RU2739196C2 - Method for initiating death of tumor cells with succinic acid and hf- and uhf energy of wave radiation - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to a method for initiation of tumor cell death, intended for complex treatment of oncological patients having tumor tissues in all organs of human body, by hyperthermia HF- and UHF energy, characterized by the fact that person for 3 days is transferred to a non-carbohydrate diet to create glucose starvation and subsequent maximum saturation of oncological cells with electron-ion solution of succinic acid in a disposable megadose of 3000 mg and after 2.5 hours with maximum accumulation of the preparation in tumor tissues is 3–4 times higher than in healthy, selective hyperthermia of tumor tissues is performed with high-frequency energy in accordance with the depth of their location and the penetration depth of the electromagnetic wave into the human body of 1100 cm, at the allowed frequency f=13.56 MHz, with total heating rate of tumor tissues 0.03 °C/s, for 300 s to temperature of tumor tissues 45 °C, when heating healthy tissues no more than 40 °C.

EFFECT: said method results in thermal death of tumor tissues due to high dielectric contrast of the tumor and increasing difference in its conductivity (dielectric loss) relative to surrounding healthy biological tissues, which reaches multiple values for various tumors.

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Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects with succinic acid and the energy of wave HF and UHF radiation, known as HF and UHF hyperthermia.

In medicine, hyperthermia is referred to as a significant increase in the temperature of the human body over 40 ° C. Hyperthermia for cancer treatment has been used as early as half a century ago. The German physician von Ardenne opened a "thermal" clinic in a water bath for hopelessly oncological patients, whom he heated to 42 ° C. After such a procedure, no more than 17% of people survived, but they were completely cured. The rest died, unable to withstand such high temperatures. This technology is still used in the United States, where the human body is heated to 42.5 ° C, with its subsequent return to life. This treatment technology can be effectively used for selective heating of oncological tissues with HF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

The method of initiating the death of tumor cells by the electromagnetic energy of HF and UHF wave radiation consists in the complex simultaneous action of succinic acid and wave irradiation with HF and UHF electromagnetic energy. After oral administration of a solution of succinic acid inside a person, in order to preserve it in a significant amount in the tumor tissues of the body, it takes time for its maximum accumulation in tumor tissues for 0.5-2.5 hours. The maximum content of succinic acid in tumor tissues occurs during this period of time, and it is 3-4 times higher in tumor tissues than in healthy ones due to selective absorption of acid by tumor tissues.

Treatment of oncological diseases with succinic acid is recommended, which is effectively absorbed by the surfaces of tumor cells, saturating them with oxygen, capable of penetrating tumor cells through cell membranes and destroying tumor cell mitochondria and suppressing the growth of tumor tissues by transferring them to oxygen aerobic nutrition, without damaging normal cells. Succinic acid has a unique effect: it accumulates in exactly those areas that need it, ignoring healthy tissues. Succinic acid receptors do not have significant toxic or side effects on animal or human cells and are selected from the group of these compounds consisting of succinic acid.

As the research of Professor of the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences M.N. Kondrashova, the energetic power of the ATP synthesis process during the oxidation of succinic acid is significantly higher than during the oxidation of any other substrate. That is why many energy-dependent, that is, energy-consuming processes, for example, the accumulation of calcium ions and the provision of biosyntheses with hydrogen, even in isolated mitochondria, can proceed only during the oxidation of succinic acid. The works of the school of M.N. Kondrashova showed that in nature there exist and, if necessary, are activated additional pathways for the formation of succinic acid. In particular, such an additional "injection" of succinic acid in a healthy person occurs during intensive work and during the recovery period after exertion, when the need for rapid ATP reproduction is especially high.

During hypoxia, the respiratory chain of mitochondria cannot accept hydrogen from any other substrate, except for succinic acid. After all, it is during its oxidation that hydrogen enters the part of the respiratory chain much closer to oxygen. At the same time, even with deep hypoxia, the ability to accept hydrogen remains on the site. In this case, the oxidation of succinic acid in mitochondria remains one of the few sources of ATP. Additional intake of succinic acid can significantly help the body's vital functions.

The succinic acid that is formed in the mitochondria instantly burns out in the same place, therefore the current - stationary concentration of the succinic acid present in the tissues does not exceed 10-20 mg / kg of tissue mass at each moment of time and, as a rule, does not leave the mitochondria. Outside the mitochondria, outside the cell, it is practically absent in the bloodstream. It appears outside the mitochondria during severe anaerobiosis (complete absence of oxygen) or during deep hypoxia in some area of tissue.

How does amber work? and why it acts healthily is a question unknown to this day. Amber inhibits the growth of various tumors. This is quite accurately established empirically and does not contradict modern scientific views on the nature of cancer. Succinic acid prevents carcinogens from causing genetic disruptions that lead to the unrestrained multiplication of cancer cells by normalizing energy metabolism in cells, thus delaying their division.

