DE69826864D1 - Vektor zur heterologen proteinexpression und verfahren zur extraktion rekombinanter proteine sowie zur isolierung rekombinanten insulins - Google Patents

Vektor zur heterologen proteinexpression und verfahren zur extraktion rekombinanter proteine sowie zur isolierung rekombinanten insulins

Info

Publication number
DE69826864D1
DE69826864D1 DE69826864T DE69826864T DE69826864D1 DE 69826864 D1 DE69826864 D1 DE 69826864D1 DE 69826864 T DE69826864 T DE 69826864T DE 69826864 T DE69826864 T DE 69826864T DE 69826864 D1 DE69826864 D1 DE 69826864D1
Authority
DE
Germany
Prior art keywords
vector
recombinant
insulin
pro
recombinant human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69826864T
Other languages
English (en)
Other versions
DE69826864T2 (de
Inventor
Spartaco Astolfi-Filho
Lima Dolabela De
Ernst Thiemann
Sousa Ribeiro De
Luciano Vilela
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biobras SA
Universidade de Brasilia
Original Assignee
Biobras SA
Universidade de Brasilia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biobras SA, Universidade de Brasilia filed Critical Biobras SA
Publication of DE69826864D1 publication Critical patent/DE69826864D1/de
Application granted granted Critical
Publication of DE69826864T2 publication Critical patent/DE69826864T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69826864T 1997-07-02 1998-07-02 Vektor zur heterologen proteinexpression und verfahren zur extraktion rekombinanter proteine sowie zur isolierung rekombinanten insulins Expired - Lifetime DE69826864T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/886,967 US6068993A (en) 1997-07-02 1997-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
US886967 1997-07-02
PCT/IB1998/001023 WO1999001548A2 (en) 1997-07-02 1998-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin

Publications (2)

Publication Number Publication Date
DE69826864D1 true DE69826864D1 (de) 2004-11-11
DE69826864T2 DE69826864T2 (de) 2005-11-03

Family

ID=25390172

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69826864T Expired - Lifetime DE69826864T2 (de) 1997-07-02 1998-07-02 Vektor zur heterologen proteinexpression und verfahren zur extraktion rekombinanter proteine sowie zur isolierung rekombinanten insulins

Country Status (11)

Country Link
US (4) US6068993A (de)
EP (1) EP0996713B1 (de)
AT (1) ATE278776T1 (de)
BR (1) BR9810650B1 (de)
CA (2) CA2294760C (de)
DE (1) DE69826864T2 (de)
IL (2) IL133832A0 (de)
IN (1) IN187721B (de)
PL (1) PL194141B1 (de)
RU (1) RU2222598C2 (de)
WO (1) WO1999001548A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7049286B2 (en) * 2001-08-30 2006-05-23 Diatos, S.A. Insulin conjugates and methods of use thereof
WO2004042003A2 (en) * 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit
SG146695A1 (en) 2003-09-26 2008-10-30 Japan Tobacco Inc Method of inhibiting remnant lipoprotein production
US7674621B2 (en) * 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
EP2626368B1 (de) 2004-07-19 2016-12-21 Biocon Limited Insulinoligomerkonjugate, Formulierungen und Verwendungen davon
US7935788B2 (en) * 2004-10-04 2011-05-03 National Research Council Of Canada Reverse cumate repressor mutant
EP1797186B1 (de) * 2004-10-04 2016-05-25 National Research Council Of Canada Expressionssystem, dessen komponenten und verwendungsverfahren
WO2009032664A2 (en) * 2007-08-31 2009-03-12 The Regents Of The University Of Michigan Methods and compositions for increasing biological molecule stability
RU2453332C2 (ru) 2007-10-16 2012-06-20 Байокон Лимитид Твердая фармацевтическая композиция (варианты) и способ контроля концентрации глюкозы с ее помощью, способ получения твердой фармацевтической композиции (варианты), таблетка (варианты) и способ получения амфорных частиц
EP2552935B1 (de) * 2010-03-31 2020-05-27 AMSilk GmbH Auftrennung von unlöslichen proteinen
RU2497118C1 (ru) * 2012-03-06 2013-10-27 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) БЫСТРЫЙ СПОСОБ ОБРАБОТКИ ГЛАДКОМЫШЕЧНЫХ КЛЕТОК КИШЕЧНИКА ГЕМОЛИЗИНОМ II Bacillus cereus
CN102617703A (zh) * 2012-04-14 2012-08-01 拜明(苏州)生物技术有限公司 一种无需裂解高效提取发酵细菌中外源表达蛋白的方法
WO2015176067A2 (en) 2014-05-16 2015-11-19 The Regents Of The University Of California A long non-coding rna expressed in aggressive cancer
US10745457B2 (en) 2015-09-02 2020-08-18 Merck Sharp & Dohme Corp. Process for obtaining insulin with correctly formed disulfide bonds

