RU2723884C2 - Method of initiating the death of tumor cells with sodium chloride chlorine-e6, succinic acid and hf and shf wave radiation energy - Google Patents

Abstract

FIELD: medicine.SUBSTANCE: invention is intended for inducing death of tumor cells in living biological objects of sodium salt "Chlorine-e" contained in medical preparation Chlorin-e, succinic acid (SC) and energy of HF and SHF radiation, known as HF and SHF hyperthermia. Described is a method for initiation of tumor cell death, intended for complex treatment of oncological patients, having tumor tissues with metastases for their hyperthermia in human organs, characterized by that, prior to treatment, a person is transferred to a no-carbohydrate diet for 3 days to produce glucose fasting of oncological cells and maximum saturation of oncological cells by electron-ion solutions, with subsequent administration to a human orally sodium chloride "Chlorine-e" in megadoses of 120 mg per person for 3–5 hours and succinic acid orally in megadoza 2500 mg for 0.5–2.5 hours before treatment with maximum accumulation of drugs in tumor tissues is 3–4 times higher than in healthy tissues, selective hyperthermia of tumor tissues HF energy in accordance with the depth of their location and penetration depth of the electromagnetic wave in the human body =1100 cm, at the allowed frequency f=13.56 MHz, with total heating rate of tumor tissues at these frequencies of 0.062 °C/s, for 198 s to heating temperature of tumor tissues 49 °C, while heating healthy tissues of not more than 40 °C.EFFECT: technical result is thermal death of tumor tissues due to high dielectric contrast of tumor and increasing difference in its conductivity (dielectric loss) relative to surrounding healthy biological tissues, reaching multiple values for various tumors; this allows for fluorescence diagnostics to specify boundaries of tumors and to conduct simultaneous hyperthermia of tumor cells of electromagnetic HF and SHF energy of photo-wave radiation and detect and destroy, thus, even undetectable tumor formations located in deep layers of biological object.1 cl, 2 tbl

Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects of the sodium salt "Chlorin- ₆ " contained in the medicinal product Chlorin- ₆ , succinic acid (UC) and the energy of the wave RF and microwave radiation, known as RF and Microwave hyperthermia.

Known methods for initiating the death of tumor cells of HF and microwave hyperthermia. Hyperthermia is called in medicine as a significant increase in human body temperature over 40 ° C. Hyperthermia for cancer treatment was used half a century ago. The German doctor von Ardenne opened a "thermal" clinic in a water bath for hopelessly oncologically ill patients, whom he heated to 42 ° C. After this procedure, no more than 17% of people survived, but they were completely cured. The rest were dying, not withstanding such a high temperature. This technology is now used in the United States, where the human body is heated to 42.5 ° C, followed by its return to life. This treatment technology can be effectively used for the selective heating of oncological tissues with RF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

The method of initiating the death of tumor cells by electromagnetic energy of wave radiation consists in the complex simultaneous exposure to sodium salts of Chlorin-e ₆ , succinic acid (UC) and the energy of the wave RF and microwave radiation, which include a concentrate for preparing a solution for infusion of the drug Chlorin-e ₆ . The drug Chlorin-e ₆ , and UC is very soluble in aqueous solutions. After intravenous administration of a solution of Chlorin-e _6, it takes time for its maximum accumulation in tumor tissues within 3-5 hours and succinic acid for 0.5-2.5 hours to accumulate and maintain a significant amount in the tumor tissues of the human body. The maximum content of Chlorin-e ₆ sodium salt and UC in tumor tissues occurs precisely at this time period, and it is 3-4 times higher than in healthy tissues due to the selective absorption of these drugs by tumor tissues. The drug Chlorin-e ₆ is excreted and redistributed from normal tissues and the circulatory system of a person to the tumor a day later, by 94% after intravenous administration, and succinic acid is excreted after 2.5 after administration. At the same time, during this period, HF and SHF, selective hyperthermia of tumor cells for 198 sec by wave radiation, with a heating rate of 0.062 ° C / sec to a final temperature of heating of tumor cells of 49 ° C at an allowed frequency of electromagnetic field oscillations f = 13.56 MHz , f = 40.68 MHz, f = 433 92 MHz, f = 915 MHz and f = 2400 MHz. The concentration of the drug "Chlorin-e ₆ " in the tumor reaches its maximum value during the first 3-5 hours after its intravenous administration and remains for a day, in other organs and tissues the concentration of the drug sharply decreases over this period of time, which causes maximum damage tumors during HF and microwave electromagnetic hyperthermia during this period of time. After 5 hours, after intravenous administration, Chlorin-e _{6 is} excreted from healthy tissues in comparison with tumor tissues and 2.5 hours after oral administration of succinic acid, it also quickly eliminates from healthy tissues. The result of this is a high fluorescence contrast of the tumor and an increase in its electrophysical properties, i.e. an increase in its conductivity (dielectric loss) relative to surrounding healthy biological tissues, reaching a difference of 3-4 times the magnitude for various tumors. This allows one to specify the boundaries of tumors and the simultaneous electromagnetic hyperthermia of tumor cells with the energy of wave radiation during fluorescence diagnostics, and thus identify and destroy even undetectable tumor formations located in deep layers of a biological object by the energy of wave radiation.

Then the level, after 5 hours, of the sodium salt of Chlorin-e ₆ and the level of succinic acid, gradually decreases in the tumor after 2.5 hours, reaching its initial values 24 hours after the administration of the drug Chlorin- ₆ intravenously (dropwise) with a 30-minute infusion in a semi-darkened room in a single dose of 120 mg / kg of body weight with preliminary dilution 24 hours before the diagnosis and hyperthermia of the tumor tissues of the RF and microwave energy by wave radiation, after which the tumors denature and within 2-4 weeks the decay products of the tumor cells are excreted by the body on its own. Within 5-24 hours after administration of Chlorin-e ₆ and succinic acid, the patient should be isolated from bright sunlight. The patient is allowed to stay in a room with an artificial light source.

Succinic acid (succinates) is a substance obtained during the processing of natural amber. This is a completely safe product with a number of useful qualities. Receive in the form of a white crystalline powder, tasting similar to citric acid.

In the body, succinic acid is active in the form of anions and succinate salts of natural regulators of the body's life. We feel the need for them with increased physical, psycho-emotional, intellectual stress, with various diseases. Succinic acid has a unique effect: it accumulates in those areas that need it, ignoring healthy tissues.