The proposed complex of dehydrosuccinic acid salts of succinates, obtained as a result of the oxidation of the YC of the drug "Succinic acid", is oxidized by hydrogen peroxide in an alkaline medium, the reaction is catalyzed by iron heme, and causes chemilumination under the action of photowave high frequency and microwave hyperthermia, with active release of singlet oxygen. If an oxidizing agent - hydrogen peroxide is added to an alkaline solution of cancer cells, then a glow occurs. In the presence of catalysts, this glow intensifies and becomes brighter. The role of catalysts for a solution of dehydrosuccinic acid of succinate salts obtained as a result of the oxidation of UC is performed by blood iron hemin and various sodium compounds. These chemical activators of chemiluminescence enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. The observation with this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is dehydrosuccinic acid, obtained as a result of the oxidation of the YaK drug "YaK" in the presence of oxygen radicals. Under the action of an oxidizing agent - hydrogen peroxide radicals, succinates are formed, which react with a superoxide radical that forms an internal peroxide (dioxide), which leads to the formation of excited molecules of the YAK drug. The transition of these molecules to the ground initial state is accompanied by emission of a quantum of light. Hydrogen peroxide AK is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, it is a relatively harmless compound, but in the presence of metal ions of variable valence iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide Н ₂ О ₂ , a destructive hydroxyl JOH radical, causing mutations and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl group of enzymes activates molecules, and actively enters into a chemical reaction with it, which leads to a bright glow of tumor tissues.

When placed in a variable electromagnetic field of high intensity and frequency of various biological bodies, they also begin to emit a characteristic radiance of varying intensity and colors, by which one can judge the properties of the object under study. The method of "high-frequency photography" (the Kirlian effect, Kirlianography in honor of the inventor V.Kh. Kirlian) is now widely known in Russia and abroad as a method of experimental research of electromagnetic fields and bioenergetic interactions. But the research of the glow of biological objects in a variable electromagnetic field of high frequency is of the greatest scientific and practical interest. explained by the photoelectromagnetic effect of photowave radiation and luminescence of biological objects.

In accordance with modern concepts aqueous solutions of alkalis and acids in a human body is considered as the associated liquid consisting of separate associated elements - neutral clusters and cluster ions of the general formula (H ₂ O) _n, [(H ₂ O) _n] ^+, [( H ₂ O) _n ] ^- , [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ], etc. .d. where the number of hydrogen-bonded water molecules can reach n times, according to some authors, under the influence of HF and microwave energy, hundreds and even thousands of units. These effects respectively alter the electrical conductivity and biophotoluminescence of biological tissues. A change in the position of one structural element (water molecule) under the influence of any external factor or a change in the orientation of the surrounding neighboring water molecules in the cells provides high sensitivity of the entire information system of water to various external influences (electromagnetic, thermal, sound fields, bioinfluence, etc.). In addition, in water clusters, due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, the proton (H ⁺ ) can migrate according to the relay mechanism, leading to delocalization of the proton within the cluster providing the release of singlet oxygen with a characteristic bright glow that kills cancer cells. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-information, etc. In addition, aqueous solutions of various chemical elements are a source of superweak and weak alternating electromagnetic radiation. In this case, the induction of an external electromagnetic field can occur, causing resonant effects of combination (superposition) of external electromagnetic fields with their own fields in biological objects with photowave radiation, capable of changing the structural and informational characteristics of biological objects, 80-90% consisting of water solutions with various chemical impurities and cause their photoluminescence.

Under the influence of a high-frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, excitation, polarization and ionization of N ₂ , H ₂ , O ₂ and CO ₂ molecules occur. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of an object saturated with various chemical solutions, can color the glow crown in different colors. The shape of the glow crown, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body, granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells, release reactive forms of singlet oxygen contained in super axid radicals, hydrogen peroxide H ₂ O ₂ , and the hydroxyl radical JOH in this case, weak chemilumination is observed, which is enhanced multiple times in the presence of D-AK and AK with HF and microwave irradiation. These effects are also greatly enhanced by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the metachondria. This effect of electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nikola Tesla, when exposed to pulsed high-frequency energy of vessels with liquids capable of emitting light and luminescent lamps, which, without being connected to electrical wires, shone with bright light in the hands of Nikola Tesla, with which he also juggled, which caused genuine delight among the audience, while at that time inexplicable by nature, a phenomenon that only Nikola Tesla knew.

In biology, these factors are called collective luminescence stimuli that change the state of blood and tissue phagocytes and their ability to increase the release of reactive oxygen species, and, accordingly, the protective functions of cells.

In cancer cells, aerobic respiration is absent in mitachondria and is replaced by glycolysis. UC, upon entering the oncocell, inhibits glycolysis and transfers it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely disable glycolysis in tumor cells, it is necessary to completely exclude the access of glucose, or so that UC prevails over glucose in the substrate. In healthy cells, in any amount in cytazole, it exhibits protective antioxidant properties. In cancer cells, with an excess of it, it stimulates the oxidation processes, due to their enrichment with bivalent oxygen, which, when it is overabundant, has a toxic effect on the cancer cells.

