NO864681L - Fremgangsmaate ved direkte genoverfoering i plastider og mitokondrier. - Google Patents
Fremgangsmaate ved direkte genoverfoering i plastider og mitokondrier.Info
- Publication number
- NO864681L NO864681L NO864681A NO864681A NO864681L NO 864681 L NO864681 L NO 864681L NO 864681 A NO864681 A NO 864681A NO 864681 A NO864681 A NO 864681A NO 864681 L NO864681 L NO 864681L
- Authority
- NO
- Norway
- Prior art keywords
- dna
- protoplasts
- gene
- mitochondria
- plastids
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 96
- 230000009365 direct transmission Effects 0.000 title 1
- 210000001938 protoplast Anatomy 0.000 claims abstract description 104
- 210000002706 plastid Anatomy 0.000 claims abstract description 79
- 210000003470 mitochondria Anatomy 0.000 claims abstract description 59
- 244000052769 pathogen Species 0.000 claims abstract description 12
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 7
- 230000035515 penetration Effects 0.000 claims abstract description 5
- 241000196324 Embryophyta Species 0.000 claims description 140
- 108090000623 proteins and genes Proteins 0.000 claims description 135
- 210000004027 cell Anatomy 0.000 claims description 71
- 108020004414 DNA Proteins 0.000 claims description 57
- 239000004009 herbicide Substances 0.000 claims description 51
- 238000012546 transfer Methods 0.000 claims description 37
- 230000002363 herbicidal effect Effects 0.000 claims description 35
- 239000002609 medium Substances 0.000 claims description 30
- 239000002202 Polyethylene glycol Substances 0.000 claims description 29
- 229920001223 polyethylene glycol Polymers 0.000 claims description 29
- MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical group CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 claims description 27
- 239000000725 suspension Substances 0.000 claims description 23
- 239000001963 growth medium Substances 0.000 claims description 19
- 238000004520 electroporation Methods 0.000 claims description 18
- 229960005091 chloramphenicol Drugs 0.000 claims description 15
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 15
- 238000002635 electroconvulsive therapy Methods 0.000 claims description 15
- 150000005846 sugar alcohols Polymers 0.000 claims description 11
- 230000003115 biocidal effect Effects 0.000 claims description 10
- 244000062793 Sorghum vulgare Species 0.000 claims description 9
- 241000209504 Poaceae Species 0.000 claims description 7
- 235000013339 cereals Nutrition 0.000 claims description 7
- 230000010354 integration Effects 0.000 claims description 6
- 239000003550 marker Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 235000019713 millet Nutrition 0.000 claims description 6
- 244000075850 Avena orientalis Species 0.000 claims description 5
- 241000209056 Secale Species 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 239000011777 magnesium Substances 0.000 claims description 4
- 229910052749 magnesium Inorganic materials 0.000 claims description 4
- 230000008929 regeneration Effects 0.000 claims description 4
- 238000011069 regeneration method Methods 0.000 claims description 4
- 230000010076 replication Effects 0.000 claims description 4
- 235000007319 Avena orientalis Nutrition 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 3
- 240000005979 Hordeum vulgare Species 0.000 claims description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 235000007238 Secale cereale Nutrition 0.000 claims description 3
- 239000011575 calcium Substances 0.000 claims description 3
- 229910052791 calcium Inorganic materials 0.000 claims description 3
- 150000001768 cations Chemical class 0.000 claims description 3
- 230000007910 cell fusion Effects 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 101710163270 Nuclease Proteins 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 230000001413 cellular effect Effects 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 229960000318 kanamycin Drugs 0.000 claims description 2
