JP2002255846A - Oral composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an oral composition excellent in immunoactivating effect and infectious respiratory disease prophylactic effect as well as nutritive/tonic effect and recovery-from-fatigue effect. SOLUTION: This oral composition comprises at least one component selected from lactoprotein hydrolyzates, collagen protein hydrolyzates, soybean protein and its hydrolyzate and substantially no lipid.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention improves immunity by improving various physical conditions such as deterioration of sustained physical strength, chronic fatigue, muscle pain, stiff shoulders, and fatigue after waking up. The present invention relates to an oral composition having a function of improving power loss and preventing the occurrence of infectious respiratory disease. The composition of the present invention is not limited to a subject, but is particularly useful for a person with a frail constitution; a person with a remarkably biased daily intake of nutrients; a middle / elderly person; hospitalization, bedridden state, and often staying home. It is intended for people who have restrictions on their behavior.

[0002]

2. Description of the Related Art In Japan at present, the number of people with reduced physical strength and immunity is increasing, causing various problems. In particular, the number of people who complain of discomfort in daily life, such as chronic fatigue and stiff shoulders, is increasing, especially in the young age group, and the incidence of infectious diseases such as cold is directly or indirectly involved in the cause of death. The number of elderly people is increasing in the elderly
One is becoming an issue in the welfare administration. (Fundamental Survey on National Life 1997) The cause of discomfort in daily life is not known, but it is known that constant stress exposure adversely affects the autonomic nervous system and the whole body immune system. Regular stress varies greatly depending on the environment in which the individual is located, but in general cases, physical stress resulting from extreme lack of exercise due to hospitalization or withdrawal from facilities / homes; Autonomic nervous system and skin caused by low temperature and dryness caused by prolonged stay in well-equipped homes, offices and public places
Stress on mucosal tissues; stress on the optic nervous system and upper body muscles caused by prolonged staring at specific parts such as television, video, OA equipment, the Internet, and computer games; respiration by air pollutants such as nitric oxide compounds and suspended particles Stress on the organs; stress due to constant sleep deprivation due to disturbance in lifestyle and working conditions; increased frequency of use of processed / semi-processed foods and qualitative and quantitative disturbances in eating habits Metabolic stress due to extreme over-balance of the intake nutrient balance. These factors are often diversified, and the means of alleviating their stress are often virtually impossible, so they are often addressed with nutritional drinks and vitamins. However, these means cannot sufficiently solve the above-mentioned problems, so that prompt proposal of a countermeasure is desired.

[0003]

Under such circumstances, vitamin B group and Chinese herbal plants are generally replenished, but there are many cases in which the effect cannot be obtained, and the problem has not been solved. Recently, a solution by ingesting a specific food material has been proposed. For example, cruciferous plants such as garlic, cabbage, perilla, and onion; fungi such as agarisk, shiitake, meshimakobu, and maitake; and propolis. However, these materials
There was a problem that the effect could not be solved as a result, because a sufficient effect could not be obtained, or it was very expensive and could not be taken for a long time. Further, even if a food containing protein is ingested in a meal, the effect of ingesting the protein cannot be obtained because the intake amount is insufficient or the lipid content is ingested together with the protein.

[0004]

Means for Solving the Problems The present inventors have conducted intensive studies on inexpensive natural food materials which can be drunk for a long time, and as a result, surprisingly, milk protein, soy protein, collagen protein and their proteins have been surprisingly studied. The inventor has found that ingestion of the hydrolyzate provides not only a nutrient tonic effect and a fatigue recovery effect, but also an immunostimulatory effect and an infectious respiratory disease preventive effect, thereby completing the present invention.

Item 1 An oral composition characterized by containing at least one selected from milk protein hydrolyzate, collagen protein hydrolyzate, soy protein and its hydrolyzate, and substantially containing no lipid. Item 2. Milk protein hydrolyzate, collagen protein hydrolyzate, soy protein and its hydrolyzate have an average molecular weight of 2
The oral composition according to claim 1, wherein the oral composition is from 00 to 10,000. Item 3. The oral composition according to any one of Claims 1 and 2, further comprising a crude drug and vitamins. Item 4. The composition according to any one of claims 1 to 3, wherein the oral composition is a liquid having a pH of 6.5 or less. [Detailed description of the invention]

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The milk protein hydrolyzate used in the present invention is a hydrolyzate of casein and whey protein. Casein is a type of phosphoprotein and is classified into α (α _S and κ), β, and γ. Examples of whey proteins include α-lactalbumin, β-lactalbumin, lactoferrin and the like.

