RU2723883C2 - Method for initiating death of tumour cells with ascorbic acid and hydroside with 3-aminophthalic acid and hf- and microwave energy wave radiation - Google Patents

Abstract

FIELD: medicine.SUBSTANCE: invention refers to medicine and aims at inducing death of tumour cells in living biological objects of ascorbic (AC) and hydrophobic 3-aminophthalic acid (APA) of Galavit preparation and energy of HF- and UHF radiation, known as HF- and UHF-hyperthermia. Described is a method for initiation of tumour cell death, intended for local treatment of oncological patients having tumour tissues with metastases for their hyperthermia in human body organs, characterized by that, that before treatment, person for 3 days is transferred to a non-carbohydrate diet to create glucose starvation and increase electrical conductivity and dielectric losses in "hungry" tumour cells in comparison with healthy, saturated electron-ion solution of hydrazine 3-aminophthalic acid in oral intake in megadose 151 mg for 8 hours and ascorbic acid in megadose of 500 mg for 4 hours for their maximum accumulation in tumour tissues, respectively, in 3–6 times higher than in healthy, selective hyperthermia of tumour tissues is performed by microwave energy in accordance with depth of electromagnetic wave penetration into human body of 6.1 cm on allowed frequencies f = 2450 MHz with a total heating rate of tumour tissues at these frequencies of 0.070 °C/s for 204 s to the temperature of tumour tissues of 51 °C, while heating healthy tissues of not more than 40 °C. This enables fluorescence diagnosis to specify boundaries of tumours and to conduct simultaneous hyperthermia of tumour cells of electromagnetic HF - and microwave energy of photo-wave radiation and detect and destroy thus even undetectable tumour formations located in deep layers of biological object.EFFECT: technical result is thermal death of tumour tissues due to high dielectric contrast of tumour and increasing difference in its conductivity (dielectric loss) relative to surrounding healthy biological tissues, which reaches multiple values for various tumours.1 cl, 2 tbl

Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects of ascorbic acid (AK) and 3-aminophthalic acid hydride (ROS) of the Talavit preparation and the energy of the RF and microwave radiation, known as RF and microwave hyperthermia.

In medicine, hyperthermia is called a significant increase in the human body temperature of more than 40 ° C. Hyperthermia for cancer treatment was used half a century ago. The German doctor von Ardenne opened a "thermal" clinic in a water bath for hopelessly oncologically ill patients, whom he heated to 42 ° C. After this procedure, no more than 17% of people survived, but they were completely cured. The rest were dying, not withstanding such a high temperature. This technology is now used in the United States, where the human body is heated to 42.5 ° C, followed by its return to life. This treatment technology can be effectively used for the selective heating of oncological tissues with RF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

The method for initiating the death of tumor cells by electromagnetic energy of HF and microwave radiation consists in a complex simultaneous exposure to ascorbic and hydroside 3-aminophthalic acids, which include tablets of the preparations “Ascorbic acid” and “Galavit” - Luminol, intended for intravenous administration and oral administration by humans , and wave irradiation with electromagnetic energy of HF and microwave. The drug "Galavit" - Luminol - a crystalline powder of a white-yellow hue. It is very soluble in hydrogen peroxide and other oxidizing agents, and organic liquids. Luminol does not dissolve in water.

After taking a solution of ascorbic acid and 3-aminophthalic acid hydroside and preserving them in a significant amount in the tumor tissues of the human body, it takes time for them to accumulate in the tumor tissues for 2-4 and 6-8 hours. The maximum content of ascorbic acid and hydroside 3-aminophthalic acid in tumor tissues occurs precisely at this time period and it is 3-6 times higher in tumor tissues than in healthy tissues due to selective absorption of acid by tumor tissues.

The famous scientist Launus Pauling conducted scientific data on the positive effect of ascorbic acid (AK) on the slowdown of the cancer process. Some experts explained this by the fact that AK was taken by sick people in large doses in the form of tablets and did not always give a positive effect due to the fact that the concentration of AK in the tumor itself was low. It has been suggested that the tumor suppression mechanism is associated not with AK, but with its oxidized form. This was confirmed by scientific research. In experiments where Dehydro-AK was taken, the results were constantly renewed. These results were published in the 80s and early 90s of the XX century. The most complete description was given in 1991 in the journal of the American Association of Clinical Nutrition. Good results were obtained for leukemia in mice. The skepticism of the scientific world, however, did not allow to pay serious attention to these data. Lonely doctors continued research on the treatment of cancer with vitamin C, without delving into especially the theoretical foundations of this process. A researcher from Canada, John Tewe, tried to uncover the mechanisms of the effect of AK on tumor cells. His final work was published in Cancer letters in 2008. Currently, AK doctors use it as an additional tool that reduces the side effect of chemotherapy.

A known complex for the diagnosis and destruction of tumor tissues by lasers and hydroside of 3-aminophthalic acid converting to aminophthalhydroside. This complex with 3-aminophthalic acid hydroside or its derivative aminophthalic hydroside penetrates into the cell along the pathway with the participation of 3-aminophthalic acid hydroside on the cell membrane, due to the polysaccharides taken by oncocytes as glucose. This complex with 3-aminophthalic acid hydroside or its derivative is used to produce drugs with antitumor activity.

The 3-aminophthalic acid hydroside is oxidized by hydrogen peroxide of the dehydroascorbic acid derivative D-AK in an alkaline medium, the reaction is catalyzed by manganese, and causes chemiluminescence, the strongest that has been observed in the near future. If an oxidizing agent, hydrogen peroxide, is added to an alkaline solution of this hydroside, a bright blue glow flares up. In the presence of catalysts, this glow increases and becomes very bright that it can be used as a light source. The role of catalysts for a solution of 3-aminophthalic acid hydroside is carried out by hemin of blood iron with various compounds of potassium, copper, etc. These chemical chemiluminescence activators enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. Observation with this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is the luminal 3-aminophthalic hydroside in the presence of oxygen radicals. Under the action of an oxidizing agent - a hydroxyl radical, a luminol radical is formed, which reacts with a superoxide radical forming an internal peroxide (dioxide), which lead to the formation of an excited 3-aminophthalate molecule. The transition of this molecule to the ground initial state is accompanied by emission by a quantum of light. Hydrogen peroxide is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, it is a relatively harmless compound, but in the presence of metal ions of variable valence of iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide H ₂ O ₂ a destructive hydroxyl radical is formed JOH, which causes mutations, and inactivation of enzymes and damage to biological membranes of cancer cells. The luminal hydroxyl radical actively enters into a chemical reaction with it, which leads to a bright glow of biological tumor tissues.

