RU2726609C2 - Method for initiating death of tumour cells with hydroxyaluminum trisulfophthalocyanine, succinic acid and hf- and microwave energy of wave radiation - Google Patents

Abstract

FIELD: medicine.SUBSTANCE: invention relates to method of initiating tumour cell death with HF- and microwave energy, intended for local treatment of cancer patients having tumour tissues in various organs of human body, characterized by that before treatment, person for 3 days is transferred to a non-carbohydrate diet, to create glucose starvation and subsequent maximum saturation of oncocells with an electron-ionic solution of hydroxyaluminium trisulfophthalocyanine intravenously in megadose of 0.81 mg/kg of body weight of the Photosens preparation and peroral human intake of succinic acid in megadose 3000 mg, for their maximum accumulation in tumour tissues, respectively, 1.5–1.8 and 3–4 times more than in healthy in 2–8 and 0.5–2.5 hours before physiotherapy, selective hyperthermia of tumour tissue with UHF energy is performed in accordance with the depth of their location and depth of penetration of the electromagnetic wave into the human body of 6.1 centimetres, at the resolved frequency f = 2450 MHz, with a total heating rate of the tumour tissues of 0.066 °C/s for 195 s to the tumour tissue heating temperature of 49.5 °C, while heating healthy tissues not higher than 40 °C.EFFECT: disclosed is a method for initiating the death of tumour cells with hydroxy aluminium of trisulfophthalocyanine, succinic acid and HF- and microwave energy of wave radiation.1 cl, 1 ex, 2 tbl

Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects of hydroxyaluminium trisulfophthalocyanine of the preparation "Photosens", contained in the medical preparation "Photosens" with succinic acid (UC) and the energy of wave HF and microwave radiation, known as HF and UHF hyperthermia.

Known methods for initiating the death of tumor cells by HF and UHF hyperthermia. In medicine, hyperthermia is referred to as a significant increase in the temperature of a person's body over 40 ° C. Hyperthermia for cancer treatment has been used as early as half a century ago. The German physician von Ardenne opened a "thermal" clinic on a water bath for hopelessly oncological patients, whom he heated to 42 ° C. After such a procedure, no more than 17% of people survived, but they were completely cured. The rest died, unable to withstand such high temperatures. This technology is still used in the United States, where the human body is heated to 42.5 ° C, with its subsequent return to life. This treatment technology can be effectively used for selective heating of oncological tissues with HF and UHF energy without significantly increasing the temperature of healthy tissues surrounding the tumor.

The method of initiating the death of tumor cells by the electromagnetic energy of wave radiation consists in the complex simultaneous action of the hydroxyaluminium trisulfophthalocyanine of the Photosens preparation, succinic acid (UC) and high-frequency and microwave energy. The drug Photosense, and YaK dissolves very well in aqueous solutions. After intravenous administration of the Photosense solution, for its accumulation and preservation in a significant amount in the tumor tissues of the human body, time is required for its maximum accumulation in tumor tissues within 2-8 hours and succinic acid within 0.5-2.5 hours. The maximum content of hydroxyaluminium trisulfophthalocyanine Photosens and UC in tumor tissues occurs precisely during this period of time, and it is higher than in healthy tissues, respectively, 1.5-1.8 and 3-4 times, due to the selective absorption of these drugs by tumor tissues. The drug Photosens is excreted and redistributed from normal tissues and the human circulatory system into the tumor in a day, 94% after intravenous administration, and succinic acid is excreted after 2.5 hours. The introduction of the drug Photosense is carried out intravenously (drip) with a 30-minute infusion in a semi-darkened room in a single dose of 0.05-0.8 mg / kg of body weight with preliminary dilution 24 hours before diagnostics and hyperthermia of tumor tissues with high-frequency and microwave energy of wave radiation. Within 8-24 hours after the introduction of Photosens, the patient should be isolated from bright sunlight. The patient is allowed to stay in a room with an artificial light source. Treatment of a person with HF and UHF energy is carried out after the injection of the Photosens preparation and its maximum accumulation in tumor cells after 24 hours and the injection of succinic acid 2.5 hours before HF and UHF irradiation. After this time, selective electromagnetic HF and UHF hyperthermia of tumor cells is carried out for 195 seconds by wave radiation at a heating rate of 0.066 ° C / sec to a final heating temperature of tumor cells of 49.5 ° C. at the permitted frequencies of oscillations of the electromagnetic field f = 13.56 MHz, f = 40.68 MHz, f = 433 92 MHz, G = 915 MHz and f = 2400 MHz,

The concentration of the "Photosens" preparation remains in tumor tissues for 24 hours after its intravenous administration. After 24 hours, after intravenous administration, Photosense is removed from healthy tissues in comparison with tumor ones, and after 2.5 hours of oral administration, succinic acid is removed, its rapid removal from healthy tissues occurs. In other organs and tissues, the concentration of the drug, during this period of time, sharply decreases, which causes maximum damage to the tumor during HF and microwave electromagnetic hyperthermia during this period of time, after which the tumors denature and within 2-4 weeks the decay products of tumor cells are excreted by the body yourself. This results in a high fluorescent tumor contrast and an enhancement of its electrophysical properties, i.e. an increase in its conductivity (dielectric losses) relative to the surrounding healthy biological tissues, reaching a difference of 1.5-1.8 and 3-4 times for various tumors. This allows, during fluorescence diagnostics, to clarify the boundaries of tumors and simultaneously conduct hyperthermia of tumor cells with electromagnetic energy of wave radiation and to identify and destroy, thus, even undetectable tumor formations located in the deep layers of a biological object. The proposed complex of solutions of hydroxyaluminium trisulfophthalocyanine Photosens and succinic acid on the cell membrane is taken by onco cells for glucose.

Succinic acid (succinates) is a substance obtained during the processing of natural amber. It is a completely safe product with a number of beneficial qualities. Received in the form of a white crystalline powder with a taste similar to citric acid. In the body, succinic acid is active in the form of anions and salts called succinates. Succinates are natural regulators of the body. We feel the need for them with increased physical, psychoemotional, intellectual stress, with various diseases. Succinic acid has a unique effect: it accumulates in exactly those areas that need it, ignoring healthy tissues.