The soybean protein and its hydrolyzate used in the present invention are not particularly limited as long as they are proteins derived from soybean, but those produced from defatted soybean are preferred.
Among them, it is most preferable to use a protein obtained by separating from soymilk made from defatted soybean because it has a low lipid content.

The collagen protein hydrolyzate used in the present invention is not particularly limited.
In addition to mammals such as pigs, sheep and goats, birds such as chickens and turkeys, and teleost fish such as tuna, skipjack, saury, sardines, herring, cod, trout, anglerfish, flounder, eel,
Cartilage fish such as sharks and rays, crustaceans such as crabs and shrimp,
Hydrolysates of collagen proteins obtained from sea pods (squirts) and sea rats (sea cucumber) are preferred. Among them, cows, pigs,
In addition to chicken skin tissue, cartilage tissue, bony tissue, vascular tissue, hydrolysates of collagen proteins obtained from teleost fish, cartilage fish, and crustacean tissue, more preferably chicken skin tissue and cartilage tissue, Hydrolysates of collagen proteins obtained from tissues of teleost fish, cartilage fish, and crustacea, in addition to bone tissue and vascular tissue, are most preferable, and the type I, type II, and type III collagen protein hydrolysates are preferably used. Decomposed products can be used.

As a method for hydrolyzing a protein, an acid, an alkali or an enzyme is generally used, and it may be carried out simply by heating. Although any of these methods may be used, hydrolysis using an enzyme can limit the hydrolysis point to some extent by utilizing the substrate specificity of the enzyme. Is preferable because the value of becomes substantially constant. Furthermore, these hydrolysates are subjected to processes such as separation, fractionation, purification, concentration, and drying as necessary, and are easily processed into liquids, powders, and the like to be easily incorporated into various dosage forms, The initial effect can be enhanced by processing the molecular weight distribution of the polypeptide constituting the hydrolyzate for each application or by processing it into a single polypeptide (including oligopeptides such as dipeptides).

[0010] As the protein hydrolyzate of the present invention, those having an average molecular weight of 200 to 1800000 are usually used. Among them, 200 to 10,000 are preferable, and those having an average molecular weight of 200 to 5,000 are preferable from the viewpoint of palatability and digestibility. In addition, when a liquid dosage form is used, 6
A value of from 00 to 3500 is the most preferable in terms of prescription technology.
In the composition of the present invention, even if a protein component having a molecular weight outside the above range is mixed, there is no problem as long as the effect is obtained. As a method for measuring the molecular weight, a gel filtration method generally used for measuring the molecular weight of a protein or polypeptide can be used. The type of resin used at this time is appropriately selected depending on the distribution state of the molecular weight to be measured. For example, using high performance liquid chromatography,
× 100 cm column, pH 5.0 phosphate buffer, 40 ° C,
It is performed under the conditions of a flow rate of 30 ml / hr and 220 nm (ultraviolet ray detector). The protein is not particularly limited as long as it is a milk protein hydrolyzate, a collagen protein hydrolysate, a soy protein and a hydrolyzate thereof, but a collagen protein hydrolyzate, a soy protein and a hydrolyzate thereof are preferable. Most preferred are collagen protein hydrolysates and soy protein hydrolysates. The amount is not particularly limited, but the daily intake is usually 1 g or more. Among them, about 5 to 15 g is preferable. If the amount is less than 1 g, the desired effect may not be expected due to the nutritional state at the time of ingestion. Is not preferred because it cannot be obtained.

[0011] The compositions of the present invention are substantially free of lipids. Specifically, the content is a content that can be displayed as zero when the composition is lipid-displayed based on the nutrition improvement method, that is,
It shall be less than 0.5 g per 100 g or 100 ml of the composition (Nutrition Labeling Standard Article 3, Paragraph 1, Item 6, Provision, Appendix 1). When the composition contains the above lipids,
It is not preferable because a sufficient effect may not be obtained.

The composition of the present invention preferably further contains a crude drug and vitamins. The herbal medicines used in the present invention are not particularly limited, but preferred are Asenyaku, Fenka, Eleuthero, Forage, Yellow sperm, Licorice,
What birds, kakkon, persimmon leaves, almond jelly, tamatake, cinnamon bark, gokahi, sanshuyu, saffron, yamako, sanyaku, peony, shrinking sand, ginger, river
、, Saiyama sashimi, choji, 皮 、, Toki, Tochu, capsicum, ginseng, garlic, Tenmon winter, Bakumon winter, 苡 jin, lotus meat. Among them, Asenyaku, Fenka, Elephantwood, Yellow Sperm, Licorice, Persimmon leaf, Gokakin, Saffron, Yamashiko, Yamayaku, Shakuyaku, Shrinked Sand, Ginger, Seiyo Yamashiko, Toji, Chen, Toki, Tochu, Tonaka, Capsicum, Ginseng, garlic, and peony are preferred, especially Asenyaku, eleuthero, persimmon leaves, yellow sperm, pentacafe, saffron, yamashiko, peony, ginger, western yamashiko, toki, tounaka, capsicum, ginseng, garlic, garlic, and kaijin. Is preferred.

