RU95106503A - Способ увеличения концентрации кислоты-мишени, способы определения присутствия выбранного нуклеотида, наборы - Google Patents

Способ увеличения концентрации кислоты-мишени, способы определения присутствия выбранного нуклеотида, наборы

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Publication number
RU95106503A
RU95106503A RU95106503/13A RU95106503A RU95106503A RU 95106503 A RU95106503 A RU 95106503A RU 95106503/13 A RU95106503/13 A RU 95106503/13A RU 95106503 A RU95106503 A RU 95106503A RU 95106503 A RU95106503 A RU 95106503A
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RU
Russia
Prior art keywords
oligonucleotide
methods
determination
sets
acid
Prior art date
Application number
RU95106503/13A
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English (en)
Inventor
В.Адамс Крэйг
Us]
В.Дэниелс Дэвид
Original Assignee
Бекман Инструментс Инк. (US)
Бекман Инструментс Инк.
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26748925&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RU95106503(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Бекман Инструментс Инк. (US), Бекман Инструментс Инк. filed Critical Бекман Инструментс Инк. (US)
Publication of RU95106503A publication Critical patent/RU95106503A/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Описаны способы и реагенты для детектирования и экспоненциальной амплификации молекул-мишеней нуклеиновых кислот. Способ, в основном, использует праймерный олигонуклеотид, который гибридизуется вместе с блокаторным олигонуклеотидом с цепью молекулы-мишени, и End - Run олигонуклеотид, который может гибридизоваться с блокаторным олигонуклеотидом.

Claims (1)

  1. Описаны способы и реагенты для детектирования и экспоненциальной амплификации молекул-мишеней нуклеиновых кислот. Способ, в основном, использует праймерный олигонуклеотид, который гибридизуется вместе с блокаторным олигонуклеотидом с цепью молекулы-мишени, и End - Run олигонуклеотид, который может гибридизоваться с блокаторным олигонуклеотидом.
RU95106503/13A 1992-08-04 1993-08-04 Способ увеличения концентрации кислоты-мишени, способы определения присутствия выбранного нуклеотида, наборы RU95106503A (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US92505992A 1992-08-04 1992-08-04
US07/925059 1992-08-04
US08/068,393 US6180338B1 (en) 1992-08-04 1993-05-27 Method, reagent and kit for the detection and amplification of nucleic acid sequences
US08/068393 1993-05-27

Publications (1)

Publication Number Publication Date
RU95106503A true RU95106503A (ru) 1997-05-10

Family

ID=26748925

Family Applications (1)

Application Number Title Priority Date Filing Date
RU95106503/13A RU95106503A (ru) 1992-08-04 1993-08-04 Способ увеличения концентрации кислоты-мишени, способы определения присутствия выбранного нуклеотида, наборы

Country Status (11)

