NO332500B1 - Immunogene polypeptider, polynukleotider som koder for disse, farmasoytisk sammensetning, ekspresjonsvektor, vertscelle, vaksinesammensetning, anvendelse og fremgangsmate for fremstilling av polypeptidet - Google Patents
Immunogene polypeptider, polynukleotider som koder for disse, farmasoytisk sammensetning, ekspresjonsvektor, vertscelle, vaksinesammensetning, anvendelse og fremgangsmate for fremstilling av polypeptidet Download PDFInfo
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- NO332500B1 NO332500B1 NO20005050A NO20005050A NO332500B1 NO 332500 B1 NO332500 B1 NO 332500B1 NO 20005050 A NO20005050 A NO 20005050A NO 20005050 A NO20005050 A NO 20005050A NO 332500 B1 NO332500 B1 NO 332500B1
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- amino acid
- nucleotide sequence
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- acid sequence
- polypeptide
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
- A61P31/06—Antibacterial agents for tuberculosis
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
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- Organic Chemistry (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- Pulmonology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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| US09/056,556 US6350456B1 (en) | 1997-03-13 | 1998-04-07 | Compositions and methods for the prevention and treatment of M. tuberculosis infection |
| US09/223,040 US6544522B1 (en) | 1998-12-30 | 1998-12-30 | Fusion proteins of mycobacterium tuberculosis antigens and their uses |
| PCT/US1999/007717 WO1999051748A2 (en) | 1998-04-07 | 1999-04-07 | Fusion proteins of mycobacterium tuberculosis antigens and their uses |
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| NO20005050D0 NO20005050D0 (no) | 2000-10-06 |
| NO20005050L NO20005050L (no) | 2000-11-30 |
| NO332500B1 true NO332500B1 (no) | 2012-10-01 |
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| NO20005050A NO332500B1 (no) | 1998-04-07 | 2000-10-06 | Immunogene polypeptider, polynukleotider som koder for disse, farmasoytisk sammensetning, ekspresjonsvektor, vertscelle, vaksinesammensetning, anvendelse og fremgangsmate for fremstilling av polypeptidet |
| NO20120621A NO20120621L (no) | 1998-04-07 | 2012-05-25 | Fusjonsproteiner fra mycobacterium tuberkulose og deres anvendelser |
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| NO20120621A NO20120621L (no) | 1998-04-07 | 2012-05-25 | Fusjonsproteiner fra mycobacterium tuberkulose og deres anvendelser |
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| US6592877B1 (en) | 1995-09-01 | 2003-07-15 | Corixa Corporation | Compounds and methods for immunotherapy and diagnosis of tuberculosis |
| US6458366B1 (en) | 1995-09-01 | 2002-10-01 | Corixa Corporation | Compounds and methods for diagnosis of tuberculosis |
| US6290969B1 (en) * | 1995-09-01 | 2001-09-18 | Corixa Corporation | Compounds and methods for immunotherapy and diagnosis of tuberculosis |
| BR9909472A (pt) | 1998-04-07 | 2001-09-11 | Corixa Corp | Polipeptìdeo purificado, processo para prevenir tuberculose, e, composição farmacêutica |
| DE69941545D1 (de) * | 1998-11-04 | 2009-11-26 | Isis Innovation | Tuberkulose diagnose test |
