JP6294827B2 - 操作された微生物および微生物油生成のための方法 - Google Patents

操作された微生物および微生物油生成のための方法 Download PDF

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JP6294827B2
JP6294827B2 JP2014537306A JP2014537306A JP6294827B2 JP 6294827 B2 JP6294827 B2 JP 6294827B2 JP 2014537306 A JP2014537306 A JP 2014537306A JP 2014537306 A JP2014537306 A JP 2014537306A JP 6294827 B2 JP6294827 B2 JP 6294827B2
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lipid
fatty acid
cell
nucleic acid
expression
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JP2014530626A5 (enExample
JP2014530626A (ja
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ステファノプロス，グレゴリー
タイ，ミッチェル
チャクラボルティー，サガール
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Massachusetts Institute of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0097—Oxidoreductases (1.) acting on reduced flavodoxin as donor (1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03006—Citrate (pro-3S)-lyase (4.1.3.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03008—ATP citrate synthase (2.3.3.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2014537306A 2011-10-19 2012-10-19 操作された微生物および微生物油生成のための方法 Expired - Fee Related JP6294827B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161548901P 2011-10-19 2011-10-19
US61/548,901 2011-10-19
US201261663263P 2012-06-22 2012-06-22
US61/663,263 2012-06-22
PCT/US2012/061101 WO2013059649A1 (en) 2011-10-19 2012-10-19 Engineered microbes and methods for microbial oil production

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JP2014530626A JP2014530626A (ja) 2014-11-20
JP2014530626A5 JP2014530626A5 (enExample) 2015-12-10
JP6294827B2 true JP6294827B2 (ja) 2018-03-14

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US (2) US8951776B2 (enExample)
EP (2) EP3388516A1 (enExample)
JP (1) JP6294827B2 (enExample)
KR (1) KR102036389B1 (enExample)
CN (2) CN104160020A (enExample)
BR (1) BR112014009245A2 (enExample)
RU (1) RU2652875C2 (enExample)
WO (1) WO2013059649A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011109548A2 (en) * 2010-03-02 2011-09-09 Massachusetts Institute Of Technology Microbial engineering for the production of fatty acids and fatty acid derivatives
EP3388516A1 (en) 2011-10-19 2018-10-17 Massachusetts Institute Of Technology Engineered microbes and methods for microbial oil production
MX2014011979A (es) * 2012-04-09 2015-01-16 Bp Biofuels Uk Ltd Microorganismos bajos en polisacaridos para la produccion de biocombustibles y otros materiales renovables.
CN105431529A (zh) * 2013-05-03 2016-03-23 德克萨斯大学系统董事会 用于真菌脂质生产的组合物和方法
EP3137616B1 (en) * 2014-05-01 2023-05-31 Ginkgo Bioworks, Inc. Increasing cellular lipid production by increasingthe activity of diacylglycerol acyltransferase and decreasing the activity of triacylglycerol lipase
WO2015184303A1 (en) * 2014-05-29 2015-12-03 Novogy, Inc. Increasing lipid production in oleaginous yeast
KR102311681B1 (ko) 2015-07-28 2021-10-12 삼성전자주식회사 내산성을 갖는 효모 세포, 그를 이용하여 유기산을 생산하는 방법 및 상기 내산성 효모 세포를 생산하는 방법
JP2017039078A (ja) * 2015-08-19 2017-02-23 太平洋セメント株式会社 排水の処理方法
US11142770B2 (en) 2015-10-20 2021-10-12 Massachusetts Institute Of Technology Isolated oleaginous yeast
EP3652300A4 (en) 2017-07-13 2021-06-23 Radici Chimica S.p.A. BIOLOGICAL PROCEDURES FOR MODIFYING CELLULAR CARBON FLOW
CN107904199A (zh) * 2017-11-30 2018-04-13 杭州唯铂莱生物科技有限公司 用于合成山扁豆酸的工程改造的宿主细胞和方法
CN110358692B (zh) * 2018-04-09 2021-07-27 中国科学院青岛生物能源与过程研究所 生产神经酸的重组酵母菌株及其应用
WO2019241322A1 (en) * 2018-06-13 2019-12-19 Manus Bio, Inc. Enzymes, methods, and host cells for producing carminic acid
CN109929870B (zh) * 2019-02-20 2021-03-16 天津大学 糖代谢与脂质代谢协同提高解脂耶氏酵母合成脂肪酸衍生物的产量的应用
GB202010630D0 (en) * 2020-07-10 2020-08-26 Imp College Innovations Ltd Methods
WO2024097353A1 (en) * 2022-11-04 2024-05-10 Viridos, Inc. Recombinant algae having high biomass and lipid productivity
WO2025229117A1 (en) 2024-05-02 2025-11-06 Vib Vzw Yeast for increased lipid production
CN119799810A (zh) * 2025-03-14 2025-04-11 山西鑫晋商生物科技有限公司 基于发酵工艺优化的二酯油微生物发酵方法及系统

