RU2658431C1 - Dry nutritional formula for dietary nutrition - Google Patents

Abstract

FIELD: food industry; biotechnology.

SUBSTANCE: dry nutritional formula for dietary nutrition includes an enzymatic unfiltered hydrolysate in an amount of 67.8-69.8%, vegetable fat in an amount of 4.0-6.0%, milk powder in an amount of 3.5-4.5%, dried carrots in an amount of 5.0-6.0%, onions in an amount of 4.0-5.0%, salt in an amount of 3.0%, ground allspice in an amount of 0.04-0.07%, ground black pepper in an amount of 0.03-0.07%, riboflavin (dye) in an amount of 1.0-1.25%, pectin in an amount of 5.5-7.5% and 1-O-alkyl-glycerol esters in an amount of 1.0-3.0%. Components are expressed in wt%.

EFFECT: invention widens the range of dry nutritional formulas for dietary nutrition aimed at enhancing the immune status of the human body, intensifying the protective mechanisms of the human body and normalising lipid metabolism.

3 cl, 2 dwg, 6 ex

Description

The invention relates to the food industry, namely to the production of specialized products, and can be used in the manufacture of a nutrient mixture with high biological value and with pronounced therapeutic and prophylactic properties. The invention allows to obtain a dry nutrient mixture for introduction into basic and specialized diets for the correction of metabolic disorders in the human body.

Currently, various dry nutritional mixtures for dietary therapeutic and preventive nutrition have been developed, which include components of animal and vegetable origin, individual nutritional and biological active substances.

Known dry nutrient mixtures for functional purposes (RF Patent No. 2256381 "Method for the enrichment of dry nutrient mixtures", IPC A23L1 / 29, A23L1 / 10, A23L1 / 20, publ. 07/20/2005; RF Patent No. 2546274 "Dry nutrient mixture for functional purposes", IPC A23L1 / 29, A23L1 / 20, A23L1 / 212, publ. 04/10/2015), characterized by high biological value, the basis of which are chickpea flour and bran or lentil flour, as well as carrot powder, onion and cabbage, meal powder ginseng root, meal of rose hips and meal of nettle leaves.

Known dry nutritional mixtures include components that are not traditionally used simultaneously, which, apparently, will affect the sensory properties of the ready-to-eat product (not in dry form). Dry nutritional mixtures for dietetic nutrition include vegetable protein, protein hydrolyzate, water-soluble fat, carbohydrates, emulsifier, flavoring, dietary fiber, succinic acid, L-carnitine or its derivatives, selenium, vitamin and mineral complex (RF Patent No. 2306715 “Dry nutritional mixture for dietetic nutrition ", IPC A23J3 / 00, A23L1 / 30, published September 27, 2007; RF Patent No. 2348181" Dry nutrient mixture for dietetic nutrition (options) ", IPC A23L1 / 29, A23L1 / 305, A23J1 / 14, A23J1 / 20, published March 10, 2009). In addition to these components, the authors proposed to additionally include milk lactase-free protein, enzymes to improve the breakdown of nutrients in the gastrointestinal tract, probiotic and other substances.

In addition, the inclusion of vitamin-mineral premix, selenium on an organic carrier causes a significant increase in the cost of the proposed product.

A known composition of therapeutic and prophylactic action from marine mollusks containing an acid hydrolyzate from rapana meat and an alkaline hydrolyzate from mussel, in a ratio of 1:10, after evaporation under vacuum, the composition is balanced in amino acids. This composition is recommended for use in gerontological practice for the prevention of initial forms of atherosclerosis in elderly patients (Cl. RF 2541463, IPC A1K 35/56, A23L 1/333, A61P 9/10, publ. 02/10/2015).

Known biologically active food supplement from mussel meat, which is a hydrochloric acid hydrolyzate of raw materials containing organic food products of hydrolyzate and table salt. Moreover, the additive contains peptides with a molecular weight of from 300 to 700 D in an amount of 0.3-0.7 wt.%, Organic food products from 18 to 25 wt.% With a total solids content of 33 to 40 wt.%. As noted in the invention, the nutritional supplement increases the overall resistance of the body, stabilizes cellular and humoral immunity, accelerates the regeneration processes in damaged tissues and reduces the effects of toxic substances (Cl. RF 2284708, IPC A23L 1/30, A23L 1/333, A23J 1 / 04, publ. 10.10.2006).