| US6565856B1 (en) * | 1998-12-08 | 2003-05-20 | Corixa Corporation | Compounds and methods for treatment and diagnosis of chlamydial infection |
| US6432916B1 (en) | 1998-12-08 | 2002-08-13 | Corixa Corporation | Compounds and methods for treatment and diagnosis of chlamydial infection |
| US6448234B1 (en) | 1998-12-08 | 2002-09-10 | Corixa Corporation | Compounds and methods for treatment and diagnosis of chlamydial infection |
| US20020061848A1 (en) | 2000-07-20 | 2002-05-23 | Ajay Bhatia | Compounds and methods for treatment and diagnosis of chlamydial infection |
| US6555115B1 (en) | 1998-12-08 | 2003-04-29 | Corixa Corporation | Compounds and methods for treatment and diagnosis of chlamydial infection |
| US6447779B1 (en) | 1998-12-08 | 2002-09-10 | Corixa Corporation | Compounds for the diagnosis of Chlamydial infection |
| US20030027774A1 (en) * | 1999-03-18 | 2003-02-06 | Ronald C. Hendrickson | Tuberculosis antigens and methods of use therefor |
| US8143386B2 (en) * | 1999-04-07 | 2012-03-27 | Corixa Corporation | Fusion proteins of mycobacterium tuberculosis antigens and their uses |
| EP1517913B9 (en) * | 1999-10-07 | 2007-08-29 | Corixa Corporation | A mycobacterium tuberculosis coding sequence for exression of heterologous proteins |
| US7009042B1 (en) | 1999-10-07 | 2006-03-07 | Corixa Corporation | Methods of using a Mycobacterium tuberculosis coding sequence to facilitate stable and high yield expression of the heterologous proteins |
| EP1229931A4 (en) * | 1999-10-07 | 2003-05-28 | Corixa Corp | FUSION PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS |
| US6316205B1 (en) | 2000-01-28 | 2001-11-13 | Genelabs Diagnostics Pte Ltd. | Assay devices and methods of analyte detection |
| WO2001062893A2 (en) | 2000-02-25 | 2001-08-30 | Corixa Corporation | Compounds and methods for diagnosis and immunotherapy of tuberculosis |
| GB0006692D0 (en) * | 2000-03-20 | 2000-05-10 | Glaxo Group Ltd | Epitopes |
| US6919187B2 (en) | 2000-04-21 | 2005-07-19 | Corixa Corporation | Compounds and methods for treatment and diagnosis of chlamydial infection |
| AU2001255596A1 (en) | 2000-04-21 | 2001-11-07 | Corixa Corporation | Compounds and methods for treatment and diagnosis of chlamydial infection |
| PT2133100E (pt) | 2000-06-20 | 2012-01-11 | Corixa Corp | Antigénio mtb32a de mycobacterium tuberculosis com um local activo inactivado e suas proteínas de fusão |
| GB0109297D0 (en) | 2001-04-12 | 2001-05-30 | Glaxosmithkline Biolog Sa | Vaccine |
| US6841159B2 (en) | 2002-01-30 | 2005-01-11 | The United States Of America As Represented By The Secretary Of The Navy | Rapid lateral flow assay for determining exposure to Mycobacterium tuberculosis and other mycobacteria |
| AU2003213118A1 (en) | 2002-02-15 | 2003-09-09 | Corixa Corporation | Fusion proteins of mycobacterium tuberculosis |
| GB0321615D0 (en) | 2003-09-15 | 2003-10-15 | Glaxo Group Ltd | Improvements in vaccination |
| WO2005061534A2 (en) * | 2003-12-23 | 2005-07-07 | Statens Serum Institut | Improved tuberculosis vaccines |
| US20120027841A1 (en) | 2004-01-07 | 2012-02-02 | Biomedical Research Models, Inc. | Novel mucosal vaccination approach for herpes simplex virus type-2 |
| CA2580796C (en) | 2004-09-24 | 2013-03-26 | Amgen Inc. | Modified fc molecules having peptides inserted in internal loop regions |
| US8475735B2 (en) | 2004-11-01 | 2013-07-02 | Uma Mahesh Babu | Disposable immunodiagnostic test system |
