JP6693747B2 - 植物体中のたばこ特異的ニトロソアミンの低減 - Google Patents

Info

Publication number

JP6693747B2

JP6693747B2 JP2015548608A JP2015548608A JP6693747B2 JP 6693747 B2 JP6693747 B2 JP 6693747B2 JP 2015548608 A JP2015548608 A JP 2015548608A JP 2015548608 A JP2015548608 A JP 2015548608A JP 6693747 B2 JP6693747 B2 JP 6693747B2

Authority

JP

Japan

Prior art keywords

plant

seq

plants

tobacco

sequence

Prior art date

2012-12-21

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Active

Links

• Espacenet
• Global Dossier
• Discuss

• 241000196324 Embryophyta Species 0.000 title claims description 742
• 235000002637 Nicotiana tabacum Nutrition 0.000 title claims description 187
• 241000208125 Nicotiana Species 0.000 title claims description 8
• 150000004005 nitrosamines Chemical class 0.000 title claims description 8
• 230000009467 reduction Effects 0.000 title description 33
• 102000040430 polynucleotide Human genes 0.000 claims description 225
• 108091033319 polynucleotide Proteins 0.000 claims description 225
• 239000002157 polynucleotide Substances 0.000 claims description 224
• 230000035772 mutation Effects 0.000 claims description 203
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 176
• 238000000034 method Methods 0.000 claims description 167
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 167
• 229920001184 polypeptide Polymers 0.000 claims description 165
• 244000061176 Nicotiana tabacum Species 0.000 claims description 142
• 229910002651 NO3 Inorganic materials 0.000 claims description 132
• NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 132
• 230000014509 gene expression Effects 0.000 claims description 108
• 239000000463 material Substances 0.000 claims description 86
• 230000000694 effects Effects 0.000 claims description 70
• 230000002829 reductive effect Effects 0.000 claims description 58
• SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 claims description 57
• SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 claims description 57
• 229960002715 nicotine Drugs 0.000 claims description 57
• 230000001105 regulatory effect Effects 0.000 claims description 42
• 108010062745 Chloride Channels Proteins 0.000 claims description 25
• 102000011045 Chloride Channels Human genes 0.000 claims description 25
• 238000001035 drying Methods 0.000 claims description 25
• 108091064523 Clc family Proteins 0.000 claims description 19
• 235000019505 tobacco product Nutrition 0.000 claims description 19
• 238000004519 manufacturing process Methods 0.000 claims description 18
• 108700028369 Alleles Proteins 0.000 claims description 12
• 108091026890 Coding region Proteins 0.000 claims description 12
• 239000002028 Biomass Substances 0.000 claims description 11
• 238000003306 harvesting Methods 0.000 claims description 8
• 238000012545 processing Methods 0.000 claims description 5
• ALJXLZZGCGIWME-UHFFFAOYSA-N 5-(1-methylpyrrolidin-2-yl)-2-nitrosopyridine Chemical compound N(=O)C1=NC=C(C=C1)C1N(C)CCC1 ALJXLZZGCGIWME-UHFFFAOYSA-N 0.000 claims 1
• 241001044671 Piper tuberculatum Species 0.000 claims 1
• WRBCUESHYNADDF-UHFFFAOYSA-N n-methyl-n-(1-oxo-1-pyridin-3-ylbutan-2-yl)nitrous amide Chemical compound O=NN(C)C(CC)C(=O)C1=CC=CN=C1 WRBCUESHYNADDF-UHFFFAOYSA-N 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 description 176
• 239000002773 nucleotide Substances 0.000 description 106
• 125000003729 nucleotide group Chemical group 0.000 description 106
• 210000004027 cell Anatomy 0.000 description 97
• 229920002477 rna polymer Polymers 0.000 description 74
• 230000009261 transgenic effect Effects 0.000 description 72
• FLAQQSHRLBFIEZ-UHFFFAOYSA-N N-Methyl-N-nitroso-4-oxo-4-(3-pyridyl)butyl amine Chemical compound O=NN(C)CCCC(=O)C1=CC=CN=C1 FLAQQSHRLBFIEZ-UHFFFAOYSA-N 0.000 description 67
• 201000003478 cholangiolocellular carcinoma Diseases 0.000 description 63
• 241001144493 Nicotiana obtusifolia Species 0.000 description 55
• 102000053602 DNA Human genes 0.000 description 53
• 108020004414 DNA Proteins 0.000 description 51
• 102000004169 proteins and genes Human genes 0.000 description 50
• 150000007523 nucleic acids Chemical class 0.000 description 49
• 235000018102 proteins Nutrition 0.000 description 44
• 239000012634 fragment Substances 0.000 description 43
• 238000006467 substitution reaction Methods 0.000 description 42
• 102000039446 nucleic acids Human genes 0.000 description 41
• 108020004707 nucleic acids Proteins 0.000 description 41
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 38
• 230000002452 interceptive effect Effects 0.000 description 37
• 239000000523 sample Substances 0.000 description 36
• 230000000692 anti-sense effect Effects 0.000 description 33
• 210000001082 somatic cell Anatomy 0.000 description 33
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 32
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 32
• 108020004999 messenger RNA Proteins 0.000 description 32
• 239000004055 small Interfering RNA Substances 0.000 description 31
• OLHAGLWIVDYPQZ-UHFFFAOYSA-N 5-(1-methylpyrrolidin-2-yl)-1-nitroso-2H-pyridine Chemical compound CN1CCCC1C1=CN(N=O)CC=C1 OLHAGLWIVDYPQZ-UHFFFAOYSA-N 0.000 description 29
• 108020004459 Small interfering RNA Proteins 0.000 description 29
• 235000001014 amino acid Nutrition 0.000 description 29
• 239000013615 primer Substances 0.000 description 29
• 210000001519 tissue Anatomy 0.000 description 29
• 230000001965 increasing effect Effects 0.000 description 27
• 239000013598 vector Substances 0.000 description 27
• 239000013604 expression vector Substances 0.000 description 26
• 230000002068 genetic effect Effects 0.000 description 25
• 108091028043 Nucleic acid sequence Proteins 0.000 description 24
• 238000002703 mutagenesis Methods 0.000 description 23
• 231100000350 mutagenesis Toxicity 0.000 description 23
• 230000004048 modification Effects 0.000 description 21
• 238000012986 modification Methods 0.000 description 21
• 239000000047 product Substances 0.000 description 21
• 238000003780 insertion Methods 0.000 description 20
• 230000037431 insertion Effects 0.000 description 20
• 239000000203 mixture Substances 0.000 description 20
• 229940024606 amino acid Drugs 0.000 description 19
• 150000001413 amino acids Chemical class 0.000 description 19
• 230000000295 complement effect Effects 0.000 description 19
• 239000000443 aerosol Substances 0.000 description 18
• 230000000875 corresponding effect Effects 0.000 description 18
• 238000009396 hybridization Methods 0.000 description 18
• 230000001488 breeding effect Effects 0.000 description 17
• 238000012217 deletion Methods 0.000 description 17
• 230000037430 deletion Effects 0.000 description 17
• 238000004458 analytical method Methods 0.000 description 16
• 238000009395 breeding Methods 0.000 description 16
• 230000009368 gene silencing by RNA Effects 0.000 description 15
• 230000008569 process Effects 0.000 description 15
• 239000002987 primer (paints) Substances 0.000 description 14
• 101710185494 Zinc finger protein Proteins 0.000 description 13
• 102100023597 Zinc finger protein 816 Human genes 0.000 description 13
• 125000003275 alpha amino acid group Chemical group 0.000 description 13
• 230000003321 amplification Effects 0.000 description 13
• 230000030279 gene silencing Effects 0.000 description 13
• 238000003199 nucleic acid amplification method Methods 0.000 description 13
• 108090000994 Catalytic RNA Proteins 0.000 description 12
• 102000053642 Catalytic RNA Human genes 0.000 description 12
• 230000027455 binding Effects 0.000 description 12
• 230000003247 decreasing effect Effects 0.000 description 12
• 108091092562 ribozyme Proteins 0.000 description 12
• 230000009466 transformation Effects 0.000 description 12
• ZJOFAFWTOKDIFH-UHFFFAOYSA-N 3-(1-nitroso-3,6-dihydro-2h-pyridin-2-yl)pyridine Chemical compound O=NN1CC=CCC1C1=CC=CN=C1 ZJOFAFWTOKDIFH-UHFFFAOYSA-N 0.000 description 11
• 102000004190 Enzymes Human genes 0.000 description 11
• 108090000790 Enzymes Proteins 0.000 description 11
• 108700019146 Transgenes Proteins 0.000 description 11
• 230000007423 decrease Effects 0.000 description 11
• 238000002474 experimental method Methods 0.000 description 11
• 238000003752 polymerase chain reaction Methods 0.000 description 11
• 238000012216 screening Methods 0.000 description 11
• 239000000126 substance Substances 0.000 description 11
• 238000011144 upstream manufacturing Methods 0.000 description 11
• 108091034117 Oligonucleotide Proteins 0.000 description 10
• 230000015572 biosynthetic process Effects 0.000 description 10
• 238000003776 cleavage reaction Methods 0.000 description 10
• 238000001514 detection method Methods 0.000 description 10
• 125000006850 spacer group Chemical group 0.000 description 10
• 241000701489 Cauliflower mosaic virus Species 0.000 description 9
• 241000208136 Nicotiana sylvestris Species 0.000 description 9
• 108091030071 RNAI Proteins 0.000 description 9
• 108010017070 Zinc Finger Nucleases Proteins 0.000 description 9
• 238000013459 approach Methods 0.000 description 9
• 230000001404 mediated effect Effects 0.000 description 9
• 230000007017 scission Effects 0.000 description 9
• 241000894007 species Species 0.000 description 9
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
• 108020004635 Complementary DNA Proteins 0.000 description 8
• 241000208138 Nicotiana tomentosiformis Species 0.000 description 8
• 230000006870 function Effects 0.000 description 8
• -1 methylnitrosoamino Chemical group 0.000 description 8
• 238000003976 plant breeding Methods 0.000 description 8
• 238000000746 purification Methods 0.000 description 8
• 238000012546 transfer Methods 0.000 description 8
• PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 7
• IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 7
• 101710163270 Nuclease Proteins 0.000 description 7
• 108091081021 Sense strand Proteins 0.000 description 7
• 240000008042 Zea mays Species 0.000 description 7
• 230000000711 cancerogenic effect Effects 0.000 description 7
• 231100000315 carcinogenic Toxicity 0.000 description 7
• 239000003153 chemical reaction reagent Substances 0.000 description 7
• 230000004927 fusion Effects 0.000 description 7
