RU2723881C2 - Method for initiation of tumor cell death with ascorbic and succinic acids and hf- and uhf-energy of wave radiation - Google Patents

Abstract

FIELD: medicine; pharmaceuticals.SUBSTANCE: invention relates to a method for initiation of tumor cell death, intended for complex treatment of oncological patients having tumor tissues in all organs of human body, by hyperthermia HF- and UHF-energy, characterized by that person for 3 days is transferred to a non-carbohydrate diet to create glucose starvation and subsequent maximum saturation of oncological cells by electron-ionic solution of ascorbic acid in disposable megadose 350 mg, and after 4 hours with maximum accumulation of drug in tumor tissues is 2–4 times higher than in healthy tissues, selective hyperthermia of tumor tissues is performed with HF energy in accordance with depth of their location and penetration depth of electromagnetic wave into human body of 1100 cm, at the resolved frequency f=13.56 MHz, with a total heating rate of the tumor tissues of 0.017 °C/s for 360 s to the tumor tissue temperature of 43 °C, while heating the healthy tissues is not higher than 40 °C.EFFECT: said method results in thermal death of tumor tissues due to high dielectric tumor contrast and increasing the difference of its conductivity (dielectric losses) relative to surrounding healthy biological tissues, which reaches multiple values for various tumors.1 cl, 2 tbl

Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects of ascorbic and succinic acids and the energy of the wave RF and microwave radiation, known as RF and microwave hyperthermia.

In medicine, hyperthermia is called a significant increase in the human body temperature of more than 40 ° C. Hyperthermia for cancer treatment was used half a century ago. The German doctor von Ardenne opened a "thermal" clinic in a water bath for hopelessly oncologically ill patients, whom he heated to 42 ° C. After this procedure, no more than 17% of people survived, but they were completely cured. The rest were dying, not withstanding such a high temperature. This technology is now used in the United States, where the human body is heated to 42.5 ° C, followed by its return to life. This treatment technology can be effectively used for the selective heating of oncological tissues with RF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

A method for initiating the death of tumor cells by the electromagnetic energy of the wave RF and microwave radiation consists in the complex simultaneous exposure to ascorbic (AK) and succinic (UC) acids, and wave irradiation with electromagnetic energy of the RF and microwave tumor tissues. After taking a solution of ascorbic and succinic acids and, for their preservation in a significant amount in the tumor tissues of the human body, it takes time for them to accumulate in the tumor tissues within 0.5-4 hours. The maximum content of ascorbic and succinic acids in tumor tissues occurs precisely at this period of time and it is 2-6 times higher in tumor tissues than in healthy tissues due to selective absorption of AK and UC acid by tumor tissues.

The famous scientist Launus Pauling conducted scientific data on the positive effect of ascorbic acid (AK) on the slowdown of the cancer process. Some experts explained this by the fact that AK was taken by sick people in large doses in the form of tablets and did not always give a positive effect due to the fact that the concentration of AK in the tumor itself was low. It has been suggested that the tumor suppression mechanism is associated not with AK, but with its oxidized form. This was confirmed by scientific research. In experiments where Dehydro-AK was taken, the results were constantly renewed. These results were published in the 80s and early 90s of the XX century. The most complete description was given in 1991 in the journal of the American Association of Clinical Nutrition. Good results were obtained for leukemia in mice. The skepticism of the scientific world, however, did not allow to pay serious attention to these data. Lonely doctors continued research on the treatment of cancer with vitamin C, without delving into especially the theoretical foundations of this process. A researcher from Canada, John Tewe, tried to uncover the mechanisms of the effect of AK on tumor cells. His final work was published in Cancerletters in 2008. Currently, AK doctors use it as an additional tool that reduces the side effect of chemotherapy.

It is also recommended to treat cancer, succinic acid, which is effectively absorbed by the surfaces of tumor cells, reduce the hypoxia of tumor cells and saturate them with oxygen using succinic acid, which can penetrate tumor cells through cell membranes and can destroy the mitochondria of tumor cells and inhibit the growth of tumor tissue by transferring them to oxygen aerobic nutrition, without damage to normal cells. Succinic acid has a unique effect: it accumulates precisely in those areas that need it, ignoring healthy tissues. Succinic acid receptors do not have a noticeable toxic or side effect on animal or human cells, and they are selected from the group of these compounds consisting of succinic acid.

As the studies of the professor of the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences M.N. Kondrashova showed, the energy capacity of the ATP synthesis process during the oxidation of succinic acid is significantly higher than when oxidizing any other substrate. That is why many energy-dependent, that is, energy-consuming processes, for example, the accumulation of calcium ions and the provision of hydrogen biosynthesis, even in isolated mitochondria, can occur only during the oxidation of succinic acid. The works of the school of M.N.Kondrashova showed that in nature there exist and, if necessary, activate additional pathways for the formation of succinic acid. In particular, such an additional “injection” of succinic acid in a healthy person occurs during intensive work and during the recovery period after exertion, when the demand for rapid reproduction of ATP is especially high.

In hypoxia, the mitochondrial respiratory chain cannot accept hydrogen from any substrate other than succinic acid. After all, it is during its oxidation that hydrogen enters a region of the respiratory chain that is much closer to oxygen. At the same time, even with deep hypoxia, the ability to accept hydrogen is retained. In this case, the oxidation of succinic acid in mitochondria remains one of the few sources of ATP. The additional intake of succinic acid can significantly help the body.

The succinic acid that is formed in the mitochondria instantly burns in the same place, so the current steady-state concentration of succinic acid present in the tissues does not exceed 10-20 mg / kg of tissue mass at each time point and, as a rule, does not leave the mitochondria. Outside the mitochondria, outside the cell, it is practically absent in the bloodstream. It appears outside the mitochondria during severe anaerobiosis (complete lack of oxygen) or with deep hypoxia in some part of the tissue.

