SU1232148A3 - Способ получени инсулина человека - Google Patents
Способ получени инсулина человека Download PDFInfo
- Publication number
- SU1232148A3 SU1232148A3 SU833582755A SU3582755A SU1232148A3 SU 1232148 A3 SU1232148 A3 SU 1232148A3 SU 833582755 A SU833582755 A SU 833582755A SU 3582755 A SU3582755 A SU 3582755A SU 1232148 A3 SU1232148 A3 SU 1232148A3
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- phe
- insulin
- human insulin
- concentration
- dai
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 8
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title description 18
- 108090001061 Insulin Proteins 0.000 title description 8
- 102000004877 Insulin Human genes 0.000 title description 8
- 229940125396 insulin Drugs 0.000 title description 8
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 claims abstract description 14
- 101000976075 Homo sapiens Insulin Proteins 0.000 claims abstract description 12
- 102000000496 Carboxypeptidases A Human genes 0.000 claims 1
- 108010080937 Carboxypeptidases A Proteins 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 6
- 150000001413 amino acids Chemical class 0.000 abstract description 5
- 150000002148 esters Chemical class 0.000 abstract 3
- 150000001408 amides Chemical class 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
- 239000000203 mixture Substances 0.000 description 8
- 239000011541 reaction mixture Substances 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108010053229 Lysyl endopeptidase Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- 102000005367 Carboxypeptidases Human genes 0.000 description 1
- 108010006303 Carboxypeptidases Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000993800 Sus scrofa Insulin Proteins 0.000 description 1
- VEIKMWOMUYMMMK-FCLVOEFKSA-N Thr-Phe-Phe Chemical compound C([C@H](NC(=O)[C@@H](N)[C@H](O)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 VEIKMWOMUYMMMK-FCLVOEFKSA-N 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- -1 carboxy- Chemical class 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- GKZIWHRNKRBEOH-UHFFFAOYSA-N phenylalanylphenylalanine Chemical group C=1C=CC=CC=1CC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 GKZIWHRNKRBEOH-UHFFFAOYSA-N 0.000 description 1
- 108010073025 phenylalanylphenylalanine Proteins 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/26—Containing cys-cys disulfide bridge between nonadjacent cysteine residues
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL8201650A NL8201650A (nl) | 1982-04-21 | 1982-04-21 | Semisynthetische bereiding van humane insuline. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1232148A3 true SU1232148A3 (ru) | 1986-05-15 |
Family
ID=19839621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU833582755A SU1232148A3 (ru) | 1982-04-21 | 1983-04-20 | Способ получени инсулина человека |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US4840897A (enExample) |
| EP (1) | EP0092280B1 (enExample) |
| JP (2) | JPS58189149A (enExample) |
| AT (1) | ATE42203T1 (enExample) |
| AU (1) | AU554050B2 (enExample) |
| CA (1) | CA1202587A (enExample) |
| DE (1) | DE3379640D1 (enExample) |
| DK (1) | DK173007B1 (enExample) |
| ES (1) | ES8405760A1 (enExample) |
| HU (1) | HU189268B (enExample) |
| IL (1) | IL68397A (enExample) |
| NL (1) | NL8201650A (enExample) |
| PL (1) | PL143571B1 (enExample) |
| SU (1) | SU1232148A3 (enExample) |
| ZA (1) | ZA832602B (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2164520C2 (ru) * | 1993-09-17 | 2001-03-27 | Ново Нордиск А/С | Производное инсулина, растворимая пролонгированная фармацевтическая композиция, способ пролонгирования гипогликемического действия при лечении диабета |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3326472A1 (de) * | 1983-07-22 | 1985-02-14 | Hoechst Ag, 6230 Frankfurt | Neue insulin-derivate, verfahren zu deren herstellung und deren verwendung sowie pharmazeutische mittel zur behandlung des diabetes mellitus |
| DE3326473A1 (de) * | 1983-07-22 | 1985-01-31 | Hoechst Ag, 6230 Frankfurt | Pharmazeutisches mittel zur behandlung des diabetes mellitus |
| DE3327709A1 (de) * | 1983-07-29 | 1985-02-07 | Hoechst Ag, 6230 Frankfurt | Insulin-derivat-kristallsuspensionen, verfahren zu deren herstellung und deren verwendung |
