SG173569A1 - Oligonucleotide probes and primers for detection of hepatitis b virus - Google Patents
Oligonucleotide probes and primers for detection of hepatitis b virus Download PDFInfo
- Publication number
- SG173569A1 SG173569A1 SG2011056645A SG2011056645A SG173569A1 SG 173569 A1 SG173569 A1 SG 173569A1 SG 2011056645 A SG2011056645 A SG 2011056645A SG 2011056645 A SG2011056645 A SG 2011056645A SG 173569 A1 SG173569 A1 SG 173569A1
- Authority
- SG
- Singapore
- Prior art keywords
- seq
- primers
- probes
- hepatitis
- virus
- Prior art date
Links
- 241000700721 Hepatitis B virus Species 0.000 title claims abstract description 66
- 238000001514 detection method Methods 0.000 title claims abstract description 26
- 108020005187 Oligonucleotide Probes Proteins 0.000 title claims abstract description 18
- 239000002751 oligonucleotide probe Substances 0.000 title claims abstract description 18
- 239000003155 DNA primer Substances 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 27
- 239000011541 reaction mixture Substances 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 230000000692 anti-sense effect Effects 0.000 claims abstract description 8
- 239000000523 sample Substances 0.000 claims description 65
- 230000003321 amplification Effects 0.000 claims description 21
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 21
- 238000003753 real-time PCR Methods 0.000 claims description 16
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims description 14
- 230000009977 dual effect Effects 0.000 claims description 13
- 102000039446 nucleic acids Human genes 0.000 claims description 13
- 108020004707 nucleic acids Proteins 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 10
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 8
- 108010006785 Taq Polymerase Proteins 0.000 claims description 7
- 239000000975 dye Substances 0.000 claims description 7
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 7
- 101150003160 X gene Proteins 0.000 claims description 6
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 6
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- 210000002381 plasma Anatomy 0.000 claims description 4
- RGNHAWFQWRAADF-UHFFFAOYSA-N 1-[4-[[4-(dimethylamino)phenyl]diazenyl]phenyl]pyrrole-2,5-dione Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(N2C(C=CC2=O)=O)C=C1 RGNHAWFQWRAADF-UHFFFAOYSA-N 0.000 claims description 3
- 239000005711 Benzoic acid Substances 0.000 claims description 3
- 235000010233 benzoic acid Nutrition 0.000 claims description 3
- 229960000956 coumarin Drugs 0.000 claims description 3
- 235000001671 coumarin Nutrition 0.000 claims description 3
- 150000004775 coumarins Chemical class 0.000 claims description 3
- DLBFLQKQABVKGT-UHFFFAOYSA-L lucifer yellow dye Chemical compound [Li+].[Li+].[O-]S(=O)(=O)C1=CC(C(N(C(=O)NN)C2=O)=O)=C3C2=CC(S([O-])(=O)=O)=CC3=C1N DLBFLQKQABVKGT-UHFFFAOYSA-L 0.000 claims description 3
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 claims description 3
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims description 3
- SJQRQOKXQKVJGJ-UHFFFAOYSA-N 5-(2-aminoethylamino)naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(NCCN)=CC=CC2=C1S(O)(=O)=O SJQRQOKXQKVJGJ-UHFFFAOYSA-N 0.000 claims description 2
- WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 claims 5
- 238000011002 quantification Methods 0.000 abstract description 2
- 239000013615 primer Substances 0.000 description 29
- 108091034117 Oligonucleotide Proteins 0.000 description 16
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 11
- 102000053602 DNA Human genes 0.000 description 11
- 108091093088 Amplicon Proteins 0.000 description 10
- 230000003612 virological effect Effects 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 4
- 239000002987 primer (paints) Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 208000006454 hepatitis Diseases 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000012181 QIAquick gel extraction kit Methods 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-N sulfonic acid Chemical compound OS(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN314CH2009 | 2009-02-13 | ||
| PCT/IN2010/000048 WO2010092595A2 (en) | 2009-02-13 | 2010-01-27 | Oligonucleotide probes and primers for detection of hepatitis b virus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG173569A1 true SG173569A1 (en) | 2011-09-29 |
Family
ID=42562138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2011056645A SG173569A1 (en) | 2009-02-13 | 2010-01-27 | Oligonucleotide probes and primers for detection of hepatitis b virus |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US20110306037A1 (ar) |
| EP (1) | EP2396428A4 (ar) |
| JP (1) | JP2012517804A (ar) |
| KR (1) | KR20110117224A (ar) |
| CN (1) | CN102317474A (ar) |
| AP (1) | AP2011005866A0 (ar) |
| AU (1) | AU2010213426A1 (ar) |
| CA (1) | CA2751745A1 (ar) |
| CL (1) | CL2011001953A1 (ar) |
| CO (1) | CO6420362A2 (ar) |
| EA (1) | EA201171052A1 (ar) |
| IL (1) | IL214514A0 (ar) |
| MA (1) | MA33040B1 (ar) |
| MX (1) | MX2011008587A (ar) |
| NZ (1) | NZ594574A (ar) |
| PE (1) | PE20120856A1 (ar) |
| SG (1) | SG173569A1 (ar) |
| TN (1) | TN2011000401A1 (ar) |
| UA (1) | UA99793C2 (ar) |
| WO (1) | WO2010092595A2 (ar) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101678553B1 (ko) * | 2012-04-30 | 2016-11-22 | (주)바이오니아 | 엡스타인-바 바이러스 검출용 키트 및 이를 이용한 엡스타인-바 바이러스의 검출방법 |
| TWI586809B (zh) * | 2014-11-10 | 2017-06-11 | Taichung Veterans General Hospital | In vitro detection and / or quantification of hepatitis B virus and its use of the primer, probe |
| CN105861649A (zh) * | 2016-04-06 | 2016-08-17 | 苏州华益美生物科技有限公司 | 一种新型核酸扩增反应中的探针及应用 |
| EP3541959A1 (en) | 2016-11-21 | 2019-09-25 | Gen-Probe Incorporated | Compositions and methods for detecting or quantifying hepatitis b virus |
| EP3628058B1 (en) * | 2017-09-27 | 2022-05-11 | Abbott Molecular Inc. | Assay for detecting hepatitis b virus (hbv) |
| CN111808985A (zh) * | 2019-04-11 | 2020-10-23 | 北京大学 | 超敏检测NAs治疗下乙型肝炎病毒DNA的寡核苷酸组合物、试剂盒、方法及用途 |
| CN111154920A (zh) * | 2020-03-09 | 2020-05-15 | 北京康美天鸿生物科技有限公司 | 一种用于检测乙型肝炎病毒的试剂盒及其专用引物探针组 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11262399A (ja) * | 1998-03-17 | 1999-09-28 | Srl Inc | Ｂ型肝炎ウイルス検出用プライマー及びそれを用いたｂ型肝炎ウイルスの検出方法 |
| JP4399048B2 (ja) * | 1999-01-13 | 2010-01-13 | 財団法人 東京都医学研究機構 | リアルタイム検出ｐｃｒ法によるｈｂｖ遺伝子の測定方法並びにそれに用いられるプライマー及びプローブ |
| EP1451352A4 (en) * | 2001-10-09 | 2006-06-21 | Chiron Corp | IDENTIFICATION OF OLIGONUCLEOTIDES FOR DETECTION, DETECTION AND QUANTIFICATION OF HEPATITIS B VIRUS DNA |
