ATE419378T1 - Verfahren zur verwendung von o6-alkylguanin-dns alkyltransferasen - Google Patents

Verfahren zur verwendung von o6-alkylguanin-dns alkyltransferasen

Info

Publication number
ATE419378T1
ATE419378T1 AT06111733T AT06111733T ATE419378T1 AT E419378 T1 ATE419378 T1 AT E419378T1 AT 06111733 T AT06111733 T AT 06111733T AT 06111733 T AT06111733 T AT 06111733T AT E419378 T1 ATE419378 T1 AT E419378T1
Authority
AT
Austria
Prior art keywords
cell
alkylguanine
agt
protein
label
Prior art date
Application number
AT06111733T
Other languages
German (de)
English (en)
Inventor
Kai Johnsson
Susanne Gendreizig
Antje Keppler
Original Assignee
Ecole Polytech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecole Polytech filed Critical Ecole Polytech
Application granted granted Critical
Publication of ATE419378T1 publication Critical patent/ATE419378T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT06111733T 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dns alkyltransferasen ATE419378T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28276601P 2001-04-10 2001-04-10

Publications (1)

Publication Number Publication Date
ATE419378T1 true ATE419378T1 (de) 2009-01-15

Family

ID=23083030

Family Applications (3)

Application Number Title Priority Date Filing Date
AT06111733T ATE419378T1 (de) 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dns alkyltransferasen
AT08168182T ATE482395T1 (de) 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dns alkyltransferasen
AT02720187T ATE331220T1 (de) 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dna alkyltransferasen

Family Applications After (2)

Application Number Title Priority Date Filing Date
AT08168182T ATE482395T1 (de) 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dns alkyltransferasen
AT02720187T ATE331220T1 (de) 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dna alkyltransferasen

Country Status (14)

Country Link
US (2) US7939284B2 (enExample)
EP (4) EP1410023B1 (enExample)
JP (2) JP4195815B2 (enExample)
KR (1) KR20040039194A (enExample)
CN (2) CN1295510C (enExample)
AT (3) ATE419378T1 (enExample)
AU (1) AU2002251257B2 (enExample)
CA (1) CA2443570A1 (enExample)
DE (3) DE60230689D1 (enExample)
DK (1) DK1410023T3 (enExample)
IL (1) IL157880A0 (enExample)
NZ (3) NZ537939A (enExample)
WO (1) WO2002083937A2 (enExample)
ZA (1) ZA200307442B (enExample)