Some cells of the body: granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells secrete active forms of oxygen contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and hydroxyl radical JOH in this case, weak chemiluminescence is observed, which increases a thousand times in the presence of luminal - 3-aminophthalic acid hydroside during HF and microwave irradiation of cancer cells. It has been proven that these effects are amplified many times, when exposed to blood vessels and cells, even short-term electrical impulses, causing an increase in the permeability of cell membranes - rituals and stimulation of the release of reactive oxygen species by the cell metachondria, leading to bright glow of tumors.

When placed in an alternating electromagnetic field of high tension and frequency of various biological bodies, they also begin to emit a characteristic radiance of various intensities and colors, from which one can judge the properties of the object under study. The method of “high-frequency photography” (Kirlian effect, kirlianography in honor of the inventor V.K. Kirlian) is now widely known in Russia and abroad as a method of experimental studies of electromagnetic fields and bioenergetic interactions. But the greatest scientific and practical interest is the study of the glow of biological objects in an alternating electromagnetic field of high frequency. explained by the photoelectromagnetic effect of photo-wave radiation and the luminescence of biological objects.

In accordance with modern concepts, aqueous solutions of alkalis and acids in the human body are considered as an associated liquid consisting of separate associated elements - neutral clusters and cluster ions of the general formula (Н ₂ O) _n , [(Н ₂ O) _n ] ⁺ , [( H ₂ O) _n ] ^- , [(NO ₂ ) _n ]. [(H ₂ O ₂ ) _n ], [(KаO ₂ ) _n ] [(СlO ₂ ) _n ], [(СO ₂ ) _n ], etc., where the number of water molecules bound into hydrogen bonds can be up to n times , according to some authors, under the influence of HF and microwave energy, hundreds and even thousands of units. These effects respectively change the electrical conductivity and biophotoluminescence of biological tissues. Changing the position of one structural element (water molecule) under the influence of any external factor or changing the orientation of surrounding neighboring water molecules in the cells provides high sensitivity of the entire water information system to various external influences (electromagnetic, thermal, sound fields, bio-exposure, etc.). In addition, in water clusters due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, the proton (H ⁺ ) can migrate by the relay mechanism, leading to proton delocalization within the cluster, providing singlet oxygen with a characteristic bright glow, killing cancer cells. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy - information, etc. In addition, aqueous solutions, various chemical elements, is a source of superweak and weak alternating electromagnetic radiation. In this case, the induction of an external electromagnetic field can occur, causing resonance effects of combining (superposition) of external electromagnetic fields with its own fields in biological objects under photo-wave radiation, which can change the structural-informational characteristics of biological objects, 80-90% of which are solutions of water with various chemical impurities and cause their photoluminescence.

Under the influence of a high frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, the molecules N ₂ , H ₂ , O ₂ and CO ₂ are excited, polarized and ionized. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of an object saturated with various chemical solutions, can color the luminous corona in various color schemes. The shape of the glow corona, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells secrete active forms of singlet oxygen contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and hydroxyl radical JOH in this case, weak chemiluminescence is observed, which is amplified many times . When placed in an alternating electromagnetic field of high tension and frequency of various biological bodies, they begin to emit a characteristic radiance of various intensities and colors, which can be used to judge the properties of the object under study. The method of "high-frequency photography" (the Kirlian effect, kirlianography in honor of the inventor V.K. Kirlian) is now widely known in Russia and abroad as a method for experimental studies of electromagnetic fields and bioenergetic interactions [1]. But the greatest scientific and practical interest is the study of the glow of biological objects in an alternating electromagnetic field of high frequency.

In accordance with modern concepts, aqueous solutions of alkalis and acids in biological objects are considered as an associated liquid [7], consisting of separate associated elements — neutral clusters and cluster ions of the general formula (Н ₂ O) _n , [(Н ₂ O) _n ] ⁺ , [(H ₂ O) _n ] ^- , [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ], etc., where the number of water molecules bound into hydrogen bonds can reach n times, according to some authors, hundreds or even thousands of units [8]. Changing the position of one structural element (water molecule) under the influence of any external factor or changing the orientation of surrounding neighboring water molecules provides high sensitivity of the entire water information system to various external influences (electromagnetic, thermal, sound fields, bio-exposure, etc.).

In addition, in water clusters due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, proton (H ⁺ ) migration can occur via the relay mechanism, leading to proton delocalization within the cluster. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous chemical solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-information, etc. [10]. In addition, aqueous solutions of various chemical elements is a source of superweak and weak alternating electromagnetic radiation. In this case, the induction of the corresponding electromagnetic field and resonance effects of combining (superposition) of electromagnetic fields that can change the structural and informational characteristics of biological objects, 80-90% consisting of water with various chemical impurities, can occur.

Under the influence of a high frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, the molecules N ₂ , H ₂ , O ₂ and CO ₂ are excited, polarized and ionized. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of the object, can color the luminous corona in various colors. The shape of the glow corona, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object with the release of active oxygen forms in it.

Some cells of the body granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells secrete active forms of oxygen contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and hydroxyl radical JOH in this case, weak chemiluminescence is observed, which increases many times the presence of RF and microwave radiation. These effects are also amplified many times by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes — riticulomas and stimulation of the release of reactive oxygen species by the cell metachondria.

