SG187699A1 - Simplified basic media for human pluripotent cell culture - Google Patents

Simplified basic media for human pluripotent cell culture Download PDF

Info

Publication number
SG187699A1
SG187699A1 SG2013008347A SG2013008347A SG187699A1 SG 187699 A1 SG187699 A1 SG 187699A1 SG 2013008347 A SG2013008347 A SG 2013008347A SG 2013008347 A SG2013008347 A SG 2013008347A SG 187699 A1 SG187699 A1 SG 187699A1
Authority
SG
Singapore
Prior art keywords
cells
cell
pluripotent stem
medium
tgf
Prior art date
Application number
SG2013008347A
Other languages
English (en)
Inventor
James A Thomson
Guokai Chen
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of SG187699A1 publication Critical patent/SG187699A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG2013008347A 2010-08-05 2011-08-05 Simplified basic media for human pluripotent cell culture SG187699A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37112810P 2010-08-05 2010-08-05
PCT/US2011/046796 WO2012019122A2 (en) 2010-08-05 2011-08-05 Simplified basic media for human pluripotent cell culture

Publications (1)

Publication Number Publication Date
SG187699A1 true SG187699A1 (en) 2013-03-28

Family

ID=44543813

Family Applications (1)

Application Number Title Priority Date Filing Date
SG2013008347A SG187699A1 (en) 2010-08-05 2011-08-05 Simplified basic media for human pluripotent cell culture

Country Status (12)