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
US4624926A (en) * 1981-01-02 1986-11-25 The Research Foundation Of State University Of New York Novel cloning vehicles for polypeptide expression in microbial hosts
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US4503155A (en) * 1982-02-01 1985-03-05 Eli Lilly And Company Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli
US4732859A (en) * 1982-09-03 1988-03-22 Eli Lilly And Company Method for conferring bacteriophage resistance to bacteria
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4578355A (en) * 1983-01-12 1986-03-25 The United States Of America As Represented By The Department Of Health And Human Services Plasmid cloning vector pAS1
US5256546A (en) * 1983-07-15 1993-10-26 Bio-Technology General Corp. Bacterial expression of porcine growth hormone
US4734368A (en) * 1984-03-23 1988-03-29 Huels Aktiengesellschaft Process for the bioconversion of fumarate to L-malate
US4870013A (en) * 1984-04-19 1989-09-26 Cetus Corporation SV40 early and RSV promoters useful in saccharomyces expression
US4581170A (en) * 1984-08-03 1986-04-08 E. R. Squibb & Sons, Inc. N-hydroxyl protecting groups and process and intermediates for the preparation of 3-acylamino-1-hydroxy-2-azetidinones
US5032510A (en) * 1984-09-26 1991-07-16 Eli Lilly And Company Method for expression and secretion in bacillus
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
CA1304886C (en) * 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
CA1339955C (en) * 1986-10-14 1998-07-14 Richard Eugene Heiney Process for transforming a human insulin precursor to human insulin
SU1703693A1 (ru) * 1987-02-09 1992-01-07 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Рекомбинантна плазмидна ДНК @ - @ 2-19, кодирующа синтез интерлейкина-2 человека, способ ее конструировани @ штамм бактерий ЕSснеRIснIа coLI - продуцент интерлейкина-2 человека
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US5063158A (en) * 1987-11-09 1991-11-05 Eli Lilly And Company Recombinant DNA expression vector comprising both transcriptional and translational activating sequences
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5026639A (en) * 1988-01-14 1991-06-25 Nippon Mining Company, Limited Method to improve mRNA translation and use thereof for production of platelet factor-4
US5258287A (en) * 1988-03-22 1993-11-02 Genentech, Inc. DNA encoding and methods of production of insulin-like growth factor binding protein BP53
EP0337243A1 (de) * 1988-04-14 1989-10-18 F. Hoffmann-La Roche Ag Verfahren zur Reinigung von rekombinantem Interleukin-2
US5262322A (en) * 1988-06-24 1993-11-16 Genentech, Inc. Host transformed with yeast gene and ubiquitin/polypeptide fusions
US4897465A (en) * 1988-10-12 1990-01-30 Abbott Laboratories Enrichment and concentration of proteins by ultrafiltration
US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
WO1991011454A1 (en) * 1990-01-24 1991-08-08 The Upjohn Company Method of purifying recombinant polypeptides
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US5460954A (en) * 1992-04-01 1995-10-24 Cheil Foods & Chemicals, Inc. Production of human proinsulin using a novel vector system
US5648235A (en) * 1992-07-03 1997-07-15 Q.B.I. Enterprises Ltd. Method and means for the production of gene products, novel recombinant DNA vectors therefor and kits employing them
GB9224584D0 (en) * 1992-11-23 1993-01-13 Connaught Lab Use of outer membrane protein d15 and its peptides as vaccine against haempohilus influenzae diseases
US5648244A (en) * 1993-09-27 1997-07-15 President And Fellows Of Harvard College Production, purification, cleavage and use of fusion peptides
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
US5534428A (en) * 1994-09-06 1996-07-09 Life Technologies, Inc. Cloned Ssti/SacI restriction-modification system
US5850015A (en) * 1995-06-07 1998-12-15 Cornell Research Foundation, Inc. Hypersensitive response elicitor from Erwinia chrysanthemi
ZA979496B (en) * 1996-10-31 1999-04-23 Genentech Inc Assay using marked microbial host cell strains

