NO322133B1 - Fremgangsmate for fremstilling av rensede viruslignende partikler (VLP-er) av humant papillomavirus. - Google Patents
Fremgangsmate for fremstilling av rensede viruslignende partikler (VLP-er) av humant papillomavirus. Download PDFInfo
- Publication number
- NO322133B1 NO322133B1 NO19964862A NO964862A NO322133B1 NO 322133 B1 NO322133 B1 NO 322133B1 NO 19964862 A NO19964862 A NO 19964862A NO 964862 A NO964862 A NO 964862A NO 322133 B1 NO322133 B1 NO 322133B1
- Authority
- NO
- Norway
- Prior art keywords
- protein
- yeast
- crpv
- hpv
- gene
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000002245 particle Substances 0.000 title claims abstract description 11
- 241000701806 Human papillomavirus Species 0.000 title claims description 61
- 238000002360 preparation method Methods 0.000 title description 19
- 230000008569 process Effects 0.000 title description 7
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 143
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 127
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 8
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 93
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 71
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 71
- 108020004414 DNA Proteins 0.000 claims description 40
- 238000004519 manufacturing process Methods 0.000 claims description 19
- 238000001542 size-exclusion chromatography Methods 0.000 claims description 8
- 102000053602 DNA Human genes 0.000 claims description 6
- 210000005253 yeast cell Anatomy 0.000 claims description 3
- 238000004255 ion exchange chromatography Methods 0.000 claims description 2
- 230000002269 spontaneous effect Effects 0.000 claims description 2
- 241001631646 Papillomaviridae Species 0.000 abstract description 41
- 239000013604 expression vector Substances 0.000 abstract description 18
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 112
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 85
- 239000013612 plasmid Substances 0.000 description 65
- 239000000872 buffer Substances 0.000 description 57
- 241000701646 Kappapapillomavirus 2 Species 0.000 description 47
- 239000011780 sodium chloride Substances 0.000 description 43
- 239000002609 medium Substances 0.000 description 42
- 239000012634 fragment Substances 0.000 description 38
- 239000008188 pellet Substances 0.000 description 37
- 239000000523 sample Substances 0.000 description 37
- 239000013598 vector Substances 0.000 description 35
- 239000006228 supernatant Substances 0.000 description 32
- 239000000499 gel Substances 0.000 description 30
- 238000001262 western blot Methods 0.000 description 29
- 239000000427 antigen Substances 0.000 description 28
- 108091007433 antigens Proteins 0.000 description 28
- 102000036639 antigens Human genes 0.000 description 28
- 238000001514 detection method Methods 0.000 description 28
- 238000003752 polymerase chain reaction Methods 0.000 description 28
- 238000005119 centrifugation Methods 0.000 description 27
- 230000014509 gene expression Effects 0.000 description 25
- 210000004379 membrane Anatomy 0.000 description 25
- 239000012528 membrane Substances 0.000 description 25
- 108090000565 Capsid Proteins Proteins 0.000 description 24
- 102100023321 Ceruloplasmin Human genes 0.000 description 24
- 101150050575 URA3 gene Proteins 0.000 description 24
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 24
- 101150009006 HIS3 gene Proteins 0.000 description 21
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 20
- 238000001493 electron microscopy Methods 0.000 description 20
- 239000000463 material Substances 0.000 description 20
- 239000001488 sodium phosphate Substances 0.000 description 20
- 229910000162 sodium phosphate Inorganic materials 0.000 description 20
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 20
- 238000004458 analytical method Methods 0.000 description 19
- 238000000746 purification Methods 0.000 description 19
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 19
- 229960005486 vaccine Drugs 0.000 description 19
- 239000012467 final product Substances 0.000 description 18
- 239000011347 resin Substances 0.000 description 18
- 229920005989 resin Polymers 0.000 description 18
- 101900163635 Cottontail rabbit papillomavirus Major capsid protein L1 Proteins 0.000 description 17
- 241000341655 Human papillomavirus type 16 Species 0.000 description 17
- 241000283973 Oryctolagus cuniculus Species 0.000 description 17
