RU2002116217A - Электрохимический способ и устройства, предназначенные для применения при определении концентраций исследуемых веществ с поправкой на гематокритное число - Google Patents

Электрохимический способ и устройства, предназначенные для применения при определении концентраций исследуемых веществ с поправкой на гематокритное число

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Publication number
RU2002116217A
RU2002116217A RU2002116217/15A RU2002116217A RU2002116217A RU 2002116217 A RU2002116217 A RU 2002116217A RU 2002116217/15 A RU2002116217/15 A RU 2002116217/15A RU 2002116217 A RU2002116217 A RU 2002116217A RU 2002116217 A RU2002116217 A RU 2002116217A
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Prior art keywords
polarity
time
concentration
hematocrit
current
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RU2002116217/15A
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RU2262890C2 (ru
Inventor
Тимоти Дж. ОХАРА (US)
Тимоти Дж. ОХАРА
Махиар З. КЕРМАНИ (US)
Махиар З. КЕРМАНИ
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Лайфскен, Инк. (Us)
Лайфскен, Инк.
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (10)

1. Способ определения концентрации, исследуемого вещества в физиологической пробе с учетом поправки на гематокритное число, заключающийся в том, что (а) вводят упомянутую физиологическую пробу в электрохимическую ячейку, содержащую (i) отстоящие друг от друга рабочий электрод и электрод сравнения и (ii) систему окислительно-восстановительных реактивов, содержащую фермент и медиатор, (б) прикладывают первый электрический потенциал, имеющий первую полярность, к упомянутой реакционной ячейке и измеряют ток в ячейке как функцию времени для получения первой времятоковой переходной характеристики, и (в) прикладывают второй электрический потенциал, имеющий вторую полярность, к упомянутой ячейке, причем вторая полярность противоположна упомянутой первой полярности, и измеряют ток в ячейке как функцию времени для получения второй времятоковой переходной характеристики, и (г) получают предварительную концентрацию исследуемого вещества на основании упомянутых первой и второй времятоковых переходных характеристик, и (д) умножают упомянутую предварительную концентрацию исследуемого вещества за вычетом значения фона на коэффициент поправки на гематокритное число для получения упомянутой концентрации, упомянутого исследуемого вещества в упомянутой пробе с учетом поправки на гематокритное число, посредством чего определяют концентрацию, в которой учтена поправка на гематокритное число, упомянутого исследуемого вещества в упомянутой пробе.
2. Способ по п.1, отличающийся тем, что упомянутый коэффициент поправки на гематокритное число модифицирует значение предварительной концентрации исследуемого вещества для удаления составляющей, обусловленной гематокритным числом, из упомянутого значения предварительной концентрации исследуемого вещества.
3. Способ по п.1 или 2, отличающийся тем, что упомянутый коэффициент поправки на гематокритное число является функцией упомянутой предварительной концентрации исследуемого вещества и переменной γ упомянутой электрохимической ячейки.
4. Способ по пп.1, 2 или 3, отличающийся тем, что упомянутой физиологической пробой является цельная кровь или ее производное.
5. Способ по п.4, отличающийся тем, что упомянутым исследуемым веществом является глюкоза.
6. Способ по п.5, отличающийся тем, что упомянутый коэффициент поправки на гематокритное число равен
1/((0,6637)+((4,9466*1n(С0))/С0)+(-0,4012*1n(γ))),
где С0 - упомянутая предварительная концентрация;
γ - упомянутое переменное соотношение токов упомянутой электрохимической ячейки.
7. Способ по любому из пп.1-16, отличающийся тем, что упомянутая первая полярность является отрицательной, а упомянутая вторая полярность является положительной.
8. Измерительное устройство, предназначенное для амперометрического измерения концентрации исследуемого вещества в физиологической пробе согласно способу по любому из пп.1-7, содержащее (а) средство для приложения первого электрического потенциала, имеющего первую электрическую полярность, к электрохимической ячейке, содержащей упомянутую пробу, и измерения тока в этой ячейке как функции времени для получения первой времятоковой переходной характеристики, (б) средство для приложения второго электрического потенциала, имеющего вторую полярность, к упомянутой электрохимической ячейке, причем вторая полярность противоположна упомянутой первой полярности, и измерения тока в этой ячейке как функции времени для получения второй времятоковой переходной характеристики, (в) средство для определения значения предварительной концентрации исследуемого вещества и переменной γ на основании упомянутых первой и второй времятоковых переходных характеристик, и (г) средство для удаления составляющей, обусловленной гематокритным числом, из упомянутого значения предварительной концентрации с целью получения упомянутой концентрации исследуемого вещества в упомянутой пробе.
9. Измерительное устройство по п.8, отличающееся тем, что включает в себя электрохимическую индикаторную полоску.
10. Система, предназначенная для применения при определении концентрации исследуемого вещества в физиологической пробе любым из способов по пп.1-7, содержащая (а) электрохимическую индикаторную полоску и (б) измерительное устройство, содержащее средство для приложения первого электрического потенциала, имеющего первую электрическую полярность, к электрохимической ячейке, содержащей упомянутую пробу, и измерения тока в этой ячейке как функции времени для получения первой времятоковой переходной характеристики, (2) средство для приложения второго электрического потенциала, имеющего вторую полярность, к упомянутой электрохимической ячейке, причем вторая полярность противоположна упомянутой первой полярности, и измерения тока в этой ячейке как функции времени для получения второй времятоковой переходной характеристики, (3) средство для определения значения предварительной концентрации исследуемого вещества и переменной γ на основании упомянутых первой и второй времятоковых переходных характеристик, и (4) средство для удаления составляющей, обусловленной гематокритным числом, из упомянутого значения предварительной концентрации с целью получения упомянутой концентрации исследуемого вещества в упомянутой пробе.
RU2002116217/14A 2000-02-02 2001-01-25 Электрохимический способ и устройства, предназначенные для применения при определении концентраций исследуемых веществ с поправкой на гематокритное число RU2262890C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/497,304 US6475372B1 (en) 2000-02-02 2000-02-02 Electrochemical methods and devices for use in the determination of hematocrit corrected analyte concentrations
US09/497,304 2000-02-02