• 238000010362 genome editing Methods 0.000 description 7
• 238000002844 melting Methods 0.000 description 7
• 230000008018 melting Effects 0.000 description 7
• 239000013612 plasmid Substances 0.000 description 7
• 238000013518 transcription Methods 0.000 description 7
• 230000035897 transcription Effects 0.000 description 7
• 230000002103 transcriptional effect Effects 0.000 description 7
• 108091033409 CRISPR Proteins 0.000 description 6
• 206010021929 Infertility male Diseases 0.000 description 6
• 208000007466 Male Infertility Diseases 0.000 description 6
• 241001465754 Metazoa Species 0.000 description 6
• 108020004511 Recombinant DNA Proteins 0.000 description 6
• 108091027967 Small hairpin RNA Proteins 0.000 description 6
• 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
• 229920002494 Zein Polymers 0.000 description 6
• 238000007605 air drying Methods 0.000 description 6
• 230000001580 bacterial effect Effects 0.000 description 6
• 230000033228 biological regulation Effects 0.000 description 6
• 238000010804 cDNA synthesis Methods 0.000 description 6
• 238000006243 chemical reaction Methods 0.000 description 6
• 239000002299 complementary DNA Substances 0.000 description 6
• 239000000306 component Substances 0.000 description 6
• 230000005782 double-strand break Effects 0.000 description 6
• 238000010353 genetic engineering Methods 0.000 description 6
• 239000003446 ligand Substances 0.000 description 6
• 239000003550 marker Substances 0.000 description 6
• 230000002441 reversible effect Effects 0.000 description 6
• 239000011734 sodium Substances 0.000 description 6
• 238000003786 synthesis reaction Methods 0.000 description 6
• 239000005019 zein Substances 0.000 description 6
• 229940093612 zein Drugs 0.000 description 6
• 241000589158 Agrobacterium Species 0.000 description 5
• 108091079001 CRISPR RNA Proteins 0.000 description 5
• 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 description 5
• 235000007688 Lycopersicon esculentum Nutrition 0.000 description 5
• 102000000574 RNA-Induced Silencing Complex Human genes 0.000 description 5
• 108010016790 RNA-Induced Silencing Complex Proteins 0.000 description 5
• 240000003768 Solanum lycopersicum Species 0.000 description 5
• 108091081024 Start codon Proteins 0.000 description 5
• 238000009825 accumulation Methods 0.000 description 5
• 230000004075 alteration Effects 0.000 description 5
• 230000000890 antigenic effect Effects 0.000 description 5
• 230000003115 biocidal effect Effects 0.000 description 5
• 230000015556 catabolic process Effects 0.000 description 5
• 210000000349 chromosome Anatomy 0.000 description 5
• 230000001276 controlling effect Effects 0.000 description 5
• 238000007796 conventional method Methods 0.000 description 5
• 238000006731 degradation reaction Methods 0.000 description 5
• 238000012239 gene modification Methods 0.000 description 5
• 230000005017 genetic modification Effects 0.000 description 5
• 235000013617 genetically modified food Nutrition 0.000 description 5
• 238000003205 genotyping method Methods 0.000 description 5
• 229910001385 heavy metal Inorganic materials 0.000 description 5
• 230000004060 metabolic process Effects 0.000 description 5
• 102000054765 polymorphisms of proteins Human genes 0.000 description 5
• 230000032361 posttranscriptional gene silencing Effects 0.000 description 5
• 230000000306 recurrent effect Effects 0.000 description 5
• 238000003757 reverse transcription PCR Methods 0.000 description 5
• 239000001509 sodium citrate Substances 0.000 description 5
• NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 5
• 238000010561 standard procedure Methods 0.000 description 5
• 238000012360 testing method Methods 0.000 description 5
• 239000010409 thin film Substances 0.000 description 5
• 230000000007 visual effect Effects 0.000 description 5
• 241000894006 Bacteria Species 0.000 description 4
• 230000004568 DNA-binding Effects 0.000 description 4
• 240000002395 Euphorbia pulcherrima Species 0.000 description 4
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
• 239000005562 Glyphosate Substances 0.000 description 4
• 244000020551 Helianthus annuus Species 0.000 description 4
• 235000003222 Helianthus annuus Nutrition 0.000 description 4
• 206010020649 Hyperkeratosis Diseases 0.000 description 4
• 108020005350 Initiator Codon Proteins 0.000 description 4
• 240000004658 Medicago sativa Species 0.000 description 4
• 108091092878 Microsatellite Proteins 0.000 description 4
• 241000219000 Populus Species 0.000 description 4
• 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 4
• 108091028113 Trans-activating crRNA Proteins 0.000 description 4
• 241000700605 Viruses Species 0.000 description 4
• 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
• 238000003556 assay Methods 0.000 description 4
• 230000001413 cellular effect Effects 0.000 description 4
• 239000000470 constituent Substances 0.000 description 4
• 230000001419 dependent effect Effects 0.000 description 4
• 238000013461 design Methods 0.000 description 4
• 238000011161 development Methods 0.000 description 4
• 230000018109 developmental process Effects 0.000 description 4
• 229940097068 glyphosate Drugs 0.000 description 4
• XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 4
• 238000007901 in situ hybridization Methods 0.000 description 4
• 238000000338 in vitro Methods 0.000 description 4
• 238000001727 in vivo Methods 0.000 description 4
• 235000009973 maize Nutrition 0.000 description 4
• 231100000219 mutagenic Toxicity 0.000 description 4
• 239000003471 mutagenic agent Substances 0.000 description 4
• 231100000707 mutagenic chemical Toxicity 0.000 description 4
• 230000003505 mutagenic effect Effects 0.000 description 4
• IIDMFFRDEFHWCJ-UHFFFAOYSA-N n-(4-hydroxy-1-pyridin-3-ylbutyl)-n-methylnitrous amide Chemical compound OCCCC(N(N=O)C)C1=CC=CN=C1 IIDMFFRDEFHWCJ-UHFFFAOYSA-N 0.000 description 4
• 229910052757 nitrogen Inorganic materials 0.000 description 4
• 210000001938 protoplast Anatomy 0.000 description 4
• 230000010076 replication Effects 0.000 description 4
• 230000010153 self-pollination Effects 0.000 description 4
• 238000012163 sequencing technique Methods 0.000 description 4
• 230000000391 smoking effect Effects 0.000 description 4
• 239000000243 solution Substances 0.000 description 4
• 230000001131 transforming effect Effects 0.000 description 4
• 238000013519 translation Methods 0.000 description 4
• 230000032258 transport Effects 0.000 description 4
• 229910052725 zinc Inorganic materials 0.000 description 4
• 239000011701 zinc Substances 0.000 description 4
• MYKUKUCHPMASKF-VIFPVBQESA-N (S)-nornicotine Chemical compound C1CCN[C@@H]1C1=CC=CN=C1 MYKUKUCHPMASKF-VIFPVBQESA-N 0.000 description 3
• OGRXKBUCZFFSTL-UHFFFAOYSA-N 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol Chemical compound O=NN(C)CCCC(O)C1=CC=CN=C1 OGRXKBUCZFFSTL-UHFFFAOYSA-N 0.000 description 3
• 241000208140 Acer Species 0.000 description 3
• 229920001817 Agar Polymers 0.000 description 3
• 244000099147 Ananas comosus Species 0.000 description 3
• 108020005544 Antisense RNA Proteins 0.000 description 3
• 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 3
• 240000003259 Brassica oleracea var. botrytis Species 0.000 description 3
• 238000010354 CRISPR gene editing Methods 0.000 description 3
• 108010078791 Carrier Proteins Proteins 0.000 description 3
• 108091006146 Channels Proteins 0.000 description 3
• 235000009854 Cucurbita moschata Nutrition 0.000 description 3
• 108010042407 Endonucleases Proteins 0.000 description 3
• 102000004533 Endonucleases Human genes 0.000 description 3
• 244000166124 Eucalyptus globulus Species 0.000 description 3
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
• 241000209082 Lolium Species 0.000 description 3
• MYKUKUCHPMASKF-UHFFFAOYSA-N Nornicotine Natural products C1CCNC1C1=CC=CN=C1 MYKUKUCHPMASKF-UHFFFAOYSA-N 0.000 description 3
• 238000003559 RNA-seq method Methods 0.000 description 3
• 238000010240 RT-PCR analysis Methods 0.000 description 3
• 241000124033 Salix Species 0.000 description 3
• 235000007238 Secale cereale Nutrition 0.000 description 3
• 244000082988 Secale cereale Species 0.000 description 3
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
• 241000209072 Sorghum Species 0.000 description 3
• 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
• 241000251131 Sphyrna Species 0.000 description 3
• 235000021307 Triticum Nutrition 0.000 description 3
• 244000098338 Triticum aestivum Species 0.000 description 3
• 239000002671 adjuvant Substances 0.000 description 3
• 239000008272 agar Substances 0.000 description 3
• 229930013930 alkaloid Natural products 0.000 description 3
• 238000000137 annealing Methods 0.000 description 3
• 239000000872 buffer Substances 0.000 description 3
• 229910052793 cadmium Inorganic materials 0.000 description 3
• BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 3
• 125000002091 cationic group Chemical group 0.000 description 3
• 230000032823 cell division Effects 0.000 description 3
• 229930002875 chlorophyll Natural products 0.000 description 3
• 235000019804 chlorophyll Nutrition 0.000 description 3
• ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 3
• 230000002759 chromosomal effect Effects 0.000 description 3
• 235000019504 cigarettes Nutrition 0.000 description 3
• 150000001875 compounds Chemical class 0.000 description 3
• 235000013399 edible fruits Nutrition 0.000 description 3
• 238000004520 electroporation Methods 0.000 description 3
• 230000002255 enzymatic effect Effects 0.000 description 3
• 239000004009 herbicide Substances 0.000 description 3
• 210000004408 hybridoma Anatomy 0.000 description 3
• 230000000415 inactivating effect Effects 0.000 description 3
• 230000010354 integration Effects 0.000 description 3
• 229930027917 kanamycin Natural products 0.000 description 3
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
• 229960000318 kanamycin Drugs 0.000 description 3
• 229930182823 kanamycin A Natural products 0.000 description 3
• 230000000670 limiting effect Effects 0.000 description 3
• 230000004807 localization Effects 0.000 description 3
• 125000001921 locked nucleotide group Chemical group 0.000 description 3