The receptor control systems of the body evaluate the appearance of succinic acid in the bloodstream as a signal that in some part of the body there are not enough energy resources, or there is oxygen starvation. Respectively. The body responds to this signal with shifts in the neuroendocrine. Hormonal regulation, improved peripheral blood flow, increased cardiac contractions, facilitated oxygen delivery by oxyhemoglobin and a number of other physiological and biochemical compensatory reactions.

Under conditions of oxygen deficiency, some of the cells die, while other cells adapt to such conditions and change. They make up for the lack of energy not due to oxygen, but due to the development of their internal activity. The respiratory failure leading to cancer is not so severe as to lead to cell death.

How amber works and why it works healthy is still an unknown question. Amber inhibits the growth of tumors, and a variety of them. This is fairly accurately established experimentally and does not contradict modern scientific views on the nature of cancer. Succinic acid prevents carcinogens and viruses from causing genetic malfunctions, leading to uncontrolled multiplication of cancer cells by normalizing energy metabolism in cells, thus delaying their division.

The proposed complex of dehydroascorbic acid obtained by the oxidation of AK of the ascorbic acid preparation and the dehydroacid acid of the succinic acid preparation is oxidized with hydrogen peroxide of the dehydroascorbic acid derivative (AA) in an alkaline medium, the reaction is catalyzed by iron heme, and causes chemiluminescence under the influence of high-frequency microwave and microwave radiation , with the active release of singlet oxygen. If an oxidizing agent, hydrogen peroxide, is added to an alkaline solution of cancer cells, then a glow occurs. In the presence of catalysts, this glow increases and becomes brighter. The role of catalysts for the solution of Dehydroascorbic and Dehydrojuccinic acids obtained as a result of oxidation of AA and UC is carried out by hemin of blood iron and various sodium compounds, succinates. These chemical activators of chemiluminescence enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. Observation with this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is Dehydroascorbic and Dehydrojuccinic acids, obtained as a result of oxidation of AK of the preparation "AK" in the presence of oxygen radicals. Under the action of AA oxidizing radicals of hydrogen peroxide, a formation occurs, which reacts with a superoxide radical to form internal peroxide (dioxide) of the UC, which leads to the formation of excited molecules. The transition of these molecules to the initial ground state is accompanied by emission by a quantum of light. Hydrogen peroxide AK is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, it is a relatively harmless compound, but in the presence of metal ions of variable valence of iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide H ₂ O ₂ , destructive hydroxyl is formed JOH radical, which causes mutations, and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl group of enzymes causes the activation of molecules, and actively enters into a chemical reaction with it when exposed to high-frequency energy, which leads to a bright glow of tumor tissues.

When placed in an alternating electromagnetic field of high tension and frequency of various biological bodies, they also begin to emit a characteristic radiance of various intensities and colors, from which one can judge the properties of the object under study. The method of “high-frequency photography” (Kirlian effect, kirlianography in honor of the inventor V.K. Kirlian) is now widely known in Russia and abroad as a method of experimental studies of electromagnetic fields and bioenergetic interactions. But the greatest scientific and practical interest is the study of the glow of biological objects in an alternating electromagnetic field of high frequency. explained by the photoelectromagnetic effect of photo-wave radiation and the luminescence of biological objects.

In accordance with modern concepts, aqueous solutions of alkalis and acids in the human body are considered as an associated liquid consisting of separate associated elements - neutral clusters and cluster ions of the general formula (H ₂ O) _n , [(H ₂ O) _n ] ⁺ , [( H ₂ O) _n ] ^- , [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ] etc. .d, where the number of water molecules bound into hydrogen bonds can reach n times, according to some authors, under the influence of HF and microwave energy, hundreds or even thousands of units. These effects respectively alter the electrical conductivity and biophotoluminescence of biological tissues. Changing the position of one structural element (water molecule) under the influence of any external factor or changing the orientation of surrounding neighboring water molecules in the cells provides high sensitivity of the entire water information system to various external influences (electromagnetic, thermal, sound fields, bio-exposure, etc.). In addition, in water clusters, due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, the proton (H ⁺ ) can migrate by the relay mechanism, leading to proton delocalization within the cluster providing singlet oxygen with a characteristic bright glow that kills cancer cells. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-information, etc. In addition, aqueous solutions, various chemical elements, is a source of super-weak and weak alternating electromagnetic radiation. In this case, the induction of an external electromagnetic field can occur, causing resonance effects of combining (superposition) of external electromagnetic fields with its own fields in biological objects under photo-wave radiation, which can change the structural and informational characteristics of biological objects, 80-90% of which are solutions of water with various chemical impurities and cause their photoluminescence.

Under the action of a high frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, the molecules N ₂ , H ₂ , O ₂ and CO ₂ are excited, polarized and ionized. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of an object saturated with various chemical solutions, can color the luminous corona in various color schemes. The shape of the glow corona, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells secrete active forms of singlet oxygen contained in super axide radicals, hydrogen peroxide H ₂ O ₂ , and hydroxyl radical JOH in this case, weak chemiluminescence is observed, which is amplified repeatedly in the presence of D-AK and AK during HF and microwave irradiation. These effects are also amplified many times by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the cell metachondria. This effect of the influence of electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nicolo Tesla, when exposed to pulsed high-frequency energy of vessels with liquids with the ability to emit light and fluorescent lamps that glowed without connecting to electric wires, with bright light in the hands of Nikola Tesla, with whom he also juggled, which caused genuine delight among the audience, while at the same time a phenomenon inexplicable by nature, which only Nicolo Tesla knew.

These factors in biology are called collective stimuli of luminescence that alter the state of blood and tissue phagocytes and their ability to increase the excretion of reactive oxygen species, and accordingly the protective functions of cells.

