PL2327718T3 - Ulepszone systemy ekspresyjne z wykorzystaniem systemu sekrecji SEC - Google Patents

Ulepszone systemy ekspresyjne z wykorzystaniem systemu sekrecji SEC

Info

Publication number
PL2327718T3
PL2327718T3 PL10179615.9T PL10179615T PL2327718T3 PL 2327718 T3 PL2327718 T3 PL 2327718T3 PL 10179615 T PL10179615 T PL 10179615T PL 2327718 T3 PL2327718 T3 PL 2327718T3
Authority
PL
Poland
Prior art keywords
sec
expression systems
improved expression
system secretion
secretion
Prior art date
Application number
PL10179615.9T
Other languages
English (en)
Inventor
Diane M. Retallack
Jane C. Schneider
Thomas Martin Ramseier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of PL2327718T3 publication Critical patent/PL2327718T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
PL10179615.9T 2003-11-21 2004-11-22 Ulepszone systemy ekspresyjne z wykorzystaniem systemu sekrecji SEC PL2327718T3 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US52412403P 2003-11-21 2003-11-21

Publications (1)

Publication Number Publication Date
PL2327718T3 true PL2327718T3 (pl) 2016-10-31

Family

ID=34994140

Family Applications (2)

Application Number Title Priority Date Filing Date
PL10179651T PL2336153T3 (pl) 2003-11-21 2004-11-22 Udoskonalone systemy ekspresji z układem wydzielania SEC
PL10179615.9T PL2327718T3 (pl) 2003-11-21 2004-11-22 Ulepszone systemy ekspresyjne z wykorzystaniem systemu sekrecji SEC

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PL10179651T PL2336153T3 (pl) 2003-11-21 2004-11-22 Udoskonalone systemy ekspresji z układem wydzielania SEC

Country Status (11)