This effect of the influence of electric pulses at the beginning of the 19th century was successfully demonstrated to the public by Nicolo Tesla, by irradiating high-frequency pulsed energy from an open antenna of an RF generator located in a room of irradiated vessels with liquids that can emit light and fluorescent lamps that glowed with bright light without connecting to electric wires in the hands of Nikola Tesla, with whom he also juggled. This caused genuine enthusiasm among the audience, while this phenomenon inexplicable in nature. These factors in biology are called collective stimuli in the presence of luminol, which changes the state of blood and tissue phagocytes and their ability to increase the release of reactive oxygen species, and, accordingly, the protective functions of cells.

The main task for researchers remains to maximize the effect of selective absorption of D-AK, AK and ROS by cancer cells and increase the effectiveness of treatment. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects.

If treatment with powerful doses of AK and ROS were taken as the basis for a complete overlap of the intake of carbohydrates - glucose and further irradiation of HF and microwave energy of cancer cells, the effect of electromagnetic photoluminescence and their damage would be even higher. For this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract turn into glucose, which is essential for the nutrition of cancer cells. With this introduction of cancer cells into artificial glucose "starvation", then a person needs to take high doses of AK and ROS Galavit, better than its oxidized form of AK - Dehydroascorbic acid (D-AK) and 3-aminophthalic acid hydrazide. When they enter the blood vessels of the tumor, the blood thickens, the tumor, which has a large branched network with thin peripheral vessels and a low speed of blood movement in them, decreases even more when heated, which leads to blood coagulation in the vessels of the tumor tissues, not allowing them to cool, due to the absence of closed circulatory system. In the main organs of man, rich in venous vessels, closed into the main circulatory system, cooling of healthy border tissues subject to HF and microwave hyperthermia occurs. A “hungry” tumor is maximally saturated with Dehydroascorbic and hydrazide 3-aminophthalic acids, several times higher than in normal healthy tissues, it stimulates the formation of hydrogen peroxide, in a large enough amount in interstitial and intercellular fluid. It is this chemical compound that is formed during the interaction of the drug AK and Galavit in the internal organs of the body. Hydrogen peroxide and lipoperoxide are factors that stimulate self-destruction mechanisms and the death of cancer cells due to the large release of active oxygen. The formation of sufficient doses of hydrogen peroxide and other lipoperoxides around and inside the cancer cells and their apoptosis is possible only with a sufficiently large amount of ascorbic acid and hydrazide 3-aminophthalic acid. Under these conditions, D-AK and ROS can manifest themselves as an antioxidant or prooxidant, i.e. an oxidizing agent, including the destructive, rather than creative, property of cancer cells. This is very important in cell energy. Therefore, AK and ROS can be described as a metabolic switch that accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in the mitochondria and is replaced by glycolysis. AK and ROS, upon entering the cell, inhibits glycolysis, but is not able to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. In order to completely turn off glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or for AK and ROS to prevail over glucose in the substrate. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In cancer cells, when there is an overabundance of it, it stimulates the processes of oxidation, with the formation of hydrogen peroxide and lipoperoxides, which, when they are abundant, have a toxic effect on cancer cells.

Dehydroascorbic acid and hydrazide 3-aminophthalic acid are actively imported into endoplasmic reticules (EPR) (endoplasmic reticulum consisting of membranes and setting the direction and active transport of substrates against gradients) of cells using glucose transporters. It should be noted that the energy processes in cancer cells are transferred from metachondria to the endoplasmic reticulum. It is here that D-AA and ROS accumulate in the EPR and the onccell medium in this place differs significantly from ordinary cells. 3-aminophthalic acid hydrazide is simply re-reduced here and here D-AK is obviously forced to recover to AK. From this moment, the destructive effect of AK and ROS on the cell begins. A “hungry” cancer cell at this time can repeatedly accumulate D-AK and ROS, perceives them on their membrane transporters for glucose. Since there are many times more glucose-consuming receptors in the oncollet than in healthy ones, although the transport systems for the delivery of glucose and D-AK and ROS to the cells are common, this is the Trojan horse for oncocytes. Thus, it is very easy to deceive oncocytes and pump D-AK and ROS into them, with the solution of the problem of supplying megadoses of D-AK and ROS, and then the phenomenon of death of oncocytes will be strengthened many times.

A hungry "tumor is maximally saturated with 3-aminophthalic and ascorbic acid hydrazide, 3-6 times higher than in normal healthy tissues, it stimulates the formation of macrophages and T-lymphocytes under the action of the ferrachelase enzyme, in a sufficiently large amount on membranes and interstitial fluid. a chemical compound is formed during the interaction of 3-aminophthalic acid hydrazide, ascorbic acid and the body’s internal environment Under the influence of the oxidizing agent of the hydroxyl radical and hydrogen peroxide, a significantly enhanced temperature effect and additional action of the electromagnetic fields of the RF and microwave, hydrogen and oxygen radicals are formed - luminal, which then reacts with superoxide radicals that accelerate the formation of internal peroxide (dioxide) and hydrogen peroxide H ₂ O ₂ when they are hyperthermally decomposed by RF and microwave fields. In the presence of AA vitamin C, there is a multiple increase in the formation of excited 3-aminophthalate molecules. The transition of this molecule to the ground state is accompanied by the emission of light quanta, and a strong blue glow. As a result of these chemical reactions associated with a high release of active forms of hydrogen and oxygen and organic free radicals, cancer cells are burned.

The method of "selective starvation" of cancer cells superficial and deeply located in the human body, by subsequent administration or administration of various sensitizers, to selectively saturate tumor cells with highly electroconductive electron-ion solutions of electrophotosensitizers with the maximum separation of electrophysical properties, tumor and healthy tissues, followed by selective exposure to them high-frequency electromagnetic fields in combination with other methods - this is the most relevant scientific and practical direction in the fight against cancer.

The study of biophysical and biochemical mechanisms determines three concepts of cancer cell death, one assumes the importance of D-AK, AK and the other ROS, and the third electromagnetic photodynamic effect of HF and microwave energy on tumor tissues saturated with D-AK and AK, which in three cases leads to apparent death cancer cells.