- 229930027917 kanamycin Natural products 0.000 claims description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 claims description 2
- 229930182823 kanamycin A Natural products 0.000 claims description 2
- 229920001451 polypropylene glycol Polymers 0.000 claims description 2
- 108020004638 Circular DNA Proteins 0.000 claims 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 210000000170 cell membrane Anatomy 0.000 claims 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims 1
- 229920002554 vinyl polymer Polymers 0.000 claims 1
- 230000009466 transformation Effects 0.000 description 52
- 239000013612 plasmid Substances 0.000 description 42
- 239000000243 solution Substances 0.000 description 22
- 108091028043 Nucleic acid sequence Proteins 0.000 description 21
- 239000012634 fragment Substances 0.000 description 21
- 210000003763 chloroplast Anatomy 0.000 description 20
- 108091026890 Coding region Proteins 0.000 description 19
- 101150075980 psbA gene Proteins 0.000 description 18
- 229920001184 polypeptide Polymers 0.000 description 16
- 108090000765 processed proteins & peptides Proteins 0.000 description 16
- 102000004196 processed proteins & peptides Human genes 0.000 description 16
- 102000004190 Enzymes Human genes 0.000 description 14
- 108090000790 Enzymes Proteins 0.000 description 14
- 229940088598 enzyme Drugs 0.000 description 14
- 108020005196 Mitochondrial DNA Proteins 0.000 description 11
- 238000010276 construction Methods 0.000 description 11
- 210000001519 tissue Anatomy 0.000 description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 9
- 229930195725 Mannitol Natural products 0.000 description 9
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 9
- 239000000594 mannitol Substances 0.000 description 9
- 235000010355 mannitol Nutrition 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 8
- 244000061176 Nicotiana tabacum Species 0.000 description 8
- 238000004113 cell culture Methods 0.000 description 8
- 230000002438 mitochondrial effect Effects 0.000 description 8
- 239000013598 vector Substances 0.000 description 8
- 241000208125 Nicotiana Species 0.000 description 7
- 101150055766 cat gene Proteins 0.000 description 7
- 230000004927 fusion Effects 0.000 description 7
- 230000029553 photosynthesis Effects 0.000 description 7
- 238000010672 photosynthesis Methods 0.000 description 7
- 229920000936 Agarose Polymers 0.000 description 6
- 240000008100 Brassica rapa Species 0.000 description 6
- 235000011292 Brassica rapa Nutrition 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 6
- 240000007377 Petunia x hybrida Species 0.000 description 6
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 6
- 239000012526 feed medium Substances 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 108700003860 Bacterial Genes Proteins 0.000 description 5
- 235000011331 Brassica Nutrition 0.000 description 5
- 241000219198 Brassica Species 0.000 description 5
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 5
- 241000208818 Helianthus Species 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000000243 photosynthetic effect Effects 0.000 description 5
- 241000589158 Agrobacterium Species 0.000 description 4
- 244000105624 Arachis hypogaea Species 0.000 description 4
- 240000002791 Brassica napus Species 0.000 description 4
- 235000011293 Brassica napus Nutrition 0.000 description 4
- 108700031407 Chloroplast Genes Proteins 0.000 description 4
- 108010070675 Glutathione transferase Proteins 0.000 description 4
- 239000004471 Glycine Substances 0.000 description 4
- 241000227653 Lycopersicon Species 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 241000219823 Medicago Species 0.000 description 4
- 241000209094 Oryza Species 0.000 description 4
- 102000004020 Oxygenases Human genes 0.000 description 4
- 108090000417 Oxygenases Proteins 0.000 description 4
- 241000207763 Solanum Species 0.000 description 4
- 235000002634 Solanum Nutrition 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 241000219793 Trifolium Species 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000003599 detergent Substances 0.000 description 4
