SG11202009826PA - Method for large-scale production of lentivirus by using gmp-level serum-free suspension cells - Google Patents
Method for large-scale production of lentivirus by using gmp-level serum-free suspension cellsInfo
- Publication number
- SG11202009826PA SG11202009826PA SG11202009826PA SG11202009826PA SG11202009826PA SG 11202009826P A SG11202009826P A SG 11202009826PA SG 11202009826P A SG11202009826P A SG 11202009826PA SG 11202009826P A SG11202009826P A SG 11202009826PA SG 11202009826P A SG11202009826P A SG 11202009826PA
- Authority
- SG
- Singapore
- Prior art keywords
- lentivirus
- gmp
- scale production
- suspension cells
- free suspension
- Prior art date
Links
- 241000713666 Lentivirus Species 0.000 title 1
- 238000011031 large-scale manufacturing process Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000725 suspension Substances 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16051—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810273392.7A CN110317791A (zh) | 2018-03-29 | 2018-03-29 | Gmp级无血清悬浮细胞大规模生产慢病毒的方法 |
| PCT/CN2019/080215 WO2019184996A1 (fr) | 2018-03-29 | 2019-03-28 | Procédé de production à grande échelle de lentivirus à l'aide d'une suspension cellulaire sans sérum conforme aux bpf |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202009826PA true SG11202009826PA (en) | 2020-11-27 |
Family
ID=68058573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202009826PA SG11202009826PA (en) | 2018-03-29 | 2019-03-28 | Method for large-scale production of lentivirus by using gmp-level serum-free suspension cells |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11845962B2 (fr) |
| EP (1) | EP3778880A4 (fr) |
| JP (2) | JP2021519108A (fr) |
| KR (1) | KR20200136463A (fr) |
| CN (1) | CN110317791A (fr) |
| AU (1) | AU2019241301A1 (fr) |
| SG (1) | SG11202009826PA (fr) |
| WO (1) | WO2019184996A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317791A (zh) * | 2018-03-29 | 2019-10-11 | 西比曼生物科技（香港）有限公司 | Gmp级无血清悬浮细胞大规模生产慢病毒的方法 |
| CN110894494B (zh) * | 2019-11-22 | 2022-09-27 | 广西梧州制药（集团）股份有限公司 | 一种大规模高密度悬浮培养293细胞高产腺病毒的方法 |
| CN113832112A (zh) * | 2020-06-24 | 2021-12-24 | 重庆精准生物技术有限公司 | 一种制备慢病毒的混合方法及混合系统 |
| CN113265425A (zh) * | 2021-05-08 | 2021-08-17 | 苏州博腾生物制药有限公司 | 基于悬浮细胞的car-cd19慢病毒制备工艺 |
| CN114990077A (zh) * | 2022-08-01 | 2022-09-02 | 深圳市先康达生命科学有限公司 | 一种慢病毒包装与纯化方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003039459A2 (fr) | 2001-11-05 | 2003-05-15 | Genvec, Inc. | Procedes de preparation de vecteurs viraux et compositions associees |
| US20030175688A1 (en) | 2002-03-15 | 2003-09-18 | Rukmini Pennathur-Das | Method for the purification and production of oncolytic adenoviruses |
| WO2007095336A2 (fr) | 2006-02-15 | 2007-08-23 | The Immune Response Corporation | Production de vih |
| ES2377356T3 (es) | 2006-04-20 | 2012-03-26 | Wyeth Llc | Procedimiento de purificación para el aislamiento del virus purificado de la estomatitis vesicular de un cultivo celular |
