SG2014014377A - Cell-free preparation of carbapenems - Google Patents

Cell-free preparation of carbapenems

Info

Publication number
SG2014014377A
SG2014014377A SG2014014377A SG2014014377A SG2014014377A SG 2014014377 A SG2014014377 A SG 2014014377A SG 2014014377 A SG2014014377 A SG 2014014377A SG 2014014377 A SG2014014377 A SG 2014014377A SG 2014014377 A SG2014014377 A SG 2014014377A
Authority
SG
Singapore
Prior art keywords
carbapenems
cell
free preparation
preparation
free
Prior art date
Application number
SG2014014377A
Other languages
English (en)
Inventor
William Jeremy Blake
Daniel Klein-Marcuschamer
Original Assignee
Greenlight Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenlight Biosciences Inc filed Critical Greenlight Biosciences Inc
Publication of SG2014014377A publication Critical patent/SG2014014377A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
    • C12P17/184—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system containing a beta-lactam ring, e.g. thienamycin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01041—Glutamate-5-semialdehyde dehydrogenase (1.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/99—Oxidoreductases acting on the CH-NH group of donors (1.5) with other acceptors (1.5.99)
    • C12Y105/99008—Proline dehydrogenase (1.5.99.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02011—Glutamate 5-kinase (2.7.2.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/03—Cyclo-ligases (6.3.3)
    • C12Y603/03004—(Carboxyethyl)arginine beta-lactam-synthase (6.3.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04016—Carbamoyl-phosphate synthase (ammonia) (6.3.4.16)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/05—Carbon-nitrogen ligases with glutamine as amido-N-donor (6.3.5)
    • C12Y603/05005—Carbamoyl-phosphate synthase (glutamine-hydrolysing) (6.3.5.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Enzymes And Modification Thereof (AREA)
SG2014014377A 2011-09-09 2012-09-07 Cell-free preparation of carbapenems SG2014014377A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161533039P 2011-09-09 2011-09-09
PCT/US2012/054195 WO2013036787A2 (en) 2011-09-09 2012-09-07 Cell-free preparation of carbapenems

Publications (1)

Publication Number Publication Date
SG2014014377A true SG2014014377A (en) 2014-05-29

Family

ID=46940589

Family Applications (1)

Application Number Title Priority Date Filing Date
SG2014014377A SG2014014377A (en) 2011-09-09 2012-09-07 Cell-free preparation of carbapenems

Country Status (9)

Country Link
US (2) US9469861B2 (enExample)
EP (1) EP2753702B1 (enExample)
JP (1) JP2014526250A (enExample)
KR (1) KR20150010932A (enExample)
CN (1) CN104093848A (enExample)
CA (1) CA2846893A1 (enExample)
IL (1) IL231010A0 (enExample)
SG (1) SG2014014377A (enExample)
WO (1) WO2013036787A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2463181B (en) 2007-05-14 2013-03-27 Univ New York State Res Found Induction of a physiological dispersion response in bacterial cells in a biofilm
US9637746B2 (en) 2008-12-15 2017-05-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
WO2011140516A2 (en) 2010-05-07 2011-11-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
WO2012030980A1 (en) 2010-08-31 2012-03-08 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
EP2935598A1 (en) 2012-12-21 2015-10-28 Greenlight Biosciences Inc. Cell-free system for converting methane into fuel, pyruvate or isobutanol
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
CA2980172A1 (en) 2015-03-30 2016-10-06 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
RU2018138975A (ru) 2016-04-06 2020-05-12 Гринлайт Байосайенсис, Инк. Бесклеточная продукция рибонуклеиновой кислоты
NL2017636B1 (en) 2016-10-18 2018-04-26 Univ Delft Tech Methods for increasing production of carbapenem antibiotics and derivatives in bacteria
KR20190100386A (ko) 2017-01-06 2019-08-28 그린라이트 바이오사이언시스, 아이엔씨. 당의 무세포 생산
CN107630054A (zh) * 2017-04-13 2018-01-26 绍兴百茵生物技术有限公司 培南类药物中间体的生物转化方法
SG11202003192UA (en) 2017-10-11 2020-05-28 Greenlight Biosciences Inc Methods and compositions for nucleoside triphosphate and ribonucleic acid production
WO2019090205A1 (en) 2017-11-06 2019-05-09 Snap Bio, Inc. Pim kinase inhibitor compositions, methods, and uses thereof
NL2020614B1 (en) * 2018-03-19 2019-09-30 Univ Delft Tech Method for producing cell wall-targeting antibiotics in susceptible bacteria