For the treatment of oncological diseases, succinic acid is recommended, which is effectively absorbed by the surfaces of tumor cells, reducing hypoxia of tumor cells and saturating them with oxygen using succinic acid, which is able to penetrate into tumor cells through cell membranes and is capable of destroying tumor cell metachondria and suppressing the growth of tumor tissues by transfer them on oxygen aerobic nutrition, without damaging normal cells. Succinic acid receptors have no significant toxic or side effects on animal or human cells and are selected from the group of these compounds consisting of succinic acid.

Succinic acid has no side effects and is practically harmless. Its only unpleasant quality is that it can irritate the stomach lining, so it should not be consumed on an empty stomach. Also, succinic acid has the ability to enhance the therapeutic effect of medicines. Succinic acid acts only where there is a shortage of it, accumulating in damaged areas, that is, its effect is targeted, which is practically impossible to say about any drug.

As the research of Professor of the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences M.N. Kondrashova, the energy capacity of the ATP synthesis process during the oxidation of succinic acid is significantly higher than during the oxidation of any other substrate. That is why many energy-dependent, that is, energy-consuming processes, for example, the accumulation of calcium ions and the provision of biosynthesis with hydrogen, even in isolated mitochondria, can only take place during the oxidation of succinic acid. The works of the school of M.N. Kondrashova showed that in nature there exist and, if necessary, are activated additional pathways for the formation of succinic acid. In particular, such an additional "injection" of succinic acid in a healthy person occurs during intensive work and during the recovery period after exertion, when the need for rapid ATP reproduction is especially high.

During hypoxia, the mitochondrial respiratory chain cannot accept hydrogen from any other substrate, except for succinic acid. After all, it is during its oxidation that hydrogen enters the part of the respiratory chain that is much closer to oxygen. At the same time, even with deep hypoxia, the ability to accept hydrogen remains on the site. In this case, the oxidation of succinic acid in mitochondria remains one of the few sources of ATP. Additional intake of succinic acid can significantly help the body's vital functions.

During high-frequency and microwave heating of tumor tissues, hydroxyaluminium trisulfophthalocyanine of Photosens is oxidized by succinic acid with the release of hydrogen in an alkaline medium, the reaction is catalyzed by iron heme, and causes chemilumination with active release of singlet oxygen. If an oxidizing agent succinic acid with active hydrogen evolution is added to an alkaline solution of cancer cells, then a glow occurs. In the presence of catalysts, this glow intensifies and becomes brighter. The role of catalysts for the Photosens trisulfophthalocyanine hydroxyaluminum solution is carried out by blood iron hemin and various sodium compounds. These chemical activators of chemiluminescence enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. The glow observed in this case is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is the hydroxyaluminium of trisulfophthalocyanine Photosens in the presence of oxygen radicals. Under the action of the oxidizing agent YC - hydrogen radicals, formation occurs, which reacts with a superoxide radical, which forms an internal peroxide (dioxide), which leads to the formation of an excited molecule of hydroxyaluminum trisulfophthalocyanine of the Photosens preparation. The transition of this molecule to the initial ground state is accompanied by emission of a quantum of light. Hydrogen peroxide is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, this is a relatively harmless compound, but in the presence of metal ions of variable valence iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide Н ₂ О ₂ , a destructive hydroxyl radical is formed JOH, which causes mutations and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl group of enzymes causes the activation of molecules, under the action of oxidized succinic acid and hydroxyaluminium trisulfophthalocyanine Photosens actively enters into a chemical reaction with them, and under the action of the electromagnetic field of HF and microwave leads to a bright glow of biological tumor tissues caused by photoelectromagnetic fluorescence.

When placed in a variable electromagnetic field of high intensity and frequency of various biological bodies, they also begin to emit a characteristic radiance of various intensities and colors, by which one can judge the properties of the object under study. The method of "high-frequency photography" (the Kirlian effect, Kirlianography in honor of the inventor V.Kh. Kirlian) is now widely known in Russia and abroad as a method of experimental studies of electromagnetic fields and bioenergetic interactions. But of the greatest scientific and practical interest are studies of the glow of biological objects in an alternating electromagnetic field of high frequency, explained by the photoelectromagnetic effect of photowave radiation and luminescence of biological objects.

In accordance with modern concepts, aqueous solutions of alkalis and acids in the human body is considered as an associated liquid, consisting of separate associated elements - neutral clusters and cluster ions of the general formula (H ₂ O) _n , [(H ₂ O) _n ] ⁺ , [( H ₂ O) _n ] ^- , [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ], etc. d, where the number of hydrogen-bonded water molecules can reach n times, according to some authors, under the action of HF and microwave energy, hundreds and even thousands of units. These effects respectively alter the electrical conductivity and biophotoluminescence of biological tissues. A change in the position of one structural element (water molecule) under the influence of any external factor or a change in the orientation of the surrounding neighboring water molecules in the cells provides high sensitivity of the entire information system of water to various external influences (electromagnetic, thermal, sound fields, bioinfluence, etc.). In addition, in water clusters, due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, the proton (H ⁺ ) can migrate according to the relay mechanism, leading to proton delocalization within the cluster, providing the release of singlet oxygen with a characteristic bright glow that kills cancer cells. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-informational, etc. In addition, aqueous solutions of various chemical elements are a source of superweak and weak alternating electromagnetic radiation. In this case, the induction of an external electromagnetic field can occur, causing resonant effects of the combination (superposition) of external electromagnetic fields with their own fields in biological objects with photowave radiation, capable of changing the structural and informational characteristics of biological objects, 80-90% consisting of water solutions with various chemical impurities and cause their photoluminescence.

Under the action of a high-frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, excitation, polarization and ionization of N ₂ , H ₂ , O ₂ and CO ₂ molecules occur. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of an object saturated with various chemical solutions, can color the glow crown in different colors. The shape of the glow crown, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body, granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells, release reactive forms of singlet oxygen contained in super axid radicals, hydrogen peroxide H ₂ O ₂ , and hydroxyl radical JOH in this case, weak chemilumination is observed, which increases multiple times in the presence of the sodium salt of the "Photosense" preparation and succinic acid. These effects are also multiplied by the action on blood vessels and cells of short-term electrical impulses, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the metachondria. This effect of electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nikola Tesla, when exposed to pulsed high-frequency energy of vessels with liquids that have the ability to emit light and luminescent lamps, which, without being connected to electrical wires, shone with bright light in the hands of Nikola Tesla, with which he also juggled, which caused genuine delight among the audience, while at that time inexplicable by nature, a phenomenon that only Nikola Tesla knew.