For the treatment of cancer, succinic acid is recommended, which is effectively absorbed by the surfaces of the tumor cells, reduces the hypoxia of the tumor cells and saturates them with oxygen using succinic acid, which can penetrate the tumor cells through cell membranes and can destroy the metachondria of tumor cells and inhibit the growth of tumor tissue by transferring them to oxygen aerobic nutrition, without damage to normal cells. Succinic acid receptors do not have a noticeable toxic or side effect on animal or human cells, and they are selected from the group of these compounds consisting of succinic acid.

A number of researchers claim that succinic acid, which is a prooxidant that neutralizes free radicals, kills cancer cells in the blood and tissues without affecting healthy ones, due to the local oxidative stress-damage process caused by oxidation, cellular DNA and depletion of adenosine triphosphate (ATP) - cell energy source. Succinic acid, among other molecules accompanying it, due to aggressive action, causes a malfunction in the functioning of a particular enzyme responsible for the "nutrition" of cancer cells. UC is a representative of intermediate acids that can accumulate in the cytosol of cells. Tumor cells accumulate, unlike normal ones, a significant amount of succinic acid.

Succinic acid has no side effects and is practically harmless. Its only unpleasant quality is that it can irritate the gastric mucosa, therefore it should not be used on an empty stomach. Succinic acid also has the ability to enhance the therapeutic effect of medicines. Succinic acid acts only where there is a shortage of it, accumulating in damaged places, that is, its effect is targeted, which is practically impossible to say about any drug.

As the studies of the professor of the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences M.N. Kondrashova, the energy capacity of the ATP synthesis process during the oxidation of succinic acid is significantly higher than during the oxidation of any other substrate. That is why many energy-dependent, that is, energy-consuming processes, for example, the accumulation of calcium ions and the provision of hydrogen biosynthesis, even in isolated mitochondria, can occur only during the oxidation of succinic acid. The works of the school M.N. Kondrashova showed that in nature there exist and, if necessary, activate additional pathways for the formation of succinic acid. In particular, such an additional “injection” of succinic acid in a healthy person occurs during intensive work and during the recovery period after exertion, when the demand for rapid reproduction of ATP is especially high.

In hypoxia, the mitochondrial respiratory chain cannot accept hydrogen from any substrate other than succinic acid. After all, it is during its oxidation that hydrogen enters a region of the respiratory chain that is much closer to oxygen. At the same time, even with deep hypoxia, the ability to accept hydrogen is retained. In this case, the oxidation of succinic acid in mitochondria remains one of the few sources of ATP. The additional intake of succinic acid can significantly help the body.

During HF and microwave heating of tumor tissues, the sodium salt of Chlorin-e ₆ is oxidized with succinic acid with the release of hydrogen in an alkaline medium, the reaction is catalyzed by heme of iron, and causes chemiluminescence with the active release of singlet oxygen. If an oxidizing agent - succinic acid with active evolution of hydrogen is added to the alkaline solution of oncell cells, then a glow occurs. In the presence of catalysts, this glow increases and becomes brighter. The role of catalysts for the solution of the sodium salt of the drug Chlorin-e ₆ is carried out by hemin of blood iron and various sodium compounds. These chemical chemiluminescence activators enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. Observed during this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is sodium salts of Chlorin-e ₆ in the presence of oxygen radicals. Under the action of the UC of the oxidizing agent - hydrogen radicals, the formation occurs, which reacts with the superoxide radical forming the internal peroxide (dioxide), which leads to the formation of an excited molecule of the sodium salt of the drug Chlorin- ₆ . The transition of this molecule to the ground initial state is accompanied by emission by a quantum of light. Hydrogen peroxide is the main participant in the formation of free radicals, it is constantly formed in small amounts in the human body, it is a relatively harmless compound, but in the presence of metal ions of variable valence of iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide H ₂ O ₂ a destructive hydroxyl radical is formed JOH, which causes mutations and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl group of the enzymes activates the molecules, under the influence of oxidized succinic acid and sodium salts, Chlorin- ₆ actively enters into a chemical reaction with them, and under the influence of the electromagnetic field of the RF and microwave leads to a bright glow of biological tumor tissues caused by photoelectromagnetic fluorescence.

When placed in an alternating electromagnetic field of high tension and frequency of various biological bodies, they also begin to emit a characteristic radiance of various intensities and colors, from which one can judge the properties of the object under study. The method of “high-frequency photography” (Kirlian effect, kirlianography in honor of the inventor V.K. Kirlian) is now widely known in Russia and abroad as a method of experimental studies of electromagnetic fields and bioenergetic interactions. But the greatest scientific and practical interest is the study of the glow of biological objects in an alternating electromagnetic field of high frequency. explained by the photoelectromagnetic effect of photo-wave radiation and the luminescence of biological objects.

In accordance with modern concepts, aqueous solutions of alkalis and acids in the human body are considered as an associated liquid consisting of separate associated elements - neutral clusters and cluster ions of the general formula (H ₂ O) _n , [(H ₂ O) _n ] ⁺ , [( H ₂ O) _n ] ^- [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ], etc. e, where the number of water molecules bonded into hydrogen bonds can reach n times, according to some authors, hundreds or even thousands of units under the influence of HF and microwave energy. These effects respectively change the electrical conductivity and biophotoluminescence of biological tissues. Changing the position of one structural element (water molecule) under the influence of any external factor or changing the orientation of surrounding neighboring water molecules in the cells provides high sensitivity of the entire water information system to various external influences (electromagnetic, thermal, sound fields, bio-exposure, etc.). In addition, in water clusters, due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, proton (H ⁺ ) migration by the relay mechanism can occur, leading to proton delocalization within the cluster, providing singlet oxygen with a characteristic bright glow, killing cancer cells. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy - information, etc. In addition, aqueous solutions, various chemical elements, is a source of superweak and weak alternating electromagnetic radiation. In this case, the induction of an external electromagnetic field can occur, causing resonance effects of combining (superposition) of external electromagnetic fields with its own fields in biological objects under photo-wave radiation, which can change the structural and informational characteristics of biological objects, 80-90% of which are solutions of water with various chemical impurities and cause their photoluminescence.

Under the influence of a high frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, the molecules N ₂ , H ₂ , O ₂ and CO ₂ are excited, polarized and ionized. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of an object saturated with various chemical solutions, can color the luminous corona in various color schemes. The shape of the glow corona, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body, granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells secrete active forms of singlet oxygen contained in super axide radicals, hydrogen peroxide H ₂ O ₂ , and hydroxyl radical JOH in this case, weak chemiluminescence is observed, which is enhanced repeatedly in the presence of the sodium salt of the drug "Chlorin-e ₆ " and succinic acid. These effects are also amplified many times by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the cell metachondria. This effect of the influence of electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nicolo Tesla, when exposed to pulsed high-frequency energy of vessels with liquids with the ability to emit light and fluorescent lamps that glowed without connecting to electric wires, with bright light in the hands of Nikola Tesla, with whom he also juggled, which caused genuine delight among the audience, while at the same time a phenomenon inexplicable by nature, which only Nicolo Teslo knew.

