IL133832A0 - Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin - Google Patents

Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin

Info

Publication number
IL133832A0
IL133832A0 IL13383298A IL13383298A IL133832A0 IL 133832 A0 IL133832 A0 IL 133832A0 IL 13383298 A IL13383298 A IL 13383298A IL 13383298 A IL13383298 A IL 13383298A IL 133832 A0 IL133832 A0 IL 133832A0
Authority
IL
Israel
Prior art keywords
insulin
protein
vector
recombinant
heterologous protein
Prior art date
Application number
IL13383298A
Other languages
English (en)
Original Assignee
Univ Brasilia
Biobras S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brasilia, Biobras S A filed Critical Univ Brasilia
Publication of IL133832A0 publication Critical patent/IL133832A0/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL13383298A 1997-07-02 1998-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin IL133832A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/886,967 US6068993A (en) 1997-07-02 1997-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
PCT/IB1998/001023 WO1999001548A2 (en) 1997-07-02 1998-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin

Publications (1)

Publication Number Publication Date
IL133832A0 true IL133832A0 (en) 2001-04-30

Family

ID=25390172

Family Applications (2)

Application Number Title Priority Date Filing Date
IL13383298A IL133832A0 (en) 1997-07-02 1998-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
IL133832A IL133832A (en) 1997-07-02 1999-12-30 Vector for the transformation of negative gram bacteria and the process for extracting the heterologous protein from the vector

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL133832A IL133832A (en) 1997-07-02 1999-12-30 Vector for the transformation of negative gram bacteria and the process for extracting the heterologous protein from the vector

Country Status (11)

Country Link
US (4) US6068993A (xx)
EP (1) EP0996713B1 (xx)
AT (1) ATE278776T1 (xx)
BR (1) BR9810650B1 (xx)
CA (2) CA2294760C (xx)
DE (1) DE69826864T2 (xx)
IL (2) IL133832A0 (xx)
IN (1) IN187721B (xx)
PL (1) PL194141B1 (xx)
RU (1) RU2222598C2 (xx)
WO (1) WO1999001548A2 (xx)

Families Citing this family (14)

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Publication number Priority date Publication date Assignee Title
US7049286B2 (en) * 2001-08-30 2006-05-23 Diatos, S.A. Insulin conjugates and methods of use thereof
WO2004042003A2 (en) * 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit
SG146695A1 (en) 2003-09-26 2008-10-30 Japan Tobacco Inc Method of inhibiting remnant lipoprotein production
US7674621B2 (en) * 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
EP2626368B1 (en) 2004-07-19 2016-12-21 Biocon Limited Insulin-oligomer conjugates, formulations and uses thereof
US7935788B2 (en) * 2004-10-04 2011-05-03 National Research Council Of Canada Reverse cumate repressor mutant
EP1797186B1 (en) * 2004-10-04 2016-05-25 National Research Council Of Canada Expression system, components thereof and methods of use
WO2009032664A2 (en) * 2007-08-31 2009-03-12 The Regents Of The University Of Michigan Methods and compositions for increasing biological molecule stability
RU2453332C2 (ru) 2007-10-16 2012-06-20 Байокон Лимитид Твердая фармацевтическая композиция (варианты) и способ контроля концентрации глюкозы с ее помощью, способ получения твердой фармацевтической композиции (варианты), таблетка (варианты) и способ получения амфорных частиц
EP2552935B1 (en) * 2010-03-31 2020-05-27 AMSilk GmbH Separation of insoluble target proteins
RU2497118C1 (ru) * 2012-03-06 2013-10-27 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) БЫСТРЫЙ СПОСОБ ОБРАБОТКИ ГЛАДКОМЫШЕЧНЫХ КЛЕТОК КИШЕЧНИКА ГЕМОЛИЗИНОМ II Bacillus cereus
CN102617703A (zh) * 2012-04-14 2012-08-01 拜明(苏州)生物技术有限公司 一种无需裂解高效提取发酵细菌中外源表达蛋白的方法
WO2015176067A2 (en) 2014-05-16 2015-11-19 The Regents Of The University Of California A long non-coding rna expressed in aggressive cancer
US10745457B2 (en) 2015-09-02 2020-08-18 Merck Sharp & Dohme Corp. Process for obtaining insulin with correctly formed disulfide bonds

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US4624926A (en) * 1981-01-02 1986-11-25 The Research Foundation Of State University Of New York Novel cloning vehicles for polypeptide expression in microbial hosts
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US4503155A (en) * 1982-02-01 1985-03-05 Eli Lilly And Company Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli
US4732859A (en) * 1982-09-03 1988-03-22 Eli Lilly And Company Method for conferring bacteriophage resistance to bacteria
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4578355A (en) * 1983-01-12 1986-03-25 The United States Of America As Represented By The Department Of Health And Human Services Plasmid cloning vector pAS1
US5256546A (en) * 1983-07-15 1993-10-26 Bio-Technology General Corp. Bacterial expression of porcine growth hormone
US4734368A (en) * 1984-03-23 1988-03-29 Huels Aktiengesellschaft Process for the bioconversion of fumarate to L-malate
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Also Published As

Publication number Publication date
IL133832A (en) 2007-07-04
US6281329B1 (en) 2001-08-28
BR9810650A (pt) 2002-07-23
IN187721B (xx) 2002-06-15
US6699692B2 (en) 2004-03-02
US6509452B1 (en) 2003-01-21
BR9810650B1 (pt) 2010-12-14
EP0996713B1 (en) 2004-10-06
US20030082742A1 (en) 2003-05-01
PL348669A1 (en) 2002-06-03
RU2222598C2 (ru) 2004-01-27
CA2687542C (en) 2013-04-30
PL194141B1 (pl) 2007-04-30
US6068993A (en) 2000-05-30
ATE278776T1 (de) 2004-10-15
DE69826864D1 (de) 2004-11-11
WO1999001548A2 (en) 1999-01-14
CA2687542A1 (en) 1999-01-14
WO1999001548A3 (en) 1999-09-10
CA2294760A1 (en) 1999-01-14
CA2294760C (en) 2010-02-23
EP0996713A1 (en) 2000-05-03
DE69826864T2 (de) 2005-11-03

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