| BRPI0518933A2 (pt) | 2004-11-16 | 2008-12-16 | Crucell Holland B V E Aeras Gl | adenovÍrus recombinante defeituoso de replicaÇço, vetor de polinucleotÍdeo recombinante, vacina multivalente da tuberculose, e, uso do antÍgeno tb10.4 de mycobacterium |
| CN100368437C (zh) * | 2005-01-28 | 2008-02-13 | 中国农业科学院哈尔滨兽医研究所 | 一种诊断牛结核病的重组抗原蛋白及其制备方法 |
| PT2426141E (pt) | 2005-04-29 | 2014-11-25 | Glaxosmithkline Biolog Sa | Método para prevenção ou tratamento de uma infecção por m. tuberculosis |
| US8008453B2 (en) | 2005-08-12 | 2011-08-30 | Amgen Inc. | Modified Fc molecules |
| TWI457133B (zh) | 2005-12-13 | 2014-10-21 | Glaxosmithkline Biolog Sa | 新穎組合物 |
| CN100999550B (zh) * | 2006-01-10 | 2010-10-06 | 中国人民解放军第三○九医院 | 结核分枝杆菌融合蛋白及其应用 |
| CN101311189B (zh) * | 2006-06-06 | 2010-09-08 | 中国人民解放军第二军医大学 | 激发人体抗结核杆菌的保护性免疫反应的抗原表位及其用途 |
| CN101298471B (zh) * | 2006-06-06 | 2011-01-19 | 中国人民解放军第二军医大学 | 激发人体抗结核杆菌的保护性免疫反应的抗原表位及其用途 |
| CN100415771C (zh) * | 2006-06-06 | 2008-09-03 | 中国人民解放军第二军医大学 | 激发人体抗结核杆菌的保护性免疫反应的抗原表位及其用途 |
| CN101311190B (zh) * | 2006-06-06 | 2011-08-03 | 中国人民解放军第二军医大学 | 激发人体抗结核杆菌的保护性免疫反应的抗原表位及其用途 |
| KR20090024292A (ko) * | 2006-06-28 | 2009-03-06 | 스태튼스 세룸 인스티튜트 | 단백질 단편 또는 펩타이드 혼합물로서 전달되는 항원을 가진 백신접종에 의해 준우성 항원결정기를 포함하는 ｔ세포 레퍼토리 확장 |
| US20090181078A1 (en) | 2006-09-26 | 2009-07-16 | Infectious Disease Research Institute | Vaccine composition containing synthetic adjuvant |
| EP2468300B1 (en) | 2006-09-26 | 2017-12-20 | Infectious Disease Research Institute | Vaccine composition containing synthetic adjuvant |
| CA2664619C (en) | 2006-10-12 | 2012-12-11 | Glaxosmithkline Biologicals S.A. | Immunogenic compositions comprising an oil-in-water emulsion adjuvant containing a reduced amount of squalene, tocol and an emulsifying agent |
| EP2433648A3 (en) | 2006-10-12 | 2012-04-04 | GlaxoSmithKline Biologicals S.A. | Vaccine comprising an oil in water emulsion adjuvant |
| US9717788B2 (en) | 2007-03-02 | 2017-08-01 | Glaxosmithkline Biologicals Sa | Method of inducing an immune response against HIV employing HIV immunogens, adenoviral vectors encoding said immunogens, and adjuvant |
| CN103550764A (zh) | 2007-03-02 | 2014-02-05 | 葛兰素史密丝克莱恩生物有限公司 | 疫苗组合物及其在刺激免疫反应中的用途 |
| WO2008124647A2 (en) | 2007-04-04 | 2008-10-16 | Infectious Disease Research Institute | Immunogenic compositions comprising mycobacterium tuberculosis polypeptides and fusions thereof |
| RU2489165C2 (ru) * | 2007-10-13 | 2013-08-10 | Корнел Унивесити | ФАРМАЦЕВТИЧЕСКАЯ КОМПОЗИЦИЯ И СПОСОБ СТИМУЛИРОВАНИЯ ИММУННОГО ОТВЕТА К Мусоbacterium avium ПОДВИДА Paratuberculosis |
| KR101378240B1 (ko) | 2008-02-22 | 2014-03-28 | 포항공과대학교 산학협력단 | 결핵 예방용 조성물 |
| NO2315597T3 (enExample) | 2008-07-25 | 2018-01-20 | ||
| HRP20181353T1 (hr) | 2008-07-25 | 2018-10-19 | Glaxosmithkline Biologicals S.A. | Tuberkulozni protein rv2386c, njegovi pripravci i upotreba |
| EP2315773B1 (en) * | 2008-07-25 | 2016-08-24 | GlaxoSmithKline Biologicals S.A. | Polypeptides, polynucleotides and compositions for use in the treatment of latent tubeculosis |
| GB0815872D0 (en) | 2008-09-01 | 2008-10-08 | Pasteur Institut | Novel method and compositions |
| CN108373509A (zh) | 2009-03-10 | 2018-08-07 | 贝勒研究院 | 靶向抗原呈递细胞的抗病毒疫苗 |