- 239000002953 phosphate buffered saline Substances 0.000 description 17
- 239000013615 primer Substances 0.000 description 17
- 238000010276 construction Methods 0.000 description 16
- 239000000243 solution Substances 0.000 description 16
- 101900043895 Cottontail rabbit papillomavirus Minor capsid protein L2 Proteins 0.000 description 15
- 101150075484 MNN9 gene Proteins 0.000 description 15
- 241001465754 Metazoa Species 0.000 description 15
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 15
- 101150029183 PEP4 gene Proteins 0.000 description 14
- 230000003472 neutralizing effect Effects 0.000 description 14
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 13
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 13
- 101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 13
- 108010091212 pepstatin Proteins 0.000 description 13
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 208000002109 Argyria Diseases 0.000 description 12
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 12
- 241000700605 Viruses Species 0.000 description 12
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 12
- 239000006166 lysate Substances 0.000 description 12
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 12
- 229920001817 Agar Polymers 0.000 description 11
- 101100209954 Human papillomavirus type 16 L1 gene Proteins 0.000 description 11
- 239000008272 agar Substances 0.000 description 11
- 150000001413 amino acids Chemical group 0.000 description 11
- 238000000855 fermentation Methods 0.000 description 11
- 230000004151 fermentation Effects 0.000 description 11
- 229930182830 galactose Natural products 0.000 description 11
- 238000003119 immunoblot Methods 0.000 description 11
- 238000011534 incubation Methods 0.000 description 11
- 208000015181 infectious disease Diseases 0.000 description 11
- 239000000203 mixture Substances 0.000 description 11
- 210000002966 serum Anatomy 0.000 description 11
- 238000013518 transcription Methods 0.000 description 11
- 230000035897 transcription Effects 0.000 description 11
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 10
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 10
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 10
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 10
- 229910019142 PO4 Inorganic materials 0.000 description 10
- 239000012506 Sephacryl® Substances 0.000 description 10
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 10
- 229940024606 amino acid Drugs 0.000 description 10
- 235000001014 amino acid Nutrition 0.000 description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 10
- 235000011130 ammonium sulphate Nutrition 0.000 description 10
- 230000001413 cellular effect Effects 0.000 description 10
- 238000010828 elution Methods 0.000 description 10
- 239000008103 glucose Substances 0.000 description 10
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 239000002002 slurry Substances 0.000 description 10
- 229940035893 uracil Drugs 0.000 description 10
- 101150038242 GAL10 gene Proteins 0.000 description 9
- 102100024637 Galectin-10 Human genes 0.000 description 9
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 9
- 101150027802 L2 gene Proteins 0.000 description 9
- 101150101314 PRB1 gene Proteins 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 208000000260 Warts Diseases 0.000 description 9
- 239000006285 cell suspension Substances 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 239000012530 fluid Substances 0.000 description 9
- 239000010452 phosphate Substances 0.000 description 9
- 238000000926 separation method Methods 0.000 description 9
- 201000010153 skin papilloma Diseases 0.000 description 9
- 238000003756 stirring Methods 0.000 description 9
- SEHFUALWMUWDKS-UHFFFAOYSA-N 5-fluoroorotic acid Chemical compound OC(=O)C=1NC(=O)NC(=O)C=1F SEHFUALWMUWDKS-UHFFFAOYSA-N 0.000 description 8
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 8
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 8
- 238000002105 Southern blotting Methods 0.000 description 8
- 229930006000 Sucrose Natural products 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
- 230000004186 co-expression Effects 0.000 description 8
- 239000012149 elution buffer Substances 0.000 description 8
- 239000013613 expression plasmid Substances 0.000 description 8
- 238000009472 formulation Methods 0.000 description 8
- 108020001507 fusion proteins Proteins 0.000 description 8
- 102000037865 fusion proteins Human genes 0.000 description 8