These herbs are naturally dried, hot air dried,
Freeze-dried or fermented products may be used as they are, or those obtained by performing processes such as concentration, extraction, and powdering according to a conventional method. Examples of such a method include a method described in JP-A-61-171427. The amount is not particularly limited, but may be about 1 mg to 3 g per day in terms of solid content as a daily intake. 2 mg to 5
About 00 mg is preferable. If less than 1 mg,
If the desired effect cannot be expected, and if the amount exceeds 3 g, the effect of adding the compound cannot be obtained, which is not preferable for economic reasons.

The vitamins used in the present invention are not particularly limited, but specifically, vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , vitamin C,
Vitamin D, vitamin E, vitamin K, niacin, pantothenic acids, folic acid, retinoids, biotin, carotenoids, tocophenols, essential unsaturated fatty acids, ubiquinone, cholines.

[0015] The vitamin B ₁ is, including thiamine and its derivatives. Representative examples include thiamine, thiamine hydrochloride, thiamine nitrate, thiamine monophosphate, thiamine diphosphate, thiamine triphosphate, octiamine, sicotiamine, thiamine disulfide,
Thiamine dicetyl sulfate, bisthiamine nitrate,
Disetiamine hydrochloride, fursultiamine hydrochloride, alitiamine, bisibutiamine, bisbenthamine, fursultiamine, prosultiamine, benfotiamine.

The amount is not particularly limited.
About 0.2 mg to 100 mg per day can be added. Of these, about 1 to 25 mg is preferable. 0.2
If the amount is less than 1 mg, the expected effect cannot be expected and 1
If the amount is more than 00 mg, the effect of the compounding cannot be obtained, so that it is not preferable for economic reasons.

Vitamin B ₂ is a general term for flavin mononucleotide, flavin adenine nucleotide, and derivatives thereof. Representative examples include riboflavin, flavin mononucleotide, flavin adenyl nucleotide, flavin adenyl nucleotide sodium,
And riboflavin sodium phosphate and riboflavin butyrate. Of these, riboflavin, flavin adenyl nucleotide sodium, riboflavin sodium phosphate, and riboflavin butyrate are preferred.

The amount is not particularly limited.
As a daily intake, about 0.2 to 50 mg can be added. Of these, about 1 to 15 mg is preferable. 0.2m
If less than g, the expected effect cannot be expected and 50
If the amount is more than mg, it is not preferable from the economical point of view because the effect of the compounding cannot be obtained.

Vitamin B ₆ includes pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5'-phosphate,
Includes pyridoxal 5'-phosphate, pyridoxamine 5'-phosphate, and derivatives thereof. Representative examples include, in addition to the above six compounds, pyridoxine hydrochloride,
Pyridoxal phosphate, 4-pyridoxic acid, pyridoxine 5′-β-glucoside. Of these, pyridoxine hydrochloride and pyridoxal phosphate are preferred.

The blending amount is not particularly limited.
About 0.1 mg to 100 mg can be added as a daily intake. Of these, about 1 to 10 mg is preferable. 0.1
If the amount is less than 1 mg, the expected effect cannot be expected and 1
If the amount is more than 00 mg, the effect of the compounding cannot be obtained, so that it is not preferable for economic reasons.

[0021] shows the corrinoids the vitamin B ₁₂ class. Representative examples include adenosylcobalamin, methylcobalamin, hydroxocobalamin, aquacobalamin, cyanocobalamin, hydroxocobalamin hydrochloride, hydroxocobalamin acetate. Of these, hydroxocobalamin, cyanocobalamin, hydroxocobalamin hydrochloride, and hydroxocobalamin acetate are preferred.

The blending amount is not particularly limited.
About 0.2 μg to 200 μg can be added as a daily intake. Of these, about 1 μg to 60 μg is preferable. 0.2
If the amount is less than μg, the desired effect cannot be expected and 2
If the amount is more than 00 μg, the effect of the compounding cannot be obtained, so that it is not preferable for economic reasons.

Representative examples of vitamin Cs include ascorbic acid, monodehydroascorbic acid, dehydroascorbic acid, erythorbic acid, ascorbic acid-2-
Phosphoric acid, ascorbic acid-2-glucoside and salts thereof. Of these, ascorbic acid, sodium ascorbate, calcium ascorbate, erythorbic acid, ascorbic acid-2-phosphate, and ascorbic acid-2-glucoside are preferred.