Family Cites Families (130)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4841555B1 (enExample) 1969-08-04 1973-12-07
USRE28886E (en) 1969-08-04 1976-06-29 Kyowa Hakko Kogyo Co., Ltd. Method for preparing cytidine diphosphate choline
US3950357A (en) 1974-11-25 1976-04-13 Merck & Co., Inc. Antibiotics
US4006060A (en) 1974-11-25 1977-02-01 Merck & Co., Inc. Thienamycin production
US4194047A (en) 1975-11-21 1980-03-18 Merck & Co., Inc. Substituted N-methylene derivatives of thienamycin
US4266034A (en) 1978-04-14 1981-05-05 Exxon Research And Engineering Company Method for producing microbial cells and use thereof to produce oxidation products
US4248966A (en) 1979-05-17 1981-02-03 Massachusetts Institute Of Technology Synthesis of isopenicillin derivatives in the absence of living cells
US4270537A (en) 1979-11-19 1981-06-02 Romaine Richard A Automatic hypodermic syringe
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4329481A (en) 1980-06-25 1982-05-11 Merck & Co., Inc. Process for the preparation of N-protected N-formimidoyl 2-aminoethanethiol
US4292436A (en) 1980-06-25 1981-09-29 Merck & Co., Inc. Process for the preparation of N-protected N-formimidoyl 2-aminoethanethiol
US4374772A (en) 1981-03-19 1983-02-22 Merck & Co., Inc. Process for the preparation of N-formimidoyl thienamycin and reagents therefor
JPS585189A (ja) 1981-07-01 1983-01-12 Sanraku Inc 新規なアミドヒドロラ−ゼ
US4460689A (en) 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
JPS617788A (ja) 1984-06-22 1986-01-14 Sony Corp 静止画記録再生装置
US4596556A (en) 1985-03-25 1986-06-24 Bioject, Inc. Hypodermic injection apparatus
DE3660768D1 (en) 1985-05-13 1988-10-27 Unitika Ltd Process for producing physiologically active substance
JPS61260895A (ja) 1985-05-13 1986-11-19 Unitika Ltd 生理活性物質の製造方法
US5672497A (en) 1986-03-21 1997-09-30 Eli Lilly And Company Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms
US4886499A (en) 1986-12-18 1989-12-12 Hoffmann-La Roche Inc. Portable injection appliance
US4946783A (en) 1987-01-30 1990-08-07 President And Fellows Of Harvard College Periplasmic protease mutants of Escherichia coli
GB8704027D0 (en) 1987-02-20 1987-03-25 Owen Mumford Ltd Syringe needle combination
US4790824A (en) 1987-06-19 1988-12-13 Bioject, Inc. Non-invasive hypodermic injection device
US4940460A (en) 1987-06-19 1990-07-10 Bioject, Inc. Patient-fillable and non-invasive hypodermic injection device assembly
US4941880A (en) 1987-06-19 1990-07-17 Bioject, Inc. Pre-filled ampule and non-invasive hypodermic injection device assembly
US4950603A (en) 1987-11-02 1990-08-21 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii
JPH01228473A (ja) 1988-03-08 1989-09-12 Hikari Kimura 新規な組み換え体ｄｎａおよびグルタチオンの製造法
US5339163A (en) 1988-03-16 1994-08-16 Canon Kabushiki Kaisha Automatic exposure control device using plural image plane detection areas
US5070020A (en) 1988-05-09 1991-12-03 Eli Lilly And Company Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase
FR2638359A1 (fr) 1988-11-03 1990-05-04 Tino Dalto Guide de seringue avec reglage de la profondeur de penetration de l'aiguille dans la peau
IL92740A0 (en) 1988-12-22 1990-09-17 Lilly Co Eli Recombinant dna expression vectors and dna compounds that encode isopenicillin n epimerase(racemase)activity
US5312335A (en) 1989-11-09 1994-05-17 Bioject Inc. Needleless hypodermic injection device
US5064413A (en) 1989-11-09 1991-11-12 Bioject, Inc. Needleless hypodermic injection device
IL97000A0 (en) 1990-01-26 1992-03-29 Lilly Co Eli Recombinant dna expression vectors and dna compounds that encode isopenicillin n epimerase activity
US5190521A (en) 1990-08-22 1993-03-02 Tecnol Medical Products, Inc. Apparatus and method for raising a skin wheal and anesthetizing skin
US5527288A (en) 1990-12-13 1996-06-18 Elan Medical Technologies Limited Intradermal drug delivery device and method for intradermal delivery of drugs