| JP2011507523A (ja) | 2007-12-21 | 2011-03-10 | ワイス・エルエルシー | 遺伝子改変した弱毒化水疱性口内炎ウイルス、組成物およびその使用方法 |
| PL2350268T3 (pl) | 2008-11-03 | 2015-05-29 | Crucell Holland Bv | Sposób wytwarzania wektorów adenowirusowych |
| EP2782997B1 (fr) * | 2011-11-24 | 2018-01-10 | Genethon | Système de production d'un vecteur lentiviral pouvant être mis à l'échelle compatible avec des applications pharmaceutiques industrielles |
| EP2682168A1 (fr) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Dispositif de tirage et métier à filer |
| CN103881984A (zh) * | 2013-12-06 | 2014-06-25 | 深圳市赛百诺基因技术有限公司 | 无血清悬浮细胞生产重组腺病毒的方法及其药品制剂生产方法 |
| FR3014901B1 (fr) | 2013-12-17 | 2017-06-09 | Genethon | Procede de purification de virus ou vecteurs viraux enveloppes |
| CN104371982A (zh) | 2014-10-21 | 2015-02-25 | 武汉维诺赛生物技术有限公司 | 一种慢病毒的纯化方法 |
| EP3054007A1 (fr) | 2015-02-09 | 2016-08-10 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Purification de particules de virus adéno-associés de recombinaison comprenant une étape de purification par affinité immunologique |
| CN107043784A (zh) * | 2016-02-05 | 2017-08-15 | 上海吉凯基因化学技术有限公司 | 一种慢病毒载体的制备方法 |
| CN107523555A (zh) * | 2016-06-22 | 2017-12-29 | 武汉光谷人福生物医药有限公司 | 获得病毒的方法 |
| CN107841482B (zh) * | 2016-09-21 | 2021-07-27 | 上海倍谙基生物科技有限公司 | Mdck细胞无血清悬浮培养技术生产h9亚型流感疫苗 |
| CN106434571A (zh) * | 2016-10-19 | 2017-02-22 | 深圳源兴基因技术有限公司 | 一种wave无血清培养悬浮细胞制备重组腺病毒的方法 |
| CN106474466B (zh) | 2016-12-07 | 2018-04-13 | 申联生物医药（上海）股份有限公司 | 一种口蹄疫疫苗的制备方法 |
| CN107384877B (zh) | 2017-08-18 | 2020-09-08 | 深圳源兴基因技术有限公司 | 一种慢病毒的纯化方法 |
| CN107630037A (zh) | 2017-10-19 | 2018-01-26 | 和元生物技术（上海）股份有限公司 | 一种获得高纯度腺相关病毒载体的纯化工艺 |
| CN110317832B (zh) | 2018-03-28 | 2022-07-05 | 西比曼生物科技（香港）有限公司 | Gmp级规模化制备重组慢病毒载体的纯化制剂的方法 |
| CN110317791A (zh) * | 2018-03-29 | 2019-10-11 | 西比曼生物科技（香港）有限公司 | Gmp级无血清悬浮细胞大规模生产慢病毒的方法 |
-
2018
- 2018-03-29 CN CN201810273392.7A patent/CN110317791A/zh active Pending
-
2019
- 2019-03-28 US US17/041,578 patent/US11845962B2/en active Active
- 2019-03-28 AU AU2019241301A patent/AU2019241301A1/en active Pending
- 2019-03-28 WO PCT/CN2019/080215 patent/WO2019184996A1/fr active Application Filing
- 2019-03-28 EP EP19775992.1A patent/EP3778880A4/fr active Pending
- 2019-03-28 KR KR1020207030733A patent/KR20200136463A/ko unknown
- 2019-03-28 SG SG11202009826PA patent/SG11202009826PA/en unknown
- 2019-03-28 JP JP2021501071A patent/JP2021519108A/ja active Pending
-
2023
- 2023-10-31 US US18/498,879 patent/US20240060053A1/en active Pending
-
2024
- 2024-03-27 JP JP2024051544A patent/JP2024075766A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20240060053A1 (en) | 2024-02-22 |
| KR20200136463A (ko) | 2020-12-07 |
| JP2024075766A (ja) | 2024-06-04 |
| US20210363497A1 (en) | 2021-11-25 |
| US11845962B2 (en) | 2023-12-19 |
| JP2021519108A (ja) | 2021-08-10 |
| EP3778880A4 (fr) | 2021-12-29 |
| CN110317791A (zh) | 2019-10-11 |
| EP3778880A1 (fr) | 2021-02-17 |
| WO2019184996A1 (fr) | 2019-10-03 |
| AU2019241301A1 (en) | 2020-10-22 |
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