These factors in biology are called collective stimuli of luminescence that alter the state of blood and tissue phagocytes and their ability to increase the excretion of reactive oxygen species, and accordingly the protective functions of cells.

In cancer cells, aerobic respiration is absent in the mitochondria and is replaced by oxygen-free glycolysis. Sodium salt of Chlorin-e ₆ together with UC, upon entering the cancer cell, inhibits glycolysis, but is not able to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. In order to completely turn off glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or for the sodium salt Chlorin- ₆ and UC to prevail in the substrate over glucose. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In cancer cells, when there is an overabundance of sodium salts of Chlorin-e ₆ and UC, and an additional simultaneous effect on the cells of the RF and microwave electromagnetic hyperthermia, oxidation processes are stimulated, which, when they are overexposed, have a toxic effect on the cancer cells. It can be argued that the effect would be higher if the treatment was based on the sodium salt of Chlorin- ₆ and UC against the background of a complete overlap of the intake of carbohydrates - glucose, as competitors of the sodium salt of Chlorin- ₆ in cancer cells. For this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract turn into glucose, which is essential for the nutrition of cancer cells. With this introduction of cancer cells into artificial glucose fasting, then a person must enter high single doses of 2-3 mg / kg body weight of sodium salt of Chlorin-e ₆ and 2400 mg of UC. The necessary amount of drug administration for HF and microwave treatment of tumors should be taken for their maximum accumulation based on the maximum permissible single megadose of sodium salt of Chlorin- ₆ , not exceeding 3.1 mg and 3000 mg of UC. Under the action of enzymes in the human body, sodium salts of Chlorin-e ₆ and UC accumulate and is determined by the intracellular concentration (level of sensitizer accumulation), its localization in the cell and photochemical activity (quantum yield of generation of singlet oxygen or free radicals), providing a fluorescent tumor contrast and increase its conductivity, relative to surrounding healthy biological tissues. When sodium chloride Chlorin-e ₆ and UC enter the blood vessels of the tumor, which have a large branched network with thin peripheral vessels and a low velocity of blood in them, the blood flow in these vessels of the tumor tissue decreases even more when they are heated, which leads to blood coagulation in the vessels of the tumor tissue, not allowing them to cool, due to the lack of a closed circulatory system. This is a direct cytotoxic effect on tumor cells, disrupting their blood supply, due to damage to the endothelium of the blood vessels of the tumor tissue, due to the hyperthermic effect and cytokine reactions, while macrophages, leukocytes and lymphocytes are activated, leading to tumor necrosis. In the main organs of man, rich in blood vessels closed into the main circulatory system, cooling of healthy border tissues subject to HF and microwave hyperthermia occurs. A “hungry” tumor is maximally saturated with Chlorin- ₆ sodium salt, and UC, as a glycolysis transport, is 3-4 times higher than in normal healthy tissues, and accumulates in a rather large amount on membranes and interstitial fluid.

Sodium salts of Chlorin-e ₆ , and succinic acid are actively imported into the endoplasmic reticules (EPR) (Endoplasmic reticulum, which consists of membranes and sets the direction and active transport of substrates against gradients) of cells using glucose transporters, including succinic acid. It should be noted that the energy processes in cancer cells are transferred from metachondria to the endoplasmic reticulum. It is here that in the EPR both the UC and the sodium salts of Chlorin- ₆ accumulate and the cancer cell environment in this place is significantly different from ordinary cells, they are simply reduced here and the oxidized UC is forced to liberate hydrogen, and together with the sodium salts of Chlorin- ₆ oxidized hymins and transport it to oxygen molecules. From this moment, the destructive effect of UC, Chlorin-e ₆ sodium salt and HF and microwave energy on the cell begins. A “hungry” cancer cell at this time can repeatedly accumulate the sodium salts of hematoporpherin and succinic acid. perceives them on their membrane transporters for glucose. Since there are many times more glucose-consuming receptors in the oncollet than in healthy ones, although the transport systems for delivering glucose, UC, and sodium salt of Chlorin- ₆ to cells are common, this is the Trojan horse for oncocytes. Thus, it is very easy to deceive oncocytes and pump in them the sodium salts of Chlorin- ₆ , and succinic acid to solve the problem of supplying megadoses of the sodium salt of Chlorin- ₆ and UC, followed by processing of RF and microwave energy, then the phenomenon of death of cancer cells will be greatly enhanced .

A “hungry” tumor in the absence of glycolysis is saturated with sodium chloride Chlorin-e ₆ and succinic acid, 3-5 times higher than in normal healthy tissues, stimulates the formation of macrophages and, T-lymphocytes under the action of the enzyme ferrachelase, in a sufficiently large amount on membranes and interstitial fluid. It is this chemical compound that is formed in the process of the interaction of the sodium salt of Chlorin-e ₆ and succinic acid in the internal environment of the body. Under the influence of the oxidizing agent of lipoperikis radicals and the formation of hydrogen, a significantly enhanced temperature effect and the additional action of electromagnetic fields of HF and microwave, active hydrogen radicals are formed, which then reacts with the superoxide radical, forming internal peroxide (dioxide), Н ₂ O ₂ at HF and microwave hyperthermic decomposition, UC of vitamin B ₉ . In this case, there is a multiple amplification in the formation of excited oxygen molecules. The transition of sodium dioxide molecules and succinic acid lipoperoxides from the excited to the ground state is accompanied by the emission of light quanta and a strong blue glow. As a result of these electrochemical reactions associated with a high release of reactive oxygen species and organic free radicals, oncological cells are burned out by the electromagnetic field of the HF and microwave, temperature and electromagnetic photoluminescence.

The method of "selective starvation" of cancer cells superficial and deeply located in the human body, by subsequent administration or administration of various sensitizers, for the selective maximum saturation of tumor cells with highly electroconductive electron-ion solutions of electrophotosensitizers with the maximum separation of electrophysical properties, tumor and healthy tissues, followed by selective exposure to them high-frequency electromagnetic fields in combination with other methods - this is the most relevant scientific and practical direction in the fight against cancer.

