RU2735254C2 - Снижение табак-специфичных нитрозаминов в растениях - Google Patents

Снижение табак-специфичных нитрозаминов в растениях Download PDF

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Publication number
RU2735254C2
RU2735254C2 RU2015126909A RU2015126909A RU2735254C2 RU 2735254 C2 RU2735254 C2 RU 2735254C2 RU 2015126909 A RU2015126909 A RU 2015126909A RU 2015126909 A RU2015126909 A RU 2015126909A RU 2735254 C2 RU2735254 C2 RU 2735254C2
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plant
seq
mutant
tobacco plant
tobacco
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RU2015126909A
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Russian (ru)
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RU2015126909A (ru
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Люсьен БОВЕ
Приска КАМПАНОНИ
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Филип Моррис Продактс С.А.
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/24Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241Extraction of specific substances
    • A24B15/245Nitrosamines
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00Preparing tobacco in the factory
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Manufacture Of Tobacco Products (AREA)
RU2015126909A 2012-12-21 2013-12-19 Снижение табак-специфичных нитрозаминов в растениях RU2735254C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12198966 2012-12-21
EP12198966.9 2012-12-21
PCT/EP2013/077532 WO2014096283A2 (en) 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants

Publications (2)

Publication Number Publication Date
RU2015126909A RU2015126909A (ru) 2017-01-27
RU2735254C2 true RU2735254C2 (ru) 2020-10-29

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RU2015126909A RU2735254C2 (ru) 2012-12-21 2013-12-19 Снижение табак-специфичных нитрозаминов в растениях