In cancer cells, aerobic respiration is absent in the mitochondria and is replaced by glycolysis. AK and UC upon entry into the cancer cell inhibits glycolysis and transfers it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely turn off glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or for AK and UC to prevail over glucose in the substrate. In healthy cells in any amount in cytazole, it exhibits protective antioxidant properties. In cancer cells, when there is an excess of it, it stimulates the processes of oxidation, due to their enrichment with divalent oxygen, which, when it is abundant, has a toxic effect on cancer cells.

It can be argued that there would be even less side effects, and the effect would be greater if treatment with powerful doses of AK and UC were based on the complete blocking of the intake of carbohydrates - glucose, as competitors for AK and UC in cancer cells. For this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract turn into glucose, which is essential for the nutrition of cancer cells. With this introduction of cancer cells into artificial glucose "starvation", then a person must enter high single doses of AK and UC with the addition of foods, fruit juices from unripe fruits and berries to the diet. When succinic acid enters the blood vessels of the tumor, attached to the main arteries and vessels of a person, having a large branched network with thin peripheral vessels and a low velocity of blood in them. The blood flow in these vessels of the tumor tissue decreases even more when they are heated due to its thickening, which, even more efficiently, leads to coagulation of blood in the vessels of the tumor tissue, not allowing them to cool, due to the lack of a closed circulatory system. In the main organs of man, rich in blood vessels closed into the main circulatory system, cooling of healthy border tissues subject to HF and microwave hyperthermia occurs. A “hungry” tumor is maximally saturated with ascorbic and succinic acid, which selectively accumulates in tumor tissues and 2-4 hours after AK administration and 2.5 hours after UC administration, respectively, 3-4 times more in tumor tissues than in normal healthy ones. Ascorbic and succinic acid stimulates the formation of hydrogen peroxide, in a large enough amount on membranes and interstitial fluid, enriched in divalent oxygen. It is this chemical compound that is formed during the interaction of ascorbic and succinic acid with the internal environment of the body. Ascorbic and succinic acid is a factor or hormone that stimulates the mechanisms of self-destruction and the degeneration of cancer cells. The formation of sufficient doses of ascorbic and succinic acid around and inside the cancer cells and their apoptosis is possible only with a sufficiently large amount of succinic acid intake. Under these conditions, ascorbic and succinic acid can manifest itself as an antioxidant or prooxidant, i.e. an oxidizing agent, including the destructive, rather than creative, property of cancer cells. This is very important in cell energy. Therefore, AK and UC can be described as a metabolic switch that accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in the mitochondria and is replaced by glycolysis. AK and UC upon entry into the cancer cell inhibits glycolis and transfers it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. In order to completely turn off glycolis in tumor cells, it is necessary to completely exclude glucose access or for AK and UC to prevail over glucose in the substrate. In healthy cells in any amount in cytazole, it exhibits protective antioxidant properties. In cancer cells, when there is an overabundance of it, it stimulates oxidation processes under the influence of HF and microwave energy, due to their enrichment with divalent oxygen, which, when it is oversupplied, has a toxic effect on cancer cells.

With the simultaneous entry of ascorbic and succinic acid into the blood vessels of the tumor, having a large branched network with thin peripheral vessels and a low speed of blood movement in them, with high-frequency and microwave heating it decreases even more at their high temperature of 44 ° C due to blood thickening, which, even more efficiently, leads to coagulation of blood in the vessels of the tumor tissues, not allowing them to cool, due to the lack of a closed circulatory system. In the main organs of man, rich in blood vessels closed into the main circulatory system, cooling of healthy border tissues subject to HF and microwave hyperthermia occurs. A “hungry” tumor is maximally saturated with ascorbic and succinic acids, several times higher than in normal healthy tissues, it stimulates the formation of hydrogen peroxide and ion salts of succinates, in a sufficiently large amount in the interstitial fluid. It is this chemical compound that is formed in the process of the interaction of ascorbic and succinic acid and the internal environment of the body. Peroxide and ionic salts of succinates with formed furmaric acid, is a factor or hormone that stimulates self-destruction mechanisms and the death of cancer cells. The formation of sufficient doses of hydrogen peroxide and other lipoperoxides around and inside the cancer cells and their apoptosis is possible only with a sufficiently large amount of ascorbic and succinic acids. Under these conditions, AK and UC can manifest themselves as an antioxidant or prooxidant, i.e. an oxidizing agent, including the destructive, rather than creative, property of cancer cells. This is very important in cell energy. Therefore, AK and UC can be described as a metabolic switch that accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in the mitochondria and is replaced by glycolysis. AK and UC upon entry into the cancer cell inhibits glycolis, but is not able to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. In order to completely turn off glycolis in tumor cells, it is necessary to completely exclude glucose access or for AK and UC to prevail over glucose in the substrate. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In cancer cells, when there is an overabundance of it, it stimulates the processes of oxidation, with the formation of hydrogen peroxide and lipoperoxides, which, when they are abundant, have a toxic effect on cancer cells.

Dehydroascorbic and succinic acids are actively imported into the endoplasmic reticules (EPR) (endoplasmic reticulum, consisting of membranes and setting the direction and active transport of substrates against gradients) of cells using glucose transporters. It should be noted that the energy processes in cancer cells are transferred from the mitochondria to the endoplasmic reticulum. It is here that D-AK and UC accumulate in the EPR and the onccell medium in this place is significantly different from ordinary cells. Succinic acid is simply reduced here to peroxidized lipids of the succinate ionic salts and furmaric acid, and here D-AK is obviously forced to recover to AK. From this moment, the destructive effect of AK and UC on the cancer cell begins. A “hungry” cancer cell at this time can repeatedly accumulate D-AK and UC, as perceives them on their membrane transporters for glucose. Since there are many times more glucose-consuming receptors in an onccell than healthy ones, although the transport systems for delivering glucose and D-AK and UC to the cell are common, this is the Trojan horse for onc cells. Thus, it is very easy to deceive oncocytes and pump D-AK and UC into them, with the solution of the problem of supplying megadoses of D-AK and UC, and then the phenomenon of death of oncocytes will be strengthened many times.

