RU2724325C2 - Method for initiation of tumor cell death by folic acid and hf and uhf wave radiation energy - Google Patents

Abstract

FIELD: medicine; pharmaceuticals.SUBSTANCE: invention relates to a method for initiation of tumor cell death, intended for complex treatment of oncological patients having tumor tissues in all organs of human body, by hyperthermia HF and UHF energy, characterized by that person for 3 days is transferred to a non-carbohydrate diet to create glucose starvation and subsequent maximum saturation of oncological cells with electron-ionic solution of folic acid by oral administration in a disposable megadose of 31 mg per person with its accumulation in tumors is 3–6 times higher than in healthy ones, and after 4.5 hours, selective hyperthermia of tumor tissues HF energy in accordance with depth of their location and penetration depth of electromagnetic waves into human body 1100 cm, at the resolved frequency f=13.56 MHz, with a total heating rate of tumor tissues of 0.02 °C/s, for 420 s to tumor tissue temperature of 46 °C, with heating of healthy tissues not higher than 40 °C.EFFECT: said method results in thermal death of tumor tissues due to high dielectric contrast of the tumor and increasing the difference in its conductivity (dielectric loss) relative to surrounding healthy biological tissues, reaching multiple value for various tumors.1 cl, 2 tbl

Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects by folic acid and the energy of the wave RF and microwave radiation, known as RF and microwave hyperthermia.

Hyperthermia is called in medicine, as a significant increase in human body temperature over 40 ° C. Hyperthermia for cancer treatment was used half a century ago. The German doctor von Ardenne opened a "thermal" clinic in a water bath for hopelessly oncologically ill patients, whom he heated to 42 ° C. After this procedure, no more than 17% of people survived, but they were completely cured. The rest were dying, not withstanding such a high temperature. This technology is now used in the United States, where the human body is heated to 42.5 ° C, followed by its return to life. This treatment technology can be effectively used for the selective heating of oncological tissues with RF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

The method for initiating the death of tumor cells by electromagnetic energy of the wave RF and microwave radiation consists in the complex simultaneous effect of folic acid and wave irradiation of the electromagnetic energy of the RF and microwave on the tumor tissue. After oral administration of a solution of folic acid inside a person, for its maximum accumulation and preservation in a significant amount in the tumor tissues of the body, it takes time for 3.5-4.5 hours before treatment, the cancer patient must take a single dose of one of the above drugs until 15 mg and 3-6 hours after administration, i.e. the maximum accumulation of folic acid in tumor tissues, to conduct RF and microwave hyperthermia of tumor cells with high-frequency radiation energy, with a heating rate of 0.4-0.1 ° C / s to a temperature of 46 ° C, as a result of this process, the tumors denature and subsequently decay products tumor cells are excreted by the body independently, naturally, excluding surgical intervention in the human body. The maximum content of folic acid in tumor tissues occurs precisely at this period of time and it is several times higher in tumor tissues than in healthy ones due to the selective absorption of folic acid by tumor tissues.

A known complex of folic acid or a folic acid derivative consisting of folinic acid, dehydrofolic acid, tetrahydrofolic acid, tetrahydropterin, pteroyl-polyglutamic acid, etc., and polysaccharides consisting of glucans, dextrans, polysaccharose, fructosano hetero, - or hetero-polysaccharides, polysaccharide of ginseng pictin, etc. These complexes of folic acid or its derivative penetrate the cell along a pathway involving folic acid receptors on the cell membrane, due to the polysaccharides taken by oncocytes as glucose. This complex of folic acid or its derivative is used to obtain drugs with antitumor activity.

Folic acid tablets are available to replenish vitamin B 9 deficiency, folacin preparations, in one tablet 5 mg of vitamin B9, Alo-folica, in one tablet 5 mg of vitamin B9, folio, in one tablet 0.4 mg of vitamin B9 and 0.2 mg of iodine , Materna, in one tablet 1 mg of vitamin B9, Elevit, in one tablet 1 mg of vitamin B9, Vitrum Prenatal, in one tablet 0.8 mg of vitamin B9, Multi-tabs perinatal, in one tablet 0.4 mg of vitamin B9, pregnat , in one tablet 0.75 mg of vitamin B9 An overdose of folic acid is very rare and was observed when taking drugs in the amount of 20-30 mg of vitamin B9. With a slight excess of the required amount of the drug, an excess of folic acid is excreted without causing any harm to healthy body tissues.

