RU2739252C2 - Method for initiation of tumor cell death by chlorine-e6, ascorbic acid and hf- and shf-energy - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention is intended for induction of tumor cell death in living biological objects highly electroconductive solution of sodium salt of Chlorine-e₆ 2–3 mg/kg of body weight and ascorbic acid 200–300 mg of electromagnetic HF- and SHF-energy of photo-wave radiation, known as HF- and SHF-hyperthermia. Described is a method for initiation of tumor cell death by HF- and SHF energy, intended for local treatment of oncological patients having tumor tissues, for their hyperthermia in all organs of human body, characterized by the fact that before treatment, the person is transferred to a protein diet for 3 days for maximum saturation of oncological cells with an electron-ion solution of the chlorine-e₆ sodium salt intravenously in megadose of 3.1 mg/kg of human body weight and orally in ascorbic acid megadose 500 mg of ascorbic acid is taken orally, for their maximum accumulation in tumor tissues, respectively, 3–4 times higher than in healthy tissues after 72 hours after administration of sodium chloride Chlorine-e₆ and ascorbic acid 4 hours before treatment, selective hyperthermia of tumor tissues SHF-energy is performed in accordance with depth of their location and penetration depth of electromagnetic wave into human body 6.1 centimeters, at resolved frequency f=2450 MHz, with total heating rate of tumor tissues at the allowed frequency of 0.010 °C/s, for 216 seconds to heating temperature of tumor tissues 58 °C, when heating healthy tissues no more than 40 °C.

EFFECT: described method leads to thermal death of tumor tissues due to high dielectric contrast of tumor and increase in difference of its conductivity (dielectric losses) relative to surrounding healthy biological tissues, reaching multiple values for various tumors; this allows for fluorescence diagnostics to specify boundaries of tumors and to conduct simultaneous hyperthermia of tumor cells by electromagnetic HF- and SHF energy of photo-wave radiation and to detect, and destruct, therefore, even undetectable tumor formations located in deep layers of biological object.

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Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects of the sodium salt "Chlorin-e ₆ " contained in the medical preparation "Chlorin-e ₆ " and ascorbic acid (AA) and the energy of wave HF and microwave radiation, known as HF and UHF hyperthermia.

Known methods for initiating the death of tumor cells by HF and UHF hyperthermia. Hyperthermia is referred to in medicine as a significant increase in the temperature of the human body over 40 ° C. Hyperthermia for cancer treatment has been used as early as half a century ago. The German physician von Ardenne opened a "thermal" clinic in a water bath for hopelessly oncological patients, whom he heated to 42 ° C. After such a procedure, no more than 17% of people survived, but they were completely cured. The rest died, unable to withstand such high temperatures. This technology is still used in the United States, where the human body is heated to 42.5 ° C, with its subsequent return to life. This treatment technology can be effectively used for selective heating of oncological tissues with HF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

The method of initiating the death of tumor cells by electromagnetic energy of wave radiation consists in the complex simultaneous action of sodium salts of Chlorin-e ₆ and ascorbic acid (AA), which include concentrate for preparing a solution for infusion of the drug “Chlorin-e ₆ ”. The preparation "Chlorin-e ₆ ", and AK dissolves very well in aqueous solutions. The accumulation of the sodium salt Chlorin-e ₆ in the tumor occurs within 48-72 hours after its intravenous administration. Then the level, after 72 hours, of the sodium salt Chlorin-e ₆ in the tumor gradually decreases, reaching the initial values 14 days after the administration of the drug Chlorin-e ₆ intravenously (drip) with a 30-minute infusion in a semi-darkened room in a single dose of 2-3 mg / kg of body weight with preliminary dilution in 40 ml of sterile saline, the drug is stored in a dark place, 24 hours before diagnostics and hyperthermia of tumor tissues with HF and microwave energy of wave radiation. The maximum content of sodium salt Chlorin-e ₆ and AA in tumor tissues occurs precisely during this period of time, and it is 3-4 times higher than in healthy tissues, due to the selective absorption of this salt by tumor tissues. The drug is excreted and redistributed from normal tissues and the human circulatory system into the tumor in a day, 94% after intravenous administration. With simultaneous, during this period of HF and UHF, selective hyperthermia of tumor cells for 235 seconds by wave radiation at the permitted frequency of oscillations of the electromagnetic field f = 13.56 MHz; f = 40.68 MHz; f = 433 92 MHz; f = 915 MHz and f = 2400 MHz, with a heating rate of 0.044 ° C / sec to a final temperature for heating tumor cells of 47 ° C. The concentration of the drug "Chlorin-e ₆ " in the tumor reaches its maximum values during the first 3-5 hours after its intravenous administration and remains during the day, in other organs and tissues the concentration of the drug, during this period of time, sharply decreases, which causes maximum damage tumors during HF and UHF electromagnetic hyperthermia during this period of time. After 5 hours, after its intravenous administration, there is a faster excretion of "Chlorin-e ₆ " from healthy tissues in comparison with tumor ones. This results in a high fluorescent contrast of the tumor and an increase in its conductivity (dielectric properties) relative to the surrounding healthy biological tissues, reaching a difference of 3-4 times for different tumors. This allows, during fluorescence diagnostics, to clarify the boundaries of tumors and simultaneous hyperthermia of tumor cells with the energy of wave radiation and to identify and destroy, thus, even undetectable tumor formations located in the deep layers of a biological object with the energy of wave radiation.

