PT2102331E - Vacina antitumoral derivada de células cd4+ normais modificadas quimicamente - Google Patents
Vacina antitumoral derivada de células cd4+ normais modificadas quimicamente Download PDFInfo
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- PT2102331E PT2102331E PT08701232T PT08701232T PT2102331E PT 2102331 E PT2102331 E PT 2102331E PT 08701232 T PT08701232 T PT 08701232T PT 08701232 T PT08701232 T PT 08701232T PT 2102331 E PT2102331 E PT 2102331E
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| US8666674B2 (en) | 2009-07-28 | 2014-03-04 | Promising Future, Llc | Pairing processes for preparing reactive cytotoxic T cells |
| US8467973B2 (en) * | 2009-07-28 | 2013-06-18 | Promising Future, Llc | Pairing processes for preparing alloreactive cytotoxic T cells |
| CN102597222B (zh) * | 2009-10-27 | 2015-07-15 | 因缪尼卡姆股份公司 | 用于增殖抗原特异性t细胞的方法 |
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| US10426740B1 (en) | 2010-08-18 | 2019-10-01 | Avm Biotechnology, Llc | Compositions and methods to inhibit stem cell and progenitor cell binding to lymphoid tissue and for regenerating germinal centers in lymphatic tissues |
| IL255887B (en) | 2015-05-28 | 2022-09-01 | Kite Pharma Inc | Methods for training patients for t-cell therapy |
| CN107921064A (zh) | 2015-05-28 | 2018-04-17 | 凯德药业股份有限公司 | 用于t细胞疗法的诊断方法 |
| SG11201802966TA (en) | 2015-10-20 | 2018-05-30 | Kite Pharma Inc | Methods of preparing t cells for t cell therapy |
| US10603380B2 (en) | 2016-04-01 | 2020-03-31 | Kite Pharma, Inc. | Chimeric antigen and T cell receptors and methods of use |
| KR102608405B1 (ko) | 2016-04-01 | 2023-12-01 | 카이트 파마 인코포레이티드 | 키메라 수용체 및 그의 사용 방법 |
| US10689450B2 (en) | 2016-04-01 | 2020-06-23 | Kite Pharma, Inc | BCMA binding molecules and methods of use thereof |
| US11046775B2 (en) | 2017-02-14 | 2021-06-29 | Kite Pharma, Inc. | CD70 binding molecules and methods of use thereof |
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| ES2954311T3 (es) | 2017-04-01 | 2023-11-21 | Avm Biotechnology Llc | Reemplazo de un preacondicionamiento citotóxico antes de una inmunoterapia celular |
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| PT3615146T (pt) | 2017-04-24 | 2023-11-27 | Kite Pharma Inc | Domínios de ligação ao antigénio humanizados contra cd19 e métodos de utilização |
| TWI862477B (zh) | 2017-05-26 | 2024-11-21 | 美商凱特製藥公司 | 製備和使用胚胎間充質先驅細胞的方法 |
| KR102521245B1 (ko) | 2017-09-15 | 2023-04-17 | 카이트 파마 인코포레이티드 | 관리 연속성 및 신원 연속성 생물학적 샘플 추적으로 환자-특이적 면역요법 절차를 수행하기 위한 방법 및 시스템 |
| ES2980055T3 (es) | 2017-09-22 | 2024-09-27 | Kite Pharma Inc | Polipéptidos quiméricos y usos de los mismos |
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| WO2019099707A1 (en) | 2017-11-16 | 2019-05-23 | Kite Pharma, Inc | Modified chimeric antigen receptors and methods of use |
| KR20200110356A (ko) | 2018-01-15 | 2020-09-23 | 화이자 인코포레이티드 | 키메라 항원 수용체 면역요법을 4-1bb 효능제와 조합하여 투여하는 방법 |