- 230000002163 immunogen Effects 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- 238000010186 staining Methods 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- 230000009466 transformation Effects 0.000 description 8
- 238000009010 Bradford assay Methods 0.000 description 7
- 108020004705 Codon Proteins 0.000 description 7
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 238000002347 injection Methods 0.000 description 7
- 239000007924 injection Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 229930182817 methionine Natural products 0.000 description 7
- 229960004452 methionine Drugs 0.000 description 7
- IYDGMDWEHDFVQI-UHFFFAOYSA-N phosphoric acid;trioxotungsten Chemical compound O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O IYDGMDWEHDFVQI-UHFFFAOYSA-N 0.000 description 7
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 7
- AXAVXPMQTGXXJZ-UHFFFAOYSA-N 2-aminoacetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound NCC(O)=O.OCC(N)(CO)CO AXAVXPMQTGXXJZ-UHFFFAOYSA-N 0.000 description 6
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 6
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 6
- 108091026890 Coding region Proteins 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 6
- 101100049401 Human papillomavirus type 16 L2 gene Proteins 0.000 description 6
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 6
- 239000007993 MOPS buffer Substances 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- 108010001244 Tli polymerase Proteins 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 238000005194 fractionation Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 229960002885 histidine Drugs 0.000 description 6
- 229960003136 leucine Drugs 0.000 description 6
- 239000013642 negative control Substances 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 6
- 229920000053 polysorbate 80 Polymers 0.000 description 6
- 241000701447 unidentified baculovirus Species 0.000 description 6
- 238000011144 upstream manufacturing Methods 0.000 description 6
- 229930024421 Adenine Natural products 0.000 description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 5
- 206010059313 Anogenital warts Diseases 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 241000282412 Homo Species 0.000 description 5
- 101150075239 L1 gene Proteins 0.000 description 5
- 101100243377 Mus musculus Pepd gene Proteins 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 239000002033 PVDF binder Substances 0.000 description 5
- 208000009608 Papillomavirus Infections Diseases 0.000 description 5
- 238000012300 Sequence Analysis Methods 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 229960000643 adenine Drugs 0.000 description 5
- 238000013019 agitation Methods 0.000 description 5
- 238000005349 anion exchange Methods 0.000 description 5
- 230000005540 biological transmission Effects 0.000 description 5
- 238000005251 capillar electrophoresis Methods 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 239000013592 cell lysate Substances 0.000 description 5
- 229910052802 copper Inorganic materials 0.000 description 5
- 239000010949 copper Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000007717 exclusion Effects 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 238000009396 hybridization Methods 0.000 description 5
- 230000000977 initiatory effect Effects 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 5
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 5
- 238000000386 microscopy Methods 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 229960002566 papillomavirus vaccine Drugs 0.000 description 5
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 230000003612 virological effect Effects 0.000 description 5
- 241000283707 Capra Species 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 101001125486 Homo sapiens Basic salivary proline-rich protein 1 Proteins 0.000 description 4
- 101000742340 Human papillomavirus type 16 Minor capsid protein L2 Proteins 0.000 description 4
- 206010061598 Immunodeficiency Diseases 0.000 description 4
- 101150034230 LI gene Proteins 0.000 description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 108700026244 Open Reading Frames Proteins 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 4
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 4
- 238000005571 anion exchange chromatography Methods 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 230000003111 delayed effect Effects 0.000 description 4
