JP2006348050A - Medicine and health food composition for promoting bloodstream by vasolidation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new material that exhibits a vasolidative action thereby to suppress or alleviate various human diseases or disorders. <P>SOLUTION: The composition comprises as an effective ingredient a peptide obtained by hydrolyzing a protein derived from a milk protein selected from among skim milk powders and whey. The composition is used as a medicinal composition or a health food composition and exhibits vasolidation effects thereby to suppress or improve various symptoms such as stiff shoulders, headache or cold constitution associated with lowering of bloodstream. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a pharmaceutical and health food composition having a vasodilatory effect, thereby suppressing and improving stiff shoulders, headaches, coldness and related hypomorphic symptoms, and more particularly, derived from milk protein It is related with the said pharmaceutical and health food composition which use the peptide obtained by hydrolyzing protein of this as an active ingredient.

  Improving blood circulation leads to improvement of symptoms such as stiff shoulders, coolness, headache, numbness of limbs, recovery from fatigue, and promotion of end tissue and hair metabolism. Improvement of blood circulation as a whole, oxygen and nutrients are adequately distributed to the end tissues, and waste products such as carbon dioxide and lactic acid are collected, making it comfortable after bathing, warming the body, fatigue, neuralgia, climacteric disorder Symptoms such as disappearance are improved.

  There are several mechanisms that promote blood flow. One is an increase in blood fluidity by improving the deformability of red blood cells, and the other is an increase in blood flow by dilating blood vessels. As the prior art related to the former, there is, for example, a method that prevents a reduction in deformability of erythrocytes by jujube and its extract (see Patent Document 1 and Patent Document 2), and lowers the viscosity of blood components such as red blood cells, white blood cells, and platelets. There is also a report on a collagen peptide that improves blood fluidity (see Patent Document 3).

  Moreover, although the following various natural products or the extract of the blood flow improvement effect has been reported, the mechanism of the blood flow improvement is not necessarily clear. For example, saffron or saffron extract (see Patent Document 4), dandelion and mugwort extract (see Patent Document 5), boiled bovine viscera and bone (see Patent Document 6), lotus root knot Scallops of root hair (see patent document 7), water-soluble extract of purple sweet potato (see patent document 8), theanine-containing material (see patent document 9), ground yellow (see patent document 10), saponin of sesame seed (patent document) 11) is known.

In addition, as an example in which vasodilation is observed, it has been reported that a peptide derived from sardine muscle expands a blood vessel of a rabbit ear (see Patent Document 12). However, it is a report only on the phenomenon side, and does not describe a report of an effective example for a person or a use.
In recent years, further development of blood flow improving substances by various blood flow improving mechanisms has been demanded.
Japanese Patent Laid-Open No. 05-210639 JP 07-61933 A Japanese Patent No. 3197547 Japanese Patent Laid-Open No. 10-287576 JP-A-60-160856 JP 54-41354 A JP-A-53-133646 JP 2001-145471 A JP 2000-247878 A JP 2000-169385 A JP 07-233191 A Japanese Patent No. 2732056

  An object of the present invention is to provide a novel material that has an action of dilating blood vessels, thereby improving blood circulation, suppressing or reducing shoulder stiffness, coldness, headache, fatigue, climacteric disorder, and promoting metabolism. Furthermore, an object of the present invention is to provide health foods and pharmaceuticals having such actions.

  As a result of screening the components mainly composed of peptides obtained by hydrolyzing a protein derived from milk protein selected from skim milk powder and whey using blood vessels of rabbit ears, these peptides It has been found that there is an effect of dilating blood vessels. That is, components containing peptides obtained by hydrolysis of the protein with acid or alkali and / or hydrolysis with enzyme protease were orally purified as they were orally administered to rabbits to confirm vascular dilation.

  Vasodilation observed in the ears of these rabbits leads to increased blood flow, resulting in blood pressure lowering effects, stiff shoulders, cooling and headache improvement effects, fatigue recovery effects, climacteric disorder suppression and alleviation effects, metabolic effects A wide range of effects were confirmed, including hair-growth effect by promotion and skin condition improvement effect.

  Therefore, the present invention relates to a blood flow-promoting pharmaceutical and health food composition by vasodilation, which comprises a peptide obtained by hydrolysis of the protein as an active ingredient.