| WO2019152957A1 (en) | 2018-02-02 | 2019-08-08 | Arizona Board Of Regents On Behalf Of Arizona State University | Dna-chimeric antigen receptor t cells for immunotherapy |
| JP2021513839A (ja) | 2018-02-16 | 2021-06-03 | カイト ファーマ インコーポレイテッドＫｉｔｅ Ｐｈａｒｍａ， Ｉｎｃ | 改変された多能性幹細胞並びに製造方法及び使用方法 |
| CA3291067A1 (en) | 2018-04-12 | 2025-11-29 | Kite Pharma, Inc. | Chimeric receptor t cell treatment using characteristics of the tumor microenvironment |
| SG11202011541SA (en) | 2018-06-01 | 2020-12-30 | Kite Pharma Inc | Chimeric antigen receptor t cell therapy |
| WO2019243888A1 (en) | 2018-06-22 | 2019-12-26 | Kite Pharma Eu B.V. | Compositions and methods for making engineered t cells |
| CA3107938C (en) | 2018-08-02 | 2024-04-30 | Kite Pharma, Inc. | Chimeric antigen receptor therapy t cell expansion kinetics and uses thereof |
| EP3632446B3 (en) | 2018-10-03 | 2024-01-24 | AVM Biotechnology, LLC | Immunoablative therapies |
| EP3488851A1 (en) | 2018-10-03 | 2019-05-29 | AVM Biotechnology, LLC | Immunoablative therapies |
| US11793834B2 (en) | 2018-12-12 | 2023-10-24 | Kite Pharma, Inc. | Chimeric antigen and T cell receptors and methods of use |
| EP3953454A1 (en) | 2019-04-12 | 2022-02-16 | CytoVac A/S | Method for preparation of cancer/testis antigen-specific t-cells |
| AU2020267378B2 (en) | 2019-05-03 | 2025-01-30 | Kite Pharma, Inc. | Methods of administering chimeric antigen receptor immunotherapy |
| US12109235B2 (en) | 2019-06-21 | 2024-10-08 | Kite Pharma, Inc. | TGF-beta receptors and methods of use |
| BR112022003852A2 (pt) | 2019-09-03 | 2022-05-31 | Allogene Therapeutics Inc | Métodos de preparação de células t para terapia de células t |
| IL292665B2 (en) | 2019-11-06 | 2026-02-01 | Kite Pharma Inc | Chimeric antigen receptor T cell therapy |
| WO2021260675A1 (en) | 2020-06-24 | 2021-12-30 | Yeda Research And Development Co. Ltd. | Agents for sensitizing solid tumors to treatment |
| KR20230084470A (ko) | 2020-08-14 | 2023-06-13 | 카이트 파마 인코포레이티드 | 면역 세포 기능의 향상 |
| EP4501951A3 (en) | 2020-08-25 | 2025-04-30 | Kite Pharma, Inc. | T cells with improved functionality |
| EP4237854A1 (en) | 2020-10-28 | 2023-09-06 | Kite Pharma, Inc. | Flow cytometric method for characterization of t-cell impurities |
| KR20230124656A (ko) | 2020-12-24 | 2023-08-25 | 카이트 파마 인코포레이티드 | 전립선암 키메라 항원 수용체 |
| AU2022205653B2 (en) | 2021-01-10 | 2025-05-22 | Kite Pharma, Inc. | T cell therapy |
| EP4281545A1 (en) | 2021-01-21 | 2023-11-29 | CytoVac A/S | Method for t-cell expansion and related medical applications |
| EP4294950A1 (en) | 2021-02-20 | 2023-12-27 | Kite Pharma, Inc. | Gene markers for selecting immunotherapies |
| WO2022192439A1 (en) | 2021-03-11 | 2022-09-15 | Kite Pharma, Inc. | Improving immune cell function |
| CA3216056A1 (en) | 2021-04-16 | 2022-10-20 | Kite Pharma, Inc. | Taci/bcma dual binding molecules |
| WO2022221126A1 (en) | 2021-04-16 | 2022-10-20 | Kite Pharma, Inc. | Methods and systems for scheduling a patient-specific immunotherapy procedure |
