FR2810046B1 - Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre - Google Patents

Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre

Info

Publication number
FR2810046B1
FR2810046B1 FR0007307A FR0007307A FR2810046B1 FR 2810046 B1 FR2810046 B1 FR 2810046B1 FR 0007307 A FR0007307 A FR 0007307A FR 0007307 A FR0007307 A FR 0007307A FR 2810046 B1 FR2810046 B1 FR 2810046B1
Authority
FR
France
Prior art keywords
cells
implementation
protein constructions
conditional immortalization
immortalization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR0007307A
Other languages
English (en)
Other versions
FR2810046A1 (fr
Inventor
Eric Parmentier
Florence Martin
Joel Crouzet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NEUROTECH
Original Assignee
NEUROTECH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NEUROTECH filed Critical NEUROTECH
Priority to FR0007307A priority Critical patent/FR2810046B1/fr
Priority to PCT/FR2001/001767 priority patent/WO2001094556A1/fr
Priority to AU2001267612A priority patent/AU2001267612A1/en
Publication of FR2810046A1 publication Critical patent/FR2810046A1/fr
Application granted granted Critical
Publication of FR2810046B1 publication Critical patent/FR2810046B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FR0007307A 2000-06-07 2000-06-07 Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre Expired - Fee Related FR2810046B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR0007307A FR2810046B1 (fr) 2000-06-07 2000-06-07 Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre
PCT/FR2001/001767 WO2001094556A1 (fr) 2000-06-07 2001-06-07 Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre
AU2001267612A AU2001267612A1 (en) 2000-06-07 2001-06-07 Method for conditionally immortalising cells and protein constructs therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0007307A FR2810046B1 (fr) 2000-06-07 2000-06-07 Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre

Publications (2)

Publication Number Publication Date
FR2810046A1 FR2810046A1 (fr) 2001-12-14
FR2810046B1 true FR2810046B1 (fr) 2004-09-10

Family

ID=8851060

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0007307A Expired - Fee Related FR2810046B1 (fr) 2000-06-07 2000-06-07 Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre

Country Status (3)

Country Link
AU (1) AU2001267612A1 (fr)
FR (1) FR2810046B1 (fr)
WO (1) WO2001094556A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014202016B2 (en) * 2005-10-18 2016-05-19 National Jewish Health Conditionally immortalized long-term stem cells and methods of making and using such cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2662698A1 (fr) * 1990-06-05 1991-12-06 Centre Nat Rech Scient Nouveaux facteurs de croissance neurotropes comprenant un peptide homeoboite.
US5811281A (en) * 1993-07-12 1998-09-22 Cornell Research Foundation, Inc. Immortalized intestinal epithelial cell lines
US5629159A (en) * 1995-06-07 1997-05-13 California Institute Of Technology Immortalization and disimmortalization of cells
DE19740571C1 (de) * 1997-09-15 1999-03-18 Gsf Forschungszentrum Umwelt Verfahren zur Stimulation von T-Zellen mit gewünschter Antigenspezifität
CA2315265A1 (fr) * 1998-01-12 1999-07-15 Cold Spring Harbor Laboratory Allongement de la duree de vie d'une cellule, et procedes et reactifs associes
JP2002541786A (ja) * 1999-04-12 2002-12-10 モデックス テラピューティク ソシエテ アノニム 遺伝子治療における使用のための一過性不死化細胞

Also Published As

Publication number Publication date
FR2810046A1 (fr) 2001-12-14
WO2001094556A1 (fr) 2001-12-13
AU2001267612A1 (en) 2001-12-17

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Legal Events

Date Code Title Description
ST Notification of lapse