• 230000007246 mechanism Effects 0.000 description 3
• 238000010369 molecular cloning Methods 0.000 description 3
• 230000006780 non-homologous end joining Effects 0.000 description 3
• 239000005022 packaging material Substances 0.000 description 3
• 244000052769 pathogen Species 0.000 description 3
• PTMHPRAIXMAOOB-UHFFFAOYSA-N phosphoramidic acid Chemical compound NP(O)(O)=O PTMHPRAIXMAOOB-UHFFFAOYSA-N 0.000 description 3
• 229940061584 phosphoramidic acid Drugs 0.000 description 3
• 230000005855 radiation Effects 0.000 description 3
• 230000001850 reproductive effect Effects 0.000 description 3
• 238000011160 research Methods 0.000 description 3
• 150000003839 salts Chemical class 0.000 description 3
• 238000002741 site-directed mutagenesis Methods 0.000 description 3
• 229910052708 sodium Inorganic materials 0.000 description 3
• 230000035882 stress Effects 0.000 description 3
• 241001515965 unidentified phage Species 0.000 description 3
• 238000005406 washing Methods 0.000 description 3
• LWTDZKXXJRRKDG-KXBFYZLASA-N (-)-phaseollin Chemical compound C1OC2=CC(O)=CC=C2[C@H]2[C@@H]1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-KXBFYZLASA-N 0.000 description 2
• 125000003349 3-pyridyl group Chemical group N1=C([H])C([*])=C([H])C([H])=C1[H] 0.000 description 2
• NZSNJPDBPMIBSP-UHFFFAOYSA-N 4-[methyl(nitroso)amino]-4-pyridin-3-ylbutanoic acid Chemical compound OC(=O)CCC(N(N=O)C)C1=CC=CN=C1 NZSNJPDBPMIBSP-UHFFFAOYSA-N 0.000 description 2
• 240000004507 Abelmoschus esculentus Species 0.000 description 2
• 241000218642 Abies Species 0.000 description 2
• 108010000700 Acetolactate synthase Proteins 0.000 description 2
• 241000743339 Agrostis Species 0.000 description 2
• 244000291564 Allium cepa Species 0.000 description 2
• QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
• 235000007119 Ananas comosus Nutrition 0.000 description 2
• 241001327399 Andropogon gerardii Species 0.000 description 2
• 108020000948 Antisense Oligonucleotides Proteins 0.000 description 2
• 241000219194 Arabidopsis Species 0.000 description 2
• 241000219195 Arabidopsis thaliana Species 0.000 description 2
• 235000021533 Beta vulgaris Nutrition 0.000 description 2
• 241000335053 Beta vulgaris Species 0.000 description 2
• 241000219198 Brassica Species 0.000 description 2
• 235000017647 Brassica oleracea var italica Nutrition 0.000 description 2
• 240000004160 Capsicum annuum Species 0.000 description 2
• 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 2
• 235000003255 Carthamus tinctorius Nutrition 0.000 description 2
• 244000020518 Carthamus tinctorius Species 0.000 description 2
• 108010022172 Chitinases Proteins 0.000 description 2
• VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
• KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
• 244000241235 Citrullus lanatus Species 0.000 description 2
• 108020004705 Codon Proteins 0.000 description 2
• 241000723377 Coffea Species 0.000 description 2
• 241000219112 Cucumis Species 0.000 description 2
• 240000008067 Cucumis sativus Species 0.000 description 2
• 240000004244 Cucurbita moschata Species 0.000 description 2
• 240000001980 Cucurbita pepo Species 0.000 description 2
• 235000009852 Cucurbita pepo Nutrition 0.000 description 2
• HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 2
• 235000009355 Dianthus caryophyllus Nutrition 0.000 description 2
• 240000006497 Dianthus caryophyllus Species 0.000 description 2
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
• 238000002965 ELISA Methods 0.000 description 2
• 240000009088 Fragaria x ananassa Species 0.000 description 2
• 235000011363 Fragaria x ananassa Nutrition 0.000 description 2
• 241000233866 Fungi Species 0.000 description 2
• 206010064571 Gene mutation Diseases 0.000 description 2
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
• 235000010469 Glycine max Nutrition 0.000 description 2
• 244000068988 Glycine max Species 0.000 description 2
• 244000299507 Gossypium hirsutum Species 0.000 description 2
• 241000238631 Hexapoda Species 0.000 description 2
• 235000007340 Hordeum vulgare Nutrition 0.000 description 2
• 240000005979 Hordeum vulgare Species 0.000 description 2
• 206010021033 Hypomenorrhoea Diseases 0.000 description 2
• 241000221089 Jatropha Species 0.000 description 2
• 241000219745 Lupinus Species 0.000 description 2
• 240000003183 Manihot esculenta Species 0.000 description 2
• 235000010624 Medicago sativa Nutrition 0.000 description 2
• 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
• 102100021299 Methyl-CpG-binding domain protein 2 Human genes 0.000 description 2
• 240000003433 Miscanthus floridulus Species 0.000 description 2
• VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 2
• 108091061960 Naked DNA Proteins 0.000 description 2
• 241001230286 Narenga Species 0.000 description 2
• 241000493375 Nicotiana quadrivalvis Species 0.000 description 2
• 241001144500 Nicotiana rosulata Species 0.000 description 2
• 108091092724 Noncoding DNA Proteins 0.000 description 2
• 238000000636 Northern blotting Methods 0.000 description 2
• 240000007594 Oryza sativa Species 0.000 description 2
• 235000007164 Oryza sativa Nutrition 0.000 description 2
• 229910019142 PO4 Inorganic materials 0.000 description 2
• 241001520808 Panicum virgatum Species 0.000 description 2
• 240000007377 Petunia x hybrida Species 0.000 description 2
• 235000011613 Pinus brutia Nutrition 0.000 description 2
• 240000007909 Prosopis juliflora Species 0.000 description 2
• JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
• 101150111829 RBCS2 gene Proteins 0.000 description 2
• PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
• 235000011449 Rosa Nutrition 0.000 description 2
• MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 description 2
• 241000209051 Saccharum Species 0.000 description 2
• 108020005543 Satellite RNA Proteins 0.000 description 2
• 101000611441 Solanum lycopersicum Pathogenesis-related leaf protein 6 Proteins 0.000 description 2
• 235000002597 Solanum melongena Nutrition 0.000 description 2
• 244000061458 Solanum melongena Species 0.000 description 2
• 235000002595 Solanum tuberosum Nutrition 0.000 description 2
• 244000061456 Solanum tuberosum Species 0.000 description 2
• 238000002105 Southern blotting Methods 0.000 description 2
• 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 2
• 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 2
• 244000269722 Thea sinensis Species 0.000 description 2
• 244000299461 Theobroma cacao Species 0.000 description 2
• 235000009470 Theobroma cacao Nutrition 0.000 description 2
• RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical compound OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 2
• 108010073062 Transcription Activator-Like Effectors Proteins 0.000 description 2
• 102000040945 Transcription factor Human genes 0.000 description 2
• 108091023040 Transcription factor Proteins 0.000 description 2
• 241000219095 Vitis Species 0.000 description 2
• 235000009392 Vitis Nutrition 0.000 description 2
• 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
• 239000002253 acid Substances 0.000 description 2
• 230000004913 activation Effects 0.000 description 2
• HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
• 239000000074 antisense oligonucleotide Substances 0.000 description 2
• 238000012230 antisense oligonucleotides Methods 0.000 description 2
• 210000004507 artificial chromosome Anatomy 0.000 description 2
• 239000012237 artificial material Substances 0.000 description 2
• 230000008901 benefit Effects 0.000 description 2
• 230000004071 biological effect Effects 0.000 description 2
• 150000001768 cations Chemical class 0.000 description 2
• 238000007385 chemical modification Methods 0.000 description 2
• 239000002962 chemical mutagen Substances 0.000 description 2
• 239000003795 chemical substances by application Substances 0.000 description 2
• 238000004587 chromatography analysis Methods 0.000 description 2
• 238000002485 combustion reaction Methods 0.000 description 2
• 239000003184 complementary RNA Substances 0.000 description 2
• 238000004590 computer program Methods 0.000 description 2
• 235000005822 corn Nutrition 0.000 description 2
• 238000012258 culturing Methods 0.000 description 2
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
• 229910003460 diamond Inorganic materials 0.000 description 2
• 239000010432 diamond Substances 0.000 description 2
• NAGJZTKCGNOGPW-UHFFFAOYSA-N dithiophosphoric acid Chemical compound OP(O)(S)=S NAGJZTKCGNOGPW-UHFFFAOYSA-N 0.000 description 2
• 238000005516 engineering process Methods 0.000 description 2
• 239000003623 enhancer Substances 0.000 description 2
• 238000011156 evaluation Methods 0.000 description 2
• 239000000284 extract Substances 0.000 description 2
• 230000004345 fruit ripening Effects 0.000 description 2
• ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 2
• 230000013595 glycosylation Effects 0.000 description 2
• 238000006206 glycosylation reaction Methods 0.000 description 2
• UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
• 230000002363 herbicidal effect Effects 0.000 description 2
• 230000002209 hydrophobic effect Effects 0.000 description 2
• 230000001976 improved effect Effects 0.000 description 2
• 238000011065 in-situ storage Methods 0.000 description 2
• 230000002779 inactivation Effects 0.000 description 2
• 238000010348 incorporation Methods 0.000 description 2
• 230000001939 inductive effect Effects 0.000 description 2
• 230000002401 inhibitory effect Effects 0.000 description 2
• 230000003993 interaction Effects 0.000 description 2
• 238000002955 isolation Methods 0.000 description 2
• 238000006317 isomerization reaction Methods 0.000 description 2
• 238000002372 labelling Methods 0.000 description 2
• 210000001161 mammalian embryo Anatomy 0.000 description 2
• 238000004949 mass spectrometry Methods 0.000 description 2
• 230000002503 metabolic effect Effects 0.000 description 2
• 239000003147 molecular marker Substances 0.000 description 2
• 230000000877 morphologic effect Effects 0.000 description 2
• UMFJAHHVKNCGLG-UHFFFAOYSA-N n-Nitrosodimethylamine Chemical compound CN(C)N=O UMFJAHHVKNCGLG-UHFFFAOYSA-N 0.000 description 2