The blending amount is not particularly limited.
About 10 mg to 5000 mg can be blended daily. Of these, about 50 to 1000 mg is preferred. If the amount is less than 10 mg, the desired effect cannot be expected. If the amount exceeds 5000 mg, the effect of the combination cannot be obtained, and diarrhea may be caused in some people, which is not preferable.

[0025] Vitamin D is a general term for a factor for preventing wilt disease which is produced when provitamin D having a 5,7-diensterol skeleton is subjected to ultraviolet treatment. Representative examples include vitamin D ₂ , vitamin D ₃ , vitamin D ₄ , vitamin D ₅ , vitamin D ₆ , and vitamin D ₇ . Of these, vitamin D ₂ (ergocalciferol) and vitamin D ₃ (cholecalciferol) are preferred.

The blending amount is not particularly limited.
About 10 to 2000 international units can be blended per day. Of these, about 50 to 200 international units are preferred.
If it is less than 10 international units, the expected effect cannot be expected. If it exceeds 200 international units, long-term ingestion may cause abnormal increase in serum calcium concentration and cause renal failure and arteriosclerosis. It is not preferable because the property comes out.

Vitamin K is a derivative of naphthoquinone and includes vitamin K ₁ (phyloquinone), vitamin K ₂ (menaquinone), menadione and their reduced form (hydroquinone type). The amount is not particularly limited, but may be 1 μg to 3000 mg per day.
About 0 μg can be added. 50 μg to 5000 μ of these
g is preferable. If the amount is less than 1 μg, the desired effect cannot be expected, and if it exceeds 3000 μg, there is a possibility of causing ischemic heart disease, which is not preferable.

Niacin is a generic term for pyridine-3-carboxylic acid derivatives. Representative examples include nicotinic acid, nicotinamide, nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, reduced nicotinamide adenine dinucleotide, reduced nicotinamide adenine dinucleotide phosphate, pyridine-3- Methanol, pyridine-3-aldehyde,
N'-methylnicotinamide. Of these, nicotinic acid, nicotinamide and pyridine-3-methanol are preferred.

The blending amount is not particularly limited.
About 2 mg to 500 mg can be added as a daily intake. Of these, about 12-60 mg is preferred. 2m
If less than g, the expected effect cannot be expected and 50
If it exceeds 0 mg, it is not preferable because discomfort may occur.

The pantothenic acids include pantothenic acid and its derivatives. Representative examples include pantothenic acid and its salts, pantethein, phosphopantothein-S-sulfonic acid, 4′-O- (β-D-glucopyranosyl) D-pantothenic acid, panthenol.
Of these, calcium pantothenate, sodium pantothenate and pantothenol are preferred.

The blending amount is not particularly limited.
About 1 mg to 1000 mg can be added as a daily intake. Of these, about 5 to 50 mg is preferable. 1mg
If less than 100, the expected effect cannot be expected and 100
If the amount is more than 0 mg, the effect of the compounding cannot be obtained, so that it is not preferable for economic reasons.

Folic acid is a general term for pteroylglutamic acid and its derivatives. Representative examples include pteroylglutamic acid, pteroylpolyγ-glutamic acid derivatives, and leucovorin. Although the amount is not particularly limited, the daily intake is 10 μg to 10 μg.
About 00 μg can be blended. 100 μg to 200
About μg is preferable. If the amount is less than 10 μg, the desired effect cannot be expected, and if it exceeds 1000 μg, unpleasant symptoms may be caused, which is not preferable.

The term "retinoid" is a generic term including a vitamin A1 system having retinoic acid as a basic structure, a vitamin A2 system having 3-dehydroretinol as a basic structure, and related substances. Representative examples include retinol, retinal, retinoic acid, 3-dehydroretinol, 3-dehydroretinal, 3-dehydroretinoic acid, anhydroretinol, retroretinol, isoanhydroretinol, and their esterified products (eg, acetic acid). Retinol, retinol palmitate). Of these, esterified compounds are preferred.

The blending amount is not particularly limited.
About 50 to 5000 international units can be blended per day. Of these, about 500 to 2000 international units are preferred. If the amount is less than 50 international units, the intended effect cannot be expected. If it exceeds 5000 international units, short-term or long-term ingestion may cause various acute or chronic poisoning symptoms. Not preferred.

Representative examples of biotin include biotin, biocytin, oxybiotin, biotin sulfoxide, and biotin sulfone. Although the amount is not particularly limited, the daily intake is 1 μg to 1 μg.
About 000 μg can be blended. 10 μg to 500 of these
About μg is preferable. If the amount is less than 1 μg, the desired effect cannot be expected, and if it exceeds 1000 μg, the effect of the compounding cannot be obtained, which is not preferable for economic reasons.