Country Link
US (1) US7985564B2 (pl)
EP (4) EP1687324B1 (pl)
JP (1) JP5028551B2 (pl)
KR (1) KR101265343B1 (pl)
CN (1) CN1882605B (pl)
AU (1) AU2004317306B2 (pl)
BR (1) BRPI0416807A (pl)
CA (1) CA2546157C (pl)
MX (1) MXPA06005705A (pl)
PL (2) PL2336153T3 (pl)
WO (1) WO2005089093A2 (pl)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
AU2004317306B2 (en) 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
JP2008507294A (ja) 2004-07-26 2008-03-13 ダウ グローバル テクノロジーズ インコーポレイティド 菌株遺伝子操作による改善されたタンパク質発現のための方法
JP5130662B2 (ja) * 2006-06-07 2013-01-30 Ｊｎｃ株式会社 タンパク質の可溶性タンパク質としての製造方法
EP2108047B1 (en) * 2007-01-31 2012-10-24 Pfenex Inc. Bacterial leader sequences for increased expression
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2142651B1 (en) * 2007-04-27 2013-05-22 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US8318481B2 (en) 2007-12-07 2012-11-27 Pfenex Inc. High copy number self-replicating plasmids in pseudomonas
WO2010008764A1 (en) * 2008-06-23 2010-01-21 Dow Global Technologies Inc. Pseudomonas fluorescens strains for production of extracellular recombinant protein
CN102459315B (zh) 2009-04-17 2016-03-02 陶氏益农公司 Dig-3杀虫cry毒素
EP2512222B1 (en) 2009-12-16 2018-01-24 Dow AgroSciences LLC COMBINED USE OF CRY1Ca AND CRY1Fa PROTEINS FOR INSECT RESISTANCE MANAGEMENT
RU2573909C2 (ru) 2010-03-04 2016-01-27 Пфенекс Инк. Способ получения растворимого рекомбинантного белка интерферона без денатурирования
WO2011126811A2 (en) 2010-03-30 2011-10-13 Pfenex Inc. High level expression of recombinant toxin proteins
AU2011235210B2 (en) 2010-04-01 2015-07-16 Pfenex Inc. Methods for G-CSF production in a Pseudomonas host cell
WO2011133895A1 (en) 2010-04-23 2011-10-27 Dow Agrosciences Llc Combinations including cry34ab/35ab and cry6aaproteins to prevent development of resistance corn rootworms(diabrotica spp.)
US9234208B1 (en) 2010-05-10 2016-01-12 Dow Agrosciences Llc DIG-13 insecticidal cry toxins
AR084293A1 (es) 2010-12-16 2013-05-08 Dow Agrosciences Llc Cry1FA RADIOMARCADA, BIOLOGICAMENTE ACTIVA Y METODOS DE ENSAYOS DE UNION AL RECEPTOR
SG11201404991YA (en) 2012-02-23 2014-09-26 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
EP2841590A4 (en) * 2012-04-27 2016-03-23 Pronutria Inc NUCLEIC ACIDS, CELLS AND METHODS FOR PRODUCING SECRETED PROTEINS
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
CN103254277B (zh) * 2013-05-03 2017-02-08 南京工业大学 强分泌性信号肽增强小肽模序及其应用
TW201542093A (zh) 2014-03-21 2015-11-16 艾格里遺傳學股份有限公司 用於控制玉米穗蟲之Ｃｒｙ１Ｄ
CA2950947A1 (en) 2014-06-20 2015-12-23 Dow Agrosciences Llc Vegetative insecticidal proteins useful for control of insect pests
MX2017006866A (es) 2014-12-01 2017-11-15 Pfenex Inc Pares de unión para producción de péptidos.
AU2015372474B2 (en) 2014-12-30 2019-02-14 Dow Agrosciences Llc Modified Cry1Ca toxins useful for control of insect pests
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
CA3038972A1 (en) 2016-09-30 2018-04-05 Dow Agrosciences Llc Binary insecticidal cry toxins
WO2018197949A1 (en) 2017-04-27 2018-11-01 Juno Therapeutics Gmbh Oligomeric particle reagents and methods of use thereof
AU2018354069A1 (en) * 2017-10-27 2020-06-11 Pelican Technology Holdings, Inc. Bacterial leader sequences for periplasmic protein expression
EP3700921A4 (en) 2017-10-27 2021-12-15 Pfenex Inc. PROCESS FOR THE PRODUCTION OF RECOMBINANT ERWINIA ASPARAGINASE
CN113481226B (zh) * 2018-08-07 2022-06-21 康码（上海）生物科技有限公司 信号肽相关序列及其在蛋白质合成中的应用
CN112812176B (zh) * 2021-01-13 2022-10-11 江南大学 一种通过低盐絮凝法从螺旋藻中提取藻蓝蛋白的方法
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4595658A (en) 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4513600A (en) 1983-01-03 1985-04-30 The Minster Machine Company Cam actuated ejector for a shell press
GB8308483D0 (en) 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4637980A (en) 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
US5288852A (en) * 1984-03-06 1994-02-22 Dainippon Pharmaceutical Co., Ltd. Human tumor necrosis factor polypeptides
EP0155189A3 (en) 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
ATE78515T1 (de) 1984-10-05 1992-08-15 Genentech Inc Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.
US4963495A (en) 1984-10-05 1990-10-16 Genentech, Inc. Secretion of heterologous proteins
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
GB8529014D0 (en) 1985-11-25 1986-01-02 Biogen Nv Enhanced secretion of heterologous proteins
US5232840A (en) 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
ATE109828T1 (de) 1987-04-23 1994-08-15 Monsanto Co Sekretion eines dem insulin ähnlichen wachstumsfaktors in e. coli.
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
AU3751689A (en) 1988-05-09 1989-11-29 United States of America, as represented by the Secretary, U.S. Department of Commerce, The Vector for secretion of proteins directly into periplasm or culture medium
US5169772A (en) 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
US6780613B1 (en) 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
US5175099A (en) 1989-05-17 1992-12-29 Research Corporation Technologies, Inc. Retrovirus-mediated secretion of recombinant products
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
FI913434A (fi) 1990-07-16 1992-01-17 Technical Research Centre F Finland Rekombinanta fusionsproteiner vilka utsoendras.
US5417971A (en) 1991-10-22 1995-05-23 University Of Saskatchewan Vaccines for Actinobacillus pleuropneumoniae
US5348867A (en) 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
US5563046A (en) 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
US5914254A (en) 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US6171823B1 (en) 1994-12-09 2001-01-09 Novo Nordisk A/S Process for producing extracellular proteins in bacteria
US6495357B1 (en) 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
IT1283225B1 (it) * 1996-03-11 1998-04-16 Renata Maria Anna Ve Cavaliere Ceppi di batteri, composizione farmaceutica contenente uno o piu' di tali ceppi e uso dei medesimi per la prevenzione e la terapia delle
DE69738723D1 (de) * 1996-08-16 2008-07-03 Genencor Int Expressionssystem, abgeleitet von der Lipase-Regulationskaskade von Pseudomonas alcaligenes.
US6361989B1 (en) 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
US6551795B1 (en) * 1998-02-18 2003-04-22 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to pseudomonas aeruginosa for diagnostics and therapeutics
US6156552A (en) 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
US20030064435A1 (en) 1998-05-28 2003-04-03 Weiner Joel Hirsch Compositions and methods for protein secretion
US6323007B1 (en) 1998-09-18 2001-11-27 Novozymes A/S 2,6-β-D-fructan hydrolase enzyme and processes for using the enzyme
ES2318070T3 (es) 1998-10-28 2009-05-01 Genentech, Inc. Procedimiento para la recuperacion facilitada de proteinas heterologas a partir de celulas bacterianas.
KR100312456B1 (ko) * 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
MXPA01009699A (es) 1999-03-29 2002-05-14 Novo Nordisk As Polipeptidos que tienen actividad de enzima ramificadora y acidos nucleicos que los codifican.
US6664386B1 (en) 1999-04-08 2003-12-16 The Research Foundation Of State University Of New York System for efficient secretion of recombinant proteins
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
DE19944870A1 (de) 1999-09-18 2001-03-29 Aventis Pharma Gmbh Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
US6617143B1 (en) 1999-10-20 2003-09-09 Novozymes A/S Polypeptides having glucanotransferase activity and nucleic acids encoding same
US6509181B1 (en) 2000-04-14 2003-01-21 Novozymes, A/S Polypeptides having haloperoxide activity
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
GB0027782D0 (en) 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods fro large scale protein productio in prokaryotes
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
DE10108212A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
AU2002306484A1 (en) 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
WO2003079007A1 (en) 2002-03-15 2003-09-25 The Trustees Of The University Of Pennsylvania Heterologous protein production using the twin arginine translocation pathway
KR20050016362A (ko) 2002-04-22 2005-02-21 다우 글로벌 테크놀로지스 인크. 펩티드의 저비용 생산
DE60325611D1 (de) 2002-07-03 2009-02-12 Dow Global Technologies Inc Benzoat- und antranilatinduzierbare promotoren
AU2004317306B2 (en) 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
KR101183720B1 (ko) * 2004-01-16 2012-09-17 다우 글로벌 테크놀로지스 엘엘씨 슈도모나스 플루오레센스에서의 포유류 단백질의 발현
EP2108047B1 (en) 2007-01-31 2012-10-24 Pfenex Inc. Bacterial leader sequences for increased expression

