PT2102331E - Vacina antitumoral derivada de células cd4+ normais modificadas quimicamente - Google Patents
Vacina antitumoral derivada de células cd4+ normais modificadas quimicamente Download PDFInfo
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- PT2102331E PT2102331E PT08701232T PT08701232T PT2102331E PT 2102331 E PT2102331 E PT 2102331E PT 08701232 T PT08701232 T PT 08701232T PT 08701232 T PT08701232 T PT 08701232T PT 2102331 E PT2102331 E PT 2102331E
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| US8666674B2 (en) | 2009-07-28 | 2014-03-04 | Promising Future, Llc | Pairing processes for preparing reactive cytotoxic T cells |
| US8467973B2 (en) | 2009-07-28 | 2013-06-18 | Promising Future, Llc | Pairing processes for preparing alloreactive cytotoxic T cells |
| EP2494038B1 (en) | 2009-10-27 | 2019-06-26 | Immunicum AB | Method for proliferation of antigen-specific t cells |
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| US10426740B1 (en) | 2010-08-18 | 2019-10-01 | Avm Biotechnology, Llc | Compositions and methods to inhibit stem cell and progenitor cell binding to lymphoid tissue and for regenerating germinal centers in lymphatic tissues |
| KR102349677B1 (ko) | 2015-05-28 | 2022-01-12 | 카이트 파마 인코포레이티드 | T 세포 요법을 위해 환자를 컨디셔닝하는 방법 |
| CA2986793A1 (en) | 2015-05-28 | 2016-12-01 | Kite Pharma, Inc. | Diagnostic methods for t cell therapy |
| KR20180063320A (ko) | 2015-10-20 | 2018-06-11 | 카이트 파마 인코포레이티드 | T 세포 요법을 위한 t 세포를 제조하는 방법 |
| CA3019650C (en) | 2016-04-01 | 2023-07-25 | Kite Pharma, Inc. | Chimeric receptors and methods of use thereof |
| TWI795133B (zh) | 2016-04-01 | 2023-03-01 | 美商凱特製藥公司 | Ｂｃｍａ結合分子類及彼等之用途 |
| ES2891578T3 (es) | 2016-04-01 | 2022-01-28 | Kite Pharma Inc | Antígeno quimérico y receptores de células T y métodos de uso |
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| EP3490605B1 (en) | 2017-04-01 | 2023-06-07 | AVM Biotechnology, LLC | Replacement of cytotoxic preconditioning before cellular immunotherapy |
| SG11201908980PA (en) | 2017-04-03 | 2019-10-30 | Kite Pharma Inc | Treatment using chimeric receptor t cells incorporating optimized polyfunctional t cells |
| AU2018257894A1 (en) | 2017-04-24 | 2019-10-31 | Kite Pharma, Inc. | Humanized antigen-binding domains against CD19 and methods of use |
| WO2018218105A1 (en) | 2017-05-26 | 2018-11-29 | Kite Pharma, Inc. | Methods of making and using embryonic mesenchymal progenitor cells |
| CA3075619A1 (en) | 2017-09-15 | 2019-03-21 | Kite Pharma, Inc. | Methods and systems for performing a patient-specific immunotherapy procedure with chain-of-custody and chain-of-identity biological sample tracking |
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| MX421741B (es) | 2017-10-18 | 2025-03-14 | Kite Pharma Inc | Inmunoterapia con receptores de antígeno quimérico y uso de la misma |
| EP3710471A1 (en) | 2017-11-16 | 2020-09-23 | Kite Pharma, Inc. | Modified chimeric antigen receptors and methods of use |
| CA3087481A1 (en) | 2018-01-15 | 2019-07-18 | Pfizer Inc. | Methods of administering chimeric antigen receptor immunotherapy in combination with 4-1bb agonist |
| WO2019152957A1 (en) | 2018-02-02 | 2019-08-08 | Arizona Board Of Regents On Behalf Of Arizona State University | Dna-chimeric antigen receptor t cells for immunotherapy |
| CA3177757A1 (en) | 2018-02-16 | 2019-08-22 | Kite Pharma, Inc. | Modified pluripotent stem cells and methods of making and use |
| TW202322828A (zh) | 2018-04-12 | 2023-06-16 | 美商凱特製藥公司 | 利用腫瘤微環境之特性之嵌合受體ｔ細胞治療 |
| JP7174777B2 (ja) | 2018-06-01 | 2022-11-17 | カイト ファーマ インコーポレイテッド | キメラ抗原受容体ｔ細胞療法 |
| US20210155941A1 (en) | 2018-06-22 | 2021-05-27 | Kite Pharma Eu B.V. | Compositions and methods for making engineered t cells |
| IL311860A (en) | 2018-08-02 | 2024-06-01 | Kite Pharma Inc | Treatment with receptor-antigen chimeric kinetics of T cell expansion and their uses |
| EP3488851A1 (en) | 2018-10-03 | 2019-05-29 | AVM Biotechnology, LLC | Immunoablative therapies |
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| AU2019397033B2 (en) | 2018-12-12 | 2023-06-29 | Kite Pharma, Inc. | Chimeric antigen receptors and CAR-T cells and methods of use |