• FRJHUNPWTKLYGL-UHFFFAOYSA-N n-methyl-n-(4-oxo-1-pyridin-3-ylbutyl)nitrous amide Chemical compound O=CCCC(N(N=O)C)C1=CC=CN=C1 FRJHUNPWTKLYGL-UHFFFAOYSA-N 0.000 description 2
• 150000002823 nitrates Chemical class 0.000 description 2
• 230000009935 nitrosation Effects 0.000 description 2
• 238000007034 nitrosation reaction Methods 0.000 description 2
• 210000000056 organ Anatomy 0.000 description 2
• 239000001301 oxygen Substances 0.000 description 2
• 229910052760 oxygen Inorganic materials 0.000 description 2
• 239000002245 particle Substances 0.000 description 2
• 230000035515 penetration Effects 0.000 description 2
• 238000010647 peptide synthesis reaction Methods 0.000 description 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
• 239000010452 phosphate Substances 0.000 description 2
• 150000004713 phosphodiesters Chemical group 0.000 description 2
• 230000001766 physiological effect Effects 0.000 description 2
• 210000002706 plastid Anatomy 0.000 description 2
• 230000010152 pollination Effects 0.000 description 2
• 229920000642 polymer Polymers 0.000 description 2
• 230000001737 promoting effect Effects 0.000 description 2
• 230000006798 recombination Effects 0.000 description 2
• 230000008263 repair mechanism Effects 0.000 description 2
• 150000003290 ribose derivatives Chemical group 0.000 description 2
• 102200006189 rs137854599 Human genes 0.000 description 2
• 229930000044 secondary metabolite Natural products 0.000 description 2
• 230000007226 seed germination Effects 0.000 description 2
• 230000001568 sexual effect Effects 0.000 description 2
• 239000000779 smoke Substances 0.000 description 2
• SRWFBFUYENBCGF-UHFFFAOYSA-M sodium;chloride;hydrochloride Chemical compound [Na+].Cl.[Cl-] SRWFBFUYENBCGF-UHFFFAOYSA-M 0.000 description 2
• 238000010532 solid phase synthesis reaction Methods 0.000 description 2
• 230000000392 somatic effect Effects 0.000 description 2
• 239000000758 substrate Substances 0.000 description 2
• 235000000346 sugar Nutrition 0.000 description 2
• 210000002377 thylakoid Anatomy 0.000 description 2
• 230000005030 transcription termination Effects 0.000 description 2
• 229910052723 transition metal Inorganic materials 0.000 description 2
• 150000003624 transition metals Chemical class 0.000 description 2
• 230000005945 translocation Effects 0.000 description 2
• 210000003934 vacuole Anatomy 0.000 description 2
• 210000003462 vein Anatomy 0.000 description 2
• 239000013603 viral vector Substances 0.000 description 2
• 230000003612 virological effect Effects 0.000 description 2
• 238000001262 western blot Methods 0.000 description 2
• 241000228158 x Triticosecale Species 0.000 description 2
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
• ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 description 1
• 239000001707 (E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-ol Substances 0.000 description 1
• MTXSIJUGVMTTMU-JTQLQIEISA-N (S)-anabasine Chemical compound N1CCCC[C@H]1C1=CC=CN=C1 MTXSIJUGVMTTMU-JTQLQIEISA-N 0.000 description 1
• LRHPLDYGYMQRHN-UHFFFAOYSA-N 1-butanol Substances CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
• OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
• UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
• MWBWWFOAEOYUST-UHFFFAOYSA-N 2-aminopurine Chemical compound NC1=NC=C2N=CNC2=N1 MWBWWFOAEOYUST-UHFFFAOYSA-N 0.000 description 1
• LNCCBHFAHILMCT-UHFFFAOYSA-N 2-n,4-n,6-n-triethyl-1,3,5-triazine-2,4,6-triamine Chemical compound CCNC1=NC(NCC)=NC(NCC)=N1 LNCCBHFAHILMCT-UHFFFAOYSA-N 0.000 description 1
• HEGWNIMGIDYRAU-UHFFFAOYSA-N 3-hexyl-2,4-dioxabicyclo[1.1.0]butane Chemical compound O1C2OC21CCCCCC HEGWNIMGIDYRAU-UHFFFAOYSA-N 0.000 description 1
• 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
• AJBZENLMTKDAEK-UHFFFAOYSA-N 3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysene-4,9-diol Chemical compound CC12CCC(O)C(C)(C)C1CCC(C1(C)CC3O)(C)C2CCC1C1C3(C)CCC1C(=C)C AJBZENLMTKDAEK-UHFFFAOYSA-N 0.000 description 1
• ARSRBNBHOADGJU-UHFFFAOYSA-N 7,12-dimethyltetraphene Chemical compound C1=CC2=CC=CC=C2C2=C1C(C)=C(C=CC=C1)C1=C2C ARSRBNBHOADGJU-UHFFFAOYSA-N 0.000 description 1
• 241001075517 Abelmoschus Species 0.000 description 1
• 235000003934 Abelmoschus esculentus Nutrition 0.000 description 1
• 241000207965 Acanthaceae Species 0.000 description 1
• HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
• 241001136782 Alca Species 0.000 description 1
• 241000123646 Allioideae Species 0.000 description 1
• 241000234282 Allium Species 0.000 description 1
• 235000005255 Allium cepa Nutrition 0.000 description 1
• 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
• 241000556588 Alstroemeria Species 0.000 description 1
• 241000556591 Alstroemeriaceae Species 0.000 description 1
• 240000008025 Alternanthera ficoidea Species 0.000 description 1
• 241000219317 Amaranthaceae Species 0.000 description 1
• 244000300297 Amaranthus hybridus Species 0.000 description 1
• 241000234270 Amaryllidaceae Species 0.000 description 1
• 241000746375 Andrographis Species 0.000 description 1
• 241000744007 Andropogon Species 0.000 description 1
• 102000003669 Antiporters Human genes 0.000 description 1
• 108090000084 Antiporters Proteins 0.000 description 1
• 241000208327 Apocynaceae Species 0.000 description 1
• 108700019794 Arabidopsis Myb Proteins 0.000 description 1
• 241000203069 Archaea Species 0.000 description 1
• 241000233788 Arecaceae Species 0.000 description 1
• 239000004475 Arginine Substances 0.000 description 1
• 235000003826 Artemisia Nutrition 0.000 description 1
• 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
• 241001494510 Arundo Species 0.000 description 1
• 241001494508 Arundo donax Species 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 241000208838 Asteraceae Species 0.000 description 1
• 241001106067 Atropa Species 0.000 description 1
• 241000726301 Avocado sunblotch viroid Species 0.000 description 1
• 235000000832 Ayote Nutrition 0.000 description 1
• 241000193388 Bacillus thuringiensis Species 0.000 description 1
• 101100497219 Bacillus thuringiensis subsp. kurstaki cry1Ac gene Proteins 0.000 description 1
• 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
• 235000017491 Bambusa tulda Nutrition 0.000 description 1
• 241000133570 Berberidaceae Species 0.000 description 1
• 240000000724 Berberis vulgaris Species 0.000 description 1
• 241000934840 Bixa Species 0.000 description 1
• 235000006011 Bixa Nutrition 0.000 description 1
• 241000934828 Bixaceae Species 0.000 description 1
• 108010006654 Bleomycin Proteins 0.000 description 1
• 241000339490 Brachyachne Species 0.000 description 1
• 235000011331 Brassica Nutrition 0.000 description 1
• 235000011303 Brassica alboglabra Nutrition 0.000 description 1
• 235000003351 Brassica cretica Nutrition 0.000 description 1
• 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
• 240000002791 Brassica napus Species 0.000 description 1
• 235000011293 Brassica napus Nutrition 0.000 description 1
• 235000006008 Brassica napus var napus Nutrition 0.000 description 1
• 240000000385 Brassica napus var. napus Species 0.000 description 1
• 240000007124 Brassica oleracea Species 0.000 description 1
• 235000011302 Brassica oleracea Nutrition 0.000 description 1
• 235000004221 Brassica oleracea var gemmifera Nutrition 0.000 description 1
• 244000308368 Brassica oleracea var. gemmifera Species 0.000 description 1
• 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
• 235000003343 Brassica rupestris Nutrition 0.000 description 1
• 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
• 241000219193 Brassicaceae Species 0.000 description 1
• 241000234670 Bromeliaceae Species 0.000 description 1
• 101100394003 Butyrivibrio fibrisolvens end1 gene Proteins 0.000 description 1
• 238000010453 CRISPR/Cas method Methods 0.000 description 1
• 101100342815 Caenorhabditis elegans lec-1 gene Proteins 0.000 description 1
• 235000003880 Calendula Nutrition 0.000 description 1
• 240000001432 Calendula officinalis Species 0.000 description 1
• 241000209507 Camellia Species 0.000 description 1
• 241000759909 Camptotheca Species 0.000 description 1
• 241000218235 Cannabaceae Species 0.000 description 1
• 241000218236 Cannabis Species 0.000 description 1
• 240000008574 Capsicum frutescens Species 0.000 description 1
• 206010007269 Carcinogenicity Diseases 0.000 description 1
• 241000219321 Caryophyllaceae Species 0.000 description 1
• 241000488900 Cephalotaxaceae Species 0.000 description 1
• 241000488899 Cephalotaxus Species 0.000 description 1
• 241000282985 Cervus Species 0.000 description 1
• 102000012286 Chitinases Human genes 0.000 description 1
• 239000005496 Chlorsulfuron Substances 0.000 description 1
• 235000007516 Chrysanthemum Nutrition 0.000 description 1
• 240000005250 Chrysanthemum indicum Species 0.000 description 1
• 235000021513 Cinchona Nutrition 0.000 description 1
• 241000157855 Cinchona Species 0.000 description 1
• 108091028075 Circular RNA Proteins 0.000 description 1
• 241000219109 Citrullus Species 0.000 description 1
• 235000009831 Citrullus lanatus Nutrition 0.000 description 1
• 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
• 108091033380 Coding strand Proteins 0.000 description 1
• 241000131506 Colchicaceae Species 0.000 description 1
• 241000723375 Colchicum Species 0.000 description 1
• 235000021508 Coleus Nutrition 0.000 description 1
• 244000061182 Coleus blumei Species 0.000 description 1
• 108091004554 Copper Transport Proteins Proteins 0.000 description 1
• 108091006566 Copper transporters Proteins 0.000 description 1
• 102000037773 Copper transporters Human genes 0.000 description 1
• 241000186216 Corynebacterium Species 0.000 description 1
• 229920000742 Cotton Polymers 0.000 description 1
• 101710190853 Cruciferin Proteins 0.000 description 1
• 244000241257 Cucumis melo Species 0.000 description 1
• 235000009842 Cucumis melo Nutrition 0.000 description 1
• 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
• 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
• 235000009849 Cucumis sativus Nutrition 0.000 description 1
• 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