  In the present invention, protein hydrolysis is generally performed by acid or alkali decomposition or enzymatic decomposition using a protease. As the acid or alkali, any of organic and inorganic acids and alkalis may be used. In the case of acid hydrolysis, a pH of 1 to 4 is preferable, and in the case of alkaline hydrolysis, a pH of 8 to 13 is preferable. Reaction conditions such as temperature and time during decomposition are appropriately set.

  Proteases include pepsin, pancreatin, papain, pro leather (manufactured by Amano Pharmaceutical Co., Ltd.), Samoaase (manufactured by Daiwa Kasei Co., Ltd.), Sumiteam AP, Sumiteam MP, Sumiteam FP (all manufactured by Shin Nippon Chemical Co., Ltd.), and other general An enzyme having protease activity used in the above can be used. In the reaction, the optimum concentration of the enzyme, reaction pH, reaction temperature, and other conditions may be selected for each enzyme agent.

  In hydrolysis with acid or alkali, it is not always easy to adjust the progress of the reaction to a certain level. Therefore, strict reaction management is necessary. Furthermore, hydrolysis treatment with an acid or alkali and hydrolysis with an enzyme can be used in combination. Subsequent treatment with an acid or alkali followed by proteolytic enzyme treatment leads to a reduction in the molecular weight of the peptide and an increase in the proportion of low molecular weight peptides. Since a low molecular weight peptide is easily absorbed in the digestive tract and has a high vasodilator effect, it is desirable to increase the ratio of the low molecular weight peptide in this way. However, in the human gastrointestinal tract, high-molecular peptides also generate low-molecular-weight peptides by pepsin, trypsin, and peptidase in the gastrointestinal tract. However, it is considered that the effect of the low molecular peptide is surely exhibited in a short time. Therefore, the molecular weight of the peptide is preferably higher in the ratio of the low molecular weight peptide, but when used as a food, the effect of vasodilation is recognized even if the polymer peptide is contained.

  As the peptide component obtained by hydrolysis of each protein, the reaction product may be used as it is, or the peptide component may be concentrated and purified. The concentration and purification treatment can be performed by a desalting treatment using an electrodialysis membrane, a desalting / concentration treatment using an ion exchange resin, a decolorization using activated carbon, a deodorizing and concentration treatment, or a precipitation treatment using an organic solvent. Each component may be used as it is, but can also be pulverized by spray drying or freeze drying. Moreover, what was further purified using chromatography can also be used.

  The milk protein raw material can be used as it is as the protein raw material to be hydrolyzed. However, if the raw material has a large amount of fat, the fat remains in the decomposed product and is oxidized to generate a strange odor or taste. Therefore, it is desirable that the protein be purified. Therefore, it is desirable to use raw materials from which oil has been separated in advance.

  The confirmation of the vasodilator effect was performed using rabbit ears. That is, a sample was orally administered to a rabbit, the change of the ear blood vessel was visually confirmed, and the degree of vasodilation was quantified using the area calculation software from the photograph. In all the examples shown below, the vasodilator effect was confirmed by this method.

  In the present invention, vascular vasodilation by a protein hydrolyzate derived from milk protein results in increased blood flow, thereby improving the transfer of nutrients to the end tissue and improving the transfer of waste products. Estimated. As a result, it can be used for the purpose of suppressing or reducing stiff shoulders, headaches, and coldness in humans. In addition, improvement of blood circulation can improve physiological functions related to not only the circulatory system but also the nervous system, endocrine system, and immune system, and can be used for the purpose of suppressing and improving sleep disorders and climacteric disorders. In addition, carbon dioxide, lactic acid and other waste products from peripheral tissues are collected as appropriate, and oxygen and nutritional components are sufficiently supplied to the peripheral tissues, so that fatigue recovery, skin condition improvement, cosmetic effects, and hair growth effects are achieved. Can also be obtained. About these effects, the result of the test in a human was shown as an Example.

  As a use aspect of the said milk protein origin protein hydrolyzate of this invention, it can add to a general food as a food additive, and can be used as a health food composition or a pharmaceutical composition. The form of the protein hydrolyzate may be any of an aqueous solution, a suspension, a powder, a solid molding, and the like, and is not particularly limited. Therefore, it can be used in a wide range of general foods, and as health foods and pharmaceuticals, it is provided as capsules, tablets, powders, granules, drinks and the like. In that case, it can be used not only as a simple ingredient, but also in combination with other taste ingredients, bulking agents, stabilizers, and functional materials such as herbal medicines and herbs and their functional ingredients, Moreover, it can also be used by mixing with nutrient components such as vitamins and minerals, and materials acceptable as food.

