BR112016030723B8 - Meios de cultura de células, método para indução da morte celular de células-tronco indiferenciadas e métodos para seleção de cardiomiócitos - Google Patents

Meios de cultura de células, método para indução da morte celular de células-tronco indiferenciadas e métodos para seleção de cardiomiócitos

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Publication number
BR112016030723B8
BR112016030723B8 BR112016030723A BR112016030723A BR112016030723B8 BR 112016030723 B8 BR112016030723 B8 BR 112016030723B8 BR 112016030723 A BR112016030723 A BR 112016030723A BR 112016030723 A BR112016030723 A BR 112016030723A BR 112016030723 B8 BR112016030723 B8 BR 112016030723B8
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Brazil
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cell culture
cardiomyocytes
culture medium
stem cells
undifferentiated stem
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BR112016030723A
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English (en)
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BR112016030723B1 (pt
BR112016030723A2 (pt
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Jun Fujita
Keiichi Fukuda
Shugo Tohyama
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Heartseed Inc
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Publication of BR112016030723A2 publication Critical patent/BR112016030723A2/pt
Publication of BR112016030723B1 publication Critical patent/BR112016030723B1/pt
Publication of BR112016030723B8 publication Critical patent/BR112016030723B8/pt

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  • Health & Medical Sciences (AREA)
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Abstract

remoção de células-tronco indiferenciadas novas e meio de cultura para purificação e refinamento de cardiomiócitos. a presente invenção tem como objetico encontrar novas condições para possibilitar a indução da morte celular de não-cardiomiócitos ou células-tronco indiferenciadas de forma mais completa e a seleção apenas de cardiomióticos. para atingir este objetivo, são oferecidos no presente pedido: um meio de cultura de células para uso na indução da morte celular de células-tronco indiferenciadas, onde o meio de cultura de células é livre de glutamina no perfil de aminoácidos; e também um método para induzir a morte celular de não-cardiomiócitos sendo feita uma cultura de células no referido meio de cultura de células. são ainda oferecidos neste pedido: um meio de cultura de células para uso na seleção de cardiomiócitos, onde o meio de cultura de células é suplementado com lactato, piruvato ou um ácido graxo, livre de açúcar, e livre de glutamina no perfil de aminoácidos; e também um método para selecionar cardiomiócitos por cultivo de uma mistura de cardiomiócitos e não-cardiomiócitos no referido meio de cultura de células.
BR112016030723A 2014-07-16 2015-07-16 Meios de cultura de células, método para indução da morte celular de células-tronco indiferenciadas e métodos para seleção de cardiomiócitos BR112016030723B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014146283 2014-07-16
JP2014-146283 2014-07-16
PCT/JP2015/071048 WO2016010165A1 (ja) 2014-07-16 2015-07-16 新規未分化幹細胞除去および心筋純化精製培地

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BR112016030723A2 BR112016030723A2 (pt) 2017-08-22
BR112016030723B1 BR112016030723B1 (pt) 2024-01-09
BR112016030723B8 true BR112016030723B8 (pt) 2024-02-27