Known biologically active food supplement that contains calcium alginate and protein hydrolyzate from mollusk meat in an amount, parts by weight: calcium alginate 210.5-280.0, protein hydrolyzate from mollusk meat 190-240.0. The product has multifunctional properties in the form most accessible to the body, as well as with increased biological activity (Cl. RF 2342857, IPC A23L 1/30, A61K 36/02, A23L 1/333, publ. 10.01.2009).

It is known to use a product based on a mussel meat hydrolyzate as a therapeutic and prophylactic drug in the diet. This preparation contains hydrochloric acid hydrolyzate of mussel meat with a melanoid content of more than 8 wt. % of the amount of solids and ascorbic acid in an amount of 0.25-0.6 wt. % As a result of the addition of ascorbic acid, the radioprotective activity of the product increases. The product is characterized by the following indicators: pH 5.0-5.6; solids content of 28-33 wt. %; the total nitrogen content of 1.7-2.5 wt. %; the content of amine nitrogen of 0.85-1.2 wt. % (RU 2043728, A23J 1/04, A23L 1/333, publ. 09/20/1995).

The disadvantage of all of the above analogues, developed on the basis of hydrolysates, is that they cannot be nutritious mixtures due to the fact that during the acid and alkaline hydrolysis of the protein substrate (especially long - 10-16 hours), almost complete destruction of amino acids is observed, especially tryptophan, tyrosine, cystine, cysteine, etc. [Chichibabin A.E. The main principles of organic chemistry. –M .: State Scientific and Chemical Institute of Chemical Liters, 1954. - T.1, p.33, 110], partial racemization and deamination of amino acids [p.703]. The destruction of the essential amino acid tryptophan and its absence in the substrate does not allow the synthesis of new proteins in the human body from other amino acids. In addition, when alkaline hydrolysis occurs, not only the decomposition of amino acids occurs, but also their isomerization and artefacts are possible [c. 110, 394], which is not harmless to the human body. In the process of acid and especially alkaline hydrolysis, some vitamins are inactivated or destroyed, including group B. [c.110]. All these analogues are dietary supplements for food, they are sources of only certain food substances, and not nutritious mixtures.

Closest to the proposed technical solution is dry protein condiments based on an enzymatic hydrolyzate from Scallop seaside and Sakhalin spizula (Likhacheva E.V. “Development of technology of hydrolysates and new protein products from Far Eastern bivalve mollusks”: Abstract of thesis for Candidate of Technical Sciences. - Vladivostok, 2010 .-- 24 p.). Protein seasonings include dried scallop hydrolysates and Sakhalin spizula (protein hydrolyzate), vegetable fat, milk powder, dried carrots, dried onions, table salt, ground allspice, ground black pepper, ground turmeric, riboflavin, sugar, starch and citric acid.

The disadvantage of the proposed dry nutritional mixtures is the additional introduction of digestible carbohydrates (sugar and starch). These carbohydrates are present in traditional foods in the diet. The additional introduction of digestible carbohydrates in human nutrition helps increase blood viscosity, which contradicts the principles of specialized dietetic nutrition.

The technical problem facing the invention is to expand the range of dry nutritional mixtures for dietetic nutrition, aimed at improving the immune status of the human body.

The technical result, which provides dietary therapeutic and prophylactic properties of the product, is achieved by the additional introduction of natural 1-O-alkyl-glycerol esters (AGE) and pectin into the dry nutrient mixture.

The technical problem posed is solved by the fact that in the known dry nutritional mixture for dietetic nutrition, including enzymatic unfiltered hydrolyzate, vegetable fat, dried milk, dried carrots, dried onions, table salt, ground allspice, ground black pepper and riboflavin, according to the invention in the composition of the mixture is additionally introduced 1-O-alkyl-glycerol esters and pectin, in the following ratio of components, wt.%:

enzymatic unfiltered hydrolyzate 67.8-69.8 vegetable fat 4.0-6.0 milk powder 3.5-4.5 pectin 5.5-7.5 dried carrots 5.0-6.0 dried onions 4.0-5.0 table salt 3.0 allspice 0.04-0.07 ground black pepper 0.03-0.07 riboflavin (dye) 1.0-1.25 1-O-alkyl-glycerol esters 1.0-3.0

To improve organoleptic characteristics (color, taste), turmeric in the amount of 0.03-0.07% or ground cinnamon in the amount of 0.05-0.06% is additionally introduced into the mixture.