Folic acid (also known as folate, vitamin M, vitamin B9, vitamin Bc (or foliacin), pteroyl-L-hylutamic acid and pteroyl-L-glutamate) is a form of water-soluble vitamin B9. Folic acid consists of an aromatic pteridine ring bound to para-aminobenzoic acid and one or more glutamate residues. Folic acid in itself does not exhibit biological activity, its biological significance is manifested due to tetrahydrofolate and other derivatives after its conversion in the liver to dehydrofolic acid, which then enters the human circulatory system and tumor tissues, which accumulate it in large quantities. Vitamin B9 (folic acid and folate) plays many roles in the human body. The body does not produce it, but only comes with food and is important for the rapid division and growth of cells, especially at a young age, including the rapid growth and reproduction of cancer cells and their selective absorption of folic acid. Studies by scientists from St. Michael's Hospital (Toronto) for the first time showed that consumption of folic acid, at a dosage of 2.5 mg five times a day, significantly contributes to the growth of existing precancerous or cancerous cells in the mammary glands of mice. Conducting new studies on the short-term use of folic acid does not affect the risk of developing any particular type of cancer of the prostate, colon, lung, or breast (published in The Lancet). According to the researchers, it is argued that taking folic acid as a medicine or dietary supplement is actually safe. Subsequent studies published in CANCER magazine have shown that folic acid supplementation can stop cancer progression and contribute to disease regression.

Folic acid is activated in the human liver into tetrahydrofolic acid, which is involved in the synthesis of pyrimidine and purine base components of nucleic acids (DNA, RNA). It takes part in the exchange of amino acids (glycine, methionine, etc.). Stimulates erythropoiesis, improves iron absorption.

Recommended for the treatment of cancer, dextran folic acid complexes, which are effectively absorbed by the surfaces of tumor cells, to increase the volume of blood in tumor cells. Folic acid polysaccharide complexes are able to penetrate into tumor cells through cell membranes and are able to destroy metachondria of tumor cells and inhibit the growth of tumor tissue without damaging normal cells. Folic acid polysaccharide complex is a salt applicable in medicine. If an alkaline nitrogen atom is present in the molecule of the complex under consideration, then inorganic or organic acids are formed: hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid propionic acid, benzenesulfonic acid, etc. This complex can also form salts of sodium pyraxide, sodium hydroxide, potassium hydroxide, omonium hydroxide, triethylamine, ethanolamine, demethylaminopyridine. Preferably, the carboxyl group of folic acid and the hydroxyl group of polysaccharides are used.

For treatment, tablets of Folacin and Foliber drugs and B vitamins are used. The additional use of folic acid folates actively breaks down the internal fat reserve of cells and breaks down into simple alcohols, which are then excreted through the human excretory system.

The proposed complex of dehydrofolic acid obtained as a result of FC oxidation of the preparations Folacin and Foliber, is oxidized with hydrogen peroxide in an alkaline medium, the reaction is catalyzed by heme of iron, and causes chemiluminescence under the influence of photo-wave RF and microwave hyperthermia, with the active release of singlet oxygen. If an oxidizing agent, lipo folic acid peroxide, is added to an alkaline solution of oncocytes, then a glow occurs. In the presence of catalysts, this glow increases and becomes brighter. The role of catalysts for the solution of Dehydrofolate acids obtained as a result of oxidation of FA is carried out by hemin of blood iron and various sodium compounds during high-frequency and microwave heating. These chemical activators of chemiluminescence enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. Observed during this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is dehydrofolic acid, obtained as a result of the oxidation of FA in the presence of oxygen radicals. Under the action of the FC oxidizing agent - radicals, the formation occurs, which reacts with the superoxide radical, which forms the internal peroxide (dioxide), which leads to the formation of excited molecules of folic acid preparations. The transition of these molecules to the initial ground state is accompanied by the emission of a quantum of light upon high-frequency heating. Hydrogen peroxide is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, it is a relatively harmless compound, but in the presence of metal ions of variable valence of iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide H ₂ 0 ₂ a destructive hydroxyl radical is formed JOH, which causes mutations and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl group of enzymes activates molecules under the influence of high-frequency energy and actively enters into a chemical reaction with them, which leads to a bright glow of tumor tissues.

Some cells of the body, granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells in the presence of reactive oxygen species contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and hydroxyl radical JOH in this case there is weak chemiluminescence, which is amplified repeatedly in the presence of FC with RF and microwave irradiation. These effects are also amplified many times by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the cell metachondria.

When placed in an alternating electromagnetic field of high tension and frequency of various biological bodies, they begin to emit a characteristic radiance of various intensities and colors, from which one can judge the properties of the studied object. The method of “high-frequency photography” (the Kirlian effect, kirlianography in honor of the inventor V.K. Kirlian) is now widely known in Russia and abroad as a method of experimental studies of electromagnetic fields and bioenergetic interactions. But the greatest scientific and practical interest is the study of the glow of biological objects in an alternating electromagnetic field of high frequency, explained by the photoelectromagnetic effect of photo-wave radiation and luminescent biological objects.

In accordance with modern concepts, aqueous solutions of alkalis and acids in the human body are considered as an associated liquid consisting of separate associated elements - neutral clusters and cluster ions of the general formula (Н ₂ O) _n , [(Н ₂ 0) _n ] ⁺ , [( H ₂ ) _n ] ^- , [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ], etc. d. where the number of water molecules bonded into hydrogen bonds can n times reach, according to some authors, hundreds or even thousands of units. Changing the position of one structural element (water molecule) under the influence of any external factor or changing the orientation of surrounding neighboring water molecules provides high sensitivity of the entire water information system to various external influences (electromagnetic, thermal, sound fields, bio-exposure, etc.).

