SG11201804966VA - Gene cassette for homologous recombination knock-out in yeast cells - Google Patents

Gene cassette for homologous recombination knock-out in yeast cells

Info

Publication number
SG11201804966VA
SG11201804966VA SG11201804966VA SG11201804966VA SG11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA
Authority
SG
Singapore
Prior art keywords
road
international
gda
gene
yeast cells
Prior art date
Application number
SG11201804966VA
Other languages
English (en)
Inventor
Xianzhong Chen
Lihua Zhang
Zheng Xiang
Wei Shen
Li Li
Markus Poetter
Original Assignee
Evonik Degussa China Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa China Co Ltd filed Critical Evonik Degussa China Co Ltd
Publication of SG11201804966VA publication Critical patent/SG11201804966VA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
    • C12R2001/74—Candida tropicalis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11201804966VA 2015-12-17 2015-12-17 Gene cassette for homologous recombination knock-out in yeast cells SG11201804966VA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2015/097675 WO2017101060A1 (en) 2015-12-17 2015-12-17 Gene cassette for homologous recombination knock-out in yeast cells

Publications (1)

Publication Number Publication Date
SG11201804966VA true SG11201804966VA (en) 2018-07-30

Family

ID=59055392

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201804966VA SG11201804966VA (en) 2015-12-17 2015-12-17 Gene cassette for homologous recombination knock-out in yeast cells

Country Status (6)

Country Link
US (1) US20190153475A1 (ja)
EP (1) EP3390640A4 (ja)
JP (1) JP6858191B2 (ja)
CN (1) CN108779470A (ja)
SG (1) SG11201804966VA (ja)
WO (1) WO2017101060A1 (ja)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201804966VA (en) * 2015-12-17 2018-07-30 Evonik Degussa China Co Ltd Gene cassette for homologous recombination knock-out in yeast cells
CN112175984A (zh) * 2020-09-18 2021-01-05 中国科学院深圳先进技术研究院 基于合成基因和酿酒酵母同源重组机制的分子克隆方法
CN114438163B (zh) * 2022-01-27 2024-05-14 深圳瑞德林生物技术有限公司 真菌筛选试剂、筛选方法、试剂盒及应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1515679A (zh) * 1997-09-09 2004-07-28 ���������ﻯѧ���޹�˾ 不含酯酶的酶
WO1999029161A1 (en) * 1997-12-08 1999-06-17 Seminis Vegetable Seeds, Inc. A starchless variety of pisum sativum having elevated levels of sucrose
US20040014198A1 (en) * 2002-05-23 2004-01-22 Craft David L. Non-revertible beta-oxidation blocked candida tropicalis
DK2301949T3 (en) * 2003-05-02 2015-07-20 Cargill Inc Genetically modified yeast species and fermentation methods using genetically modified yeast
MXPA06008670A (es) * 2004-01-30 2007-04-17 Mixis France Sa Generacion de genes recombinantes en saccharomyces cerevisiae.
DE102005048818A1 (de) * 2005-10-10 2007-04-12 Degussa Ag Mikrobiologische Herstellung von 3-Hydroxypropionsäure
DE102009046626A1 (de) * 2009-11-11 2011-05-12 Evonik Degussa Gmbh Candida tropicalis Zellen und deren Verwendung
CN102061296A (zh) * 2010-08-20 2011-05-18 吉林农业大学 一种溶水性vi人胶原蛋白多肽的制备方法
CN103917648B (zh) * 2011-06-20 2016-09-21 阿米卡纳生物技术公司 用于通过同源重组转化和选择酵母转化体的盒和方法
JP6245528B2 (ja) * 2012-01-05 2017-12-13 グリコス・フィンランド・オーワイＧｌｙｋｏｓ Ｆｉｎｌａｎｄ Ｏｙ プロテアーゼ欠損糸状菌細胞及びその使用方法
CN105121624A (zh) * 2012-12-19 2015-12-02 沃德金有限公司 制备脂肪二羧酸的生物学方法
CN103232947B (zh) * 2013-04-12 2015-06-24 天津科技大学 一株耐冷冻面包酵母菌株及其基因无痕敲除方法
WO2015004241A2 (en) * 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
CN105779490A (zh) * 2014-12-16 2016-07-20 北京集智新创科技有限公司 一种och1缺陷型抗cd20四价抗体表达毕赤酵母的构建方法
CN104962488B (zh) * 2015-07-22 2019-03-05 天津大学 一种重组酵母菌株及其构建方法和应用
CN105062908A (zh) * 2015-08-13 2015-11-18 江南大学 一种高产木糖醇的热带假丝酵母基因工程菌及其应用
CN105112313B (zh) * 2015-08-28 2019-04-05 复旦大学 马克思克鲁维酵母营养缺陷型菌株及无痕基因组改造方法
SG11201804966VA (en) * 2015-12-17 2018-07-30 Evonik Degussa China Co Ltd Gene cassette for homologous recombination knock-out in yeast cells
CN108410902B (zh) * 2018-01-24 2021-05-18 齐鲁工业大学 一种新型酿酒酵母表达体系及其构建方法
CN109082444A (zh) * 2018-07-30 2018-12-25 惠州卫生职业技术学院 一种毕赤酵母高效基因敲除方法
CN110452865B (zh) * 2019-08-15 2021-05-28 江南大学 一种产酪醇的重组大肠杆菌及其构建方法和应用
CN112941119B (zh) * 2021-01-22 2022-08-30 江南大学 一种提高酿酒酵母工程菌脂肪酸乙酯产量的方法

Also Published As

Publication number Publication date
CN108779470A (zh) 2018-11-09
JP6858191B2 (ja) 2021-04-14
EP3390640A1 (en) 2018-10-24
US20190153475A1 (en) 2019-05-23
WO2017101060A1 (en) 2017-06-22
EP3390640A4 (en) 2019-05-22
JP2018537109A (ja) 2018-12-20

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