| BRPI1009454A2 (pt) * | 2009-03-10 | 2019-09-24 | Baylor Res Institute | vacinas contra o câncer direcionadas a células apresentadoras de antígenos |
| BRPI1009455A2 (pt) | 2009-03-10 | 2016-03-01 | Baylor Res Inst | anticorpos anti-c40 e usos dos mesmos |
| CA2764374C (en) | 2009-06-05 | 2019-11-19 | Infectious Disease Research Institute | Synthetic glucopyranosyl lipid adjuvants |
| LT2528621T (lt) | 2010-01-27 | 2016-12-12 | Glaxosmithkline Biologicals S.A. | Modifikuoti tuberkuliozės antigenai |
| SG190937A1 (en) | 2010-12-14 | 2013-07-31 | Glaxosmithkline Biolog Sa | Mycobacterium antigenic composition |
| GB201022007D0 (en) | 2010-12-24 | 2011-02-02 | Imp Innovations Ltd | DNA-sensor |
| CN102154324B (zh) * | 2010-12-29 | 2012-11-28 | 兰州大学 | 结核分枝杆菌融合蛋白Mtb10.4-Hsp16.3的构建、表达和纯化方法及其应用 |
| US20120301465A1 (en) | 2011-03-25 | 2012-11-29 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Compositions and methods to immunize against hepatitis c virus |
| NZ616304A (en) | 2011-04-08 | 2016-01-29 | Immune Design Corp | Immunogenic compositions and methods of using the compositions for inducing humoral and cellular immune responses |
| US20120288515A1 (en) | 2011-04-27 | 2012-11-15 | Immune Design Corp. | Synthetic long peptide (slp)-based vaccines |
| CN102305855A (zh) * | 2011-05-19 | 2012-01-04 | 中山大学 | 体外检测结核分枝杆菌感染的试剂和方法 |
| EP2771469A4 (en) * | 2011-10-24 | 2015-04-08 | Univ Central Florida Res Found | CONCENTRATED PLASTIC-EXPRESSED MYCOBACTERIUM TUBERCULOSE VACCINE FUEL ESTATES ESAT-6 AND MTB72F AT CHOLERATOXIN B SUB-UNIT |
| PL2811981T3 (pl) | 2012-02-07 | 2019-09-30 | Infectious Disease Research Institute | Ulepszone formulacje adiuwantowe zawierające agonistę TLR4 oraz sposoby ich zastosowania |
| MX347154B (es) | 2012-02-24 | 2017-02-21 | Centro De Investigación Científica Y De Educación Superior De Ensenada Baja California (Cicese) | Prueba de diagnóstico para enfermedades infecciosas en ganado bovino. |
| SMT201800368T1 (it) | 2012-05-16 | 2018-09-13 | Immune Design Corp | Vaccini per l'infezione da virus-2 herpes simplex |
| CN102757971B (zh) * | 2012-08-07 | 2013-09-11 | 中国人民解放军第三O九医院 | 一种结核分枝杆菌重组蛋白质及其制备方法 |
| EP2912056B1 (en) | 2012-10-23 | 2020-02-12 | Statens Serum Institut | M. tuberculosis vaccines |
| EP2941269B1 (en) | 2013-01-07 | 2020-12-09 | Mucosal Vaccine Technologies LLC | Therapeutic vaccines for treating herpes simplex virus type 2 infections |
| CN105025921B (zh) * | 2013-03-15 | 2019-02-19 | 宾夕法尼亚大学理事会 | 用于结核病的预防或治疗的合成免疫原 |
| CA2909221A1 (en) | 2013-04-18 | 2014-10-23 | Immune Design Corp. | Gla monotherapy for use in cancer treatment |
| US9463198B2 (en) | 2013-06-04 | 2016-10-11 | Infectious Disease Research Institute | Compositions and methods for reducing or preventing metastasis |
| IL310015B2 (en) | 2013-12-31 | 2026-02-01 | Access To Advanced Health Inst | Single vial formulation |
| WO2015106281A1 (en) | 2014-01-13 | 2015-07-16 | Baylor Research Institute | Novel vaccines against hpv and hpv-related diseases |
| CA3014458A1 (en) * | 2016-03-02 | 2017-09-08 | Cue Biopharma, Inc. | T-cell modulatory multimeric polypeptides and methods of use thereof |
| WO2017176076A1 (en) | 2016-04-06 | 2017-10-12 | Ewha University - Industry Collaboration Foundation | A peptide with ability to penetrate cell membrane |
| KR102483033B1 (ko) | 2016-05-16 | 2022-12-30 | 액세스 투 어드밴스드 헬스 인스티튜트 | 페길화된 리포솜 및 이의 용도 |