In addition, in water clusters due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, proton (H ⁺ ) migration can occur via the relay mechanism, leading to proton delocalization within the cluster. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-information, etc. In addition, aqueous solutions, various chemical elements, is a source of super-weak and weak alternating electromagnetic radiation. In this case, the induction of the corresponding electromagnetic field and resonance effects of combining (superposition) of electromagnetic fields that can change the structural and informational characteristics of biological objects, 80-90% consisting of water with various chemical impurities, can occur.

Under the influence of a high frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, the N ₂ , H ₂ , O ₂ and CO ₂ molecules are excited, polarized and ionized. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of the object, can color the luminous corona in various colors. The shape of the glow corona, its density, brightness, and surface distribution are determined mainly by the photoelectromagnetic parameters of the object.

These effects are also amplified many times by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the cell metachondria. This effect of the influence of electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nicolo Tesla, when exposed to pulsed high-frequency energy of vessels with liquids with the ability to emit light and fluorescent lamps that glowed without connecting to electric wires, with bright light in the hands of Nikola Tesla, with whom he also juggled, which caused genuine delight among the audience, while at the same time a phenomenon inexplicable by nature, which only Nicolo Teslo knew. These factors in biology are called collective stimuli of luminescence that alter the state of blood and tissue phagocytes and their ability to increase the excretion of reactive oxygen species, and accordingly the protective functions of cells.

It can be argued that there would be even fewer side effects, and the effect would be greater if treatment with powerful doses of FC were the basis against the background of complete overlap of the intake of carbohydrates - glucose, as competitors of FC in oncocytes. For this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract turn into glucose, which is essential for the nutrition of cancer cells. With this introduction of cancer cells into artificial glucose "starvation," then a person needs to enter high single doses of FC (vitamin B), better than its complex of lyophized powder, dry syrup of the intestinal capsule and other drugs in the form of tablets of Antifirin, Compositon, Falacin, Fomibier, etc. The required amount of the drug based on the maximum permissible single dose of folic acid not exceeding 20-30 mg, because folic acid is more toxic than other B vitamins (riboflavin, pyridoxine, etc.) Under the action of enzymes in the human body, folic acid is converted to folinic acid, which is 100 times more active than folic acid. A lot of folic acid is found in foods. When it enters the blood vessels of the tumor, which have a large branched network with thin peripheral vessels and a low speed of movement in the blood in them, the “hungry” tumor is maximally saturated with folic acid polysaccharide, several times higher than in normal healthy tissues, it stimulates the formation of hydrogen lipoperoxide and alkali nitrogen, in a sufficiently large amount on the membranes and interstitial fluid. It is this chemical compound that is formed during the interaction of vitamin B and the internal environment of the body. Lipoperoxide is a factor or hormone that stimulates the mechanisms of self-destruction and the destruction of cancer cells. The formation of sufficient doses of lipoperoxides around and inside the cancer cells and their apoptosis is possible only with a sufficiently large amount of folic acid intake. Under these conditions, the polysaccharide FC can manifest itself as an antioxidant or prooxidant, i.e. an oxidizing agent, including the destructive, rather than creative, property of cancer cells. This is very important in cell energy. Therefore, FC can be described as a metabolic switch that accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in the mitochondria and is replaced by glycolysis. When entering the cancer cell, FC inhibits glycolysis, but is not able to transfer it to the path of normal aerobicity. Perhaps this is due to the competitive presence of glucose. To completely disable glycolysis in tumor cells, it is necessary to completely exclude the access of glucose, or so that FC over glucose prevails in the substrate. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In cancer cells, when there is an excess of it, it stimulates the processes of oxidation, with the formation of alkali nitrogen and lipoperoxides, which, when they are excess, have a toxic effect on cancer cells.

Folic acid is actively imported into endoplasmic reticules (EPR) (an endoplasmic reticulum consisting of membranes and setting the direction and active transport of substrates against gradients) of cells using polysaccharide transporters. It should be noted that the energy processes in cancer cells are transferred from metachondria to the endoplasmic reticulum. It is here that FC accumulates in the EPR and the cancer cell environment in this place is significantly different from ordinary cells, it is simply re-restored here, and here folinic acid is obviously forced to recover to FC. From this moment, the destructive effect of FC on the cell begins. A “hungry” cancer cell at this time can repeatedly accumulate FC, as perceives it on its membrane transporters for glucose. Since there are many times more glucose-consuming receptors in the oncollet than in healthy ones, although the transport systems for delivering glucose and FC to the cell are common, this is the Trojan horse for oncocytes. Thus, it is very easy to deceive oncocytes and pump FC into them, with the solution of the problem of feeding megadoses of FC, and then the phenomenon of death of oncocytes will be strengthened many times.