In biology, these factors are called collective luminescence stimuli that change the state of phagocytes in blood and tissues and their ability to increase the release of reactive oxygen species, and, accordingly, the protective functions of cells.

In cancer cells, aerobic respiration is absent in mitachondria and is replaced by oxygen-free glycolysis. Hydroxyaluminium of trisulfophthalocyanine Photosens, together with UC, when it enters the oncocell, inhibits glycolysis, but is not able to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely turn off glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or so that the hydroxyaluminium of trisulfophthalocyanine Photosens and YK prevails in the substrate over glucose. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In oncological cells, with an excess of hydroxyaluminium trisulfophthalocyanine Photosens and UC, and additional simultaneous exposure to HF and UHF electromagnetic hyperthermia on onco cells, oxidation processes are stimulated, which, when they are overabundant, have a toxic effect on onco cells.

It can be argued that the effect would be higher if the treatment was based on the treatment of hydroxyaluminium trisulfophthalocyanine Photosens and UC against the background of complete overlap of the intake of carbohydrates - glucose, as competitors of hydroxyaluminium trisulfophthalocyanine Photosens in onco cells. To do this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract are converted into glucose, which is extremely necessary for the nutrition of cancer cells. With such an introduction of oncocells into artificial glucose "starvation", then a person needs to inject high single doses of 0.05-0.8 mg / kg mass of hydroxyaluminium trisulfophthalocyanine of the Photosens preparation and 2400 mg of YaK. The required amount of drug intake, for HF and UHF treatment of tumors, should be taken for their maximum accumulation based on the maximum permissible one-time megadoses of Photosens hydroxyaluminium trisulfophthalocyanine, not exceeding 0.8 mg / kg and 3000 mg UC. Under the action of enzymes, in the human body, sodium hydroxyaluminiums of trisulfophthalocyanine Photosens and UC accumulate, and is determined by the intracellular concentration (the level of accumulation of the sensitizer) by its localization in the cell and photochemical activity (the quantum yield of the generation of singlet oxygen or free radicals), providing the fluorescent contrast to the tumor and an increase conductivity relative to the surrounding healthy biological tissues. When the hydroxyaluminium trisulfophthalocyanine Photosens and UC enter the tumor blood vessels, which have a large branched network with thin peripheral vessels and a low blood flow rate in them, the blood flow in these vessels of tumor tissues decreases even more when they are heated, which leads to blood coagulation in the tumor vessels. tissues, not allowing them to cool, due to the absence of a closed circulatory system. This is a direct cytotoxic effect on tumor cells, disrupting their blood supply, due to damage to the endothelium of the blood vessels of the tumor tissue, due to the hyperthermic effect and cytokine reactions, while macrophoges, leukocytes and lymphocytes are activated, leading to tumor necrosis. In the main human organs, rich in blood vessels, closed in the main circulatory system, there is a cooling of borderline healthy tissues exposed to HF and UHF hyperthermia. A "hungry" tumor is maximally saturated with Photosens' hydroxyaluminum trisulfophthalocyanine, 1.5-1.8 times higher and UC, 3-4 times higher than in normal healthy tissues, and accumulates by glycolysis transport in a sufficiently large amount on membranes and in interstitial fluid.

Hydroxyaluminium of trisulfophthalocyanine Photosens, and succinic acid is actively imported into endoplasmic reticules (EPR) (Endoplasmic reticulum, consisting of membranes and giving direction and active transport of substrates against gradients) of cells with the help of glucose transporters, including succinic acid. It should be noted that energy processes in cancer cells are transferred from the metachondria to the endoplasmic reticulum. It is here in the EPR that the UC and hydroxyaluminium of trisulfophthalocyanine of Photosens accumulate and the environment of the oncocell in this place differs significantly from ordinary cells, they are simply re-reduced here and here the oxidized UC is forced to release hydrogen, and together with the hydroxyaluminium of trisulfophthalocyanine Photosens, oxidize it to the hypoxyaluminum trisulfophthalocyanine Photosens. oxygen. From this moment on, the destructive action of YaK, hydroxyaluminium of trisulfophthalocyanine Photosens and HF and UHF energy begins on the oncocell. A "hungry" cancer cell at this time can repeatedly accumulate hydroxyaluminium trisulfophthalocyanine and succinic acid. perceives them on its membrane transporters for glucose. Since there are many times more glucose-consuming receptors in the oncolet than in healthy people, although the transport systems for the delivery of glucose, UC and Photosens hydroxyaluminium trisulfophthalocyanine into cells are common, this is the "Trojan horse" for oncocells. Thus, it is possible to very simply deceive the oncocells and inject into them hydroxyaluminium trisulfophthalocyanine Photosens, and succinic acid with the solution of the problem of supplying megadoses of hydroxyaluminium trisulfophthalocyanine Photosens and YK with subsequent processing of HF and microwave energy, then the phenomenon of death of oncocells will be manifold.

A "hungry" tumor in the absence of glycolysis is maximally saturated with hydroxyaluminum trisulfophthalocyanine of the Photosens preparation and succinic acid, 1.5-1.8 times and 3-4 times higher than in normal healthy tissues, stimulates the formation of macrophages and, T-lymphocytes under the action of the enzyme ferrachelatase, in sufficient large amounts on membranes and interstitial fluid. It is this chemical compound that is formed in the process of interaction of hydroxyaluminium trisulfophthalocyanine of the Photosens preparation and succinic acid in the internal environment of the body. Under the action of the oxidizing agent of lipoperoxide radicals and the formation of hydrogen, the significantly enhanced temperature action and additional action of the HF and UHF electromagnetic fields, active hydrogen radicals are formed, which then react with the superoxide radical, forming internal peroxide (dioxide), H ₂ O ₂ at HF and microwave hyperthermic decomposition, vitamin B ₉ yak. In this case, there is a multiple increase in the formation of excited oxygen molecules. The transition of molecules of sodium salt dioxide and lipid peroxides of succinic acid from the excited state to the ground state is accompanied by the emission of light quanta and a strong glow. As a result of these chemical reactions associated with a high release of reactive oxygen species and organic free radicals, cancer cells are burned out.