A “hungry” tumor is maximally saturated with D-AK, 2-6 times and UC 3-4 times higher than in normal healthy tissues, accumulates in a rather large amount on membranes and in interstitial fluid. A “hungry” tumor in the absence of glycolysis saturates for a maximum of 2-4 hours, and many times higher than in normal healthy tissues, it stimulates the formation of macrophages and T lymphocytes under the action of the ferrachelase enzyme, in a sufficiently large amount on membranes and interstitial fluid. It is this chemical compound that is formed during the interaction of AA and UC and hydrogen peroxide in the internal environment of the body. Under the action of the oxidizing agent of lipoperoxides and the formation of hydrogen, significantly enhanced temperature and the action and additional photodynamic action, electromagnetic fields of HF and microwave, active hydrogen and oxygen radicals are formed, which then reacts with superoxide radicals that accelerate and form internal peroxide (dioxide), H ₂ O ₂ with their hyperthermic decomposition, HF and microwave energy AK vitamin C and UK vitamin B. In this case, there is a multiple increase in the formation of excited oxygen molecules. The transition of AK and NQ molecules from the excited to the ground state is accompanied by the emission of light quanta and strong luminescence. As a result of these chemical reactions associated with a high release of active forms of hydrogen and oxygen and organic free radicals, a significantly enhanced temperature effect and the additional photodynamic action of electromagnetic fields of RF and microwave photo-wave radiation, cancer cells are burned.

The method of "selective starvation" of cancer cells superficial and deeply located in the human body, by subsequent administration or administration of various sensitizers, for the selective maximum saturation of tumor cells with highly conductive electron-ionic solutions of electrophotosensitizers with a maximum separation of electrophysical properties, tumor and healthy tissues, followed by selective effect on with electromagnetic fields of high frequency in combination with other methods - this is the most relevant scientific and practical direction in the fight against cancer.

The study of biophysical and biochemical mechanisms determines three concepts of the death of cancer cells, one assumes the importance of D-AK, the other D-UC, and the third RF and microwave photoelectrical hyperthermia, which, while using these methods, leads to a clear death of cancer cells. The main task for researchers remains to maximize the effect of the selective absorption of D-AK and AK by cancer cells with the subsequent subsequent high-frequency irradiation of cancer cells, with the aim of increasing the effectiveness of treatment to 100%.

It has already been proven that such an effect is possible by the example of disinfection of biological objects from viral, fungal and bacterial infections with HF and microwave energy. Numerous studies conducted by us in the Krasnoyarsk State Agrarian University and VIZR in St. Petersburg have confirmed the 100% effectiveness of disinfecting vegetable seeds and living bioobjects saturated highly conductive electron-ionic solutions of micronutrients of high frequency and microwave energy against viral infections having a similar origin with cancer cells.

A.S. No. 563938 of the USSR. A method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bull. Number 25. USSR Certificate No. 950214. The method of presowing treatment of seeds / Tsuglenok N.V. - Registered in the register 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with energy of high-frequency and microwave: methodical recommendations / N.V. Loam. - M .: Agropromizdat, 1989. Guidelines for the use of HF and microwave energy in the processes of preparing seeds for sowing / N.V. Loam. - M: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfecting tomato seeds against a viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute for Plant Protection (VIZR). - 1989.- T. 71. - S. 49-54. The method of disinfection of egg powder. Patent Number: 1734632. Published: May 23, 1992 Authors: Tsuglenok N.V. Kolmakov Yu.V. IPC: A23v 5/02. Method for preparing medium for diluting sperm of a manufacturer Patent number: 1769422. Published: June 27, 1995. Authors: Tsuglenok, Ostashko, Chess, Silantyev, Kontsedal.

It is proved that oncoviruses under the influence of carcinogens integrate into a healthy cell and dissolve in it over time, turning it into a cancer cell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless; they simply do not exist. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the small penetration depth of the electromagnetic wave of laser emitters does not allow to burn deep malignant tumors.

It is worth noting another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein shell filled with a mixture of nucleic acids and similarly tumor cells filled with intercellular fluid solutions determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. HF and microwave heating in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity and dielectric loss, which further enhances their selective heating and apoptosis of tumor tissues.

It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects.

A number of researchers argue that the minimal molar concentration of AA and UC, which are prooxidants (oxidative compounds that neutralize free radicals), kill cancer cells in the blood and tissues without affecting healthy cells, due to the local oxidative stress-damage process caused by oxidation of cellular DNA and depletion of adenosine triphosphate (ATP), a cell energy source. Hydrogen peroxide and other lipoperoxides, among other molecules accompanying it, aggressive action, causes a malfunction in the functioning of a certain enzyme responsible for the "nutrition" of cancer cells. AK is a representative of intermediate acids that can accumulate in the cytosol of cells.

Tumor cells accumulate, in contrast to normal ones, a significant amount of homocysteine teolactone (HTL). Prior to insertion of homocysteine into the protein, it becomes a biological defect in the form of (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone practically does not form from it, but conversion to a cancer cell requires significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein synthesizing the cancer cell machine works at full capacity, therefore it is often mistaken. Tue found that reacting with dehydroascorbic acid, this substance forms a highly toxic 3-mercaptopropionic aldehyde (MPA). When dehydroascorbic acid enters an HTL-saturated cancer cell, MPA forms, which kills the cancer cells. By destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer much from succinic acid. In this case, it can be argued that in the treatment of cancer with Dehydroascorbic and oxidized succinic acids obtained as a result of oxidation of AA and UC, both in the blood and in the human digestive system, a therapeutic effect is observed.