A "hungry" tumor is maximally saturated with folic acid, 6-8 times higher than in normal healthy tissues, in a fairly large amount on membranes and interstitial fluid. A “hungry” tumor in the absence of glycolysis saturates for a maximum of 3.5–4.5 hours, and is many times higher than in normal healthy tissues, it stimulates the formation of macrophages and T lymphocytes under the action of the enzyme ferrachelase, in a sufficiently large amount on membranes and interstitial fluid. It is this chemical compound that is formed in the process of the interaction of FC and hydrogen peroxide in the internal environment of the body. Under the action of the oxidizing agent of lipoperoxides and the formation of hydrogen, significantly enhanced temperature and the action and additional photodynamic action, electromagnetic fields of HF and microwave, active hydrogen and oxygen radicals are formed, which then reacts with superoxide radicals that accelerate and form internal peroxide (dioxide), H ₂ O ₂ with their hyperthermic decomposition, RF and microwave energy of the FC. In this case, there is a multiple amplification in the formation of excited oxygen molecules. The transition of FC molecules and internal hydrogen peroxide from the excited to the ground state is accompanied by the emission of light quanta and strong luminescence. As a result of these chemical reactions associated with a high release of active forms of hydrogen and oxygen and organic free radicals, cancer cells are burned.

The method of "selective starvation" of cancer cells superficial and deeply located in the human body, by subsequent administration or administration of various sensitizers, for the selective maximum saturation of tumor cells with highly electroconductive electron-ion solutions of electrophotosensitizers with the maximum separation of electrophysical properties, tumor and healthy tissues, followed by selective exposure to them high-frequency electromagnetic fields in combination with other methods - this is the most relevant scientific and practical direction in the fight against cancer.

A number of researchers argue that the minimum molar concentration of polysaccharides of vitamin B vitamin F, which is a prooxidant (lipid-oxidizing compounds that neutralize free radicals), kill cancer cells in the blood and tissues without affecting healthy ones, due to the local oxidative stress-damage process caused by oxidation of the cell DNA and depletion of adenosine triphosphate (ATP) is a cell energy source. Alkalis of nitrogen and lipoperoxide, among other molecules accompanying it, aggressive action, causes a malfunction in the functioning of a certain enzyme responsible for the "nutrition" of cancer cells. FC is a representative of intermediate acids that can accumulate in the cytosol of cells.

The study of biophysical and biochemical mechanisms determines two concepts of the death of cancer cells, one suggests the importance of FC, the other the importance of RF and microwave hyperthermia. The general combination of these methods will lead to the apparent death of cancer cells. The main task for researchers is to maximize the effect of the maximum selective absorption of trisulfophthalocyanine hydroxyaluminium by cancer cells with the subsequent subsequent high-frequency irradiation of cancer cells in order to increase the effectiveness of treatment to 100%.

It has already been proved that such an effect is possible by the example of disinfection of biological objects from viral, fungal and bacterial infections with HF and microwave energy. Numerous studies conducted by us in the Krasnoyarsk State Agrarian University and VIZR in St. Petersburg have confirmed the 100% effectiveness of disinfecting vegetable seeds and living bioobjects saturated highly conductive electron-ionic solutions of micronutrients of high frequency and microwave energy against viral infections that have a similar origin with cancer cells. The data are presented in the works:

A.S. No. 563938 of the USSR. A method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bull. No. 25. USSR Certificate No. 950214. The method of presowing treatment of seeds / Tsuglenok N.V. - Registered in the register 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with energy of high-frequency and microwave: methodical recommendations / N.V. Loam. - M .: Agropromizdat, 1989. Guidelines for the use of HF and microwave energy in the processes of preparing seeds for sowing / N.V. Loam. - M.: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfecting tomato seeds against a viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute for Plant Protection (VIZR). - 1989. -T. 71. - S. 49-54. The method of disinfection of egg powder. Patent Number: 1734632. Published: May 23, 1992 Authors: Tsuglenok N.V., Kolmakov Yu.V. IPC: A23v 5/02. Method for preparing medium for diluting sperm of a manufacturer Patent number: 1769422. Published: June 27, 1995. Authors: Tsuglenok, Ostashko, Chess, Silantyev, Kontsedal.

It is proved that oncoviruses under the influence of carcinogens integrate into a healthy cell and dissolve in it over time, turning it into a cancer cell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless, they simply are not there. Of particular note is the new photodynamic method of using laser photosensitizers. But the small penetration depth of the electromagnetic wave of laser emitters does not allow to burn deep malignant tumors.

It is worth noting another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein shell filled with a mixture of nucleic acids and similarly tumor cells filled with intercellular fluid solutions determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. HF and microwave heating in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity and dielectric loss, which further enhances their selective heating and apoptosis of viruses and tumor tissues.

It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects.

Tumor cells, when saturated with PK, accumulate, in contrast to normal cells, a significant amount of theolactone homocysteine (HTL). Prior to insertion of homocysteine into the protein, it becomes a biological defect in the form of (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone practically does not form from it, but conversion to a cancer cell requires significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein synthesizing the cancer cell machine works at full capacity, therefore it is often mistaken. When a folic acid polysaccharide complex enters an HTL-saturated cancer cell, 3-mercaptopropionic aldehyde is formed, which, when irradiated with HF and microwave energy, kills cancer cells. By destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer much from it. In this case, it can be argued that in the treatment of cancer with folinic acid obtained by the oxidation of D-FC RF and microwave energy, both in blood and in human tumor tissues, a 100% therapeutic effect is observed.