GB9118204D0 (en) 1991-08-23 1991-10-09 Weston Terence E Needle-less injector
SE9102652D0 (sv) 1991-09-13 1991-09-13 Kabi Pharmacia Ab Injection needle arrangement
KR0177841B1 (ko) 1992-01-30 1999-04-01 나까무라 간노스께 시티딘 디인산 콜린의 제조방법
US5328483A (en) 1992-02-27 1994-07-12 Jacoby Richard M Intradermal injection device with medication and needle guard
US5383851A (en) 1992-07-24 1995-01-24 Bioject Inc. Needleless hypodermic injection device
US5569189A (en) 1992-09-28 1996-10-29 Equidyne Systems, Inc. hypodermic jet injector
US5334144A (en) 1992-10-30 1994-08-02 Becton, Dickinson And Company Single use disposable needleless injector
US5319122A (en) 1992-11-12 1994-06-07 Merck & Co., Inc. Process for the preparation of benzylformimidate
US6746859B1 (en) 1993-01-15 2004-06-08 Genetics Institute, Llc Cloning of enterokinase and method of use
AU675506B2 (en) 1993-01-15 1997-02-06 Genetics Institute, Llc Cloning of enterokinase and method of use
US5436131A (en) 1993-04-02 1995-07-25 Merck & Co., Inc. Color screening assay for identifying inhibitor resistant HIV protease mutants
WO1995024176A1 (en) 1994-03-07 1995-09-14 Bioject, Inc. Ampule filling device
US5466220A (en) 1994-03-08 1995-11-14 Bioject, Inc. Drug vial mixing and transfer device
KR0131166B1 (ko) 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법
GB9410142D0 (en) 1994-05-20 1994-07-06 Univ Warwick Carbapenems
US5599302A (en) 1995-01-09 1997-02-04 Medi-Ject Corporation Medical injection system and method, gas spring thereof and launching device using gas spring
JPH08196284A (ja) 1995-01-19 1996-08-06 Canon Inc 酵素反応素子およびその製造方法、酵素反応器ならびに酵素反応方法
US5730723A (en) 1995-10-10 1998-03-24 Visionary Medical Products Corporation, Inc. Gas pressured needle-less injection device and method
EP1493814A1 (en) 1995-07-06 2005-01-05 The Leland Stanford Junior University Cell-free synthesis of polyketides
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5893397A (en) 1996-01-12 1999-04-13 Bioject Inc. Medication vial/syringe liquid-transfer apparatus
GB9607549D0 (en) 1996-04-11 1996-06-12 Weston Medical Ltd Spring-powered dispensing device
KR100205768B1 (en) 1996-08-24 1999-07-01 Choongwae Pharm Co Stereo-selective composition of 4-acetoxyazetidinone
GB9622516D0 (en) 1996-10-29 1997-01-08 Univ Cambridge Tech Enzymic cofactor cycling
WO1998030713A1 (en) 1997-01-14 1998-07-16 Bio-Technical Resources Process for production of n-glucosamine
US5993412A (en) 1997-05-19 1999-11-30 Bioject, Inc. Injection apparatus
IT1298087B1 (it) 1998-01-08 1999-12-20 Fiderm S R L Dispositivo per il controllo della profondita' di penetrazione di un ago, in particolare applicabile ad una siringa per iniezioni
GB9815666D0 (en) * 1998-07-17 1998-09-16 Smithkline Beecham Plc Novel compounds
US6613552B1 (en) 1999-01-29 2003-09-02 Board Of Trustees Operating Michigan State University Biocatalytic synthesis of shikimic acid
US6168931B1 (en) 1999-03-17 2001-01-02 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of biological macromolecules using a novel ATP regeneration system
JP4707237B2 (ja) 1999-03-17 2011-06-22 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 外因性アミノ酸および新規ａｔｐ再生システムを用いたインビトロ高分子生合成の方法
US6994986B2 (en) 1999-03-17 2006-02-07 The Board Of Trustees Of The Leland Stanford University In vitro synthesis of polypeptides by optimizing amino acid metabolism
JP2003506011A (ja) 1999-04-15 2003-02-18 ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニア ソーターゼ遺伝子の同定
US6284483B1 (en) 1999-10-06 2001-09-04 Board Of Trustees Operating Michigan State University Modified synthetases to produce penicillins and cephalosporins under the control of bicarbonate
CA2293852A1 (fr) 1999-12-30 2001-06-30 Purecell Technologies Inc. Procede de preparation d'extraits de plantes actifs et utiles a la capture de radicaux libres; les extraits, et compositions et dispositifs les comprenant