This healing effect is due to the fact that from 0.5 to 4 hours after the administration of living biological objects in normal cells, ascorbic and succinic acids quickly turn into dehydroascorbic and oxidized succinic acid and divalent iron heme under the action of the ferrochelatase enzyme. At the same time, it maintains a high contrast between the content of dehydroascorbic and oxidized succinic acid and its conversion to ascorbic and succinic acid lipoperoxides in the tumor, which significantly increases its electrical conductivity with a significant change in the dielectric properties of tumors relative to the surrounding healthy biological tissues, reaching this difference several times.

With further hyperthermia of tumor cells saturated with ascorbic and succinic acid, for 340 seconds, the wave radiation of the RF and microwave fields, with a permitted frequency of electromagnetic field oscillations f = 433 92 MHz, f = 915 MHz or 2450 MHz, with a heating rate of 0.021 ° C / sec for 340 sec. to a final temperature of heating of tumor cells 44 ° C. This allows simultaneous fluorescence diagnostics to clarify the boundaries of tumors and simultaneous photoelectromagnetic hyperthermia of tumor cells by the energy of photo-wave radiation and to identify and destroy, thus, even undetectable tumor formations located in the deep layers of a biological object.

The biophysical meaning of this method is to selectively saturate and accumulate highly electroconductive electron-ion solutions of electrophotosensitizers in tumor cells and to maximize the separation of electrophysical properties, tumor and healthy tissues and their saturation of AK and UC with a significant increase in the difference in electrical potentials of tumor and healthy cells in the intercellular medium and on the walls of the reticulum, for subsequent exposure to them with high-frequency energy. Reticulum is an electrical circuit where negative charges are evident on one side of the membrane and positive charges on the opposite side, which is why the reticulum is an electric transporter of glucose and other nutrients for cancerous and healthy cells. Therefore, the reticulum is an electric network charged with negative and positive charges. The balance of these charges is strictly controlled by mitochondrial activity and energy operator structures on the outside of the cell - on cilia. These proteins, in certain situations in the environment of the cell, discharging can give an active signal to the reticulum and mitochondria. This changes the balance of the existing charges on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are launched. One side of the reticulum membrane is connected to one type of entry into the mitochondria, and the opposite to the exit from it. Thus, a single electrical circuit is created for dual active energy control of mitochondria. The electric field strength on the reticulum keeps the mitochondria under control. In this case, mitochondria draw in the charges accumulated on one side of the reticulum membrane and remove the opposite charges on the other side of the reticulum membrane. The charges are thus not mixed and divided. This is important so that the cells undergo ion exchange. Outwardly, the reticulum is similar to the capacitor plates, the more layers of the plates, the greater its electrical intensity. Between the gaskets is a semiconductor saturated with Dehydroascorbic, succinic and ascorbic acids. This capacitor i.e. a powerful dense network of tumor membrane membranes is very clearly visible through a microscope. The number of membranes in tumor cells is much higher than in healthy ones. Accordingly, the density of the tumor tissue and the capacity of the biological electrical capacitor are significantly higher than healthy tissues. When charging such a capacitor on one plate, negatively charged electron particles will collect, and on the other - ions, positively charged particles. Such a charged capacitor can turn into a current source if it is turned off. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which discharges the charge on the mitochondria, controlling their activity. Mitochondria, in turn, are tuned in such a way that they never allow reticulum charges to drop below a critical level. In oncological cells, the charges inside the mitochondria sharply decrease and the entire regulatory system is disrupted. This is the main core of the control of the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not basic. As a result of the electrochemical energy of the cell in the reticulum, there is a cycle of substances where mitochondria are the pump. With the lack of this cycle between the reticuli and mitochondria due to electroosmosis, there is a suction of substances from the outside through the outer membrane and the opening of locks and a sodium pump on it. The medium on the membranes of the reticulum and the liquid substrate is re-restored, due to the excess of minus charges. This determines the chemical equilibrium in pH of conjugated buffer electro-chemical substances when the buffer system is discharged or restored. Charges on the plates of the reticulum and mitochondria regulate these processes. Chemical processes, in this case, are simply performers, intermediaries. The outer side of the mitochondria provides the voltage of the dependent anion channel. This voltage maintenance mechanism, called VDAC, sets the conditions for the reticulum to work. It is here that on the outer side of the membrane is the enzyme Hexokinase II, which ensures the utilization of glucose. Maximum separation, disruption of the outer mitochondrial membrane (VDAC) and Hexokinase II provides the induction of apoptosis of cancer cells.

Mitochondria works by pulling from the reticulum into itself as an electromagnetic pump, the necessary power under high voltage. Without this effect of the highest voltage, there will be no pulling in nutrients into the cell. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Oncocytes, unlike normal cells, do not have cilia. This, the most striking, energy level in cancer cells is absent. The only right way is to maximize the separation of the properties of tumor and healthy cells, and to find weaknesses in the energy of cancer cells and thereby destroy them. Mitochondria determine the degree of charge to ion pumps on the outer membrane of the cell and starter structures that hold charges on the reticulum. These sensory structures can most quickly be damaged and burn out, since mitochondria are the most effective electrochemical furnaces. In the event of mitochondrial shutdown, the cell voltage gradient decreases sharply and the processes go on in the cells on a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. A cancer cell does not take quality, since everything is concentrated on a small area of mitochondria, but with a larger number, much larger than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but at the same time, glucose consumption will be much larger than in healthy tissues.

The membranes of the reticulum and the nucleus of the cell are the same; moreover, the reticulum, as a capacitor, is contoured to the nucleus by only one of its electron sides and dumps electrons into the nucleus. Thus, the reticulum charge also provides a charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a heavy-duty electrostatic charge inside the nucleus.

In healthy cells, D-AK and UC, entering the EPR will not be restored to AK and UC, because pH and redox potential (redox potential) are not suitable for this, and D-AK and UC for them will be practically harmless and transform on the glucose conveyor. In cancer cells, the environment is different, converted to ascorbic and succinic acids, which try to burn and destroy everything in the cancer cell to the maximum due to lipid peroxidation (LPO). In this case, significant damage occurs with the formation of toxic lipid peroxide, damage to cell membranes, various organs, mutation of nucleic acids, inoculation of enzymes, destruction of nutrients and cell death. In this case, cell death does not follow the path of apoptosis, but overt necrosis.

