SV2002000438A - Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado ref. lea 33419-sv - Google Patents
Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado ref. lea 33419-svInfo
- Publication number
- SV2002000438A SV2002000438A SV2001000438A SV2001000438A SV2002000438A SV 2002000438 A SV2002000438 A SV 2002000438A SV 2001000438 A SV2001000438 A SV 2001000438A SV 2001000438 A SV2001000438 A SV 2001000438A SV 2002000438 A SV2002000438 A SV 2002000438A
- Authority
- SV
- El Salvador
- Prior art keywords
- inclusion bodies
- protein inclusion
- purification
- suspension
- separation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
LA INVENCION SE REFIERE A UN PROCEDIMIENTO PARA LA PURIFICACION Y/O CONCENTRACION DE CUERPOS DE INCLUSION DE PROTEINAS (EN INGLES "INCLUSION BODIES" O "REFLACTILE BODIES")EN UNA SOLUCION ALTAMENTE CONCENTRADA QUE CONTIENE PARTICULAS, EN EL QUE LA SUSPENSION QUE CONTIENE LOS CUERPOS DE INCLUSION DE PROTEINAS SE HACE PASAR TANGENCIALMENTE A UNA O VARIAS MEMBRANAS SEMIPERMEABLES, DE MODO QUE LOS CUERPOS DE INCLUSION DE PROTEINAS QUEDAN RECHAZADOS DETRAS DE LAS MEMBRANAS Y LAS SUBSTANCIAS DE MENOR PESO MOLECULAR PUEDEN PASAR A TRAVES DE LA MEMBRANA Y/O QUEDAN ABSORBIDAS EN LA MEMBRANA Y SE OBTIENE UNA SUSPENSION DE CUERPOS DE INCLUSION DE PROTEINAS PURIFICADA Y/O CONCENTRADA. DEL MISMO MODO SE PROCEDE PARA LA SEPARACION DE PARTICULAS DE PARED CELULAR DE UNA SUSPENSION DE CUERPOS DE INCLUSION DE PROTEINAS. ADEMAS, LA INVENCION SE REFIERE AL USO DE UNA INSTALACION DE MICROFILTRACION DE FLUJO CRUZADO PARA LA PURIFICACION Y/O CONCENTRACION DE CUERPOS DE INCLUSION DE PROTEINAS EN UNA SUSPENSION ASI COMO PARA LA SEPARACION DE PARTICULAS DE PARED CELULAR DE UNA SUSPENSION DE CUERPOS DE INCLUSION DE PROTEINAS, ADEMAS LA INVENCION SE REFIERE AL USO DE UNA INSTALACION DE MICCROFILTRACION DE FLUJO CRUZADO PARA LA PURIFICACION Y/O CONCENTRACION DE CUERPOS DE INCLUSION DE PROTEINAS EN UNA SUSPENSION ASI COMO PARA LA SEPARACION DE PARTICULAS DE PARED CELULAR DE UNA PREPARACION DE CUERPOS DE INCLUSION DE PROTEINAS.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10022258A DE10022258A1 (de) | 2000-05-08 | 2000-05-08 | Reinigung von Proteineinschlusskörpern durch Querstrom-Mikrofiltration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SV2002000438A true SV2002000438A (es) | 2002-07-03 |
Family
ID=7641110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SV2001000438A SV2002000438A (es) | 2000-05-08 | 2001-05-08 | Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado ref. lea 33419-sv |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6936699B2 (es) |
| EP (1) | EP1282637B1 (es) |
| JP (1) | JP4818561B2 (es) |
| AR (1) | AR028392A1 (es) |
| AU (1) | AU2001272378A1 (es) |
| CA (1) | CA2408902C (es) |
| DE (2) | DE10022258A1 (es) |
| DO (1) | DOP2001000159A (es) |
| ES (1) | ES2215916T3 (es) |
| MY (1) | MY126838A (es) |
| PE (1) | PE20020003A1 (es) |
| SV (1) | SV2002000438A (es) |
| TW (1) | TWI248940B (es) |
| WO (1) | WO2001085757A2 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10237082B4 (de) * | 2002-08-09 | 2014-12-31 | Sartorius Stedim Biotech Gmbh | Verfahren und Vorrichtung zur biotechnologischen Herstellung von Wertstoffen |
| EP1718738A2 (en) * | 2004-02-23 | 2006-11-08 | Crucell Holland B.V. | Virus purification methods |
| DK1869171T4 (en) * | 2005-04-11 | 2016-01-18 | Crucell Holland Bv | Virus cleaning using ultrafiltration |
| RU2468373C2 (ru) | 2007-07-03 | 2012-11-27 | Эмджен Инк. | Количественное определение белков с использованием сухого веса внутриклеточных телец |
| US8535536B1 (en) | 2009-07-04 | 2013-09-17 | University Of Utah Research Foundation | Cross-flow split-thin-flow cell |
| CN103797053B (zh) | 2011-05-04 | 2017-06-20 | 康奈尔大学 | 多嵌段共聚物膜及其制备方法和用途 |
| WO2013082599A1 (en) * | 2011-12-01 | 2013-06-06 | Barofold, Inc. | Methods and systems for protein refolding |