Tumor cells accumulate, in contrast to normal cells, a significant amount of theolactone homocysteine (HTL). Prior to insertion of homocysteine into the protein, it becomes a biological defect in the form of (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone practically does not form from it, but conversion to a cancer cell requires significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein synthesizing the cancer cell machine works at full capacity, therefore it is often mistaken. Tue found that reacting with dehydroascorbic acid, this substance forms a highly toxic 3-mercaptopropionic aldehyde (MPA). When dehydroascorbic acid enters the HTL-saturated cancer cell and 3-aminophthalic acid hydrazide forms MPA, which kills cancer cells. By destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer much from it under the action of 3-aminophthalic acid hydrazide. In this case, it can be argued that in the treatment of cancer of Dehydroascorbic acid and hydrazide with 3-aminophthalic acids obtained by the oxidation of AA and ROS, both in the blood and in various organs of a person, a therapeutic effect is observed.

Tumor cells accumulate, in contrast to normal cells, a significant amount of theolactone homocysteine (HTL). Before inserting homocysteine into the protein, it becomes a biological defect in the form of (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone practically does not form from it, but conversion to a cancer cell requires significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein synthesizing the cancer cell machine works at full capacity, therefore it is often mistaken. Tue found that reacting with dehydroascorbic acid, this substance forms a highly toxic 3-mercaptopropionic aldehyde (MPA). When dehydroascorbic acid enters an NTB-saturated cancer cell, MPA is formed, which kills cancer cells. By destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer much from it.

In this case, it can be argued that in the treatment of cancer with Dehydroascorbic acid and 3-aminophthalic acid hydrazide obtained as a result of their oxidation, and the formation of hydrogen peroxide in tumor cells, under the influence of electromagnetic energy of HF and microwave electromagnetic hyperthermia and photoluminescence, 100% resistant therapeutic Effect. It has already been proven that such an effect is possible by the example of disinfection of biological objects from viral, fungal and bacterial infections with HF and microwave energy. Numerous studies conducted by us in the Krasnoyarsk State Agrarian University and VIZR in St. Petersburg have confirmed the 100% effectiveness of disinfecting vegetable seeds and living bioobjects saturated highly conductive electron-ionic solutions of micronutrients of high frequency and microwave energy against viral infections having a similar origin with cancer cells.

A.S. No. 563938 of the USSR. A method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bull. No. 25. USSR Certificate No. 950214. The method of presowing treatment of seeds / Tsuglenok N.V. - Registered in the register 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with energy of high-frequency and microwave: methodical recommendations / N.V. Loam. - M .: Agropromizdat, 1989. Guidelines for the use of HF and microwave energy in the processes of preparing seeds for sowing / N.V. Loam. - M.: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfecting tomato seeds against a viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute for Plant Protection (VIZR). - 1989. -T. 71. - S. 49-54. The method of disinfection of egg powder. Patent Number: 1734632. Published: May 23, 1992 Authors: Tsuglenok N.V. Kolmakov Yu.V. IPC: А23в 5/02. Method for preparing a medium for diluting sperm of a manufacturer Patent number: 1769422. Published: June 27, 1995. Authors: Tsuglenok, Ostashko, Chess, Silantyev, Kontsedal.

Most importantly, this method is harmless, does not have special side effects for biological objects.

It is proved that oncoviruses under the influence of carcinogens integrate into a healthy cell and dissolve in it over time, turning it into a cancer cell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless; they simply do not exist. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the small penetration depth of the electromagnetic wave of laser emitters does not allow to burn deep malignant tumors.

It is worth noting another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein shell filled with a mixture of nucleic acids and similarly tumor cells filled with intercellular fluid solutions determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. HF and microwave heating in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity and dielectric loss, which further enhances their selective heating and optoptosis of tumor tissues.

This healing effect is due to the fact that at this time from 6 to 8 hours in normal cells of living biological objects, ascorbic acid and 3-aminophthalic acid hydrazide quickly turn into dehydroascorbic acid and aminophthalhydrazide and ferrous heme, under the action of the ferrochelatase enzyme, while maintaining high contrast the maximum content of dehydroascorbic acid and 3-aminophthalic acid hydrazide in tumors is 3-6 times higher than in healthy tumors and their further conversion to ascorbic and aminophthalhydroside in tumors, which significantly increases their electrical conductivity with a significant increase in the dielectric properties of tumors relative to surrounding healthy biological tissues reaching this difference several times.

With further electromagnetic RF and microwave hyperthermia and photoluminescence of tumor cells saturated with ascorbic acid and 3-aminophthalic acid hydrazide for 204 seconds, the wave radiation of the RF and microwave fields, with a permitted frequency of electromagnetic field oscillations f = 433 92 MHz, f = 915 MHz or 2450 MHz, with a heating rate of 0.07 ° C / s to a final temperature of heating of tumor cells of 51 ° C, they are destroyed.

This proposed technology allows simultaneous fluorescence diagnostics to clarify the boundaries of tumors and simultaneous hyperthermia of tumor cells by the energy of wave radiation with the decomposition of AA and ROS into hydrogen peroxide and hydroxides, which can effectively detect and destroy even undetectable tumor formations located in deep layers of biological object.