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US (1) US20170327787A1 (pt)
EP (1) EP3170894B1 (pt)
JP (1) JP6333378B2 (pt)
KR (1) KR102241348B1 (pt)
CN (1) CN107075475B (pt)
AU (1) AU2015290571B2 (pt)
BR (1) BR112016030723B8 (pt)
CA (1) CA2954242C (pt)
IL (1) IL249813B (pt)
RU (1) RU2728188C2 (pt)
SG (1) SG11201700023WA (pt)
WO (1) WO2016010165A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201903254PA (en) * 2016-10-17 2019-05-30 Univ Keio Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells
JP2018093823A (ja) 2016-12-15 2018-06-21 Ｈｅａｒｔｓｅｅｄ株式会社 未分化幹細胞除去剤及び未分化幹細胞除去方法
CN110494559B (zh) * 2017-03-28 2023-10-31 味之素株式会社 不分化地维持用培养基添加剂
KR101994035B1 (ko) * 2018-02-14 2019-06-27 (주) 넥셀 세포 분화 및 성숙을 통한 인간 전분화능 줄기세포 유래 기능성 심근세포의 제조 방법
EP3868869A4 (en) * 2018-10-15 2022-08-03 Public University Corporation Yokohama City University NUTRITIONAL COMPOSITION
WO2021187602A1 (ja) * 2020-03-19 2021-09-23 国立大学法人京都大学 心筋細胞の精製方法
CN111500532A (zh) * 2020-04-14 2020-08-07 上海产业技术研究院 心肌细胞诱导和生长培养基
CN111621470B (zh) * 2020-06-08 2023-06-27 广东源心再生医学有限公司 一种高效低毒的心肌纯化培养基及方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2002024875A1 (ja) * 2000-09-22 2004-01-29 北海道ティー・エル・オー株式会社 正常ヒト成熟肝細胞用培養液
DE10226455A1 (de) * 2002-06-13 2003-12-24 Ruediger Alt Verfahren zur Kultivierung von Säugerzellen mit verminderter Hemmstoffproduktion
ME01218B (me) * 2004-03-01 2013-06-20 Ares Trading Sa Upotreba podloge za ćelijsku strukturu bez sadrzaja seruma za produkciju il-18bp u ćelijama sisara
KR20120088865A (ko) 2004-08-27 2012-08-08 각고호우징 게이오기주크 세포내 미토콘드리아를 지표로 이용하는 심근 세포의 선별 방법
CA2640644C (en) * 2006-01-31 2013-11-26 Asubio Pharma Co., Ltd. A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
RU2340177C1 (ru) * 2007-05-14 2008-12-10 Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ) Способ получения эмбрионов свиней in vitro
CA2690610C (en) 2007-07-31 2023-03-28 Asubio Pharma Co., Ltd. Method of constructing masses of myocardial cells and use of the myocardial cell mass
WO2009046978A1 (en) * 2007-10-12 2009-04-16 F. Hoffmann-La Roche Ag Protein expression from multiple nucleic acids
WO2010114136A1 (ja) 2009-03-30 2010-10-07 第一三共株式会社 多能性幹細胞及び心筋細胞以外の分化細胞に対する細胞死誘導方法
SG178358A1 (en) * 2009-08-11 2012-03-29 Genentech Inc Production of proteins in glutamine-free cell culture media
AU2010312410A1 (en) 2009-10-30 2012-05-31 Daiichi Sankyo Company, Limited Method for measurement of fluorescence intensity of voltage-sensitive fluorescent dye
US9657273B2 (en) * 2012-10-12 2017-05-23 Industry-University Cooperation Foundation Sogang University Method for suppressing teratoma formation via selective cell death induction in undifferentiated human-induced pluripotent stem cells
US11124760B2 (en) * 2014-03-24 2021-09-21 Biogen Ma Inc. Methods for overcoming glutamine deprivation during mammalian cell culture

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IL249813A0 (en) 2017-03-30
CA2954242C (en) 2024-02-13
RU2728188C2 (ru) 2020-07-28
EP3170894B1 (en) 2020-04-01
AU2015290571A1 (en) 2017-01-19
CN107075475B (zh) 2021-04-27
IL249813B (en) 2019-03-31
AU2015290571B2 (en) 2021-03-04
CA2954242A1 (en) 2016-01-21
JPWO2016010165A1 (ja) 2017-04-27
BR112016030723B1 (pt) 2024-01-09
CN107075475A (zh) 2017-08-18
US20170327787A1 (en) 2017-11-16
KR20170031152A (ko) 2017-03-20
RU2017104838A3 (pt) 2018-10-24
EP3170894A1 (en) 2017-05-24
RU2017104838A (ru) 2018-08-16
EP3170894A4 (en) 2018-01-24
SG11201700023WA (en) 2017-02-27
JP6333378B2 (ja) 2018-05-30
WO2016010165A1 (ja) 2016-01-21
KR102241348B1 (ko) 2021-04-15
BR112016030723A2 (pt) 2017-08-22

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Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B25G Requested change of headquarter approved

Owner name: HEARTSEED INC. (JP)

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 16/07/2015, OBSERVADAS AS CONDICOES LEGAIS

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: REFERENTE A RPI 2766 DE 09/01/2024, QUANTO AO ITEM (73) ENDERECO DO TITULAR.