The famous scientist Launus Pauling conducted scientific data on the positive effects of ascorbic acid on slowing down the cancer process. Some experts explained this by the fact that AK was taken by sick people in large doses in the form of tablets and did not always give a positive effect due to the fact that the concentration of AK in the tumor itself was low. It has been suggested that the tumor suppression mechanism is associated not with AA, but with its oxidized form. This has been confirmed by scientific research. In experiments where Dehydro-AK was taken, the results were constantly renewed. These results were published in the 1980s and early 1990s. Their most complete description was given in 1991 in the journal of the American Association for Clinical Nutrition. Good results were obtained for leukemia in mice. The skepticism of the scientific world, however, did not allow paying serious attention to these data. Lonely physicians continued research on the treatment of cancer with vitamin C, without delving into especially the theoretical basis of this process. A Canadian researcher John Teway tried to uncover the mechanisms of action of AK on tumor cells. His final work was published in Cancer letters in 2008. Currently, physicians use AK as an additional agent that reduces the side effects of chemotherapy.

Known complex solutions of sodium salt "Chlorin-e ₆ " and ascorbic acid. This complex of sodium salt "Chlorin-e ₆ " and ascorbic acid on the cell membrane is taken by the oncocells for glucose.

During high-frequency and microwave heating of tumor tissues, the sodium salt "Chlorin-e ₆ " is oxidized by hydrogen peroxide of a derivative of dehydroascorbic acid (D-AA) in an alkaline medium, the reaction is catalyzed by iron heme, and causes chemiluminescence with active release of singlet oxygen. If an oxidizing agent - hydrogen peroxide is added to an alkaline solution of cancer cells, then a glow occurs. In the presence of catalysts, this glow intensifies and becomes brighter. The role of catalysts for the solution of sodium salt of the drug "Chlorin-e ₆ " is carried out by blood iron hemin and various sodium compounds. These chemical activators of chemiluminescence enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. The observation with this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is sodium salt "Chlorin-e ₆ " in the presence of oxygen radicals. Under the action of the AA oxidant - hydrogen peroxide radicals, there is a formation, which reacts with a superoxide radical, forming an internal peroxide (dioxide), which leads to the formation of an excited molecule of sodium salt of the drug "Chlorin-e ₆ ". The transition of this molecule to the initial ground state is accompanied by emission of a quantum of light. Hydrogen peroxide is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, it is a relatively harmless compound, but in the presence of metal ions of variable valence iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide Н ₂ О ₂ , a destructive hydroxyl radical is formed JOH, which causes mutations and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl group of enzymes activates molecules, and actively enters into a chemical reaction with it in biological tumor tissues and tissue macrophages, in the fight against foreign cells, they release reactive oxygen species contained in superoxide radicals, hydrogen peroxide Н ₂ О ₂ , and hydroxyl radical JOH in In this case, a weak chemiluminescence is observed, which is increased many times in the presence of the sodium salt of the drug "Chlorin-e ₆ ". These effects are also multiplied by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by mitochondria of cells, which leads to a bright glow.

When placed in a variable electromagnetic field of high intensity and frequency of various biological bodies, they begin to emit a characteristic radiance of varying intensity and colors, which can be used to judge the properties of the object under study. The method of "high-frequency photography" (the Kirlian effect, Kirlianography in honor of the inventor V.Kh. Kirlian) is now widely known in Russia and abroad as a method of experimental research of electromagnetic fields and bioenergetic interactions. But the research of the glow of biological objects in a variable electromagnetic field of high frequency is of the greatest scientific and practical interest.

In accordance with modern concepts, aqueous solutions of alkalis and acids in the human body is considered as an associated liquid [7], consisting of individual associated elements - neutral clusters and cluster ions of the general formula (Н ₂ О) _n , [(H ₂ O) _n ] ⁺ , [(H ₂ O) _n ] \ [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ] etc. where the number of hydrogen-bonded water molecules can reach n times, according to some authors, hundreds and even thousands of units [8]. A change in the position of one structural element (water molecule) under the influence of any external factor or a change in the orientation of the surrounding neighboring water molecules provides a high sensitivity of the entire information system of water to various external influences (electromagnetic, thermal, sound fields, bioinfluence, etc.).

In addition, in water clusters, due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, the proton (H ⁺ ) can migrate according to the relay mechanism, leading to proton delocalization within the cluster. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-information, etc. [10]. In addition, aqueous solutions of various chemical elements are a source of superweak and weak alternating electromagnetic radiation. In this case, induction of the corresponding electromagnetic field and resonant effects of combination (superposition) of electromagnetic fields that can change the structural and informational characteristics of biological objects, 80-90% consisting of water with various chemical impurities, can occur.

Under the influence of a high-frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, excitation, polarization and ionization of N ₂ , H ₂ , O ₂ and CO ₂ molecules occur. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of the object, can color the glow corona in different colors. The shape of the glow crown, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body, granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells, release reactive oxygen species contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and the hydroxyl radical JOH in this case, weak chemiluminescence is observed, which increases many times in presence of D-AK and AK at HF and microwave irradiation. These effects are also greatly enhanced by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the mitochondria of cells.

This effect of electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nikola Tesla, when exposed to pulsed high-frequency energy of vessels with liquids capable of emitting light and fluorescent lamps, which, without being connected to electrical wires, shone with bright light in the hands of Nikola Tesla, with which he also juggled, which caused genuine delight among the audience, while at that time inexplicable by nature, a phenomenon that only Nikola Tesla knew.

In biology, these effects are called collective luminescence stimuli that change the state of phagocytes in blood and tissues and their ability to increase the release of reactive oxygen species and, accordingly, the protective functions of cells.

In cancer cells, aerobic respiration is absent in mitochondria and is replaced by glycolysis. Upon entering the oncocell, AA inhibits glycolysis, but is unable to transfer it to the path of normal aerobic activity. Perhaps this is due to the competitive presence of glucose.

To completely turn off glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or so that AA and the sodium salt of Chlorin-e ₆ prevail in the substrate over glucose and under the influence of the electromagnetic field of HF and microwave, causing them to heat up, with a large release of hydrogen peroxides and the formation of luminol, the main activators death of tumor cells. In healthy cells, AA and ALA in small amounts in the cytosol, it exhibits protective antioxidant properties. In oncological cells, with their excess, oxidation processes are stimulated, with the formation of hydrogen peroxide and lipoperoxides, which have a stable toxic effect on oncocells.