• 241000219122 Cucurbita Species 0.000 description 1
• 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
• 241000219104 Cucurbitaceae Species 0.000 description 1
• CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
• 102000005927 Cysteine Proteases Human genes 0.000 description 1
• 108010005843 Cysteine Proteases Proteins 0.000 description 1
• FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
• FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
• FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
• 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
• ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 description 1
• ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 description 1
• 238000000018 DNA microarray Methods 0.000 description 1
• 101710118613 DNA polymerase sliding clamp 2 Proteins 0.000 description 1
• 239000003155 DNA primer Substances 0.000 description 1
• 230000033616 DNA repair Effects 0.000 description 1
• 238000001712 DNA sequencing Methods 0.000 description 1
• 241000208296 Datura Species 0.000 description 1
• 240000003421 Dianthus chinensis Species 0.000 description 1
• ZFIVKAOQEXOYFY-UHFFFAOYSA-N Diepoxybutane Chemical compound C1OC1C1OC1 ZFIVKAOQEXOYFY-UHFFFAOYSA-N 0.000 description 1
• 240000001879 Digitalis lutea Species 0.000 description 1
• 235000005903 Dioscorea Nutrition 0.000 description 1
• 244000281702 Dioscorea villosa Species 0.000 description 1
• 235000000504 Dioscorea villosa Nutrition 0.000 description 1
• 241000234272 Dioscoreaceae Species 0.000 description 1
• 241000512897 Elaeis Species 0.000 description 1
• 235000001942 Elaeis Nutrition 0.000 description 1
• 240000003133 Elaeis guineensis Species 0.000 description 1
• 235000001950 Elaeis guineensis Nutrition 0.000 description 1
• 241000218670 Ephedraceae Species 0.000 description 1
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
• 241001081474 Erythroxylaceae Species 0.000 description 1
• 241000735552 Erythroxylum Species 0.000 description 1
• 241000588724 Escherichia coli Species 0.000 description 1
• 108700039887 Essential Genes Proteins 0.000 description 1
• 239000005977 Ethylene Chemical group 0.000 description 1
• IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
• 241000221017 Euphorbiaceae Species 0.000 description 1
• 108091029865 Exogenous DNA Proteins 0.000 description 1
• 108700024394 Exon Proteins 0.000 description 1
• 108060002716 Exonuclease Proteins 0.000 description 1
• 108091060211 Expressed sequence tag Proteins 0.000 description 1
• 241000220485 Fabaceae Species 0.000 description 1
• 241000234643 Festuca arundinacea Species 0.000 description 1
• 235000016623 Fragaria vesca Nutrition 0.000 description 1
• 101710186901 Globulin 1 Proteins 0.000 description 1
• 102000053187 Glucuronidase Human genes 0.000 description 1
• 108010060309 Glucuronidase Proteins 0.000 description 1
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
• 108010070675 Glutathione transferase Proteins 0.000 description 1
• 102000005720 Glutathione transferase Human genes 0.000 description 1
• 239000004471 Glycine Substances 0.000 description 1
• 108700037728 Glycine max beta-conglycinin Proteins 0.000 description 1
• 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
• 108020005004 Guide RNA Proteins 0.000 description 1
• 241001316290 Gypsophila Species 0.000 description 1
• 108090001102 Hammerhead ribozyme Proteins 0.000 description 1
• 101710154606 Hemagglutinin Proteins 0.000 description 1
• 241000081543 Hesperis Species 0.000 description 1
• 235000015842 Hesperis Nutrition 0.000 description 1
• 244000043261 Hevea brasiliensis Species 0.000 description 1
• 241000282412 Homo Species 0.000 description 1
• 241000209219 Hordeum Species 0.000 description 1
• 241000208278 Hyoscyamus Species 0.000 description 1
• 108060003951 Immunoglobulin Proteins 0.000 description 1
• 241001048891 Jatropha curcas Species 0.000 description 1
• 108010009384 L-Iditol 2-Dehydrogenase Proteins 0.000 description 1
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
• SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical group C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
• 241000208822 Lactuca Species 0.000 description 1
• 235000003228 Lactuca sativa Nutrition 0.000 description 1
• 240000008415 Lactuca sativa Species 0.000 description 1
• 241000207923 Lamiaceae Species 0.000 description 1
• 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
• 102000016267 Leptin Human genes 0.000 description 1
• 108010092277 Leptin Proteins 0.000 description 1
• 108700005090 Lethal Genes Proteins 0.000 description 1
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
• 241000209510 Liliopsida Species 0.000 description 1
• 241000208202 Linaceae Species 0.000 description 1
• 241000208204 Linum Species 0.000 description 1
• 235000004431 Linum usitatissimum Nutrition 0.000 description 1
• 240000006240 Linum usitatissimum Species 0.000 description 1
• 241000724705 Lucerne transient streak virus Species 0.000 description 1
• 108060001084 Luciferase Proteins 0.000 description 1
• 239000005089 Luciferase Substances 0.000 description 1
• 241000227653 Lycopersicon Species 0.000 description 1
• 235000002262 Lycopersicon Nutrition 0.000 description 1
• 241000195948 Lycopodiaceae Species 0.000 description 1
• 241000195947 Lycopodium Species 0.000 description 1
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
• 239000004472 Lysine Substances 0.000 description 1
• GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
• 241000219071 Malvaceae Species 0.000 description 1
• 235000004456 Manihot esculenta Nutrition 0.000 description 1
• 101000763602 Manilkara zapota Thaumatin-like protein 1 Proteins 0.000 description 1
• 101000763586 Manilkara zapota Thaumatin-like protein 1a Proteins 0.000 description 1
• 229930195725 Mannitol Natural products 0.000 description 1
• 108020000290 Mannitol dehydrogenase Proteins 0.000 description 1
• 241000219823 Medicago Species 0.000 description 1
• 241000489991 Melanthiaceae Species 0.000 description 1
• 241001072983 Mentha Species 0.000 description 1
• 235000014435 Mentha Nutrition 0.000 description 1
• 241001074116 Miscanthus x giganteus Species 0.000 description 1
• 241000699670 Mus sp. Species 0.000 description 1
• 241000234295 Musa Species 0.000 description 1
• 101000966653 Musa acuminata Glucan endo-1,3-beta-glucosidase Proteins 0.000 description 1
• 240000005561 Musa balbisiana Species 0.000 description 1
• 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
• 241000234615 Musaceae Species 0.000 description 1
• 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
• 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
• 102000018463 Myo-Inositol-1-Phosphate Synthase Human genes 0.000 description 1
• 108091000020 Myo-Inositol-1-Phosphate Synthase Proteins 0.000 description 1
• 241000219926 Myrtaceae Species 0.000 description 1
• FUSGACRLAFQQRL-UHFFFAOYSA-N N-Ethyl-N-nitrosourea Chemical compound CCN(N=O)C(N)=O FUSGACRLAFQQRL-UHFFFAOYSA-N 0.000 description 1
• ZRKWMRDKSOPRRS-UHFFFAOYSA-N N-Methyl-N-nitrosourea Chemical compound O=NN(C)C(N)=O ZRKWMRDKSOPRRS-UHFFFAOYSA-N 0.000 description 1
• 102100034559 Natural resistance-associated macrophage protein 1 Human genes 0.000 description 1
• 206010028980 Neoplasm Diseases 0.000 description 1
• 241000250374 Nicotiana acaulis Species 0.000 description 1
• 244000061322 Nicotiana alata Species 0.000 description 1
• 241000250377 Nicotiana amplexicaulis Species 0.000 description 1
• 241001144490 Nicotiana arentsii Species 0.000 description 1
• 241000250375 Nicotiana benavidesii Species 0.000 description 1
• 241000207746 Nicotiana benthamiana Species 0.000 description 1
• 241001609967 Nicotiana clevelandii Species 0.000 description 1
• 241001244271 Nicotiana cordifolia Species 0.000 description 1
• 241001144496 Nicotiana corymbosa Species 0.000 description 1
• 241000208113 Nicotiana debneyi Species 0.000 description 1
• 241000862464 Nicotiana excelsior Species 0.000 description 1
• 244000006449 Nicotiana forgetiana Species 0.000 description 1
• 241000208128 Nicotiana glauca Species 0.000 description 1
• 241001495644 Nicotiana glutinosa Species 0.000 description 1
• 241001144503 Nicotiana goodspeedii Species 0.000 description 1
• 241000250366 Nicotiana gossei Species 0.000 description 1
• 241000579278 Nicotiana kawakamii Species 0.000 description 1
• 241000250368 Nicotiana knightiana Species 0.000 description 1
• 241000250019 Nicotiana langsdorffii Species 0.000 description 1
• 241000250027 Nicotiana linearis Species 0.000 description 1
• 241000250024 Nicotiana longiflora Species 0.000 description 1
• 241000250031 Nicotiana megalosiphon Species 0.000 description 1
• 241000250030 Nicotiana miersii Species 0.000 description 1
• 241000250041 Nicotiana noctiflora Species 0.000 description 1
• 241000228665 Nicotiana nudicaulis Species 0.000 description 1
• 241000208132 Nicotiana otophora Species 0.000 description 1
• 241000876839 Nicotiana paniculata Species 0.000 description 1
• 241001144492 Nicotiana pauciflora Species 0.000 description 1
• 241000250042 Nicotiana petunioides Species 0.000 description 1
• 241000208133 Nicotiana plumbaginifolia Species 0.000 description 1
• 241001144487 Nicotiana raimondii Species 0.000 description 1
• 241001290303 Nicotiana repanda Species 0.000 description 1
• 241001144498 Nicotiana rosulata subsp. ingulba Species 0.000 description 1
• 241001144486 Nicotiana rotundifolia Species 0.000 description 1
• 241000208134 Nicotiana rustica Species 0.000 description 1
• 241001144491 Nicotiana setchellii Species 0.000 description 1
• 241000250044 Nicotiana simulans Species 0.000 description 1
• 241000249970 Nicotiana solanifolia Species 0.000 description 1
• 241001144495 Nicotiana spegazzinii Species 0.000 description 1
• 241000249966 Nicotiana stocktonii Species 0.000 description 1
• 241001144480 Nicotiana suaveolens Species 0.000 description 1
• 241001144489 Nicotiana thyrsiflora Species 0.000 description 1
• 241000579280 Nicotiana tomentosa Species 0.000 description 1
• 241000249968 Nicotiana umbratica Species 0.000 description 1
• 241000228669 Nicotiana velutina Species 0.000 description 1