The carotenoid is a general term for a substance having a basic skeleton of a tetraterpenoid having 40 carbon atoms having 8 isoprene skeletons having 5 carbon atoms. Representative examples include α-carotene, β-carotene, γ-carotene, lycopene, lutein, zeaxanthin, canthaxanthin,
Astaxanthin. Among them, β-carotene, lycopene, lutein, and zeaxanthin are preferred.

The blending amount is not particularly limited.
A daily intake of about 0.5 mg to 100 mg can be added. Of these, about 1 to 10 mg is preferable. 0.5
If the amount is less than 1 mg, the expected effect cannot be expected and 1
If the amount is more than 00 mg, the effect of the compounding cannot be obtained, so that it is not preferable for economic reasons.

The tocophenols include vitamin E and its esterified products. Vitamin E is α-tocopherol, β-tocopherol, γ-tocopherol,
It is a general term for σ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol, and σ-tocotrienol, and examples of esterified products include succinates and acetates. this house,
α-tocopherol, succinic acid-α-tocopherol,
Succinic acid-α-tocopherol calcium, acetic acid-α-
Tocopherol is preferred.

The blending amount is not particularly limited.
Daily intake of α-tocopherol, 1mg ~
About 600 mg can be blended. 5mg-100m
g is preferable. If the amount is less than 1 mg, the intended effect cannot be expected. If the amount is more than 600 mg, discomfort may occur, which is not preferable.

The essential unsaturated fatty acids include linoleic acid, α-linolenic acid and derivatives thereof. The amount is not particularly limited, but as a daily intake,
About 1 mg to 5000 mg can be added. 50m of which
It is preferably about g to 200 mg. If the amount is less than 1 mg, the desired effect cannot be expected, and if it exceeds 5000 mg, the effect of the compounding cannot be obtained, which is not preferable for economic reasons.

Ubiquinone is 2,3-dimethoxy-5
It is a generic term for compounds having a methyl-1,4-benzoquinone structure as a mother nucleus and having an isoprenoid side chain at the 6-position, and is also called coenzyme Q. Representative examples include ubiquinone, ubiquinol, ubichromenol, ubichromanol, ubisemiquinone, fumigatine, plastquinone A,
Rhodoquinone, idepenone, piericidin A, uranium thiogliocladin and the like.

The blending amount is not particularly limited.
About 1 mg to 100 mg can be added as a daily intake. Of these, about 10 to 60 mg is preferable. 1m
If less than g, the expected effect cannot be expected and 10
If the amount is more than 0 mg, the effect of the compounding cannot be obtained, so that it is not preferable for economic reasons.

The cholines include choline, phosphatidylcholine, and sphingomyelin. Of these, phosphatidylcholine is preferred. Although the amount is not particularly limited, the daily intake is 10 mg to 5000 m.
g can be blended. 100mg to 2000mg
The degree is preferred. If the amount is less than 10 mg, the desired effect cannot be expected, and if it exceeds 5000 mg, the effect of the compounding cannot be obtained, which is not preferable for economic reasons.

The composition of the present invention can be prepared in various forms such as, for example, lumps, liquids, syrups, powders, and jellies in accordance with a conventional method. Drinks (drinks) such as water, juice and tea; powdered drinks such as powdered juice and powdered soup;
Cookies, biscuits, chewable tablets, chewing gum, candy, gummy, wafers, rice crackers and other confectionery; dressings, sauces, seasonings such as powdered seasonings; solid nutritional supplements, powdered nutritional supplements, porridge, cereals, soups, breads , Noodles, rice cakes and other food products; functional foods such as foods for the sick, foods for specified health use, and dietary supplements;
Quasi-drugs and the like. Among the above-mentioned forms, a liquid, a paste, a jelly, and a powder are preferable, and the liquid is most preferable.

When the composition of the present invention is in a liquid state, the pH of the composition is preferably 6.5 or less from the viewpoint of the stability over time of the composition, and most preferably 5.5 or less. preferable.

In the composition of the present invention, if necessary, as long as the effects of the present invention are not impaired, components usually used in medicines and foods, for example, other medicinal components, micronutrients (minerals, polyphenols, etc.) ), Anionic surfactants, nonionic surfactants, cationic surfactants, amphoteric surfactants, alcohols (lower alcohols, polyhydric alcohols and their ester derivatives, etc.), organic and inorganic acids and their salts, inorganic salts Water-soluble polymers, excipients, extenders, sweeteners, flavors, colorants, sours, flavors, antioxidants, seasonings, dietary fiber, preservatives, and other known food ingredients; , Alcoholic beverages, fruits, vegetables, root crops, mushrooms, potatoes, bifidobacteria, lactic acid bacteria, algae, yeast, green tea, black tea, cereal germ, beans other than soybeans, legume germ, seeds, Kairui, wild grass, native form, etc., or juice, puree, dried product, extract,
It can be blended in the form of dry powder, extract powder, crushed material, and the like.

