RU2735254C2 - Снижение табак-специфичных нитрозаминов в растениях - Google Patents

Снижение табак-специфичных нитрозаминов в растениях Download PDF

Info

Publication number
RU2735254C2
RU2735254C2 RU2015126909A RU2015126909A RU2735254C2 RU 2735254 C2 RU2735254 C2 RU 2735254C2 RU 2015126909 A RU2015126909 A RU 2015126909A RU 2015126909 A RU2015126909 A RU 2015126909A RU 2735254 C2 RU2735254 C2 RU 2735254C2
Authority
RU
Russia
Prior art keywords
plant
seq
mutant
tobacco plant
tobacco
Prior art date
Application number
RU2015126909A
Other languages
English (en)
Russian (ru)
Other versions
RU2015126909A (ru
Inventor
Люсьен БОВЕ
Приска КАМПАНОНИ
Original Assignee
Филип Моррис Продактс С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Филип Моррис Продактс С.А. filed Critical Филип Моррис Продактс С.А.
Publication of RU2015126909A publication Critical patent/RU2015126909A/ru
Application granted granted Critical
Publication of RU2735254C2 publication Critical patent/RU2735254C2/ru

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241—Extraction of specific substances
    • A24B15/245—Nitrosamines
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Manufacture Of Tobacco Products (AREA)
RU2015126909A 2012-12-21 2013-12-19 Снижение табак-специфичных нитрозаминов в растениях RU2735254C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12198966 2012-12-21
EP12198966.9 2012-12-21
PCT/EP2013/077532 WO2014096283A2 (en) 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants

Publications (2)

Publication Number Publication Date
RU2015126909A RU2015126909A (ru) 2017-01-27
RU2735254C2 true RU2735254C2 (ru) 2020-10-29

Family

ID=47559184

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2015126909A RU2735254C2 (ru) 2012-12-21 2013-12-19 Снижение табак-специфичных нитрозаминов в растениях

Country Status (11)

