1998-06-05 2003-06-03 Ribotargets, Ltd. Methods and kits for discovery of RNA-binding compounds
EP1188057B1 (en) * 1999-06-24 2008-01-23 McMASTER UNIVERSITY Incorporation and applications of biomolecular interactions within a carrier
US6509161B1 (en) * 2000-02-29 2003-01-21 Gentronix Limited Green fluorescent protein
US6777184B2 (en) * 2000-05-12 2004-08-17 Caliper Life Sciences, Inc. Detection of nucleic acid hybridization by fluorescence polarization
EP1409982A4 (en) * 2001-06-14 2006-05-24 Anadys Pharmaceuticals Inc PROCESS FOR SCREENING ON LIGANDS OF TARGET MOLECULES
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
US20030228703A1 (en) 2002-04-05 2003-12-11 The Regents Of The University Of Michigan Fluorescence resonance energy transfer quantitation and stoichiometry in living cells
US6713262B2 (en) * 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
JP2004093527A (ja) * 2002-09-04 2004-03-25 Center For Advanced Science & Technology Incubation Ltd 蛍光共鳴エネルギー転移を用いた蛋白質の分析方法
JP4214206B2 (ja) * 2002-12-10 2009-01-28 独立行政法人理化学研究所 Ｆｒｅｔを利用した蛍光指示薬
JP2007504445A (ja) 2003-08-26 2007-03-01 ブルーシフト・バイオテクノロジーズ・インコーポレーテッド 時間依存蛍光計測
US20050264805A1 (en) 2004-02-09 2005-12-01 Blueshift Biotechnologies, Inc. Methods and apparatus for scanning small sample volumes
FR2872287B1 (fr) * 2004-06-28 2007-03-16 Cis Bio Internat Sa Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif
JP2008505321A (ja) * 2004-07-02 2008-02-21 ブルーシフト・バイオテクノロジーズ・インコーポレーテッド 蛍光体微小環境の探索
US8031338B2 (en) * 2004-12-02 2011-10-04 Vanderbilt University Measuring Forster resonance energy transfer with polarized and depolarized light
JP4921458B2 (ja) * 2005-04-04 2012-04-25 ブルーシフト・バイオテクノロジーズ・インコーポレーテッド Ｆｒｅｔ発光における偏光異方性を使用したスクリーニングの方法
JP5189301B2 (ja) * 2007-03-12 2013-04-24 オリンパス株式会社 レーザー走査型顕微鏡

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102548466A (zh) * 2009-07-28 2012-07-04 霍夫曼-拉罗奇有限公司 非侵入性体内光学成像方法
TWI808554B (zh) * 2020-12-04 2023-07-11 亞光股份有限公司 使用陰極發光測量判別半導體材料中的位錯類型和密度的裝置與方法
US11782001B2 (en) 2020-12-04 2023-10-10 Attolight AG Dislocation type and density discrimination in semiconductor materials using cathodoluminescence measurements

Also Published As

Publication number Publication date
WO2006107864A1 (en) 2006-10-12
JP4921458B2 (ja) 2012-04-25
EP1866443A4 (en) 2009-04-08
US20080206888A1 (en) 2008-08-28
US20060234279A1 (en) 2006-10-19
US7674588B2 (en) 2010-03-09
JP2008538004A (ja) 2008-10-02
EP1866443A1 (en) 2007-12-19

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