CA2894955C - Tobacco specific nitrosamine reduction in plants - Google Patents

Tobacco specific nitrosamine reduction in plants Download PDF

Info

Publication number
CA2894955C
CA2894955C CA2894955A CA2894955A CA2894955C CA 2894955 C CA2894955 C CA 2894955C CA 2894955 A CA2894955 A CA 2894955A CA 2894955 A CA2894955 A CA 2894955A CA 2894955 C CA2894955 C CA 2894955C
Authority
CA
Canada
Prior art keywords
seq
plant
mutant
plants
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2894955A
Other languages
English (en)
French (fr)
Other versions
CA2894955A1 (en
Inventor
Lucien Bovet
Prisca Campanoni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Philip Morris Products SA
Original Assignee
Philip Morris Products SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products SA filed Critical Philip Morris Products SA
Publication of CA2894955A1 publication Critical patent/CA2894955A1/en
Application granted granted Critical
Publication of CA2894955C publication Critical patent/CA2894955C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241—Extraction of specific substances
    • A24B15/245—Nitrosamines
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Manufacture Of Tobacco Products (AREA)
CA2894955A 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants Active CA2894955C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12198966 2012-12-21
EP12198966.9 2012-12-21
PCT/EP2013/077532 WO2014096283A2 (en) 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants

Publications (2)

Publication Number Publication Date
CA2894955A1 CA2894955A1 (en) 2014-06-26
CA2894955C true CA2894955C (en) 2023-10-31

Family

ID=47559184

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2894955A Active CA2894955C (en) 2012-12-21 2013-12-19 Tobacco specific nitrosamine reduction in plants

Country Status (11)

Country Link
US (1) US10563215B2 (enExample)
EP (1) EP2935588A2 (enExample)
JP (1) JP6693747B2 (enExample)
KR (1) KR102253223B1 (enExample)
CN (1) CN105247053B (enExample)
AP (1) AP2015008563A0 (enExample)
CA (1) CA2894955C (enExample)
MX (2) MX2015008084A (enExample)
PH (1) PH12015501204B1 (enExample)
RU (1) RU2735254C2 (enExample)
WO (1) WO2014096283A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9980509B2 (en) 2013-04-05 2018-05-29 R.J. Reynolds Tobacco Company Modification of bacterial profile of tobacco
US20170145431A1 (en) * 2014-06-25 2017-05-25 Philip Morris Products S.A. Modulation of nitrate content in plants
GB201501941D0 (en) * 2015-02-05 2015-03-25 British American Tobacco Co Method
BR112017023719A2 (pt) * 2015-05-05 2018-07-17 Univ North Carolina State planta de tabaco, parte de planta e/ou célula de planta, semente, cultura de planta, produto de tabaco, método para reduzir pon e/ou nnk, método de produção de uma planta e método para produzir um produto de tabaco
CN117089525A (zh) * 2016-03-11 2023-11-21 奥驰亚客户服务有限公司 用于生产烟杈减少或消除的烟草植物和制品的组合物和方法
EP3661354A1 (en) 2017-07-31 2020-06-10 R. J. Reynolds Tobacco Company Methods and compositions for viral-based gene editing in plants
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
BR112020017430A2 (pt) 2018-03-28 2021-01-19 Philip Morris Products S.A. Modulação do teor de açúcar redutor em uma planta
CN113271768B (zh) * 2018-12-30 2024-02-06 菲利普莫里斯生产公司 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
EP3979839A4 (en) * 2019-06-05 2023-06-28 R. J. Reynolds Tobacco Company Bzip transcription factors regulate conversion of nicotine to nornicotine and reduce levels of tobacco specific (tsna) precursors
CN110499388B (zh) * 2019-09-29 2023-03-24 云南省烟草农业科学研究院 鉴别烟草抗斑萎病位点rtsw等位基因类型的共显性标记引物组、鉴别方法及其应用
BR112022005552A2 (pt) 2019-10-01 2022-06-28 Philip Morris Products Sa Modulação da redução do teor de açúcar em uma planta (inv)
US12419264B2 (en) 2019-10-01 2025-09-23 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (SULTR3)
CN111690661A (zh) * 2020-06-01 2020-09-22 云南省烟草农业科学研究院 一种烟草NtbHLH13基因突变体及分子鉴定方法和应用
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
EP4458140A4 (en) * 2021-12-27 2025-10-15 Japan Tobacco Inc TOBACCO PLANT
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ｎｔｐ２を調節することによって葉バイオマスおよび窒素利用効率を増加させる
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
CN120476136A (zh) 2023-02-02 2025-08-12 菲利普莫里斯生产公司 编码赖氨酸酮戊二酸还原酶的基因的调节
CN117025627B (zh) * 2023-06-29 2024-11-01 中国烟草总公司郑州烟草研究院 烟草氯离子通道蛋白NtCLC13及其编码基因和应用
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
CN117025626B (zh) * 2023-06-29 2025-02-14 中国烟草总公司郑州烟草研究院 烟草硝酸盐转运蛋白NtNPF7.4及其编码基因、基因编辑载体和应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5810020A (en) 1993-09-07 1998-09-22 Osmotek, Inc. Process for removing nitrogen-containing anions and tobacco-specific nitrosamines from tobacco products
US6202649B1 (en) 1996-12-02 2001-03-20 Regent Court Technologies Method of treating tobacco to reduce nitrosamine content, and products produced thereby
US6586661B1 (en) * 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
IL154166A (en) 1997-06-20 2005-09-25 Regent Court Technologies Tobacco products having low nitrosamine content
US7700834B2 (en) * 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
RU2191529C1 (ru) 2001-12-21 2002-10-27 Романенко Александр Павлович Устройство для курения
RU2310353C2 (ru) * 2003-05-06 2007-11-20 Джапан Тобакко Инк. Способ производства восстановленного табачного материала
JP4160868B2 (ja) * 2003-07-10 2008-10-08 日本たばこ産業株式会社 葉タバコ中のニトロソアミン含量の低減方法
KR20060128821A (ko) * 2003-08-19 2006-12-14 22엔디 센츄리 리미티드, 엘엘씨 감소된 노출 담배 제품
EP1851317B1 (en) 2005-02-23 2011-10-26 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
EP2087123B1 (en) 2006-10-13 2011-08-10 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
ES2716864T3 (es) 2007-06-06 2019-06-17 Monsanto Technology Llc Genes y usos para la mejora de plantas
BRPI0820042B1 (pt) 2007-11-12 2020-05-19 North Carolina State University método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana
EP2231861B1 (en) 2007-12-13 2014-10-29 Philip Morris Products S.A. Transgenic plants modified for reduced cadmium transport, derivative products, and related methods
WO2009091518A2 (en) * 2008-01-15 2009-07-23 Monsanto Technology, Llc Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
WO2011048009A1 (en) * 2009-10-22 2011-04-28 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
PH12012501393B1 (en) 2010-01-15 2017-10-25 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco
EP3447065A1 (en) 2010-09-03 2019-02-27 Philip Morris Products S.A. Heavy metal reduction in planta

