SU1471949A3 - Способ конструировани рекомбинантной плазмидной ДНК, кодирующей бычий гормон роста - Google Patents

Способ конструировани рекомбинантной плазмидной ДНК, кодирующей бычий гормон роста Download PDF

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Publication number
SU1471949A3
SU1471949A3 SU813345603A SU3345603A SU1471949A3 SU 1471949 A3 SU1471949 A3 SU 1471949A3 SU 813345603 A SU813345603 A SU 813345603A SU 3345603 A SU3345603 A SU 3345603A SU 1471949 A3 SU1471949 A3 SU 1471949A3
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SU
USSR - Soviet Union
Prior art keywords
growth hormone
cdna
dna
pbr
fragment
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SU813345603A
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English (en)
Russian (ru)
Inventor
Л.Миллер Уолтер
А.Мартиал Джозеф
Д.Бакстер Джон
Original Assignee
Дзе Раджентс Оф Дзе Юниверсити Оф Колифорния (Фирма)
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Application filed by Дзе Раджентс Оф Дзе Юниверсити Оф Колифорния (Фирма) filed Critical Дзе Раджентс Оф Дзе Юниверсити Оф Колифорния (Фирма)
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Publication of SU1471949A3 publication Critical patent/SU1471949A3/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Fodder In General (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
SU813345603A 1980-08-26 1981-10-23 Способ конструировани рекомбинантной плазмидной ДНК, кодирующей бычий гормон роста SU1471949A3 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US18134880A 1980-08-26 1980-08-26

Publications (1)

Publication Number Publication Date
SU1471949A3 true SU1471949A3 (ru) 1989-04-07

Family

ID=22663908

Family Applications (1)

Application Number Title Priority Date Filing Date
SU813345603A SU1471949A3 (ru) 1980-08-26 1981-10-23 Способ конструировани рекомбинантной плазмидной ДНК, кодирующей бычий гормон роста

Country Status (24)

Country Link
EP (1) EP0047600B1 (https=)
JP (2) JP2534221B2 (https=)
KR (1) KR830007822A (https=)
AT (1) ATE72833T1 (https=)
AU (1) AU544453B2 (https=)
CA (1) CA1225948A (https=)
CZ (1) CZ280596B6 (https=)
DD (4) DD212403A5 (https=)
DE (1) DE3177273D1 (https=)
DK (1) DK372881A (https=)
ES (2) ES504968A0 (https=)
FI (1) FI812614L (https=)
GR (1) GR74350B (https=)
HU (1) HU195535B (https=)
IE (1) IE52755B1 (https=)
IL (1) IL63628A (https=)
NZ (1) NZ198050A (https=)
PH (2) PH21216A (https=)
PL (1) PL232797A1 (https=)
PT (1) PT73569B (https=)
RO (1) RO105533B1 (https=)
SU (1) SU1471949A3 (https=)
YU (1) YU44962B (https=)
ZA (1) ZA815696B (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4443539A (en) * 1980-02-05 1984-04-17 The Upjohn Company Process for preparing bovine growth hormone
JPS58996A (ja) * 1981-06-01 1983-01-06 ザ・ボ−ド・オブ・トラステイ−ズ・ミシガンステ−トユニバ−シテイ− 細菌中での牛成長ホルモン遺伝子のクロ−ン化
NZ201918A (en) * 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
EP0278121B1 (en) * 1981-09-18 1995-09-06 Genentech, Inc. Microbially produced bovine growth hormone (BGH) and its use
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
IE55244B1 (en) * 1982-05-25 1990-07-18 Lilly Co Eli Cloning vectors for expression of exogenous protein
EP0103395A3 (en) * 1982-08-17 1985-05-22 Biogen N.V. Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield
WO1984001150A1 (en) * 1982-09-16 1984-03-29 Amgen Avian growth hormones
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
ZA837173B (en) * 1982-11-08 1984-05-30 Univ Case Western Reserve Genomic bovine growth hormone
AU2092983A (en) * 1982-11-08 1984-05-17 Genentech Inc. Porcine growth hormone produced by recombinant dna technology
EP0111814A3 (en) * 1982-12-16 1985-08-14 Mgi Pharma, Inc. The cloning and expression of nucleotide sequences encoding bovine growth hormone
DE3247922A1 (de) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten
JPS6011557A (ja) * 1983-04-19 1985-01-21 ジエネツクス・コ−ポレイシヨン クロ−ンヒツジ成長ホルモン遺伝子
US4859600A (en) * 1983-04-25 1989-08-22 Genentech, Inc. Recombinant procaryotic cell containing correctly processed human growth hormone
US4755465A (en) * 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US5198361A (en) * 1983-07-15 1993-03-30 Bio-Technology General Corp. Plasmids for production of met-asp-gln bovine growth hormone, hosts containing the plasmids, products manufactured thereby and related methods
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US4521409A (en) * 1983-10-03 1985-06-04 Cornell Research Foundation, Inc. Use of growth hormone to enhance ruminant mammary development
DE3484926D1 (de) * 1983-12-23 1991-09-19 Pfizer Expressionsplasmide fuer die herstellung eines heterologen proteins in bakterien.
US4935370A (en) * 1983-12-23 1990-06-19 Pfizer Inc. Expression plasmids for improved production of heterologous protein in bacteria
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
US5037806A (en) 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
US4861868A (en) 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
ATE76435T1 (de) 1985-06-26 1992-06-15 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US4786501A (en) * 1985-07-15 1988-11-22 International Minerals & Chemical Corp. Cylindrical implants for the controlled release of growth hormones
WO1987001708A2 (en) * 1985-09-18 1987-03-26 The Upjohn Company Enhanced bioactivity of mammalian somatotropin through selective deamidation
US5631227A (en) * 1985-09-18 1997-05-20 The Upjohn Company Somatotropin analogs
ES2019874B3 (es) * 1986-10-08 1991-07-16 Upjohn Co Bioactividad mejorada de somatotropina de mamiferos mediante desamidacion selectiva.
US5013653A (en) * 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
US5268284A (en) * 1988-03-11 1993-12-07 The Upjohn Company Ribosome binding site
JP2787729B2 (ja) * 1990-08-02 1998-08-20 株式会社ニッポンジーン 組換え型ミンク成長ホルモン遺伝子、組換え型ミンクプレ成長ホルモン遺伝子、ミンク成長ホルモン又はミンクプレ成長ホルモン形質転換プラスミド、及び、ミンク成長ホルモン又はミンクプレ成長ホルモン形質転換大腸菌
KR100847386B1 (ko) 2000-12-26 2008-07-18 몬산토 테크놀로지 엘엘씨 소마토트로핀의 발현을 위한 재조합 ｄｎａ 벡터