The main component of the dry nutrient mixture is an enzymatic unfiltered hydrolyzate. As which, as in the prototype, it is proposed to use hydrolysates, in particular, from bivalves (Scallop seafood, Sakhalin spizula, Greenland cerripes, Stimson’s script) or cephalopods (Commander squid and Doflein octopus). Bivalves and cephalopods are sources of complete proteins, amino sugars, vitamins and minerals, as well as rare valuable compounds - taurine, carnosine, selenium, etc. In bivalves and cephalopods, the content and ratio of essential amino acids is close to the FAO / WHO ideal protein scale . Despite the low lipid content in the tissues of mollusks (no more than 2.5%), polyunsaturated fatty acids predominate in them. Hydrolysates of animal organs and tissues are represented by protein subunits and peptides, which are characterized by pronounced biological activity (Khavinson V.Kh., Kvetnaya TV “Regulatory peptides and homeostasis” // Russian Chemical Journal. - 2005. - T. XLIX. - No. 1 .-- S. 112-117). Their most important property is the ability to inhibit lipid peroxidation (Morozov VG, Khavinson V.Kh., Malinin VV “Peptide thymomimetics.” - St. Petersburg: Nauka, 2000. - 158 pp.), Which is of great importance in the implementation of antitumor protection, correction of violations of homeostasis and to slow down the aging process of the body. Enzymatic unfiltered hydrolysates contain the entire complex of nutrients present in the food tissues of bivalves and cephalopods. They have a creamy structure, a pleasant meaty, slightly sweet taste. Dry hydrolysates are able to quickly restore the original structure.

In the inventive mixture for dietary nutrition, the recommended amount of enzymatic unfiltered hydrolyzate (protein) is 67.8-69.8%, since a decrease from this level leads to a deterioration in the structural characteristics of the product, and an increase, despite the benefit of the protein, does not allow less valuable ingredients in the required quantities.

An additional introduction to the composition of the dry nutrient mixture of natural 1-O-alkyl-glycerol esters not only expands the range of dry nutrient mixtures, but also creates nutrient mixtures that positively affect the blood composition and the human immune system. Natural AGEs have pronounced biological activity, which is manifested in the inhibition of the utilization of atherogenic lipids, the development of hyperglycemia and membrane destruction of hepatocytes, the increase in the functional activity and the number of red and white blood cells (Kasyanov SP et al. “Pharmacological effects of a biologically active food supplement based on monoalkyl glycerols "// Pacific Medical Journal. - 2010. - No. 2. - P. 32-35; Latyshev N.A. et al." Alkyl-glycerol esters of marine organisms: distribution structure and biological activity vnost '// Izvestia TINRO. - 2012. - T. 169. - S. 261-277). It is known that AGE preparations have a positive effect on the immune (Yamamoto et al. "Activation of mouse macrophages by alkylglycerols, inflammation products of cancerous tissues" // Cancer Res. - 1988. - Vol. 48. - P. 6044–6049), regenerative (Maulik N. et al. "Miocardial salvage by 1-O-hexadecyl-sn-glycerol: possible role of peroxisomal dysfunction in ischemia reperfusion injury" // J. Cardiovascular Pharmacology. - 1994.-Vol. 24. - P. 486 -492), hematopoietic (Larbi SO et al. "The effects of alkoxyglycerols on haematopoiesis" // Arh. Int. Pharmacodyn. - 1968. - Vol. 173, N 1. - P. 71-86) and other systems.

An adequate level of consumption of alkyl glycerols for an adult is 1000.0 mg / day (“Unified sanitary and epidemiological and hygienic requirements for products subject to sanitary and epidemiological surveillance (control)”, Chapter II, Section 1. Safety and nutritional requirements of food products, Appendix 5 // Rospotrebnadzor. - 2010. - P. 398).