- 238000007865 diluting Methods 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 235000011187 glycerol Nutrition 0.000 description 4
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 230000003902 lesion Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000000600 sorbitol Substances 0.000 description 4
- 235000010356 sorbitol Nutrition 0.000 description 4
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 4
- 238000000108 ultra-filtration Methods 0.000 description 4
- QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 3
- 102100029516 Basic salivary proline-rich protein 1 Human genes 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 208000000907 Condylomata Acuminata Diseases 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 3
- 241000283074 Equus asinus Species 0.000 description 3
- 101000641177 Human papillomavirus type 16 Major capsid protein L1 Proteins 0.000 description 3
- 101100494726 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) pep-4 gene Proteins 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 230000010530 Virus Neutralization Effects 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 230000002457 bidirectional effect Effects 0.000 description 3
- 244000309466 calf Species 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- AGOYDEPGAOXOCK-KCBOHYOISA-N clarithromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@](C)([C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)OC)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 AGOYDEPGAOXOCK-KCBOHYOISA-N 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 210000004392 genitalia Anatomy 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 229910052744 lithium Inorganic materials 0.000 description 3
- 208000003154 papilloma Diseases 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 239000000825 pharmaceutical preparation Substances 0.000 description 3
- 230000009257 reactivity Effects 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000001338 self-assembly Methods 0.000 description 3
- 239000013605 shuttle vector Substances 0.000 description 3
- 238000007873 sieving Methods 0.000 description 3
- 239000012064 sodium phosphate buffer Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 229960004441 tyrosine Drugs 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 101710191936 70 kDa protein Proteins 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 2
- 241000450599 DNA viruses Species 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- 208000007842 Fibroepithelial Neoplasms Diseases 0.000 description 2
- 102100028501 Galanin peptides Human genes 0.000 description 2
- 101000803413 Human papillomavirus type 18 Minor capsid protein L2 Proteins 0.000 description 2
- 102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 2
- 101710184243 Intestinal-type alkaline phosphatase Proteins 0.000 description 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
- 229930182844 L-isoleucine Natural products 0.000 description 2
- 239000004395 L-leucine Substances 0.000 description 2
- 235000019454 L-leucine Nutrition 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 206010023849 Laryngeal papilloma Diseases 0.000 description 2
- 102000001109 Leukocyte L1 Antigen Complex Human genes 0.000 description 2
- 108010069316 Leukocyte L1 Antigen Complex Proteins 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 241000270295 Serpentes Species 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 229910021502 aluminium hydroxide Inorganic materials 0.000 description 2
- 210000002255 anal canal Anatomy 0.000 description 2
- 208000025009 anogenital human papillomavirus infection Diseases 0.000 description 2
- 201000004201 anogenital venereal wart Diseases 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 239000002518 antifoaming agent Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 208000012999 benign epithelial neoplasm Diseases 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 210000003679 cervix uteri Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000012501 chromatography medium Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000008121 dextrose Substances 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229910001679 gibbsite Inorganic materials 0.000 description 2
- 208000002672 hepatitis B Diseases 0.000 description 2
- 239000012510 hollow fiber Substances 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 201000004933 in situ carcinoma Diseases 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 208000020082 intraepithelial neoplasia Diseases 0.000 description 2