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0001929B1 (en) * 1977-11-08 1989-04-19 Genentech, Inc. Plasmid for transforming bacterial host to render it capable of polypeptide expression
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
GR68404B (https=) * 1979-06-01 1981-12-29 Univ California
IL59690A (en) * 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Мероу Дж.,Уозит Дк.,Эфетратиадис А. Исследование фибриона шелка.- В кн.: Рекомбинантные молекулы, значение дл науки и практики. Перченод с; ,англ. - M.tj Наука, 1977, К 7, с. 476- 484.. 1 Изобретение относитс к г,еннай инженерии и касаетс способа получени рекомбинантной плазмидной ДНК, кодирующей бычий гормон роста. Рекомбинантна плазмидна ДНК, кодирующа гормон роста быка, получена Путем вьщелеии мРНК , из гипофиз арнык желез быков с последуюпцвч получением кДНК и клонированием полученной кДНК в pBR 3212 Пример 1 Гипофизарии коров извлекают вскоре после умерщвлени коров и сразу же замораживают в жидком азоте. Рибонуклеиновую кислоту (РНК) приготавливают путем гомогенизировани гипофизарйев в растворе тиоцианата гуанидина. Затем РНК экстрагируют фенолом и осаждают в этаноле. Полиаденилированную РНК очищают, использу метод адсорбционной *

Also Published As

Publication number Publication date
CA1225948A (en) 1987-08-25
NZ198050A (en) 1984-12-14
EP0047600A3 (en) 1982-10-20
HU195535B (en) 1988-05-30
YU206281A (en) 1984-08-31
AU7449681A (en) 1982-03-04
CZ636081A3 (en) 1995-02-15
EP0047600B1 (en) 1992-02-26
IL63628A0 (en) 1981-11-30
ES8305828A1 (es) 1983-04-16
PT73569A (en) 1981-09-01
AU544453B2 (en) 1985-05-30
IL63628A (en) 1991-06-10
DK372881A (da) 1982-02-27
JP2763024B2 (ja) 1998-06-11
DD204711A5 (de) 1983-12-07
YU44962B (en) 1991-06-30
JPS57171999A (en) 1982-10-22
DD211581A5 (de) 1984-07-18
PL232797A1 (en) 1983-07-18
JP2534221B2 (ja) 1996-09-11
DD212267A5 (de) 1984-08-08
EP0047600A2 (en) 1982-03-17
JPH09135691A (ja) 1997-05-27
FI812614A7 (fi) 1982-02-27
PH22847A (en) 1989-01-19
PT73569B (en) 1982-11-09
GR74350B (https=) 1984-06-26
DE3177273D1 (de) 1992-04-02
ES514526A0 (es) 1983-04-16
RO105533B1 (ro) 1995-10-01
ES8405072A1 (es) 1984-05-16
CZ280596B6 (cs) 1996-03-13
IE52755B1 (en) 1988-02-17
FI812614L (fi) 1982-02-27
IE811822L (en) 1982-02-26
ES504968A0 (es) 1984-05-16
DD212403A5 (de) 1984-08-15
PH21216A (en) 1987-08-21
ATE72833T1 (de) 1992-03-15
KR830007822A (ko) 1983-11-07
ZA815696B (en) 1982-08-25

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