- 235000021186 dishes Nutrition 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 101150074945 rbcL gene Proteins 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 238000004062 sedimentation Methods 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 235000003911 Arachis Nutrition 0.000 description 3
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 241000207199 Citrus Species 0.000 description 3
- 102000005720 Glutathione transferase Human genes 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 3
- 241000209082 Lolium Species 0.000 description 3
- 244000100545 Lolium multiflorum Species 0.000 description 3
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 3
- 241000219833 Phaseolus Species 0.000 description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 229930002875 chlorophyll Natural products 0.000 description 3
- 235000019804 chlorophyll Nutrition 0.000 description 3
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 3
- 235000020971 citrus fruits Nutrition 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 230000003204 osmotic effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 101150010007 psbD gene Proteins 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- JYEUMXHLPRZUAT-UHFFFAOYSA-N 1,2,3-triazine Chemical compound C1=CN=NN=C1 JYEUMXHLPRZUAT-UHFFFAOYSA-N 0.000 description 2
- LUBJCRLGQSPQNN-UHFFFAOYSA-N 1-Phenylurea Chemical compound NC(=O)NC1=CC=CC=C1 LUBJCRLGQSPQNN-UHFFFAOYSA-N 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 2
- 239000005971 1-naphthylacetic acid Substances 0.000 description 2
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 2
- XMTQQYYKAHVGBJ-UHFFFAOYSA-N 3-(3,4-DICHLOROPHENYL)-1,1-DIMETHYLUREA Chemical compound CN(C)C(=O)NC1=CC=C(Cl)C(Cl)=C1 XMTQQYYKAHVGBJ-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000234282 Allium Species 0.000 description 2
- 241000219318 Amaranthus Species 0.000 description 2
- 235000003840 Amygdalus nana Nutrition 0.000 description 2
- 244000296825 Amygdalus nana Species 0.000 description 2
- 235000007119 Ananas comosus Nutrition 0.000 description 2
- 244000099147 Ananas comosus Species 0.000 description 2
- 235000005781 Avena Nutrition 0.000 description 2
- 108010077805 Bacterial Proteins Proteins 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 2
- 240000001548 Camellia japonica Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 241000723377 Coffea Species 0.000 description 2
- 241001507921 Cydonia Species 0.000 description 2
- 235000009807 Cydonia Nutrition 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 241000208175 Daucus Species 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000220223 Fragaria Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 108010093031 Galactosidases Proteins 0.000 description 2
- 102000002464 Galactosidases Human genes 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000219146 Gossypium Species 0.000 description 2
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000209219 Hordeum Species 0.000 description 2
- 241000208278 Hyoscyamus Species 0.000 description 2
- COHYTHOBJLSHDF-UHFFFAOYSA-N Indigo Chemical compound N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 2
- 229920005479 Lucite® Polymers 0.000 description 2
- 235000002262 Lycopersicon Nutrition 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 241000218922 Magnoliophyta Species 0.000 description 2
- 241000220225 Malus Species 0.000 description 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000234295 Musa Species 0.000 description 2
- 241000209117 Panicum Species 0.000 description 2
- 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 description 2
- 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 description 2
- 241000219843 Pisum Species 0.000 description 2
- 235000011432 Prunus Nutrition 0.000 description 2
- 241000220324 Pyrus Species 0.000 description 2