| RU2761870C2 (ru) | 2016-05-16 | 2021-12-13 | Инфекшес Дизис Рисёрч Инститьют | Состав, содержащий агонист tlr, и способы применения |
| EP3463300A1 (en) | 2016-06-01 | 2019-04-10 | Infectious Disease Research Institute | Nanoalum particles containing a sizing agent |
| AU2018285694B2 (en) | 2017-06-15 | 2025-04-10 | Access To Advanced Health Institute | Nanostructured lipid carriers and stable emulsions and uses thereof |
| EP3678695A1 (en) | 2017-09-08 | 2020-07-15 | Infectious Disease Research Institute | Liposomal formulations comprising saponin and methods of use |
| US10610585B2 (en) | 2017-09-26 | 2020-04-07 | Inserm (Institut National De La Sante Et De La Recherche Medicale) | Methods and compositions for treating and preventing HIV |
| CN109825497A (zh) * | 2019-01-25 | 2019-05-31 | 石河子大学 | 一种新型结核杆菌融合菌株的制备方法及其应用 |
| BR112021023726A2 (pt) | 2019-05-25 | 2022-02-15 | Infectious Disease Res Inst | Composição e método para secagem por pulverização de uma emulsão de vacina adjuvante |
| WO2024052882A1 (en) | 2022-09-09 | 2024-03-14 | Access To Advanced Health Institute | Immunogenic vaccine composition incorporating a saponin |
| CN118652311B (zh) * | 2024-07-17 | 2025-02-25 | 扬州大学 | 结核分枝杆菌复合体ⅶ型分泌系统效应蛋白cfp10保守功能性b细胞表位及其应用 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4980286A (en) | 1985-07-05 | 1990-12-25 | Whitehead Institute For Biomedical Research | In vivo introduction and expression of foreign genetic material in epithelial cells |
| US5075109A (en) | 1986-10-24 | 1991-12-24 | Southern Research Institute | Method of potentiating an immune response |
| US6024983A (en) | 1986-10-24 | 2000-02-15 | Southern Research Institute | Composition for delivering bioactive agents for immune response and its preparation |
| US4897268A (en) | 1987-08-03 | 1990-01-30 | Southern Research Institute | Drug delivery system and method of making the same |
| US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
| WO1990013361A1 (en) | 1989-05-04 | 1990-11-15 | Southern Research Institute | Improved encapsulation process and products therefrom |
| AU660629B2 (en) | 1990-10-01 | 1995-07-06 | University Of Connecticut, The | Targeting viruses and cells for selective internalization by cells |
| WO1992020316A2 (en) | 1991-05-14 | 1992-11-26 | University Of Connecticut | Targeted delivery of genes encoding immunogenic proteins |
| DK0587738T3 (da) | 1991-06-05 | 2000-12-18 | Univ Connecticut | Destineret levering af gener, som koder for sekretionsproteiner |
| DK0528306T3 (da) * | 1991-08-15 | 2000-04-25 | Hoffmann La Roche | Mycobacterium-primerpar |
| WO1993014188A1 (en) | 1992-01-17 | 1993-07-22 | The Regents Of The University Of Michigan | Targeted virus |
| AU3940293A (en) | 1992-04-03 | 1993-11-08 | Alexander T. YOUNG | Gene therapy using targeted viral vectors |
| DE69313134T2 (de) | 1992-06-25 | 1998-02-26 | Smithkline Beecham Biolog | Adjuvantien enthaltende impfstoffzusammensetzung |
| US5330754A (en) * | 1992-06-29 | 1994-07-19 | Archana Kapoor | Membrane-associated immunogens of mycobacteria |
| US5359681A (en) | 1993-01-11 | 1994-10-25 | University Of Washington | Fiber optic sensor and methods and apparatus relating thereto |
| US5955077A (en) | 1993-07-02 | 1999-09-21 | Statens Seruminstitut | Tuberculosis vaccine |