It can be argued that the effect would be higher if treatment with the sodium salt of Chlorin-e ₆ and AK was based on the background of a complete overlap of the intake of carbohydrates - glucose, as competitors of the sodium salt of Chlorin-e ₆ in cancer cells. In cancer cells, aerobic respiration is absent in mitochondria and is replaced by anoxic glycolysis. The sodium salt of Chlorin-e ₆ together with AA, when it enters the oncocell, inhibits glycolysis, but is unable to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely turn off glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or for AA and the sodium salt of Chlorin-e ₆ to prevail in the substrate over glucose. In healthy cells, in small amounts in the cytosol, it exhibits protective antioxidant properties. In oncological cells, with an overabundance of AA and ALA, and the additional simultaneous effect of HF and SHF electromagnetic hyperthermia on onco cells, oxidation processes are stimulated, which, when they are overabundant, have a toxic effect on onco cells. To do this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract are converted into glucose, which is extremely necessary for the nutrition of cancer cells. With this introduction of onco cells into artificial glucose "starvation", then a person needs to inject high single doses of 2-3 mg / kg of body weight of sodium salt Chlorin-e ₆ and 500 mg of AA. The required amount of the drug based on the maximum permissible one-time megadoses of sodium salt Chlorin-e ₆ , not exceeding 2-3 mg / kg and 500 mg of AA. Under the action of enzymes, in the human body, sodium salts Chlorin-e ₆ and AA accumulate and are determined by the intracellular concentration (the level of accumulation of the sensitizer) by its localization in the cell and photochemical activity (quantum yield of the generation of singlet oxygen or free radicals), providing a fluorescent tumor contrast and an increase in its conductivity relative to the surrounding healthy biological tissues. When the sodium salt Chlorin-e ₆ and AA enter the tumor blood vessels, which have a large branched network with thin peripheral vessels and a low blood flow rate in them, the blood flow in these vessels of tumor tissues decreases even more when they are heated, which leads to blood coagulation in the vessels of tumor tissues, not allowing them to cool down, due to the absence of a closed circulatory system. This is a direct cytotoxic effect on tumor cells, disrupting their blood supply, due to damage to the endothelium of the blood vessels of the tumor tissue, due to the hyperthermic effect and cytokine reactions, while there is an activation of macrophoges, leukocytes and lymphocytes, leading to tumor necrosis. In the main human organs, rich in blood vessels, closed in the main circulatory system, there is a cooling of borderline healthy tissues exposed to HF and UHF hyperthermia. A "hungry" tumor is maximally saturated with the sodium salt of Chlorin-e ₆ , and AA, as a glycolysis transport, is 3-4 times higher than in normal healthy tissues, and accumulates in a sufficiently large amount on membranes and in interstitial fluid.

Dehydroascorbic and sodium salts of Chlorin-e ₆ , are actively imported into endoplasmic reticules (EPR) (Endoplasmic reticulum, consisting of membranes and giving direction and active transport of substrates against gradients) of cells with the help of glucose transporters. It should be noted that energy processes in cancer cells are transferred from mitochondria to the endoplasmic reticulum. It is here in the EPR that D-AA and sodium salt Chlorin-e ₆ accumulate and the environment of the oncocell in this place differs significantly from ordinary cells, they are simply re-reduced here and here D-AA is obviously forced to be reduced to AA, and the sodium salt of Chlorine is e ₆ to hymns. From this moment on, the destructive effect of AA and the sodium salt of Chlorin-e ₆ on the oncocell begins. A "hungry" cancer cell at this time can repeatedly accumulate D-AA and sodium salt of hematoporferin, because perceives them on its membrane transporters for glucose. Since there are many times more glucose-consuming receptors in the oncolet than in healthy people, although the transport systems for the delivery of glucose and D-AA and the sodium salt of Chlorin-e ₆ into cells are common, this is the "Trojan horse" for oncocells. Thus, it is very easy to deceive the oncocells and inject D-AA and sodium salt Chlorin-e _{6 into them} , with the solution of the problem of supplying megadoses of D-AA and sodium salt Chlorin-e _6, and then the phenomenon of death of onco cells will be greatly enhanced.

A "hungry" tumor in the absence of glycolysis is maximally saturated with the sodium salt of Chlorin-e ₆ and ascorbic acid, 3-5 times higher than in normal healthy tissues, stimulates the formation of macrophages and T-lymphocytes under the action of the enzyme ferrachelatase, in a sufficiently large amount for membranes and interstitial fluid. It is this chemical compound that is formed during the interaction of the sodium salt of Chlorin-e ₆ and ascorbic acid in the internal environment of the body. Under the action of the oxidizing agent of lipoperoxide radicals and hydrogen peroxide, significantly enhanced by the temperature action and the additional action of the HF and UHF electromagnetic fields, superoxide radicals are formed that accelerate and form internal peroxide (dioxide) and hydrogen peroxide H ₂ O ₂ during HF and UHF hyperthermal decomposition, AK of vitamin C. In this case, there is a multiple increase in the formation of excited oxygen molecules. The transition of sodium salt dioxide molecules from the excited to the ground state is accompanied by the emission of light quanta and a strong glow. As a result of these chemical reactions associated with the high release of reactive forms of hydrogen and oxygen and organic free radicals, cancer cells are burned out.