The biophysical meaning of this method consists in the selective maximum saturation and accumulation of highly electroconducting electron-ion solutions of electrophotosensitizers in tumor cells and in the maximum separation of the electrophysical properties of tumor and healthy tissues of saturated ascorbic and 3-aminophthalic acid hydrazide, and a significant increase in the difference in the electrical potentials of tumor cells and healthy cells in the intercellular medium and on the walls of the reticulum. Reticulum is an electrical circuit where negative charges are evident on one side of the membrane and positive on the opposite side, so the reticulum is an electric transporter of glucose and other nutrients for cancerous and healthy cells. Therefore, the reticulum is an electric network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of metachondria and energy operator structures on the outer side of the cell - on cilia. Under certain situations in the environment of the cell, these proteins, when discharged, can give an active signal to the reticulum and metachondria. This changes the balance of the existing charges on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are launched. One side of the reticulum membrane is connected to one type of entrance to the metachondria, and the opposite to the exit from it. Thus, a single electric circuit of double active energy management of metachondria is created. The electric field strength on the reticulum keeps under control the work of metachondria. In this case, the metachondria pull in the charges accumulated on one side of the reticulum membrane and remove the opposite charges on the other side of the reticulum membrane. The charges are thus not mixed and divided. This is important so that the cells undergo ion exchange. Outwardly, the reticulum is similar to the capacitor plates, the more layers of the plates, the greater its electrical intensity. Between the gaskets is a semiconductor saturated with Dehydroascorbic, ascorbic acids and 3-aminophthalic acid hydrazide. This capacitor, i.e. a powerful dense network of tumor membrane membranes is very clearly visible through a microscope. The number of membranes in tumor cells is much higher than in healthy ones. Accordingly, the density of the tumor tissue and the capacity of the biological electrical capacitor are significantly higher than healthy tissues. When charging such a capacitor on one plate, negatively charged electron particles will collect, and on the other - ions, positively charged particles. Such a charged capacitor can turn into a current source if it is turned off. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which discharges the charge on the metachondria, controlling their activity. Metachondria, in turn, are configured so that they never allow the charges to reticulum to drop below a critical level. In oncological cells, the charges inside the metachodria sharply decrease and the entire regulatory system is disrupted. This is the main core of the control of all electrochemical energy of the cell. Therefore, chemical processes are always secondary and not basic. As a result of the electrochemical energy of the cell in the reticulum, there is a cycle of substances where the metachondria are the pump. With the lack of this cycle between the reticuli and metachondria due to electroosmosis, there is a suction of substances from outside through the outer membrane and the opening of locks and a sodium pump on it. The medium on the membranes of the reticulum and the alkaline liquid substrate in the tumor cells is restored, due to the excess of minus charges. This determines the chemical equilibrium in pH of conjugated buffer electro-chemical substances when the buffer system is discharged or restored. Charges on the plates of the reticulum and metachondria regulate these processes. Chemical processes, in this case, are simply performers, intermediaries. The outer side of the metachondria provides the voltage of the dependent anion channel. This voltage maintenance mechanism is called VDAC, which sets the conditions for the reticulum to function. It is here that on the outer side of the membrane is the enzyme Hexokinase I, which ensures the utilization of glucose. The maximum separation, separation of the outer mitochondrial membrane (VDAC) and Hexokinase II tumor and healthy cells provides the induction of apoptosis of tumor cells.

Metachondria works by pulling from the reticulum into itself as an electromagnetic pump, the necessary power under high voltage. Without this effect of the highest tension, pulling in nutrients into the cell will not happen. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Oncocytes, unlike normal cells, do not have cilia. This, the most striking, energy level in cancer cells is absent. The only right way is to maximize the separation of properties, oncological and healthy tissues and finding a weak spot in the energy of cancer cells and thereby destroy them. Metachondria set the degree of charge to ion pumps on the outer membrane of the cell and starter structures that hold charges on the reticulum. These sensory structures can most quickly be damaged and burn out, since metachodria are the most effective electrochemical furnaces. If metachondria is switched off, the voltage gradient of the cell decreases sharply and the processes go on in the cells on a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. A cancer cell does not take quality, since everything is concentrated on a small area of metachondria, but in a larger quantity, much larger than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but at the same time, glucose consumption will be much larger than in healthy tissues.

The membranes of the reticulum and the nucleus of the cell are the same; moreover, the reticulum, as a capacitor, is contoured to the nucleus by only one of its electron sides and dumps electrons into the nucleus. Thus, the reticulum charge also provides a charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a heavy-duty electrostatic charge inside the nucleus.

In healthy cells, D-AK and ROS, entering the EPR will not be restored to AK and ROS, because pH and redox potential (redox potential) are not suitable for this, and D-AK and ROS for them will be practically harmless and transform on the glucose conveyor. In cancer cells, the environment is different, converted to ascorbic acid and 3-aminophthalic acid hydrazide, which try to burn and destroy everything in the cancer cell to the maximum due to lipid peroxidation (POL). In this case, significant damage occurs with the formation of toxic lipid peroxides that damage cell membranes, various organs, mutation of nucleic acids, inoculation of enzymes, destruction of nutrients and cell death. In this case, cell death does not follow the path of apoptosis, but overt necrosis.

During high-frequency and microwave heating, ascorbic acid and 3-aminophthalic acid hydrazide in cancer cells are converted to dehydroascorbic acid and aminophthalhydroside under the influence of temperature, with the formation of hydrogen peroxide and other lipoperoxides. The more dehydroascorbic acid and aminophthalhydroside in the cancer cell, the more lipoperoxides and hydrogen peroxide are formed in it, compared to healthy cells. An excess of hydrogen peroxide and lipoperoxide triggers the electromagnetic photoluminescent mechanism of cancer cell death. The death of cancer cells is initiated by the rf and microwave field by rapid heating and electromagnetic photoluminescence of cancer cells by heating them to 51 ° C, saturated D-AK, AK and ROS, their rapid oxidative decomposition under the influence of temperature with a large release of hydrogen peroxide and lipoperoxides causing photoluminescence with large the release of active oxygen, which is detrimental to cancer cells.

The most complete accumulation of ascorbic and 3-aminophthalic acid hydrazide in the tumor occurs within 6-8 hours after ingestion. Then the level of ascorbic and 3-aminophthalic acid hydrazide in the tumor gradually decreases, reaching the initial values 24 hours after taking the drug. Galavit preparations are taken orally at the rate of a megadose of 150 mg 8 hours before the treatment of HF and microwave energy and ascorbic acid with a megadose of 500 mg orally 3 hours before the HF and microwave hyperthermia of tumor tissues with wave radiation energy, after which, with a heating rate of 0, 07 ° C / sec to a temperature of 51 ° C, the tumors denature and subsequently the decay products of the tumor cells are excreted within 2-4 weeks by the body independently, naturally, excluding surgery in the human body.