A “hungry” tumor is saturated as much as possible, with Chlorin-e ₆ sodium salt and UC stimulates the formation of lipoperoxides with active hydrogen evolution and significantly accelerates this process, under the influence of high-frequency and microwave heating, in a fairly large amount on ricictums and in interstitial fluid. It is these chemical compounds that are formed during the interaction of the sodium salt of Chlorin-e ₆ and UC and the internal environment of the body. A strong oxidizing agent at the allowed frequency of electromagnetic field oscillations f = 13.56 MHz, f = 40.68 MHz, f = 433 92 MHz, f = 915 MHz and f = 2400 MHz together with the sodium salts of Chlorin-e ₆ is a factor or hormone, stimulating the mechanisms of self-destruction and death of cancer cells. The formation of sufficient doses of lipoperoxides with the active release of hydrogen and sodium salts of Chlorin-e ₆ around and inside cancer cells and their apoptosis is possible only with a sufficiently large amount of succinic acid and sodium salts of Chlorin-e ₆ . Under these conditions, the sodium salt of Chlorin-e ₆ and UC can manifest itself as an antioxidant or prooxidant, i.e. an oxidizing agent, including the destructive, rather than creative, property of cancer cells. This is very important in cell energy. Therefore, the sodium salt of Chlorin-e ₆ and UC can be designated as a metabolic switch that accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in the mitochondria and is replaced by glycolysis. UC upon entry into the cancer cell inhibits glycolysis, but is not able to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely turn off glycolysis in tumor cells, glucose access must be completely excluded, or Ul and Chlorin- ₆ sodium salt should prevail over glucose and under the influence of the electromagnetic field of the HF and microwave causing them to heat, with a large release of lipoperoxides and active hydrogen evolution with the formation of the main activators of tumor cell death. In healthy cells, UC and Chlorin-e ₆ sodium salt in small amounts in cytazole show protective antioxidant properties. In cancer cells, when they are overabundant, oxidation processes are stimulated, with the formation of lipoperoxides and active hydrogen and oxygen, which have a stable toxic effect on cancer cells.

The study of biophysical and biochemical mechanisms determines three concepts of the death of cancer cells, one assumes the significance of UC, the other sodium salt of Chlorin-e ₆ and the third RF and microwave hyperthermia. The general combination of these methods will lead to the apparent death of cancer cells.

A number of researchers argue that the minimal molar concentration of succinic acid and sodium salts of Chlorin-e _6, which is a prooxidant (oxidizing compounds that neutralize free radicals), kill cancer cells in the blood and tissues without affecting healthy ones, due to a localized oxidative stress damage process, in the result of oxidation, cellular DNA, and depletion of adenosine triphosphate (ATP), a source of cell energy. Lipoperoxides with the formation of hydrogen, among other molecules accompanying it, aggressive action, causes a malfunction in the functioning of a certain enzyme responsible for the "nutrition" of cancer cells. Sodium salts of Chlorin-e ₆ and UC can accumulate in the cytosol of cells, and with further exposure to HF and microwave energy, they disrupt the endothelium of the blood vessels of tumors and cytokine reactions that stimulate TNF-a, which activate microphages, leukocytes and lymphocytes, and actively damage tumor cells.

Tumor cells, in contrast to normal cells, accumulate a significant amount of theolactone homocysteine (HTL). Prior to insertion of homocysteine into the protein, it becomes a biological defect in the form of (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone practically does not form from it, but conversion to a cancer cell requires significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein synthesizing the cancer cell machine works at full capacity, therefore it is often mistaken. Tue found that reacting with organic acids (Dehydrojuccinic acid), this substance and the chlorin-e ₆ ionic sodium salts, form a highly toxic 3-mercaptopropionic aldehyde (MPA). When succinic acid and Chlorin-e ₆ ionic sodium salts get into a cancer cell saturated with HTL, MPA is formed, which kills cancer cells. By destroying cancer cells, MPA eliminates the source of its formation, therefore, normal cells do not suffer much from them under the action of the sodium salt of Chlorin-e ₆ , succinic acid and HF and microwave energy. In this case, it can be argued that when treating cancer with the sodium salts of Chlorin-e ₆ , UC and HF and microwave energy in the blood, and in various organs of a person, a 100% therapeutic effect will be observed.

Sodium salt Chlorin-e ₆ , and succinic acid in cancer cells is converted, under the influence of temperature, with the formation of the active forms of hydrogen and oxygen and other lipoperoxides. The more sodium salt of Chlorin-e ₆ and UC in the cancer cell, the more lipoperoxides and active forms of hydrogen and oxygen are formed in it, in comparison with healthy cells. Excess lipoperoxides trigger the mechanism of death of cancer cells. The death of cancer cells is initiated by the HF and microwave fields by rapidly heating the cancer cells to 49 ° C, saturated with chlorin- ₆ sodium salt and UC of their rapid oxidative decomposition under the influence of temperature with a large release of lipid peroxides of active forms of hydrogen and oxygen and, which is detrimental to cancer cells.

In healthy cells of the sodium salt of Chlorin- ₆ and UC entering the EPR do not alter the biochemical metabolism in the cells, because pH and redox potential (redox potential) are not suitable for this, and the sodium salts of Chlorin-e ₆ and UC for them will be practically harmless and will be transformed on the glucose conveyor. The environment in the cells is different, the reduced salt and sodium salts of Chlorin-e ₆ and UC, to the maximum, everything is burned and destroyed in the cancer cell during HF and microwave photoelectromagnetic hyperthermia due to lipid peroxidation (POL). In this case, significant damage occurs with the formation of toxic lipid peroxides that damage cell membranes, various organs, mutation of nucleic acids, inoculation of enzymes, destruction of nutrients and cell death. In this case, cell death does not follow the path of apoptosis, but the path of powerful necrosis.

The proposed complex of solutions of the sodium salt of Chlorin-e ₆ and succinic acid on the cell membrane is taken by glucose as cancer cells. The accumulation of sodium salt of Chlorin-e ₆ in the tumor occurs within 3-5 hours after it is administered intravenously, and succinic acid 0.5-2.5 hours after it is taken orally. Then the level of the sodium salt of Chlorin-e ₆ after 72 hours, in the tumor gradually decreases, reaching the initial values 14 days after the administration of the drug Chlorin-e ₆ , and succinic acid 2.5 hours after administration. Chlorin-e ₆ is administered intravenously (drip) with a 30-minute infusion in a semi-darkened room in a single dose of 2-3 mg / kg of body weight with preliminary dilution in 40 ml of sterile physiological solution, the drug is stored in a dark place, 24 hours before the diagnosis and hyperthermia of the tumor tissues of HF and microwave energy by wave radiation, after which the tumors denature and within 2 weeks the decay products of the tumor cells are excreted by the body on its own. Within 3-5 hours after administration of Chlorin-e _{6, the} patient should be isolated from bright sunlight. The patient is allowed to stay in a room with an artificial light source.