The method of "selective starvation" of superficial and deeply located onco cells in the human body, by subsequent administration or administration of various sensitizers, for selective maximum saturation of tumor cells with highly electrically conductive electron-ion solutions of electrophotosensitizers with a maximum separation of the electrophysical properties of tumor and healthy tissues with subsequent selective action on them electromagnetic fields of high frequency in combination with other methods - this is the most relevant scientific and practical direction in the fight against oncological diseases

The study of biophysical and biochemical mechanisms of the complex effect of HF and microwave energy on onco cells saturated with AA and sodium salts of Chlorin-e ₆ suggest three concepts of death of onco cells, one assumes the significance of D-AA and AA, and the other, sodium salt Chlorin-e ₆ , the third significance of HF and microwave hyperthermia, which, with the simultaneous processing of high frequency and microwave energy with the energy of tumor tissues saturated with the above solutions, in both cases leads to a clear 100% death of oncocells.

In healthy cells, D-AA and sodium salt Chlorin-e ₆ , entering the EPR. will not recover to AK and ALK, because pH and ORP (oxidation-reduction potential) are not suitable for this, and D-AK and ALA will be practically harmless for them and will be transformed on the glucose conveyor. In oncocells, the environment is different, reconstituted into ascorbic acid and sodium salt of Chlorin-e ₆ , which try to burn and destroy everything in the oncocell to the maximum, due to lipid peroxidation (LPO). In this case, significant destruction occurs with the formation of toxic lipoperoxides that damage cell membranes, various organelles, mutation of nucleic acids, enzyme inoculation, destruction of nutrients and cell death. In this case, cell death follows the path of necrosis rather than apoptosis.

Ascorbic acid and sodium salt Chlorin-e ₆ , in oncocells is converted into dehydroascorbic acid, and sodium salt Chlorin-e ₆ , under the action of temperature, with the formation of hydrogen peroxide and other lipoperoxides. The more dehydroascorbic acid and sodium salt Chlorin-e ₆ in the oncocell, the more lipoperoxide and hydrogen peroxide are formed in it, in comparison with healthy cells. An excess of hydrogen peroxide and lipid peroxide triggers the death of cancer cells. The process of death of cancer cells is initiated by HF and UHF fields by rapidly heating cancer cells to 47 ° C, saturated with D-AA, and the sodium salt of Chlorin-e _6, their rapid oxidative decomposition under the influence of temperature with a large release of hydrogen peroxide and lipoperoxides, which is detrimental to cancer cells ...

A number of researchers argue that the minimolar concentration of vitamin C, which is a prooxidant (lyco-oxidizing compounds that nitralize free radicals), in the blood and tissues kill cancer cells without affecting healthy ones, due to the damage caused by local oxidative stress-the process of damage as a result of oxidation, cellular DNA and depletion of adenosine triphosphate (ATP), the cell's energy source. Hydrogen peroxide, along with other accompanying molecules of aggressive action, causes a malfunction of the functioning of a certain enzyme responsible for the "nutrition" of cells of malignant tumors. AA and sodium salt Chlorin-e ₆ , can accumulate in the cytosol of cells, disrupt the endothelium of blood vessels of tumors and cytokine reactions that stimulate TNF-a, activate microphages, leukocytes and lymphocytes, actively damage tumor cells.

The main task for researchers is to enhance as much as possible the effect of maximum selective absorption of D-AA, AA and sodium salt Chlorin-e ₆ by cancer cells with simultaneous electromagnetic hyperthermia of tumor cells of HF and SHF energy in order to increase the effectiveness of treatment up to 100% ... It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. Numerous studies carried out by us at the Krasnoyarsk State Agrarian University and VIZR in St. Petersburg have confirmed the 100% efficiency of disinfection of vegetable seeds and living biological objects saturated with highly conductive electron-ionic solutions of HF and microwave energy against viral infections of a similar origin with oncocells.

A.S. No. 563938 USSR. Method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bul. No. 25. Certificate of the USSR No. 950214. Method of pre-sowing seed treatment / Tsuglenok N.V. - Registered in the registry on 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with HF and UHF energy: guidelines / N.V. Zuglenok. - M .: Agropromizdat, 1989. Guidelines for the use of high-frequency and microwave energy in the preparation of seeds for sowing / N.V. Zuglenok. - M .: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfection of tomato seeds against viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute of Plant Protection (VIZR). - 1989. - T. 71. - S. 49-54. Method for disinfecting egg powder. Patent number: 1734632. Published: 23.05.1992 Authors: N. V. Tsuglenok Kolmakov Yu.V. IPC: A23v 5/02. Method of preparing a medium for diluting semen from the manufacturer. Patent number: 1769422. Published: 27.06.1995. Authors: Tsuglenok, Ostashko, Shakhmatov, Silantyeva, Kontsedal.

It has been proven that under the influence of carcinogens, oncoviruses are incorporated into a healthy cell and eventually dissolve in it, turning it into an oncocell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are mostly powerless, they simply do not exist. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the shallow depth of penetration of the electromagnetic wave of laser emitters does not allow burning deep-seated malignant tumors.

It is necessary to note another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein shell filled with a mixture of nucleic acids and, similarly, tumor cells filled with solutions of intercellular fluid determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. With an increase in temperature during HF and microwave heating in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity and dielectric losses, which further enhances their selective heating and apoptosis of tumor tissues.

This curing effect is explained by the fact that at this time from 3 to 5 hours in normal cells of living biological objects the ascorbic and sodium salt of Chlorin-e ₆ is rapidly converted into dehydroascorbic acid and bivalent iron heme, under the action of the ferrochelatase enzyme, while maintaining a high contrast the content of dehydroascorbic acid and sodium salt Chlorin-e ₆ , and their transformation into ascorbic acid in the tumor, which significantly increases its electrical conductivity with a significant change in the dielectric properties of tumors, relative to the surrounding healthy biological tissues, reaching this difference many times.