  The dose for humans to exert these effects is preferably 0.5 to 2000 mg / kg · day, more preferably 10 to 400 mg / kg · day as a protein hydrolyzate. However, since there are individual differences in the type and degree of symptoms, it is not limited to this range.

  According to the present invention, a composition containing a peptide obtained by hydrolyzing milk protein selected from skim milk powder and whey can be used for pharmaceuticals and health foods to exert a vasodilator effect, Can suppress and ameliorate stiff shoulders, headaches, coldness and related symptoms of hypofunction.

Embodiments of the present invention will be described below with reference to production examples and examples.
(Production Example 1)
As an example of protein hydrolyzate derived from livestock milk, each peptide was obtained from skim milk powder and whey as follows. Commercially available skim milk powder and 1 kg of commercially available separated whey protein were each suspended in 2 liters of warm water, adjusted to pH 7.5, 40 g of Samoaze (manufactured by Daiwa Kasei Co., Ltd.) was added to each, and reacted at 50 ° C for 16 hours. . After the reaction, the enzyme was inactivated by heating at 100 ° C. for 10 minutes, adsorbed on Dowex 50 (H ⁺ ), washed with water, and eluted with aqueous ammonia to obtain a peptide segment. After concentration under reduced pressure to remove ammonia, lyophilized to obtain 270 g (sample 1) of skim milk powder peptide and 333 g of separated whey protein peptide (sample 2).

  Next, examples and usage examples in which the vasodilator effect of each sample obtained in the above production example was confirmed will be shown.

Example 1
A 9-week-old male rabbit (Slc: JW · CSK) weighing 1.5 to 1.8 kg was subjected to preliminary breeding for one week and then subjected to the experiment. The breeding room had a temperature of 22 ° C. ± 1 ° C., a humidity of 50%, and a light / dark cycle of 12 hours cycle. The sample was fasted for 2 hours before giving the sample. A sample of 1000 mg per kg body weight was dissolved in 3 to 10 ml of physiological saline and forcibly ingested with an oral sonde.

  The blood vessel state of the rabbit ear was measured as follows. After fixing the rabbit in the blood collection box, when the rabbit settled down and no change in the ear blood vessel was observed, the digital camera was used to take a picture, and this time was set to 0 minutes. Photographing was started 10 minutes after the sample was ingested, and photographing was continued every 10 minutes until the dilation of the blood vessels subsided. The obtained photograph was subjected to area calculation with area calculation software LIA32 and digitized.

  The above measurement was performed on Samples 1 and 2 obtained in Production Example 1, and the results were graphed and shown in FIG. In the figure, the measured blood vessel cross-sectional area on the vertical axis is shown as a numerical value with 1 as the case of 0 minutes, and the change after the lapse of time is illustrated. As a result, it was confirmed that any sample was used to have an ear vasodilatory effect in rabbits.

(Example 2)
300 mg weight tablets each containing 80% of Samples 1 and 2, 10% of reduced maltose starch syrup, 7% dextrin, and 3% sucrose fatty acid ester (both by weight) were produced.

(Example 3)
The temperature of the fingertips after taking the tablets containing Samples 1-2 described in Example 2 was measured. In the test, 10 volunteers were selected from those who were effective in relieving or alleviating coldness among volunteers, and they were tested over multiple days. The test was conducted without breakfast, after entering the room adjusted to 20 ° C and humidity 50%, after 1 hour, and taking 6 tablets each containing 1 to 2 samples with 50 ml of water and changing the fingertip temperature. Was measured with a thermistor thermometer after 30 minutes of dosing, and expressed as the temperature rising from 0 hours. Two volunteers were tested for one sample, and the average value was recorded.

The graph which shows the experimental data of the vasodilatory effect | action of the peptide derived from milk performed about the ear of the rabbit.

Claims (2)

  A pharmaceutical composition for promoting blood flow by vasodilation, comprising as an active ingredient a peptide obtained by hydrolyzing a protein derived from milk protein selected from skim milk powder and whey.   A blood flow promoting health food composition by vasodilation comprising a peptide obtained by hydrolyzing a protein derived from milk protein selected from skim milk powder and whey.

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