NO323174B1 - Immortalisert patterdyrcellelinje og fremgangsmate for immortalisering og dissimortalisering av celler - Google Patents

Immortalisert patterdyrcellelinje og fremgangsmate for immortalisering og dissimortalisering av celler Download PDF

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Publication number
NO323174B1
NO323174B1 NO19975557A NO975557A NO323174B1 NO 323174 B1 NO323174 B1 NO 323174B1 NO 19975557 A NO19975557 A NO 19975557A NO 975557 A NO975557 A NO 975557A NO 323174 B1 NO323174 B1 NO 323174B1
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Prior art keywords
gene
cells
immortalization
selection marker
nucleic acid
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NO19975557A
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English (en)
Norwegian (no)
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NO975557L (no
NO975557D0 (no
Inventor
David J Anderson
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California Inst Of Techn
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Application filed by California Inst Of Techn filed Critical California Inst Of Techn
Publication of NO975557D0 publication Critical patent/NO975557D0/no
Publication of NO975557L publication Critical patent/NO975557L/no
Publication of NO323174B1 publication Critical patent/NO323174B1/no

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/162Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/10041Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/13011Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
NO19975557A 1995-06-07 1997-12-02 Immortalisert patterdyrcellelinje og fremgangsmate for immortalisering og dissimortalisering av celler NO323174B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/481,127 US5629159A (en) 1995-06-07 1995-06-07 Immortalization and disimmortalization of cells
PCT/US1996/009781 WO1996040877A1 (en) 1995-06-07 1996-06-06 Immortalization and disimmortalization of cells

Publications (3)

Publication Number Publication Date
NO975557D0 NO975557D0 (no) 1997-12-02
NO975557L NO975557L (no) 1998-02-05
NO323174B1 true NO323174B1 (no) 2007-01-15

Family

ID=23910719

Family Applications (1)

Application Number Title Priority Date Filing Date
NO19975557A NO323174B1 (no) 1995-06-07 1997-12-02 Immortalisert patterdyrcellelinje og fremgangsmate for immortalisering og dissimortalisering av celler

Country Status (10)

Country Link
US (1) US5629159A (es)
EP (1) EP0832196B1 (es)
JP (1) JP3875267B2 (es)
AT (1) ATE255632T1 (es)
AU (1) AU700690B2 (es)
CA (1) CA2222425C (es)
DE (1) DE69630955T2 (es)
ES (1) ES2211959T3 (es)
NO (1) NO323174B1 (es)
WO (1) WO1996040877A1 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110743A (en) * 1995-02-10 2000-08-29 The Regents Of The University Of California Development and use of human pancreatic cell lines
US20030202967A1 (en) * 1995-02-10 2003-10-30 The Regents Of The University Of California Development and use of human pancreatic cell lines
FR2737731B1 (fr) * 1995-08-07 1997-10-10 Pasteur Institut Sequence de retroelements naturels ou synthetiques ayant pour fonction de permettre l'insertion de sequences nucleotidiques dans une cellule eucaryote
US6743620B1 (en) * 1996-12-16 2004-06-01 Eisai Co., Ltd. Method for preparing retrovirus vector for gene therapy
AU736267B2 (en) * 1996-12-31 2001-07-26 Omeros Corporation Vectors and methods for the mutagenesis of mammalian genes
DE19740571C1 (de) * 1997-09-15 1999-03-18 Gsf Forschungszentrum Umwelt Verfahren zur Stimulation von T-Zellen mit gewünschter Antigenspezifität
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
JP2001520877A (ja) * 1997-10-28 2001-11-06 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 血管平滑筋細胞のインビトロ分化、それに関する方法および試薬
DE19816116C2 (de) * 1998-04-09 2000-06-29 Gsf Forschungszentrum Umwelt Verfahren zur Immortalisation von Zellen mit Hilfe konditional transformierter Helferzellen
AU6162199A (en) * 1998-09-25 2000-04-17 Harvard-Mit Division Of Health Science And Technology Reversibly immortalized hepatocytes and methods of use
NZ513598A (en) 1999-01-21 2001-09-28 Vitro Diagnostics Inc Immortalized cell lines and methods of making the same
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
EP1103615A1 (en) * 1999-11-25 2001-05-30 Universite De Geneve Vectors capable of immortalizing non-dividing cells and cells immortalized with said vectors
US20040231006A1 (en) * 2000-04-12 2004-11-18 Silver Daniel P. Self-extinguishing recombinases, nucleic acids encoding them and methods of using the same
ATE427994T1 (de) 2000-04-12 2009-04-15 Dana Farber Cancer Inst Inc Selbstlíschende rekombinasen, dafur kodierende nukleinsauren und verfahren zu deren verwendung
FR2810046B1 (fr) * 2000-06-07 2004-09-10 Neurotech Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre
JP4185264B2 (ja) * 2001-07-02 2008-11-26 富士フイルム株式会社 偏光方向制御素子及び露光装置
JP2003033179A (ja) * 2001-07-05 2003-02-04 Asahi Kasei Corp 可逆的遺伝子導入ベクター
US6645763B2 (en) * 2001-10-12 2003-11-11 Naoya Kobayashi Immortalized bone marrow mesenchymal stem cell
GB0300208D0 (en) * 2003-01-06 2003-02-05 Oxford Biomedica Ltd Insulin producing cells
US7485457B2 (en) * 2003-04-17 2009-02-03 United States Of America Department Of Veterans Affairs Isolated/cloned human NT2 cell lines expressing serotonin and GABA
GB0316882D0 (en) 2003-07-18 2003-08-20 Consejo Superior Investigacion Reversible immortalization of OEG from human olfactory bulbs as a tool to promote spinal cord regeneration
CA2584738A1 (en) * 2004-10-20 2006-04-27 Vitro Diagnostics, Inc. Generation and differentiation of adult stem cell lines
US20070093439A1 (en) * 2005-10-25 2007-04-26 Idera Pharmaceuticals, Inc. Short immunomodulatory oligonucleotides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1293460C (en) * 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5399346A (en) * 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
WO1992015694A1 (en) * 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
CA2140884A1 (en) * 1992-07-27 1994-02-03 David J. Anderson Mammalian multipotent neural stem cells
US5387237A (en) * 1992-07-30 1995-02-07 The University Of Toledo Bioartificial pancreas

Also Published As

Publication number Publication date
EP0832196B1 (en) 2003-12-03
US5629159A (en) 1997-05-13
AU6107796A (en) 1996-12-30
ATE255632T1 (de) 2003-12-15
WO1996040877A1 (en) 1996-12-19
CA2222425C (en) 2007-08-21
ES2211959T3 (es) 2004-07-16
AU700690B2 (en) 1999-01-14
DE69630955D1 (de) 2004-01-15
DE69630955T2 (de) 2004-10-21
CA2222425A1 (en) 1996-12-19
NO975557L (no) 1998-02-05
EP0832196A1 (en) 1998-04-01
JPH11507230A (ja) 1999-06-29
NO975557D0 (no) 1997-12-02
JP3875267B2 (ja) 2007-01-31

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