Tumor cells accumulate, in contrast to normal cells, a significant amount of homocysteine theolactone (HTL). Before homocysteine is inserted into a protein, it becomes a biological marriage, in the form (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone is practically not formed from it, but transformation into a cancer cell requires a significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein that synthesizes the machine of the cancer cell works at full capacity, so it is more often mistaken. Teway discovered that interaction with dehydroascorbic acid, this substance forms the highly toxic 3-mercaptopropionic aldehyde (MPA). When Dehydroascorbic acid and the sodium salt of Chlorin-e ₆ enter a cancer cell saturated with HTL, MPA is formed, which kills the cancer cells. Destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer from it much under the influence of the sodium salt Chlorin-e ₆ . In this case, it can be argued that in the treatment of cancer with the dehydroascorbic and sodium salt of Chlorin-e ₆ , obtained as a result of the oxidation of AA and the sodium salt of Chlorin-e ₆ , both in the blood and in various human organs, a therapeutic effect is observed.

The biophysical meaning of this method lies in the selective maximum saturation and accumulation of highly electrically conductive electron-ion solutions of electrophotosensitizers in tumor cells and in the maximum separation of the electrophysical properties of tumor and healthy tissues of dehydroascorbic and sodium salt of Chlorin-e ₆ , and a significant increase in the difference between healthy cells of tumor and tumor cells. in the intercellular environment and on the walls of the reticulum. The reticulum is an electrical circuit, where negative charges are evidently accumulated on one side of the membrane, and positive ones on the opposite side, therefore the reticulum is an electric transporter of glucose and other nutrients of cancer and healthy cells. Consequently, the reticulum is an electrical network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of mitochondria and the energetic operator structures on the outside of the cell - on the cilia. These proteins under certain situations in the environment of the cell, being discharged, can give an active signal to the reticulum and mitochondria. This changes the balance that exists on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are triggered. One side of the reticulum membrane is connected to one type of entrance to the mitochondria, and the opposite side to the exit from it. Thus, a single electrical circuit is created for double active control of mitochondrial energy. The intensity of the electric field on the reticulum keeps mitochondria under control. In this case, the mitochondria draw in the charges accumulated on one side of the reticulum membrane, and remove the opposite charges to the other side of the reticulum membrane. The charges are thus not mixed and separated. This is important for ion exchange to take place in cells. Externally, the reticulum is similar to the capacitor plates, the more layers of plates, the greater its electrical capacity. Between the gaskets there is a semiconductor saturated with dehydroascorbic, ascorbic acids and the sodium salt of Chlorin-e ₆ . This capacitor, i.e. a powerful dense network of tumor sheaths-membranes is very clearly visible through a microscope. The number of membranes in tumor cells is much higher than in healthy ones. Accordingly, the density of tumor tissues and the capacity of the biological electrical capacitor are significantly higher than those of healthy tissues. When charging, negatively charged particles-electrons will be collected on one plate of such a capacitor, and ions, positively charged particles, will be collected on the other. Such a charged capacitor can turn into a current source if disconnected. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which is dumped on the mitochondria, controlling their activity. The mitochondria, in turn, are tuned so that they never allow the charges on the reticulums to drop below a critical level. In cancer cells, the charges inside the mitochondria are sharply reduced and the entire regulation system is disrupted. This is the main control rod for the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not primary. As a result of the electrochemical energetics of the cell in the reticulum there is a cycle of substances, where the mitochondria are the pump.

With a lack of this circulation between the reticules and mitochondria, due to electroosmosis, substances are sucked from the outside through the outer membrane and the gateways and sodium pump are opened on it. The environment on the membranes of the reticulum and an alkaline liquid substrate in tumor cells is re-reduced due to the excess of negative charges. This is what determines the chemical equilibrium in pH of conjugated buffer chemical electroparic substances when the buffer system is discharged or restored. These processes are regulated by charges on the lining of the reticulum and mitochondria. Chemical processes, in this case, are simply executors, intermediaries. The outer side of the mitochondria provides voltage to the dependent anion channel. This voltage maintenance mechanism is called VDAC, which sets the conditions for the work of the reticulum. It is here on the outside of the membrane that the Hexokinase II enzyme is located, which ensures the utilization of glucose or its substitutes. Separation, disconnection of the work of the outer mitochondrial membrane (VDAC) and Hexokinase II ensures the induction of apoptosis of tumor cells.

The mitochondrion works by pulling from the reticulum into itself as an electromagnetic pump, which requires power under high voltage. Without this effect, the highest tension of pulling in nutrients into the cell will not be possible. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Cancer cells, unlike normal cells, do not have cilia. This most damaging energy level is absent in cancer cells. The only correct way in the fight against cancer cells is to find a weak point in the energy of cancer cells and thereby destroy them. Mitochondria set the state of charge for ion pumps on the outer membrane of the cell and for starter structures that hold charges on the reticulum. These sensory structures can be most rapidly damaged and burned out, since mitochondria are the most efficient electrochemical furnaces. In the case of disconnection of mitochondria, the voltage gradient of the cell decreases sharply and the processes take place in onco cells over a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. The oncocell does not take quality, since everything is concentrated on a small area of mitochondria, but with a larger number of them, much larger than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but the consumption of glucose will be much greater than in healthy tissues.

Membranes - reticulums and cell nuclei are the same, and the reticulum as a capacitor is contoured to the nucleus, with only one side of it - an electron and dumps electrons into the nucleus. Thus, the charge in the reticulum also provides charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a super-powerful electrostatic charge inside the nucleus.

The main task for researchers remains to enhance as much as possible the effect of maximum selective absorption of ascorbic acid and sodium salt of Chlorin-e ₆ by cancer cells and increase the effectiveness of treatment by increasing the electrical conductivity of mitochondria and reticulums of cancer cells with simultaneous selective HF and SHF. hyperthermia of cancer cells. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. The electrical conductivity of cancer cells is due to the presence in them of mobile charged electrons on the reticulums and in the cell nucleus and ions in the mitochondria of the cell. The amount of electrical conductivity depends on the amount of electrical charges and their mobility. The electrical conductivity of living tissues is determined by the concentration of ions and their mobility, which is different in different tissues, and therefore biological objects have the properties of conductors, semiconductors and dielectrics.