- 241001092459 Rubus Species 0.000 description 2
- 241000209051 Saccharum Species 0.000 description 2
- 235000005775 Setaria Nutrition 0.000 description 2
- 241000232088 Setaria <nematode> Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000208292 Solanaceae Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 235000009392 Vitis Nutrition 0.000 description 2
- 241000219095 Vitis Species 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 210000003855 cell nucleus Anatomy 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 239000003593 chromogenic compound Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 235000018597 common camellia Nutrition 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 2
- 238000005188 flotation Methods 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 101150096947 gshB gene Proteins 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- -1 mannitol) Chemical class 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229930000184 phytotoxin Natural products 0.000 description 2
- 238000003976 plant breeding Methods 0.000 description 2
- 239000003123 plant toxin Substances 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 235000014774 prunus Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- WDLCRTLULMGVMR-QWRGUYRKSA-N (2s)-2-amino-5-[[(2r)-1-(carboxymethylamino)-3-[[4-(ethylamino)-6-(propan-2-ylamino)-1,3,5-triazin-2-yl]sulfanyl]-1-oxopropan-2-yl]amino]-5-oxopentanoic acid Chemical compound CCNC1=NC(NC(C)C)=NC(SC[C@H](NC(=O)CC[C@H](N)C(O)=O)C(=O)NCC(O)=O)=N1 WDLCRTLULMGVMR-QWRGUYRKSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- LGGQQYXFFSOIJY-UHFFFAOYSA-N 2-(3-methylbut-2-enyl)-7h-purin-6-amine Chemical compound CC(C)=CCC1=NC(N)=C2NC=NC2=N1 LGGQQYXFFSOIJY-UHFFFAOYSA-N 0.000 description 1
- 101100301006 Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) cbbL2 gene Proteins 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 240000001592 Amaranthus caudatus Species 0.000 description 1
- 235000009328 Amaranthus caudatus Nutrition 0.000 description 1
- 244000300297 Amaranthus hybridus Species 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 241001106067 Atropa Species 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 241000234670 Bromeliaceae Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000195585 Chlamydomonas Species 0.000 description 1
- 108020004998 Chloroplast DNA Proteins 0.000 description 1
- 241000723343 Cichorium Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 101100015982 Dictyostelium discoideum gcsA gene Proteins 0.000 description 1
- 240000001879 Digitalis lutea Species 0.000 description 1
- 239000005510 Diuron Substances 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 241000195620 Euglena Species 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 241000208152 Geranium Species 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- 108010063907 Glutathione Reductase Proteins 0.000 description 1
- 102100036442 Glutathione reductase, mitochondrial Human genes 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 235000009438 Gossypium Nutrition 0.000 description 1
- 241001232367 Gymnosperma Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 101000635799 Homo sapiens Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 101150062179 II gene Proteins 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 239000005583 Metribuzin Substances 0.000 description 1
- 235000016462 Mimosa pudica Nutrition 0.000 description 1
- 240000001140 Mimosa pudica Species 0.000 description 1
- 241000234615 Musaceae Species 0.000 description 1
- 101100172084 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) egtA gene Proteins 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241001107098 Rubiaceae Species 0.000 description 1
- 102100030852 Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Human genes 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241001106018 Salpiglossis Species 0.000 description 1
- 235000002594 Solanum nigrum Nutrition 0.000 description 1