Natural AGE, used as a biologically active additive in the composition of a dry nutrient mixture, is obtained by technological hydrolysis of liver lipids of commander squid and hepatopancreas of king crab with the further application of a number of physicochemical methods (TU 9281-006-02698223-10, Certificate of State Registration of BAA "Lipidomarin" No. RU.77.99.11.003.E.008800.06.12 dated 05.06.2012).

The introduction of pectin in the composition of the nutrient mixture enriches the product with natural substances, since it is a source of natural dietary fiber, mono- and disaccharides, minerals and vitamin PP. The value of pectin for the human body is due to the ability to improve intestinal motility and peripheral blood circulation, reduce cholesterol. The enveloping and astringent properties of pectin favorably affect the condition of the mucous membrane of the gastrointestinal tract, which characterizes it as a light anti-inflammatory and analgesic natural remedy for peptic ulcers. Pectin has gelling properties and contributes to the creation of a viscous product structure.

The physiological need for dietary fiber, including in pectin, for an adult is 20 g / day, for children over 3 years old 10-20 g / day (Methodical recommendations MP 2.3.1.2432-08. "Norms of physiological requirements for energy and nutrients for various groups of the population of the Russian Federation", p. 16, 1st paragraph). Pectin is used as a structuring agent that provides dietary properties by reducing blood sugar and its sorption properties. It is advisable to maintain the pectin content in the dry nutrient mixture in the range of 5.5-7.5%.

The additional introduction of ground cinnamon into the composition of the dry mix for dietetic nutrition contributes to the creation of a flavoring composition of the target product, along with other flavors: table salt, ground turmeric, ground allspice and black pepper. When eaten, it increases the secretion of gastric juice and improves the digestive process. The composition of cinnamon includes natural polyphenols, terpenes, terpenoids and other substances that have a beneficial effect on the human body, as well as antibacterial, antiviral and antifungal properties. Achieving the claimed technical result is also ensured by the introduction of ground cinnamon in an amount of 0.05-0.06%. An increase in the concentration of ground cinnamon in the composition of the nutrient mixture more than 0.06% leads to an increase in cinnamon odor, which is undesirable for a dietary product.

Turmeric has a lot of healing properties: it normalizes metabolism, has a healing effect, has antibacterial properties, and improves blood circulation.

The coloring substance curcumin, contained in turmeric, favorably affects the state of the gallbladder. Essential oil activates the liver. Also, this plant is used to eliminate unpleasant sensations of irritation and burning, the treatment of dermatitis and allergies. However, turmeric belongs to potent spices and in order to avoid negative consequences and achieve the claimed technical result, as well as to give the mixture a pleasant yellowish tint, the applicant recommended introducing ground turmeric into the composition of the dry mixture in an amount of 0.03-0.07%.

The inclusion in the mixture of food additives (vegetable fat, milk powder, dried carrots, dried onions) at the stated intervals increases the nutritional value of the mixture and helps to create good organoleptic properties of the target dietary product.

Riboflavin (vitamin B ₂ ) is a needle-shaped crystals of yellow-orange color, as a result of which the introduction of riboflavin in an amount of 1.0-1.25% gives the inventive dry mixture a pleasant light yellow color. In addition, it plays a key role in maintaining human health. Like the rest of B vitamins, riboflavin supports energy production, and also takes an active part in the metabolism of fats, carbohydrates and proteins. Vitamin B _{2 is} indispensable in the formation of red blood cells and helps to saturate the blood with oxygen during breathing. In addition, vitamin B ₂ stimulates the production of antibodies, and is also necessary for regulating the growth of the human body and reproductive function. In addition, this vitamin is of great importance for the health of the skin, nails, hair growth and overall good health, including participating in the regulation of the thyroid gland. It also helps in the prevention or treatment of many types of eye diseases, including some types of cataracts.

2^оС в течение 12 мес. To prepare a dry nutrient mixture for dietary nutrition, take the components within the declared intervals, mix thoroughly and pack 30 g each in packages of polymer film materials, hermetically sealed on a vacuum capping machine with a residual pressure in the working volume of the chamber of 0.0107-0120 MPa (80 -90 mm Hg) and stored at a temperature not exceeding 20

2 ^о С for 12 months.