- 208000024312 invasive carcinoma Diseases 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- 201000000089 larynx squamous papilloma Diseases 0.000 description 2
- 230000003211 malignant effect Effects 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- YDSWCNNOKPMOTP-UHFFFAOYSA-N mellitic acid Chemical compound OC(=O)C1=C(C(O)=O)C(C(O)=O)=C(C(O)=O)C(C(O)=O)=C1C(O)=O YDSWCNNOKPMOTP-UHFFFAOYSA-N 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000013587 production medium Substances 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 210000005000 reproductive tract Anatomy 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000001384 succinic acid Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
- 239000012646 vaccine adjuvant Substances 0.000 description 2
- 229940124931 vaccine adjuvant Drugs 0.000 description 2
- 210000001215 vagina Anatomy 0.000 description 2
- 230000029812 viral genome replication Effects 0.000 description 2
- 210000003905 vulva Anatomy 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- NBTMNFYXJYCQHQ-UHFFFAOYSA-N (2,3,4,5,6-pentasulfooxycyclohexyl) hydrogen sulfate Chemical compound OS(=O)(=O)OC1C(OS(O)(=O)=O)C(OS(O)(=O)=O)C(OS(O)(=O)=O)C(OS(O)(=O)=O)C1OS(O)(=O)=O NBTMNFYXJYCQHQ-UHFFFAOYSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 1
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 1
- 240000000073 Achillea millefolium Species 0.000 description 1
- 235000007754 Achillea millefolium Nutrition 0.000 description 1
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- 241000701822 Bovine papillomavirus Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- FFEARJCKVFRZRR-SCSAIBSYSA-N D-methionine Chemical compound CSCC[C@@H](N)C(O)=O FFEARJCKVFRZRR-SCSAIBSYSA-N 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- 238000012286 ELISA Assay Methods 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 101150094690 GAL1 gene Proteins 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 101100121078 Homo sapiens GAL gene Proteins 0.000 description 1
- 101000641175 Human papillomavirus type 18 Major capsid protein L1 Proteins 0.000 description 1
- 241001428582 Human papillomavirus type 6 Species 0.000 description 1
- 108700025391 Human papillomavirus type 6 L1 Proteins 0.000 description 1
- 235000019766 L-Lysine Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 101710157639 Minor capsid protein Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- NTNWOCRCBQPEKQ-YFKPBYRVSA-N N(omega)-methyl-L-arginine Chemical compound CN=C(N)NCCC[C@H](N)C(O)=O NTNWOCRCBQPEKQ-YFKPBYRVSA-N 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 101710136297 Protein VP2 Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- MGSKVZWGBWPBTF-UHFFFAOYSA-N aebsf Chemical compound NCCC1=CC=C(S(F)(=O)=O)C=C1 MGSKVZWGBWPBTF-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 229940037003 alum Drugs 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 229940090047 auto-injector Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical class OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000006800 cellular catabolic process Effects 0.000 description 1
- 230000035606 childbirth Effects 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000000326 densiometry Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000011841 epidemiological investigation Methods 0.000 description 1
- 239000006167 equilibration buffer Substances 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 238000004374 forensic analysis Methods 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 235000020706 garlic extract Nutrition 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- JDNTWHVOXJZDSN-UHFFFAOYSA-N iodoacetic acid Chemical compound OC(=O)CI JDNTWHVOXJZDSN-UHFFFAOYSA-N 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- ADKOXSOCTOWDOP-UHFFFAOYSA-L magnesium;aluminum;dihydroxide;trihydrate Chemical compound O.O.O.[OH-].[OH-].[Mg+2].[Al] ADKOXSOCTOWDOP-UHFFFAOYSA-L 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- -1 nobility Proteins 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 235000020030 perry Nutrition 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 229960005190 phenylalanine Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 229940021993 prophylactic vaccine Drugs 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229940121649 protein inhibitor Drugs 0.000 description 1