| IL308227A (en) | 2021-05-14 | 2024-01-01 | Kite Pharma Inc | Chimeric antigen receptor t cell therapy |
| US12331315B2 (en) | 2021-05-24 | 2025-06-17 | Kite Pharma, Inc. | Chimeric antigen receptor |
| CN117730100A (zh) | 2021-06-08 | 2024-03-19 | 凯德药业股份有限公司 | Gpc3结合分子 |
| WO2022269019A1 (en) | 2021-06-25 | 2022-12-29 | Cytovac A/S | Method for preparation of cytotoxic t lymphocytes with broad tumour-specific reactivity and characteristics of early differentiation cells |
| EP4363558A1 (en) | 2021-07-01 | 2024-05-08 | Kite Pharma, Inc. | Closed-system and method for autologous and allogeneic cell therapy manufacturing |
| EP4364151A1 (en) | 2021-07-02 | 2024-05-08 | Kite Pharma, Inc. | A method for identifying variants in gene products from gene constructs used in cell therapy applications |
| WO2023009989A1 (en) | 2021-07-26 | 2023-02-02 | Kite Pharma, Inc. | Split chimeric antigen receptors and methods of use |
| US20230268031A1 (en) | 2021-07-30 | 2023-08-24 | Kite Pharma, Inc. | Monitoring and management of cell therapy-induced toxicities |
| AR127745A1 (es) | 2021-10-18 | 2024-02-28 | Kite Pharma Inc | Dominios de señalización para receptores quiméricos de antígenos |
| US20230296610A1 (en) | 2022-02-15 | 2023-09-21 | Kite Pharma, Inc. | Predicting adverse events from immunotherapy |
| WO2023247324A1 (en) | 2022-06-24 | 2023-12-28 | Cytovac A/S | Novel combination treatment with adoptive cellular therapy |
| WO2024044670A1 (en) | 2022-08-26 | 2024-02-29 | Kite Pharma, Inc. | Improving immune cell function |
| TW202530421A (zh) | 2022-10-28 | 2025-08-01 | 美商凱特製藥公司 | 用於將免疫療法最佳化之因素 |
| KR20250097844A (ko) | 2022-10-28 | 2025-06-30 | 카이트 파마 인코포레이티드 | 면역요법의 효능 향상 및 지속적인 반응 |
| IL323071A (en) | 2023-03-17 | 2025-10-01 | Kite Pharma Inc | The influence of the tumor microenvironment on the effectiveness of immunotherapy |
| US20250161361A1 (en) | 2023-11-01 | 2025-05-22 | Kite Pharma, Inc. | Factors for optimizing immunotherapy efficacy |
| WO2025174963A1 (en) | 2024-02-16 | 2025-08-21 | Kite Pharma, Inc. | Systems, methods, and kits for generating and administering engineered t cells and cd38 targeting compounds as a combination therapy |
| US20250290154A1 (en) | 2024-03-04 | 2025-09-18 | Kite Pharma, Inc. | Predictive markers for immunotherapy |
| TW202604957A (zh) | 2024-05-03 | 2026-02-01 | 美商凱特製藥公司 | 嵌合受體及其使用方法 |
| WO2025250819A1 (en) | 2024-05-31 | 2025-12-04 | Kite Pharma, Inc. | Tricistronic constructs for anti-gpc3 car |
| TW202607133A (zh) | 2024-07-08 | 2026-02-16 | 美商凱特製藥公司 | 用於對活細胞施加機械力以產生表型反應的系統及方法 |
| WO2026084968A1 (en) | 2024-10-14 | 2026-04-23 | Kite Pharma, Inc. | Biomarker based standards and methods of use |
| WO2026096859A2 (en) | 2024-11-04 | 2026-05-07 | Kite Pharma, Inc. | Biomarkers related to immunotherapy efficacy |
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| PL2102331T3 (pl) | 2012-01-31 |
| AU2008203730B2 (en) | 2013-11-07 |
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