US6548276B2 (en) 2000-09-06 2003-04-15 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
US7041479B2 (en) 2000-09-06 2006-05-09 The Board Of Trustess Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
KR100932882B1 (ko) 2001-04-04 2009-12-21 제넨코 인터내셔날 인코포레이티드 숙주 세포에서의 생산물의 생산 방법
US7112434B2 (en) 2001-05-02 2006-09-26 University Of South Florida Vector system for selection of genes encoding secreted proteins and membrane-bound proteins
JPWO2003027301A1 (ja) 2001-09-06 2005-01-06 味の素株式会社 微生物を用いたアルコールの製造法
ES2329880T3 (es) 2001-09-13 2009-12-02 Genentech, Inc. Aminopeptidasa b2324.
US20030113778A1 (en) 2001-10-30 2003-06-19 Schulte Jennifer Theresa Enhanced in vitro nucleic acid synthesis using nucleoside monophosphates
US20040038250A1 (en) 2002-04-04 2004-02-26 Astur-Pharma, S.A. Gene cluster for thienamycin biosynthesis, genetic manipulation and utility
DE10219714A1 (de) 2002-05-02 2003-11-27 Holland Sweetener Co Verfahren zur mikrobielien Herstellung von aromatischen Aminosäuren und anderen Metaboliten des aromatischen Aminosäurebiosyntheseweges
EP1502956B1 (en) 2002-05-08 2009-07-29 Kyowa Hakko Bio Co., Ltd Process for producing cytidine 5'-diphosphate choline
US7332304B2 (en) 2002-07-01 2008-02-19 Arkion Life Sciences Llc Process and materials for production of glucosamine and N-acetylglucosamine
AU2003259912B2 (en) 2002-08-19 2008-10-23 The Board Of Trustees Of The Leland Stanford Junior University Improved methods of in vitro protein synthesis
JP2007502623A (ja) 2003-05-09 2007-02-15 リサーチ ディベロップメント ファウンデーション 膜タンパク質におけるフリンプロテアーゼ開裂部位の挿入およびその使用法
US20050054044A1 (en) 2003-07-18 2005-03-10 The Board Of Trustees Of The Leland Stanford Junior University Method of alleviating nucleotide limitations for in vitro protein synthesis
US7341852B2 (en) 2003-07-18 2008-03-11 The Board Of Trustees Of The Leland Stanford Junior University Methods of decoupling reaction scale and protein synthesis yield in batch mode
WO2005030949A1 (en) 2003-09-24 2005-04-07 Board Of Trustees Operating Michigan State University Methods and materials for the production of shikimic acid
ES2532608T3 (es) 2003-11-20 2015-03-30 The Board Of Trustees Of The Leland Stanford Junior University Métodos mejorados de síntesis proteica in vitro
WO2005098048A1 (en) 2004-03-25 2005-10-20 The Board Of Trustees Of The Leland Stanford Junior University Protein expression yield enhancement in cell-free protein synthesis systems by addition of antifoam agents
CN1973037B (zh) 2004-06-25 2011-10-19 协和发酵生化株式会社 二肽或二肽衍生物的制造方法
CN101098963A (zh) 2004-12-10 2008-01-02 帝斯曼知识产权资产管理有限公司 通过经遗传修饰的非天然生产微生物来生产β-内酰胺
WO2006090385A2 (en) 2005-02-22 2006-08-31 Ramot At Tel-Aviv University Ltd. Protease inhibitors and method of screening thereof
US7351563B2 (en) 2005-06-10 2008-04-01 The Board Of Trustees Of The Leland Stanford Junior University Cell-free extracts and synthesis of active hydrogenase
US7312049B2 (en) 2005-06-14 2007-12-25 The Board Of Trustees Of The Leland Stanford Junior University Total amino acid stabilization during cell-free protein synthesis
TW200741005A (en) 2005-08-10 2007-11-01 Kyowa Hakko Kogyo Kk A purification method of cytidine diphosphate
WO2007030772A2 (en) 2005-09-09 2007-03-15 The Johns Hopkins University Improved production of clavulanic acid by genetic engineering of streptomyces clavuligerus
US8183010B2 (en) 2005-10-31 2012-05-22 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of membrane bound polypeptides
WO2007137144A2 (en) 2006-05-17 2007-11-29 University Of Medicine And Dentistry Of New Jersey Single protein production in living cells facilitated by a messenger rna interferase
WO2007140816A1 (en) 2006-06-09 2007-12-13 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
WO2007144018A1 (en) 2006-06-12 2007-12-21 Metabolic Explorer Ethanolamine production by fermentation
