RU2002105508A - Мутантные ферменты Тn5-транспозазы и способ их применения - Google Patents

Мутантные ферменты Тn5-транспозазы и способ их применения

Info

Publication number
RU2002105508A
RU2002105508A RU2002105508/13A RU2002105508A RU2002105508A RU 2002105508 A RU2002105508 A RU 2002105508A RU 2002105508/13 A RU2002105508/13 A RU 2002105508/13A RU 2002105508 A RU2002105508 A RU 2002105508A RU 2002105508 A RU2002105508 A RU 2002105508A
Authority
RU
Russia
Prior art keywords
transposase
amino acid
mutant
acid sequence
sequence
Prior art date
Application number
RU2002105508/13A
Other languages
English (en)
Russian (ru)
Inventor
Уилль м С. РЕЗНИКОФФ (US)
Уилльям С. РЕЗНИКОФФ
Тодд А. НОМАНН (US)
Тодд А. НОМАНН
Original Assignee
Висконсин Элумни Рисёч Фаундейшн (Us)
Висконсин Элумни Рисёч Фаундейшн
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Висконсин Элумни Рисёч Фаундейшн (Us), Висконсин Элумни Рисёч Фаундейшн filed Critical Висконсин Элумни Рисёч Фаундейшн (Us)
Publication of RU2002105508A publication Critical patent/RU2002105508A/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
RU2002105508/13A 1999-08-02 2000-08-02 Мутантные ферменты Тn5-транспозазы и способ их применения RU2002105508A (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14668699P 1999-08-02 1999-08-02
US60/146,686 1999-08-02

Publications (1)

Publication Number Publication Date
RU2002105508A true RU2002105508A (ru) 2004-01-27

Family

ID=22518527

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2002105508/13A RU2002105508A (ru) 1999-08-02 2000-08-02 Мутантные ферменты Тn5-транспозазы и способ их применения

Country Status (8)

Country Link
EP (1) EP1198583A1 (ja)
JP (1) JP2003505104A (ja)
CN (1) CN1367840A (ja)
AU (1) AU775043B2 (ja)
CA (1) CA2380850A1 (ja)
PL (1) PL353241A1 (ja)
RU (1) RU2002105508A (ja)
WO (1) WO2001009363A1 (ja)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
US20020072097A1 (en) * 2000-07-07 2002-06-13 Delcardayre Stephen Molecular breeding of transposable elements
CA2431246A1 (en) 2000-12-05 2002-06-13 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
CN101698848B (zh) * 2009-08-26 2012-10-03 上海海洋大学 金鱼自主转座子基因gfTol2、该基因编码的转座酶及该基因的用途
PT2556171E (pt) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Ensaios biológicos codificados espacialmente
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
DK3013984T3 (da) 2013-06-25 2023-06-06 Prognosys Biosciences Inc Metode til bestemmelse af spatiale mønstre i biologiske targets i en prøve
CN103602654B (zh) * 2013-11-20 2016-02-24 浙江农林大学 人工改造的高活性Mariner-Like转座酶
US9790476B2 (en) * 2014-04-15 2017-10-17 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased DNA input tolerance
FI3901281T3 (fi) 2015-04-10 2023-01-31 Biologisten näytteiden spatiaalisesti eroteltu moninkertainen nukleiinihappoanalyysi
CN106754811B (zh) * 2016-12-21 2019-05-14 南京诺唯赞生物科技有限公司 一种突变型Tn5转座酶及其制备方法和应用
ES2933806T3 (es) 2017-02-21 2023-02-14 Illumina Inc Tagmentación que usa transposomas inmovilizados con enlazadores
CN108265040B (zh) * 2017-09-24 2021-02-09 天津强微特生物科技有限公司 一种嗜盐古细菌中的转座酶高活性突变体
EP3844304B1 (en) 2018-08-28 2024-10-02 10X Genomics, Inc. Methods for generating spatially barcoded arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3887540B1 (en) 2018-11-30 2023-08-23 Illumina, Inc. Analysis of multiple analytes using a single assay
EP3894587A1 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Resolving spatial arrays by proximity-based deconvolution
BR112021006095A2 (pt) 2018-12-17 2021-07-20 Illumina, Inc. métodos e meios para preparação de uma biblioteca para sequenciamento
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
JP2022539630A (ja) 2019-07-12 2022-09-13 イルミナ ケンブリッジ リミテッド 電気泳動を用いた核酸ライブラリの調製
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4424843A3 (en) 2019-12-23 2024-09-25 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115916999A (zh) 2020-04-22 2023-04-04 10X基因组学有限公司 用于使用靶向rna耗竭进行空间分析的方法
EP4414459A3 (en) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
EP4450639A2 (en) 2020-06-25 2024-10-23 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
EP4347879A1 (en) 2021-06-03 2024-04-10 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof

Also Published As

Publication number Publication date
AU775043B2 (en) 2004-07-15
PL353241A1 (en) 2003-11-03
JP2003505104A (ja) 2003-02-12
EP1198583A1 (en) 2002-04-24
CN1367840A (zh) 2002-09-04
CA2380850A1 (en) 2001-02-08
WO2001009363A1 (en) 2001-02-08
AU6396700A (en) 2001-02-19

Similar Documents

Publication Publication Date Title
RU2002105508A (ru) Мутантные ферменты Тn5-транспозазы и способ их применения
US8188260B2 (en) Versatile acylation catalytic RNAs and uses thereof
RU99107555A (ru) Система для транспозиции in vitro, на основе использовании модифицированной tn5-транспозазы
US20050266439A1 (en) Thermostable RNA ligase from thermus phage
CA2378871A1 (en) Design of beta-sheet proteins with specific binding properties
JP7342040B6 (ja) Ａｄｈタンパク質ファミリー変異体およびその使用
JP2020518278A (ja) 遺伝子操作されたリガーゼバリアント
JP2001505403A (ja) エステラーゼ
CN107614689A (zh) 用于将非天然氨基酸并入蛋白质中的平台
CN110408635B (zh) 一种含有链霉亲和素元件的核酸构建物在蛋白质表达、纯化中的应用
Dreßen et al. Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2): A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids: Part I: Comparative characterization to the enzymes from Petroselinum crispum (PcPAL1) and Rhodosporidium toruloides (RtPAL)
CA2590245A1 (en) Ladder assembly and system for generating diversity
Menick et al. Cys154 is important for lac permease activity in Escherichia coli
CA2400049A1 (en) Rna polymerase mutants with increased thermostability
JP6967002B2 (ja) トランスグルタミナーゼ認識部位を有するｆｋｂｐドメイン
EP4368708A1 (en) Nucleic acid ligase
JP4752026B2 (ja) 新規なピリドキサール４−デヒドロゲナーゼ及びその利用
JP2001017172A (ja) 二本鎖ｄｎａ断片の末端での連結
US20220002719A1 (en) Oligonucleotide-mediated sense codon reassignment
Cheok et al. Phage based screening strategy for identifying enzyme substrates
CN111378706A (zh) 通过Edc3基因敲除改变体外蛋白合成能力的方法及其应用
ES2966310T3 (es) Biocatalizador como componente de núcleo de un sistema redox catalizado por enzima para la reducción biocatalítica de cistina
WO2024141087A1 (zh) 一种用于插入非天然氨基酸的体外无细胞蛋白质合成体系、方法及应用
JP6804467B2 (ja) 組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法
Hayes et al. Crosslinking of base modified RNAs by synthetic DYW-KP base editors implicates an enzymatic lysine as the nitrogen donor for U-to-C RNA editing

Legal Events

Date Code Title Description
FA92 Acknowledgement of application withdrawn (lack of supplementary materials submitted)

Effective date: 20050408