SE468450B - Saett att bestaemma antikroppar mot sporozoiter fraan p. falciparum i humant blod - Google Patents
Saett att bestaemma antikroppar mot sporozoiter fraan p. falciparum i humant blodInfo
- Publication number
- SE468450B SE468450B SE8705074A SE8705074A SE468450B SE 468450 B SE468450 B SE 468450B SE 8705074 A SE8705074 A SE 8705074A SE 8705074 A SE8705074 A SE 8705074A SE 468450 B SE468450 B SE 468450B
- Authority
- SE
- Sweden
- Prior art keywords
- enzyme
- conjugate
- reaction
- asn
- polypeptide
- Prior art date
Links
- 210000004369 blood Anatomy 0.000 title claims description 15
- 239000008280 blood Substances 0.000 title claims description 15
- 241000223960 Plasmodium falciparum Species 0.000 title claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 32
- 108090000790 Enzymes Proteins 0.000 claims description 32
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 24
- 229920001184 polypeptide Polymers 0.000 claims description 14
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 14
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 230000002255 enzymatic effect Effects 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 150000004676 glycans Chemical class 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 229920001282 polysaccharide Polymers 0.000 claims description 3
- 239000005017 polysaccharide Substances 0.000 claims description 3
- 210000003046 sporozoite Anatomy 0.000 claims description 3
- 229920000936 Agarose Polymers 0.000 claims description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims description 2
- 108010008038 Synthetic Vaccines Proteins 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 238000002405 diagnostic procedure Methods 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims 2
- SWLOHUMCUDRTCL-ZLUOBGJFSA-N Asn-Ala-Asn Chemical compound C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N SWLOHUMCUDRTCL-ZLUOBGJFSA-N 0.000 claims 1
- 230000009849 deactivation Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 230000001900 immune effect Effects 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 239000000562 conjugate Substances 0.000 description 22
- 210000002966 serum Anatomy 0.000 description 14
- 239000000243 solution Substances 0.000 description 13
- 201000004792 malaria Diseases 0.000 description 11
- 102000003992 Peroxidases Human genes 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 8
- 238000011161 development Methods 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 5
- 108040007629 peroxidase activity proteins Proteins 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000021615 conjugation Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000007974 sodium acetate buffer Substances 0.000 description 3
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 108010074605 gamma-Globulins Proteins 0.000 description 2
- 239000011491 glass wool Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 101710117490 Circumsporozoite protein Proteins 0.000 description 1
- -1 E-P compound Chemical class 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 102000005840 alpha-Galactosidase Human genes 0.000 description 1
- 108010030291 alpha-Galactosidase Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000011841 epidemiological investigation Methods 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 125000000896 monocarboxylic acid group Chemical group 0.000 description 1
- 150000007530 organic bases Chemical group 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000000863 peptide conjugate Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/947—Microorganisms using protozoa
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/828—Protein A
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT22817/86A IT1198233B (it) | 1986-12-23 | 1986-12-23 | Metodo per la determinazione di anticorpi antisporozoita di p. falciparum nel sangue umano |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE8705074D0 SE8705074D0 (sv) | 1987-12-18 |
| SE8705074L SE8705074L (sv) | 1988-06-24 |
| SE468450B true SE468450B (sv) | 1993-01-18 |
Family
ID=11200776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8705074A SE468450B (sv) | 1986-12-23 | 1987-12-18 | Saett att bestaemma antikroppar mot sporozoiter fraan p. falciparum i humant blod |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US4977079A (it) |
| AR (1) | AR241963A1 (it) |
| AT (1) | AT397581B (it) |
| BE (1) | BE1000850A3 (it) |
| CA (1) | CA1299101C (it) |
| CH (1) | CH677032A5 (it) |
| DE (1) | DE3743903A1 (it) |
| ES (1) | ES2005766A6 (it) |
| FR (1) | FR2608767B1 (it) |
| GB (1) | GB2199140B (it) |
| GR (1) | GR871991B (it) |
| IT (1) | IT1198233B (it) |
| NL (1) | NL8703102A (it) |
| OA (1) | OA08792A (it) |
| SE (1) | SE468450B (it) |
| ZA (1) | ZA879621B (it) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1217582B (it) * | 1988-05-13 | 1990-03-30 | Eniricerche Spa | Metodo immunoenzimatico in fase omogenea per la determinazione di anticorpi antisporozoita di plasmodium falciparum nel sangue umano |
| IT1226712B (it) * | 1988-08-05 | 1991-02-05 | Eniricerche Spa | Metodo immunoenzimatico elisa monopiastra con inibizione competitiva per la rivelazione di anticorpi antisporozoitari di plasmodium falciparum. |