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUP0200480A3 (en) * 1999-04-01 2009-08-28 Basf Plant Science Gmbh Enzymes of the biosynthetic pathway for the production of triacylglycerol and recombinant dna molecules encoding these enzymes
US7267976B2 (en) * 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7202356B2 (en) * 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
AU2005250074B2 (en) * 2004-06-04 2011-04-14 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
US7198937B2 (en) * 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7273746B2 (en) * 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
EP2166099B1 (en) 2005-03-08 2012-12-19 BASF Plant Science GmbH Expression enhancing intron sequences
EP2730656A1 (en) * 2007-05-24 2014-05-14 E. I. du Pont de Nemours and Company Soybean meal from beans having DGAT genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean
US20100317073A1 (en) 2007-12-04 2010-12-16 The Ohio State University Research Foundation Molecular approaches for the optimization of biofuel production
EP2324120B1 (en) * 2008-08-29 2016-01-27 E. I. du Pont de Nemours and Company Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms
AU2009308236B2 (en) * 2008-10-23 2016-06-30 Lumen Bioscience, Inc. Modified photosynthetic microorganisms for producing triglycerides
US8835137B2 (en) 2008-12-23 2014-09-16 Matrix Genetics, Llc Modified photosynthetic microorganisms with reduced glycogen and their use in producing carbon-based products
WO2011026008A1 (en) 2009-08-28 2011-03-03 Phycal Llc Biofuel from recombinant oleginous algae using sugar carbon sources
BR112012015641A2 (pt) * 2009-12-24 2015-11-24 Du Pont "polinucleotídeo isolado, construtor de dna recombinante, célula hospedeira, método para produzir uma planta transformada, para aumentar o teor de pelo menos um um ácido graxo, para aumentar pelo menos uma eficiencia de conversão, semente transgênica, plantas, óleos, alimento humano ou de animal e plantas de progênie"
US20110177564A1 (en) 2010-01-15 2011-07-21 Massachusetts Institute Of Technology Bioprocess and microbe engineering for total carbon utilization in biofuel production
WO2011109548A2 (en) * 2010-03-02 2011-09-09 Massachusetts Institute Of Technology Microbial engineering for the production of fatty acids and fatty acid derivatives
TW201144442A (en) 2010-05-17 2011-12-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
AU2011349444B2 (en) 2010-12-20 2016-06-30 Lumen Bioscience, Inc. Modified photosynthetic microorganisms for producing lipids
EP2655592A2 (en) 2010-12-20 2013-10-30 Matrix Genetics, LLC Modified photosynthetic microorganisms for producing lipids
TW201307553A (zh) 2011-07-26 2013-02-16 Dow Agrosciences Llc 在植物中生產二十二碳六烯酸（ｄｈａ）及其他長鏈多元不飽和脂肪酸（ｌｃ－ｐｕｆａ）之技術
EP3388516A1 (en) * 2011-10-19 2018-10-17 Massachusetts Institute Of Technology Engineered microbes and methods for microbial oil production
US20150329868A1 (en) * 2012-02-03 2015-11-19 Matrix Genetics, Llc Modified photosynthetic microorganisms for continuous production of carbon-containing compounds
WO2013134139A1 (en) * 2012-03-09 2013-09-12 Matrix Genetics, Llc Modified diacylglycerol acyltransferase proteins and methods of use thereof
US9096876B2 (en) 2012-06-22 2015-08-04 Massachusetts Institute Of Technology Engineered microbes and methods for microbial oil overproduction from cellulosic materials

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EP2768954A1 (en) 2014-08-27
US20130143282A1 (en) 2013-06-06
KR20140091004A (ko) 2014-07-18
US8951776B2 (en) 2015-02-10
US20150167034A1 (en) 2015-06-18
CN104160020A (zh) 2014-11-19
KR102036389B1 (ko) 2019-10-24
US9862977B2 (en) 2018-01-09
CN109468241A (zh) 2019-03-15
BR112014009245A2 (pt) 2020-10-27
JP2014530626A (ja) 2014-11-20
EP2768954B1 (en) 2018-12-05
RU2652875C2 (ru) 2018-05-03
EP2768954A4 (en) 2015-09-23
RU2014119784A (ru) 2015-11-27
EP3388516A1 (en) 2018-10-17
WO2013059649A1 (en) 2013-04-25

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