RU2014139000A - Энхансер палочковидного вируса сахарного тростника (scbv) и его применение в функциональной геномике растений - Google Patents
Энхансер палочковидного вируса сахарного тростника (scbv) и его применение в функциональной геномике растений Download PDFInfo
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- 108020004999 messenger RNA Proteins 0.000 claims abstract 2
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- 230000009261 transgenic effect Effects 0.000 claims 10
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Abstract
1. Химерная область регуляции транскрипции, содержащая:одну или несколько копий энхансерного элемента палочковидного вируса сахарного тростника (SCBV), представленного от положения 337 до положения 618 в SEQ ID NO: 1, или его гомолога; ифункционально связанный с ним промотор, получаемый из области, расположенной выше специфичного для семян гена, где промотор содержит участок связывания РНК-полимеразы и участок инициации иРНК,где, когда представляющая интерес нуклеотидная последовательность транскрибируется под регуляторным контролем химерной области регуляции транскрипции, количество продукта транскрипции по сравнению с количеством продукта транскрипции, получаемым с химерной областью регуляции транскрипции, содержащей промотор и не содержащей энхансерной последовательности(ей) SCBV, увеличивается.2. Химерная область регуляции транскрипции по п. 1, где промотор получают из области, расположенной выше специфичного для семян гена 3-кетоацил-КоА Lesquerella fendleri.3. Химерная область регуляции транскрипции по п. 1, где нуклеотидная последовательность представляет собой нуклеотидную последовательность модификации жирных кислот.4. Химерная область регуляции транскрипции по п. 3, где нуклеотидная последовательность модификации жирных кислот состоит из нуклеотидной последовательности ацил-КоА-дельта-9-десатуразы Aspergillus nidulans.5. Конструкция, содержащая химерную область регуляции транскрипции по п. 1, функционально связанную с транскрибируемой полинуклеотидной молекулой, функционально связанной с 3'-концевой полинуклеотидной молекулой терминации транскрипции.6. Конструкция по п. 5, где указанная транскрибируемая полинуклеотидная молекула придает растению, в котором о
Claims (27)
1. Химерная область регуляции транскрипции, содержащая:
одну или несколько копий энхансерного элемента палочковидного вируса сахарного тростника (SCBV), представленного от положения 337 до положения 618 в SEQ ID NO: 1, или его гомолога; и
функционально связанный с ним промотор, получаемый из области, расположенной выше специфичного для семян гена, где промотор содержит участок связывания РНК-полимеразы и участок инициации иРНК,
где, когда представляющая интерес нуклеотидная последовательность транскрибируется под регуляторным контролем химерной области регуляции транскрипции, количество продукта транскрипции по сравнению с количеством продукта транскрипции, получаемым с химерной областью регуляции транскрипции, содержащей промотор и не содержащей энхансерной последовательности(ей) SCBV, увеличивается.
2. Химерная область регуляции транскрипции по п. 1, где промотор получают из области, расположенной выше специфичного для семян гена 3-кетоацил-КоА Lesquerella fendleri.
3. Химерная область регуляции транскрипции по п. 1, где нуклеотидная последовательность представляет собой нуклеотидную последовательность модификации жирных кислот.
4. Химерная область регуляции транскрипции по п. 3, где нуклеотидная последовательность модификации жирных кислот состоит из нуклеотидной последовательности ацил-КоА-дельта-9-
десатуразы Aspergillus nidulans.
5. Конструкция, содержащая химерную область регуляции транскрипции по п. 1, функционально связанную с транскрибируемой полинуклеотидной молекулой, функционально связанной с 3'-концевой полинуклеотидной молекулой терминации транскрипции.
6. Конструкция по п. 5, где указанная транскрибируемая полинуклеотидная молекула придает растению, в котором она экспрессируется, агрономический признак.
7. Конструкция по п. 5, где указанная транскрибируемая полинуклеотидная молекула придает растению, в котором она экспрессируется, признак добавления значения модификации масел.
8. Трансгенное растение, стабильно трансформированное конструкцией по п. 5.
9. Трансгенное растение по п. 8, где транскрибируемая полинуклеотидная молекула придает растению, в котором она экспрессируется, агрономический признак.
10. Трансгенное растение по п. 8, где транскрибируемая полинуклеотидная молекула придает растению признак добавления значения или модификации масел.
11. Семя трансгенного растения по п. 8, где семя содержат конструкцию по п. 5.
12. Трансгенное растение по п. 8, где растение представляет собой растение Arabidopsis thaliana.
13. Клетка трансгенного растения, содержащая химерную область регуляции транскрипции по п. 1.