- 244000061457 Solanum nigrum Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229940100389 Sulfonylurea Drugs 0.000 description 1
- 241000192707 Synechococcus Species 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical class O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 1
- 235000007769 Vetiveria zizanioides Nutrition 0.000 description 1
- 244000284012 Vetiveria zizanioides Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- RQVYBGPQFYCBGX-UHFFFAOYSA-N ametryn Chemical compound CCNC1=NC(NC(C)C)=NC(SC)=N1 RQVYBGPQFYCBGX-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 101150004101 cbbL gene Proteins 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 239000004062 cytokinin Chemical class 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Chemical class C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- QEMZNSKDTGKKFO-UHFFFAOYSA-N dichloromethylurea Chemical compound NC(=O)NC(Cl)Cl QEMZNSKDTGKKFO-UHFFFAOYSA-N 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 108010081495 driselase Proteins 0.000 description 1
- 101150078200 dsbD gene Proteins 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 150000004673 fluoride salts Chemical class 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 101150019860 gshA gene Proteins 0.000 description 1
- 101150094189 gshAB gene Proteins 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical class C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- FOXFZRUHNHCZPX-UHFFFAOYSA-N metribuzin Chemical compound CSC1=NN=C(C(C)(C)C)C(=O)N1N FOXFZRUHNHCZPX-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 108091027963 non-coding RNA Proteins 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000002357 osmotic agent Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 230000037432 silent mutation Effects 0.000 description 1
- ODCWYMIRDDJXKW-UHFFFAOYSA-N simazine Chemical compound CCNC1=NC(Cl)=NC(NCC)=N1 ODCWYMIRDDJXKW-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 229940045136 urea Drugs 0.000 description 1
- 244000045561 useful plants Species 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80101485A | 1985-11-22 | 1985-11-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO864681D0 NO864681D0 (no) | 1986-11-21 |
| NO864681L true NO864681L (no) | 1987-05-25 |
Family
ID=25179963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO864681A NO864681L (no) | 1985-11-22 | 1986-11-21 | Fremgangsmaate ved direkte genoverfoering i plastider og mitokondrier. |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP0223247A3 (da) |
| JP (1) | JPS62155093A (da) |
| CN (1) | CN86107908A (da) |
| AU (1) | AU6555586A (da) |
| BR (1) | BR8605753A (da) |
| DK (1) | DK559586A (da) |
| FI (1) | FI864720A (da) |
| GB (1) | GB2183660B (da) |
| HU (1) | HUT42672A (da) |
| IL (1) | IL80704A0 (da) |
| NO (1) | NO864681L (da) |
| PL (1) | PL262517A1 (da) |
| PT (1) | PT83781B (da) |
| ZA (1) | ZA868828B (da) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0429093B1 (de) * | 1984-05-11 | 2001-08-08 | Syngenta Participations AG | Transformation von Pflanzenerbgut |
| DE3587548T2 (de) * | 1984-12-28 | 1993-12-23 | Bayer Ag | Rekombinante DNA, die in pflanzliche Zellen eingebracht werden kann. |
| ES2039474T3 (es) * | 1986-12-05 | 1993-10-01 | Ciba-Geigy Ag | Procedimineto mejorado para la transformacion de protoplastos vegetales. |
| US5350689A (en) * | 1987-05-20 | 1994-09-27 | Ciba-Geigy Corporation | Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells |
| ES2074999T3 (es) * | 1987-05-20 | 1995-10-01 | Ciba Geigy Ag | Plantas de zea mays y plantas de zea mays transgenicas regeneradas de protoplastos o celulas derivadas de protoplastos. |
| US5693507A (en) * | 1988-09-26 | 1997-12-02 | Auburn University | Genetic engineering of plant chloroplasts |
| US6680426B2 (en) | 1991-01-07 | 2004-01-20 | Auburn University | Genetic engineering of plant chloroplasts |
| GB8901675D0 (en) * | 1989-01-26 | 1989-03-15 | Ici Plc | Inhibitor of gene expression |