- 239000012268 protein inhibitor Substances 0.000 description 1
- 229940114241 recombivax Drugs 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 210000001533 respiratory mucosa Anatomy 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012465 retentate Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000003998 size exclusion chromatography high performance liquid chromatography Methods 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229960002898 threonine Drugs 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Veterinary Medicine (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Dermatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24279494A | 1994-05-16 | 1994-05-16 | |
| PCT/US1995/006006 WO1995031532A1 (en) | 1994-05-16 | 1995-05-15 | Papillomavirus vaccines |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO964862D0 NO964862D0 (no) | 1996-11-15 |
| NO964862L NO964862L (no) | 1997-01-16 |
| NO322133B1 true NO322133B1 (no) | 2006-08-21 |
Family
ID=22916213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19964862A NO322133B1 (no) | 1994-05-16 | 1996-11-15 | Fremgangsmate for fremstilling av rensede viruslignende partikler (VLP-er) av humant papillomavirus. |
Country Status (24)
| Country | Link |
|---|---|
| EP (1) | EP0757717B1 (xx) |
| JP (1) | JP3863559B2 (xx) |
| CN (1) | CN1152935A (xx) |
| AT (1) | ATE328068T1 (xx) |
| AU (1) | AU693203B2 (xx) |
| BG (1) | BG100974A (xx) |
| BR (1) | BR9507657A (xx) |
| CA (1) | CA2189882C (xx) |
| CZ (1) | CZ336696A3 (xx) |
| DE (1) | DE69535018T2 (xx) |
| DK (1) | DK0757717T3 (xx) |
| ES (1) | ES2264126T3 (xx) |
| FI (1) | FI119815B (xx) |
| HU (1) | HUT76354A (xx) |
| MX (1) | MXPA96005662A (xx) |
| NO (1) | NO322133B1 (xx) |
| NZ (1) | NZ285941A (xx) |
| PL (1) | PL183781B1 (xx) |
| PT (1) | PT757717E (xx) |
| RO (1) | RO117541B1 (xx) |
| RU (1) | RU2206608C2 (xx) |
| SI (1) | SI0757717T1 (xx) |
| SK (1) | SK147696A3 (xx) |
| WO (1) | WO1995031532A1 (xx) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6153201A (en) * | 1993-03-09 | 2000-11-28 | University Of Rochester | Oral immunization with papillomavirus virus-like particles |
| PL319294A1 (en) * | 1994-09-22 | 1997-08-04 | Merck & Co Inc | Dna encoding the human papilloma virus of 6 a type |
| AR004464A1 (es) * | 1994-11-14 | 1998-12-16 | Merck Sharp & Dohme | Un metodo para producir una proteina de capside de papilomavirus |
| GB9621091D0 (en) | 1996-10-09 | 1996-11-27 | Fondation Pour Le Perfectionem | Attenuated microorganisms strains and their uses |
| SI1105466T1 (sl) | 1998-08-14 | 2006-08-31 | Merck & Co Inc | Postopek za ciscenje virusu podobnih delcev humanega papilomskega virusa |
| CA2773698C (en) | 1998-10-16 | 2015-05-19 | Glaxosmithkline Biologicals S.A. | Adjuvant systems comprising an immunostimulant adsorbed to a metallic salt particle and vaccines thereof |
| AUPP765398A0 (en) * | 1998-12-11 | 1999-01-14 | University Of Queensland, The | Treatment of papillomavirus infections |
| SI1150712T1 (sl) * | 1999-02-05 | 2009-02-28 | Merck & Co Inc | Pripravek cepiva proti humanem papiloma virusu |
| DE10137102A1 (de) | 2001-07-30 | 2003-02-27 | Deutsches Krebsforsch | Polyvalente Vakzine gegen durch Papillomaviren verursachte Erkrankungen, Verfahren zu deren Herstellung und deren Verwendung |
| GB0206360D0 (en) * | 2002-03-18 | 2002-05-01 | Glaxosmithkline Biolog Sa | Viral antigens |
| WO2003093463A1 (en) | 2002-04-30 | 2003-11-13 | Oncolytics Biotech Inc. | Improved viral purification methods |
| US7858098B2 (en) | 2002-12-20 | 2010-12-28 | Glaxosmithkline Biologicals, S.A. | Vaccine |
| MY140664A (en) * | 2003-09-29 | 2010-01-15 | Merck Sharp & Dohme | Optimized expression of hpv 45 l1 in yeast |
| EP2550971B1 (en) * | 2004-09-30 | 2016-11-09 | Bayer HealthCare LLC | Devices and Methods for Integrated Continuous Manufacturing of Biological Molecules |
| US7901921B2 (en) | 2004-10-22 | 2011-03-08 | Oncolytics Biotech Inc. | Viral purification methods |
| SG174845A1 (en) | 2006-09-29 | 2011-10-28 | Ligocyte Pharmaceuticals Inc | Norovirus vaccine formulations |
| AU2008302276B2 (en) | 2006-09-29 | 2013-10-10 | Takeda Vaccines, Inc. | Method of conferring a protective immune response to norovirus |
| PE20081723A1 (es) * | 2007-01-30 | 2008-12-14 | Transgene Sa | Vacuna contra el papilomavirus |
| KR20150098681A (ko) | 2007-03-14 | 2015-08-28 | 다케다 백신즈 인코포레이티드 | 바이러스 유사 입자 정제 |