For dietary nutrition aimed at increasing the immune status of the human body, it is recommended to take a dry nutrient mixture containing 300-1200 mg of AGE for daily intake.

The summary table 2 for blood shows the resulting indicators of their successful use (examples 1-6). The lower limit is 300 mg, which is justified by examples, and the upper limit is proposed by applicants to 1200 mg, as Rospotrebnadzor recommends limiting to 1000 mg per day for an adequate dose (there is a link in the text). Given that the mixtures are preventive and therapeutic in nature, the dose was slightly increased from an adequate daily intake.

The claimed invention is illustrated by the following examples and tables.

Table 1 shows the component composition of dry nutrient mixtures and the content of 1-O-alkyl-glycerol esters. Table 2 presents the indicators of lipid metabolism and a clinical blood test in patients taking a dry nutrient mixture.

Example 1

To obtain a dry nutrient mixture based on a dry enzymatic unfiltered hydrolyzate from a bivalve mollusk, Stimson's scripts use dry components in the ratios indicated in the table. one.

2^оС в течение 12 мес. Порция сухой питательной смеси содержит 300,0 мг препарата АГЭ.A mixture of these components is thoroughly mixed, packaged in 30 g packages of polymer film materials, hermetically sealed on a vacuum seal machine and stored at a temperature not exceeding 20

2 ^о С for 12 months. A portion of the dry nutrient mixture contains 300.0 mg of the AGE preparation.

The dry nutrient mixture obtained in example 1 was used in the diet of patients with lipid metabolism disorders (group of 8 people) of the age group of 50-60 years daily for 60 days in one packet of the mixture. Before and after the experiment, patients underwent a clinical blood test and determined lipid metabolism. It was found (Table 2) that in all patients the intake of a nutrient mixture with AGE was accompanied by normalization of lipid metabolism: there was a tendency to a decrease in blood triglycerides and total cholesterol, an increase in the proportion of cholesterol in high-density lipoproteins and a decrease in its content in low and low lipoproteins very low density.

In addition, the stimulating effect of AGE on erythropoiesis and hemoglobin synthesis was noted. The decrease in ESR and the number of leukocytes in the blood is associated with an increase in the immune status and resistance of the patient's body.

At the end of the intake of the nutrient mixture with AGE, patients noted an improvement in sleep, overall well-being and increased performance.

The data obtained should be considered as an intensification of the protective mechanisms of the patient’s body, caused by the action of the natural components of the nutrient mixture, including alkyl glycerol esters.

Example 2

To obtain a dry nutrient mixture based on a dry enzymatic unfiltered hydrolyzate from bivalve scallop of the scallop, dry components are used in the ratios indicated in the table. 1. The preparation of the mixture, its capping and other procedures are carried out, as in example 1. In a sample of dry nutrient mixture weighing 30 g contains 600.0 mg of the drug AGE.

The dry nutrient mixture obtained in example 2 was used in the diet of patients (group of 10 people) in the age group of 50-65 years daily for 45 days in one packet of the mixture. Before and after the experiment, patients underwent a clinical blood test and determined lipid metabolism. In all patients, the intake of a nutrient mixture with AGE, as in example 1, noted a pattern of changes in lipid metabolism and a general blood count, an improvement in overall well-being and normalization of sleep.

In this group, tumor markers (CEA and PSA in men) were additionally identified. Before administration, the CEA indicator (norm 0-5.0 ng / ml) was 1.2-4.0 ng / ml, after administration - 0.2-0.96 ng / ml. The PSA indicator (norm 0-7.0 IU / ml) also decreased: before administration, it was in the range of 0.32-1.6 IU / ml, after administration it was 0.22-0.8.

Example 3

To obtain a dry nutrient mixture based on a dry enzymatic unfiltered hydrolyzate from a bivalve mollusk, Sakhalin spizula use dry components in the ratios indicated in the table. 1. In a sample of a dry nutrient mixture weighing 30 g, the content of AGE is 1200.0 mg.