The composition of the present invention can be taken once or several times a day. Milk protein hydrolyzate, collagen protein hydrolysate, a composition containing at least one component selected from soybean protein and its hydrolysate, and containing substantially no lipids, is a nutrient tonic,
It can be applied for the purpose of recovery from fatigue, immunostimulation, and prevention of infectious respiratory diseases. In particular, among them, it is preferable to use them for the purpose of recovery from fatigue and immunostimulation. Specific examples of infectious respiratory diseases include common cold, epidemic cold, acute bronchitis, acute bronchitis, chronic bronchitis, pneumonia, pulmonary opportunistic infection and the like.

These oral compositions are quasi-drugs for nutritional tonicity, recovery from fatigue and nutritional supplements, health foods for immunostimulation and nutritional tonicity, prevention of infectious respiratory diseases, nutritional supplementation, etc. , Food for the elderly, food for the sick, and the like.

[0049]

EXAMPLES Hereinafter, the present invention will be described in more detail based on examples and experimental examples, but the present invention is not limited to these examples. Unless otherwise specified, [%] indicates [% by weight].

EXPERIMENTAL EXAMPLE 1 Fatigue Recovery Effect A protein component was prepared by crushing bovine bone containing cartilage, defatting and removing minerals, and then hydrolyzing with a protease to obtain an average molecular weight of about 3500. Hydrolyzate derived from collagen protein (trade name: GELITA-SOL)
D2, manufactured by DGF Co.) and collagen obtained from cow hide having an average molecular weight of about 3000 using protein hydrolase.
A hydrolyzate derived from a collagen protein (trade name: GELITA-SOL D1, manufactured by DGF) was used. In the animal experiment, 18 8-week-old male SD rats were subjected to the following 3
Performed by dividing into groups. That is, 0.02% N
A control diet group in which G-nitro-L-arginine and 5% casein were added to the standard experimental diet, a bone collagen-derived hydrolyzate group in which 5% bone collagen-derived hydrolyzate was added in place of casein in the control diet, and a control diet The skin collagen-derived hydrolyzate group was prepared by adding 5% skin collagen-derived hydrolyzate instead of casein, and administration was free. They were bred for 5 weeks and their motility using a treadmill was measured. The motility was measured by placing the rat in a treadmill fixed at a tilt angle of 10 ° and exercising at a constant exercise speed and extending the exercise time by 5 minutes every day, 4 times as a basic unit. Was. When repeated, the exercise speed was increased by 5 m / min. That is, on the first day, 10 m / min for 30 minutes. On the second day, 10m / min, 35 minutes.
On the fifth day, 10m / min, 50 minutes. On the sixth day, 15m / min, 30
Minutes. On the seventh day, 15m / min, 35 minutes. It becomes 20 on the last day
On the day, they exercised 25 m / min for 50 minutes. Day 21 is 2
The time required for the rat to stop moving at a speed of 5 m / min was measured, and this time was used as the test data for motility. Tables 1 and 2 show the food consumption and the results. All of the hydrolyzed peptide groups derived from skin and bone collagen showed significantly higher motility than the control group. Therefore, it was found that ingestion of the hydrolyzed peptide derived from bone and skin collagen has a fatigue recovery effect.

[0051]

[Table 1]

[0052]

[Table 2]

Experimental Example 2: Nutrient tonic effect and fatigue recovery effect The protein component was degreased from pig skin tissue, treated with alkali, and then hydrolyzed with proteolytic enzymes to give an average molecular weight of about 25.
A bone collagen-derived hydrolyzate (trade name: GELITA-SOLM, manufactured by DGF) prepared to be 00 was used. The formulation used was Example 1 below. The subject,
Regardless of the season, people who tended to feel tired or tired in daily life were self-reported, and among them, 36 patients who did not receive medical treatment such as medication were used. The subject's age is 2
4 to 68 years old, average age 48 years, male and female ratio,
There were 16 men and 20 women. In the test, the subjects were divided into two groups, and the first two weeks were set as an observation period before drinking, the following four weeks were set as a drinking period, and the further four weeks were set as a follow-up observation period after the end of drinking. Judgments regarding changes in physical condition were self-reported. That is, every day, they were asked to fill in the physical condition felt several hours after waking up. As for the degree of physical condition, "I feel good" is 1 and "somehow lazy" is 3.
“I feel bad when I'm awake” 5
The evaluation was graded. The subject performed the same questionnaire twice immediately after the end of drinking and at the end of the follow-up after the end of drinking. The dose should be 5% of the amount of protein ingested per dose.
g. Drinking was done once a day, and in principle, it was taken after dinner until bedtime. Table 3 shows the drinking results. The condition of the drinking period of the hydrolyzed peptide derived from bone collagen was clearly better than the condition before and after the drinking. Therefore, protein intake is nutrient tonic,
And a fatigue recovery effect.