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US (1) US10563215B2 (enExample)
EP (1) EP2935588A2 (enExample)
JP (1) JP6693747B2 (enExample)
KR (1) KR102253223B1 (enExample)
CN (1) CN105247053B (enExample)
AP (1) AP2015008563A0 (enExample)
CA (1) CA2894955C (enExample)
MX (2) MX2015008084A (enExample)
PH (1) PH12015501204B1 (enExample)
RU (1) RU2735254C2 (enExample)
WO (1) WO2014096283A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9980509B2 (en) 2013-04-05 2018-05-29 R.J. Reynolds Tobacco Company Modification of bacterial profile of tobacco
AR101243A1 (es) * 2014-06-25 2016-12-07 Philip Morris Products Sa Modulación del contenido de nitrato en plantas
GB201501941D0 (en) 2015-02-05 2015-03-25 British American Tobacco Co Method
US20180291388A1 (en) * 2015-05-05 2018-10-11 North Carolina State University Methods and compositions for reducing the tobacco specific nitrosamine nnk in tobacco
CN117126815A (zh) * 2016-03-11 2023-11-28 奥驰亚客户服务有限公司 用于生产烟杈减少或消除的烟草植物和制品的组合物和方法
EP3661354A1 (en) 2017-07-31 2020-06-10 R. J. Reynolds Tobacco Company Methods and compositions for viral-based gene editing in plants
JP7525400B2 (ja) 2018-03-28 2024-07-30 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体における還元糖含有量の調節
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
MX2021007628A (es) 2018-12-30 2021-08-11 Philip Morris Products Sa Modulacion de niveles de nitrato en plantas a traves de la mutacion de la nitrato reductasa.
WO2020247821A2 (en) * 2019-06-05 2020-12-10 R.J. Reynolds Tobacco Company bZIP TRANSCRIPTION FACTORS REGULATE CONVERSION OF NICOTINE TO NORNICOTINE AND REDUCE LEVELS OF TOBACCO SPECIFIC (TSNA) PRECURSORS
CN110499388B (zh) * 2019-09-29 2023-03-24 云南省烟草农业科学研究院 鉴别烟草抗斑萎病位点rtsw等位基因类型的共显性标记引物组、鉴别方法及其应用
JP7659547B2 (ja) 2019-10-01 2025-04-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における還元糖含有量の調節(inv)
WO2021063863A1 (en) 2019-10-01 2021-04-08 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (sultr3)
CN111690661A (zh) * 2020-06-01 2020-09-22 云南省烟草农业科学研究院 一种烟草NtbHLH13基因突变体及分子鉴定方法和应用
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
US20250084427A1 (en) 2021-12-21 2025-03-13 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
EP4458140A4 (en) * 2021-12-27 2025-10-15 Japan Tobacco Inc TOBACCO PLANT
KR20250078994A (ko) 2022-10-13 2025-06-04 필립모리스 프로덕츠 에스.에이. Ntp2 조절에 의한 잎 바이오매스 및 질소 사용 효율 증가
WO2024160864A1 (en) 2023-02-02 2024-08-08 Philip Morris Products S.A. Modulation of sugar transporters
WO2024160860A1 (en) 2023-02-02 2024-08-08 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
CN117025626B (zh) * 2023-06-29 2025-02-14 中国烟草总公司郑州烟草研究院 烟草硝酸盐转运蛋白NtNPF7.4及其编码基因、基因编辑载体和应用
CN121335615A (zh) 2023-06-29 2026-01-13 菲利普莫里斯生产公司 编码谷氨酸脱氢酶的基因的调节
CN117025627B (zh) * 2023-06-29 2024-11-01 中国烟草总公司郑州烟草研究院 烟草氯离子通道蛋白NtCLC13及其编码基因和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056923A1 (en) * 1997-06-12 1998-12-17 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US20070034220A1 (en) * 2003-08-19 2007-02-15 22Nd Century Limited, Llc Reduced-exposure tobacco products
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2011048009A1 (en) * 2009-10-22 2011-04-28 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5810020A (en) 1993-09-07 1998-09-22 Osmotek, Inc. Process for removing nitrogen-containing anions and tobacco-specific nitrosamines from tobacco products
US6202649B1 (en) 1996-12-02 2001-03-20 Regent Court Technologies Method of treating tobacco to reduce nitrosamine content, and products produced thereby
EP0991329A4 (en) 1997-06-20 2006-06-14 Regent Court Technologies METHOD OF TABAK TREATMENT FOR REDUCING NITROSAMINE CONTENT AND PRODUCTS MANUFACTURED THEREOF
US7700834B2 (en) * 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
RU2191529C1 (ru) 2001-12-21 2002-10-27 Романенко Александр Павлович Устройство для курения
WO2004098323A1 (ja) * 2003-05-06 2004-11-18 Japan Tobacco Inc. 再生タバコ材の製造方法
JP4160868B2 (ja) 2003-07-10 2008-10-08 日本たばこ産業株式会社 葉タバコ中のニトロソアミン含量の低減方法
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
BRPI0717355B1 (pt) 2006-10-13 2018-01-16 North Carolina State University Método para obtenção de uma planta transgênica de nicotina, método para obtenção de uma semente; construto de ácido nucléico recombinante; método de redução da conversão de nicotina em nornicotina em uma planta de nicotina
EP2840142B1 (en) 2007-06-06 2018-12-26 Monsanto Technology LLC Genes and uses for plant enhancement
CN101939431B (zh) 2007-11-12 2015-01-28 北卡罗来纳州立大学 通过修饰特定细胞色素p450基因改变烟草生物碱含量
WO2009074325A1 (en) 2007-12-13 2009-06-18 Philip Morris Products S.A. Transgenic plants modified for reduced cadmium transport, derivative products, and related methods
WO2009091518A2 (en) * 2008-01-15 2009-07-23 Monsanto Technology, Llc Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
US9247706B2 (en) 2010-01-15 2016-02-02 North Carolina State University Compositions and methods for minimizing nornicotine synthesis in tobacco
CN103228671B (zh) 2010-09-03 2017-08-15 菲利普莫里斯产品有限公司 植物中的重金属减少

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056923A1 (en) * 1997-06-12 1998-12-17 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US20070034220A1 (en) * 2003-08-19 2007-02-15 22Nd Century Limited, Llc Reduced-exposure tobacco products
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2011048009A1 (en) * 2009-10-22 2011-04-28 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
База данных: EMBL-EBI: Q9XF71 от 01.11.1999 *
База данных: EMBL-EBI: Q9XF71 от 01.11.1999. *

Also Published As

Publication number Publication date
PH12015501204B1 (en) 2022-07-15
MX2015008084A (es) 2016-03-31
JP2016508032A (ja) 2016-03-17
RU2015126909A (ru) 2017-01-27
JP6693747B2 (ja) 2020-05-13
EP2935588A2 (en) 2015-10-28
CN105247053B (zh) 2020-07-31
KR102253223B1 (ko) 2021-05-21
WO2014096283A3 (en) 2014-08-14
MX2019001598A (es) 2019-07-01
US20150315603A1 (en) 2015-11-05
CN105247053A (zh) 2016-01-13
CA2894955A1 (en) 2014-06-26
CA2894955C (en) 2023-10-31
WO2014096283A2 (en) 2014-06-26
PH12015501204A1 (en) 2015-08-17
US10563215B2 (en) 2020-02-18
AP2015008563A0 (en) 2015-06-30
KR20150096451A (ko) 2015-08-24

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