| US6376744B1 (en) * | 1996-03-06 | 2002-04-23 | Rutgers, The State University Of New Jersey | Plastid transformation in Arabidopsis thaliana |
| ZA986574B (en) * | 1997-07-23 | 2000-01-12 | Sanford Scient Inc | Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance. |
| US6271444B1 (en) | 1998-07-10 | 2001-08-07 | Calgene Llc | Enhancer elements for increased translation in plant plastids |
| US6492578B1 (en) * | 1998-07-10 | 2002-12-10 | Calgene Llc | Expression of herbicide tolerance genes in plant plastids |
| US6512162B2 (en) | 1998-07-10 | 2003-01-28 | Calgene Llc | Expression of eukaryotic peptides in plant plastids |
| US6541682B1 (en) | 1998-11-12 | 2003-04-01 | Calgene Llc | Plastid transformation of solanaceous plants |
| US6515206B1 (en) | 1998-12-23 | 2003-02-04 | Calgene Llc | Plastid transformation of Brassica |
| WO2001070939A1 (en) * | 2000-03-22 | 2001-09-27 | Icon Genetics, Inc. | Methods for transforming plant plastids and making transplastomic plants |
| DE10014412A1 (de) * | 2000-03-24 | 2001-10-04 | Mpb Cologne Gmbh Molecular Pla | Expressionsvektoren zur Anreicherung eines rekombinant hergestellten Proteins in unterschiedlichen Zellkompartimenten |
| AU2001257300A1 (en) | 2000-04-26 | 2001-11-07 | Monsanto Technology Llc | Method for the transformation of plant cell plastids |
| AU2002306406A1 (en) * | 2001-01-09 | 2002-09-12 | Wyeth | Dna constructs for cytoplasmic and mithochondrial expression and methods of making and using same |
| EP2360179A1 (en) | 2002-03-22 | 2011-08-24 | Bayer BioScience N.V. | Novel bacillus thuringiensis insecticidal proteins |
| WO2007007147A2 (en) | 2005-07-08 | 2007-01-18 | Universidad Nacional Autonoma De Mexico Instituto De Biotecnologia | Novel bacterial proteins with pesticidal activity |
| EP2554674B1 (en) | 2007-12-21 | 2014-11-05 | Keygene N.V. | Trichome specific promoters |
| BRPI1010843A2 (pt) | 2009-06-08 | 2015-09-08 | Nunhems Bv | plantas tolerantes à seca |
| WO2011000498A1 (en) | 2009-07-01 | 2011-01-06 | Bayer Bioscience N.V. | Methods and means for obtaining plants with enhanced glyphosate tolerance |
| MX344585B (es) | 2010-05-28 | 2016-12-20 | Nunhems Bv | Platas con un tamaño de fruto incrementado. |
| AU2011276127B2 (en) | 2010-07-08 | 2015-03-19 | University Of Copenhagen | Glucosinolate transporter protein and uses thereof |
| CN101979559A (zh) * | 2010-10-29 | 2011-02-23 | 复旦大学 | 一种受高温诱导的启动子及其应用 |
| WO2012165961A1 (en) | 2011-05-31 | 2012-12-06 | Keygene N.V. | Pest resistant plants |
| US20140259223A1 (en) | 2011-10-19 | 2014-09-11 | Keygene N.V. | Methods for producing cinnamolide and/or drimendiol |
| CN103013905B (zh) * | 2013-01-19 | 2014-03-26 | 安徽科技学院 | 一种真空酶解法制备苏丹草原生质体的方法 |
| CN103013904A (zh) * | 2013-01-19 | 2013-04-03 | 安徽科技学院 | 一种高粱原生质体酶解制备法 |
| WO2014142647A1 (en) | 2013-03-14 | 2014-09-18 | Wageningen Universiteit | Fungals strains with improved citric acid and itaconic acid production |
| JP6978152B2 (ja) | 2015-09-04 | 2021-12-08 | キージーン ナムローゼ フェンノートシャップ | 複相胞子生殖遺伝子 |
| JP2019129705A (ja) * | 2016-03-31 | 2019-08-08 | 日本たばこ産業株式会社 | 植物に物質を導入する方法 |
| US10982226B2 (en) | 2016-06-20 | 2021-04-20 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Green alga bicarbonate transporter and uses thereof |
| CN107287185B (zh) * | 2017-07-22 | 2021-05-18 | 贵州省园艺研究所 | 马铃薯四倍体栽培种与二倍体野生种原生质体融合方法 |
| CN113874388A (zh) | 2019-05-29 | 2021-12-31 | 主基因有限公司 | 孤雌生殖基因 |
| IL301700A (en) | 2020-10-13 | 2023-05-01 | Keygene Nv | Adapted promoter of parthenogenesis gene |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8200523A (nl) * | 1982-02-11 | 1983-09-01 | Univ Leiden | Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna. |
| SU1582990A3 (ru) * | 1983-01-17 | 1990-07-30 | Монсанто Компани (Фирма) | Способ получени трасформированных клеток двудольных растений |
| DE3416978A1 (de) * | 1983-05-12 | 1984-12-06 | Stauffer Chemical Co., Westport, Conn. | Durch liposom vermittelte transformation eukaryotischer zellen |
| EP0429093B1 (de) * | 1984-05-11 | 2001-08-08 | Syngenta Participations AG | Transformation von Pflanzenerbgut |