• 241001144494 Nicotiana wigandioides Species 0.000 description 1
• 108090000913 Nitrate Reductases Proteins 0.000 description 1
• MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical class O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 1
• 240000002061 Nothoscordum fragrans Species 0.000 description 1
• 241000209018 Nyssaceae Species 0.000 description 1
• 108010061618 O-succinylhomoserine (thiol)-lyase Proteins 0.000 description 1
• 241000123069 Ocyurus chrysurus Species 0.000 description 1
• 108700026244 Open Reading Frames Proteins 0.000 description 1
• 241000283973 Oryctolagus cuniculus Species 0.000 description 1
• 241000209094 Oryza Species 0.000 description 1
• 101710093908 Outer capsid protein VP4 Proteins 0.000 description 1
• 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 1
• 102000003697 P-type ATPases Human genes 0.000 description 1
• 108090000069 P-type ATPases Proteins 0.000 description 1
• 238000012408 PCR amplification Methods 0.000 description 1
• 241000209117 Panicum Species 0.000 description 1
• 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 description 1
• 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 description 1
• 241000218180 Papaveraceae Species 0.000 description 1
• 241000209046 Pennisetum Species 0.000 description 1
• 244000130556 Pennisetum purpureum Species 0.000 description 1
• 244000038248 Pennisetum spicatum Species 0.000 description 1
• 235000007195 Pennisetum typhoides Nutrition 0.000 description 1
• 108091093037 Peptide nucleic acid Proteins 0.000 description 1
• 244000081757 Phalaris arundinacea Species 0.000 description 1
• 101710163504 Phaseolin Proteins 0.000 description 1
• 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
• 244000046052 Phaseolus vulgaris Species 0.000 description 1
• 241000746983 Phleum pratense Species 0.000 description 1
• IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
• 108091000080 Phosphotransferase Proteins 0.000 description 1
• 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
• 244000082204 Phyllostachys viridis Species 0.000 description 1
• 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
• 241001250104 Physalis mottle virus Species 0.000 description 1
• BLUHKGOSFDHHGX-UHFFFAOYSA-N Phytol Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)C=CO BLUHKGOSFDHHGX-UHFFFAOYSA-N 0.000 description 1
• 241000218641 Pinaceae Species 0.000 description 1
• 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
• 241000018646 Pinus brutia Species 0.000 description 1
• 241000013557 Plantaginaceae Species 0.000 description 1
• 241000500437 Plutella xylostella Species 0.000 description 1
• 241000209048 Poa Species 0.000 description 1
• 241000209049 Poa pratensis Species 0.000 description 1
• 241000209504 Poaceae Species 0.000 description 1
• 239000002202 Polyethylene glycol Substances 0.000 description 1
• 241000161288 Populus candicans Species 0.000 description 1
• 235000011263 Populus tremuloides Nutrition 0.000 description 1
• 240000004923 Populus tremuloides Species 0.000 description 1
• ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 1
• 101710176177 Protein A56 Proteins 0.000 description 1
• 101150041925 RBCS gene Proteins 0.000 description 1
• 101150051143 RBCS1 gene Proteins 0.000 description 1
• 241000700159 Rattus Species 0.000 description 1
• 244000061121 Rauvolfia serpentina Species 0.000 description 1
• 102000006382 Ribonucleases Human genes 0.000 description 1
• 108010083644 Ribonucleases Proteins 0.000 description 1
• 108091028664 Ribonucleotide Proteins 0.000 description 1
• 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
• 235000003846 Ricinus Nutrition 0.000 description 1
• 241000322381 Ricinus <louse> Species 0.000 description 1
• 240000000528 Ricinus communis Species 0.000 description 1
• 235000004443 Ricinus communis Nutrition 0.000 description 1
• 241000220317 Rosa Species 0.000 description 1
• 235000004789 Rosa xanthina Nutrition 0.000 description 1
• 241000220222 Rosaceae Species 0.000 description 1
• 241001107098 Rubiaceae Species 0.000 description 1
• 108091006619 SLC11A1 Proteins 0.000 description 1
• 240000000111 Saccharum officinarum Species 0.000 description 1
• 235000007201 Saccharum officinarum Nutrition 0.000 description 1
• 241000746444 Saccharum sp. Species 0.000 description 1
• 241000218998 Salicaceae Species 0.000 description 1
• 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
• 241001093760 Sapindaceae Species 0.000 description 1
• 101100352756 Schizosaccharomyces pombe (strain 972 / ATCC 24843) pnu1 gene Proteins 0.000 description 1
• 101100528946 Schizosaccharomyces pombe (strain 972 / ATCC 24843) rpa1 gene Proteins 0.000 description 1
• 241000242873 Scopolia Species 0.000 description 1
• 241000209056 Secale Species 0.000 description 1
• 108010016634 Seed Storage Proteins Proteins 0.000 description 1
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
• 235000003434 Sesamum indicum Nutrition 0.000 description 1
• 244000040738 Sesamum orientale Species 0.000 description 1
• 108020004682 Single-Stranded DNA Proteins 0.000 description 1
• FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
• 241000208292 Solanaceae Species 0.000 description 1
• 235000002634 Solanum Nutrition 0.000 description 1
• 241000207763 Solanum Species 0.000 description 1
• 102100026974 Sorbitol dehydrogenase Human genes 0.000 description 1
• 235000015503 Sorghum bicolor subsp. drummondii Nutrition 0.000 description 1
• 241000746413 Spartina Species 0.000 description 1
• 241000219315 Spinacia Species 0.000 description 1
• 244000300264 Spinacia oleracea Species 0.000 description 1
• 235000009337 Spinacia oleracea Nutrition 0.000 description 1
• 241000923571 Sporobolus michauxianus Species 0.000 description 1
• 241000724703 Subterranean clover mottle virus Species 0.000 description 1
• 244000170625 Sudangrass Species 0.000 description 1
• 229940100389 Sulfonylurea Drugs 0.000 description 1
• 241000404542 Tanacetum Species 0.000 description 1
• 241001116495 Taxaceae Species 0.000 description 1
• 241001116500 Taxus Species 0.000 description 1
• HNZBNQYXWOLKBA-UHFFFAOYSA-N Tetrahydrofarnesol Natural products CC(C)CCCC(C)CCCC(C)=CCO HNZBNQYXWOLKBA-UHFFFAOYSA-N 0.000 description 1
• 235000006468 Thea sinensis Nutrition 0.000 description 1
• 241001122767 Theaceae Species 0.000 description 1
• 244000152045 Themeda triandra Species 0.000 description 1
• 102000002933 Thioredoxin Human genes 0.000 description 1
• 108010006368 Thioredoxin h Proteins 0.000 description 1
• 108091036066 Three prime untranslated region Proteins 0.000 description 1
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
• 239000004473 Threonine Substances 0.000 description 1
• 235000007303 Thymus vulgaris Nutrition 0.000 description 1
• 240000002657 Thymus vulgaris Species 0.000 description 1
• 241000723873 Tobacco mosaic virus Species 0.000 description 1
• 241000723677 Tobacco ringspot virus Species 0.000 description 1
• 108700009124 Transcription Initiation Site Proteins 0.000 description 1
• 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
• 241000209140 Triticum Species 0.000 description 1
• 102000004243 Tubulin Human genes 0.000 description 1
• 108090000704 Tubulin Proteins 0.000 description 1
• 108090000848 Ubiquitin Proteins 0.000 description 1
• 102000044159 Ubiquitin Human genes 0.000 description 1
• 238000010811 Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Methods 0.000 description 1
• 241000145124 Uniola Species 0.000 description 1
• 235000013419 Uniola paniculata Nutrition 0.000 description 1
• 240000007492 Uniola paniculata Species 0.000 description 1
• 102000039634 Untranslated RNA Human genes 0.000 description 1
• 108020004417 Untranslated RNA Proteins 0.000 description 1
• 241001002356 Valeriana edulis Species 0.000 description 1
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
• 241000724701 Velvet tobacco mottle virus Species 0.000 description 1
• 241000489523 Veratrum Species 0.000 description 1
• 241000863480 Vinca Species 0.000 description 1
• 241000726445 Viroids Species 0.000 description 1
• 241000219094 Vitaceae Species 0.000 description 1
• 235000009754 Vitis X bourquina Nutrition 0.000 description 1
• 235000012333 Vitis X labruscana Nutrition 0.000 description 1
• 235000014787 Vitis vinifera Nutrition 0.000 description 1
• 240000006365 Vitis vinifera Species 0.000 description 1
• 241000607479 Yersinia pestis Species 0.000 description 1
• 241000209149 Zea Species 0.000 description 1
• 235000007244 Zea mays Nutrition 0.000 description 1
• 101001036768 Zea mays Glucose-1-phosphate adenylyltransferase large subunit 1, chloroplastic/amyloplastic Proteins 0.000 description 1
• 101001040871 Zea mays Glutelin-2 Proteins 0.000 description 1
• 101000662549 Zea mays Sucrose synthase 1 Proteins 0.000 description 1
• FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
• 230000036579 abiotic stress Effects 0.000 description 1
• 230000009471 action Effects 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 239000004480 active ingredient Substances 0.000 description 1
• 210000005006 adaptive immune system Anatomy 0.000 description 1
• 238000001261 affinity purification Methods 0.000 description 1
• 230000009418 agronomic effect Effects 0.000 description 1
• 235000004279 alanine Nutrition 0.000 description 1
• 150000001299 aldehydes Chemical class 0.000 description 1
• PPQRONHOSHZGFQ-LMVFSUKVSA-N aldehydo-D-ribose 5-phosphate Chemical group OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PPQRONHOSHZGFQ-LMVFSUKVSA-N 0.000 description 1
• BOTWFXYSPFMFNR-OALUTQOASA-N all-rac-phytol Natural products CC(C)CCC[C@H](C)CCC[C@H](C)CCCC(C)=CCO BOTWFXYSPFMFNR-OALUTQOASA-N 0.000 description 1
• 231100000788 altered fertility Toxicity 0.000 description 1
• WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
• 125000000539 amino acid group Chemical group 0.000 description 1
• 229910021529 ammonia Inorganic materials 0.000 description 1
• 229930014345 anabasine Natural products 0.000 description 1
• 239000003242 anti bacterial agent Substances 0.000 description 1
• 239000000427 antigen Substances 0.000 description 1
• 108091007433 antigens Proteins 0.000 description 1