Country Link
US (1) US9279103B2 (cg-RX-API-DMAC10.html)
EP (1) EP2601288B1 (cg-RX-API-DMAC10.html)
JP (2) JP6043999B2 (cg-RX-API-DMAC10.html)
KR (1) KR101829488B1 (cg-RX-API-DMAC10.html)
CN (2) CN108531444A (cg-RX-API-DMAC10.html)
AU (1) AU2011285531B2 (cg-RX-API-DMAC10.html)
BR (2) BR112013002811A8 (cg-RX-API-DMAC10.html)
CA (1) CA2807418C (cg-RX-API-DMAC10.html)
DK (1) DK2601288T3 (cg-RX-API-DMAC10.html)
IL (1) IL224431A (cg-RX-API-DMAC10.html)
SG (1) SG187699A1 (cg-RX-API-DMAC10.html)
WO (1) WO2012019122A2 (cg-RX-API-DMAC10.html)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
RU2473685C2 (ru) 2007-07-31 2013-01-27 Лайфскен, Инк. Дифференцировка человеческих эмбриональных стволовых клеток
CN107574142B (zh) 2007-11-27 2021-07-06 生命扫描有限公司 人胚胎干细胞的分化
BRPI0908033A2 (pt) 2008-02-21 2015-08-04 Centocor Ortho Biotech Inc Método placas de superfície modificada e composições para adesão, cultura e desprendimento de célula
CA2729121C (en) 2008-06-30 2019-04-09 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
EP2346988B1 (en) 2008-10-31 2017-05-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
JP2012507289A (ja) 2008-10-31 2012-03-29 ヤンセン バイオテツク,インコーポレーテツド ヒト胚性幹細胞の膵内分泌系への分化
MX2011005288A (es) 2008-11-20 2011-06-01 Centocor Ortho Biotech Inc Celulas madre pluripotentes en microportadores.
CA2744227C (en) 2008-11-20 2018-10-02 Centocor Ortho Biotech Inc. Methods and compositions for cell attachment and cultivation on planar substrates
EP2456862A4 (en) 2009-07-20 2013-02-27 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
DK2516625T3 (da) 2009-12-23 2024-09-09 Janssen Biotech Inc Differentiering af humane embryonale stamceller
AU2011223900A1 (en) 2010-03-01 2012-09-13 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
AU2011250912A1 (en) 2010-05-12 2012-11-22 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
WO2012030540A2 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
CN103221536B (zh) 2010-08-31 2016-08-31 詹森生物科技公司 人胚胎干细胞的分化
KR101836850B1 (ko) 2010-08-31 2018-03-09 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
EP3260140B1 (en) 2011-12-05 2021-02-03 Factor Bioscience Inc. Methods and products for transfecting cells
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
WO2013090919A1 (en) 2011-12-16 2013-06-20 Wisconsin Alumni Research Foundation Fgf-2 having enhanced stability
RU2705001C2 (ru) 2011-12-22 2019-11-01 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в одногормональные инсулинположительные клетки
MX354775B (es) 2012-03-07 2018-03-20 Janssen Biotech Inc Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes.
CN104334719B (zh) 2012-06-08 2018-02-13 詹森生物科技公司 人胚胎干细胞向胰腺内分泌细胞的分化
CA2890110C (en) 2012-11-01 2023-05-02 Factor Bioscience Inc. Methods and products for expressing proteins in cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
KR102084561B1 (ko) 2012-12-31 2020-03-04 얀센 바이오테크 인코포레이티드 췌장 내분비 세포로의 분화를 위한 공기-액체 계면에서의 인간 배아 줄기세포의 배양
DK2938722T3 (da) 2012-12-31 2022-02-14 Janssen Biotech Inc Suspension og klyngedannelse af humane pluripotente celler til differentiering til endokrine pankreasceller
MX2015008578A (es) 2012-12-31 2015-09-07 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas en celulas endocrinas pancreaticas mediante el uso de relugadores de hb9.
EP2952579B1 (en) 2013-01-31 2021-09-01 Ajinomoto Co., Inc. Culture method for stable undifferentiated proliferation of pluripotent stem cells
WO2014208295A1 (ja) 2013-06-28 2014-12-31 株式会社カネカ 多能性幹細胞の増殖促進因子のスクリーニング法
US10137154B2 (en) * 2013-07-12 2018-11-27 Bioventures, Llc Methods and compositions for expanding long-term hematopoietic stem cell populations
KR101655383B1 (ko) * 2013-07-27 2016-09-08 고려대학교 산학협력단 소분자 화합물을 포함하는 만능성 줄기세포의 염색체 안정성 유지용 조성물
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
KR102415811B1 (ko) 2014-01-31 2022-07-04 팩터 바이오사이언스 인크. 핵산 제조 및 송달을 위한 방법 및 산물
JP6422221B2 (ja) * 2014-03-04 2018-11-14 旭化成株式会社 多能性幹細胞からなる細胞塊製造方法
JP6502323B2 (ja) 2014-03-26 2019-04-17 国立大学法人京都大学 多能性幹細胞培養用培地
SG11201609473XA (en) 2014-05-16 2016-12-29 Janssen Biotech Inc Use of small molecules to enhance mafa expression in pancreatic endocrine cells
US20180042969A1 (en) * 2014-08-07 2018-02-15 Duke University Compositions and methods for the reprogramming of cells into cardiomyocytes
US10444227B2 (en) 2014-08-19 2019-10-15 FUJIFILM Cellular Dynamics, Inc. Neural networks formed from cells derived from pluripotent stem cells
US10626372B1 (en) 2015-01-26 2020-04-21 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
US11241505B2 (en) 2015-02-13 2022-02-08 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
US12642852B1 (en) 2015-11-04 2026-06-02 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
CN122146606A (zh) 2015-11-04 2026-06-05 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
WO2017180361A1 (en) 2016-04-14 2017-10-19 Janssen Biotech, Inc. Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
IL264439B2 (en) 2016-08-17 2024-08-01 Factor Bioscience Inc non-viral, cell-free composition comprising a synthetic messenger RNA (MRNA) encoding a gene-editing protein for use in treating cancer, and a synthetic RNA encoding a gene-editing protein for use in treatment
KR101953573B1 (ko) * 2017-03-31 2019-03-05 한양대학교 에리카산학협력단 난황줄기세포 배양을 위한 우태아혈청 조성물 및 이의 제조방법
CN110891967B (zh) * 2017-04-18 2025-03-14 富士胶片细胞动力公司 抗原特异性免疫效应细胞
WO2019049985A1 (ja) 2017-09-08 2019-03-14 日産化学株式会社 細胞保存材料
KR102121602B1 (ko) * 2018-06-27 2020-06-10 주식회사 티아라줄기세포연구소 지방줄기세포배양용 배지 조성물 제조방법, 지방줄기세포배양용 배지 조성물 제조방법과 줄기세포 유효성분 3저 추출법을 이용한 줄기세포파쇄추출물(쉘드줄기세포) 제조방법, 이를 이용한 항관절염 치료용 조성물, 이를 이용한 염증억제 효과를 갖는 조성물 및 세포재생 효과를 갖는 조성물
WO2020200071A1 (en) * 2019-04-05 2020-10-08 The University Of Hong Kong Culture medium for mammalian expanded potential stem cells, composition, and methods thereof
CA3145425A1 (en) 2019-07-03 2021-01-07 Factor Bioscience Inc. Cationic lipids and uses thereof
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
CN115103903A (zh) * 2019-09-19 2022-09-23 西北大学 用于人诱导型多能干细胞的具成本效益的培养基和操作方案
CN110564673A (zh) * 2019-09-26 2019-12-13 广东工业大学 一种干细胞培养液及其应用
CN110564678B (zh) * 2019-10-12 2023-04-07 广东唯泰生物科技有限公司 脐带华通氏胶间充质干细胞成骨定向分化的方法
GB202003618D0 (en) 2020-03-12 2020-04-29 Univ Bristol Gene Therapy
EP4342977A1 (en) * 2022-09-26 2024-03-27 Ares Trading S.A. Serum free medium
WO2024091801A2 (en) * 2022-10-13 2024-05-02 President And Fellows Of Harvard College Methods and compositions for inducing cell differentiation
WO2024218715A1 (en) 2023-04-19 2024-10-24 Btl Healthcare Technologies A.S. Culture media based on protein hydrolysate and a process for preparing thereof
US12668774B2 (en) 2023-10-12 2026-06-30 Btl Healthcare Technologies A.S. Product, system and method of cell cultivation
CN119020297A (zh) * 2024-10-22 2024-11-26 江苏凯基生物技术股份有限公司 一种用于人诱导多能干细胞的化学成分限定培养基
EP4745225A1 (en) * 2024-11-13 2026-05-20 The Cultivated B. GmbH SERUM-FREE NUTRITIVE COMPOSITION FOR VERTEBRA CELL CULTURE

