CA2894955C - Tobacco specific nitrosamine reduction in plants - Google Patents

Tobacco specific nitrosamine reduction in plants Download PDF

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Publication number
CA2894955C
CA2894955C CA2894955A CA2894955A CA2894955C CA 2894955 C CA2894955 C CA 2894955C CA 2894955 A CA2894955 A CA 2894955A CA 2894955 A CA2894955 A CA 2894955A CA 2894955 C CA2894955 C CA 2894955C
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CA
Canada
Prior art keywords
seq
plant
mutant
plants
sequence
Prior art date
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Active
Application number
CA2894955A
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English (en)
French (fr)
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CA2894955A1 (en
Inventor
Lucien Bovet
Prisca Campanoni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Philip Morris Products SA
Original Assignee
Philip Morris Products SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products SA filed Critical Philip Morris Products SA
Publication of CA2894955A1 publication Critical patent/CA2894955A1/en
Application granted granted Critical
Publication of CA2894955C publication Critical patent/CA2894955C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241—Extraction of specific substances
    • A24B15/245—Nitrosamines
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Manufacture Of Tobacco Products (AREA)
CA2894955A 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants Active CA2894955C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12198966 2012-12-21
EP12198966.9 2012-12-21
PCT/EP2013/077532 WO2014096283A2 (en) 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants

Publications (2)

Publication Number Publication Date
CA2894955A1 CA2894955A1 (en) 2014-06-26
CA2894955C true CA2894955C (en) 2023-10-31

Family

ID=47559184

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2894955A Active CA2894955C (en) 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants

Country Status (11)

Country Link
US (1) US10563215B2 (enExample)
EP (1) EP2935588B1 (enExample)
JP (1) JP6693747B2 (enExample)
KR (1) KR102253223B1 (enExample)
CN (1) CN105247053B (enExample)
AP (1) AP2015008563A0 (enExample)
CA (1) CA2894955C (enExample)
MX (2) MX2015008084A (enExample)
PH (1) PH12015501204B1 (enExample)
RU (1) RU2735254C2 (enExample)
WO (1) WO2014096283A2 (enExample)

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US9980509B2 (en) 2013-04-05 2018-05-29 R.J. Reynolds Tobacco Company Modification of bacterial profile of tobacco
EP3160988A2 (en) * 2014-06-25 2017-05-03 Philip Morris Products S.A. Modulation of biomass in plants by ectopic expression of a chloride channel
GB201501941D0 (en) * 2015-02-05 2015-03-25 British American Tobacco Co Method
HK1250883A1 (zh) * 2015-05-05 2019-01-18 North Carolina State University 减少烟草中的烟草特异性亚硝胺nnk的方法和组合物
WO2017156535A1 (en) * 2016-03-11 2017-09-14 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having reduced or eliminated suckers
JP2020529206A (ja) 2017-07-31 2020-10-08 アール・ジエイ・レイノルズ・タバコ・カンパニー 植物におけるウイルスベースの遺伝子編集のための方法及び組成物
WO2019185699A1 (en) 2018-03-28 2019-10-03 Philip Morris Products S.A. Modulating reducing sugar content in a plant
KR102995073B1 (ko) 2018-03-28 2026-07-24 필립모리스 프로덕츠 에스.에이. 식물 내 아미노산 함량 조절
GB201812603D0 (en) 2018-08-02 2018-09-19 British American Tobacco Investments Ltd Method
EP3902390A1 (en) * 2018-12-30 2021-11-03 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
WO2020247821A2 (en) * 2019-06-05 2020-12-10 R.J. Reynolds Tobacco Company bZIP TRANSCRIPTION FACTORS REGULATE CONVERSION OF NICOTINE TO NORNICOTINE AND REDUCE LEVELS OF TOBACCO SPECIFIC (TSNA) PRECURSORS
CN110499388B (zh) * 2019-09-29 2023-03-24 云南省烟草农业科学研究院 鉴别烟草抗斑萎病位点rtsw等位基因类型的共显性标记引物组、鉴别方法及其应用
WO2021063863A1 (en) 2019-10-01 2021-04-08 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (sultr3)
KR20220070305A (ko) 2019-10-01 2022-05-30 필립모리스 프로덕츠 에스.에이. 식물 내 환원당 (inv) 함량 조절
CN111690661A (zh) * 2020-06-01 2020-09-22 云南省烟草农业科学研究院 一种烟草NtbHLH13基因突变体及分子鉴定方法和应用
US20240368615A1 (en) 2021-09-10 2024-11-07 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
US20250084427A1 (en) 2021-12-21 2025-03-13 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN118475234A (zh) * 2021-12-27 2024-08-09 日本烟草产业株式会社 烟草植物
KR20250078994A (ko) 2022-10-13 2025-06-04 필립모리스 프로덕츠 에스.에이. Ntp2 조절에 의한 잎 바이오매스 및 질소 사용 효율 증가
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
WO2024160860A1 (en) 2023-02-02 2024-08-08 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
CN117025626B (zh) * 2023-06-29 2025-02-14 中国烟草总公司郑州烟草研究院 烟草硝酸盐转运蛋白NtNPF7.4及其编码基因、基因编辑载体和应用
CN117025627B (zh) * 2023-06-29 2024-11-01 中国烟草总公司郑州烟草研究院 烟草氯离子通道蛋白NtCLC13及其编码基因和应用
CN120944912B (zh) * 2025-09-17 2026-05-08 河南省农业科学院烟草研究所 NtGCN1基因及其在调控烟叶氯离子含量中的应用