[0054]

[Table 3]

Experimental Example 3: Immunostimulatory effect
Blood should be collected immediately before the start of drinking and at the end of the 4-week drinking period.
NK cell activity was measured. That is, blood sampling sump
Heparin is added to the sample, and the effector is centrifuged at the specific gravity.
-Separate cells. 1 × 10 cells⁶Adjust to pieces / ml
Was. On the other hand, culture the RI-labeled target cells and
Adjusted to cell number. Dispense target cells into microplate
Further, IN-HCl was added to Group A, and 10% FB was added to Group B.
The effector cells were dispensed and centrifuged to groups S and C.
5% CO _TwoThe cells were cultured in an incubator for 4 hours. culture
Then, it is measured by a scintillation counter, and the equation (1)
The NK cell activity (%) was calculated. Table 4 shows the results.
You.

[0056]

(Equation 1)

[0057]

[Table 4]

The results showed that NK activity was clearly increased before and after drinking the hydrolyzed peptide derived from bone collagen. Therefore, it was found that protein intake had an immunostimulatory effect.

Example 1 Beverage Each component was mixed according to the following formulation to prepare a beverage according to a conventional method. Ingredients Amount (%) * Collagen hydrolyzate derived from pig skin 20.0 (average molecular weight 2500) Fructose 10.0 Eleuthero extract 1.0 Eucommia extract 1.0 Methylparaben 0.2 Thiamine nitrate 0.005 Sodium riboflavin phosphate 0.005 Pyridoxine hydrochloride 0.005 Perfume Appropriate amount Citric acid Appropriate amount Water balance Total 100.0 (pH 6.5, lipid content 0.1 g / 100 ml) The above beverage is 30 per day for the purpose of nutritional tonic and recovery from fatigue
You can drink about ml. * Collagen hydrolyzate derived from cow hide: GEKITA SOL M
(Manufactured by DGF)

Example 2 Beverage Each component was mixed according to the following formulation to prepare a beverage according to a conventional method. Ingredients Blended amount (%) Sucrose 8.0 Collagen hydrolyzate derived from tuna 5.0 (average molecular weight 2500) Pectin 2.0 Yellow perfused extract 0.5 (equivalent to 0.5% yellow perfused extract) Sodium ascorbate 0. 5 Aminoethylsulfonic acid 0.5 Caffeine anhydride 0.05 Nicotinamide 0.02 dl-α-tocopherol acetate 0.01 Calcium pantothenate 0.01 Appropriate amount of fragrance Citric acid Appropriate amount Water balance Total 100.0 (pH 5. 5. Lipid content 0.4g / 100ml) The above drink is 10 per day for the purpose of nourishing tonic and recovering from fatigue.
About 0 ml can be drunk.

Example 2: Tablet A tablet was prepared by mixing the components according to the following formulation and following a conventional method. Ingredients Blended amount (%) Hydrolyzate of collagen derived from cockscomb 50.0 (Average molecular weight 5000) Powdered reduced maltose starch syrup 25.0 Sucrose fatty acid ester 3.0 Lactose Residue Total 100.0 (Lipid content 0 g / 100 g) Tablet Is tableted to 300 mg, and about 10 tablets can be taken per day for the purpose of nutrition, tonicity and recovery from fatigue.

Example 3 Beverage Each component was mixed according to the following formulation to prepare a quasi-drug beverage according to a conventional method. Ingredients Amount (%) Glucose fructose syrup 10.0 Hydrolyzate of shark-derived collagen 3.0 (average molecular weight 2000) Carrot extract powder 1.0 Aminoethylsulfonic acid 1.0 Hawthorn extract 0.5 Royal jelly 0. 5 Anhydrous caffeine 0.5 Pyridoxine hydrochloride 0.01 Pyridoxine phosphate 0.01 Water balance Total 100.0 (pH 5.3, lipid content 0.2 g / 100 ml) The quasi-drug beverage obtained is 50 ml per day can be taken for the purpose of recovery from fatigue.