| US5231168A (en) * | 1988-09-16 | 1993-07-27 | Statens Seruminstitut | Malaria antigen |
| DE69014150T2 (de) * | 1989-05-19 | 1995-05-11 | Eniricerche Spa | Immunogen-Verbindungen und deren Benutzung in der Herstellung von genetisch unbeschränkten synthetischen Impfstoffen und bei der immunoenzymatischer Bestimmung von antisporozoiden Antikörpern von Plasmodium malariae. |
| US5124141A (en) * | 1990-06-14 | 1992-06-23 | Flow Incorporated | Method for diagnosing malaria |
| US5830772A (en) * | 1995-09-08 | 1998-11-03 | United Microelectronicscorp. | Method for fabricating isolating regions for buried conductors |
| US6514731B1 (en) | 1996-05-24 | 2003-02-04 | Chiron Corporation | Methods for the preparation of hepatitis C virus multiple copy epitope fusion antigens |
| US6153394A (en) * | 1998-09-18 | 2000-11-28 | Board Of Trustees Operating Michigan State University | Immunoassay for equine protozoal myeloencephalitis in horses |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2322533C2 (de) * | 1972-11-06 | 1986-08-28 | Pharmacia AB, Uppsala | Verfahren zum Binden von Immunoglobulin und Hilfsmittel zur Durchführung des Verfahrens |
| US3966898A (en) * | 1973-06-28 | 1976-06-29 | Pharmacia Diagnostics Ab | Method and reagent for determining immunologic materials |
| EP0111216A3 (en) * | 1980-03-31 | 1985-01-16 | Takeda Chemical Industries, Ltd. | Method for enzyme immunoassay and peptide-enzyme conjugate, its lyophilizate, antibody and kit therefor |
| US4659678A (en) * | 1982-09-29 | 1987-04-21 | Serono Diagnostics Limited | Immunoassay of antigens |
| US4591552A (en) * | 1982-09-29 | 1986-05-27 | New York Blood Center, Inc. | Detection of hepatitis B surface antigen (or antibody to same) with labeled synthetic peptide |
| KR910005702B1 (ko) * | 1982-12-27 | 1991-08-02 | 앵스띠뛰 빠스뙤르 | 말라리아 기생충에 대한 방어항체를 유도하는 폴리펩티드 단편을 함유한 면역원 조성물의 제조방법 |
| DE3346795A1 (de) * | 1983-12-23 | 1985-07-04 | E. Prof. Dr. 3550 Marburg Geyer | Enzymimmunoassay und entsprechendes diagnostikum |
| US4707357A (en) * | 1984-06-26 | 1987-11-17 | The United States Of America As Represented By The Secretary Of The Army | Immunologically active peptides capable of inducing immunization against malaria and genes encoding therefor |
| ZA855232B (en) * | 1984-07-23 | 1986-02-26 | Univ New York | Protective peptide antigen corresponding to plasmodium falciparum circumsporozoite protein |
| NZ213303A (en) * | 1984-09-12 | 1988-09-29 | Univ New York | Plasmodium protein, dna sequence and vaccine |
| IT1187710B (it) * | 1985-07-25 | 1987-12-23 | Eniricerche Spa | Composizione peptidica utile per la preparazione di vaccini per la malaria e di kit diagnostici per la determinazione di affezioni malariche |
| FR2589062B1 (fr) * | 1985-10-28 | 1990-05-04 | Inst Nat Sante Rech Med | Antigenes obtenus a partir de la phase intraerythrocytaire de plasmodium falciparum, leur purification, leur dosage ainsi que celui de leurs anticorps, et vaccins contre le paludisme les renfermant |
| IT1198225B (it) * | 1986-12-04 | 1988-12-21 | Eniricerche Spa | Polipeptidi immunologicamente attivi utili per la preparazione di vaccini antimalaria e di kits diagnostici per la determinazione di anticorpi antisporozoita |
-
1986
- 1986-12-23 IT IT22817/86A patent/IT1198233B/it active
-
1987
- 1987-12-17 US US07/134,229 patent/US4977079A/en not_active Expired - Fee Related
- 1987-12-18 SE SE8705074A patent/SE468450B/sv unknown
- 1987-12-21 CH CH5001/87A patent/CH677032A5/it not_active IP Right Cessation
- 1987-12-22 GR GR871991A patent/GR871991B/el unknown
- 1987-12-22 AT AT0338987A patent/AT397581B/de not_active IP Right Cessation
- 1987-12-22 NL NL8703102A patent/NL8703102A/nl not_active Application Discontinuation
- 1987-12-22 ZA ZA879621A patent/ZA879621B/xx unknown
- 1987-12-23 AR AR87309697A patent/AR241963A1/es active
- 1987-12-23 CA CA000555290A patent/CA1299101C/en not_active Expired - Lifetime
- 1987-12-23 DE DE19873743903 patent/DE3743903A1/de active Granted
- 1987-12-23 OA OA59253A patent/OA08792A/xx unknown
- 1987-12-23 ES ES8800193A patent/ES2005766A6/es not_active Expired
- 1987-12-23 GB GB8729975A patent/GB2199140B/en not_active Expired - Lifetime
- 1987-12-23 BE BE8701477A patent/BE1000850A3/fr not_active IP Right Cessation
- 1987-12-23 FR FR878718090A patent/FR2608767B1/fr not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| GR871991B (en) | 1988-03-23 |
| GB2199140B (en) | 1990-08-29 |
| FR2608767A1 (fr) | 1988-06-24 |
| AT397581B (de) | 1994-05-25 |
| OA08792A (en) | 1989-03-31 |
| AR241963A1 (es) | 1993-01-29 |
| SE8705074L (sv) | 1988-06-24 |
| BE1000850A3 (fr) | 1989-04-18 |
| NL8703102A (nl) | 1988-07-18 |
| DE3743903A1 (de) | 1988-07-07 |
| ZA879621B (en) | 1988-06-17 |
| GB8729975D0 (en) | 1988-02-03 |
| CA1299101C (en) | 1992-04-21 |
| CH677032A5 (it) | 1991-03-28 |
| US4977079A (en) | 1990-12-11 |
| ATA338987A (de) | 1993-09-15 |
| GB2199140A (en) | 1988-06-29 |
| FR2608767B1 (fr) | 1991-04-05 |
| IT8622817A0 (it) | 1986-12-23 |
| ES2005766A6 (es) | 1989-03-16 |
| IT1198233B (it) | 1988-12-21 |
| DE3743903C2 (it) | 1990-08-23 |
| SE8705074D0 (sv) | 1987-12-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAL | Patent in force |
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