This healing effect is explained by the fact that during 3.5-4.5 hours in normal cells of living biological objects, the folic acid polysaccharide complex quickly turns into folic acid and divalent iron heme, sodium hydroxide, keel hydroxide, homonium hydroxide under the action of the enzyme ferrochelatase, while maintaining a high contrast of the content of folic acid and its conversion into folinic acid into tumors, which significantly increases its concentration and, accordingly, electrical conductivity with a significant change in the dielectric properties of tumors relative to surrounding healthy biological tissues.

With further hyperthermia of tumor cells saturated with folic acid, for 420 sec. with a heating rate of 0.02 ° C / s to a temperature of 46 ° C by the wave radiation of the HF and microwave fields, with a permitted frequency of oscillations of the electromagnetic field f = 433 92 MHz, f = 915 MHz or 2450 MHz, This allows simultaneous conducting and conducting and exposure folinic acid and HF and microwave hyperthermia of tumor cells with wave radiation energy destroying them, even undetectable tumor formations located in the deep layers of a biological object.

The biophysical meaning of this method consists in the selective maximum saturation and accumulation of highly electroconducting electron-ionic solutions of electrophotosensitizers in tumor cells and in the maximum separation of electrophysical properties of tumor and healthy tissues with folinic acid and a significant increase in the difference in electrical potentials of tumor and healthy cells in the intercellular medium and on the walls of the reticular medium . Reticulum is an electrical circuit where negative charges are evident on one side of the membrane and positive charges on the opposite side, so the reticulum is an electric transporter of glucose and other nutrients to cancerous and healthy cells. Therefore, the reticulum is an electric network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of metachondria and energy operator structures on the outer side of the cell - on cilia. Under certain situations in the environment of the cell, these proteins, when discharged, can give an active signal to the reticulum and metachondria. This changes the balance of the existing charges on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are launched. One side of the reticulum membrane is connected to one type of entrance to the metachondria, and the opposite to the exit from it. Thus, a single electric circuit of double active energy management of metachondria is created. The electric field strength on the reticulum keeps under control the work of metachondria. In this case, the metachondria pull in the charges accumulated on one side of the reticulum membrane and remove the opposite charges on the other side of the reticulum membrane. The charges, therefore, do not mix and separate. This is important so that the cells undergo ion exchange. Outwardly, the reticulum is similar to the capacitor plates, the more layers of the plates, the greater its electrical intensity. Between the gaskets is a semiconductor saturated with a folic acid polysaccharide complex. This capacitor, i.e. a powerful dense network of tumor membrane membranes is very clearly visible through a microscope. In tumor cells, the number of membranes is much higher than in healthy cells. Accordingly, the density of the tumor tissue and the capacity of the biological electrical capacitor are significantly higher than healthy tissues. When charging such a capacitor on one plate, negatively charged electron particles will collect, and on the other - ions, positively charged particles. Such a charged capacitor can turn into a current source if it is turned off. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which discharges the charge on the metachondria, controlling their activity. Metachondria, in turn, are configured so that they never allow the charges to reticulum to drop below a critical level. In oncological cells, the charges inside the metachodria sharply decrease and the entire regulatory system is disrupted. This is the main core of the control of the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not basic. As a result of the electrochemical energy of the cell in the reticulum, there is a cycle of substances where the metachondria are the pump. With a lack of this cycle between the reticuli and metachondria due to electroosmosis, there is a suction of substances from outside through the outer membrane and the opening of locks and a polysaccharide pump on it. The medium on the membranes of the reticulum and the liquid substrate is re-restored, due to the excess of minus charges. This determines the chemical equilibrium in pH of conjugated buffer electro-chemical substances when the buffer system is discharged or restored. The charges on the plates of the reticulum and metachondria regulate these processes. Chemical processes, in this case, are simply performers, intermediaries. The outer side of the metachondria provides the voltage of the dependent anion channel. This voltage maintenance mechanism, called VDAC, sets the conditions for the reticulum to function. It is here that on the outer side of the membrane is the enzyme Hexokinase II, which ensures the utilization of glucose. Separation, disconnection of the outer mitochondrial membrane (VDAC) and Hexokinase II provides the induction of apoptosis.

Metachondria works by pulling from the reticulum into itself as an electromagnetic pump, the necessary power under high voltage. Without this effect of the highest tension, pulling in nutrients into the cell will not happen. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Oncocytes, unlike normal cells, do not have cilia. This, the most striking, energy level in cancer cells is absent. Therefore, the only right way to find a weak spot in the energy of cancer cells and thereby destroy them. Metachondria set the degree of charge to ion pumps on the outer membrane of the cell and starter structures that hold charges on the reticulum. These sensory structures can most quickly be damaged and burn out, since metachodria are the most effective electrochemical furnaces. If metachondria is switched off, the voltage gradient of the cell decreases sharply and the processes go on in the cells on a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. A cancer cell does not take quality, since everything is concentrated on a small area of metochondria, but with a larger quantity, much larger than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but at the same time it requires glucose, which, in this case, uses the folic acid polysaccharide complex to feed “hungry” cancer cells.