WO2008002663A2 (en) 2006-06-28 2008-01-03 The Board Of Trustees Of The Leland Stanford Junior University Immunogenic protein constructs
WO2008002661A2 (en) 2006-06-28 2008-01-03 The Board Of Trustees Of The Leland Stanford Junior University Fusion protein constructs
EP2035554B1 (en) 2006-06-29 2013-04-24 The Board of Trustees of The Leland Stanford Junior University Cell-free synthesis of proteins containing unnatural amino acids
WO2008002673A2 (en) 2006-06-29 2008-01-03 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of virus like particles
WO2008062279A2 (en) 2006-11-20 2008-05-29 Orchid Chemicals & Pharmaceuticals Limited An improved process for the preparation of carbapenem antibiotic
KR100832740B1 (ko) 2007-01-17 2008-05-27 한국과학기술원 분지쇄 아미노산 생성능이 개선된 변이 미생물 및 이를이용한 분지쇄 아미노산의 제조방법
WO2008088884A2 (en) 2007-01-18 2008-07-24 The Board Of Trustees Of The Leland Stanford Junior University Enhanced cell-free synthesis of active proteins containing disulfide bonds
WO2008094546A2 (en) 2007-01-31 2008-08-07 The Regents Of The University Of California Genetically modified host cells for increased p450 activity levels and methods of use thereof
US20090124012A1 (en) 2007-08-08 2009-05-14 Mazef Biosciences, Llc Toxin/antitoxin systems and methods for regulating cellular growth, metabolic engineering and production of recombinant proteins
WO2009102205A1 (en) 2008-02-14 2009-08-20 Wageningen Universiteit Nucleotide sequences coding for cis-aconitic decarboxylase and use thereof
JP5755884B2 (ja) 2008-03-05 2015-07-29 ジェノマティカ， インコーポレイテッド 第一級アルコールを産生する生物
JP2011519561A (ja) 2008-05-01 2011-07-14 ジェノマティカ， インコーポレイテッド メタクリル酸の産生のための微生物
DK2313489T3 (da) 2008-07-28 2015-06-22 Brain Biotechnology Res & Information Network Ag Fremstillingsmetode
GB0819563D0 (en) 2008-10-24 2008-12-03 Isis Innovation Methods for preparing heterocyclic rings
BRPI0914521A2 (pt) 2008-10-27 2016-07-26 Butamax Advanced Biofuels Llc célula hospedeira microbiana recombinante, método de aumento da produção de isobutanol e método de produção de isobutanol
US20100143997A1 (en) 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions
US9637746B2 (en) 2008-12-15 2017-05-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
EP2379728A4 (en) * 2008-12-22 2016-04-13 Greenlight Biosciences Inc COMPOSITIONS AND METHODS FOR THE PRODUCTION OF A COMPOUND
DK2204453T3 (da) 2008-12-30 2013-06-10 Sued Chemie Ip Gmbh & Co Kg Fremgangsmåde til celle-fri fremstilling af kemikalier
US8597923B2 (en) 2009-05-06 2013-12-03 SyntheZyme, LLC Oxidation of compounds using genetically modified Candida
US10385367B2 (en) 2009-06-01 2019-08-20 Ginkgo Bioworks, Inc. Methods and molecules for yield improvement involving metabolic engineering
EP2462221B1 (en) 2009-08-05 2017-02-22 Genomatica, Inc. Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
CA2783962C (en) 2009-12-11 2016-07-26 The Johns Hopkins University Method for late introduction of the (8r)-hydroxyl group in carbapenem .beta.-lactam antibiotic synthesis
WO2011130544A2 (en) 2010-04-14 2011-10-20 Sutro Biopharma, Inc. Monitoring a dynamic system by liquid chromatography-mass spectrometry
WO2011140516A2 (en) 2010-05-07 2011-11-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
WO2012030980A1 (en) 2010-08-31 2012-03-08 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
WO2012135902A1 (en) 2011-04-08 2012-10-11 James Cook University Protease activity assay
EP2935598A1 (en) 2012-12-21 2015-10-28 Greenlight Biosciences Inc. Cell-free system for converting methane into fuel, pyruvate or isobutanol
CN105378076A (zh) 2013-06-05 2016-03-02 绿光生物科技股份有限公司 在无细胞生物合成系统中对代谢通量的控制
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site

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US9469861B2 (en) 2016-10-18
CN104093848A (zh) 2014-10-08
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