The method of "selective starvation" of superficial and deeply located onco cells in the human body, through the subsequent introduction or intake of various sensitizers, for selective maximum saturation of tumor cells with highly electrically conductive electron-ionic solutions of electrophotosensitizers with a maximum separation of the electrophysical properties of tumor and healthy tissues, followed by a selective effect on them electromagnetic fields of high frequency in combination with other methods - this is the most relevant scientific and practical direction in the fight against oncological diseases.

The study of the biophysical and biochemical mechanism of the complex effect of high-frequency and microwave energy on saturated onco cells and hydroxyaluminium of trisulfophthalocyanine Photosens and UC suggest three concepts of the death of oncocells, one assumes the importance of hydroxyaluminium trisulfophthalocyanine of the preparation Photosens, the other UC and hyperthermia of these tissues factors leads to a clear 100% death of cancer cells.

A number of researchers argue that the minimum concentration of hydroxyaluminium trisulfophthalocyanine Photosens, which is a prooxidant (lyco-oxidizing compounds that neutralize free radicals) and succinic acid, in the blood and tissues kill cancer cells without affecting healthy ones, due to the local oxidative stress-process of damage caused by oxidation, cellular DNA and depletion of adenosine triphosphate (ATP), the cell's energy source. Lipoperoxide with the formation of hydrogen among other accompanying molecules, an aggressive effect, causes a malfunction of the functioning of a certain enzyme responsible for "feeding" the cells of malignant tumors.

The "hungry" tumor is maximally saturated with the hydroxyaluminum trisulfophthalocyanine of Photosens and UC stimulates the formation of lipoperoxides with active release of hydrogen and significantly accelerates this process, under the action of HF and microwave heating, in a sufficiently large amount on the riticulum and in the interstitial interstitial fluid. It is these chemical compounds that are formed during the interaction of vitamin B ₉ and the internal environment of the body. A strong oxidizing agent of UC, together with the hydroxyaluminium trisulfophthalocyanine Photosens, is a factor or hormone that stimulates the mechanisms of self-destruction and death of cancer cells. Formation of sufficient doses of lipoperoxides with active release of hydrogen and hydroxyaluminium trisulfophthalocyanine Photosens around and inside oncocells and their apoptosis is possible only with a sufficiently large amount of succinic acid and Photosens. Under these conditions, the hydroxyaluminium of trisulfophthalocyanine Photosens and YK can act as an antioxidant or prooxidant, i.e. an oxidizing agent, including showing a destructive rather than a creative property of cancer cells. This is very important in the energy of cells. Therefore, the hydroxyaluminum trisulfophthalocyanine Photosens and UC can be designated as a switch of metabolism, which accelerates and optimizes aerobic, energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in mitachondria and is replaced by glycolysis. UC, upon entering the oncocell, inhibits glycolysis, but is unable to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely turn off glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or so that UC and hydroxyaluminium of trisulfophthalocyanine of Photosens prevail in the substrate over glucose and under the action of the electromagnetic field of HF and microwave, causing them to heat up, with a large release of lipoperoxides and active release of hydrogen with the formation of the main activators of death tumor cells. In healthy cells of UC and hydroxyaluminium trisulfophthalocyanine Photosens in small amounts in cytazole, it exhibits protective antioxidant properties. Hydroxyaluminium of trisulfophthalocyanine Photosens and UC are representative of intermediate acids that can accumulate in the cytosol of cells, and with further exposure to high-frequency and microwave energy disrupt the endothelium of the blood vessels of tumors and cytokine reactions that stimulate TNF-a, activating microphages, leukocytes and photoelectromocytes. energies damage tumor cells.

Tumor cells accumulate, in contrast to normal cells, a significant amount of homocysteine theolactone (HTL). Before homocysteine is inserted into a protein, it becomes a biological marriage, in the form (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone is practically not formed from it, but transformation into a cancer cell requires significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein that synthesizes the machine of the cancer cell works at full capacity, so it is more often mistaken. Tue found that interaction with organic acids (succinic acid), this substance forms the highly toxic 3-mercaptopropionic aldehyde (MPA). When succinic acid and hydroxyaluminium trisulfophthalocyanine of the Photosens preparation enter a cancer cell saturated with HTL, MRA is formed, which kills cancer cells. Destroying cancer cells, MPA eliminates the source of its formation, therefore, normal cells under the action of hydroxyaluminium trisulfophthalocyanine of the Photosens preparation, succinic acid and HF and microwave energies do not suffer from them much. and microwave energy in the blood, and in various organs of a person, a persistent therapeutic effect will be observed.

In healthy cells, hydroxyaluminium trisulfophthalocyanine of the preparations Photosens and YK entering the EPR do not change the biochemical metabolism in the cells, because pH and ORP (Oxidation-reduction potential) are not suitable for this, and the hydroxyaluminium of trisulfophthalocyanine of the Photosens preparation and YaK will be practically harmless for them and will be transformed on the glucose conveyor. In oncocells, the environment is different, re-reduced and hydroxyaluminium of trisulfophthalocyanine of the Photosens preparation and UC, to the maximum in the oncocell, everything is burned and destroyed at HF and SHF photoelectromagnetic hyperthermia due to lipid peroxidation (LPO). In this case, significant destruction occurs with the formation of toxic lipoperoxides that damage cell membranes, various organelles, mutation of nucleic acids, enzyme inoculation, destruction of nutrients and cell death. In this case, cell death proceeds not along the path of apoptosis, but along the path of powerful necrosis.

