SG11201504694VA - Method for producing a recombinant protein of interest - Google Patents

Method for producing a recombinant protein of interest

Info

Publication number
SG11201504694VA
SG11201504694VA SG11201504694VA SG11201504694VA SG11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA SG 11201504694V A SG11201504694V A SG 11201504694VA
Authority
SG
Singapore
Prior art keywords
interest
producing
recombinant protein
recombinant
protein
Prior art date
Application number
SG11201504694VA
Inventor
Maria Reitmeir
Rainer Schneider
Bernhard Auer
Original Assignee
Sandoz Ag
Boehringer Ingelheim Rcv Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz Ag, Boehringer Ingelheim Rcv Gmbh filed Critical Sandoz Ag
Publication of SG11201504694VA publication Critical patent/SG11201504694VA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11201504694VA 2012-12-19 2013-12-19 Method for producing a recombinant protein of interest SG11201504694VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12198006.4A EP2746390A1 (en) 2012-12-19 2012-12-19 Method for producing a recombinant protein of interest
PCT/EP2013/077464 WO2014096239A1 (en) 2012-12-19 2013-12-19 Method for producing a recombinant protein of interest

Publications (1)

Publication Number Publication Date
SG11201504694VA true SG11201504694VA (en) 2015-07-30

Family

ID=47358396

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201504694VA SG11201504694VA (en) 2012-12-19 2013-12-19 Method for producing a recombinant protein of interest

Country Status (9)

Country Link
US (1) US9677107B2 (en)
EP (2) EP2746390A1 (en)
JP (2) JP6285459B2 (en)
KR (1) KR102141353B1 (en)
CN (1) CN105189743B (en)
AU (1) AU2013366602B2 (en)
CA (1) CA2894941C (en)
SG (1) SG11201504694VA (en)
WO (1) WO2014096239A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104558100A (en) * 2015-02-04 2015-04-29 吉林农业大学 Inclusion body pretreatment method
EP3256597B1 (en) * 2015-02-09 2018-11-14 Sandoz AG Method for producing a recombinant protein of interest
EP3268384B1 (en) 2015-03-10 2021-11-03 Merck Sharp & Dohme Corp. Process for preparing recombinant insulin using microfiltration
CN109661472A (en) * 2016-08-10 2019-04-19 丝芭博株式会社 The manufacturing method of insoluble recombinant protein agglutination body
CN109957591A (en) * 2017-12-22 2019-07-02 中国医学科学院医药生物技术研究所 The preparation process amelioration and quality standard of double targeting ligand lidamycin DTLL is formulated
DE102018200602A1 (en) * 2018-01-15 2019-07-18 Technische Universität München Biological synthesis of amino acid chains for the production of peptides and proteins
WO2019143193A1 (en) 2018-01-19 2019-07-25 주식회사 펩진 N-terminal fusion partner for producing recombinant polypeptide, and method for producing recombinant polypeptide using same
CN110343183B (en) * 2018-04-03 2023-05-12 点斗基因科技(南京)有限公司 Recombinant expression vector and construction method and application thereof
EP3904525A1 (en) 2020-04-27 2021-11-03 Kutzner, Christoph Fusion polypeptides for target peptide production
EP4265636A1 (en) 2022-04-19 2023-10-25 mk2 Biotechnologies GmbH Preparation of target peptides and proteins
CN114958806A (en) * 2022-06-14 2022-08-30 中国水产科学研究院淡水渔业研究中心 Carp LPL1 recombinant protein and preparation method thereof
CN116042585B (en) * 2022-07-11 2023-10-03 无锡佰翱得生物科学股份有限公司 Application of Sortase A enzyme as proteolytic enzyme in field of protein purification
KR20240064367A (en) * 2022-11-04 2024-05-13 주식회사 대웅제약 Method for purifying protein of TGF-β3

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60029968T2 (en) * 1999-08-09 2006-12-21 Sandoz Ag PROTEIN PRODUCTION
CN1230542C (en) * 1999-08-09 2005-12-07 山德士股份公司 Production of proteins by autoproteolytic cleavage
EP1874933B1 (en) * 2005-04-26 2014-11-19 Sandoz Ag Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
EP2746391A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest

Also Published As

Publication number Publication date
EP2746390A1 (en) 2014-06-25
CN105189743B (en) 2020-02-18
WO2014096239A1 (en) 2014-06-26
KR20150099741A (en) 2015-09-01
CA2894941A1 (en) 2014-06-26
JP2016500273A (en) 2016-01-12
CA2894941C (en) 2024-02-27
EP2935576A1 (en) 2015-10-28
JP6285459B2 (en) 2018-02-28
EP2935576B1 (en) 2018-10-31
JP6554514B2 (en) 2019-07-31
AU2013366602A1 (en) 2015-06-18
CN105189743A (en) 2015-12-23
KR102141353B1 (en) 2020-08-06
AU2013366602B2 (en) 2019-05-30
JP2018023380A (en) 2018-02-15
US20140170702A1 (en) 2014-06-19
US9677107B2 (en) 2017-06-13

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