SG11201504694VA - Method for producing a recombinant protein of interest - Google Patents

Method for producing a recombinant protein of interest

Info

Publication number
SG11201504694VA
SG11201504694VA SG11201504694VA SG11201504694VA SG11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA
Authority
SG
Singapore
Prior art keywords
interest
producing
recombinant protein
recombinant
protein
Prior art date
Application number
SG11201504694VA
Other languages
English (en)
Inventor
Maria Reitmeir
Rainer Schneider
Bernhard Auer
Original Assignee
Sandoz Ag
Boehringer Ingelheim Rcv Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz Ag, Boehringer Ingelheim Rcv Gmbh filed Critical Sandoz Ag
Publication of SG11201504694VA publication Critical patent/SG11201504694VA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11201504694VA 2012-12-19 2013-12-19 Method for producing a recombinant protein of interest SG11201504694VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12198006.4A EP2746390A1 (fr) 2012-12-19 2012-12-19 Procédé de production d'une protéine recombinante d'intérêt
PCT/EP2013/077464 WO2014096239A1 (fr) 2012-12-19 2013-12-19 Procédé de production d'une protéine recombinante d'intérêt

Publications (1)

Publication Number Publication Date
SG11201504694VA true SG11201504694VA (en) 2015-07-30

Family

ID=47358396

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201504694VA SG11201504694VA (en) 2012-12-19 2013-12-19 Method for producing a recombinant protein of interest

Country Status (9)

Country Link
US (1) US9677107B2 (fr)
EP (2) EP2746390A1 (fr)
JP (2) JP6285459B2 (fr)
KR (1) KR102141353B1 (fr)
CN (1) CN105189743B (fr)
AU (1) AU2013366602B2 (fr)
CA (1) CA2894941C (fr)
SG (1) SG11201504694VA (fr)
WO (1) WO2014096239A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104558100A (zh) * 2015-02-04 2015-04-29 吉林农业大学 包涵体的预处理方法
EP3256597B1 (fr) * 2015-02-09 2018-11-14 Sandoz AG Procédé de production d'une protéine recombinante d'intérêt
EP3268384B1 (fr) 2015-03-10 2021-11-03 Merck Sharp & Dohme Corp. Procédé de préparation d'insuline recombinante par microfiltration
MX2019001751A (es) * 2016-08-10 2019-06-10 Spiber Inc Metodo de produccion para agregado de proteina recombinante insoluble.
US10738338B2 (en) 2016-10-18 2020-08-11 The Research Foundation for the State University Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
CN109957591A (zh) * 2017-12-22 2019-07-02 中国医学科学院医药生物技术研究所 双靶向配体化力达霉素dtll的制备工艺改进和质量标准制定
DE102018200602A1 (de) * 2018-01-15 2019-07-18 Technische Universität München Biologische Synthese von Aminosäureketten zur Herstellung von Peptiden und Proteinen
WO2019143193A1 (fr) 2018-01-19 2019-07-25 주식회사 펩진 Homologue de fusion n-terminal pour la production d'un polypeptide recombinant, et procédé de production d'un polypeptide recombinant utilisant ce dernier
CN110343183B (zh) * 2018-04-03 2023-05-12 点斗基因科技(南京)有限公司 一种重组表达载体及其构建方法和应用
EP3904525A1 (fr) 2020-04-27 2021-11-03 Kutzner, Christoph Polypeptides de fusion pour la production de peptides cibles
EP4265636A1 (fr) 2022-04-19 2023-10-25 mk2 Biotechnologies GmbH Préparation de peptides et de protéines cibles
CN114958806A (zh) * 2022-06-14 2022-08-30 中国水产科学研究院淡水渔业研究中心 一种鲤lpl1重组蛋白及其制备方法
CN116042585B (zh) * 2022-07-11 2023-10-03 无锡佰翱得生物科学股份有限公司 SortaseA酶作为蛋白水解酶在蛋白纯化领域中的应用
KR20240064367A (ko) * 2022-11-04 2024-05-13 주식회사 대웅제약 TGF-β3 단백질의 정제방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL203708B1 (pl) * 1999-08-09 2009-11-30 Sandoz Ag Cząsteczka kwasu nukleinowego kodująca białko fuzyjne, wektor ekspresyjny zawierający tę cząsteczkę oraz komórka gospodarza bakteryjnego zawierająca ten wektor
US6936455B1 (en) 1999-08-09 2005-08-30 Sandoz Ag Production of heterologous proteins using an Npro autoprotease of a pestivirus and inclusion bodies
WO2006113958A2 (fr) 2005-04-26 2006-11-02 Sandoz Ag Ligands d'affinités
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
EP2746391A1 (fr) * 2012-12-19 2014-06-25 Sandoz Ag Procédé de production d'une protéine recombinante d'intérêt

Also Published As

Publication number Publication date
JP6285459B2 (ja) 2018-02-28
CA2894941A1 (fr) 2014-06-26
EP2935576B1 (fr) 2018-10-31
JP6554514B2 (ja) 2019-07-31
EP2935576A1 (fr) 2015-10-28
CN105189743B (zh) 2020-02-18
US9677107B2 (en) 2017-06-13
JP2016500273A (ja) 2016-01-12
EP2746390A1 (fr) 2014-06-25
JP2018023380A (ja) 2018-02-15
KR102141353B1 (ko) 2020-08-06
CA2894941C (fr) 2024-02-27
CN105189743A (zh) 2015-12-23
KR20150099741A (ko) 2015-09-01
AU2013366602A1 (en) 2015-06-18
US20140170702A1 (en) 2014-06-19
AU2013366602B2 (en) 2019-05-30
WO2014096239A1 (fr) 2014-06-26

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