RU2652875C2 - Сконструированные микробы и способы получения микробного масла - Google Patents

Сконструированные микробы и способы получения микробного масла Download PDF

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RU2652875C2
RU2652875C2 RU2014119784A RU2014119784A RU2652875C2 RU 2652875 C2 RU2652875 C2 RU 2652875C2 RU 2014119784 A RU2014119784 A RU 2014119784A RU 2014119784 A RU2014119784 A RU 2014119784A RU 2652875 C2 RU2652875 C2 RU 2652875C2
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yeast cell
fatty acid
isolated fat
forming yeast
nucleic acid
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RU2014119784A
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RU2014119784A (ru
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Грегори СТЕФАНОПУЛОС
Митчел ТАЙ
Сагар ЧАКРАБОРТИ
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Массачусетс Инститьют Оф Текнолоджи
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    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
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    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03006—Citrate (pro-3S)-lyase (4.1.3.6)
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03008—ATP citrate synthase (2.3.3.8)
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    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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RU2014119784A 2011-10-19 2012-10-19 Сконструированные микробы и способы получения микробного масла RU2652875C2 (ru)

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Application Number Priority Date Filing Date Title
US201161548901P 2011-10-19 2011-10-19
US61/548,901 2011-10-19
US201261663263P 2012-06-22 2012-06-22
US61/663,263 2012-06-22
PCT/US2012/061101 WO2013059649A1 (en) 2011-10-19 2012-10-19 Engineered microbes and methods for microbial oil production

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RU2652875C2 true RU2652875C2 (ru) 2018-05-03

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US (2) US8951776B2 (enExample)
EP (2) EP3388516A1 (enExample)
JP (1) JP6294827B2 (enExample)
KR (1) KR102036389B1 (enExample)
CN (2) CN104160020A (enExample)
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RU2799537C1 (ru) * 2022-09-07 2023-07-05 Федеральное государственное бюджетное учреждение науки Федеральный исследовательский центр "Институт биологии южных морей имени А.О. Ковалевского РАН" (ФИЦ ИнБЮМ) Способ получения растительного масла с повышенной биологической ценностью

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US9862977B2 (en) 2018-01-09
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