With further simultaneous hyperthermia of tumor cells saturated with Chlorin- ₆ sodium salt and succinic acid for 198 sec with wave radiation from the RF and microwave fields, with a heating rate of 0.062 ° C / sec to a final temperature of tumor cells heating of 49 ° C at the allowed vibration frequencies electromagnetic field f = 433 92 MHz, f = 915 MHz or 2450 MHz,

The required amount of the drug, based on the permissible single megadose of sodium salt of Chlorin-e ₆ , before HF and microwave exposure should be 3.2 mg / kg of body weight and ascorbic acid up to 500 mg orally 3 hours before HF and microwave hyperthermia of tumor tissues with wave energy radiation, for 198 sec, with a heating rate of 0.062 ° C / sec to a temperature of 49 ° C, as a result of which the tumors denature and subsequently after 2-4 weeks, the decay products of the tumor cells are excreted by the body independently, naturally, excluding surgical intervention in the human body .

This effect is proposed for the treatment of cancer patients and is explained by the fact that at this time from 3 to 5 hours in normal cells of living biological objects of the sodium salt of Chlorin- ₆ , and succinic acid quickly turns into divalent iron heme and succinic acid lipoperoxides, under the action of the enzyme ferrochelatase, while maintaining a high contrast of the content of the sodium salt of Chlorin-e ₆ and UC in the tumor, which significantly increases its electrical conductivity with a significant change in the dielectric properties of tumors relative to the surrounding healthy biological tissues, reaching this difference many times.

This allows simultaneous fluorescence diagnostics to clarify the boundaries of tumors and simultaneous electromagnetic photodynamic hyperthermia of tumor cells by wave radiation energy with the decomposition of sodium salt of Chlorin- ₆ and UC in lipoperoxide with the release of both active hydrogen and oxygen and hydroxides that can effectively detect and destroy, thus , even undetectable tumor formations located in the deep layers of a biological object.

The main task for researchers is to maximize the effect of the maximum selective absorption of trisulfophthalocyanine hydroxyaluminium by cancer cells with the subsequent subsequent high-frequency irradiation of cancer cells in order to increase the effectiveness of treatment to 100%. It has already been proven that such an effect is possible by the example of disinfection of biological objects from viral, fungal and bacterial infections with HF and microwave energy. Numerous studies conducted by us in the Krasnoyarsk State Agrarian University and VIZR in St. Petersburg have confirmed the 100% effectiveness of disinfecting vegetable seeds and living bioobjects saturated highly conductive electron-ionic solutions of micronutrients of high frequency and microwave energy against viral infections having a similar origin with cancer cells. Partially these works are presented; A.S. No. 563938 of the USSR. A method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bull. Number 25. USSR Certificate No. 950214. The method of presowing treatment of seeds / Tsuglenok N.V. - Registered in the register on 04/14/1982, Bull No. 45. Intensification of thermal processes of seed preparation for sowing with high-frequency and microwave energy: guidelines / N.V. Loam. - M .: Agropromizdat, 1989. Guidelines for the use of HF and microwave energy in the processes of preparing seeds for sowing / N.V. Loam. - M.: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfecting tomato seeds against a viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute for Plant Protection (VIZR). - 1989. -T. 71. - S. 49 - 54. The method of disinfection of egg powder. Patent Number: 1734632. Published: May 23, 1992 Authors: Tsuglenok N.V., Kolmakov Yu.V. IPC: A23v 5/02. Method for preparing medium for diluting sperm of a manufacturer Patent number: 1769422. Published: June 27, 1995. Authors: Tsuglenok, Ostashko, Chess, Silantyev, Kontsedal.

It is proved that oncoviruses under the influence of carcinogens integrate into a healthy cell and dissolve in it over time, turning it into a cancer cell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless; they simply do not exist. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the small penetration depth of the electromagnetic wave of laser emitters does not allow to burn deep malignant tumors.

It is worth noting another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein shell filled with a mixture of nucleic acids and similarly, tumor tissues filled with intercellular fluid solutions determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues .. With increasing temperature with RF and microwave heating in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity and dielectric loss, which further enhances their selective heating and apoptosis of tumor tissues.

A particularly significant factor for the metabolism of cancer cells is the content of glucose or its substitutes, in this case, sodium salts of Chlorin-e ₆ and UC. If the human body has malignant tumors and metastases of stages 3 and 4, which actively and intensively absorb glucose or its substitute - sodium salt of Chlorin-e ₆ and UC, they are converted into ATP in cancer cells much less than in healthy cells, resulting in cancer the cells are very hot and increase the temperature of the human body by 1-2 ° C. This physiological mechanism induces an increase in the temperature of the tumor and surrounding normal tissues. The total temperature increase, at present, is recorded by a microwave radiometer with an accuracy of 0.3 ° C, when determining the temperature, deeply located tumor and healthy tissues.

Most importantly, this method is harmless, does not have special side effects for biological objects.