Country Link
US (1) US6180338B1 (ru)
EP (1) EP0658212B1 (ru)
JP (1) JPH07509371A (ru)
AT (1) ATE251674T1 (ru)
AU (1) AU676197B2 (ru)
CA (1) CA2141537A1 (ru)
DE (1) DE69333242T2 (ru)
FI (1) FI950487A (ru)
PL (1) PL307341A1 (ru)
RU (1) RU95106503A (ru)
WO (1) WO1994003630A2 (ru)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08508636A (ja) * 1993-01-27 1996-09-17 オンコール インコーポレーテッド 核酸配列の増幅
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US6096880A (en) * 1993-04-15 2000-08-01 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US7135312B2 (en) 1993-04-15 2006-11-14 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
AU1911395A (en) * 1994-02-04 1995-08-21 Beckman Instruments, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
AU706033B2 (en) * 1994-05-23 1999-06-10 Biotronics Corporation Method for detecting a target nucleic acid
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
WO1996040992A2 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
DE69636080T2 (de) 1995-12-05 2006-11-30 Erland Jorn Koch Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
GB9609213D0 (en) * 1996-05-02 1996-07-03 Tepnel Medical Ltd Amplification of nucleic acids
AU730633B2 (en) * 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
GB9626074D0 (en) 1996-12-16 1997-02-05 Cytocell Ltd Nucleic acids amplification assay
FR2779154B1 (fr) * 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
US6911307B1 (en) 1998-12-08 2005-06-28 Proteus S.A. Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro
FR2786789B1 (fr) * 1998-12-08 2001-12-28 Proteus Methode de detection in vitro d'une sequence d'acide nucleique cible dans un echantillon d'acide nucleique
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6368834B1 (en) * 1999-04-06 2002-04-09 Genome Technologies, Llc PCR genome walking with synthetic primer
JP2001249996A (ja) * 1999-12-28 2001-09-14 Sony Corp ホームドクターシステム、血液収納用カプセルおよび注射装置
US20040146918A1 (en) * 2000-02-18 2004-07-29 Weiner Michael L. Hybrid nucleic acid assembly
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6942972B2 (en) 2001-10-24 2005-09-13 Beckman Coulter, Inc. Efficient synthesis of protein-oligonucleotide conjugates
ES2524048T3 (es) 2003-03-31 2014-12-03 F. Hoffmann-La Roche Ag Composiciones y métodos para la detección de ciertos flavivirus, incluidos los miembros del serogrupo del virus de la encefalitis japonesa
CA2538222A1 (en) 2003-09-12 2005-03-24 Stephen Bryant Liggett Methods for risk assessment, survival prediction and treatment of heart failure and other conditions based on adrenergic receptor polymorphisms
JP4933435B2 (ja) 2004-09-14 2012-05-16 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・コロラド，ア・ボディー・コーポレイト 遺伝子ターゲティングに基づくブシンドロール処置方法
WO2006081426A2 (en) * 2005-01-28 2006-08-03 Applera Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template
WO2006108423A2 (en) 2005-04-12 2006-10-19 In Situ Rcp A/S Under Founding Methods for production of oligonucleotides
WO2006108422A2 (en) 2005-04-12 2006-10-19 In Situ Rcp A/S Under Founding Methods for production of oligonucleotides
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
GB2466442A (en) * 2008-12-18 2010-06-23 Dublin Inst Of Technology A system to analyze a sample on a slide using Raman spectroscopy on an identified area of interest
CN103635593B (zh) 2011-05-04 2018-03-20 生物概念股份有限公司 用于检测核酸序列变体的方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
IL86164A0 (en) 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
US5093245A (en) 1988-01-26 1992-03-03 Applied Biosystems Labeling by simultaneous ligation and restriction
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
DE68927373T2 (de) 1988-06-24 1997-03-20 Amgen Inc., Thousand Oaks, Calif. Verfahren und mittel zum nachweis von nukleinsäuresequenzen
WO1990001069A1 (en) * 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
GB2225112A (en) 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
AU627809B2 (en) 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles
US4994370A (en) 1989-01-03 1991-02-19 The United States Of America As Represented By The Department Of Health And Human Services DNA amplification technique
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
ATE137269T1 (de) 1990-01-26 1996-05-15 Abbott Lab Verbessertes verfahren zur amplifikation von nuklein säurezielsequenz, einsetzbar für die polymerase und ligasekettenreaktion
CA2039517C (en) 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
HU218095B (hu) 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
ATE375392T1 (de) 1990-05-03 2007-10-15 Cornell Res Foundation Inc Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase
CA2049879A1 (en) 1990-08-30 1992-03-01 Keith C. Backman Controlled initial target-dependent production of templates for ligase chain reaction
CA2052413C (en) 1990-09-28 2004-07-13 Jeffrey L. Joseph Nucleotides sequences useful as type-specific probes, pcr primers and lcr probes for the amplifications and detection of human papilloma virus, and related kits and methods
AU1911395A (en) * 1994-02-04 1995-08-21 Beckman Instruments, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences

Also Published As

Publication number Publication date
WO1994003630A2 (en) 1994-02-17
PL307341A1 (en) 1995-05-15
CA2141537A1 (en) 1994-02-17
WO1994003630A3 (en) 1994-03-31
US6180338B1 (en) 2001-01-30
FI950487A (fi) 1995-03-10
ATE251674T1 (de) 2003-10-15
EP0658212A1 (en) 1995-06-21
DE69333242T2 (de) 2004-08-26
AU676197B2 (en) 1997-03-06
AU4802793A (en) 1994-03-03
FI950487A0 (fi) 1995-02-03
JPH07509371A (ja) 1995-10-19
EP0658212B1 (en) 2003-10-08
DE69333242D1 (de) 2003-11-13

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