The membranes of the reticulum and the nucleus of the cell are the same, and the reticulum, as a capacitor, is contoured to the nucleus by only one of its electron sides and dumps electrons into the nucleus. Thus, the reticulum charge also provides a charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a heavy-duty electrostatic charge inside the nucleus.

In cancer cells, in comparison with healthy ones, the medium is different, converted to folic acid, which tries to burn and destroy everything in the cancer cell to the maximum due to lipid peroxidation (LPO) during RF and microwave heating. In this case, significant damage occurs with the formation of toxic lipid peroxides, damage to cell membranes, various organs, mutation of nucleic acids, inoculation of enzymes, destruction of nutrients and cell death. In this case, cell death does not follow the path of apoptosis, but the path of maximum necrosis, due to chemical activators that react with reactive oxygen species or free radicals released during the main process of RF and microwave hyperthermia. In this case, the formation of products of an excited electronic state produces the emission of photons. The study of biological objects shows their connection with free radical oxidation in the body, proceeding without the participation of enzymes, due to the reduction of molecular oxygen to its active forms: superoxide onionradical, hydroxyl radical, singlet oxygen. The main source of these compounds is the autooxidation of lipids, mainly unsaturated fatty acids (folic), in which free radicals are released under the influence of temperature and the rf and microwave fields that interact with oxygen to form peroxides. Upon recombination of these perxides, quanta of light and singlet oxygen are released, which destroy tumor cells. A similar fact was established by researchers under the action of short-term electrical impulses that stimulate the release of reactive oxygen species and increase the permeability of cell membranes. These effects of lipid peroxidation are observed in tumor tissues with a prolonged lack of oxygen.

Folic acid has acceptor properties with respect to hydrogen, which are present in excess in cancer cells and this determines its participation in redox processes. Under the influence of temperature, folic acid is metabolized to tetrahydrofolic acid, which is a factor in enzymatic systems involved in the transfer of various carbon radicals. Folate coenzymes are involved in the biosynthesis of purine and pyrimidine bases, nucleic acids, amino acids, and also increases the use of glutamic acid and tyrosine by tumor tissues.

The more folic acid in the cancer cell, the more peroxidized lipids and lipoperoxides are formed there, in comparison with healthy cells. An excess of peroxides triggers the death of cancer cells in the RF and microwave fields by rapidly heating the cells to 46 ° C. Due to the rapid oxidative decomposition of folic acid and oxidized lipids under the influence of temperature and the RF and microwave fields, they interact with free oxygen to form peroxides. Upon recombination of these perxides, quanta of light and singlet oxygen are destroyed, which destroy tumor cells.

The most complete accumulation of folic acid in the tumor occurs within 3.5-4.5 hours after ingestion. Then the level of folic acid in the tumor gradually decreases, reaching its initial values 24-48 hours after taking the drug.

There are a number of folic acid biosynthesis drugs: trimethoprine, pyrimethamine, methotrixate and sulfanamides 4-aminobenzoic acid inhibitors.

A known method for the production of a folic acid polysaccharide complex, which is a denitropholic acid polysaccharide, allows penetrating into a folic acid cell along a pathway involving glucan receptors and other hydrophilic alcohol polymers of folic acid on a cell membrane as antitumor preparations. (RF patent No. 2280650 - LU Weiyu (CN) LIU Min (CN) PAN Yun (CN)). This complex has antitumor activity, provides long-term treatment and does not guarantee 100% cure, since folic acid without a transporter polysaccharide is also actively involved in the nutrition and active division of cancer cells.

A known method of destruction of cancer cells during microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the resonance frequency of the absorption of the tumors is detected. Then they carry out a similar effect on healthy tissues bordering on the tumor and reveal the value of the resonance frequency of absorption of these healthy tissues. Simultaneously with hyperthermia, the values of the resonant frequencies are monitored by the absorption of energy by tumors and healthy tissues, and when the values of the resonant frequencies come closer together, the energy absorption by tumors and healthy tissues is judged on the effectiveness of the treatment. This method allows to increase the effectiveness of the treatment of tumors by microwave hypothermia when they are heated to 43 ° C.

The main disadvantage of this method is the small penetration depth into biological tissue, which is several millimeters, due to the strong absorption of microwave radiation, with a wavelength of 1.3-2 cm, water molecules and a small difference in the heating of tumor and healthy tissues.

A known method of destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6) the essence of the invention includes the introduction into the tumor localization region of ferromagnetic particles, followed by local induction heating, in the temperature range from 42 ° C to 45 ° C, over time, determined by the type of tumor, its size, localization and type of ferromagnetic particles selected for induction heating, while heating is carried out only at times when the blood supply to the tissue decreases, i.e. during exhalation and diastopes of the patient’s heart. The heating range is controlled by a microwave depth thermometer, and heating is carried out automatically, using a computer, in biocontrol mode, according to the algorithms of a mathematical model of fluctuations in the thermal conductivity and heat capacity of the fabric, hysteresis of heating and heat removal.