A number of researchers argue that the minimum molar concentration of AA and ROS, which are prooxidants (oxidative compounds that neutralize free radicals), kill cancer cells in the blood and tissues without affecting healthy cells due to the local oxidative stress-damage process resulting from oxidation of cellular DNA and depletion of adenosine triphosphate (ATP), a cell energy source. Hydrogen peroxide and other lipoperoxides, among other molecules accompanying it, aggressive action, causes a malfunction in the functioning of a certain enzyme responsible for the "nutrition" of cancer cells under the action of photoelectromagnetic RF and microwave energy. AK and ROS are representatives of intermediate acids that can accumulate in the cytosol of tumor cells and kill them under the influence of RF and microwave energy.

The main task for researchers remains to maximize the effect of selective maximum absorption of ascorbic acid and 3-aminophthalic acid hydrazide by cancer cells and increase the effectiveness of treatment by increasing the electrical conductivity of metachondria and reticulums of cancer cells. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. The electrical conductivity of cancer cells is due to the presence of mobile charged electrons in them on the reticulums and in the nucleus of the cell and ions in the mitochondria of the cell. The value of electrical conductivity depends on the number of electric charges and their mobility. The electrical conductivity of living tissues is determined by the concentration of ions and their mobility, which is different in different tissues, and therefore biological objects have the properties of conductors, semiconductors and dielectrics. In the intercellular fluid, contains the maximum content of ions and the electrical conductivity of the tumor tissue is high and is more than 1 S 1m-1. Large protein molecules have a lower electrical conductivity, up to 0.003 S⋅m-1. Intracellular membranes have a conductivity lower than (1-3 10 ^-5 ) cm⋅m-1. The highest conductivity values in the human body are in liquid media (blood, lymph, bile, urine, cerebrospinal fluid and tumor tissue 0.6–2.0 cm⋅m-1) and muscle tissue and (0.2 cm⋅m- 1). Bone, adipose, and nerve tissues have the lowest electrical conductivity, especially coarse-fiber connective tissue and tooth enamel tissue (10 ^-3 -10 ^-6 S cm-1).

It is worth noting another very important biophysical process - an increase in the electrical conductivity of tumor tissues saturated with AA and 3-aminophthalic acid due to a significant increase in the concentration of ions and electrons and their mobility, which increase the electrical conductivity with increasing temperature in the tumor tissues under the influence of RF and microwave energy. With increasing temperature in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity.

A much more complex nature is the electrical conductivity of tumor cells with saturated ascorbic acid and hydrazide 3-aminophthalic acid and tissues at high and microwave currents. In this case, biological objects have both conductivity and capacity, which characterizes the dielectric constant. The frequency dependence of electrical parameters and the absorption of electromagnetic field energy are determined by the size and shape of the cells, their permeability, the ratio between the volume of cells and intercellular spaces, the concentration of free ions in the cells and the content of free water in them. All these factors lead to a change in the electrical conductivity of biological objects. A particularly significant factor for the metabolism of cancer cells is the content of glucose or its substitutes, in this case ascorbic acid and 3-aminophthalic acid hydrazide. If the human body has malignant tumors and metastases of stages 3 and 4, which actively and intensively absorb glucose or its substitute, ascorbic acid and 3-aminophthalic acid hydrazide, they are converted to ATP in cancer cells much less than in healthy cells, as a result of which, cancer cells are very hot and increase the human body temperature by 1-2 ° C. This physiological mechanism induces an increase in the temperature of the tumor and surrounding normal tissues. The total temperature rise in the human body is currently recorded by a microwave radiometer, which allows controlling the temperature of various organs deep in the human body with an accuracy of 0.3 ° C.

This process was partially studied by us when exposed to a biological object with tumor tissues, which were subjected to complex daily exposure to a constant magnetic field with an intensity of 25 μT and an alternating magnetic field with a frequency of 3.1 Hz and an intensity of 5 μT, exposure time of 60 minutes per day at a time 5 days. The proposed method for the impact of constant and variable effects on ion exchange in the mitochondria of cells and on negatively charged electrons on the reticulums and cell nuclei allowed the induction of death of tumor cells using magnetotherapy, which 40%, compared with the control, freed biological objects from tumor cells ( Patent No. 2307681, authors: Tsuglenok N.V., Sergeeva E.Yu., Klimatskaya L.G. K.Ts). Therefore, this direction of using magnetic and electromagnetic fields and their impact on the energy of tumor cells deserve special attention, confirmed by researchers from South Korea, who proposed using a powerful magnetic field to destroy tumor cells. In a powerful magnetic field, the tumor begins to kill itself.

A known method of destruction of cancer cells during microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the resonance frequency of the absorption of the tumors is detected. Then they carry out a similar effect on healthy tissues bordering on the tumor and reveal the value of the resonance frequency of absorption of these healthy tissues. Simultaneously with hyperthermia, the values of the resonant frequencies are monitored by the absorption of energy by tumors and healthy tissues, and when the values of the resonant frequencies come closer together, the absorption of energy by tumors and healthy tissues is judged on the effectiveness of the treatment. This method allows to increase the effectiveness of the treatment of tumors by microwave hypothermia when they are heated to 43 ° C.

The main disadvantage of this method is the small difference in the heating of tumor and healthy tissues.

A known method of destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6) the essence of the invention includes the introduction of ferromagnetic particles into the region of tumor localization, followed by local induction heating, in the temperature range from 42 ° C to 45 ° C, over time, determined by the type of tumor, its size, localization and type of ferromagnetic particles selected for induction heating, while heating is carried out only at times when the blood supply to the tissue decreases, i.e. at the time of exhalation and diastope of the patient’s heart. The heating range is controlled by a microwave depth thermometer, and heating is carried out automatically, using a computer, in biocontrol mode, according to the algorithms of a mathematical model of fluctuations in the thermal conductivity and heat capacity of the fabric, hysteresis of heating and heat removal.