| US20220175900A1 (en) * | 2019-04-12 | 2022-06-09 | Cytovac A/S | Method for preparation of cancer/testis antigen-specific t-cells |
| CN118593691A (zh) | 2019-05-03 | 2024-09-06 | 凯德药业股份有限公司 | 施用嵌合抗原受体免疫疗法的方法 |
| CN114391021B (zh) | 2019-06-21 | 2026-01-16 | 凯德药业股份有限公司 | TGF-β受体和使用方法 |
| CA3147441A1 (en) | 2019-09-03 | 2021-03-11 | Allogene Therapeutics, Inc. | Methods of preparing t cells for t cell therapy |
| IL292665B2 (en) | 2019-11-06 | 2026-02-01 | Kite Pharma Inc | Chimeric antigen receptor T cell therapy |
| US20230293530A1 (en) | 2020-06-24 | 2023-09-21 | Yeda Research And Development Co. Ltd. | Agents for sensitizing solid tumors to treatment |
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| TW202241469A (zh) | 2021-02-20 | 2022-11-01 | 美商凱特製藥公司 | 免疫療法 |
| EP4305053A1 (en) | 2021-03-11 | 2024-01-17 | Kite Pharma, Inc. | Improving immune cell function |
| EP4323943A1 (en) | 2021-04-16 | 2024-02-21 | Kite Pharma, Inc. | Methods and systems for scheduling a patient-specific immunotherapy procedure |
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| US12357646B2 (en) | 2021-05-14 | 2025-07-15 | Kite Pharma, Inc. | Chimeric antigen receptor T cell therapy |
| CA3221231A1 (en) | 2021-05-24 | 2022-12-01 | Kite Pharma, Inc. | Chimeric antigen receptor |
| WO2022261061A1 (en) | 2021-06-08 | 2022-12-15 | Kite Pharma, Inc. | Gpc3 binding molecules |
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| WO2023278553A1 (en) | 2021-07-01 | 2023-01-05 | Kite Pharma, Inc. | Closed-system and method for autologous and allogeneic cell therapy manufacturing |
| KR20240028503A (ko) | 2021-07-02 | 2024-03-05 | 카이트 파마 인코포레이티드 | 세포 요법 응용 분야에 사용되는 유전자 작제물의 유전자 산물에서 변이체를 식별하는 방법 |
| WO2023009989A1 (en) | 2021-07-26 | 2023-02-02 | Kite Pharma, Inc. | Split chimeric antigen receptors and methods of use |
| JP7791305B2 (ja) | 2021-07-30 | 2025-12-23 | カイト ファーマ インコーポレイテッド | 細胞療法により誘発された毒性の監視及び管理 |
| WO2023069936A1 (en) | 2021-10-18 | 2023-04-27 | Kite Pharma, Inc. | Signaling domains for chimeric antigen receptors |
| CN118742810A (zh) | 2022-02-15 | 2024-10-01 | 凯德药业股份有限公司 | 从免疫疗法预测不良事件 |
| WO2023247324A1 (en) | 2022-06-24 | 2023-12-28 | Cytovac A/S | Novel combination treatment with adoptive cellular therapy |
| US20240091261A1 (en) | 2022-08-26 | 2024-03-21 | Kite Pharma, Inc. | Immune cell function |
| KR20250097844A (ko) | 2022-10-28 | 2025-06-30 | 카이트 파마 인코포레이티드 | 면역요법의 효능 향상 및 지속적인 반응 |
| TW202530421A (zh) | 2022-10-28 | 2025-08-01 | 美商凱特製藥公司 | 用於將免疫療法最佳化之因素 |
| KR20250162836A (ko) | 2023-03-17 | 2025-11-19 | 카이트 파마 인코포레이티드 | 종양 미세환경이 면역요법의 효능에 미치는 영향 |
| US20250161361A1 (en) | 2023-11-01 | 2025-05-22 | Kite Pharma, Inc. | Factors for optimizing immunotherapy efficacy |
| US20250262300A1 (en) | 2024-02-16 | 2025-08-21 | Kite Pharma, Inc. | Systems, methods, and kits for generating and administering engineered t cells and cd38 targeting compounds as a combination therapy |
| US20250290154A1 (en) | 2024-03-04 | 2025-09-18 | Kite Pharma, Inc. | Predictive markers for immunotherapy |
| WO2025231376A1 (en) | 2024-05-03 | 2025-11-06 | Kite Pharma, Inc. | Chimeric receptors binding to cll-1 and methods of use thereof |
| WO2025250819A1 (en) | 2024-05-31 | 2025-12-04 | Kite Pharma, Inc. | Tricistronic constructs for anti-gpc3 car |
| US20260009001A1 (en) | 2024-07-08 | 2026-01-08 | Kite Pharma, Inc. | Systems and methods for applying mechanical force to living cells to generate a phenotypic response |
| WO2026084968A1 (en) | 2024-10-14 | 2026-04-23 | Kite Pharma, Inc. | Biomarker based standards and methods of use |
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| WO2003012085A1 (en) * | 2001-07-30 | 2003-02-13 | Sigma-Tau Industrie Farmaceutiche Riunite S.P.A. | Antigen presenting cells, method for their preparation and their use for cancer vaccines |
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| US20180036397A1 (en) | 2018-02-08 |
| EP2102331A1 (en) | 2009-09-23 |
| PL2102331T3 (pl) | 2012-01-31 |
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