It can be argued that there would be even fewer side effects, and the effect is higher, if treatment with powerful doses of UC was based on the background of a complete overlap of the intake of carbohydrates-glucose, as UC's competitors in cancer cells. To do this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract are converted into glucose, which is extremely necessary for the nutrition of cancer cells. With this introduction of cancer cells into artificial glucose "starvation", then a person needs to inject high single doses of YaK. When succinic acid enters the blood vessels of the tumor, which has a large branched network with thin peripheral vessels and a low speed of blood flow in them, during HF and microwave heating it decreases even more at their high temperature of 45 ° C due to blood thickening, which is even more effective , leads to blood coagulation in the vessels of tumor tissues, not allowing them to cool down, due to the absence of a closed circulatory system. In the main human organs, rich in blood vessels, closed in the main circulatory system, there is a cooling of borderline healthy tissues exposed to HF and UHF hyperthermia. A "hungry" tumor is maximally saturated with ascorbic and succinic acids, several times higher than in normal healthy tissues, stimulates the formation of hydrogen peroxide and ionic salts of succinates, in a sufficiently large amount in the interstitial fluid. It is this chemical compound that is formed during the interaction of succinic acid and the internal environment of the body. Peroxide and ionic salts of succinates with formed fumaric acid is a factor or hormone that stimulates the mechanisms of self-destruction and death of cancer cells. The formation of sufficient doses of hydrogen peroxide and other lipid peroxides around and inside the cancer cells and their apoptosis is possible only with a sufficiently large amount of succinic acid intake. Under these conditions, UC can act as an antioxidant or prooxidant, i.e. an oxidizing agent, including showing a destructive rather than a creative property of cancer cells. This is very important in the energy of cells. Therefore, UC can be designated as a switch of metabolism, which accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in mitochondria and is replaced by glycolysis. UC, upon entering the oncocell, inhibits glycolysis, but is unable to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely disable glycolysis in tumor cells, it is necessary to completely exclude the access of glucose, or so that UC prevails over glucose in the substrate. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In cancer cells, with an excess of it, it stimulates the oxidation processes, with the formation of hydrogen peroxide and lipid peroxides, which, when they are in excess, have a toxic effect on onco cells.

Succinic acid is actively imported into the endoplasmic reticulum (EPR) (Endoplasmic reticulum, consisting of membranes and mitachondria and setting the direction and active transport of substrates in onco cells) cells. It should be noted that energy processes in cancer cells occur in mitachondria and endoplasmic reticulums. It is here in mitachondria and ER that UC accumulates and the environment of the oncocell in this place differs significantly from ordinary cells. From this moment, the destructive effect of the UC on the oncocell begins. At this time, a "hungry" cancer cell can accumulate UC in itself many times. perceives it on its membrane transporters for glucose and it transfers the oncocell from glycolysis to anaerobic nutrition. Since there are many times more glucose-consuming receptors in the oncolet than in healthy ones, although the transport systems for the delivery of glucose and UC into the cell are common, this is the "Trojan horse" for oncocells. Thus, one can very simply deceive the oncocells and inject UC into them, with the solution of the problem of supplying megadoses of UC, and then the phenomenon of cancer cell death will be greatly enhanced.

A "hungry" tumor is maximally saturated with D-YaK, 3-4 times higher than in normal healthy tissues, in a sufficiently large amount on membranes and interstitial fluid. A "hungry" tumor in the absence of glycolysis is saturated for a maximum of 0.5-2.5 hours, and is many times higher than in normal healthy tissues, stimulates the formation of macrophages and T-lymphocytes under the action of the enzyme ferrachelatase, in a sufficiently large amount on membranes and interstitial fluid. It is this chemical compound that is formed in the process of interaction of YaK and D-YaK and hydrogen peroxide in the internal environment of the body. Under the action of an oxidizing agent of lipoperoxide radicals and the formation of hydrogen, significantly enhanced by the temperature and action and additional photodynamic action of the HF and UHF electromagnetic fields, active hydrogen and oxygen radicals are formed, which then react with superoxide succinate radicals, which accelerate and form internal peroxide (dioxide) , Н ₂ О ₂ with their hyperthermal decomposition, HF and microwave energy. In this case, there is a multiple increase in the formation of excited oxygen molecules. The transition of molecules of internal peroxide and D-AA from the excited state to the ground state is accompanied by the emission of light quanta and a strong glow. As a result of these chemical reactions associated with a high release of reactive forms of hydrogen and oxygen and organic free radicals, significantly enhanced temperature action and additional photodynamic action of electromagnetic fields of HF and microwave photowave radiation, oncological cells are burned out.

The method of "selective starvation" of superficial and deeply located onco cells in the human body, by subsequent administration or administration of various sensitizers, for selective maximum saturation of tumor cells with highly electrically conductive electron-ion solutions of electrophotosensitizers with a maximum separation of the electrophysical properties of tumor and healthy tissues with subsequent selective action on them electromagnetic fields of high frequency in combination with other methods - this is the most relevant scientific and practical direction in the fight against oncological diseases.