The biophysical meaning of this method consists in the selective maximum saturation and accumulation of highly electroconducting electron-ion solutions of electrophotosensitizers in tumor cells and in the maximum separation of electrophysical properties, tumor and healthy tissues by decomposition of sodium salt of Chlorin- ₆ and UC and a significant increase in the difference in electrical potentials of tumor and healthy cells in the intercellular medium and on the walls of the reticulum. Reticulum is an electrical circuit where negative charges are evident on one side of the membrane and positive charges on the opposite side, which is why the reticulum is an electric transporter of glucose and other nutrients for cancerous and healthy cells. Therefore, the reticulum is an electric network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of metachondria and energy operator structures on the outer side of the cell - on cilia. Under certain situations in the environment of the cell, these proteins, when discharged, can give an active signal to the reticulum and metachondria. This changes the balance of the existing charges on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are launched. One side of the reticulum membrane is connected to one type of entrance to the metachondria, and the opposite to the exit from it. Thus, a single electric circuit of double active energy management of metachondria is created. The electric field strength on the reticulum keeps under control the work of metachondria. In this case, the metachondria draw in the charges accumulated on one side of the reticulum membrane and remove the opposite charges on the other side of the reticulum membrane. The charges, therefore, do not mix and separate. This is important so that the cells undergo ion exchange. Outwardly, the reticulum is similar to the capacitor plates, the more layers of the plates, the greater its electrical intensity. Between the gaskets is a semiconductor saturated with solutions of the sodium salt of Chlorin-e ₆ and UC. This capacitor, i.e. a powerful dense network of tumor membrane membranes is very clearly visible through a microscope. The number of membranes in tumor cells is much higher than in healthy ones. Accordingly, the density of the tumor tissue and the capacity of the biological electrical capacitor are significantly higher than healthy tissues. When charging such a capacitor on one plate, negatively charged electron particles will collect, and on the other - ions, positively charged particles. Such a charged capacitor can turn into a current source if it is turned off. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which discharges the charge on the metachondria, controlling their activity. Metachondria, in turn, are tuned so that they never allow the charges to reticulum to drop below a critical level. In oncological cells, the charges inside the metachodria sharply decrease and the entire regulatory system is disrupted. This is the main core of the control of the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not basic. As a result of the electrochemical energy of the cell in the reticulum, there is a cycle of substances where the metachondria are the pump. With the lack of this cycle between the reticuli and metachondria due to electroosmosis, there is a suction of substances from outside through the outer membrane and the opening of locks and a sodium pump on it. The medium on the membranes of the reticulum and the alkaline liquid substrate in the tumor cells is restored, due to the excess of minus charges. This determines the chemical equilibrium in pH of conjugated buffer chemical eletroparic substances when the buffer system is discharged or restored. Charges on the plates of the reticulum and metachondria regulate these processes. Chemical processes, in this case, are simply performers, intermediaries. The outer side of the metachondria provides the voltage of the dependent anion channel. This voltage maintenance mechanism, called VDAC, sets the conditions for the reticulum to work. It is here that on the outer side of the membrane is the enzyme Hexokinase II, which ensures the utilization of glucose or its substitutes. Separation, disconnection of the outer mitochondrial membrane (VDAC) and Hexokinase II provides the induction of apoptosis of tumor cells.

Metachondria works by pulling from the reticulum into itself as an electromagnetic pump, the necessary power under high voltage. Without this effect of the highest tension, pulling in nutrients into the cell will not happen. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Oncocytes, unlike normal cells, do not have cilia. This, the most striking, energy level in cancer cells is absent. The only right way in the fight against cancer cells is to find a weak spot in the energy of cancer cells and thereby destroy them. Metachondria determine the degree of charge to ion pumps on the outer membrane of the cell and starter structures that hold charges on the reticulum. These sensory structures can most quickly be damaged and burn out, since metachodria are the most effective electrochemical furnaces. If metachondria is switched off, the voltage gradient of the cell decreases sharply and the processes go on in the cells on a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. A cancer cell does not take quality, since everything is concentrated on a small area of metachondria, but with a larger quantity, much larger than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but at the same time, glucose consumption will be much larger than in healthy tissues.

The membranes - the reticulums and the nuclei of the cell are the same, and the reticulum, as a capacitor, is contoured to the nucleus, with only one of its electron sides and dumps electrons into the nucleus. Thus, the reticulum charge also provides a charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a heavy-duty electrostatic charge inside the nucleus.

The main task for researchers remains to maximize the effect of maximum selective absorption of sodium chloride Chlorin-e ₆ and UC by cancer cells and to increase the effectiveness of treatment by increasing the electrical conductivity of metachondria and reticulums of cancer cells with simultaneous selective RF and microwave cancer hyperthermia cells. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. The electrical conductivity of cancer cells is due to the presence of mobile charged electrons in them on the reticulums and in the nucleus of the cell and ions in the mitochondria of the cell. The value of electrical conductivity depends on the number of electric charges and their mobility. The electrical conductivity of living tissues is determined by the concentration of ions and their mobility, which is different in different tissues, and therefore biological objects have the properties of conductors, semiconductors and dielectrics. In the intercellular fluid, saturated with solutions of the sodium salt of Chlorin-e ₆ and UC, the maximum content of ions is contained and the electrical conductivity of the tumor tissue is high and exceeds 1 S 1m ^-1 . Large protein molecules have a lower electrical conductivity, up to 0.003 S⋅m ^-1 . Intracellular membranes have a conductivity below (1-3⋅10 ^-5 ) cm⋅m ^-1 . The highest conductivity values in the human body are in liquid media (blood, lymph, bile, urine, cerebrospinal fluid and tumor cells (0.6-2.0 S⋅m ^-1 ) and muscle tissue (0.2 S⋅m ^{- 1} ) .Bone, adipose and nerve tissues, in particular coarse-fibrous connective tissues and tooth enamel tissues (10 ^-3 -10 ^-6 S⋅m ^-1 ), have the lowest electrical conductivity. The conductivity of cells and tissues in RF is much more complex and microwave currents.In this case, biological objects have both conductivity and capacitance, which characterize the dielectric constant.The frequency dependence of the electrical parameters and the absorption of electromagnetic energy are determined by the size and shape of the cells, their permeability, the ratio between the volume of cells and intercellular spaces, concentration free ions in cells and the content of free water in them.All of these factors lead to a change in the electrical conductivity of biological objects.

We partially studied this process when exposed to a biological object with tumor tissues of magnetic fields, which were subjected to the daily complex effects of a constant magnetic field with an intensity of 25 μT and an alternating magnetic field with a frequency of 3.1 Hz and an intensity of 5 μT, exposure time of 60 minutes at a time, within 5 days. The proposed method for the impact of constant and variable effects on ion exchange in the mitochondria of cells and on negatively charged electrons on the reticulums and cell nuclei allowed the induction of death of tumor cells using magnetotherapy, which 40%, compared with the control, freed biological objects from tumor cells ( patent No. 2307681, authors: Tsuglenok N.V., Sergeeva E.Yu., Klimatskaya L.G. RU). Therefore, this direction of using magnetic and electromagnetic fields and their impact on the energy of tumor cells deserve special attention, confirmed by researchers from South Korea, who proposed using a powerful magnetic field to destroy tumor cells. In a powerful magnetic field, the tumor begins to kill itself.

A known method of destruction of cancer cells during microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the resonance frequency of the absorption of the tumors is detected. Then they carry out a similar effect on healthy tissues bordering on the tumor and reveal the value of the resonance frequency of absorption of these healthy tissues. Simultaneously with hyperthermia, the values of the resonant frequencies are monitored by the absorption of energy by tumors and healthy tissues, and when the values of the resonant frequencies come closer together, the absorption of energy by tumors and healthy tissues is judged on the effectiveness of the treatment. This method allows to increase the effectiveness of the treatment of tumors by microwave hypothermia when they are heated to 43 ° C.