| DE3333640A1 (de) * | 1983-09-17 | 1985-04-25 | Hoechst Ag, 6230 Frankfurt | Verfahren zur herstellung von insulin-derivaten, deren b-kette c-terminal verlaengert ist, neue basisch modifizierte insulin-derivate diese enthaltende mittel und ihre verwendung |
| DK309184D0 (da) * | 1984-06-25 | 1984-06-25 | Nordisk Insulinlab | Fremgangsmaade til isolering af insulin eller insulinlignende materiale fra en fermenteringsvaeske |
| CN113956326B (zh) * | 2021-09-15 | 2024-05-28 | 河南工业大学 | 一种短肽单体，结构自愈型肽基水凝胶及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3276961A (en) * | 1963-04-08 | 1966-10-04 | Squibb & Sons Inc | Process for preparing human insulin |
| US4182654A (en) * | 1974-09-18 | 1980-01-08 | Pierce Chemical Company | Production of polypeptides using polynucleotides |
| NO153061C (no) * | 1979-04-13 | 1986-01-08 | Shionogi & Co | Fremgangsmaate for fremstilling av et b30-threonininsulin |
| JPS55138393A (en) * | 1979-04-13 | 1980-10-29 | Shionogi & Co Ltd | Semisynthesis of insulin |
| JPS55138391A (en) * | 1979-04-13 | 1980-10-29 | Shionogi & Co Ltd | New synthetic method of peptide derivative |
| US4343898A (en) * | 1980-02-11 | 1982-08-10 | Novo Industri A/S | Process for preparing esters of human insulin |
| DK147437A (en) * | 1980-02-11 | 1900-01-01 | Process for preparing human insulin or threonine B30 esters of human insulin, or a salt or complex thereof | |
| DK319780A (da) * | 1980-07-24 | 1982-01-25 | Forenede Bryggerier As | Fremgangsmaade til enzymatisk udskiftning af b-30 aminosyren i insuliner |
| DE3327928A1 (de) * | 1983-08-03 | 1985-02-21 | Hoechst Ag, 6230 Frankfurt | Verfahren zur herstellung von insulin-derivaten |
-
1982
- 1982-04-21 NL NL8201650A patent/NL8201650A/nl not_active Application Discontinuation
-
1983
- 1983-04-13 DK DK198301616A patent/DK173007B1/da not_active IP Right Cessation
- 1983-04-13 ZA ZA832602A patent/ZA832602B/xx unknown
- 1983-04-14 EP EP83200530A patent/EP0092280B1/en not_active Expired
- 1983-04-14 DE DE8383200530T patent/DE3379640D1/de not_active Expired
- 1983-04-14 IL IL68397A patent/IL68397A/xx not_active IP Right Cessation
- 1983-04-14 AT AT83200530T patent/ATE42203T1/de not_active IP Right Cessation
- 1983-04-15 AU AU13554/83A patent/AU554050B2/en not_active Expired
- 1983-04-20 PL PL1983241575A patent/PL143571B1/pl unknown
- 1983-04-20 ES ES521674A patent/ES8405760A1/es not_active Expired
- 1983-04-20 SU SU833582755A patent/SU1232148A3/ru active
- 1983-04-20 JP JP58069862A patent/JPS58189149A/ja active Granted
- 1983-04-20 CA CA000426236A patent/CA1202587A/en not_active Expired
- 1983-04-21 HU HU831392A patent/HU189268B/hu unknown
-
1985
- 1985-08-20 US US06/767,481 patent/US4840897A/en not_active Expired - Lifetime
-
1992
- 1992-10-28 JP JP4290556A patent/JPH0735399B2/ja not_active Expired - Lifetime
Non-Patent Citations (1)
| Title |
|---|
| Schraitt E.W. et al. Норре Seyler s Z.-Physiol. Chemil, 1978, v. 359, p. 799. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2164520C2 (ru) * | 1993-09-17 | 2001-03-27 | Ново Нордиск А/С | Производное инсулина, растворимая пролонгированная фармацевтическая композиция, способ пролонгирования гипогликемического действия при лечении диабета |
Also Published As
| Publication number | Publication date |
|---|---|
| US4840897A (en) | 1989-06-20 |
| JPH05339290A (ja) | 1993-12-21 |
| DK173007B1 (da) | 1999-11-08 |
| NL8201650A (nl) | 1983-11-16 |
| JPS58189149A (ja) | 1983-11-04 |
| AU554050B2 (en) | 1986-08-07 |
| JPH0533993B2 (enExample) | 1993-05-20 |
| ES521674A0 (es) | 1984-06-16 |
| AU1355483A (en) | 1983-10-27 |
| CA1202587A (en) | 1986-04-01 |
| EP0092280B1 (en) | 1989-04-19 |
| HU189268B (en) | 1986-06-30 |
| IL68397A0 (en) | 1983-07-31 |
| DE3379640D1 (en) | 1989-05-24 |
| IL68397A (en) | 1986-01-31 |
| PL241575A1 (en) | 1983-12-19 |
| EP0092280A1 (en) | 1983-10-26 |
| PL143571B1 (en) | 1988-02-29 |
| ZA832602B (en) | 1984-01-25 |
| ATE42203T1 (de) | 1989-05-15 |
| DK161683A (da) | 1983-10-22 |
| DK161683D0 (da) | 1983-04-13 |
| JPH0735399B2 (ja) | 1995-04-19 |
| ES8405760A1 (es) | 1984-06-16 |
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