The study of biophysical and biochemical mechanisms determine two concepts of cancer cell death, one assumes the significance of D-UC, and the other UC, which in both cases leads to a clear death of onco cells. The main task for researchers remains to enhance as much as possible the effect of selective absorption of D-UC and UC by cancer cells with simultaneous subsequent high-frequency irradiation of cancer cells, in order to increase the effectiveness of treatment up to 100%.

A number of researchers argue that succinic acid, which is a prooxidant that neutralizes free radicals, kills cancer cells in the blood and tissues without affecting healthy ones, due to the damage caused by local oxidative stress, as a result of oxidation of cellular DNA and depletion of adenosine triphosphate (ATP) - energy source of the cell. Succinic acid, among other accompanying molecules, due to aggressive action, causes a malfunction of the functioning of a certain enzyme responsible for the "nutrition" of cells of malignant tumors. YaK is a representative of intermediate acids that can accumulate in the cytosol of cells.

Tumor cells accumulate, in contrast to normal cells, a significant amount of homocysteine teolactone (HTL). Before homocysteine is inserted into a protein, it becomes a biological marriage, in the form (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone is practically not formed from it, but transformation into a cancer cell requires a significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein that synthesizes the machine of the cancer cell works at full capacity, so it is more often mistaken. Teway found that interaction with an organic acid, this substance forms the highly toxic 3-mercaptopropionaldehyde (MPA). When dehydrosuccinic acid enters a cancer cell saturated with HTL, MRA is formed, which, under the influence of HF and microwave energy, kills the cancer cells. Destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer from it under the influence of succinic acid.

The study of biological objects shows their connection with free radical oxidation in the body, proceeding without the participation of enzymes, due to the reduction of molecular oxygen to its active forms: superoxide onion radical, hydroxyl radical, singlet oxygen. The main source of these compounds is the autooxidation of lipids, mainly unsaturated fatty acids (folic acid), in which free radicals are released under the influence of temperature and high-frequency and microwave fields, interacting with oxygen to form peroxides. During the recombination of these peroxides, light quanta and singlet oxygen are released, which destroy tumor cells. Researchers have established a similar fact under the action of short-term electrical impulses that stimulate the release of reactive oxygen species and increase the permeability of cell membranes. These effects of lipid peroxidation are observed in tumor tissues with prolonged oxygen deficiency.

The more succinic acid in the oncocell, the more peroxidized lipids and peroxides are formed there, in comparison with healthy cells. An excess of peroxides triggers the death of cancer cells by HF and UHF fields by rapidly heating cancer cells to 45 ° C. Due to the rapid oxidative breakdown of succinic acid and oxidized lipids. Under the influence of temperature and RF and microwave fields, interaction with free oxygen occurs with the formation of peroxides. During the recombination of these peroxides, light quanta and singlet oxygen are released, which destroy tumor cells. In this case, it can be argued that in the treatment of cancer with oxidized succinic acid, obtained as a result of the oxidation of UC, and the simultaneous exposure to high-frequency and microwave energy in the blood and human organs, a 100% therapeutic effect is observed.

It has already been proven that such an effect is possible by the example of disinfection of biological objects from viral, fungal and bacterial infections with high-frequency and microwave energy. Numerous studies conducted by us in the Krasnoyarsk State Agrarian University and the VIZR in St. Petersburg have confirmed 100% efficiency of disinfection of vegetable seeds and living biological objects saturated highly conductive electronic-ionic solutions of microelements of HF and UHF energy against viral infections, which have a similar origin with cancer cells. The results are confirmed by the following works: A.S. No. 563938 USSR. Method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bul. No. 25. Certificate of the USSR No. 950214. Method of pre-sowing seed treatment / Tsuglenok N.V. - Registered in the registry on 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with HF and UHF energy: guidelines / N.V. Zuglenok. - M .: Agropromizdat, 1989. Guidelines for the use of high-frequency and microwave energy in the preparation of seeds for sowing / N.V. Zuglenok. - M .: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfection of tomato seeds against viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute of Plant Protection (VIZR). - 1989. - T. 71. - S. 49 - 54. Method of disinfection of egg powder. Patent number: 1734632. Published: 23.05.1992 Authors: Tsuglenok N.V., Kolmakov Yu.V. IPC: A23v 5/02. Method of preparing a medium for diluting semen from the manufacturer. Patent number: 1769422. Published: 27.06.1995. Authors: Tsuglenok, Ostashko, Shakhmatov, Silantyeva, Kontsedal.

It has been proven that under the influence of carcinogens, oncoviruses are incorporated into a healthy cell and eventually dissolve in it, turning it into an oncocell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless, they simply are not. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the shallow depth of penetration of the electromagnetic wave of laser emitters does not allow burning deep-seated malignant tumors.