Also Published As

Publication number Publication date
JP2007512028A (ja) 2007-05-17
BRPI0416807A (pt) 2007-01-09
AU2004317306B2 (en) 2010-09-02
EP1687324A2 (en) 2006-08-09
EP2327718B1 (en) 2016-03-23
EP2336153B1 (en) 2016-03-30
MXPA06005705A (es) 2006-08-23
WO2005089093A2 (en) 2005-09-29
CA2546157A1 (en) 2005-09-29
KR101265343B1 (ko) 2013-05-20
PL2336153T3 (pl) 2016-08-31
CN1882605B (zh) 2012-07-11
JP5028551B2 (ja) 2012-09-19
CA2546157C (en) 2014-07-22
EP2327718A1 (en) 2011-06-01
US7985564B2 (en) 2011-07-26
WO2005089093A3 (en) 2006-03-02
EP2314602A1 (en) 2011-04-27
EP1687324A4 (en) 2007-08-22
US20060008877A1 (en) 2006-01-12
EP2336153A1 (en) 2011-06-22
EP1687324B1 (en) 2012-08-22
KR20060123173A (ko) 2006-12-01
CN1882605A (zh) 2006-12-20
AU2004317306A1 (en) 2005-09-29

Similar Documents

Publication Publication Date Title
EP1687324A4 (en) IMPROVED EXPRESSION SYSTEM WITH SEC SYSTEM SECRETION
ZA200605862B (en) PGRO Expression Units
GB2409502B (en) Connection
GB0305873D0 (en) Get into bed - chair
GB0325494D0 (en) Expression system
SI1533429T1 (sl) Spoj
GB0304882D0 (en) Polypetide
GB0323933D0 (en) The zap-o
GB0302146D0 (en) Hi-Safe systems
GB0326461D0 (en) Stop off
GB0315881D0 (en) The sunwatch
GB0326573D0 (en) The harmeraid
GB0308790D0 (en) The degravitator
GB0309289D0 (en) The degravitator
GB0310830D0 (en) The Teewister
GB0313665D0 (en) The degravitator
GB0313688D0 (en) The dry-warm unbrella
GB0306818D0 (en) The slumber-bumper
GB0300669D0 (en) The babybath
GB0314407D0 (en) The memopage
GB0308271D0 (en) The Railbin
GB0306819D0 (en) The seaside-snoozer
GB0319442D0 (en) The bedrite sleeptite
GB0322562D0 (en) The matlight
GB0307940D0 (en) The degravitator