The main disadvantages of this method is the small localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in various spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, Serdar Deger, Stefan Loening, Wolfgang Lanksch, Roland Felix Journal of Magnetism and Magnetic Materials 225 (2001) 118-126.

The destruction of cancer cells is based on the thermolysis of magnetic nanoparticles introduced into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions across cell membranes, as well as induce inducing alternating electric fields that affect the functioning of neural networks in the human body, associated with heating not only magnetic particles, but also all cells, located in the area of introduction of magnetic particles, and a strong spatial heterogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

There is a method of close-focus x-ray therapy with a total focal zone of 100-120 Gy and remote gamma therapy for radiation destruction of malignant cells with a total focal zone of 30-40 Gy (see Sh. H. Gantsev. Oncology, M .: Medical News Agency. 2004, pp. 190-204; Stephen J., Withrow E., MacEwen G. Smal animal clinical oncology - 2001, p. 305-308).

However, this method, despite the prevalence, has the following disadvantages. In the treatment of certain types of malignant neoplasms, for example melanoma, with the help of remote gamma therapy, even in combination with immunotherapy, as experience shows, leads to a 75-90% tumor recurrence, and after 2-6 months metastases occur.

The known method of neuron - capture selective destruction of melanoma (see V.N. Mitin, N.G. Kozlovskaya, A.M. Arnopolskaya Neuron - capture therapy of oral tumors in dogs. All-Russian Veterinary Journal. 2006. No. 1, p. 9 -ten).

The method involves intravenous administration of L-borphenylalanine, which selectively accumulates in a particular melanoma tumor, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, selective accumulation of L-borphenylalanine in melanoma cells occurs. Upon irradiation of a spatial zone commensurate with a tumor containing L-borphenylalanine by a beam of slow neurons obtained from a neuron guide from a nuclear reactor, melanoma cells are destroyed due to the induced secondary local radiation of boron.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced when using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to serve.

3. Long-term patient exposure for an hour when monitoring the cardiovascular system.

4. The use of general anesthesia.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength that matches the absorption band of the photosensitizer (see Photodynamic therapy / Ed. T. J. Dougherty / J. Clin. Laser Med Surg. 1996, Vol. Surg. 14, P 219-348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of photosensitizer accumulation in tumor cells compared to healthy cells, which is associated with a higher density of blood vessels in the tumor compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, the dye molecules transform into an excited electronic state and, when they collide with oxygen molecules dissolved in biological tissues, transfer it from an unexcited to an excited electronic singlet state with a typical lifetime of several microseconds. During this time, the molecules of singlet oxygen, having passed a characteristic path commensurate with the size of the cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, cell destruction occurs only during exposure to laser radiation in the spatial region of laser beam irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. Used in practice, photosensitizers-phthalacins, porphyrins, chlorins have absorption bands in the ultraviolet or visible spectral range of the photosensitizers, and the lasers used cannot effectively penetrate to a depth of not more than a few millimeters. In addition, the photodynamic method has a low contrast accumulation of photosensitizers in cancer cells.

Closest to the claimed method is the destruction of biological tissue, which consists in introducing ethanol into it using hollow play, characterized in that 95% ethanol is introduced in an amount equal to half the volume of biological tissue to be destroyed, then 5 ml of 20-30% ethanol are introduced, after which is carried out by heating with a high-frequency current with the simultaneous introduction of 20-30% ethanol in an amount equal to the volume of biological tissue to be destroyed. The device contains a high-frequency current generator with two cylindrical electrodes located coaxially relative to each other, internal in the form of a hollow needle through which ethanol is introduced into the tumor (Abstract No. 2006113533 of the patent of the Russian Federation). The disadvantage of this method can be attributed to: the unreasonableness of the selective absorption of ethanol by cancerous and healthy cells, the difficulty of introducing a coaxial electrode into heterogeneous tumors, for organizing uniform heating of tumor tissues that are not equally located from the bare end of the needle.

The objective of the present invention is the local selective destruction of malignant tumors deep in human tissues, previously selectively saturated with ascorbic acid for 2-4 hours and 3-aminophthalic acid hydrazide for 6-8 hours, while after 8 hours of administration and subsequent administration of AK 4 hours before the selective HF and microwave heating of the tumor tissue to a temperature of 51 ° C with a heating rate of 0.7 ° C / s for a heating time of 204 seconds.

A method for initiating the death of tumor cells by RF and microwave energy, including a one-time human intake of solutions of ascorbic acid 400 mg and 3-aminophthalic acid hydrazide 100 mg to maximize their accumulation in tumor tissues 3-6 times higher than in healthy tissues after 3-6 hours after administration, characterized in that, within 3 days before treatment, the person is transferred to a protein diet, for maximum selective accumulation, amplification and separation of the dielectric properties and electrical conductivity of "hungry" tumor and healthy cells with electron-ionic solutions of 3-aminophthalic acid hydrazide, and after 8 hours after taking the “Galavit” drug and subsequent AK administration 4 hours before the start of treatment, RF and microwave photoelectromagnetic hyperthermia of the tumor tissue with the energy of the photo-wave radiation is carried out, with the tumor tissue heating rate of 0.070 ° C / s, for 204 seconds to a temperature tumor tissue 51 ° C, when heating healthy tissue is not higher than 40 ° C.

One-time megadose of 150 mg of Galavit 3-aminophthalic acid hydrazide and 500 mg of ascorbic acid megadose is taken orally before the HF and microwave treatment of tumor tissues and heating and hyperthermia of tumor tissues saturated with a solution of Galavit 3-aminophthalic acid hydrazide and HF AK and microwave energy of photo-wave radiation, in accordance with the frequency, with the penetration depth of the electromagnetic wave and with the depth of the location of the tumor tissue, at the allowed frequencies f = 13.56 MHz-1100 cm; f = 27 MHz-545 cm; f = 40.68 MHz - 370 cm; f = 433.92 MHz - 34.5 s; f = 915 MHz - 16.5 cm and f = 2450 MHz - 6.1 cm.