It is necessary to note another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein envelope filled with a mixture of nucleic acids and similarly tumor cells filled with solutions of intercellular fluid determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. HF and microwave heating in tumor tissues significantly increases the mobility of ions and electrons, increasing their electrical conductivity and dielectric losses, which further enhances their selective heating and apoptosis of viral and tumor tissues.

It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects.

This curing effect is explained by the fact that at this time, within 0.5-2.5 hours in normal cells of living biological objects, succinic acid is rapidly pumped into oncological cells, while maintaining a high contrast of the content of succinic in the tumor 3-4 times higher than in healthy tissues. , which significantly increases its concentration and, accordingly, electrical conductivity with a significant change in the dielectric properties of tumors relative to the surrounding healthy biological tissues.

With further hyperthermia, tumor cells saturated with succinic acid are 3-4 times higher than in healthy ones, when taken by a person orally at a dose of 2400 mg. after 2.5 hours, the tumor tissues are treated with photowave radiation of HF and microwave fields, for 300 seconds at a heating rate of 0.03 ° C / sec to the final heating temperature of tumor cells of 45 ° C. at the permitted frequencies of oscillations of the electromagnetic field f = 433 92 MHz, f = 915 MHz or 2450 MHz, This allows the simultaneous effect of succinic acid and HF and microwave hyperthermia on tumor cells with the energy of photowave radiation, destroying them, even undetectable tumor formations located in the deep layers of the biological object.

The biophysical meaning of this method lies in the selective maximum saturation and accumulation of highly electrically conductive electron-ion solutions of electrophotosensitizers in tumor cells and in the maximum separation of the electrophysical properties of tumor and healthy tissues with succinic acid and a significant increase in the difference in the electrical potentials of tumor and healthy cells in the intercellular medium and on the walls of the reticulum. ... The reticulum is an electrical circuit where negative charges are evidently accumulated on one side of the membrane, and positive charges on the opposite side, therefore the reticulum is an electric transporter of glucose and other nutrients of cancer and healthy cells. Consequently, the reticulum is an electrical network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of the metachondria and the energetic operator structures on the outer side of the cell - on the cilia. These proteins, under certain situations in the environment of the cell, being discharged, can give an active signal to the reticulum and metachondria. This changes the balance that exists on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are triggered. One side of the reticulum membrane is connected to one type of entrance to the metachondria, and the opposite side to the exit from it. Thus, a single electrical circuit is created for double active control of the energy of the metachondria. The strength of the electric field on the reticulum keeps the metachondria under control. In this case, the metachondria pull in the charges accumulated on one side of the reticulum membrane and remove the opposite charges to the other side of the reticulum membrane. The charges are thus not mixed and separated. This is important for ion exchange to take place in cells. Externally, the reticulum is similar to the capacitor plates, the more layers of plates, the greater its electrical capacity. Between the gaskets there is a semiconductor saturated with the folic acid polysaccharide complex. This capacitor, i.e. a powerful dense network of tumor sheaths-membranes is very clearly visible through a microscope. The number of membranes in tumor cells is much higher than in healthy ones. Accordingly, the density of tumor tissues and the capacity of the biological electrical capacitor are significantly higher than those of healthy tissues. When charging, negatively charged particles-electrons will be collected on one plate of such a capacitor, and ions, positively charged particles, will be collected on the other. Such a charged capacitor can turn into a current source if disconnected. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which discharges the charge on the metachondria, controlling their activity. Metachondria, in turn, are tuned in such a way that they never allow the charges on the reticulums to drop below a critical level. In cancer cells, the charges inside the metachodria are sharply reduced and the entire regulation system is disrupted. This is the main control rod for the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not primary. As a result of the electrochemical energetics of the cell in the reticulum there is a circulation of substances, where the metachondria are the pump. With a lack of this circulation between the reticules and metachondria, due to electroosmosis, substances are sucked from the outside through the outer membrane and the gateways and polysaccharide pump are opened on it. The medium on the membranes of the reticulum and the liquid substrate is reconstituted due to the excess of negative charges. This is what determines the chemical equilibrium in terms of pH, conjugated buffer chemical electrochemical substances, when the buffer system is discharged or restored. Regulates these processes by charges on the lining of the reticulum and metachondria. Chemical processes, in this case, are simply executors, intermediaries. The outer side of the metachondria provides a voltage dependent anion channel. This voltage maintenance mechanism is called VDAC, which sets the conditions for the work of the reticulum. It is here on the outer side of the membrane that the Hexokinase II enzyme is located, which ensures the utilization of glucose. Separation, disconnection of the work of the outer mitochondrial membrane (VDAC) and Hexokinase II ensures the induction of apoptosis.