Country Link
US (1) US10563215B2 (enExample)
EP (1) EP2935588B1 (enExample)
JP (1) JP6693747B2 (enExample)
KR (1) KR102253223B1 (enExample)
CN (1) CN105247053B (enExample)
AP (1) AP2015008563A0 (enExample)
CA (1) CA2894955C (enExample)
MX (2) MX2015008084A (enExample)
PH (1) PH12015501204B1 (enExample)
RU (1) RU2735254C2 (enExample)
WO (1) WO2014096283A2 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9980509B2 (en) 2013-04-05 2018-05-29 R.J. Reynolds Tobacco Company Modification of bacterial profile of tobacco
EP3160988A2 (en) * 2014-06-25 2017-05-03 Philip Morris Products S.A. Modulation of biomass in plants by ectopic expression of a chloride channel
GB201501941D0 (en) * 2015-02-05 2015-03-25 British American Tobacco Co Method
HK1250883A1 (zh) * 2015-05-05 2019-01-18 North Carolina State University 减少烟草中的烟草特异性亚硝胺nnk的方法和组合物
WO2017156535A1 (en) * 2016-03-11 2017-09-14 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having reduced or eliminated suckers
JP2020529206A (ja) 2017-07-31 2020-10-08 アール・ジエイ・レイノルズ・タバコ・カンパニー 植物におけるウイルスベースの遺伝子編集のための方法及び組成物
WO2019185699A1 (en) 2018-03-28 2019-10-03 Philip Morris Products S.A. Modulating reducing sugar content in a plant
KR102995073B1 (ko) 2018-03-28 2026-07-24 필립모리스 프로덕츠 에스.에이. 식물 내 아미노산 함량 조절
GB201812603D0 (en) 2018-08-02 2018-09-19 British American Tobacco Investments Ltd Method
EP3902390A1 (en) * 2018-12-30 2021-11-03 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
WO2020247821A2 (en) * 2019-06-05 2020-12-10 R.J. Reynolds Tobacco Company bZIP TRANSCRIPTION FACTORS REGULATE CONVERSION OF NICOTINE TO NORNICOTINE AND REDUCE LEVELS OF TOBACCO SPECIFIC (TSNA) PRECURSORS
CN110499388B (zh) * 2019-09-29 2023-03-24 云南省烟草农业科学研究院 鉴别烟草抗斑萎病位点rtsw等位基因类型的共显性标记引物组、鉴别方法及其应用
WO2021063863A1 (en) 2019-10-01 2021-04-08 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (sultr3)
KR20220070305A (ko) 2019-10-01 2022-05-30 필립모리스 프로덕츠 에스.에이. 식물 내 환원당 (inv) 함량 조절
CN111690661A (zh) * 2020-06-01 2020-09-22 云南省烟草农业科学研究院 一种烟草NtbHLH13基因突变体及分子鉴定方法和应用
US20240368615A1 (en) 2021-09-10 2024-11-07 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
US20250084427A1 (en) 2021-12-21 2025-03-13 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN118475234A (zh) * 2021-12-27 2024-08-09 日本烟草产业株式会社 烟草植物
KR20250078994A (ko) 2022-10-13 2025-06-04 필립모리스 프로덕츠 에스.에이. Ntp2 조절에 의한 잎 바이오매스 및 질소 사용 효율 증가
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
WO2024160860A1 (en) 2023-02-02 2024-08-08 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
CN117025626B (zh) * 2023-06-29 2025-02-14 中国烟草总公司郑州烟草研究院 烟草硝酸盐转运蛋白NtNPF7.4及其编码基因、基因编辑载体和应用
CN117025627B (zh) * 2023-06-29 2024-11-01 中国烟草总公司郑州烟草研究院 烟草氯离子通道蛋白NtCLC13及其编码基因和应用
CN120944912B (zh) * 2025-09-17 2026-05-08 河南省农业科学院烟草研究所 NtGCN1基因及其在调控烟叶氯离子含量中的应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056923A1 (en) * 1997-06-12 1998-12-17 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US20070034220A1 (en) * 2003-08-19 2007-02-15 22Nd Century Limited, Llc Reduced-exposure tobacco products
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2011048009A1 (en) * 2009-10-22 2011-04-28 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5810020A (en) 1993-09-07 1998-09-22 Osmotek, Inc. Process for removing nitrogen-containing anions and tobacco-specific nitrosamines from tobacco products
US6202649B1 (en) 1996-12-02 2001-03-20 Regent Court Technologies Method of treating tobacco to reduce nitrosamine content, and products produced thereby
EE9900590A (et) 1997-06-20 2000-08-15 Regent Court Technologies Nitrosamiinide sisaldust vähendav tubaka töötlemise meetod ja seejuures saadavad tooted
US7700834B2 (en) * 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
RU2191529C1 (ru) 2001-12-21 2002-10-27 Романенко Александр Павлович Устройство для курения
DK1623634T3 (da) * 2003-05-06 2013-07-15 Japan Tobacco Inc Fremgangsmåde til fremstilling af regenereret tobaksmateriale
JP4160868B2 (ja) * 2003-07-10 2008-10-08 日本たばこ産業株式会社 葉タバコ中のニトロソアミン含量の低減方法
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
AU2007329776B2 (en) 2006-10-13 2013-05-16 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
ES2716864T3 (es) 2007-06-06 2019-06-17 Monsanto Technology Llc Genes y usos para la mejora de plantas
CN101939431B (zh) 2007-11-12 2015-01-28 北卡罗来纳州立大学 通过修饰特定细胞色素p450基因改变烟草生物碱含量
AP3226A (en) 2007-12-13 2015-04-30 Philip Morris Products Sa Transgenic plants modifier for reducede cadmium transport, derivative products, and related methods
WO2009091518A2 (en) * 2008-01-15 2009-07-23 Monsanto Technology, Llc Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
MX337123B (es) 2010-01-15 2016-02-12 Univ North Carolina State Composiciones y metodos para minimizar la sintesis de nornicotina en el tabaco.
ES2698154T3 (es) 2010-09-03 2019-01-31 Philip Morris Products Sa Reducción de metales pesados en plantas