| NZ582271A (en) | 2007-06-26 | 2012-03-30 | Japan Health Science Found | Vaccine antigen capable of inducing cross-reacting and neutralizing antibody against high-risk-type human papillomavirus |
| US20100266636A1 (en) | 2007-09-18 | 2010-10-21 | Ligocyte Pharmaceuticals, Inc. | Method of conferring a protective immune response to norovirus |
| EP2223933B1 (en) * | 2007-11-23 | 2016-01-27 | Shanghai Zerun Biotechnology Co., Ltd. | Genes encoding major capsid protein l1 of human papilloma viruses |
| DK2324113T3 (en) | 2008-08-08 | 2018-04-16 | Takeda Vaccines Inc | VIRUS-LIKE PARTICLES INCLUDING COMPOSITE CAPSID AMINO ACID SEQUENCES FOR INCREASED CRYSTAL REACTIVITY |
| WO2010147268A1 (ko) * | 2009-06-19 | 2010-12-23 | 아이진 주식회사 | 자궁경부암 백신 |
| CN102234661A (zh) * | 2010-04-23 | 2011-11-09 | 北京生物制品研究所 | 人乳头瘤病毒类病毒颗粒的制备方法和外源蛋白表达盒、表达系统 |
| DK2646052T3 (en) | 2010-12-02 | 2017-07-17 | Oncolytics Biotech Inc | LYOPHILIZED VIRAL FORMULATIONS |
| TW201233802A (en) | 2010-12-02 | 2012-08-16 | Oncolytics Biotech Inc | Liquid viral formulations |
| RU2445357C1 (ru) * | 2011-02-15 | 2012-03-20 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | Рекомбинантный штамм дрожжей pichia angusta - продуцент капсидного белка l1 вируса папилломы человека типа 16 |
| RU2445358C1 (ru) * | 2011-02-15 | 2012-03-20 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | Рекомбинантный штамм дрожжей pichia angusta - продуцент капсидного белка l1 вируса папилломы человека типа 18 |
| DK2702147T3 (da) * | 2011-04-29 | 2020-09-28 | Oncolytics Biotech Inc | Fremgangsmåder til oprensning af vira ved hjælp af gelpermeationskromatografi |
| WO2013009849A1 (en) | 2011-07-11 | 2013-01-17 | Ligocyte Pharmaceuticals, Inc. | Parenteral norovirus vaccine formulations |
| JP6228922B2 (ja) * | 2011-12-01 | 2017-11-08 | ユニバーシティ・オブ・ケープ・タウンＵｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｐｅ Ｔｏｗｎ | Ｈｐｖキメラ粒子 |
| CN103215302B (zh) * | 2012-01-21 | 2019-01-15 | 北京安百胜生物科技有限公司 | 用汉逊酵母表达系统产生hpv18 l1蛋白的方法 |
| CN103361377B (zh) * | 2012-03-28 | 2017-12-05 | 北京安百胜生物科技有限公司 | 用汉逊酵母表达系统产生hpv6 l1蛋白的方法 |
| JOP20130186B1 (ar) | 2012-06-22 | 2021-08-17 | Takeda Vaccines Montana Inc | تنقية الجزيئات الشبيهة بالفيروسات |
| CN104120089B (zh) * | 2013-04-26 | 2019-09-24 | 北京安百胜生物科技有限公司 | 用汉逊酵母表达系统产生hpv52 l1蛋白的方法 |
| CN104120088B (zh) * | 2013-04-26 | 2019-09-24 | 北京安百胜生物科技有限公司 | 用汉逊酵母表达系统产生hpv58 l1蛋白的方法 |
| CN110592133A (zh) * | 2013-05-17 | 2019-12-20 | 北京安百胜生物科技有限公司 | 用汉逊酵母表达系统产生hpv31 l1蛋白的方法 |
| CN104673760B (zh) * | 2013-11-29 | 2018-01-02 | 南京赛威信生物医药有限公司 | 一种原核细胞表达类病毒颗粒的纯化方法 |
| RU2546240C1 (ru) * | 2014-02-13 | 2015-04-10 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | РЕКОМБИНАНТНЫЙ ШТАММ ДРОЖЖЕЙ Hansenula polymorpha - ПРОДУЦЕНТ ГЛАВНОГО КАПСИДНОГО БЕЛКА L1 ВИРУСА ПАПИЛЛОМЫ ЧЕЛОВЕКА ТИПА 56 |
| RU2546241C1 (ru) * | 2014-02-13 | 2015-04-10 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | РЕКОМБИНАНТНЫЙ ШТАММ ДРОЖЖЕЙ Hansenula polymorpha - ПРОДУЦЕНТ ГЛАВНОГО КАПСИДНОГО БЕЛКА L1 ВИРУСА ПАПИЛЛОМЫ ЧЕЛОВЕКА ТИПА 16 |
| RU2546243C1 (ru) * | 2014-02-13 | 2015-04-10 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | Рекомбинантная вакцина для профилактики папилломавирусной инфекции человека и способ ее получения |
| RU2546242C1 (ru) * | 2014-02-13 | 2015-04-10 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | РЕКОМБИНАНТНЫЙ ШТАММ ДРОЖЖЕЙ Hansenula polymorpha - ПРОДУЦЕНТ ГЛАВНОГО КАПСИДНОГО БЕЛКА L1 ВИРУСА ПАПИЛЛОМЫ ЧЕЛОВЕКА ТИПА 18 |
| RU2677336C2 (ru) * | 2014-12-26 | 2019-01-16 | Айджин, Инк. | Способ получения вирусоподобных частиц папилломавируса человека |
| EP3344293A4 (en) * | 2015-09-04 | 2019-01-16 | Inventprise, LLC. | STABILIZED VACCINE COMPOSITIONS OF VLP |
| RU2676160C1 (ru) * | 2018-02-14 | 2018-12-26 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | Рекомбинантный штамм дрожжей Hansenula polymorpha - продуцент главного капсидного белка L1 вируса папилломы человека типа 11 |
| RU2681174C1 (ru) * | 2018-07-12 | 2019-03-04 | Закрытое акционерное общество научно-производственная компания "Комбиотех" | Способ получения рекомбинантной вакцины для профилактики папилломавирусной инфекции человека, рекомбинантная вакцина |
| CN116426396B (zh) * | 2023-06-09 | 2023-09-01 | 内蒙古工业大学 | 一株耐硫酸铵酿酒酵母及其筛选方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2586428B1 (fr) * | 1985-08-26 | 1988-11-25 | Pasteur Institut | Polypeptides et anticorps, caracteristiques du papillomavirus et leurs applications au diagnostic in vitro, a la prevention et/ou la lutte contre des infections a papillomavirus |