During high-frequency and microwave heating, ascorbic and succinic acid in cancer cells is converted to dehydroascorbic and oxidized succinic acid, which is an ionic solution of salts of succinates and fumaric acid, which under the influence of high temperature 44 ° C, for 340 sec, forms hydrogen peroxides and other lipid peroxides. The more dehydroascorbic and succinic acids in the cancer cell, the more succinates and hydrogen peroxide are formed, in comparison with healthy cells. Excess hydrogen peroxide, and succinates and fuarmaric acid triggers the mechanism of death of cancer cells. The death of cancer cells is initiated by the HF and microwave field by rapidly heating the cancer cells to 44 ° C, due to AK, YAK and D-AK of their rapid oxidative decomposition under the influence of temperature with a high release of hydrogen peroxide and the form of succinates and fuarmaric acid, which is detrimental to cancer cells .

The most complete accumulation of ascorbic and succinic acids in the tumor occurs within 0.5-4 hours after ingestion. Then the level of ascorbic and succinic acid in the tumor gradually decreases, reaching its initial values 24 hours after taking the drugs. For treatment, the cancer patient needs to take one-time megadoses to maximize the accumulation in the tumor tissue of succinic up to 3000 mg and ascorbic acid up to 500 mg orally for 4 hours and 2.5 hours before the HF and microwave hyperthermia of the tumor tissue with wave radiation energy, with a heating rate 0.021 ° C / s to a temperature of 44 ° C.

As a result of this process, the tumors denature and, as a result, the decay products of the tumor cells are excreted by the body, within 2-4 weeks, independently, naturally, using further intake of succinic acid, excluding surgical intervention in the human body. An overdose of succinic acid was not observed. With a slight excess of the required amount of the drug, an excess of succinic acid is excreted without causing any harm to healthy body tissues.

The main task for researchers remains to maximize the effect of maximum selective absorption of ascorbic and succinic acids by cancer cells and increase the effectiveness of treatment by increasing the electrical conductivity of mitochondria and reticulums of cancer cells. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. The electrical conductivity of cancer cells is due to the presence of mobile charged electrons in them on the reticulums and in the cell nucleus and ions in the mitochondria of the cell. The value of electrical conductivity depends on the number of electric charges and their mobility. The electrical conductivity of living tissues is determined by the concentration of ions and their mobility, which is different in different tissues, and therefore biological objects have the properties of conductors, semiconductors and dielectrics. The intercellular fluid saturated with ascorbic and succinic acids contains the maximum ion content and the electrical conductivity of the tumor tissue is high and is more than 1 S болееm-1. Large protein molecules have a lower electrical conductivity, up to 0.003 S⋅m-1. Intracellular membranes have a conductivity below (1-3⋅10 ^-5 ) cm⋅m-1. The highest values of electrical conductivity in the human body are in liquid media (blood, lymph, bile, urine, cerebrospinal fluid and tumor tissue (0.6-2.0 S⋅m-1) and muscle tissue (0.2 S⋅m- 1) .Bone, adipose, and nerve tissues have the lowest electrical conductivity, especially coarse-fibrous connective tissue and tooth enamel tissue (10 ^-3 -10 ^-6 S cm-1). The conductivity of cells and tissues in RF is much more complex. and microwave currents.In this case, biological objects have conductivity and capacitance, which characterizes the dielectric constant.The frequency dependence of the electrical parameters and the absorption of electromagnetic energy are determined by the size and shape of the cells, their permeability, the ratio between the volume of cells and intercellular spaces, the concentration of free ions in cells and the content of free water in them.All of these factors lead to a change in the electrical conductivity of biological objects. Another factor for the metabolism of cancer cells is the content of glucose or its substitutes, in this case ascorbic and succinic acids. If there are malignant tumors and metastases in the human body that actively and intensively metabolize glucose or its substitute ascorbic and succinic acids, they are converted to ATP in cancer cells significantly, lower than in healthy ones, as a result of which, cancer cells become very warm and increase human body temperature 1-2 ° C. This physiological mechanism induces an increase in the temperature of the tumor and surrounding normal tissues. The total temperature increase, at present, is recorded by a microwave radiometer, which allows accurate 0.3 ° C, to control the temperature of deeply located tumors and healthy tissues in the human body.

This process was partially studied by us when exposed to a biological object with tumor tissues, which were subjected to complex daily exposure to a constant magnetic field with an intensity of 25 μT and an alternating magnetic field with a frequency of 3.1 Hz and an intensity of 5 μT, exposure time of 60 minutes per day at a time 5 days. The proposed method for the impact of constant and variable effects on ion exchange in the mitochondria of cells and on negatively charged electrons on the reticulums and cell nuclei allowed the induction of death of tumor cells using magnetotherapy, which 40%, compared with the control, freed biological objects from tumor cells ( patent No. 2307681, authors: Tsuglenok N.V., Sergeeva E.Yu., Klimatskaya L.G. RU). Therefore, this direction of using magnetic and electromagnetic fields and their impact on the energy of tumor cells deserve special attention, confirmed by researchers from South Korea, who proposed using a powerful magnetic field to destroy tumor cells. In a powerful magnetic field, the tumor begins to kill itself.

It is necessary to note another very important biophysical process - an increase in the electrical conductivity of tumor tissues, determined by a significant concentration of ions and electrons and their mobility. With increasing temperature in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity.

A known method of destruction of cancer cells during microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the resonance frequency of the absorption of the tumors is detected. Then they carry out a similar effect on healthy tissues bordering on the tumor and reveal the value of the resonance frequency of absorption of these healthy tissues. Simultaneously with hyperthermia, the values of the resonant frequencies are monitored by the absorption of energy by tumors and healthy tissues, and when the values of the resonant frequencies come closer together, the absorption of energy by tumors and healthy tissues is judged on the effectiveness of the treatment. This method allows to increase the effectiveness of the treatment of tumors by microwave hypothermia when they are heated to 43 ° C.