The intercellular fluid, saturated ascorbic acid and sodium salt Chlorin-e ₆ contains the maximum content of ions and the specific conductivity of tumor tissues is high and is more than 1 S ⋅ m ^-1 . Large protein molecules have a lower electrical conductivity, up to 0.003 S ⋅ m ^-1 . Intracellular membranes have a conductivity below (1-3⋅10 ^-5 ) S ⋅ m ^-1 . The highest value of conductivity in the human body have liquid medium (blood, lymph, bile, urine, cerebrospinal fluid and tumor cells (See 0.6-2.0 ^⋅ m-1) and muscle tissue (see 0.2 ⋅ m ^{- 1).} The lowest conductivity has bone, fat and nerve tissue, especially tissue and fibrous connective tissue enamel (10 ^-3 -10 ^-6 See ⋅ m ^-1). significantly more complicated is electroconductivity cells and tissues at RF and microwave currents.In this case, biological objects have both conductivity and capacitance, which characterizes the dielectric constant.The frequency dependence of electrical parameters and the absorption of energy of the electromagnetic field are determined by the size and shape of cells, the value of their permeability, the ratio between the volume of cells and intercellular spaces, concentration free ions in cells and the content of free water in them.All these factors lead to a change in the electrical conductivity of biological objects ... A particularly significant factor for the metabolism of cancer cells is the content of glucose or its substitutes, in this case, ascorbic and sodium salts of Chlorin-e ₆ . If the human body has malignant tumors and metastases of stages 3 and 4, which actively and intensively assimilate glucose or its substitute - ascorbic acid and sodium salt of Chlorin-e ₆ , they are converted into ATP in cancer cells much lower than in healthy ones, as a result which, cancer cells are very hot and increase the temperature of the human body by 1-2 ° C. This physiological mechanism induces an increase in the temperature of tumor and surrounding normal tissues. The total temperature rise, at present, is recorded by a microwave radiometer with an accuracy of 0.3 ° C, when determining the temperature of deeply located tumor and healthy tissues.

This process was partially studied by us when exposed to a biological object with tumor tissues when exposed to magnetic fields, which were subjected to daily complex action of a constant magnetic field with an intensity of 25 μT and an alternating magnetic field with a frequency of 3.1 Hz and an intensity of 5 μT, an exposure of 60 minutes a day at a time, for 5 days. The proposed method of influencing constant and variable effects on ion exchange in mitochondria of cells and on negatively charged electrons on reticulums and cell nuclei made it possible to induce death of tumor cells using magnetotherapy, which by 40%, compared with control, freed biological objects from tumor cells ( Patent No. 2307681, authors: Tsuglenok N.V., Sergeeva E.Yu., Klimatskaya L.G. RU). Therefore, this direction of using magnetic and electromagnetic fields and their effect on the energy of tumor cells deserve special attention, confirmed by researchers from South Korea, who suggested using a powerful magnetic field to destroy tumor cells. In a powerful magnetic field, the tumor begins to kill itself.

A known method of destroying cancer cells with microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the value of the resonant frequency is absorbed by the tumors. After that, a similar effect is carried out on healthy tissues bordering with the tumor and the value of the resonance frequency of absorption of these healthy tissues is determined. Simultaneously with hyperthermia, the values of resonance frequencies of energy absorption by tumors and healthy tissues are monitored, and when the values of resonance frequencies approach, energy absorption by tumors and healthy tissues is judged on the effectiveness of treatment. This method improves the efficiency of tumor treatment by microwave hypothermia when heated to 43 ° C.

The main disadvantage of this method is the slight difference in heating of tumor and healthy tissues.

There is a known method for the destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6). The essence of the invention includes the introduction of ferromagnetic particles into the area of tumor localization, followed by induction local heating, in the temperature range from 42 ° C to 45 ° С, in the course of time, determined by the type of tumor, its size, localization and the type of ferromagnetic particles selected for induction heating, while heating is carried out only at the moments of a decrease in tissue blood filling, i.e. at the moments of exhalation and diastope of the patient's heart. The heating range is controlled by a microwave deep thermometer, and heating is carried out automatically, with the help of a computer, in the biocontrol mode, according to the algorithms of the mathematical model of fluctuations in the thermal conductivity and heat capacity of the tissue, the hysteresis of heating and heat removal.

The main disadvantages of this method are the low localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in different spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, Serdar Deger, Stefan Loening, Wolfgang Lanksch, Roland Felix. Journal of Magnetism and Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on thermolysis of magnetic nanoparticles injected into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions through cell membranes, as well as generate inductive alternating electric fields that affect the operation of neural networks in the human body, associated with heating not only magnetic particles, but all cells. located in the area of injection of magnetic particles, and a strong spatial inhomogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

The known method of close-focus X-ray therapy with a total focal area of 100-120 Gy and remote gamma therapy with radiation destruction of malignant cells with a total focal area of 30-40 Gy (see Sh.Kh. Gantsev. Oncology, M .: Medical Information Agency 2004, p. . 190-204; Stephen J., Withrow E., MacEwen G. Smal animal clinical oncology - 2001, p. 305-308).

However, this method, despite its prevalence, has the following disadvantages. In the treatment of certain types of malignant neoplasms, for example melanoma, using gamma remote therapy, even in combination with immunotherapy, experience shows that it leads to 75-90% of tumor recurrence, and after 2-6 months metastases occur.

The known method of neuron - capturing selective destruction of melanoma (see V. N. Mitin, N. G. Kozlovskaya, AM Arnopolskaya Neuron-capturing therapy of tumors of the oral cavity in dogs. All-Russian veterinary journal. 2006. No. 1, pp. 9-10) ...

