NZ537939A - Methods using O6-alkylguanine-DNA alkyltransferases for labelling a protein of interest which is fused to the AGT - Google Patents
Methods using O6-alkylguanine-DNA alkyltransferases for labelling a protein of interest which is fused to the AGTInfo
- Publication number
- NZ537939A NZ537939A NZ537939A NZ53793902A NZ537939A NZ 537939 A NZ537939 A NZ 537939A NZ 537939 A NZ537939 A NZ 537939A NZ 53793902 A NZ53793902 A NZ 53793902A NZ 537939 A NZ537939 A NZ 537939A
- Authority
- NZ
- New Zealand
- Prior art keywords
- fusion protein
- agt
- label
- protein
- alkylguanine
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 74
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 66
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 62
- 108010014722 Alkyl and Aryl Transferases Proteins 0.000 title claims abstract description 29
- 102000002226 Alkyl and Aryl Transferases Human genes 0.000 title claims abstract description 29
- 238000002372 labelling Methods 0.000 title claims description 28
- 108020001507 fusion proteins Proteins 0.000 claims abstract description 127
- 102000037865 fusion proteins Human genes 0.000 claims abstract description 127
- 239000000758 substrate Substances 0.000 claims abstract description 54
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- 238000001727 in vivo Methods 0.000 claims description 17
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 16
- 230000014509 gene expression Effects 0.000 claims description 14
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- 108010090804 Streptavidin Proteins 0.000 claims description 8
- 238000007792 addition Methods 0.000 claims description 8
- 125000000217 alkyl group Chemical group 0.000 claims description 8
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- 239000011616 biotin Substances 0.000 claims description 8
- XWNJMSJGJFSGRY-UHFFFAOYSA-N 2-(benzylamino)-3,7-dihydropurin-6-one Chemical compound N1C=2N=CNC=2C(=O)N=C1NCC1=CC=CC=C1 XWNJMSJGJFSGRY-UHFFFAOYSA-N 0.000 claims description 7
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- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims description 4
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims description 4
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- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 3
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- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 2
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- KRWMERLEINMZFT-UHFFFAOYSA-N O6-benzylguanine Chemical class C=12NC=NC2=NC(N)=NC=1OCC1=CC=CC=C1 KRWMERLEINMZFT-UHFFFAOYSA-N 0.000 description 1
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- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
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- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
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- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 1
- CPTBDICYNRMXFX-UHFFFAOYSA-N procarbazine Chemical compound CNNCC1=CC=C(C(=O)NC(C)C)C=C1 CPTBDICYNRMXFX-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NZ540410A NZ540410A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28276601P | 2001-04-10 | 2001-04-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ537939A true NZ537939A (en) | 2005-08-26 |
Family
ID=23083030
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ537939A NZ537939A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases for labelling a protein of interest which is fused to the AGT |
| NZ540410A NZ540410A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
| NZ528594A NZ528594A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ540410A NZ540410A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
| NZ528594A NZ528594A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7939284B2 (enExample) |
| EP (4) | EP1410023B1 (enExample) |
| JP (2) | JP4195815B2 (enExample) |
| KR (1) | KR20040039194A (enExample) |
| CN (2) | CN1295510C (enExample) |
| AT (3) | ATE419378T1 (enExample) |
| AU (1) | AU2002251257B2 (enExample) |
| CA (1) | CA2443570A1 (enExample) |
| DE (3) | DE60230689D1 (enExample) |
| DK (1) | DK1410023T3 (enExample) |
| IL (1) | IL157880A0 (enExample) |
| NZ (3) | NZ537939A (enExample) |
| WO (1) | WO2002083937A2 (enExample) |
| ZA (1) | ZA200307442B (enExample) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004031404A1 (en) | 2002-10-03 | 2004-04-15 | Ecole Polytechnique Federale De Lausanne (Epfl) | Protein labelling with o6-alkylguanine-dna alkyltransferase |
| US7799524B2 (en) | 2002-10-03 | 2010-09-21 | Ecole Polytechnique Ferdeale de Lausanne | Substrates for O6-alkylguanina-DNA alkyltransferase |
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