Example 4: Tablet A tablet was prepared according to a conventional method by mixing the components according to the following formulation. Ingredients Amount (%) Collagen hydrolyzate derived from salmon 35.0 Powder-reduced maltose starch syrup 30.0 Trehalose 10.0 Grape skin extracted polyphenol powder 3.0 Aspartame Trace amount of appropriate amount Lactose Residue Total 100.0 (Lipid content 0 0.3 g / 100 g) The above mixture was compressed into a 3000 mg disk-shaped tablet. The tablets obtained can be ingested about 2 tablets a day for the purpose of nutrition, tonicity and recovery from fatigue.

Example 5: Hard Capsules Each component was mixed according to the following formulation to prepare soft capsules according to a conventional method. Ingredients Blended amount (%) Casein hydrolyzate 90.0 (average molecular weight 10,000) Propolis extract 5.0 Garlic extract 5.0 Total 100.0 (lipid content 0.4 g / ml) Enclose 250 mg according to the method. The obtained hard capsule is used for 5 days a day for immunostimulation and prevention of infectious respiratory diseases.
About 10 capsules can be taken.

Example 6: Powdered beverage A powdered beverage was prepared according to a conventional method by mixing the components according to the following formulation. Ingredients Blended amount (%) Powdered separated soy protein 50.0 (average molecular weight 350,000) Sugar 10.0 Egg white powder 5.0 Skim cocoa powder 3.0 Powder flavor appropriate amount Indigestible dextrin Residual total 100.0 (lipid Content: 0.2 g / 100 g) For the purpose of immunostimulation and prevention of infectious respiratory diseases, 10 g per day is mixed with water, fruit juice, milk, etc. for drinking for the purpose of immunostimulation and prevention of infectious respiratory diseases.

Formulation Example 7: Jelly The components were mixed according to the following formulation, and a jelly was prepared according to a conventional method. Ingredients Blended amount (%) Collagen derived from bovine bone 20.0 (average molecular weight 3500) Oligosaccharide 15.0 Reduced starch syrup 10.0 * Hydrolyzed product of collagen derived from bovine bone 10.0 (average molecular weight 3000) Agar 5.0 echinacea extract 0.5 flavor 0.1 water balance total 100.0 (lipid content 0g / 100g) * Collagen hydrolyzate derived from bovine bone: GEKITA SOL D2
(Manufactured by DGF) The above composition is provided by filling about 5 g in a portion. Ingest two per day as a guide for immunostimulation and prevention of infectious respiratory diseases.

Formulation Example 7: Soup Each component was mixed according to the following formulation to prepare a soup according to a conventional method. Ingredients Blended amount (%) Tomato puree 10.0 Onion slices 5.0 Collagen-derived collagen hydrolysate 5.0 (average molecular weight 8000) Dried parsley powder, peas proper amount Lemon juice proper amount Seasoning (potassium chloride, pepper) ) Appropriate amount Seafood bouillon Appropriate amount Water balance Total 100.0 (lipid content 0.4g / 100ml) About 150g of the above composition is filled in a retort pouch,
Provided. Eat one bag per day as a guide for immunostimulation and prevention of infectious respiratory diseases.

[0068]

By taking the oral composition of the present invention, it is possible to obtain a nutritional tonic effect, a fatigue recovery effect, an immunostimulatory effect and a preventive effect of infectious respiratory diseases.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) A61K 9/08 A61K 31/197 4C083 31/197 31/4415 4C086 31/4415 31/51 4C087 31/51 31 / 525 4C206 31/525 35/20 35/20 A61P 3/02 38/17 11/00 A61P 3/02 37/04 11/00 A23L 1/06 37/04 1/39 // A23L 1/06 2 / 38 D 1/39 C 2/52 Z 2/38 A61K 37/18 37/16 A23L 2/00 FF term (reference) 4B017 LC03 LG15 LK15 LK16 4B018 LE05 MD20 MD23 MD61 ME02 4B036 LC06 LF01 LH15 LH17 LH34 4B041 LC10 LD02 LK13 LK16 LK32. AA01 AA 02 BB39 CA47 MA01 MA02 MA17 MA52 NA14 ZA59 ZA94 ZB09 ZC21 4C206 AA01 AA02 FA44 MA01 MA03 MA04 MA37 MA72 NA14 ZA59 ZA94 ZB09 ZC21

Claims (4)

[Claims] 1. An oral composition comprising at least one selected from a milk protein hydrolyzate, a collagen protein hydrolysate, a soy protein and a hydrolyzate thereof, and substantially free of lipids. 2. The oral composition according to claim 1, wherein the milk protein hydrolyzate, the collagen protein hydrolysate, the soybean protein and the hydrolyzate thereof have an average molecular weight of 200 to 10,000. 3. The oral composition according to claim 1, further comprising a crude drug and a vitamin. 4. The composition according to claim 1, wherein the oral composition is a liquid having a pH of 6.5 or less.