| IN2013MU02416A (es) * | 2013-07-19 | 2015-06-19 | Biogenomics Ltd | |
| JP2015065942A (ja) * | 2013-09-30 | 2015-04-13 | 富士フイルム株式会社 | 細胞培養担体および細胞培養容器 |
| KR101663036B1 (ko) | 2014-07-11 | 2016-10-06 | 한국과학기술원 | 미세조류 농축 및 수소 생성 장치 |
| WO2016078667A1 (en) * | 2014-11-17 | 2016-05-26 | Syddansk Universitet | Assembly for assessing drug permeability with adjustable biomimetic properties |
| US11628409B2 (en) | 2016-04-28 | 2023-04-18 | Terapore Technologies, Inc. | Charged isoporous materials for electrostatic separations |
| JP7104040B2 (ja) | 2016-11-17 | 2022-07-20 | ケー． シースジ，ジェイラジ | 高分子量の親水性添加剤を含有するイソポーラス自己集合ブロックコポリマーフィルム及びその製造方法 |
| CA3054137C (en) * | 2017-02-22 | 2023-02-21 | Terapore Technologies, Inc. | Ligand bound mbp membranes, uses and method of manufacturing |
| CN110621394A (zh) | 2017-05-12 | 2019-12-27 | 特拉波雷技术有限公司 | 耐化学性氟化多嵌段聚合物结构、制造方法和用途 |
| MX2020009506A (es) | 2018-03-12 | 2021-01-15 | Terapore Tech Inc | Materiales de copolímero de bloques asimétricos mesoporosos isoporosos con macrohuecos y método para fabricarlo. |
| CN112452157B (zh) * | 2020-11-26 | 2023-05-26 | 济南磐升生物技术有限公司 | 一种胶原蛋白提取纯化装置 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4137333A1 (de) | 1991-11-13 | 1993-05-19 | W Prof Dr Sebald | Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung |
| US5616595A (en) * | 1995-06-07 | 1997-04-01 | Abbott Laboratories | Process for recovering water insoluble compounds from a fermentation broth |
| EP0853123A1 (de) * | 1997-01-10 | 1998-07-15 | Roche Diagnostics GmbH | Reinigung von DNA durch Cross-Flow-Filtration |
| EP1022337B1 (en) | 1999-01-20 | 2006-07-26 | Bayer HealthCare AG | Plasmids, their construction and their use in the manufacture of interleukin-4 and interleukin-4 muteins |
| DE69929638T2 (de) | 1999-01-20 | 2006-09-21 | Bayer Healthcare Ag | Plasmide, deren Konstruktion und Anwendung zur Herstellung von Interleukin-4 und dessen Muteine |
-
2000
- 2000-05-08 DE DE10022258A patent/DE10022258A1/de not_active Withdrawn
-
2001
- 2001-04-20 TW TW090109473A patent/TWI248940B/zh active
- 2001-04-25 DE DE50101661T patent/DE50101661D1/de not_active Expired - Lifetime
- 2001-04-25 ES ES01951459T patent/ES2215916T3/es not_active Expired - Lifetime
- 2001-04-25 CA CA2408902A patent/CA2408902C/en not_active Expired - Fee Related
- 2001-04-25 EP EP01951459A patent/EP1282637B1/de not_active Expired - Lifetime
- 2001-04-25 AU AU2001272378A patent/AU2001272378A1/en not_active Abandoned
- 2001-04-25 US US10/275,797 patent/US6936699B2/en not_active Expired - Fee Related
- 2001-04-25 JP JP2001582356A patent/JP4818561B2/ja not_active Expired - Fee Related
- 2001-04-25 WO PCT/EP2001/004634 patent/WO2001085757A2/de active IP Right Grant
- 2001-04-30 AR ARP010102037A patent/AR028392A1/es active IP Right Grant
- 2001-05-04 MY MYPI20012100 patent/MY126838A/en unknown
- 2001-05-04 DO DO2001000159A patent/DOP2001000159A/es unknown
- 2001-05-08 SV SV2001000438A patent/SV2002000438A/es not_active Application Discontinuation
- 2001-05-08 PE PE2001000410A patent/PE20020003A1/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003535582A (ja) | 2003-12-02 |
| EP1282637A2 (de) | 2003-02-12 |
| US6936699B2 (en) | 2005-08-30 |
| PE20020003A1 (es) | 2002-03-28 |
| TWI248940B (en) | 2006-02-11 |
| MY126838A (en) | 2006-10-31 |
| WO2001085757A2 (de) | 2001-11-15 |
| DOP2001000159A (es) | 2002-05-15 |
| AR028392A1 (es) | 2003-05-07 |
| WO2001085757A3 (de) | 2002-03-28 |
| DE10022258A1 (de) | 2001-11-15 |
| DE50101661D1 (de) | 2004-04-15 |
| CA2408902C (en) | 2010-09-07 |
| EP1282637B1 (de) | 2004-03-10 |
| ES2215916T3 (es) | 2004-10-16 |
| US20030171560A1 (en) | 2003-09-11 |
| JP4818561B2 (ja) | 2011-11-16 |
| CA2408902A1 (en) | 2002-11-05 |
| AU2001272378A1 (en) | 2001-11-20 |
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