The main disadvantage of this method is the small difference in the heating of tumor and healthy tissues.

A known method of destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6) the essence of the invention includes the introduction into the tumor localization region of ferromagnetic particles, followed by local induction heating, in the temperature range from 42 ° C to 45 ° C, over time, determined by the type of tumor, its size, localization and type of ferromagnetic particles selected for induction heating, while heating is carried out only at times when the blood supply to the tissue decreases, i.e. at the time of exhalation and diastope of the patient’s heart. The heating range is controlled by a microwave depth thermometer, and heating is carried out automatically, using a computer, in biocontrol mode, according to the algorithms of a mathematical model of fluctuations in the thermal conductivity and heat capacity of the fabric, hysteresis of heating and heat removal.

The main disadvantages of this method is the small localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in various spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, Serdar Deger, Stefan Loening, Wolfgang Lanksch, Roland Felix Journal of Magnetis mand Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on the thermolysis of magnetic nanoparticles introduced into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions across cell membranes, as well as induce inducing alternating electric fields that affect the functioning of neural networks in the human body, associated with heating not only magnetic particles, but also all cells, located in the area of introduction of magnetic particles, and a strong spatial heterogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

A known method of short-focus x-ray therapy with a total focal zone of 100-120 Gy and remote gamma therapy for radiation destruction of malignant cells with a total focal zone of 30-40 Gy (see Sh. H. Gantsev. Oncology, M.: Medical Information Agency. 2004, p. 190-204; Stephen J., Withrow E., MacEwen G. Smalanimalclinicaloncology - 2001, p.305-308).

However, this method, despite the prevalence, has the following disadvantages. In the treatment of certain types of malignant neoplasms, for example melanoma, with the help of remote gamma therapy, even in combination with immunotherapy, as experience shows, leads to a 75-90% tumor recurrence, and metastases occur after 2-6 months.

The known method of neuron - capture selective destruction of melanoma (see VN Mitin, NG Kozlovskaya, AM Arnopolskaya Neuron-capture therapy of oral tumors in dogs. All-Russian Veterinary Journal. 2006. No. 1, pp. 9-10) .

The method involves intravenous administration of L-borphenylalanine, which selectively accumulates in a particular melanoma tumor, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, selective accumulation of L-borphenylalanine in melanoma cells occurs. Upon irradiation of a spatial zone commensurate with a tumor containing L-borphenylalanine by a beam of slow neurons obtained from a neuron guide from a nuclear reactor, melanoma cells are destroyed due to the induced secondary local radiation of boron.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced when using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to serve.

3. Long-term patient exposure for an hour when monitoring the cardiovascular system.

4. The use of general anesthesia.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength that coincides with the absorption band of the photosensitizer (see Photodynamictherapy / Ed. TJ Dougherty / J. Clin. LaserMedSurg. 1996, Vol. 14, P 219-, p. 348; RF patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of photosensitizer accumulation in tumor cells compared to healthy cells, which is associated with a higher density of blood vessels in the tumor compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, the dye molecules transform into an excited electronic state and, when they collide with oxygen molecules dissolved in biological tissues, transfer it from an unexcited to an excited electronic singlet state with a typical lifetime of several microseconds. During this time, the molecules of singlet oxygen, having passed a characteristic path commensurate with the size of the cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, cell destruction occurs only during exposure to laser radiation in the spatial region of laser beam irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. Used in practice, photosensitizers-phthalacins, porphyrins, chlorins have absorption bands in the ultraviolet or visible spectral range of the photosensitizers, and the lasers used cannot effectively penetrate to a depth of not more than a few millimeters. In addition, the photodynamic method has a low contrast accumulation of photosensitizers in cancer cells.

Closest to the claimed method is the destruction of biological tissue, which consists in introducing ethanol into it using hollow play, characterized in that 95% ethanol is introduced in an amount equal to half the volume of biological tissue to be destroyed, then 5 ml of 20-30% ethanol are introduced, after which is carried out by heating with a high-frequency current with the simultaneous introduction of 20-30% ethanol in an amount equal to the volume of biological tissue to be destroyed. The device contains a high-frequency current generator with two cylindrical electrodes located coaxially relative to each other, internal in the form of a hollow needle through which ethanol is introduced into the tumor (Abstract No. 2006113533 of the patent of the Russian Federation). The disadvantage of this method can be attributed to: the unreasonableness of the selective absorption of ethanol by cancerous and healthy cells, the difficulty of introducing a coaxial electrode into heterogeneous tumors, for organizing uniform heating of tumor tissues that are not equally located from the bare end of the needle.

The objective of the present invention is the local selective destruction of malignant tumors deeply located in human biological tissues, pre-selectively maximally saturated for 4 hours with ascorbic and succinic acids, respectively, with simultaneous selective HF and microwave heating of the tumors, and with secondary reverse heating, after turning off the HF and Microwave energy supply from tumor tissues heated to a temperature of 44 ° C HF and microwave energy with a heating rate of 0.021 ° C / s, from the boundary layers of the tumor to healthy tissues due to heat contact from high temperature from the tumor tissue, with minimal destruction of the surrounding healthy biological tissue cells.

According to studies on RF and microwave hyperthermia of tumor tissues that are maximally saturated with AK and UC at a temperature of 44 ° C, the border between the necrosis zone and healthy tissue is several cells. The destruction zone of tumor tissue includes a small periphery of normal healthy tissues, which excludes the regenerating cells from metastasis by their secondary necrosis from tumor tissues.