Hydroxyaluminium of trisulfophthalocyanine of the Photosens preparation and succinic acid in tumor cells is transformed, under the influence of temperature, with the formation of active forms of hydrogen and oxygen and other lipid peroxides. The more hydroxyaluminium trisulfophthalocyanine and UC in the oncocell, the more lipoperoxides and reactive forms of hydrogen and oxygen are formed in it, in comparison with healthy cells. An excess of lipid peroxides triggers the death of cancer cells. The process of death of cancer cells is initiated by HF and microwave fields by rapidly heating cancer cells to 49.5 ° C saturated with hydroxyaluminium trisulfophthalocyanine and UC and their rapid oxidative decomposition under the influence of temperature with a large release of lipoperoxides of reactive forms of hydrogen and oxygen, which is fatal for cancer cells.

This effect is proposed for the cure of cancer patients and is explained by the fact that at this time from 2 to 8 hours in normal cells of living biological objects after taking 0.05-0.8 mg / kg of weight of hydroxyaluminium trisulfophthalocyanine intravenously Photosens, and 2400 mg of succinic acid orally from 0.5-2.5 hours, they are quickly converted into bivalent heme of iron and lipoperoxide, under the action of the enzyme ferrochelatase, while maintaining a high contrast of the content of hydroxyaluminium trisulfophthalocyanine of the Photosens preparation, and UC in tumors, respectively, in 8-10 and 3-4 times higher than in healthy tissues, which significantly increases the electrical conductivity of tumors relative to the surrounding healthy biological tissues, reaching this difference many times.

With further simultaneous hyperthermia of tumor cells saturated with Photosense's hydroxyaluminum trisulfophthalocyanine and succinic acid for 195 seconds by wave radiation of HF and microwave fields, at the permitted frequencies of oscillations of the electromagnetic field f = 433 92 MHz, f = 915 MHz or 2450 MHz, with a heating rate of 0.066 ° С / sec until the final heating temperature of tumor cells is 49.5 ° С, tumor cells are destroyed.

This allows for simultaneous fluorescence diagnostics to clarify the border of tumors and simultaneous electromagnetic photodynamic hyperthermia of tumor cells with the energy of photowave radiation with the decomposition of hydroxyaluminium trisulfophthalocyanine of the Photosens preparation and YK in lipoperoxide with the release of active hydrogen and oxygen and hydroxides, which can effectively detect and even destroy undetectable tumor formations located in the deep layers of a biological object.

The main task for researchers is to enhance as much as possible the effect of maximum selective absorption of hydroxyaluminum trisulfophthalocyanine by cancer cells with simultaneous subsequent high-frequency irradiation of cancer cells in order to increase the effectiveness of treatment up to 100%. It has already been proven that such an effect is possible by the example of disinfection of biological objects from viral, fungal and bacterial infections with high-frequency and microwave energy. Numerous studies carried out by us at the Krasnoyarsk State Agrarian University and VIZR in St. Petersburg have confirmed 100% efficiency of disinfection of seeds of vegetable crops and living biological objects saturated highly conductive electronic-ionic solutions of microelements of high frequency and microwave energy against viral infections, which have a similar origin with onco cells.

A.S. No. 563938 USSR. Method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bul. No. 25. Certificate of the USSR No. 950214. Method of pre-sowing seed treatment / Tsuglenok N.V. - Registered in the registry on 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with high-frequency and microwave energy: guidelines / N.V. Zuglenok. - M .: Agropromizdat, 1989. Guidelines for the use of high-frequency and microwave energy in the preparation of seeds for sowing / N.V. Zuglenok. - M .: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfection of tomato seeds against viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute of Plant Protection (VIZR). - 1989. - T. 71. - S. 49-54. Method for disinfecting egg powder. Patent number: 1734632. Published: 23.05.1992 Authors: Tsuglenok N.V., Kolmakov Yu.V. IPC: А23в 5/02. Method of preparing a medium for diluting semen from the manufacturer Patent number: 1769422. Published: 27.06.1995. Authors: Tsuglenok, Ostashko, Shakhmatov, Silantyeva, Kontsedal.

The most important thing is that this method is harmless, does not have any special side effects for biological objects.

It has been proven that under the influence of carcinogens, oncoviruses are incorporated into a healthy cell and eventually dissolve in it, turning it into an oncocell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless; they simply are not. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the shallow depth of penetration of the electromagnetic wave of laser emitters does not allow burning deep-seated malignant tumors.

It is necessary to note another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein envelope filled with a mixture of nucleic acids and similarly tumor cells filled with solutions of intercellular fluid determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. HF and microwave heating in tumor tissues significantly increases the mobility of ions and electrons, increasing their electrical conductivity and dielectric losses, which further enhances their selective heating and apoptosis of tumor tissues.