The dry nutrient mixture obtained in example 3 was used in the diet of patients (group of 6 people) of the age group 55-65 years daily for 30 days in one packet of the mixture. Before and after the experiment, patients underwent a clinical blood test and determined lipid metabolism.

The pattern of changes in the general well-being of patients as a result of taking a nutrient mixture with AGE, lipid metabolism and a general blood test, as in example 1, changes in tumor markers, as in example 2.

Example 4

To obtain a dry nutrient mixture based on a dry hydrolyzate from bivalve mollusk of Greenland creep, dry components are used in the ratios indicated in the table. one.

In a sample of a dry nutrient mixture weighing 30 g, the AGE content is 450.0 mg.

The dry nutrient mixture obtained in example 4 was used in the diet of patients on an outpatient basis (group of 8 people) daily for 45 days in one packet of the mixture. Before and after the experiment, patients underwent a clinical blood test and determined lipid metabolism.

The pattern of changes in the parameters of the general analysis of blood, lipid metabolism, as well as the general well-being of patients as a result of taking the nutrient mixture with AGE in example 1.

Example 5

To obtain a dry nutrient mixture based on a dry enzymatic unfiltered hydrolyzate from a cephalopod mollusk Commander squid, dry components are used in the ratios indicated in the table. one

In a sample of a dry nutrient mixture weighing 30 g, the content of AGE is 750.0 mg.

The dry nutrient mixture obtained in Example 5 was used in the diet of university employees (teachers) (group of 10 people) every day for 30 days in one packet of the mixture. Before and after the experiment, patients underwent a clinical blood test and determined lipid metabolism.

The pattern of changes in the parameters of the general analysis of blood, lipid metabolism, as well as the general well-being of patients as a result of taking the nutrient mixture with AGE in example 1.

The pattern of changes in the general well-being of patients as a result of taking a nutrient mixture with AGE, lipid metabolism and a general blood test, as in example 1, changes in tumor markers, as in example 2.

Example 6

To obtain a dry nutrient mixture based on a dry enzymatic unfiltered hydrolyzate from the cephalopod mollusk of the octopus Doflen, dry components are used in the ratios indicated in the table. one

In a sample of a dry nutrient mixture weighing 30 g, the AGE content is 450.0 mg.

The dry nutrient mixture obtained in Example 6 was used in the diet of university staff (teachers) (group of 10 people) every day for 60 days in one packet of the mixture. Before and after the experiment, patients underwent a clinical blood test and determined lipid metabolism and tumor markers.

The pattern of changes in the parameters of the general analysis of blood, lipid metabolism, as well as the general well-being of patients as a result of taking the nutrient mixture with AGE in example 1.

The pattern of changes in the general well-being of patients as a result of taking a nutrient mixture with AGE, lipid metabolism and a general blood test, as in example 1, changes in tumor markers, as in example 2.

Thus, it has been experimentally proved that the use of the developed nutrient mixtures contributes to the intensification of the protective mechanisms of the human body and the normalization of lipid metabolism.

Claims (4)

1. Dry nutritional mixture for dietetic nutrition, including enzymatic unfiltered hydrolyzate, vegetable fat, milk powder, dried carrots, dried onions, common salt, allspice, ground black pepper and riboflavin, characterized in that it additionally contains pectin and 1 -O-alkyl-glycerol esters, in the following ratio, wt.%: enzymatic unfiltered hydrolyzate 67.8-69.8 vegetable fat 4.0-6.0 milk powder 3.5-4.5 pectin 5.5-7.5 dried carrots 5.0-6.0 dried onions 4.0-5.0 table salt 3.0 allspice 0.04-0.07 ground black pepper 0.03-0.07 riboflavin (dye) 1.0-1.25 1-O-alkyl-glycerol esters 1.0-3.0 2. The dry nutritional mixture according to claim 1, characterized in that it further contains ground turmeric in an amount of 0.03-0.07% or ground cinnamon in an amount of 0.05-0.06%. 3. The dry nutrient mixture according to claim 1, characterized in that the daily intake of 1-O-alkyl-glycerol esters in the composition of the nutrient mixture is 300.0-1200.0 mg.

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