The physical nature of microwave radiation is the physical field of moving electric charges in electric and magnetic fields, which are a single electromagnetic field (EMF), characterized by an oscillation frequency f. The difference is only in the frequency with which electromagnetic oscillations of the corresponding wavelength occur. The biological effect of EMF on a living organism consists in the absorption of energy by biological tissues, characterized by biophysical parameters - dielectric constant and conductivity.

The tissues of the human body, due to the high content of water in them, should be considered as lossy dielectrics. With general body irradiation, EMF energy penetrates to a depth of 0.5 wavelength. The intensity of exposure, exposure and dielectric loss, and conductivity characterize the selective absorption of EMF by various tissues at the same EMF radiation density.

where, λ is the wavelength;

c is the propagation velocity of the electromagnetic wave;

f is the frequency of the electromagnetic field.

The frequency with which oscillations of the electromagnetic field occur significantly affects the depth of penetration of an electromagnetic wave into a biological object.

The reason is commensurability with various physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, the wavelength of the EMF λ = 33 cm, and at f = 2450 MHz, the wavelength of the EMF λ = 12.2 cm (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with ascorbic and succinic acid are 8-10 times higher than its content in healthy tissues, respectively, its electrical conductivity is so many times different, i.e. the ability of tumor tissues to conduct an electric current is due to the presence in the tumors of an acid electrolyte, free charge carriers — electrically charged particles, which, under the influence of an external electric field in the thickness of the tumor, create a conduction current.

Another important parameter of dielectric and semiconductor materials such as tumors are dielectric losses. They serve to determine the electric power spent on heating dielectrics and semiconductors in an electromagnetic field. In the reference literature, for the characteristics of the ability of a dielectric to absorb the energy of an alternating electric field, it uses tanδ of the dielectric loss angle and permittivity ε. The physical meaning of tg is the presence of dielectric losses leading to a phase shift between the current and voltage where the angle between them becomes less than 90 ° by an amount, the quantitative losses of wave energy are proportional to the dielectric losses ε, tanδ.

Losses on electrical conductivity in dielectrics having a low specific volume resistance, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with a changing wave electromagnetic field, converting electrical energy into heat. Tumor tissues are maximally saturated with AK and UC, in this case they are semiconductors containing several times more charged ions in comparison with surrounding healthy tissues and, accordingly, their heating rate is many times higher than that of surrounding healthy tissues at the same time. In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of the polar water molecules in the direction of the electric field lines. Intramolecular friction occurs, which once again enhances the heating of tumor tissues.

The specific power of the dielectric loss, referred to the unit volume of the dielectric is called the dielectric loss, which can be calculated by the formula.

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an electric field. For this, it is necessary to know ε and tanδ of tumor and healthy tissues, and thus very accurately calculate the heating rate to a given temperature of heating of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective absorption of AK and UC by tumor tissues leads to their selective heating of tumors and electromagnetic photoluminescence to a higher temperature of 50 ° C upon heating, at the same time, of healthy tissues surrounding them to a temperature of 40 ° C, which leads to the inactivation of tumor tissues and their subsequent destruction , which then, within a few days, are painlessly excreted by the body. The rate of heating by the wave energy of an electromagnetic field depends on the power of dielectric generators and magnetrons.

, выделяемую в биологическом объекте с учетом ε и tgδ опухолевых и здоровых тканей, можно очень точно рассчитать скорость нагрева до заданной температуры нагрева опухолевых и окружающих здоровых тканей в однородном электромагнитном поле (ЭМП). по формуле:Knowing specific power

, allocated in a biological object, taking into account ε and tanδ of tumor and healthy tissues, it is possible to very accurately calculate the heating rate to a given temperature of heating of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF). according to the formula:

With an oscillatory power of electromagnetic field generators of 700-850 watts, 200-300 grams of tumor tissue can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors, and their heating temperature is determined by the formula:

Where, Co - heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ is the temperature difference of the heating;

t is the heating time, sec.

для ВЧ и СВЧ нагрева опухолевых тканей

до заданной разницы температур нагрева и удельную мощность

выделяемую в здоровых тканях определяемую по общей формуле:This formula allows you to select the required total specific power

for rf and microwave heating of tumor tissues

up to a given temperature difference of heating and specific power

secreted in healthy tissues determined by the general formula:

Then the specific power in the irradiation region, taking into account the dielectric properties:

и здоровых тканей

, можно расчетным путем определить температуры их нагрева ΔT до необходимых заданных температур и определить время нагрева t и общую удельную мощность

, облучаемой области.Knowing the dielectric properties of the tumor

and healthy tissues

, it is possible by calculation to determine the temperature of their heating ΔT to the required set temperatures and determine the heating time t and the total specific power

irradiated area.

, и определить заданную температуру и рассчитать время их нагрева ВЧ и СВЧ энергией, по выше приведенным формулам.Similarly, knowing the dielectric parameters εtgδ and the specific density of tumor tissues of saturated electrophotosensitizers in biological objects γ g / cm ³ , it is possible to calculate the specific power released in tumor tissues by calculation

, and determine the set temperature and calculate the time of their heating by high-frequency and microwave energy, according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells, intended for the complex treatment of cancer patients having tumor tissues in various organs of the human body by hyperthermia of the RF and microwave energy, characterized in that the person is transferred to a carbohydrate-free diet for 3 days to create glucose starvation and subsequent maximum saturation of cancer cells with electron-ionic solutions of ascorbic and succinic acid, respectively, in megadoses of 500 mg in 4 hours and 3000 mg in 2.5 hours by oral administration before treatment, with their accumulation in tumors 2-6 times higher than in healthy tissues, selective hyperthermia of the tumor tissue with RF energy is carried out in accordance with the depth of their location and the depth of penetration of the electromagnetic wave into the human body 1100 centimeters, at a permitted frequency f = 13.56 3 MHz, with a total heating rate of the tumor tissue of 0.021 ° C / s, for 340 sec. To the temperature of the tumor tissue 44 ° С, when healthy tissues are heated no higher than 40 ° С.