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056923A1 (en) * 1997-06-12 1998-12-17 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US20070034220A1 (en) * 2003-08-19 2007-02-15 22Nd Century Limited, Llc Reduced-exposure tobacco products
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2011048009A1 (en) * 2009-10-22 2011-04-28 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
База данных: EMBL-EBI: Q9XF71 от 01.11.1999 *
База данных: EMBL-EBI: Q9XF71 от 01.11.1999. *

Also Published As

Publication number Publication date
PH12015501204A1 (en) 2015-08-17
KR102253223B1 (ko) 2021-05-21
AP2015008563A0 (en) 2015-06-30
CN105247053A (zh) 2016-01-13
JP6693747B2 (ja) 2020-05-13
WO2014096283A2 (en) 2014-06-26
EP2935588B1 (en) 2026-03-25
MX2019001598A (es) 2019-07-01
US20150315603A1 (en) 2015-11-05
RU2015126909A (ru) 2017-01-27
MX2015008084A (es) 2016-03-31
PH12015501204B1 (en) 2022-07-15
CA2894955A1 (en) 2014-06-26
EP2935588C0 (en) 2026-03-25
US10563215B2 (en) 2020-02-18
JP2016508032A (ja) 2016-03-17
CA2894955C (en) 2023-10-31
KR20150096451A (ko) 2015-08-24
CN105247053B (zh) 2020-07-31
EP2935588A2 (en) 2015-10-28
WO2014096283A3 (en) 2014-08-14

Similar Documents

Publication Publication Date Title
KR102253223B1 (ko) 식물 내 담배 특이 니트로사민 감소
JP7368579B2 (ja) 葉の中の１種または複数種のアルカロイドの量を変化させたタバコ植物ならびにそのような植物の使用方法
KR102207550B1 (ko) 감소된 아스파라긴 함량을 갖는 식물
RU2756102C2 (ru) Гены протеаз табака
KR102606956B1 (ko) 개화까지의 시간이 단축된 식물
KR102136088B1 (ko) 식물계에서의 중금속 감소
KR20170020416A (ko) 식물 내의 질산염 함량 조절
KR102012993B1 (ko) 니코티아나 타바쿰으로부터의 이소프로필말레이트 합성효소 및 그의 방법 및 용도
RU2733837C2 (ru) Снижение превращения никотина в норникотин в растениях
JP6302407B2 (ja) 植物におけるβ−ダマセノンの調節
KR20200136921A (ko) 식물 내 아미노산 함량 조절
KR20220070305A (ko) 식물 내 환원당 (inv) 함량 조절
KR102826087B1 (ko) 식물 내 환원당 함량 조절
CN114514321A (zh) 调节植物中的糖和氨基酸含量（sultr3）
JP2025134829A (ja) 吸枝が低減または消失したタバコ植物および製品を産生するための組成物および方法
RU2842948C1 (ru) Модулирование содержания редуцирующих сахаров в растении (inv)
RU2826107C1 (ru) Модулирование содержания сахаров и аминокислот в растении (sultr3)
RU2801948C2 (ru) Модулирование содержания редуцирующих сахаров в растении
RU2792235C2 (ru) Растения с сокращенным периодом времени до наступления цветения
RU2799785C2 (ru) Модулирование содержания аминокислот в растении
KR20220038605A (ko) 식물에서 알칼로이드 함량을 조정하는 방법
EP2586792A1 (en) Modulating beta-damascenone in plants
HK1196828A (en) Modulating beta-damascenone in plants
HK1196828B (en) Modulating beta-damascenone in plants