RU2222598C2 - Вектор для экспрессии гетерологичного белка, плазмида (варианты) - Google Patents

Вектор для экспрессии гетерологичного белка, плазмида (варианты) Download PDF

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RU2222598C2
RU2222598C2 RU2000102364/13A RU2000102364A RU2222598C2 RU 2222598 C2 RU2222598 C2 RU 2222598C2 RU 2000102364/13 A RU2000102364/13 A RU 2000102364/13A RU 2000102364 A RU2000102364 A RU 2000102364A RU 2222598 C2 RU2222598 C2 RU 2222598C2
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vector according
site
sequence encoding
vector
heterologous protein
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RU2000102364A (ru
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Спартаку АСТОЛФИ-ФИЛЬЮ (BR)
Спартаку АСТОЛФИ-ФИЛЬЮ
ЛИМА Биатрис Долабела ДИ (BR)
ЛИМА Биатрис Долабела ДИ
Джозеф Эрнст ТИМАНН (BR)
Джозеф Эрнст ТИМАНН
СОЗА Элоиза Рибейру Тунес ДИ (BR)
СОЗА Элоиза Рибейру Тунес ДИ
Люсиано ВИЛЕЛА (BR)
Люсиано ВИЛЕЛА
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Универсидади Ди Бразилиа
Биобрас С.А.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
RU2000102364/13A 1997-07-02 1998-07-02 Вектор для экспрессии гетерологичного белка, плазмида (варианты) RU2222598C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/886,967 1997-07-02
US08/886,967 US6068993A (en) 1997-07-02 1997-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin

Publications (2)

Publication Number Publication Date
RU2000102364A RU2000102364A (ru) 2002-03-27
RU2222598C2 true RU2222598C2 (ru) 2004-01-27

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US (4) US6068993A (enrdf_load_stackoverflow)
EP (1) EP0996713B1 (enrdf_load_stackoverflow)
AT (1) ATE278776T1 (enrdf_load_stackoverflow)
BR (1) BR9810650B1 (enrdf_load_stackoverflow)
CA (2) CA2687542C (enrdf_load_stackoverflow)
DE (1) DE69826864T2 (enrdf_load_stackoverflow)
IL (2) IL133832A0 (enrdf_load_stackoverflow)
IN (1) IN187721B (enrdf_load_stackoverflow)
PL (1) PL194141B1 (enrdf_load_stackoverflow)
RU (1) RU2222598C2 (enrdf_load_stackoverflow)
WO (1) WO1999001548A2 (enrdf_load_stackoverflow)

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US7049286B2 (en) * 2001-08-30 2006-05-23 Diatos, S.A. Insulin conjugates and methods of use thereof
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WO2005030185A2 (en) 2003-09-26 2005-04-07 Japan Tobacco Inc. Method of inhibiting remnant lipoprotein production
RU2263147C1 (ru) * 2004-02-16 2005-10-27 Открытое акционерное общество "Национальные биотехнологии" РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pHINS05, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД, СОДЕРЖАЩИЙ ПРОИНСУЛИН ЧЕЛОВЕКА, КЛЕТКА ESCHERICHIA COLI, ТРАНСФОРМИРОВАННАЯ РЕКОМБИНАНТНОЙ ПЛАЗМИДНОЙ ДНК pHINS05, И ШТАММ БАКТЕРИЙ ESCHERICHIA COLI JM109/pHINS05 - ПРОДУЦЕНТ ГИБРИДНОГО ПОЛИПЕПТИДА, СОДЕРЖАЩЕГО ПРОИНСУЛИН ЧЕЛОВЕКА
US7674621B2 (en) 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
EP1773878B1 (en) 2004-07-19 2015-03-04 Biocon Limited Insulin-oligomer conjugates, formulations and uses thereof
EP1797186B1 (en) * 2004-10-04 2016-05-25 National Research Council Of Canada Expression system, components thereof and methods of use
US7935788B2 (en) * 2004-10-04 2011-05-03 National Research Council Of Canada Reverse cumate repressor mutant
US8986997B2 (en) * 2007-08-31 2015-03-24 The Regents Of The University Of Michigan Methods and compositions for increasing biological molecule stability
DK2203181T3 (en) 2007-10-16 2018-05-28 Biocon Ltd An orally administrable solid composition and a method thereof
JP6081908B2 (ja) * 2010-03-31 2017-02-15 アーエムシルク ゲーエムベーハー 不溶性の標的タンパク質の分離
RU2497118C1 (ru) * 2012-03-06 2013-10-27 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) БЫСТРЫЙ СПОСОБ ОБРАБОТКИ ГЛАДКОМЫШЕЧНЫХ КЛЕТОК КИШЕЧНИКА ГЕМОЛИЗИНОМ II Bacillus cereus
CN102617703A (zh) * 2012-04-14 2012-08-01 拜明(苏州)生物技术有限公司 一种无需裂解高效提取发酵细菌中外源表达蛋白的方法
WO2015176067A2 (en) 2014-05-16 2015-11-19 The Regents Of The University Of California A long non-coding rna expressed in aggressive cancer
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds

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US5102789A (en) * 1989-03-15 1992-04-07 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of epideramal growth factor in pichia pastoris yeast cells
WO1991011518A1 (fr) * 1990-01-31 1991-08-08 Rhone-Poulenc Biochimie Polypeptides impliques dans la biosynthese des cobalamines et/ou des cobamides, sequences d'adn codant pour ces polypeptides, procede de preparation, et leur utilisation
RU2045575C1 (ru) * 1992-01-29 1995-10-10 Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Рекомбинатная плазмидная днк ppr - tgatg - hil - 1 beta - tsr, обеспечивающая синтез рекомбинантного интерлейкина-1 и штамм escherichia coli - продуцент рекомбинантного интерлейкина-1 бета человека
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Also Published As

Publication number Publication date
PL194141B1 (pl) 2007-04-30
CA2294760A1 (en) 1999-01-14
BR9810650B1 (pt) 2010-12-14
WO1999001548A2 (en) 1999-01-14
IL133832A0 (en) 2001-04-30
ATE278776T1 (de) 2004-10-15
US6068993A (en) 2000-05-30
US6509452B1 (en) 2003-01-21
EP0996713B1 (en) 2004-10-06
WO1999001548A3 (en) 1999-09-10
IN187721B (enrdf_load_stackoverflow) 2002-06-15
US6281329B1 (en) 2001-08-28
CA2687542A1 (en) 1999-01-14
IL133832A (en) 2007-07-04
DE69826864D1 (de) 2004-11-11
EP0996713A1 (en) 2000-05-03
PL348669A1 (en) 2002-06-03
CA2687542C (en) 2013-04-30
DE69826864T2 (de) 2005-11-03
CA2294760C (en) 2010-02-23
US6699692B2 (en) 2004-03-02
BR9810650A (pt) 2002-07-23
US20030082742A1 (en) 2003-05-01

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