| US7015317B2 (en) * | 2002-05-02 | 2006-03-21 | Abbott Laboratories | Polynucleotides for the detection and quantification of hepatitis B virus nucleic acids |
| US20040058314A1 (en) * | 2002-05-29 | 2004-03-25 | Ming Liang He | Assay for the detection and quantification of HBV cccDNA by real-time PCR |
| CN1392268A (zh) * | 2002-06-11 | 2003-01-22 | 赵伟 | 一种检测多种肝炎的检测型基因芯片 |
| KR100647277B1 (ko) * | 2003-08-14 | 2006-11-17 | 삼성전자주식회사 | Ｂ형 간염 검사용 ｐｃｒ 프라이머 세트 및 이를 포함하는 ｂ형 간염 검사 키트 |
| JP4708045B2 (ja) * | 2005-02-10 | 2011-06-22 | 株式会社エスアールエル | Ｂ型肝炎ウイルスｃｃｃＤＮＡの測定方法並びにそのためのプライマー及びプローブ |
| GB0519169D0 (en) * | 2005-09-21 | 2005-10-26 | Leuven K U Res & Dev | Novel anti-viral strategy |
| CN100469895C (zh) * | 2006-06-12 | 2009-03-18 | 山东省医药生物技术研究中心 | 用于乙肝病毒的荧光定量pcr检测试剂盒 |
| JP2009213465A (ja) * | 2007-10-30 | 2009-09-24 | Toshiba Corp | Ｂ型肝炎ウイルス薬剤耐性株検出用核酸プライマーセット、アッセイキットおよびｂ型肝炎ウィルスの薬剤耐性株の検出方法 |
| WO2009122422A1 (en) * | 2008-03-31 | 2009-10-08 | Bigtec Private Limited | Probes and primers for detection of hepatitis b virus and a method thereof |
-
2010
- 2010-01-27 AP AP2011005866A patent/AP2011005866A0/xx unknown
- 2010-01-27 JP JP2011549734A patent/JP2012517804A/ja active Pending
- 2010-01-27 AU AU2010213426A patent/AU2010213426A1/en not_active Abandoned
- 2010-01-27 WO PCT/IN2010/000048 patent/WO2010092595A2/en active Application Filing
- 2010-01-27 CN CN2010800076392A patent/CN102317474A/zh active Pending
- 2010-01-27 NZ NZ594574A patent/NZ594574A/en not_active IP Right Cessation
- 2010-01-27 EP EP10741015A patent/EP2396428A4/en not_active Withdrawn
- 2010-01-27 MX MX2011008587A patent/MX2011008587A/es unknown
- 2010-01-27 UA UAA201110011A patent/UA99793C2/ru unknown
- 2010-01-27 US US13/201,215 patent/US20110306037A1/en not_active Abandoned
- 2010-01-27 EA EA201171052A patent/EA201171052A1/ru unknown
- 2010-01-27 CA CA2751745A patent/CA2751745A1/en not_active Abandoned
- 2010-01-27 MA MA34083A patent/MA33040B1/fr unknown
- 2010-01-27 PE PE2011001490A patent/PE20120856A1/es not_active Application Discontinuation
- 2010-01-27 SG SG2011056645A patent/SG173569A1/en unknown
- 2010-01-27 KR KR1020117021153A patent/KR20110117224A/ko not_active Application Discontinuation
-
2011
- 2011-08-08 IL IL214514A patent/IL214514A0/en unknown
- 2011-08-10 TN TN2011000401A patent/TN2011000401A1/fr unknown
- 2011-08-11 CL CL2011001953A patent/CL2011001953A1/es unknown
- 2011-08-25 CO CO11108508A patent/CO6420362A2/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| MA33040B1 (fr) | 2012-02-01 |
| IL214514A0 (en) | 2011-09-27 |
| CA2751745A1 (en) | 2010-08-19 |
| MX2011008587A (es) | 2011-09-06 |
| WO2010092595A2 (en) | 2010-08-19 |
| CO6420362A2 (es) | 2012-04-16 |
| AU2010213426A1 (en) | 2011-09-01 |
| US20110306037A1 (en) | 2011-12-15 |
| PE20120856A1 (es) | 2012-07-23 |
| EA201171052A1 (ru) | 2012-02-28 |
| TN2011000401A1 (en) | 2013-03-27 |
| EP2396428A2 (en) | 2011-12-21 |
| KR20110117224A (ko) | 2011-10-26 |
| NZ594574A (en) | 2012-06-29 |
| AP2011005866A0 (en) | 2011-10-31 |
| UA99793C2 (ru) | 2012-09-25 |
| EP2396428A4 (en) | 2012-07-25 |
| CL2011001953A1 (es) | 2012-04-20 |
| JP2012517804A (ja) | 2012-08-09 |
| WO2010092595A3 (en) | 2010-10-14 |
| CN102317474A (zh) | 2012-01-11 |
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