The biophysical meaning of this method lies in the selective maximum saturation and accumulation of highly electrically conductive electron-ion solutions of electrophotosensitizers in tumor cells and in the maximum separation of the electrophysical properties of tumor and healthy tissues by the introduction of solutions of hydroxyaluminium trisulfophthalocyanine of the preparation Photosens and succinic acid and a significant increase in tumor cells. in the intercellular environment and on the walls of the reticulum. The reticulum is an electrical circuit where negative charges are evidently accumulated on one side of the membrane, and positive ones on the opposite side, therefore the reticulum is an electric transporter of glucose and other nutrients of cancer and healthy cells. Consequently, the reticulum is an electrical network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of the metachondria and the energetic operator structures on the outside of the cell - on the cilia. These proteins under certain situations in the environment of the cell, being discharged, can give an active signal to the reticulum and metachondria. This changes the balance that exists on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are triggered. One side of the reticulum membrane is connected to one type of entrance to the metachondria, and the opposite side to the exit from it. Thus, a single electric circuit is created for double active control of the metachondrial energy. The strength of the electric field on the reticulum keeps the metachondria under control. In this case, the metachondria pull in the charges accumulated on one side of the reticulum membrane and remove opposite charges to the other side of the reticulum membrane. The charges are thus not mixed and separated. This is important for ion exchange to take place in cells. Outwardly, the reticulum is similar to the capacitor plates, the more layers of plates, the greater its electrical capacity. Between the spacers there is a semiconductor saturated with solutions of hydroxyaluminium trisulfophthalocyanine Photosens and YaK. This capacitor, i.e. a powerful dense network of tumor membranes is very clearly visible through a microscope. In tumor cells, the number of membranes is much higher than in healthy ones. Accordingly, the density of tumor tissues and the capacity of the biological electrical capacitor are significantly higher than those of healthy tissues. When charging, negatively charged particles-electrons will be collected on one plate of such a capacitor, and ions, positively charged particles, will be collected on the other. Such a charged capacitor can turn into a current source if disconnected. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which is dumped on the metachondria, controlling their activity. The metachondria, in turn, are tuned so that they never allow the charges on the reticulums to drop below a critical level. In cancer cells, the charges inside the metahodria are sharply reduced and the entire regulation system is disrupted. This is the main control rod for the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not primary. As a result of the electrochemical energetics of the cell in the reticulum there is a circulation of substances, where the metachondria are the pump. With a lack of this cycle between the reticules and metachondria, due to electroosmosis, substances are sucked from the outside through the outer membrane and the gateways and sodium pump are opened on it. The medium on the membranes of the reticulum and the alkaline liquid substrate in tumor cells is re-reduced due to the excess of negative charges. This is what determines the chemical equilibrium in terms of pH of conjugated buffer chemical electroparic substances when the buffer system is discharged or restored. These processes are regulated by charges on the lining of the reticulum and metachondria. Chemical processes, in this case, are simply executors, intermediaries. The outer side of the metachondria provides a voltage dependent anion channel. This voltage maintenance mechanism is called VDAC and sets the conditions for the reticulum to work. It is here, on the outside of the membrane, that the Hexokinase II enzyme is located, which ensures the utilization of glucose or its substitutes. Separation, disconnection of the work of the outer mitochondrial membrane (VDAC) and Hexokinase II ensures the induction of apoptosis of tumor cells.

The metachondria works by pulling from the reticulum into itself as an electromagnetic pump, which requires power under high voltage. Without this effect, the highest tension of pulling in nutrients into the cell will not be possible. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Cancer cells, unlike normal cells, do not have cilia. This, the most damaging, energy level is absent in cancer cells. The only correct way to fight cancer cells is to find a weak spot in the energy of cancer cells and thereby destroy them. Metachondria set the degree of charge for ion pumps on the outer membrane of the cell and for starter structures that hold charges on the reticulum. These sensory structures can be most rapidly damaged and burned out, since the metachondria are the most efficient electrochemical furnaces. In the case of turning off the metachondria, the voltage gradient of the cell decreases sharply and the processes take place in onco cells over a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. The oncocell does not take on quality, since everything is concentrated on a small area of metachondria, but with a larger number, much more than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but at the same time the consumption of glucose will be much higher than in healthy tissues.

Membranes - the reticulums and the nuclei of the cell are the same, and the reticulum, as a capacitor, is contoured to the nucleus, with only one side of it, an electron, and dumps electrons into the nucleus. Thus, the charge in the reticulum also provides charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a super-powerful electrostatic charge inside the nucleus.

The main task for researchers remains to maximize the effect of maximum selective absorption of hydroxyaluminum trisulfophthalocyanine of the Photosens preparation and UC by cancer cells and increase the effectiveness of treatment by increasing the electrical conductivity of metachondria and reticulums of cancer cells with simultaneous selective HF and microwave hyperthermia of cancer cells. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. The electrical conductivity of cancer cells is due to the presence of mobile charged electrons in the reticulums and in the cell nucleus and ions in the mitachondria of the cell. The amount of electrical conductivity depends on the amount of electrical charges and their mobility. The electrical conductivity of living tissues is determined by the concentration of ions and their mobility, which is different in different tissues, and therefore, biological objects have the properties of conductors, semiconductors and dielectrics. The intercellular fluid saturated with solutions of hydroxyaluminum trisulfophthalocyanine Photosens and UC contains the maximum content of ions

The specific electrical conductivity of tumor tissues is high and amounts to more than 1 S ⋅ m-1. Large protein molecules have a lower electrical conductivity, up to 0.003 S ⋅ m-1. Intracellular membranes have a conductivity below (1-3⋅10 ^-5 ) S ⋅ m-1. The greatest values of electrical conductivity in the human body are found in liquid media (blood, lymph, bile, urine, cerebrospinal fluid and tumor cells (0.6-2.0 cm ⋅ m-1) and muscle tissue (0.2 cm ⋅ m- 1) Bone, adipose and nervous tissues, especially coarse-fibrous connective tissues and tissues of tooth enamel (10 ^-3 -10 ^-6 S ⋅ m-1) have the lowest electrical conductivity.

The electrical conductivity of cells and tissues at HF and UHF currents is much more complex. In this case, biological objects have both conductivity and capacitance, which characterizes the dielectric constant. The frequency dependence of electrical parameters and the absorption of energy of the electromagnetic field are determined by the size and shape of cells, the value of their permeability, the ratio between the volume of cells and intercellular spaces, the concentration of free ions in cells and the content of free water in them. All these factors lead to a change in the electrical conductivity of biological objects. A particularly significant factor for the metabolism of cancer cells is the content of glucose or its substitutes in them, in this case, the hydroxyaluminum trisulfophthalocyanine of Photosens and UC. If the human body has malignant tumors and metastases of stages 3 and 4, which actively and intensively assimilate glucose or its substitute - hydroxyaluminium trisulfophthalocyanine of the Photosens preparation and YK, they are converted into ATP in cancer cells much less than in healthy ones, as a result of which, cancer cells they get very hot and raise the temperature of the human body by 1-2 ° C. This physiological mechanism induces an increase in the temperature of tumor and adjacent normal tissues. The total rise in temperature, at present, is recorded by a microwave radiometer with an accuracy of 0.3 ° C, when determining the temperature of deeply located tumor and healthy tissues.

This process was partially studied by us when a biological object with tumor tissues was exposed to magnetic fields, which were subjected to daily complex action of a constant magnetic field with an intensity of 25 μT and an alternating magnetic field with a frequency of 3.1 Hz and an intensity of 5 μT, exposure for 60 minutes a day at a time. within 5 days. The proposed method of influencing constant and variable effects on ion exchange in mitochondria of cells and on negatively charged electrons on reticulums and cell nuclei made it possible to induce death of tumor cells using magnetotherapy, which by 40%, compared with control, freed biological objects from tumor cells ( Patent No. 2307681, authors: Tsuglenok N.V., Sergeeva E.Yu., Klimatskaya L.G. RU). Therefore, this direction of using magnetic and electromagnetic fields and their effect on the energy of tumor cells deserve special attention, confirmed by researchers from South Korea, who proposed using a powerful magnetic field to destroy tumor cells. In a powerful magnetic field, the tumor begins to kill itself.