• 102000036639 antigens Human genes 0.000 description 1
• 229940045686 antimetabolites antineoplastic purine analogs Drugs 0.000 description 1
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
• 101150037081 aroA gene Proteins 0.000 description 1
• 238000003491 array Methods 0.000 description 1
• 244000030166 artemisia Species 0.000 description 1
• 235000009052 artemisia Nutrition 0.000 description 1
• 210000004436 artificial bacterial chromosome Anatomy 0.000 description 1
• 210000001106 artificial yeast chromosome Anatomy 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• 235000003704 aspartic acid Nutrition 0.000 description 1
• QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
• MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 description 1
• 229940097012 bacillus thuringiensis Drugs 0.000 description 1
• 239000011425 bamboo Substances 0.000 description 1
• 239000011324 bead Substances 0.000 description 1
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
• 238000002306 biochemical method Methods 0.000 description 1
• 239000003139 biocide Substances 0.000 description 1
• QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 1
• 229960001561 bleomycin Drugs 0.000 description 1
• OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
• 230000000903 blocking effect Effects 0.000 description 1
• 102200024645 c.104G>A Human genes 0.000 description 1
• 102220367873 c.267G>A Human genes 0.000 description 1
• 238000004364 calculation method Methods 0.000 description 1
• 201000011510 cancer Diseases 0.000 description 1
• 239000001390 capsicum minimum Substances 0.000 description 1
• 239000002775 capsule Substances 0.000 description 1
• 150000001720 carbohydrates Chemical class 0.000 description 1
• 235000014633 carbohydrates Nutrition 0.000 description 1
• 229910052799 carbon Inorganic materials 0.000 description 1
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
• 231100000260 carcinogenicity Toxicity 0.000 description 1
• 230000007670 carcinogenicity Effects 0.000 description 1
• 210000005056 cell body Anatomy 0.000 description 1
• 210000000170 cell membrane Anatomy 0.000 description 1
• 210000002421 cell wall Anatomy 0.000 description 1
• 230000004700 cellular uptake Effects 0.000 description 1
• 108010040093 cellulose synthase Proteins 0.000 description 1
• 230000008859 change Effects 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 125000003636 chemical group Chemical group 0.000 description 1
• 230000001055 chewing effect Effects 0.000 description 1
• JCKYGMPEJWAADB-UHFFFAOYSA-N chlorambucil Chemical compound OC(=O)CCCC1=CC=C(N(CCCl)CCCl)C=C1 JCKYGMPEJWAADB-UHFFFAOYSA-N 0.000 description 1
• 229960004630 chlorambucil Drugs 0.000 description 1
• 210000003763 chloroplast Anatomy 0.000 description 1
• VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
• 230000008711 chromosomal rearrangement Effects 0.000 description 1
• 235000019506 cigar Nutrition 0.000 description 1
• 230000002060 circadian Effects 0.000 description 1
• 229920003211 cis-1,4-polyisoprene Polymers 0.000 description 1
• 238000010367 cloning Methods 0.000 description 1
• 230000008645 cold stress Effects 0.000 description 1
• 235000018597 common camellia Nutrition 0.000 description 1
• 230000001143 conditioned effect Effects 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 239000008358 core component Substances 0.000 description 1
• 230000002596 correlated effect Effects 0.000 description 1
• 230000010154 cross-pollination Effects 0.000 description 1
• 210000004748 cultured cell Anatomy 0.000 description 1
• 229960004397 cyclophosphamide Drugs 0.000 description 1
• 230000002559 cytogenic effect Effects 0.000 description 1
• UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
• 239000004062 cytokinin Substances 0.000 description 1
• 229940104302 cytosine Drugs 0.000 description 1
• 230000001086 cytosolic effect Effects 0.000 description 1
• 230000006378 damage Effects 0.000 description 1
• 230000034994 death Effects 0.000 description 1
• 230000003111 delayed effect Effects 0.000 description 1
• 239000005547 deoxyribonucleotide Substances 0.000 description 1
• 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
• DENRZWYUOJLTMF-UHFFFAOYSA-N diethyl sulfate Chemical compound CCOS(=O)(=O)OCC DENRZWYUOJLTMF-UHFFFAOYSA-N 0.000 description 1
• 229940008406 diethyl sulfate Drugs 0.000 description 1
• 238000009792 diffusion process Methods 0.000 description 1
• 235000004879 dioscorea Nutrition 0.000 description 1
• 239000001177 diphosphate Substances 0.000 description 1
• XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
• 235000011180 diphosphates Nutrition 0.000 description 1
• 201000010099 disease Diseases 0.000 description 1
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
• 238000009826 distribution Methods 0.000 description 1
• 239000003814 drug Substances 0.000 description 1
• 229940079593 drug Drugs 0.000 description 1
• 241001493065 dsRNA viruses Species 0.000 description 1
• 238000005485 electric heating Methods 0.000 description 1
• 239000012149 elution buffer Substances 0.000 description 1
• 210000002257 embryonic structure Anatomy 0.000 description 1
• 239000000839 emulsion Substances 0.000 description 1
• 108010050663 endodeoxyribonuclease CreI Proteins 0.000 description 1
• 230000007613 environmental effect Effects 0.000 description 1
• 238000007824 enzymatic assay Methods 0.000 description 1
• 230000007515 enzymatic degradation Effects 0.000 description 1
• 238000006911 enzymatic reaction Methods 0.000 description 1
• 238000001952 enzyme assay Methods 0.000 description 1
• 210000002919 epithelial cell Anatomy 0.000 description 1
• 102000013165 exonuclease Human genes 0.000 description 1
• 239000004744 fabric Substances 0.000 description 1
• 230000002349 favourable effect Effects 0.000 description 1
• 230000035558 fertility Effects 0.000 description 1
• 239000003337 fertilizer Substances 0.000 description 1
• 239000000945 filler Substances 0.000 description 1
• 239000010408 film Substances 0.000 description 1
• 239000000796 flavoring agent Substances 0.000 description 1
• 235000019634 flavors Nutrition 0.000 description 1
• 235000004426 flaxseed Nutrition 0.000 description 1
• 230000008124 floral development Effects 0.000 description 1
• 239000006260 foam Substances 0.000 description 1
• 235000013305 food Nutrition 0.000 description 1
• 239000000446 fuel Substances 0.000 description 1
• 108020001507 fusion proteins Proteins 0.000 description 1
• 102000037865 fusion proteins Human genes 0.000 description 1
• 239000000499 gel Substances 0.000 description 1
• 238000001502 gel electrophoresis Methods 0.000 description 1
• 238000012226 gene silencing method Methods 0.000 description 1
• 102000034356 gene-regulatory proteins Human genes 0.000 description 1
• 108091006104 gene-regulatory proteins Proteins 0.000 description 1
• 102000054766 genetic haplotypes Human genes 0.000 description 1
• 210000004602 germ cell Anatomy 0.000 description 1
• 230000035784 germination Effects 0.000 description 1
• IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
• 235000013922 glutamic acid Nutrition 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
• 239000008187 granular material Substances 0.000 description 1
• 230000012010 growth Effects 0.000 description 1
• 239000001963 growth medium Substances 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 239000000185 hemagglutinin Substances 0.000 description 1
• GNOIPBMMFNIUFM-UHFFFAOYSA-N hexamethylphosphoric triamide Chemical compound CN(C)P(=O)(N(C)C)N(C)C GNOIPBMMFNIUFM-UHFFFAOYSA-N 0.000 description 1
• 238000013537 high throughput screening Methods 0.000 description 1
• 238000002744 homologous recombination Methods 0.000 description 1
• 230000006801 homologous recombination Effects 0.000 description 1
• 210000004754 hybrid cell Anatomy 0.000 description 1
• 239000001257 hydrogen Substances 0.000 description 1
• 229910052739 hydrogen Inorganic materials 0.000 description 1
• 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
• 238000005286 illumination Methods 0.000 description 1
• 230000028993 immune response Effects 0.000 description 1
• 230000005847 immunogenicity Effects 0.000 description 1
• 102000018358 immunoglobulin Human genes 0.000 description 1
• 238000001114 immunoprecipitation Methods 0.000 description 1
• 238000012744 immunostaining Methods 0.000 description 1
• 239000000411 inducer Substances 0.000 description 1
• 230000006698 induction Effects 0.000 description 1
• 208000015181 infectious disease Diseases 0.000 description 1
• 239000004615 ingredient Substances 0.000 description 1
• 238000002347 injection Methods 0.000 description 1
• 239000007924 injection Substances 0.000 description 1
• 229910052500 inorganic mineral Inorganic materials 0.000 description 1
• 239000000543 intermediate Substances 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 230000005865 ionizing radiation Effects 0.000 description 1
• 229960000310 isoleucine Drugs 0.000 description 1
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
• 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
• 235000021374 legumes Nutrition 0.000 description 1
• 230000003902 lesion Effects 0.000 description 1
• 125000005647 linker group Chemical group 0.000 description 1
• 239000002502 liposome Substances 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 238000004811 liquid chromatography Methods 0.000 description 1
• 210000002540 macrophage Anatomy 0.000 description 1
• 238000012423 maintenance Methods 0.000 description 1
• 235000005739 manihot Nutrition 0.000 description 1
• 239000000594 mannitol Substances 0.000 description 1
• 235000010355 mannitol Nutrition 0.000 description 1
• 230000013011 mating Effects 0.000 description 1
• 230000035800 maturation Effects 0.000 description 1
• 229960004961 mechlorethamine Drugs 0.000 description 1
• HAWPXGHAZFHHAD-UHFFFAOYSA-N mechlorethamine Chemical class ClCCN(C)CCCl HAWPXGHAZFHHAD-UHFFFAOYSA-N 0.000 description 1
• 239000002609 medium Substances 0.000 description 1
• 229960001924 melphalan Drugs 0.000 description 1
• SGDBTWWWUNNDEQ-LBPRGKRZSA-N melphalan Chemical compound OC(=O)[C@@H](N)CC1=CC=C(N(CCCl)CCCl)C=C1 SGDBTWWWUNNDEQ-LBPRGKRZSA-N 0.000 description 1