The method includes intravenous injection of L-borphenylalanine into the blood, which selectively accumulates in a certain tumor - melanoma, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, there is a selective accumulation of L-borphenylalanine in melanoma cells. When a spatial zone comparable to a tumor containing L-borphenylalanine is irradiated by a beam of slow neurons obtained through a neuron guide from a nuclear reactor, melanoma cells are destroyed due to induced secondary local boron radiation.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced by using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to service.

3. Long-term irradiation of patients within an hour while monitoring the cardiovascular system.

4. Application of general anesthesia.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength coinciding with the absorption band of the photosensitizer (see Photodynamic therapy / Ed. TJ Dougherty / J. Clin. Laser Med Surg. 1996, Vol. 14, P219-348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of the accumulation of the photosensitizer in tumor cells as compared to healthy cells, which is associated with a high density of blood vessels in a tumor as compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, dye molecules pass into an excited electronic state, and upon collision with oxygen molecules dissolved in biological tissue, they transfer it from an unexcited to an excited electronic singlet state, with a typical lifetime of several microseconds. During this time, singlet oxygen molecules, having traveled a characteristic path commensurate with the size of cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, the destruction of cells occurs only during exposure to laser radiation in the spatial area of laser irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. The photosensitizers used in practice - phthalacionines, porphyrins, chlorins - have absorption bands of photosensitizers in the ultraviolet or visible region of the spectrum, and the lasers used cannot effectively penetrate to a depth of less than a few millimeters. In addition, the photodynamic method has a low contrast of the accumulation of photosensitizers in cancer cells.

The closest to the claimed method is the destruction of biological tissue, which consists in the introduction of ethanol into it using a hollow game, characterized in that 95% ethanol is introduced in an amount equal to half the volume of biological tissue to be destroyed, then 5 ml of 20-30% ethanol is introduced, after which is carried out by heating with a high-frequency current with the simultaneous introduction of 20-30% ethanol in an amount equal to the volume of biological tissue to be destroyed. The device contains a high-frequency current generator with two cylindrical electrodes located coaxially relative to each other, internal in the form of a hollow needle through which ethanol is injected into the tumor (Abstract No. 2006113533 of the RF patent application). The disadvantages of this method include: the unreasonableness of selective absorption of ethanol by cancer and healthy cells, the difficulty of inserting a coaxial electrode into heterogeneous tumors, to organize uniform heating of tumor tissues not equally located from the exposed end of the needle.

The objective of the present invention is the local selective destruction of malignant tumors deeply located in human biological tissues, preselectively maximally saturated for 3-5 hours with ascorbic acid and sodium salt Chlorin-e ₆ irradiated after 5 hours with maximum saturated wave electromagnetic energy with simultaneous selective HF and microwave - heating tumors to a temperature of 47 ° C at a heating rate of 0.044 ° C / sec, in order to increase the release of hydrogen peroxide in them, ascorbic acid is administered 3 hours before HF and microwave irradiation, for the complete destruction of tumors by photodynamic hyperthermia with minimal destruction of the surrounding healthy biological tissue cells, due to contact heat transfer from the tumor to the boundary layer of healthy tissues, heated at the same time to a temperature not higher than 40 ° C, after switching off the HF and microwave energy supply from the heated tumor tissues.

A method for initiating the death of tumor cells with high-frequency and microwave energy, including preliminary saturation of tumor cells with solutions of the sodium salt of Chlorin-e ₆ and ascorbic acid taken orally by a person, for its maximum accumulation, 3 to 4 times higher than in tumor tissues 3-5 hours after reception, characterized in that within 3 days before treatment, a person is transferred to a protein diet, in order to maximize the multiple selective accumulation in tumor cells of electron-ion solutions of the sodium salt of Chlorin-e ₆ and ascorbic acid, which increase the electrical conductivity of tumor tissues in comparison with healthy, and after 5 hours after taking the sodium salt of Chlorin-e ₆ and taking ascorbic acid, 3 hours before treatment, selective high-frequency and microwave electromagnetic hyperthermia and photoluminescence therapy of tumor tissues with the energy of wave radiation, with a heating rate of tumor tissues of 0.044 ° C / sec. , within 235 seconds to the temperature of tumor cells aney 47 ° С, when heating healthy tissues not higher than 40 ° С.

A one-time megadose of 3.1 mg / kg of sodium salt of Chlorin-e ₆ is injected intravenously and a one-time megadose of 500 mg of ascorbic acid is taken orally before HF and microwave treatment of tumor tissues and heating and hyperthermia of tumor tissues saturated with a solution of sodium salt of Chlorin-e ₆ and ascorbic acid HF and microwave acids with the energy of wave radiation, in accordance with the frequency, with the depth of penetration of the electromagnetic wave and with the depth of the location of tumor tissues, at the permitted frequencies f = 13.56 MHz-1100 cm, f = 27 MHz-545 cm, f = 40, 68 MHz - 370 cm, f = 433.92 MHz - 34.5 cm, f = 915 MHz - 16.5 cm and f = 2450 MHz - 6.1 cm.

According to studies carried out on HF and UHF hyperthermia of tumor tissues, at a temperature of 47 ° C, the border between the zone of necrosis and healthy tissue is several cells. The zone of destruction of tumor tissue includes a small zone of the periphery of normal healthy tissues, which excludes the movement of degenerating cells from metastasis through their secondary necrosis during contact heat transfer. The physical nature of microwave radiation is a physical field of moving electric charges, in electric and magnetic fields, which are a single electromagnetic field (EMF), characterized by the oscillation frequency f. The only difference is in the frequency with which electromagnetic oscillations of the corresponding wavelength occur. The biological effect of EMF on a living organism consists in the absorption of energy by biological tissues, characterized by biophysical parameters - dielectric constant and conductivity.