The main disadvantage of this method is the small difference in the heating of tumor and healthy tissues.

A known method of destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6) the essence of the invention includes the introduction into the tumor localization region of ferromagnetic particles, followed by local induction heating, in the temperature range from 42 ° C to 45 ° C, over time, determined by the type of tumor, its size, localization and type of ferromagnetic particles selected for induction heating, while heating is carried out only at times when the blood supply to the tissue decreases, i.e. at the time of exhalation and diastope of the patient’s heart. The heating range is controlled by a microwave depth thermometer, and heating is carried out automatically, using a computer, in biocontrol mode, according to the algorithms of a mathematical model of fluctuations in the thermal conductivity and heat capacity of the fabric, hysteresis of heating and heat removal.

The main disadvantages of this method is the small localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in various spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method for the destruction of cancer tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, Serdar Deger, Stefan Loening, Wolfgang Lanksch, Roland Felix Journal of Magnetism and Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on the thermolysis of magnetic nanoparticles introduced into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions across cell membranes, as well as induce inducing alternating electric fields that affect the functioning of neural networks in the human body, associated with heating not only magnetic particles, but also all cells, located in the area of introduction of magnetic particles, and a strong spatial heterogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

A known method of short-focus x-ray therapy with a total focal zone of 100-120 Gy and remote gamma-ray therapy for radiation destruction of malignant cells with a total focal zone of 30-40 Gy (see Sh. H. Gantsev. Oncology, M .: Medical Information Agency. 2004, pp. 190-204; Stephen J., Withrow E., MacEwen G. Smal animal clinical oncology - 2001, p. 305-308).

However, this method, despite the prevalence, has the following disadvantages. In the treatment of certain types of malignant neoplasms, for example melanoma, with the help of remote gamma therapy, even in combination with immunotherapy, as experience shows, leads to a 75-90% tumor recurrence, and metastases occur after 2-6 months.

The known method of neuron - capture selective destruction of melanoma (see V.N. Mitin, N.G. Kozlovskaya, AM Arnopolskaya Neuron - capture therapy of oral tumors in dogs. All-Russian Veterinary Journal. 2006. No. 1, pp. 9-10) .

The method involves intravenous administration of L-borphenylalanine, which selectively accumulates in a particular melanoma tumor, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, selective accumulation of L-borphenylalanine in melanoma cells occurs. Upon irradiation of a spatial zone commensurate with a tumor containing L-borphenylalanine by a beam of slow neurons obtained from a neuron guide from a nuclear reactor, melanoma cells are destroyed due to the induced secondary local radiation of boron.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced when using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to serve.

3. Long-term patient exposure for an hour when monitoring the cardiovascular system.

4. The use of general anesthesia.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength that matches the absorption band of the photosensitizer (see Photodynamic therapy / Ed.TJ Dougherty / J. Clin. Laser Med Surg. 1996, Vol. 14, P219-348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of photosensitizer accumulation in tumor cells compared to healthy cells, which is associated with a higher density of blood vessels in the tumor compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, the dye molecules transform into an excited electronic state and, when they collide with oxygen molecules dissolved in biological tissues, transfer it from an unexcited to an excited electronic singlet state with a typical lifetime of several microseconds. During this time, the molecules of singlet oxygen, having passed a characteristic path commensurate with the size of the cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, cell destruction occurs only during exposure to laser radiation in the spatial region of laser beam irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. Used in practice, photosensitizers-phthalacins, porphyrins, chlorins have absorption bands in the ultraviolet or visible spectral range of the photosensitizers, and the lasers used cannot effectively penetrate to a depth of not more than a few millimeters. In addition, the photodynamic method has a low contrast accumulation of photosensitizers in cancer cells.

Closest to the claimed method is the destruction of biological tissue, which consists in introducing ethanol into it using hollow play, characterized in that 95% ethanol is introduced in an amount equal to half the volume of biological tissue to be destroyed, then 5 ml of 20-30% ethanol are introduced, after which is carried out by heating with a high-frequency current with the simultaneous introduction of 20-30% ethanol in an amount equal to the volume of biological tissue to be destroyed. The device contains a high-frequency current generator with two cylindrical electrodes located coaxially relative to each other, internal in the form of a hollow needle through which ethanol is introduced into the tumor (Abstract No. 2006113533 of the patent of the Russian Federation). The disadvantage of this method can be attributed to: the unreasonableness of the selective absorption of ethanol by cancerous and healthy cells, the difficulty of introducing a coaxial electrode into heterogeneous tumors, for organizing uniform heating of tumor tissues that are not equally located from the bare end of the needle.

The objective of the present invention is the local selective destruction of malignant tumors, deeply located in human tissues, previously selectively maximally saturated for 3-5 hours sodium chloride Chlorin-e ₆ and UC for 0.5-2.5 hours.

The proposed method for initiating the death of tumor cells by RF and microwave energy, including pre-saturation of tumor cells with solutions of the sodium salt of Chlorin-e ₆ and succinic acid taken internally by a person, for its maximum accumulation 3-4 times higher than in tumor tissues after 3-5 hours after administration, characterized in that, within 3 days before treatment, the person is transferred to a protein diet to maximize amplification and multiple selective accumulation in the "hungry" tumor tissues of highly conductive electron-ionic solutions of the sodium salt of Chlorin-e ₆ and succinic acid, which increase electrical conductivity and dielectric loss of tumor tissues in comparison with healthy ones, and after 5 hours after taking Chlorin-e ₆ sodium salt and taking succinic acid 2.5 hours before RF and microwave treatment, selective RF and microwave photoelectromagnetic hyperthermia and photoluminescent therapy of tumor tissues with wave energy are performed radiation, with heating rate tumor tissue 0.062 ° C / sec, for 198 sec to a temperature of tumor tissue 49 ° C, when healthy tissue is heated no higher than 40 °

A one-time megadose of 3.1 mg / kg sodium chloride of Chlorin-e ₆ is administered intravenously and a single-dose megadose of 500 mg of succinic acid is taken orally before the HF and microwave treatment of tumor tissues and heating and hyperthermia of tumor tissues saturated with a solution of sodium salt of Chlorin-e ₆ and succinic acid produced by HF and microwave energy of photo-wave radiation, in accordance with the depth of penetration of electromagnetic waves into biological tissues and with the depth of the location of tumor tissues, at the allowed frequencies f = 13.56 MHz - 1100 cm, f = 27 MHz - 545 cm, f = 40 68 MHz - 370 cm, f = 433.92 MHz - 34.5 cm, f = 915 MHz - 16.5 cm and f = 2450 MHz - 6.1 cm, for complete destruction of tumors by photodynamic hyperthermia with minimal destruction of surrounding healthy cells of the biological tissue due to contact heat transfer from the tumor to the boundary layer of healthy tissues, which are heated to a temperature of no higher than 40 ° C, after turning off the RF and microwave energy supply. According to studies on RF and microwave hyperthermia of tumor tissues, at a temperature of 49 ° C, the border between the necrosis zone and healthy tissue is several cells. The destruction zone of tumor tissue includes a small periphery of normal healthy tissues, which excludes the movement of regenerating cells from metastasis by their secondary necrosis during contact heat transfer from heated tumor tissues.