• 210000000473 mesophyll cell Anatomy 0.000 description 1
• 229910052751 metal Inorganic materials 0.000 description 1
• 239000002184 metal Substances 0.000 description 1
• 229910021645 metal ion Inorganic materials 0.000 description 1
• RXMBKOPBFXCPDD-UHFFFAOYSA-N methoxyphosphonamidous acid Chemical group COP(N)O RXMBKOPBFXCPDD-UHFFFAOYSA-N 0.000 description 1
• MBABOKRGFJTBAE-UHFFFAOYSA-N methyl methanesulfonate Chemical compound COS(C)(=O)=O MBABOKRGFJTBAE-UHFFFAOYSA-N 0.000 description 1
• 230000011987 methylation Effects 0.000 description 1
• 238000007069 methylation reaction Methods 0.000 description 1
• 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 1
• 238000002493 microarray Methods 0.000 description 1
• 239000011707 mineral Substances 0.000 description 1
• 238000001823 molecular biology technique Methods 0.000 description 1
• 125000004573 morpholin-4-yl group Chemical group N1(CCOCC1)* 0.000 description 1
• 230000036457 multidrug resistance Effects 0.000 description 1
• 235000010460 mustard Nutrition 0.000 description 1
• ZUHZZVMEUAUWHY-UHFFFAOYSA-N n,n-dimethylpropan-1-amine Chemical compound CCCN(C)C ZUHZZVMEUAUWHY-UHFFFAOYSA-N 0.000 description 1
• 239000006225 natural substrate Substances 0.000 description 1
• 230000007935 neutral effect Effects 0.000 description 1
• QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
• 108010058731 nopaline synthase Proteins 0.000 description 1
• 238000007899 nucleic acid hybridization Methods 0.000 description 1
• 235000015097 nutrients Nutrition 0.000 description 1
• 239000002751 oligonucleotide probe Substances 0.000 description 1
• 238000010397 one-hybrid screening Methods 0.000 description 1
• 150000002894 organic compounds Chemical class 0.000 description 1
• 230000003647 oxidation Effects 0.000 description 1
• 238000007254 oxidation reaction Methods 0.000 description 1
• 238000004806 packaging method and process Methods 0.000 description 1
• 230000001717 pathogenic effect Effects 0.000 description 1
• 230000007918 pathogenicity Effects 0.000 description 1
• 230000037361 pathway Effects 0.000 description 1
• 238000002823 phage display Methods 0.000 description 1
• LWTDZKXXJRRKDG-UHFFFAOYSA-N phaseollin Natural products C1OC2=CC(O)=CC=C2C2C1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-UHFFFAOYSA-N 0.000 description 1
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
• 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
• 102000020233 phosphotransferase Human genes 0.000 description 1
• 230000029553 photosynthesis Effects 0.000 description 1
• 238000010672 photosynthesis Methods 0.000 description 1
• 230000000243 photosynthetic effect Effects 0.000 description 1
• 238000000053 physical method Methods 0.000 description 1
• BOTWFXYSPFMFNR-PYDDKJGSSA-N phytol Chemical compound CC(C)CCC[C@@H](C)CCC[C@@H](C)CCC\C(C)=C\CO BOTWFXYSPFMFNR-PYDDKJGSSA-N 0.000 description 1
• 239000001739 pinus spp. Substances 0.000 description 1
• 210000000745 plant chromosome Anatomy 0.000 description 1
• 239000013600 plasmid vector Substances 0.000 description 1
• 229920001983 poloxamer Polymers 0.000 description 1
• 230000008488 polyadenylation Effects 0.000 description 1
• 229920000447 polyanionic polymer Polymers 0.000 description 1
• 229920001223 polyethylene glycol Polymers 0.000 description 1
• 229920002704 polyhistidine Polymers 0.000 description 1
• 229920005862 polyol Polymers 0.000 description 1
• 150000003077 polyols Chemical class 0.000 description 1
• 239000000843 powder Substances 0.000 description 1
• 239000002243 precursor Substances 0.000 description 1
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
• CPTBDICYNRMXFX-UHFFFAOYSA-N procarbazine Chemical compound CNNCC1=CC=C(C(=O)NC(C)C)C=C1 CPTBDICYNRMXFX-UHFFFAOYSA-N 0.000 description 1
• 229960000624 procarbazine Drugs 0.000 description 1
• WGYKZJWCGVVSQN-UHFFFAOYSA-O propan-1-aminium Chemical compound CCC[NH3+] WGYKZJWCGVVSQN-UHFFFAOYSA-O 0.000 description 1
• 230000004952 protein activity Effects 0.000 description 1
• 235000015136 pumpkin Nutrition 0.000 description 1
• 150000003212 purines Chemical class 0.000 description 1
• UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
• 238000010791 quenching Methods 0.000 description 1
• JETDZFFCRPFPDH-UHFFFAOYSA-N quinacrine mustard dihydrochloride Chemical compound [H+].[H+].[Cl-].[Cl-].C1=C(Cl)C=CC2=C(NC(C)CCCN(CCCl)CCCl)C3=CC(OC)=CC=C3N=C21 JETDZFFCRPFPDH-UHFFFAOYSA-N 0.000 description 1
• 230000008707 rearrangement Effects 0.000 description 1
• 230000005849 recognition of pollen Effects 0.000 description 1
• 238000005215 recombination Methods 0.000 description 1
• 238000011084 recovery Methods 0.000 description 1
• 230000014493 regulation of gene expression Effects 0.000 description 1
• 230000008960 regulation of mRNA stability Effects 0.000 description 1
• 230000020236 regulation of protein stability Effects 0.000 description 1
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
• 230000008439 repair process Effects 0.000 description 1
• 239000011347 resin Substances 0.000 description 1
• 229920005989 resin Polymers 0.000 description 1
• 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
• 230000000717 retained effect Effects 0.000 description 1
• 238000004007 reversed phase HPLC Methods 0.000 description 1
• 239000002336 ribonucleotide Substances 0.000 description 1
• 125000002652 ribonucleotide group Chemical group 0.000 description 1
• 235000009566 rice Nutrition 0.000 description 1
• 230000021749 root development Effects 0.000 description 1
• 230000002786 root growth Effects 0.000 description 1
• 102220236172 rs1131691995 Human genes 0.000 description 1
• 102220335613 rs1553410728 Human genes 0.000 description 1
• 102220316985 rs1553640288 Human genes 0.000 description 1
• 102220279097 rs1554656344 Human genes 0.000 description 1
• 102220295344 rs1555894553 Human genes 0.000 description 1
• 102200079919 rs193302886 Human genes 0.000 description 1
• 102220244330 rs368413008 Human genes 0.000 description 1
• 102220139813 rs372921526 Human genes 0.000 description 1
• 102200144905 rs397514763 Human genes 0.000 description 1
• 102200058227 rs773814837 Human genes 0.000 description 1
• 102220021416 rs80357110 Human genes 0.000 description 1
• 102220104592 rs879254078 Human genes 0.000 description 1
• 235000012420 sanguinaria Nutrition 0.000 description 1
• 230000028327 secretion Effects 0.000 description 1
• 230000008117 seed development Effects 0.000 description 1
• 238000010187 selection method Methods 0.000 description 1
• 230000009758 senescence Effects 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 239000002002 slurry Substances 0.000 description 1
• 229910001415 sodium ion Inorganic materials 0.000 description 1
• 239000001488 sodium phosphate Substances 0.000 description 1
• 229910000162 sodium phosphate Inorganic materials 0.000 description 1
• 239000002689 soil Substances 0.000 description 1
• 239000007787 solid Substances 0.000 description 1
• 239000007790 solid phase Substances 0.000 description 1
• 239000000600 sorbitol Substances 0.000 description 1
• 108010048090 soybean lectin Proteins 0.000 description 1
• 235000020354 squash Nutrition 0.000 description 1
• 238000010186 staining Methods 0.000 description 1
• 230000003068 static effect Effects 0.000 description 1
• 238000007619 statistical method Methods 0.000 description 1
• 230000014793 stomatal movement Effects 0.000 description 1
• 238000003860 storage Methods 0.000 description 1
• 150000003871 sulfonates Chemical class 0.000 description 1
• YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 239000004094 surface-active agent Substances 0.000 description 1
• 238000000672 surface-enhanced laser desorption--ionisation Methods 0.000 description 1
• 230000008685 targeting Effects 0.000 description 1
• 238000012225 targeting induced local lesions in genomes Methods 0.000 description 1
• 231100000378 teratogenic Toxicity 0.000 description 1
• 230000003390 teratogenic effect Effects 0.000 description 1
• RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical class [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
• 108060008226 thioredoxin Proteins 0.000 description 1
• 229940094937 thioredoxin Drugs 0.000 description 1
• 239000001585 thymus vulgaris Substances 0.000 description 1
• 239000003053 toxin Substances 0.000 description 1
• 231100000765 toxin Toxicity 0.000 description 1
• 238000011426 transformation method Methods 0.000 description 1
• 230000007704 transition Effects 0.000 description 1
• 238000002054 transplantation Methods 0.000 description 1
• 230000001960 triggered effect Effects 0.000 description 1
• RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
• 230000005074 turgor pressure Effects 0.000 description 1
• 238000010396 two-hybrid screening Methods 0.000 description 1
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
• 238000001195 ultra high performance liquid chromatography Methods 0.000 description 1
• 241000701447 unidentified baculovirus Species 0.000 description 1
• 241001430294 unidentified retrovirus Species 0.000 description 1
• 239000004474 valine Substances 0.000 description 1
• 238000007740 vapor deposition Methods 0.000 description 1
• 239000003981 vehicle Substances 0.000 description 1
• 229940057613 veratrum Drugs 0.000 description 1
• OGWKCGZFUXNPDA-XQKSVPLYSA-N vincristine Chemical compound C([N@]1C[C@@H](C[C@]2(C(=O)OC)C=3C(=CC4=C([C@]56[C@H]([C@@]([C@H](OC(C)=O)[C@]7(CC)C=CCN([C@H]67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)C[C@@](C1)(O)CC)CC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-XQKSVPLYSA-N 0.000 description 1
• 229960004528 vincristine Drugs 0.000 description 1
• OGWKCGZFUXNPDA-UHFFFAOYSA-N vincristine Natural products C1C(CC)(O)CC(CC2(C(=O)OC)C=3C(=CC4=C(C56C(C(C(OC(C)=O)C7(CC)C=CCN(C67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)CN1CCC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-UHFFFAOYSA-N 0.000 description 1
• 238000011179 visual inspection Methods 0.000 description 1
• 239000011534 wash buffer Substances 0.000 description 1
• 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 1