The main disadvantages of this method is the small localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in various spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, Serdar Deger, Stefan Loening, Wolfgang Lanksch, Roland Felix Journal of Magnetism and Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on the thermolysis of magnetic nanoparticles introduced into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions across cell membranes, as well as induce inducing alternating electric fields that affect the functioning of neural networks in the human body, associated with heating not only magnetic particles, but also all cells, located in the area of introduction of magnetic particles, and a strong spatial heterogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength that matches the absorption band of the photosensitizer (see Photodynamic therapy / Ed.TJ Dougherty / J. Clin. Laser Med Surg. 1996, Vol. 14, P 219-348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of photosensitizer accumulation in tumor cells compared to healthy cells, which is associated with a higher density of blood vessels in the tumor compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, the dye molecules transform into an excited electronic state and, when they collide with oxygen molecules dissolved in biological tissues, transfer it from an unexcited to an excited electronic singlet state with a typical lifetime of several microseconds. During this time, the molecules of singlet oxygen, having passed a characteristic path commensurate with the size of the cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, cell destruction occurs only during exposure to laser radiation in the spatial region of laser beam irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. Phthalacyin photosensitizers used in practice. porphyrins, chlorins have absorption bands of photosensitizers in the ultraviolet or visible spectral range, and the used lasers cannot effectively penetrate to a depth not exceeding a few millimeters. In addition, the photodynamic method has a low contrast accumulation of photosensitizers in cancer cells.

A known method of short-focus x-ray therapy with a total focal zone of 100-120 Gy and remote gamma therapy for radiation destruction of malignant cells with a total focal zone of 30-40 Gy (see Sh. H. Gantsev. Oncology, M.: Medical Information Agency. 2004, pp. 190-204; Stephen J., Withrow E., MacEwen G. Smal animal clinical oncology - 2001, p. 305-308).

However, this method, despite the prevalence, has the following disadvantages. In the treatment of certain types of malignant neoplasms, for example melanoma, with the help of remote gamma therapy, even in combination with immunotherapy, as experience shows, leads to a 75-90% tumor recurrence, and metastases occur after 2-6 months.

Closest to the claimed one is a neuron - a capture method for the selective destruction of melanoma (see V.N. Mitin, N.G. Kozlovskaya, AM Arnopolskaya Neuron-capture therapy of oral tumors in dogs. All-Russian Veterinary Journal. 2006. No. 1, p. . 9-10).

The method involves intravenous injection of L-borphenylalanine, which selectively accumulates in a specific tumor - melanoma, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, selective accumulation of L-borphenylalanine in melanoma cells occurs. Upon irradiation of a spatial zone commensurate with a tumor containing L-borphenylalanine by a beam of slow neurons obtained from a neuron guide from a nuclear reactor, melanoma cells are destroyed due to the induced secondary local radiation of boron.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced when using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to serve.

3. Long-term patient exposure for an hour when monitoring the cardiovascular system.

4. The use of general anesthesia.

The objective of the present invention is the local selective destruction of malignant tumors deeply located in human biological tissues, pre-selectively saturated with folic acid for 4.5 hours, while selective high-frequency and microwave heating of tumors saturated with folic acid, heated to a temperature of 46 ° C at a speed heating of 0.02 ° C / s for 420 sec. and with secondary reverse heating of the boundary layers of healthy tissues due to thermal conductivity from tumor tissues, after switching off the HF and microwave energy supply, from the boundary layers of tumor tissues, due to contact heat transfer of high temperature, to healthy tissues, with minimal destruction of the surrounding healthy biological tissue cells, ready for degeneration into cancer cells.

The physical nature of microwave radiation is the same as that of light radiation. The only difference is in the frequency with which electromagnetic oscillations occur at the corresponding wavelength, which is the same because the latter is related to the frequency by the ratio:

where, λ is the wavelength;

c is the propagation velocity of the electromagnetic wave;

f is the frequency of the electromagnetic field.

The frequency with which oscillations of the electromagnetic field occur significantly affects the penetration depth of the electromagnetic wave into a biological object (Table 1).

The reason is commensurability with various physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, the wavelength of the EMF λ = 33 cm, and at f = 2450 MHz, the wavelength of the EMF λ = 12.2 cm (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with folic acid are several times higher than its content in healthy tissues, respectively, the conductivity of the tumor is so many times different, i.e. the ability of tumor tissues to conduct electric current and absorb the energy of an electromagnetic field. In the presence of an acid electrolyte in the tumors, free positively charged ions, which, under the influence of an external electric field in the mitochondria of the tumor, create a conduction current and bias currents in negative charges on the reticulums of the cell membranes, i.e. shells of cancer cells and in their nuclei.