Also Published As

Publication number Publication date
AP2015008563A0 (en) 2015-06-30
CN105247053B (zh) 2020-07-31
EP2935588A2 (en) 2015-10-28
US10563215B2 (en) 2020-02-18
US20150315603A1 (en) 2015-11-05
WO2014096283A2 (en) 2014-06-26
RU2015126909A (ru) 2017-01-27
PH12015501204B1 (en) 2022-07-15
JP2016508032A (ja) 2016-03-17
KR102253223B1 (ko) 2021-05-21
MX2015008084A (es) 2016-03-31
JP6693747B2 (ja) 2020-05-13
CN105247053A (zh) 2016-01-13
WO2014096283A3 (en) 2014-08-14
RU2735254C2 (ru) 2020-10-29
MX2019001598A (es) 2019-07-01
PH12015501204A1 (en) 2015-08-17
KR20150096451A (ko) 2015-08-24
CA2894955A1 (en) 2014-06-26

Similar Documents

Publication Publication Date Title
CA2894955C (en) Tobacco specific nitrosamine reduction in plants
US10415050B2 (en) Reduction of nicotine to nornicotine conversion in plants
US11685929B2 (en) Plants with shortened time to flowering
EP2773658B1 (en) Modulating beta-damascenone in plants
EP2751273B1 (en) Threonine synthase from nicotiana tabacum and methods and uses thereof
EP3169149B1 (en) Tobacco protease genes
US20170145431A1 (en) Modulation of nitrate content in plants
EP3346826A1 (en) Plants with reduced asparagine content
EP2586792A1 (en) Modulating beta-damascenone in plants
HK1196828A (en) Modulating beta-damascenone in plants
HK1196828B (en) Modulating beta-damascenone in plants
HK1194425A (en) Threonine synthase from nicotiana tabacum and methods and uses thereof
HK1194425B (en) Threonine synthase from nicotiana tabacum and methods and uses thereof

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126

EEER Examination request

Effective date: 20181126