| ZA853553B (en) * | 1984-05-11 | 1985-12-24 | Ciba Geigy Ag | Transformation of hereditary material of plants |
| JPS6394929A (ja) * | 1986-06-23 | 1988-04-26 | バイオテクニカ・インタ−ナショナル・インコ−ポレ−テッド | 葉緑体の形質転換 |
-
1986
- 1986-11-19 FI FI864720A patent/FI864720A/fi not_active IP Right Cessation
- 1986-11-20 EP EP86116056A patent/EP0223247A3/de not_active Withdrawn
- 1986-11-20 GB GB8627520A patent/GB2183660B/en not_active Expired - Lifetime
- 1986-11-20 PT PT83781A patent/PT83781B/pt unknown
- 1986-11-20 IL IL80704A patent/IL80704A0/xx unknown
- 1986-11-21 CN CN198686107908A patent/CN86107908A/zh active Pending
- 1986-11-21 BR BR8605753A patent/BR8605753A/pt unknown
- 1986-11-21 NO NO864681A patent/NO864681L/no unknown
- 1986-11-21 ZA ZA868828A patent/ZA868828B/xx unknown
- 1986-11-21 PL PL1986262517A patent/PL262517A1/xx unknown
- 1986-11-21 HU HU864833A patent/HUT42672A/hu unknown
- 1986-11-21 AU AU65555/86A patent/AU6555586A/en not_active Abandoned
- 1986-11-21 DK DK559586A patent/DK559586A/da unknown
- 1986-11-22 JP JP61279546A patent/JPS62155093A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN86107908A (zh) | 1987-09-30 |
| GB2183660A (en) | 1987-06-10 |
| BR8605753A (pt) | 1987-08-25 |
| DK559586D0 (da) | 1986-11-21 |
| EP0223247A2 (de) | 1987-05-27 |
| FI864720A0 (fi) | 1986-11-19 |
| AU6555586A (en) | 1987-05-28 |
| GB2183660B (en) | 1990-01-10 |
| ZA868828B (en) | 1987-09-30 |
| EP0223247A3 (de) | 1989-11-02 |
| IL80704A0 (en) | 1987-02-27 |
| JPS62155093A (ja) | 1987-07-10 |
| NO864681D0 (no) | 1986-11-21 |
| GB8627520D0 (en) | 1986-12-17 |
| HUT42672A (en) | 1987-08-28 |
| PL262517A1 (en) | 1987-09-21 |
| PT83781A (en) | 1986-12-01 |
| DK559586A (da) | 1987-05-23 |
| FI864720A (fi) | 1987-05-23 |
| PT83781B (en) | 1988-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NO864681L (no) | Fremgangsmaate ved direkte genoverfoering i plastider og mitokondrier. | |
| JP7236121B2 (ja) | 植物のゲノム編集方法 | |
| Hess | Pollen-based techniques in genetic manipulation | |
| JP6967217B2 (ja) | 形質転換植物の作製方法 | |
| Li et al. | Optimizing Agrobacterium-mediated transformation of grapevine | |
| Rohini et al. | Embryo transformation, a practical approach for realizing transgenic plants of safflower (Carthamus tinctorius L.) | |
| ES2256856T3 (es) | Proceso de seccion de celulas transgenicas. | |
| JP2000078936A (ja) | 植物の遺伝物質の形質転換法 | |
| JP2000083680A (ja) | 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ― | |
| MXPA03010967A (es) | Cromosomas artificiales de plantas, usos de los mismos y metodos para preparar cromosomas artificiales de plantas. | |
| Sheikh Beig Goharrizi et al. | Agrobacterium mediated transformation of somatic embryos of Persian walnut using fld gene for osmotic stress tolerance | |
| US20240093222A1 (en) | Methods for transforming corn explants | |
| Sidorov et al. | Agrobacterium-mediated maize transformation: immature embryos versus callus | |
| Maliga | Plastid transformation in flowering plants | |
| MX2007002083A (es) | Metodos para regeneracion, transformacion y produccion de plantas de abelmoschus esculentus transgenica resistente a insectos. | |
| EP0737748A1 (en) | Efficient production of transgenic fertile homozygous plants from fresh microspores | |
| Dandekar et al. | Plant transformation: Agrobacterium-mediated gene transfer | |
| Soliman | Production of genetically modified grape (Vitis vinifera L.) plants | |
| WO2021137299A1 (ja) | 植物の改変方法 | |
| US20130065312A1 (en) | Method and composition for generating programmed cell death resistant algal cells | |
| AU729635B2 (en) | A method for producing the transformants of coffee plants and transgenic coffee plants | |
| Rahangdale et al. | Chapter 2: Gene Transfer Methods in Plants | |
| Islam et al. | Regeneration and Genetic Transformation of Tossa Jute ('Corchorus olitorius L.') | |
| Bohorova et al. | Laboratory protocols: CIMMYT Applied genetic engineering laboratory | |
| CN108359688A (zh) | 提高植物对赤霉素抑制剂敏感性的方法及其应用 |