The metachondria works by pulling from the reticulum into itself as an electromagnetic pump, which requires power under high voltage. Without this effect, the highest tension of pulling in nutrients into the cell will not be possible. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Cancer cells, unlike normal cells, do not have cilia. This most damaging energy level is absent in cancer cells. Therefore, the only correct way to find a weak spot in the energy of cancer cells and thereby destroy them. Metachondria set the degree of charge for ion pumps on the outer membrane of the cell and for starter structures that hold charges on the reticulum. These sensory structures can be most rapidly damaged and burned out, since metachodria are the most efficient electrochemical furnaces. In the case of turning off the metachondria, the voltage gradient of the cell decreases sharply and the processes take place in onco cells over a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. The oncocell takes not quality, since everything is concentrated on a small area of metochondria, but with a larger number, much larger than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but at the same time the consumption of glucose, which, in this case, is used to feed "hungry" cancer cells succinic acid, with a rich release and content of bivalent oxygen.

The membranes of the reticulum and the nucleus of the cell are the same, and the reticulum, as a capacitor, is contoured to the nucleus with only one side of it - an electron and dumps electrons into the nucleus. Thus, the charge in the reticulum also provides charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a super-powerful electrostatic charge inside the nucleus.

In oncocells, in comparison with healthy ones, the environment is different, supersaturated with succinic acid, which tries to trigger the mechanisms of apoptosis in the oncocell to the maximum. In this case, significant destruction occurs with damage to cell membranes, various organelles, mutation of nucleic acids, inoculation of enzymes, destruction of nutrients and cell death along the path of apoptosis.

A known method for the production of a polysaccharide complex of folic acid, which is a polysaccharide of denitrofolic acid, which allows to enter the cell of folic acid along the path with the participation of the glucan receptor and other hydrophilic alcoholic polymers of folic acid on the cell membrane, as anticancer drugs. (RF patent No. 2280650-LU Weiyu (CN) LIU Ming (CN) PAN Yun (CN)). This complex has pro-tumor activity, provides for long-term treatment and does not guarantee 100% cure, since folic acid without a transporter polysaccharide is also actively involved in the nutrition and active division of cancer cells.

There is a known method of destruction of cancer cells with microwave - irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the value of the resonant frequency is absorbed by the tumors. After that, a similar effect is carried out on healthy tissues bordering with the tumor and the value of the resonance frequency of absorption of these healthy tissues is determined. Simultaneously with hyperthermia, the values of resonance frequencies of energy absorption by tumors and healthy tissues are monitored, and when the values of resonance frequencies approach, energy absorption by tumors and healthy tissues is judged on the effectiveness of treatment. This method improves the efficiency of tumor treatment by microwave hypothermia when heated to 43 ° C.

The main disadvantage of this method is the shallow depth of penetration into biological tissue, amounting to several millimeters, due to the strong absorption of microwave radiation, with a wavelength of 1.3-2 cm, by water molecules and a small difference in heating of tumor and healthy tissues.

There is a known method for the destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N5 / 02, A61N5 / 6). The essence of the invention includes the introduction of ferromagnetic particles into the tumor localization area, followed by induction local heating, in the temperature range from 42 ° C to 45 ° C, in the course of time, determined by the type of tumor, its size, localization and the type of ferromagnetic particles selected for induction heating, while heating is carried out only at the moments of decrease in tissue blood filling, i.e. at the moments of expiration and diastole of the patient's heart. The heating range is controlled by a microwave deep thermometer, and heating is carried out automatically, with the help of a computer, in the biocontrol mode, according to the algorithms of the mathematical model of fluctuations in the thermal conductivity and heat capacity of the tissue, the hysteresis of heating and heat removal.

The main disadvantages of this method are the low localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in different spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, Serdar Deger, Stefan Loening, Wolfgang Lanksch, Roland Felix. Journal of Magnetism and Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on thermolysis of magnetic nanoparticles injected into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions through cell membranes, as well as generate inductive alternating electric fields that affect the operation of neural networks in the human body, associated with heating not only magnetic particles, but all cells. located in the area of injection of magnetic particles, and a strong spatial inhomogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

A known method of photodynamic destruction of tumors, including the intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength coinciding with the absorption band of the photosensitizer (see Photodynamic therapy / Ed. TJ Dougherty / J. Clin. Laser Med Surg. 1996, Vol. 14, P 219-348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of the accumulation of the photosensitizer in tumor cells as compared to healthy cells, which is associated with a high density of blood vessels in a tumor as compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, dye molecules pass into an excited electronic state, and upon collision with oxygen molecules dissolved in biological tissue, they transfer it from an unexcited to an excited electronic singlet state, with a typical lifetime of several microseconds. During this time, singlet oxygen molecules, having traveled a characteristic path commensurate with the size of cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, the destruction of cells occurs only during exposure to laser radiation in the spatial area of laser irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. Used in practice photosensitizers-phthalacionines. porphyrins, chlorins have absorption bands of photosensitizers in the ultraviolet or visible region of the spectrum, and the lasers used cannot effectively penetrate to a depth of less than a few millimeters. In addition, the photodynamic method has a low contrast of the accumulation of photosensitizers in cancer cells.