A known method of destruction of cancer cells with microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the value of the resonant frequency is absorbed by the tumors. After that, a similar effect is carried out on healthy tissues bordering with the tumor, and the value of the resonance frequency of the absorption of these healthy tissues is determined. Simultaneously with hyperthermia, the values of resonance frequencies of energy absorption by tumors and healthy tissues are monitored, and when the values of resonance frequencies approach, energy absorption by tumors and healthy tissues is judged on the effectiveness of treatment. This method makes it possible to increase the efficiency of tumor treatment by the method of microwave hypothermia when they are heated to 43 ° C.

The main disadvantage of this method is the slight difference in heating of tumor and healthy tissues.

A known method for the destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6) the essence of the invention includes the introduction of ferromagnetic particles into the tumor localization area, followed by induction local heating, in the temperature range from 42 ° C to 45 ° C, during the time determined by the type of tumor, its size, localization and the type of ferromagnetic particles selected for induction heating, while heating is carried out only at the moments of decrease in tissue blood filling, i.e. at the moments of exhalation and diastole of the patient's heart. The heating range is controlled by a microwave deep thermometer, and heating is carried out automatically, with the help of a computer, in the biofeedback mode, according to the algorithms of the mathematical model of fluctuations in the thermal conductivity and heat capacity of the tissue, the hysteresis of heating and heat removal.

The main disadvantages of this method are the low localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in different spatial areas of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, Serdar Deger, Stefan Loening, Wolfgang Lanksch, Roland Felix. Journal of Magnetism and Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on thermolysis of magnetic nanoparticles introduced into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions through cell membranes, as well as generate inductive alternating electric fields that affect the operation of neural networks in the human body, associated with heating not only magnetic particles, but all cells. located in the area of injection of magnetic particles, and a strong spatial inhomogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

The known method of close-focus X-ray therapy with a total focal area of 100-120 Gy and remote gamma therapy for radiation destruction of malignant cells with a total focal area of 30-40 Gy (see Sh.Kh. Gantsev. Oncology, M .: Medical Information Agency. 2004, pp. 190-204; Stephen J., Withrow E., MacEwen G. Smal animal clinical oncology - 2001, p. 305-308).

However, this method, despite its prevalence, has the following disadvantages. In the treatment of some types of malignant neoplasms, for example melanoma, using gamma remote therapy, even in combination with immunotherapy, experience shows that it leads to 75-90% of tumor recurrence, and after 2-6 months metastases occur.

The known method of neuron - capturing selective destruction of melanoma (see V. N. Mitin, N. G. Kozlovskaya, AM Arnopolskaya Neuron-capture therapy of tumors of the oral cavity in dogs. All-Russian veterinary journal. 2006. No. 1, pp. 9-10) ...

The method involves intravenous injection of L-borphenylalanine into the blood, which selectively accumulates in a certain tumor - melanoma, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, there is a selective accumulation of L-borphenylalanine in melanoma cells. When a spatial zone comparable to a tumor containing L-borphenylalanine is irradiated by a beam of slow neurons obtained through a neuron guide from a nuclear reactor, melanoma cells are destroyed due to induced secondary local boron radiation.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced by using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to service.

3. Long-term irradiation of patients within an hour while monitoring the cardiovascular system.

4. Application of general anesthesia.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength coinciding with the absorption band of the photosensitizer (see Photodynamic therapy / Ed.TJ Dougherty / J. Clin. Laser Med Surg. 1996, Vol. 14, P219-348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of the accumulation of the photosensitizer in tumor cells compared to healthy cells, which is associated with a high density of blood vessels in the tumor compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, dye molecules pass into an excited electronic state, and upon collision with oxygen molecules dissolved in biological tissue, they transfer it from an unexcited to an excited electronic singlet state, with a typical lifetime of several microseconds. During this time, singlet oxygen molecules, having passed a characteristic path commensurate with the size of cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, the destruction of cells occurs only during exposure to laser radiation in the spatial area of laser irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. Photosensitizers used in practice - phthalationins, porphyrins, chlorins - have absorption bands of photosensitizers in the ultraviolet or visible region of the spectrum, and the lasers used cannot effectively penetrate to a depth not exceeding several millimeters. In addition, the photodynamic method has a low contrast in the accumulation of photosensitizers in cancer cells.

The closest to the claimed method is the destruction of biological tissue, which consists in introducing ethanol into it using a hollow game, characterized in that 95% ethanol is introduced in an amount equal to half the volume of biological tissue to be destroyed, then 5 ml of 20-30% ethanol is introduced, after which is carried out by heating with high-frequency current with the simultaneous introduction of 20-30% ethanol in an amount equal to the volume of biological tissue to be destroyed. The device contains a high-frequency current generator with two cylindrical electrodes located coaxially relative to each other, internal in the form of a hollow needle, through which ethanol is injected into the tumor (Abstract No. 2006113533 of the RF patent application). The disadvantages of this method include: the unreasonableness of selective absorption of ethanol by cancer and healthy cells, the difficulty of inserting a coaxial electrode into heterogeneous tumors, to organize uniform heating of tumor tissues not equally located from the exposed end of the needle.

The objective of the present invention is the local selective destruction of malignant tumors deeply located in human biological tissues, preselectively maximally saturated for 2-8 hours with hydroxyaluminium trisulfophthalocyanine Photosens 0.81 mg / kg body weight and UC 3000 mg orally for 0.5-2.5 hours irradiated, respectively , after 24 hours and 2.5 hours before the processing of photowave electromagnetic energy of HF and microwave to a temperature of 49.5 ° C with a heating rate of 0.066 ° C / sec. in accordance with the depth of penetration of the electromagnetic wave into biological tissues and with the depth of the location of tumor tissues in the human body at the permitted frequencies f = 13.56 MHz - 1100 cm, f = 27 MHz - 545 cm, f = 40.68 MHz - 370 cm , f = 433.92 MHz - 34.5 cm, f = 915 MHz - 16.5 cm and f = 2450 MHz - 6.1 cm.