The physical nature of microwave radiation is the physical field of moving electric charges in electric and magnetic fields, which are a single electromagnetic field (EMF), characterized by an oscillation frequency f. The difference is only in the frequency with which electromagnetic oscillations of the corresponding wavelength occur. The biological effect of EMF on a living organism consists in the absorption of energy by biological tissues, characterized by biophysical parameters - dielectric constant and conductivity.

The tissues of the human body, due to the high content of water in them, should be considered as lossy dielectrics. With general body irradiation, EMF energy penetrates to a depth of 0.5 wavelength. The intensity of exposure, exposure and dielectric loss and conductivity characterize the selective absorption of EMF by various tissues at the same EMF radiation density.

where, λ is the wavelength,

c is the propagation velocity of the electromagnetic wave,

f is the frequency of the electromagnetic field. The frequency with which oscillations of the electromagnetic field occur significantly affects the depth of penetration of an electromagnetic wave into a biological object.

The reason is commensurability with various physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, the wavelength of the EMF λ = 33 cm, and at f = 2450 MHz, the wavelength of the EMF λ = 12.2 cm (Table 1).

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with sodium chloride of Chlorin-e ₆ and succinic acid are 3-4 times higher than its content in healthy tissues, respectively, its electrical conductivity is so many times different, i.e. the ability of tumor tissues to conduct an electric current is due to the presence of acid electrolyte in the tumors, free charge carriers - electrically charged particles which, under the influence of an external electric field in the thickness of the tumor, create a conduction current.

Another important parameter of dielectric and semiconductor materials, which are tumors, are dielectric losses. They serve to determine the electric power spent on heating dielectrics and semiconductors in an electromagnetic field. In the reference literature, for the characteristics of the ability of a dielectric to absorb the energy of an alternating electric field, it uses tanδ of the dielectric loss angle and permittivity ε. The physical meaning of tanδ is the presence of dielectric losses leading to a phase shift between the current and voltage, where the angle between them becomes less than 90 ° by an amount, the quantitative wave energy loss is proportional to the dielectric loss ε tanδ.

Losses on electrical conductivity in dielectrics having a low specific volume resistance, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with a changing wave electromagnetic field, converting electrical energy into heat. Tumor tissues are maximally saturated with the sodium salt of Chlorin-e ₆ and UC, in this case they are semiconductors containing several times more charged ions in comparison with surrounding healthy tissues and, accordingly, their heating rate is many times higher than that of surrounding healthy tissues for the same time. In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of the polar water molecules in the direction of the electric field lines. Intramolecular friction occurs, which once again enhances the heating of tumor tissues.

The specific power of the dielectric loss, referred to the unit volume of the dielectric is called the dielectric loss, which can be calculated by the formula:

Ore = E ² f ε * tgδ, * 10 ^-12 W / cm ³

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an electric field. For this, it is necessary to know ε and tanδ of tumor and healthy tissues, and thus very accurately calculate the heating rate to a given temperature of heating of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective absorption of Chlorin-e ₆ sodium salt and UC by the tumor tissues leads to their selective heating of the tumors and electromagnetic photoluminescence to a higher temperature of 50 ° C upon heating, at the same time, of healthy tissues surrounding them to a temperature of 40 ° C, which leads to tumor inactivation tissues and their subsequent destruction, which then, within a few days, are painlessly excreted by the body. The rate of heating by the wave energy of an electromagnetic field depends on the power of dielectric generators and magnetrons.

With an oscillatory power of electromagnetic field generators of 700-850 watts, 200-300 grams of tumor tissue can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors, and their heating temperature is determined by the formula:

where, Co - heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ is the temperature difference of the heating;

t is the heating time, sec.

This formula allows you to select the required total specific power of Oud for RF and microwave heating of tumor tissues of Oud op to a given difference in heating temperatures and the specific power of Oud secreted in healthy tissues determined by the general formula:

P _{beats rev} = P _{beats op} + P _beats

Then the specific power in the irradiation region, taking into account the dielectric properties:

_Of P _ud = (E ² fε tgδ _{op op} + E ² f ε _{zd zd} tgδ) 10 ^-12

Knowing the dielectric properties of the tumor ε _op * tgδ _op and healthy tissues ε _zd * tgδ _zd , it is possible by calculation to determine their heating temperature ΔT to the required set temperatures and determine the heating time t and the total specific power Rud about the irradiated area. (Table 2)

Similarly, knowing the dielectric parameters εtgδ and the specific density of tumor tissues saturated with electrophotosensitizers in biological objects γ g / cm ³ , it is possible to calculate the specific power released in the tumor tissues Rud op and determine the set temperature and calculate the time of their heating by HF and microwave energy, according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells by RF and microwave energy, intended for the complex treatment of cancer patients with tumor tissues with metastases for their hyperthermia in the organs of the human body, characterized in that before treatment the person is transferred to a carbohydrate-free diet for 3 days to create glucose starvation of cancer cells and the maximum saturation of cancer cells with electron-ion solutions, with the subsequent administration to a person of oral sodium chloride "Chlorin- ₆ " in a megadose of 120 mg per person for 3-5 hours and succinic acid orally in a megadose of 2500 mg for 0.5-2 , 5 hours before treatment with a maximum accumulation of drugs in tumor tissues 3-4 times higher than in healthy tissues, selective hyperthermia of tumor tissues with RF energy is carried out in accordance with the depth of their location and the depth of penetration of the electromagnetic wave into the human body = 1100 centimeters, at a permitted frequency f = 13.56 MHz, with a total tumor heating rate at these frequencies 0.062 ° C / sec, for 198 sec until the temperature of tumor tissue heating is 49 ° C, when healthy tissue is heated no higher than 40 ° C.