The known method of close-focus X-ray therapy with a total focal area of 100-120 Gy and remote gamma therapy with radiation destruction of malignant cells with a total focal area of 30-40 Gy (see Sh.Kh. Gantsev. Oncology, M .: Medical Information Agency. 2004, pp. 190-204; Stephen J., Withrow E., MacEwen G. Smal animal clinical oncology - 2001, p. 305-308).

However, this method, despite its prevalence, has the following disadvantages. In the treatment of certain types of malignant neoplasms, for example melanoma, using gamma remote therapy, even in combination with immunotherapy, experience shows that it leads to 75-90% of tumor recurrence, and after 2-6 months metastases occur.

The closest to the claimed one is a neuron - a capturing method for selective destruction of melanoma (see V.N. Mitin, N.G. Kozlovskaya, AM Arnopolskaya Neuron-capture therapy of tumors of the oral cavity in dogs. All-Russian veterinary journal. 2006. No. 1, p. . 9-10).

The method includes intravenous injection of L-borphenylalanine into the blood, which selectively accumulates in a certain tumor - melanoma, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, there is a selective accumulation of L-borphenylalanine in melanoma cells. When a spatial zone comparable to a tumor containing L-borphenylalanine is irradiated by a beam of slow neurons obtained through a neuron guide from a nuclear reactor, melanoma cells are destroyed due to induced secondary local boron radiation.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced by using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to service.

3. Long-term irradiation of patients within an hour while monitoring the cardiovascular system.

4. Application of general anesthesia.

The objective of the present invention is the local selective destruction of malignant tumors deeply located in human biological tissues, pre-selectively saturated for 2.5 hours with succinic acid, with simultaneous selective HF and microwave heating of tumors saturated with succinic acid, heated to a temperature of 45 ° C at a rate heating 0.03 ° C / sec for 300 sec. and during the secondary reverse heating of the boundary layers of healthy tissues due to thermal conductivity from tumor tissues, after turning off the HF and microwave energy supply, from the boundary layers of tumor tissues, due to contact heat transfer of high temperature to healthy tissues, with minimal destruction of the surrounding healthy cells of biological tissue, possibly degenerating into tumor cells.

The physical nature of microwave radiation is the same as that of light. The difference is only in the frequency with which electromagnetic oscillations occur with the corresponding wavelength, which is the same, since the latter is related to the frequency by the ratio:

,

,

where λ is the wavelength,

c is the speed of propagation of an electromagnetic wave,

f is the frequency of oscillations of the electromagnetic field.

The frequency with which the electromagnetic field fluctuates significantly affects the depth of penetration of the electromagnetic wave into a biological object. Table 1.

The reason lies in the commensurability with different sizes of physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, the EMF wavelength λ = 33 cm, and at f = 2450 MHz, the EMF wavelength λ = 12.2 cm (Table 1).

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects. Such equipment is manufactured by the FGUP "NII" "ISTOK" company in Fryazino, Moscow region. Yacht - 5 operates at frequencies at f = 13.56 MHz, at an EMF wavelength λ = 22 m, and at f = 40.68 MHz, an EMF wavelength λ = 7.4 m. The possible penetration depth of EMF into biological tissues is these frequencies are more than 1 m. These installations are intended for human hyperthermia in its entirety. Yacht - 4 operates at frequencies at f = 433.92 MHz, wavelength

EMF λ = 69 cm. The possible penetration depth of EMF into biological tissues at this frequency is up to 69 cm. They are used for local human hyperthermia. Yacht-3 operates at frequencies at f = 915 MHz, the EMF wavelength λ = 33 cm, the possible penetration depth of EMF into biological tissues at this frequency is up to 33 cm. Yacht-2 operates at frequencies at f = 2450 MHz, the EMF wavelength λ = 12.2 cm. These settings are also intended for local human hyperthermia. The possible penetration depth of EMF into biological tissues at this frequency is up to 12.2 cm.

Tumor tissues saturated with succinic acid are several times higher than its content in healthy tissues, respectively, the electrical conductivity of the tumor differs so many times, i.e. the ability of tumor tissues to conduct electric current and absorb the energy of the electromagnetic field. In the presence in tumors of the acidic electrolyte D-YaK, free positively charged ions, which, under the influence of an external electric field in the mitachondria of the tumor, create conduction current and displacement currents in negative charges on the reticulums of the cell membranes, i.e. shells of cancer cells and in their nuclei.

Another important parameter of dielectric and semiconducting materials, such as tumors, is the dielectric loss of water, which is greater in tumor tissues than in healthy ones and it determines the loss of electrical power spent on heating tumor tissues that have dielectric and semiconducting properties in the HF and UHF electromagnetic field. In the reference literature, to characterize the ability of a dielectric to absorb the energy of an alternating electric field, the tgδ of the dielectric loss angle and the dielectric constant ε are used. The physical meaning of tanδ consists in the presence of dielectric losses leading to a phase shift between current and voltage, where the angle between them becomes less than 90 ° by the amount of quantitative losses of wave energy are proportional to the dielectric losses ε tanδ.