The physical nature of microwave radiation is the physical field of moving electric charges in electric and magnetic fields, which are a single electromagnetic field (EMF), characterized by a frequency of oscillation f. The only difference is in the frequency with which electromagnetic oscillations occur with the corresponding wavelength. The biological effect of EMF on a living organism consists in the absorption of energy by biological tissues characterized by biophysical parameters - dielectric constant and conductivity.

The tissues of the human body, due to the high content of water in them, should be considered as dielectrics with losses. With general body irradiation, the EMF energy penetrates to a depth of 0.5 wavelengths. The intensity of exposure, exposure and dielectric loss and conductivity characterize the selective absorption of EMF by different tissues at the same density of EMF radiation.

where, λ is the wavelength;

c is the speed of propagation of the electromagnetic wave;

f is the frequency of oscillations of the electromagnetic field. The frequency with which the electromagnetic field fluctuates significantly affects the depth of penetration of the electromagnetic wave into a biological object. Table 1.

The reason lies in the commensurability with various physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, EMF wavelength λ = 33 cm, and at f = 2450 MHz, EMF wavelength λ = 12.2 cm. (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with the hydroxyaluminum trisulfophthalocyanine of the Photosens preparation and succinic acid are 1.5-1.8 and 3-4 times higher than its content in healthy tissues, respectively, its electrical conductivity differs so many times, i.e. the ability of tumor tissues to conduct an electric current is due to the presence of an acidic electrolyte in tumors, free charge carriers - electrically charged particles, which, under the influence of an external electric field in the thickness of the tumor, create a conduction current.

Another important parameter of dielectric and semiconductor materials, which tumors are, are dielectric losses, they serve to determine the electrical power spent on heating dielectrics and semiconductors in an electromagnetic field. In the reference literature, to characterize the ability of a dielectric to absorb the energy of an alternating electric field, the tgδ of the dielectric loss angle and the dielectric constant ε are used. The physical meaning of tgδ consists in the presence of dielectric losses leading to a phase shift between current and voltage, where the angle between them becomes less than 90 ° by the amount of quantitative losses of wave energy turn out to be proportional to the dielectric losses ε tgδ.

Conductivity losses in dielectrics with low specific volume resistivity, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with the changing wave electromagnetic field, converting electrical energy into heat. Tumor tissues are maximally saturated with hydroxyaluminium trisulfophthalocyanine Photosens and UC, in this case they are semiconductors containing several times more charged ions in comparison with the surrounding healthy tissues and, accordingly, their heating rate is many times higher than that of the surrounding healthy tissues at the same time. In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of polar water molecules in the direction of the lines of force of the electric field. Intramolecular friction arises, which once again increases the heating of tumor tissues.

The specific power of dielectric losses per unit volume of the dielectric is called dielectric losses, which can be calculated by the formula:

Ore = E ² fεtgδ, 10 ^-12 W / cm ³

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an electric field. To do this, it is necessary to know ε and tgδopyxol and healthy tissues, and thus very accurately calculate the heating rate to a predetermined heating temperature of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective absorption of hydroxyaluminium trisulfophthalocyanine of the Photosens preparation and UC by tumor tissues leads to their selective heating of tumors and electromagnetic photoluminescence to a higher temperature of 49.5 ° С when heating the surrounding healthy tissues to a temperature of 40 ° С during the same time, which leads to inoculation of tumor tissues and their subsequent destruction, which then, within a few days, are painlessly excreted by the body. The rate of heating by the wave energy of the electromagnetic field depends on the power of the dielectric generators and magnetrons.

With the oscillatory power of the electromagnetic field generators of 700-850 watts, 200-300 grams of tumor tissues can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors and their heating temperature is determined by the formula:

Where, Co is the heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ - heating temperature difference;

t - heating time, sec.

This formula allows you to select the required total specific power of Ore.ob. for high-frequency and microwave heating of tumor tissues Rud.op. up to a given difference in heating temperatures and the specific power of Rud.zd. released in healthy tissues, determined by the general formula:

Ore. vol. = Ore. op. + Ore zd.

Then the specific power in the irradiation region, taking into account the dielectric properties

и здоровых тканей

можно расчетным путем определить температуры их нагрева ΔT до необходимых заданных температур и определить время нагрева t и общую удельную мощность

облучаемой области (Таблица 2).Knowing the dielectric properties of tumor

and healthy tissues

it is possible by calculation to determine the temperature of their heating ΔT to the required specified temperatures and determine the heating time t and the total specific power

irradiated area (Table 2).

Similarly, knowing the dielectric parameters εtg δ and the specific density of tumor tissues in biological objects γ g / cm ³ , it is possible to calculate by calculation the specific power released in tumor tissues Rud op saturated with various electrophotosensitizers and determine the set temperature and calculate the time of their heating by HF and SHF energy, according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells with high-frequency and microwave-energy, intended for the local treatment of cancer patients with tumor tissues in various organs of the human body, characterized by the fact that before treatment a person is switched to a carbohydrate-free diet for 3 days to create glucose starvation and subsequent maximum saturation of cancer cells with an electron-ionic solution of hydroxyaluminum trisulfophthalocyanine intravenously in a megadose of 0.81 mg / kg of body weight of the Photosens preparation and oral administration of succinic acid in a megadose of 3000 mg, for their maximum accumulation in tumor tissues, respectively, in 1.5- 1.8 and 3-4 times more than in healthy ones 2-8 and 0.5-2.5 hours before physiotherapy, selective hyperthermia of tumor tissues with microwave energy is carried out in accordance with the depth of their location and the depth of penetration of the electromagnetic wave into the body human 6.1 centimeters, at the permitted frequency f = 2450 MHz, with a total heating rate of tumor tissues of 0.066 ° С / sec, for 195 sec until the heating temperature of tumor tissues is 49.5 ° С, when heating healthy tissues not higher than 40 ° С.