| DK79793D0 (da) * | 1993-07-02 | 1993-07-02 | Statens Seruminstitut | Diagnostic test |
| DK79893D0 (da) * | 1993-07-02 | 1993-07-02 | Statens Seruminstitut | New vaccine |
| US5679647A (en) | 1993-08-26 | 1997-10-21 | The Regents Of The University Of California | Methods and devices for immunizing a host against tumor-associated antigens through administration of naked polynucleotides which encode tumor-associated antigenic peptides |
| GB9326253D0 (en) | 1993-12-23 | 1994-02-23 | Smithkline Beecham Biolog | Vaccines |
| UA56132C2 (uk) | 1995-04-25 | 2003-05-15 | Смітклайн Бічем Байолоджікалс С.А. | Композиція вакцини (варіанти), спосіб стабілізації qs21 відносно гідролізу (варіанти), спосіб приготування композиції вакцини |
| MX9801687A (es) | 1995-09-01 | 1998-11-29 | Corixa Corp | Compuestos y metodos para el diagnostico de tuberculosis. |
| EP1203817B9 (en) * | 1995-09-01 | 2006-08-16 | Corixa Corporation | Compounds and methods for immunotherapy and diagnosis of tuberculosis |
| US6544522B1 (en) * | 1998-12-30 | 2003-04-08 | Corixa Corporation | Fusion proteins of mycobacterium tuberculosis antigens and their uses |
| US6627198B2 (en) * | 1997-03-13 | 2003-09-30 | Corixa Corporation | Fusion proteins of Mycobacterium tuberculosis antigens and their uses |
| BR9909472A (pt) | 1998-04-07 | 2001-09-11 | Corixa Corp | Polipeptìdeo purificado, processo para prevenir tuberculose, e, composição farmacêutica |
-
1999
- 1999-04-07 BR BR9909472-0A patent/BR9909472A/pt not_active Application Discontinuation
- 1999-04-07 JP JP2000542460A patent/JP4227302B2/ja not_active Expired - Fee Related
- 1999-04-07 TR TR2000/02938T patent/TR200002938T2/xx unknown
- 1999-04-07 NZ NZ507378A patent/NZ507378A/xx unknown
- 1999-04-07 CN CNB998070777A patent/CN1163602C/zh not_active Expired - Fee Related
- 1999-04-07 CN CN2004100618462A patent/CN1629185B/zh not_active Expired - Fee Related
- 1999-04-07 WO PCT/US1999/007717 patent/WO1999051748A2/en not_active Ceased
- 1999-04-07 CA CA2326598A patent/CA2326598C/en not_active Expired - Fee Related
- 1999-04-07 CZ CZ20003652A patent/CZ302870B6/cs not_active IP Right Cessation
- 1999-04-07 AU AU34817/99A patent/AU753995B2/en not_active Ceased
- 1999-04-07 IL IL13880999A patent/IL138809A0/xx unknown
- 1999-04-07 KR KR1020007011196A patent/KR100692227B1/ko not_active Expired - Fee Related
- 1999-04-07 PL PL344142A patent/PL202844B1/pl not_active IP Right Cessation
- 1999-04-07 HU HU0101521A patent/HU228354B1/hu not_active IP Right Cessation
- 1999-04-07 KR KR1020067009456A patent/KR100797876B1/ko not_active Expired - Fee Related
- 1999-04-07 EP EP99916513A patent/EP1068329A2/en not_active Withdrawn
- 1999-08-17 SA SA99200488A patent/SA99200488B1/ar unknown
-
2000
- 2000-06-20 US US09/597,796 patent/US7186412B1/en not_active Expired - Fee Related
- 2000-10-06 NO NO20005050A patent/NO332500B1/no not_active IP Right Cessation
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2006
- 2006-03-30 US US11/396,117 patent/US7261897B2/en not_active Expired - Fee Related
- 2006-03-31 JP JP2006100968A patent/JP4326008B2/ja not_active Expired - Fee Related
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2012
- 2012-05-25 NO NO20120621A patent/NO20120621L/no not_active Application Discontinuation
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