Another important parameter of dielectric and semiconductor materials, which are tumors, is the dielectric loss of water, which is more than healthy in tumor tissues and it determines the loss of electrical power spent on heating tumor tissues that have dielectric and semiconductor properties in the electromagnetic field of the RF and microwave. In the reference literature, tgδ uses the dielectric loss angle and dielectric constant C to characterize the ability of a dielectric to absorb ac electric field energy. The physical meaning of tgδ is the presence of dielectric losses leading to a phase shift between current and voltage where the angle between them becomes less than 90 ° by the amount of quantitative loss wave energy are proportional to the dielectric loss εtgδ.

Losses on electrical conductivity in dielectrics having a low specific volume resistance, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with a changing wave electromagnetic field, converting electrical energy into heat. Tumor tissues in this case are semiconductors containing several times more charged ions in comparison with surrounding healthy tissues and, accordingly, their heating rate is several times higher than that of surrounding healthy tissues in one and the same time in a uniform electromagnetic field of HF and microwave. In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of the polar water molecules in the direction of the electric field lines. Intramolecular friction occurs, which once again enhances the heating of tumor tissues.

The specific power of dielectric loss, referred to the unit volume of the tumor, can be calculated by the formula:

Ore = E ² fεtgδ, 10 ¹² W / cm ³

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an alternating and electromagnetic field. For this, it is necessary to know ε and tanδ of tumor and healthy tissues, and thus very accurately calculate the heating rate to a given temperature of heating of tumor and surrounding healthy tissues.

The selective absorption of folic acid by tumor tissues and the simultaneous exposure to HF and microwave energy leads to the selective heating of tumors to a temperature of 46 ° C in 420 sec, while heating healthy tissues surrounding them to a temperature of 40 ° C during this time. When tumors are heated at 46 ° C, ascorbic acid decomposes into peroxide, with the release of a hydroxyl radical, singlet oxygen, and light quanta are released. The sources of chemiluminescence and free radical oxidation are excited molecules of oxygen dimers, ketones, cyclic hydroperoxide, oxolates and aldehytes, biogenic amines. The same emission of fatons is observed during the decomposition of all peroxides (intermediate products of reactions with molecular oxygen). Free radical oxidation in the body is inhibited due to natural antioxidants, which are divided into hydrophobic antioxidants (tocopherols, flavins, carotenoids, steroids) and hydrophilic (sulfhydryl compounds of the SH-group of proteins, ascorbic acid) and phases. Thus, chemiluminescent activity can indicate excessive free radical oxidation in the body, under the influence of the energy of the currents of high HDTV and microwave energy. Vitamin B and a folic acid derivative in this case are very strong antioxidants, under the action of short-term electrical impulses that stimulate the release of reactive oxygen species and increase the permeability of cell membranes, which leads to the inactivation of tumor tissues and their subsequent destruction, which then, within 2-4 weeks, painlessly excreted by the body. The rate of heating by the wave energy of an electromagnetic field depends on the power of dielectric generators and magnetrons.

Knowing the specific power of Ore released in a biological object taking into account ε and tanδ of tumor and healthy tissues, it is possible to very accurately calculate the heating rate to a given temperature of heating of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF). according to the formula:

Ore _op = E ² fεoptgopδ, 10 ^-12 W / cm ³

With an oscillatory power of electromagnetic field generators of 700-850 watts, 200-300 grams of tumor tissue can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors, and their heating temperature is determined by the formula:

where, Co is the heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ-difference of heating temperatures;

t is the heating time, sec.

This formula allows you to select the required specific power Rud about for HF and microwave to heat the tumor tissues of Rud op to a given temperature difference of heating and the specific power Rud secreted in healthy tissues is determined by the general formula:

Ore about = Ore op + Ore zd

Then the specific power in the irradiation region, taking into account the dielectric properties:

Rood on = (E ² + E ² fεoptgδop fεzdtgδzd) 10 ^'12

Knowing the dielectric properties of the tumor εoptgδop and healthy tissues εzdtgδzd, it is possible to calculate by calculating their heating temperature ΔT to the required set temperatures and determine the heating time t and the total specific power Rud about the irradiated area. (Table 2)

Similarly, knowing the dielectric parameters εtgδ and the specific density of tumor tissues in biological objects γ g / cm ³ , it is possible to calculate by calculating the specific power released in the tumor tissues Rud op saturated with various electrophotosensitizers and determine the set temperature and calculate the time of their heating by HF and microwave energy according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells, intended for the complex treatment of cancer patients with tumor tissues in all organs of the human body by hyperthermia with RF and microwave energy, characterized in that a person is transferred to a carbohydrate-free diet for 3 days to create glucose starvation and subsequent maximum saturation of cancer cells with an electron-ionic solution of folic acid by oral administration in a single dose of 31 mg per person with its accumulation in tumors is 3-6 times higher than in healthy ones, and after 4.5 hours, selective hyperthermia of tumor tissues with RF energy is performed in accordance with the depth of their location and the depth of penetration of the electromagnetic wave into the human body 1100 cm, at an allowed frequency f = 13.56 MHz, with a total heating rate of the tumor tissue of 0.02 ° C / s, for 420 s to the temperature of the tumor tissue 46 ° C, when heating healthy tissues no higher than 40 ° C.