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US5810020A (en) 1993-09-07 1998-09-22 Osmotek, Inc. Process for removing nitrogen-containing anions and tobacco-specific nitrosamines from tobacco products
US6202649B1 (en) 1996-12-02 2001-03-20 Regent Court Technologies Method of treating tobacco to reduce nitrosamine content, and products produced thereby
US6586661B1 (en) * 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
EE9900590A (et) 1997-06-20 2000-08-15 Regent Court Technologies Nitrosamiinide sisaldust vähendav tubaka töötlemise meetod ja seejuures saadavad tooted
US7700834B2 (en) * 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
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DK1623634T3 (da) * 2003-05-06 2013-07-15 Japan Tobacco Inc Fremgangsmåde til fremstilling af regenereret tobaksmateriale
JP4160868B2 (ja) * 2003-07-10 2008-10-08 日本たばこ産業株式会社 葉タバコ中のニトロソアミン含量の低減方法
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ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
AU2007329776B2 (en) 2006-10-13 2013-05-16 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
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AP3226A (en) 2007-12-13 2015-04-30 Philip Morris Products Sa Transgenic plants modifier for reducede cadmium transport, derivative products, and related methods
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KR20120126061A (ko) * 2009-10-22 2012-11-20 재단법인 작물유전체기능연구사업단 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법
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Also Published As

Publication number Publication date
PH12015501204A1 (en) 2015-08-17
KR102253223B1 (ko) 2021-05-21
AP2015008563A0 (en) 2015-06-30
CN105247053A (zh) 2016-01-13
JP6693747B2 (ja) 2020-05-13
WO2014096283A2 (en) 2014-06-26
EP2935588B1 (en) 2026-03-25
MX2019001598A (es) 2019-07-01
US20150315603A1 (en) 2015-11-05
RU2015126909A (ru) 2017-01-27
MX2015008084A (es) 2016-03-31
PH12015501204B1 (en) 2022-07-15
CA2894955A1 (en) 2014-06-26
EP2935588C0 (en) 2026-03-25
RU2735254C2 (ru) 2020-10-29
US10563215B2 (en) 2020-02-18
JP2016508032A (ja) 2016-03-17
KR20150096451A (ko) 2015-08-24
CN105247053B (zh) 2020-07-31
EP2935588A2 (en) 2015-10-28
WO2014096283A3 (en) 2014-08-14

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