SG11201700023WA - New undifferentiated stem cell removal and myocardial purification and refinement culture medium - Google Patents

New undifferentiated stem cell removal and myocardial purification and refinement culture medium

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Publication number
SG11201700023WA
SG11201700023WA SG11201700023WA SG11201700023WA SG11201700023WA SG 11201700023W A SG11201700023W A SG 11201700023WA SG 11201700023W A SG11201700023W A SG 11201700023WA SG 11201700023W A SG11201700023W A SG 11201700023WA SG 11201700023W A SG11201700023W A SG 11201700023WA
Authority
SG
Singapore
Prior art keywords
refinement
culture medium
stem cell
undifferentiated stem
cell removal
Prior art date
Application number
SG11201700023WA
Other languages
English (en)
Inventor
Keiichi Fukuda
Jun Fujita
Shugo Tohyama
Original Assignee
Heartseed Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Heartseed Inc filed Critical Heartseed Inc
Publication of SG11201700023WA publication Critical patent/SG11201700023WA/en

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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081Purging biological preparations of unwanted cells
    • C12N5/0087Purging against subsets of blood cells, e.g. purging alloreactive T cells
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0623Stem cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657Cardiomyocytes; Heart cells
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    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Cardiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201700023WA 2014-07-16 2015-07-16 New undifferentiated stem cell removal and myocardial purification and refinement culture medium SG11201700023WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2014146283 2014-07-16
PCT/JP2015/071048 WO2016010165A1 (ja) 2014-07-16 2015-07-16 新規未分化幹細胞除去および心筋純化精製培地

Publications (1)

Publication Number Publication Date
SG11201700023WA true SG11201700023WA (en) 2017-02-27

Family

ID=55078650

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201700023WA SG11201700023WA (en) 2014-07-16 2015-07-16 New undifferentiated stem cell removal and myocardial purification and refinement culture medium

Country Status (12)

Country Link
US (1) US20170327787A1 (ru)
EP (1) EP3170894B1 (ru)
JP (1) JP6333378B2 (ru)
KR (1) KR102241348B1 (ru)
CN (1) CN107075475B (ru)
AU (1) AU2015290571B2 (ru)
BR (1) BR112016030723B8 (ru)
CA (1) CA2954242C (ru)
IL (1) IL249813B (ru)
RU (1) RU2728188C2 (ru)
SG (1) SG11201700023WA (ru)
WO (1) WO2016010165A1 (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114591895A (zh) * 2022-04-12 2022-06-07 澳门大学 一种诱导干细胞分化为心肌细胞的方法及其培养基组合

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3040637C (en) * 2016-10-17 2023-02-28 Keio University Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells
JP2018093823A (ja) 2016-12-15 2018-06-21 Heartseed株式会社 未分化幹細胞除去剤及び未分化幹細胞除去方法
CN110494559B (zh) * 2017-03-28 2023-10-31 味之素株式会社 不分化地维持用培养基添加剂
KR101994035B1 (ko) * 2018-02-14 2019-06-27 (주) 넥셀 세포 분화 및 성숙을 통한 인간 전분화능 줄기세포 유래 기능성 심근세포의 제조 방법
WO2020080270A1 (ja) * 2018-10-15 2020-04-23 公立大学法人横浜市立大学 栄養組成物
WO2021187602A1 (ja) * 2020-03-19 2021-09-23 国立大学法人京都大学 心筋細胞の精製方法
CN111500532B (zh) * 2020-04-14 2024-10-11 上海中医大生物科技有限公司 心肌细胞诱导和生长培养基
CN111621470B (zh) * 2020-06-08 2023-06-27 广东源心再生医学有限公司 一种高效低毒的心肌纯化培养基及方法
WO2024173685A1 (en) * 2023-02-15 2024-08-22 The Board Of Trustees Of The Leland Stanford Junior University Metabolic selection for glycogen-storing cells in vitro

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2002024875A1 (ja) * 2000-09-22 2004-01-29 北海道ティー・エル・オー株式会社 正常ヒト成熟肝細胞用培養液
DE10226455A1 (de) * 2002-06-13 2003-12-24 Ruediger Alt Verfahren zur Kultivierung von Säugerzellen mit verminderter Hemmstoffproduktion
SI1720979T1 (sl) * 2004-03-01 2007-12-31 Ares Trading Sa Uporaba medija za celiäśno kulturo brez seruma zaizdelavo il-18bp pri celicah sesalcev
US8623649B2 (en) 2004-08-27 2014-01-07 Daiichi Sankyo Company, Limited Method of selecting a cardiomyoctye using intracellular mitochondria as an indicator
US9115342B2 (en) * 2006-01-31 2015-08-25 Daiichi Sankyo Company Method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
RU2340177C1 (ru) * 2007-05-14 2008-12-10 Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ) Способ получения эмбрионов свиней in vitro
RU2467066C2 (ru) 2007-07-31 2012-11-20 Дайити Санкио Комани, Лимитед Способ конструирования массы миокардиальных клеток и применение массы миокардиальных клеток
MX2010003622A (es) * 2007-10-12 2010-04-14 Hoffmann La Roche Expresion proteinica a partir de acidos nucleicos multiples.
KR101656761B1 (ko) 2009-03-30 2016-09-12 다이이찌 산쿄 가부시키가이샤 다능성 줄기 세포 및 심근 세포 이외의 분화성 세포에 대해 세포사멸을 유도하는 방법
KR20180114966A (ko) * 2009-08-11 2018-10-19 제넨테크, 인크. 글루타민-비함유 세포 배양 배지에서의 단백질의 생성
AU2010312410A1 (en) 2009-10-30 2012-05-31 Daiichi Sankyo Company, Limited Method for measurement of fluorescence intensity of voltage-sensitive fluorescent dye
US9657273B2 (en) * 2012-10-12 2017-05-23 Industry-University Cooperation Foundation Sogang University Method for suppressing teratoma formation via selective cell death induction in undifferentiated human-induced pluripotent stem cells
WO2015148515A1 (en) * 2014-03-24 2015-10-01 Biogen Ma Inc. Methods for overcoming glutamine deprivation during mammalian cell culture

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114591895A (zh) * 2022-04-12 2022-06-07 澳门大学 一种诱导干细胞分化为心肌细胞的方法及其培养基组合

Also Published As

Publication number Publication date
CA2954242C (en) 2024-02-13
CN107075475A (zh) 2017-08-18
CN107075475B (zh) 2021-04-27
BR112016030723A2 (ru) 2017-08-22
EP3170894B1 (en) 2020-04-01
CA2954242A1 (en) 2016-01-21
RU2017104838A3 (ru) 2018-10-24
EP3170894A4 (en) 2018-01-24
RU2728188C2 (ru) 2020-07-28
IL249813A0 (en) 2017-03-30
US20170327787A1 (en) 2017-11-16
AU2015290571B2 (en) 2021-03-04
IL249813B (en) 2019-03-31
AU2015290571A1 (en) 2017-01-19
KR20170031152A (ko) 2017-03-20
RU2017104838A (ru) 2018-08-16
WO2016010165A1 (ja) 2016-01-21
EP3170894A1 (en) 2017-05-24
KR102241348B1 (ko) 2021-04-15
BR112016030723B8 (pt) 2024-02-27
JPWO2016010165A1 (ja) 2017-04-27
JP6333378B2 (ja) 2018-05-30
BR112016030723B1 (pt) 2024-01-09

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