14. Способ получения трансгенного растения, включающий трансформацию клетки или ткани растения конструкцией по п. 5.
15. Способ по п. 14, где трансгенное растение является двудольным.
16. Способ по п. 14, где трансгенное растение является однодольным.
17. Клетка или ткань растения, трансформированные конструкцией по п. 5.
18. Клетка или ткань растения по п. 17, где клетка или ткань растения происходят из двудольного растения.
19. Клетка или ткань растения по п. 17, где клетка или ткань растения происходят из однодольного растения.
20. Клетка, плод, лист, корень, побег, цветок, семя, срез и другие репродуктивные материалы растения, пригодные для полового или бесполого размножения, потомство растения, включая гибриды F1, стерильные по мужскому полу растения и все другие растения и растительные продукты, получаемые из трансгенного растения по п. 8.
21. Клетка, ткань растения Arabidopsis thaliana или растение Arabidopsis thaliana, содержащие четыре копии энхансерного элемента палочковидного вируса сахарного тростника (SCBV) от положения 337 до положения 618 SEQ ID NO: 1 или его гомолога, где четыре копии энхансерного элемента SCBV встроены в геном клетки, ткани растения Arabidopsis thaliana или растения Arabidopsis thaliana.
22. Клетка, ткань растения Arabidopsis thaliana или растение Arabidopsis thaliana по п. 21, где энхансерные элементы SCBV обеспечивают усиленную транскрипцию представляющей интерес нуклеотидной последовательности, которая находится под
регуляторным контролем энхансера SCBV, по сравнению с транскрипцией представляющей интерес нуклеотидной последовательности в отсутствие энхансера SCBV.
23. Способ усиления транскрипции представляющей интерес нуклеотидной последовательности, включающий:
трансформацию конструкции ДНК, содержащей SEQ ID NO: 2, в геном растения Arabidopsis thaliana и
экспрессию конструкции ДНК в семенах растения, что приводит к модифицированному профилю жирных кислот в семенах.
24. Способ по п. 23, где модифицированный профиль жирных кислот содержит сниженный процент насыщенных жирных кислот.
25. Способ по п. 23, где конструкция ДНК содержит четыре копии энхансерного элемента SCBV, промотор, получаемый из области, расположенной выше специфичного для семян гена 3-кетоацил-КоА Lesquerella fendleri, нуклеотидную последовательность ацил-КоА-дельта-9-десатуразы Aspergillus nidulans и 3'-нетранслируемую область открытой рамки считывания 23 Agrobacterium tumefaciens.
26. Способ по п. 23, где трансформация включает опосредованное Agrobacterium tumefaciens погружение цветов.
27. Способ по п. 23, где экспрессия включает специфичную для семян экспрессию.
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| US201261605147P | 2012-02-29 | 2012-02-29 | |
| US61/605,147 | 2012-02-29 | ||
| PCT/US2013/028331 WO2013130813A1 (en) | 2012-02-29 | 2013-02-28 | Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics |
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| AU2013202941B2 (en) | 2012-02-29 | 2015-06-25 | Dow Agrosciences Llc | Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics |
| CN105705007A (zh) | 2013-09-13 | 2016-06-22 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
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| CA2977026A1 (en) | 2015-03-11 | 2016-09-15 | E.I. Du Pont De Nemours And Company | Insecticidal combinations of pip-72 and methods of use |
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| ATE71981T1 (de) | 1984-12-10 | 1992-02-15 | Monsanto Co | Einsetzung des bacillus thuringiensis kristallproteingens in pflanzen kolonisierende mikroorganismen und deren verwendung. |
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| CN104411827B (zh) | 2018-01-02 |
| JP2015508668A (ja) | 2015-03-23 |
| EP2820136B1 (en) | 2018-03-28 |
| EP2820136A1 (en) | 2015-01-07 |
| WO2013130813A1 (en) | 2013-09-06 |
| AU2013202941A1 (en) | 2013-09-19 |
| BR112014021426A2 (pt) | 2017-07-18 |
| CA2865977A1 (en) | 2013-09-06 |
| US20150059021A1 (en) | 2015-02-26 |
| KR20140130506A (ko) | 2014-11-10 |
| AR090204A1 (es) | 2014-10-29 |
| AU2013202941B2 (en) | 2015-06-25 |
| IN2014DN06986A (ru) | 2015-04-10 |
| CN104411827A (zh) | 2015-03-11 |
| RU2639517C2 (ru) | 2017-12-21 |
| US10077450B2 (en) | 2018-09-18 |
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