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
BRPI0514778B1 (pt) 2004-09-08 2018-07-10 Wisconsin Alumni Research Foundation Meio para cultivar células tronco embrionárias
WO2007026353A2 (en) * 2005-08-29 2007-03-08 Technion Research & Development Foundation Ltd. Media for culturing stem cells
GB0606671D0 (en) * 2006-04-03 2006-05-10 Plasso Technology Ltd Cell Culture
EP2291511B1 (en) * 2008-05-02 2017-06-21 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells
EP3450545B1 (en) 2008-10-24 2023-08-23 Wisconsin Alumni Research Foundation Pluripotent stem cells obtained by non-viral reprogramming
WO2011058558A2 (en) 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

Also Published As

Publication number Publication date
WO2012019122A2 (en) 2012-02-09
WO2012019122A3 (en) 2012-05-03
JP6043999B2 (ja) 2016-12-14
KR20130099927A (ko) 2013-09-06
EP2601288A2 (en) 2013-06-12
IL224431A (en) 2017-10-31
CN108531444A (zh) 2018-09-14
DK2601288T3 (en) 2016-05-30
CN103180434A (zh) 2013-06-26
CA2807418A1 (en) 2012-02-09
BR112013002811A2 (pt) 2016-08-02
US9279103B2 (en) 2016-03-08
AU2011285531A1 (en) 2013-02-21
JP2013532492A (ja) 2013-08-19
JP2017018137A (ja) 2017-01-26
KR101829488B1 (ko) 2018-02-14
AU2011285531B2 (en) 2015-04-30
EP2601288B1 (en) 2016-04-06
US20120202291A1 (en) 2012-08-09
CA2807418C (en) 2017-04-04
BR122020000909B1 (pt) 2021-02-23
BR112013002811A8 (pt) 2020-01-28

Similar Documents

Publication Publication Date Title
AU2011285531B2 (en) Simplified basic media for human pluripotent cell culture
US9279107B2 (en) Simplified basic media for human pluripotent cell culture
US20230151326A1 (en) Simplified Compositions and Methods for Generating Neural Stem Cells from Human Pluripotent Stem Cells
US10047341B2 (en) Generation of keratinocytes from pluripotent stem cells and maintenance of keratinocyte cultures
US8597947B2 (en) Undifferentiated stem cell culture systems
MX2007002389A (es) Cultivo de celulas progenitoras embrionarias humanas.
WO2013054112A1 (en) Culture media for pluripotent stem cells
US20210189330A1 (en) Induced totipotent stem cells and methods for making and using the same
US11001810B1 (en) Serum-free human pluripotent stem cell culture medium
JP2019519204A (ja) 多能性幹細胞のための培養培地
US7700352B2 (en) Composition for culturing mouse pluripotent stem cells
US8759098B2 (en) Method for cloning pluripotent stem cells
KR102581040B1 (ko) 돼지 만능성 줄기세포 배양용 배지 조성물
US20250051724A1 (en) Medium composition for culturing porcine pluripotent stem cells
US20250059517A1 (en) Media and methods for making and maintaining porcine pluripotent stem cells
HK1261077A1 (en) Simplified basic media for human pluripotent cell culture
Malolina et al. Creation of a model of co-culturing of Sertoli-like mouse cells with spermatogonial cells
WO2025262235A1 (en) Ungulate pluripotent stem cell culture medium
CN120464577A (zh) 用于获得猪多能干细胞的培养基以及猪多能干细胞的诱导培养方法
CN119698464A (zh) 从灵长类的胚胎卵巢细胞诱导卵泡的体外培养法
Young et al. Feeder-free Culture
OJALA Establishing and optimizing feeder cell-free culture methods for human