According to studies on RF and microwave hyperthermia of tumor tissues, at a temperature of 45-60 ° C, the border between the necrosis zone and healthy tissue is several cells. The destruction zone of tumor tissue includes a small periphery of normal healthy tissues, which excludes the regenerating cells from metastasis by their secondary necrosis from tumor tissues.

The physical nature of microwave radiation is the physical field of moving electric charges in the electric and magnetic fields, which are a single electromagnetic field (EMF), characterized by the frequency of oscillation G. The difference is only in the frequency with which electromagnetic waves of the corresponding wavelength occur. The biological effect of EMF on a living organism consists in the absorption of energy by biological tissues, characterized by biophysical parameters - dielectric constant and conductivity.

The tissues of the human body, due to the high content of water in them, should be considered as lossy dielectrics. With general body irradiation, EMF energy penetrates to a depth of 0.5 wavelength. The intensity of exposure, exposure and dielectric loss and conductivity characterize the selective absorption of EMF by various tissues at the same EMF radiation density.

,

,

where λ is the wavelength

C is the propagation velocity of the electromagnetic wave,

f is the frequency of the electromagnetic field. The frequency with which oscillations of the electromagnetic field occur significantly affects the depth of penetration of the electromagnetic wave into a biological object (Table 1).

The reason is commensurability with various physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, the wavelength of the EMF λ = 33 cm, and at f = 2450 MHz, the wavelength of the EMF λ = 12.2 cm (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with 3-aminophthalic acid hydrazide are 6-8 times higher than its content in healthy tissues, and accordingly, its electrical conductivity is so many times different, i.e. the ability of tumor tissues to conduct an electric current is due to the presence of an acid electrolyte in the tumors, free charge carriers - electrically charged particles, which, under the influence of an external electric field in the thickness of the tumor, create a conduction current.

Another important parameter of dielectric and semiconductor materials such as tumors are dielectric losses. They serve to determine the electric power spent on heating dielectrics and semiconductors in an electromagnetic field. In the reference literature, for the characteristics of the ability of a dielectric to absorb the energy of an alternating electric field, it uses tanδ of the dielectric loss angle and permittivity ε. The physical meaning of tanδ is the presence of dielectric losses leading to a phase shift between the current and voltage, where the angle between them becomes less than 90 ° by an amount, the quantitative wave energy loss is proportional to the dielectric loss ε tanδ.

Losses on electrical conductivity in dielectrics having a low specific volume resistance, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with a changing wave electromagnetic field, converting electrical energy into heat. Tumor tissues are maximally saturated with 3-aminophthalic acid and AK hydroside, in this case they are semiconductors containing several times more charged ions in comparison with surrounding healthy tissues and, accordingly, their heating rate is many times higher than surrounding healthy tissues in one, and also time. In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of the polar water molecules in the direction of the electric field lines. Intramolecular friction occurs, which once again enhances the heating of tumor tissues.

The specific power of the dielectric loss, referred to the unit volume of the dielectric is called the dielectric loss, which can be calculated by the formula:

Ore = E ² fεtgδ, 10 ^-12 W / cm ³

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an electric field. For this, it is necessary to know ε and tanδ of tumor and healthy tissues, and thus very accurately calculate the heating rate to a given temperature of heating of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective absorption of 3-aminophthalic acid hydrazide and AK by the tumor tissues leads to their selective heating of the tumors and electromagnetic photoluminescence to a higher temperature of 51 ° C during heating of healthy tissues surrounding them to 40 ° C during the same time, which leads to inactivation of the tumor tissue and their subsequent destruction, which then, within a few days, are painlessly excreted by the body. The rate of heating by the wave energy of an electromagnetic field depends on the power of dielectric generators and magnetrons.

With an oscillatory power of electromagnetic field generators of 700-850 watts, 200-300 grams of tumor tissue can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors, and their heating temperature is determined by the formula:

where Co is the heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ is the temperature difference of the heating;

t is the heating time, s.

This formula allows you to select the required total specific power R _beats for RF and microwave heating of tumor tissues P _beats up to a given difference in heating temperatures and specific power R _beats. secreted in healthy tissues determined by the general formula:

R _{beats about.} = P _{beats op.} + P _{beats health}

Then the specific power in the irradiation region, taking into account the dielectric properties:

Knowing the dielectric properties of the tumor ε _op tgδ _op and healthy tissues ε _zd tgδ _zd , it is possible by calculation to determine the temperature of their heating ΔT to the required set temperatures and to determine the heating time t and the total specific power P _specific , irradiated area. (Table 2)

Similarly, knowing the dielectric parameters εtg δ and the specific density of tumor tissues in biological objects γ g / cm ³ , it is possible to calculate the specific power released in tumor tissues P _beats saturated with various electrophotosensitizers and determine the set temperature and calculate the time of their heating by HF and Microwave energy, according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells by RF and microwave energy, intended for local treatment of cancer patients with tumor tissues with metastases for their hyperthermia in the organs of the human body, characterized in that before treatment the person is transferred to a carbohydrate-free diet for 3 days to create glucose starvation and increased electrical conductivity and dielectric loss in “hungry” tumor cells compared with healthy ones saturated with an electron-ionic solution of 3-aminophthalic acid hydrazide when taken orally in a megadose of 151 mg in 8 hours and ascorbic acid in a megadose of 500 mg in 4 hours for them the maximum accumulation in tumor tissues is 3-6 times higher than in healthy tissues, selective hyperthermia of tumor tissues with microwave energy is carried out in accordance with the depth of penetration of an electromagnetic wave into the human body at 6.1 cm at allowed frequencies f = 2450 MHz with a total heating rate tumor tissues at these frequencies of 0.070 ° C / s for 204 s to a temperature of the tumor tissue of 51 ° C, with the heating of healthy tissues no higher than 40 ° C.