Conductivity losses in dielectrics with low specific volume resistivity, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with the changing wave electromagnetic field, converting electrical energy into thermal energy. Tumor tissues in this case are semiconductors containing several times more charged ions in comparison with the surrounding healthy tissues and, accordingly, their heating rate is several times higher than that of the surrounding healthy tissues at the same time in a uniform HF and microwave electromagnetic field. In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of polar water molecules in the direction of the lines of force of the electric field. Intramolecular friction arises, which once again increases the heating of tumor tissues.

The specific power of dielectric losses per unit of tumor volume can be calculated using the formula:

Ore = E ² f ε tgδ, 10 ^-12 W / cm ³

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an alternating and electromagnetic field. To do this, it is necessary to know ε and tgδ of tumor and healthy tissues, and thus very accurately calculate the heating rate to a given heating temperature of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective absorption of succinic acid by tumor tissues and simultaneous exposure to high-frequency and microwave energy leads to selective heating of tumors to a temperature of 45 ° C in 300 seconds, while heating the surrounding healthy tissues to a temperature of 40 ° C during the same time. When tumors are heated to 45 ° C, succinic acid decomposes into peroxide, with the release of a hydroxyl radical, singlet oxygen, and light quanta are released. Sources of chemiluminescence and free radical oxidation are excited molecules of oxygen dimers, ketones, cyclic hydroperoxides, oxolates and aldehytes, biogenic amines. The same emission of fatons is observed during the decomposition of all peroxides (intermediate products of reactions with molecular oxygen). Free radical oxidation is inhibited in the body, due to natural antioxidants, which are divided into hydrophobic antioxidants (tocopherols, flavins, carotenoids, steroids) and hydrophilic (sulfhydryl compounds of SH-protein groups, ascorbic acid) and phases. Thus, chemiluminescent activity may indicate excessive free radical oxidation in the body, under the influence of the energy of high HFC and microwave energy currents. In this case, succinic acid is a very strong antioxidant. This phenomenon of chemiluminescence was observed by researchers earlier, under the action of short-term electrical impulses that stimulate the release of reactive oxygen species and an increase in the permeability of cell membranes, which leads to the inoculation of tumor tissues and their subsequent destruction, which then, over several days, with the help of further intake of succinic acid, painlessly excreted by the body within 2-4 weeks. The rate of heating by the wave energy of the electromagnetic field depends on the power of the dielectric generators and magnetrons.

Knowing the specific power of Ore released in a biological object, taking into account ε and tgδ of tumor and healthy tissues, it is possible to very accurately calculate the heating rate to a predetermined heating temperature of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF). according to the formula:

Ore _op = E ² f ε _op tan _op δ, 10 ^-12 W / cm ³

With the oscillatory power of the electromagnetic field generators of 700-850 watts, 200-300 grams of tumor tissues can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors and their heating temperature is determined by the formula:

,

,

where Co is the heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ - heating temperature difference;

T - heating time, sec.

This formula allows you to select the required total specific power of Ore about for HF and microwave heating of tumor tissues Ore op up to a given heating temperature difference and the specific power of Ore zd released in healthy tissues, determined by the general formula:

Rud about = Rud op + Rud zd

Then the specific power in the field of irradiation, taking into account the dielectric properties:

Ore ob = (E ² f ε _op tan δ _op + E ² f ε _bld tg δ _bld ) 10 ^-12

Knowing the dielectric properties of tumor ε _op tgδ _op and healthy tissues _εb tg _δb , it is possible to calculate the temperature of their heating ΔT to the required specified temperatures and determine the heating time t and the total specific power Rud about the irradiated area. (Table 2)

Similarly, knowing the dielectric parameters εtg δ and the specific density of tissues of biological objects γ g / cm ³ , it is possible by calculation to find the specific power released in the tumor tissues Rud op, and determine the set temperature and calculate the heating time, according to the above formulas of tumor tissues by various electrophotosensitizers and RF and microwave energy.

Claims (1)

A method for initiating the death of tumor cells, intended for the complex treatment of cancer patients with tumor tissues in all organs of the human body, by means of their hyperthermia with HF and microwave energy, characterized by the fact that a person is transferred to a carbohydrate-free diet for 3 days to create glucose starvation and the subsequent maximum saturation of cancer cells with an electron-ionic solution of succinic acid in a one-time megadose of 3000 mg, and after 2.5 hours with a maximum accumulation of the drug in tumor tissues 3-4 times higher than in healthy ones, selective hyperthermia of tumor tissues with HF energy is performed in in accordance with the depth of their location and the depth of penetration of an electromagnetic wave into the human body of 1100 cm, at an allowed frequency of f = 13.56 MHz, with a total heating rate of tumor tissues of 0.03 ° C / s, for 300 s to a temperature of tumor tissues of 45 ° C, when heating healthy tissues not higher than 40 ° C.