Also Published As

Publication number Publication date
IL133832A (en) 2007-07-04
US6281329B1 (en) 2001-08-28
BR9810650A (pt) 2002-07-23
IN187721B (de) 2002-06-15
US6699692B2 (en) 2004-03-02
US6509452B1 (en) 2003-01-21
BR9810650B1 (pt) 2010-12-14
EP0996713B1 (de) 2004-10-06
US20030082742A1 (en) 2003-05-01
PL348669A1 (en) 2002-06-03
IL133832A0 (en) 2001-04-30
RU2222598C2 (ru) 2004-01-27
CA2687542C (en) 2013-04-30
PL194141B1 (pl) 2007-04-30
US6068993A (en) 2000-05-30
ATE278776T1 (de) 2004-10-15
WO1999001548A2 (en) 1999-01-14
CA2687542A1 (en) 1999-01-14
WO1999001548A3 (en) 1999-09-10
CA2294760A1 (en) 1999-01-14
CA2294760C (en) 2010-02-23
EP0996713A1 (de) 2000-05-03
DE69826864T2 (de) 2005-11-03

Similar Documents

Publication Publication Date Title
Laban et al. Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.
DE69826864D1 (de) Vektor zur heterologen proteinexpression und verfahren zur extraktion rekombinanter proteine sowie zur isolierung rekombinanten insulins
Lewis et al. Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein
Ikeda et al. Human transforming growth factor type. beta. 2: production by a prostatic adenocarcinoma cell line, purification, and initial characterization
Wagner et al. A high-mobility-group protein and its cDNAs from Drosophila melanogaster
KR880014110A (ko) 인체 프로아포지방단백질 a-i의 발현
CN107532190A (zh) 用于肽生产的融合伴侣
WO1999020766A3 (en) NOVEL MODIFIED NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING mRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS
WO1994018332A3 (en) Anthrax toxin fusion proteins and uses thereof
WO2000009734A3 (en) Methods and compositions for use in spliceosome mediated rna trans-splicing
AU1276295A (en) Expression of self-processing polyproteins in transgenic plants
KR970701199A (ko) 성장 정지 호메오박스 유전자(Growth Arrest Homeobox Gene)
WO1998055606A3 (en) Lactoferrin receptor genes of moraxella
DE69735264D1 (de) Gefrierschutzproteine aus choristoneura sp., gene und verfahren zu ihrer verwendung
KR960701096A (ko) 그룹 B 스트렙토코커스의 많은 균주들에 대한 면역성을 제공하는 세포 표면 단백질인 Rib 단백질, 그 단백질의 정제방법, 시약키트 및 제약조성물(PROTEIN RIB. A CELL SURFACE PROTEIN THAT CONFERS IMMUNITY TO MANY STRAINS OF THE GROUP B STREPTOCOCCUS; PROCESS FOR PURIFICATION OF THE PROTEIN, PEAGENT KIT AND PHARMACEUTICAL COMPOSITION)
ATE286069T1 (de) Verfahren zur herstellung rekombinanter pilc- proteine
Roberts et al. Synthesis and posttranslational segregation of glyoxysomal isocitrate lyase from castor bean endosperm
NO930428L (no) Proteinstrukturen til plantetoksingelonin
Rosenberg et al. T7 RNA polymerase can direct expression of influenza virus cap-binding protein (PB2) in Escherichia coli
WO1998027200A3 (en) Human and mammalian dna replication origin consensus sequences
KR850001286A (ko) 개량 플라스미드 벡타의 제조방법
WO1992007868A1 (en) A method for isolating and purifying peptides and proteins
AU5076393A (en) Proteins which regulate the expression of vertebrate MHC class II genes, DNA sequences encoding them and pharmaceutical compositions
WO2009054754A1 (fr) Plasmide recombinant phins21 codant une protéine hybride avec la proinsuline humaine, souche de bactéries escherichia coli jm109/ phins21 productrice de la protéine hybride avec la proinsuline humaine et procédé de fabrication de proinsuline humaine
AU2001253258A1 (en) Human trp-like calcium channel protein-2 (tlcc-2)

Legal Events

Date Code Title Description
8364 No opposition during term of opposition