| DE3625257A1 (de) * | 1986-07-23 | 1988-02-04 | Behringwerke Ag | Expressionsprodukte der menschlichen papillomviren typ 16 und 18, fuer diese proteine spezifische antikoerper und diese antikoerper bzw. entsprechende dna enthaltende diagnostika |
| US7476389B1 (en) * | 1991-07-19 | 2009-01-13 | The University Of Queensland | Papillomavirus vaccines |
| DE122007000095I1 (de) * | 1992-06-25 | 2008-03-27 | Papillomavirus vakzine | |
| US5437951A (en) * | 1992-09-03 | 1995-08-01 | The United States Of America As Represented By The Department Of Health And Human Services | Self-assembling recombinant papillomavirus capsid proteins |
| DE4332596A1 (de) * | 1993-09-24 | 1995-03-30 | Martin Josef Dr Sapp | Monoklonale Antikörper |
| AUPM566794A0 (en) * | 1994-05-17 | 1994-06-09 | University Of Queensland, The | Process and product |
-
1995
- 1995-05-15 CN CN95194158A patent/CN1152935A/zh active Pending
- 1995-05-15 MX MXPA96005662A patent/MXPA96005662A/es active IP Right Grant
- 1995-05-15 SK SK1476-96A patent/SK147696A3/sk unknown
- 1995-05-15 AT AT95919820T patent/ATE328068T1/de active
- 1995-05-15 EP EP95919820A patent/EP0757717B1/en not_active Expired - Lifetime
- 1995-05-15 BR BR9507657A patent/BR9507657A/pt not_active Application Discontinuation
- 1995-05-15 PT PT95919820T patent/PT757717E/pt unknown
- 1995-05-15 DK DK95919820T patent/DK0757717T3/da active
- 1995-05-15 SI SI9530726T patent/SI0757717T1/sl unknown
- 1995-05-15 DE DE69535018T patent/DE69535018T2/de not_active Expired - Lifetime
- 1995-05-15 WO PCT/US1995/006006 patent/WO1995031532A1/en active IP Right Grant
- 1995-05-15 PL PL95317234A patent/PL183781B1/pl unknown
- 1995-05-15 JP JP52980895A patent/JP3863559B2/ja not_active Expired - Lifetime
- 1995-05-15 AU AU25495/95A patent/AU693203B2/en not_active Expired
- 1995-05-15 HU HU9603155A patent/HUT76354A/hu unknown
- 1995-05-15 CA CA002189882A patent/CA2189882C/en not_active Expired - Lifetime
- 1995-05-15 ES ES95919820T patent/ES2264126T3/es not_active Expired - Lifetime
- 1995-05-15 RU RU96123707/13A patent/RU2206608C2/ru active
- 1995-05-15 CZ CZ963366A patent/CZ336696A3/cs unknown
- 1995-05-15 RO RO96-02165A patent/RO117541B1/ro unknown
- 1995-06-02 NZ NZ285941A patent/NZ285941A/en not_active IP Right Cessation
-
1996
- 1996-11-12 BG BG100974A patent/BG100974A/xx unknown
- 1996-11-15 NO NO19964862A patent/NO322133B1/no not_active IP Right Cessation
- 1996-11-15 FI FI964592A patent/FI119815B/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI119815B (fi) | 2009-03-31 |
| EP0757717B1 (en) | 2006-05-31 |
| CZ336696A3 (en) | 1997-07-16 |
| DE69535018D1 (de) | 2006-07-06 |
| EP0757717A1 (en) | 1997-02-12 |
| PT757717E (pt) | 2006-09-29 |
| JPH10500847A (ja) | 1998-01-27 |
| BG100974A (en) | 1998-04-30 |
| AU2549595A (en) | 1995-12-05 |
| BR9507657A (pt) | 1997-09-09 |
| NO964862L (no) | 1997-01-16 |
| EP0757717A4 (en) | 1998-04-15 |
| MXPA96005662A (es) | 2004-08-19 |
| NZ285941A (en) | 1998-05-27 |
| DK0757717T3 (da) | 2006-09-25 |
| RO117541B1 (ro) | 2002-04-30 |
| WO1995031532A1 (en) | 1995-11-23 |
| DE69535018T2 (de) | 2007-02-15 |
| HU9603155D0 (en) | 1997-01-28 |
| ES2264126T3 (es) | 2006-12-16 |
| CA2189882C (en) | 2005-09-20 |
| RU2206608C2 (ru) | 2003-06-20 |
| HUT76354A (en) | 1997-08-28 |
| CA2189882A1 (en) | 1995-11-23 |
| PL183781B1 (pl) | 2002-07-31 |
| CN1152935A (zh) | 1997-06-25 |
| FI964592A (fi) | 1996-11-15 |
| PL317234A1 (en) | 1997-03-17 |
| SI0757717T1 (sl) | 2006-08-31 |
| NO964862D0 (no) | 1996-11-15 |
| FI964592A0 (fi) | 1996-11-15 |
| SK147696A3 (en) | 1997-08-06 |
| ATE328068T1 (de) | 2006-06-15 |
| JP3863559B2 (ja) | 2006-12-27 |
| AU693203B2 (en) | 1998-06-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0757717B1 (en) | Papillomavirus vaccines | |
| JP3918877B2 (ja) | ヒトパピローマウイルス１１型ｌ１タンパク質をコードする合成ｈｐｖ６／１１ハイブリッドｌ１ ｄｎａ | |
| US5821087A (en) | Production of recombinant human papillomavirus type II protein utilizing papillomavirus 6/11 hybrid DNA | |
| EP0789767B1 (en) | A method for producing purified papillomavirus proteins | |
| EP0973546B1 (en) | Stabilized human papillomavirus formulations | |
| EP0817851B1 (en) | Dna encoding human papilloma virus type 18 | |
| US5888516A (en) | Recombinant papillomavirus vaccines | |
| WO1996015247A9 (en) | Purified papillomavirus proteins | |
| CA2204266C (en) | Purified papillomavirus proteins | |
| CA2216827C (en) | Synthetic hpv6/11 hybrid l1 dna encoding human papillomavirus type 11 l1 protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK1K | Patent expired |