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US7799524B2 (en) 2002-10-03 2010-09-21 Ecole Polytechnique Ferdeale de Lausanne Substrates for O6-alkylguanina-DNA alkyltransferase
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JP4748685B2 (ja) 2003-01-31 2011-08-17 プロメガ コーポレイション タンパク質に対する官能基の共有結合的テザリング
AU2004242249A1 (en) * 2003-05-23 2004-12-02 Epfl-Ecole Polytechnique Federale De Lausanne Methods for protein labeling based on acyl carrier protein
EP1730298B1 (en) * 2004-03-02 2015-11-11 EPFL Ecole Polytechnique Fédérale de Lausanne Specific substrates for o6-alkylguanine-dna alkyltransferase
US7888090B2 (en) 2004-03-02 2011-02-15 Ecole Polytechnique Federale De Lausanne Mutants of O6-alkylguanine-DNA alkyltransferase
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7825096B2 (en) 2004-09-08 2010-11-02 The United States Of America As Represented By The Department Of Health And Human Services O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs
US7273714B2 (en) * 2005-03-11 2007-09-25 Vion Pharmaceuticals, Inc. Alkylguanyltransferase assays
US8178314B2 (en) 2005-04-27 2012-05-15 Covalys Biosciences Ag Pyrimidines reacting with O6-alkylguanine-DNA alkyltransferase fusion protein and method for detecting protein
FR2890174B1 (fr) 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
FR2890446B1 (fr) 2005-09-05 2008-04-18 Cis Bio Internat Sa Methode de detection d'interaction intracellulaire entre bio-molecules
CA2656192C (en) 2006-06-30 2016-05-03 Ambit Biosciences Corporation Detectable nucleic acid tag
WO2008054821A2 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
NZ577303A (en) * 2006-12-05 2011-12-22 Lasergen Inc Nucleotides and nucleosides having derivitized bases including a dye and terminating group and uses in DNA sequencing
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
EP1978093A1 (en) * 2007-04-04 2008-10-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method of crosslinking two objects of interest
GB0711560D0 (en) * 2007-06-14 2007-07-25 Innova Biosciences Ltd Improvements in or relating to production of conjugates
EP2210098B1 (en) 2007-10-29 2011-08-31 New England Biolabs, Inc. Methods and compositions for detection and enrichment of target small rnas
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
WO2011091146A1 (en) 2010-01-20 2011-07-28 New England Biolabs, Inc. Compositions, methods and related uses for cleaving modified dna
CA2810725A1 (en) 2010-09-15 2012-03-22 Constance Neely Wilson Methods of use and kit for measurement of lipopolysaccharide with a time resolved fluorescence based assay
EP3670523B1 (en) 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh unblocked, fast photocleavable terminating nucleotides and their use in methods for nucleic acid sequencing
CN104114697A (zh) * 2011-12-09 2014-10-22 巴斯德研究所 多重免疫筛选分析
US9200260B2 (en) 2012-03-15 2015-12-01 New England Biolabs, Inc. Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
JP5781589B2 (ja) * 2013-12-20 2015-09-24 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法
WO2016003795A1 (en) 2014-07-02 2016-01-07 New England Biolabs, Inc. Universal n-glycan binding reagent
JP2017537657A (ja) 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
CN104844710B (zh) * 2015-04-13 2018-02-02 西北大学 定向固定化pega复合型树酯的制备方法及应用
WO2017214151A1 (en) 2016-06-06 2017-12-14 Northwestern University Fusion protein construct
CN113444047B (zh) 2016-07-20 2023-06-16 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN111253417B (zh) * 2019-09-04 2022-03-25 大连民族大学 一种嘌呤衍生物的合成及应用
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ， インク． 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）
EP4103701A1 (en) 2020-02-13 2022-12-21 New England Biolabs, Inc. Variant family d dna polymerases
WO2021163559A1 (en) 2020-02-13 2021-08-19 New England Biolabs, Inc. Variant family d dna polymerases
WO2021183921A1 (en) 2020-03-12 2021-09-16 New England Biolabs, Inc. A rapid diagnostic test for lamp
CN112010983A (zh) * 2020-08-11 2020-12-01 西北大学 止咳平喘中药活性成分靶向筛选方法及试剂盒
WO2022040443A2 (en) 2020-08-21 2022-02-24 New England Biolabs, Inc. A rapid diagnostic test for lamp
EP4347804A1 (en) 2021-05-27 2024-04-10 New England Biolabs, Inc. Dnase i variants, compositions, methods, and kits
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
WO2023097226A2 (en) 2021-11-24 2023-06-01 New England Biolabs, Inc. Double-stranded dna deaminases
WO2023197015A1 (en) 2022-04-08 2023-10-12 New England Biolabs, Inc. Compositions and analysis of dephosphorylated oligoribonucleotides
EP4658673A1 (en) 2023-02-01 2025-12-10 New England Biolabs, Inc. Duplex-specific dnases
WO2025076551A1 (en) 2023-10-05 2025-04-10 New England Biolabs, Inc. Methyl cytosine-selective deaminases and uses thereof
WO2025129193A1 (en) 2023-12-15 2025-06-19 New England Biolabs, Inc. Glycosyltransferases and related methods
WO2025137453A1 (en) 2023-12-20 2025-06-26 New England Biolabs, Inc. Compositions, methods, kits, and instruments for analyzing rna structure
GB202400129D0 (en) 2024-01-04 2024-02-21 Cambridge Entpr Ltd Nucleic acid barcodes
WO2025224010A1 (en) 2024-04-24 2025-10-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Improved o6-alkylguanine-dna alkyltransferase substrates
WO2025227034A1 (en) 2024-04-26 2025-10-30 New England Biolabs, Inc. Salt active nuclease compositions and methods

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GB9224888D0 (en) * 1992-11-27 1993-01-13 Univ Singapore Monoclonal antibody
DK0702683T3 (da) * 1993-06-08 2004-02-02 Cancer Rec Tech Ltd 06-substituerede guaninderivater, fremgangsmåde til fremstilling deraf og anvendelse deraf i behandling af tumorceller
DE19903895A1 (de) * 1999-02-01 2000-08-03 Kai Johnsson Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form

Also Published As

Publication number Publication date
DE60230689D1 (de) 2009-02-12
CN1295510C (zh) 2007-01-17
ATE331220T1 (de) 2006-07-15
JP4226053B2 (ja) 2009-02-18
DE60212642D1 (de) 2006-08-03
JP2008154587A (ja) 2008-07-10
EP1410023B1 (en) 2006-06-21
KR20040039194A (ko) 2004-05-10
JP4195815B2 (ja) 2008-12-17
IL157880A0 (en) 2004-03-28
US20040115130A1 (en) 2004-06-17
NZ528594A (en) 2005-07-29
EP2211177A1 (en) 2010-07-28
EP1696234B1 (en) 2008-12-31
DE60237792D1 (de) 2010-11-04
EP2037271B1 (en) 2010-09-22
NZ537939A (en) 2005-08-26
HK1068406A1 (en) 2005-04-29
CN1975422A (zh) 2007-06-06
US8367361B2 (en) 2013-02-05
ZA200307442B (en) 2004-06-30
DK1410023T3 (da) 2006-10-09
CN1527941A (zh) 2004-09-08
EP2211177B1 (en) 2014-07-16
CN1975422B (zh) 2012-09-05
EP2037271A1 (en) 2009-03-18
EP1696234A1 (en) 2006-08-30
NZ540410A (en) 2006-06-30
DE60212642T2 (de) 2007-06-14
US20110201514A1 (en) 2011-08-18
CA2443570A1 (en) 2002-10-24
AU2002251257B2 (en) 2007-08-30
JP2004532028A (ja) 2004-10-21
EP1410023A2 (en) 2004-04-21
US7939284B2 (en) 2011-05-10
WO2002083937A2 (en) 2002-10-24
ATE482395T1 (de) 2010-10-15
WO2002083937A3 (en) 2004-02-26

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