The tissues of the human body, due to their high water content, should be considered as dielectrics with losses. With general body irradiation, the EMF energy penetrates to a depth of 0.5 wavelengths. The intensity of exposure, exposure and dielectric losses and conductivity characterize the selective absorption of EMF by different tissues at the same density of EMF radiation.

where λ is the wavelength,

c is the speed of propagation of an electromagnetic wave,

f is the frequency of oscillations of the electromagnetic field.

The frequency with which the electromagnetic field fluctuates significantly affects the depth of penetration of the electromagnetic wave into a biological object.

The reason lies in the commensurability with different physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, EMF wavelength λ = 33 cm, and at f = 2450 MHz, EMF wavelength λ = 12.2 cm. (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

The reason lies in the commensurability with different physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, EMF wavelength λ = 33 cm, and at f = 2450 MHz, EMF wavelength λ = 12.2 cm. (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with the sodium salt of Chlorin-e ₆ and ascorbic acid are 3-4 times higher than its content in healthy tissues, respectively, its electrical conductivity differs so many times, i.e. the ability of tumor tissues to conduct an electric current is due to the presence of an acidic electrolyte in tumors, free charge carriers - electrically charged particles, which, under the influence of an external electric field in the tumor, create a conduction current.

Another important parameter of dielectric and semiconductor materials, which tumors are, are dielectric losses, they serve to determine the electrical power spent on heating dielectrics and semiconductors in an electromagnetic field. In the reference literature, to characterize the ability of a dielectric to absorb the energy of an alternating electric field, the tgδ of the dielectric loss angle and the dielectric constant ε are used. The physical meaning of tgδ consists in the presence of dielectric losses leading to a phase shift between current and voltage, where the angle between them becomes less than 90 ° by the value, the quantitative losses of wave energy are proportional to the dielectric losses ε, tanδ.

Conductivity losses in dielectrics with low specific volume resistivity, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with the changing wave electromagnetic field, converting electrical energy into thermal energy. Tumor tissues are maximally saturated with the sodium salt of Chlorin-e ₆ and AK, in this case they are semiconductors containing several times more charged ions in comparison with the surrounding healthy tissues and, accordingly, their heating rate is many times higher than that of the surrounding healthy tissues at the same time ... In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of polar water molecules in the direction of the lines of force of the electric field. Intramolecular friction arises, which once again increases the heating of tumor tissues.

The specific power of dielectric losses per unit volume of the dielectric is called dielectric losses, which can be calculated by the formula:

Ore = E ² f εtgδ, 10 ^-2 W / cm ³ .

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an electric field. To do this, it is necessary to know ε and tgδ of tumor and healthy tissues, and thus very accurately calculate the heating rate to a given heating temperature of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective absorption of the sodium salt of Chlorin-e ₆ and AA by tumor tissues leads to their selective heating of tumors and electromagnetic photoluminescence to a higher temperature of 50 ° С when heating the surrounding healthy tissues to a temperature of 40 ° С during the same time, which leads to inactivation of tumor tissues and their subsequent destruction, which then, within a few days, are painlessly removed by the body. The rate of heating by the wave energy of the electromagnetic field depends on the power of the dielectric generators and magnetrons.

Knowing the specific power of Ore released in a biological object, taking into account ε and tgδ of tumor and healthy tissues, it is possible to very accurately calculate the heating rate to a given heating temperature of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF), according to the formula:

Rood _op = E f ε ² _{OP OP} tg δ, 10 ^-12 W / cm ³

With the oscillatory power of the electromagnetic field generators of 700-850 watts, 200-300 grams of tumor tissues can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors and their heating temperature is determined by the formula:

where Co is the heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ - heating temperature difference;

t - heating time, sec.

This formula allows you to select the required total specific power of Ore about for HF and microwave heating of tumor tissues Ore up to a given heating temperature difference and the specific power Ore zd released in healthy tissues, determined by the general formula:

Rud _about = Rud _op + Rud _zd

Then the specific power in the field of irradiation, taking into account the dielectric properties:

Rood _on = (E ² f ε tgδ _{op op} + E ² f ε _{zd zd} tgδ) 10 ^-12

Knowing the dielectric properties ε tumor tgδ _{op op} and healthy tissue ε _zd tgδ _zd can determine by calculation of ΔT to a heating temperature required predetermined temperature and to determine the heating time t and the total power density _of Rood, the irradiated region. (Table 2)

Similarly, knowing the dielectric parameters εtgδ and the specific density of tumor tissues saturated with electrophotosensitizers in biological objects γ g / cm ³ , it is possible to calculate by calculation the specific power released in the tumor tissues Rud op, and determine the set temperature and calculate the time of their heating with HF and microwave energy, according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells with HF and UHF energy, intended for the local treatment of cancer patients with tumor tissues, for their hyperthermia in all organs of the human body, characterized by the fact that before treatment a person is transferred to a protein diet for 3 days for maximum saturation of cancer cells with an electron-ionic solution of the sodium salt of Chlorin-e ₆ intravenously in a megadose of 3.1 mg / kg of human weight and ascorbic acid orally in a megadose of 500 mg of ascorbic acid is taken orally, for their maximum accumulation in tumor tissues, respectively 3-4 times higher than in healthy people, after 72 hours after taking the sodium salt of Chlorin-e ₆ and ascorbic acid, 4 hours before treatment, selective hyperthermia of tumor tissues with microwave energy is carried out in accordance with the depth of their location and the depth of penetration of the electromagnetic wave into the human body 6.1 centimeters, with a permitted frequency f = 2450 MHz, with a total heating rate about tumor tissues at an allowed frequency of 0.010 ° С / sec, for 216 sec until the heating temperature of tumor tissues is 58 ° С, while heating healthy tissues not higher than 40 ° С.