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RU2002116217A true RU2002116217A (ru) 2004-01-20
RU2262890C2 RU2262890C2 (ru) 2005-10-27

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RU2005123794/28A RU2005123794A (ru) 2000-02-02 2005-07-26 Способ определения концентрации исследуемого вещества в физиологической пробе

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US (3) US6475372B1 (ru)
EP (2) EP1645876A3 (ru)
JP (1) JP2003521708A (ru)
KR (1) KR20020077409A (ru)
CN (1) CN1193228C (ru)
AR (1) AR027345A1 (ru)
AT (1) ATE313794T1 (ru)
AU (1) AU783311B2 (ru)
CA (1) CA2398203A1 (ru)
CZ (1) CZ20021298A3 (ru)
DE (1) DE60116056T2 (ru)
DK (1) DK1252514T3 (ru)
ES (1) ES2254368T3 (ru)
HK (1) HK1049881B (ru)
IL (1) IL149662A (ru)
MX (1) MXPA02005797A (ru)
MY (1) MY127335A (ru)
PL (1) PL358492A1 (ru)
RU (2) RU2262890C2 (ru)
TW (1) TW520439B (ru)
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US20050176153A1 (en) 2005-08-11
EP1645876A2 (en) 2006-04-12
JP2003521708A (ja) 2003-07-15
RU2005123794A (ru) 2007-02-10
TW520439B (en) 2003-02-11
ATE313794T1 (de) 2006-01-15
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DK1252514T3 (da) 2006-05-01
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DE60116056T2 (de) 2006-08-10
IL149662A (en) 2005-07-25
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US20020125145A1 (en) 2002-09-12
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ES2254368T3 (es) 2006-06-16
CN1394280A (zh) 2003-01-29
AU783311B2 (en) 2005-10-13
US6890421B2 (en) 2005-05-10
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WO2001057510A3 (en) 2002-02-21
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