NO20091371L - Prosess for kromosomintegrasjon og DNA-sekvensutskiftning i Clostridia - Google Patents

Prosess for kromosomintegrasjon og DNA-sekvensutskiftning i Clostridia

Info

Publication number
NO20091371L
NO20091371L NO20091371A NO20091371A NO20091371L NO 20091371 L NO20091371 L NO 20091371L NO 20091371 A NO20091371 A NO 20091371A NO 20091371 A NO20091371 A NO 20091371A NO 20091371 L NO20091371 L NO 20091371L
Authority
NO
Norway
Prior art keywords
clostridia
dna sequence
sequence replacement
chromosome integration
chromosome
Prior art date
Application number
NO20091371A
Other languages
English (en)
Inventor
Philippe Soucaille
Rainer Figge
Christian Croux
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37605677&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NO20091371(L) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of NO20091371L publication Critical patent/NO20091371L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Foreliggende oppfinnelse angår en ny fremgangsmåte for å skifte u teller fjerne DNA-sekvenser i Clostridia, med høy effektivitet, enkel å utføre og anvendbar på et industrielt nivå. Denne fremgangsmåten er nyttig til å modifisere flere genetiske steder i Clostridia på en rutinemessig måte. Denne fremgangsmåten er basert på en replikasjonsvektor som bærer minst to markørgener.
NO20091371A 2006-10-03 2009-04-03 Prosess for kromosomintegrasjon og DNA-sekvensutskiftning i Clostridia NO20091371L (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2006/066997 WO2008040387A1 (en) 2006-10-03 2006-10-03 Process for chromosomal integration and dna sequence replacement in clostridia

Publications (1)

Publication Number Publication Date
NO20091371L true NO20091371L (no) 2009-04-03

Family

ID=37605677

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20091371A NO20091371L (no) 2006-10-03 2009-04-03 Prosess for kromosomintegrasjon og DNA-sekvensutskiftning i Clostridia

Country Status (18)

Country Link
US (2) US8168434B2 (no)
EP (2) EP2316952A1 (no)
JP (1) JP5203375B2 (no)
KR (1) KR101311619B1 (no)
CN (1) CN101528930B (no)
AR (1) AR063126A1 (no)
AT (1) ATE517995T1 (no)
AU (1) AU2006349179A1 (no)
BR (1) BRPI0622182B1 (no)
CA (1) CA2665120A1 (no)
CL (1) CL2007002857A1 (no)
DK (1) DK1908840T3 (no)
ES (1) ES2369859T3 (no)
IL (1) IL197844A0 (no)
MX (1) MX2009003605A (no)
NO (1) NO20091371L (no)
SI (1) SI1908840T1 (no)
WO (1) WO2008040387A1 (no)

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US7432090B2 (en) * 2004-07-01 2008-10-07 Rice University Blocking sporulation by inhibiting SpoIIE
EP2084288A1 (en) 2006-10-31 2009-08-05 Metabolic Explorer Process for the biological production of 1,3-propanediol from glycerol with high yield
EP2192177A1 (en) 2008-11-28 2010-06-02 Total S.A. Cellulase Cel5H related reagents and their use in microorganisms
WO2010022763A1 (en) * 2008-08-25 2010-03-04 Metabolic Explorer Method for the preparation of 2-hydroxy-isobutyrate
EP2194120A1 (en) 2008-12-02 2010-06-09 Total S.A. Bioprocessing ligno-cellulose into ethanol with recombinant clostridium
ES2549003T3 (es) 2008-12-31 2015-10-22 Metabolic Explorer Método para la preparación de dioles
GB0901001D0 (en) * 2009-01-22 2009-03-04 Univ Nottingham Methods
EP2267141A1 (en) * 2009-06-26 2010-12-29 Metabolic Explorer Process for the biological production of n-Butanol with high yield
EP2267126A1 (en) * 2009-06-26 2010-12-29 Metabolic Explorer Process for the stable gene interruption in clostridia
KR101647143B1 (ko) * 2009-09-22 2016-08-10 지에스칼텍스 주식회사 클로스트리듐 속 미생물 유전자를 불활성화시키는 방법
US20110117655A1 (en) * 2009-11-18 2011-05-19 Bryan Tracy Methods and compositions for genetically manipulating clostridia and related bacteria with homologous recombination associated proteins
US8911978B2 (en) 2010-07-02 2014-12-16 Metabolic Explorer Method for the preparation of hydroxy acids
TWI500768B (zh) 2010-07-05 2015-09-21 Metabolic Explorer Sa 由蔗糖製備１，３－丙二醇之方法
US20130295631A1 (en) 2010-10-01 2013-11-07 The Board Of Trustees Of The University Of Illinois Combinatorial design of highly efficient heterologous pathways
AR083799A1 (es) 2010-11-10 2013-03-20 Metabolic Explorer Sa Microorganismos para la produccion de 1,3-propanodiol usando concentracion de glicerina alta
SG190321A1 (en) 2010-11-24 2013-06-28 Novartis Int Pharm Ltd Fusion enzymes having n-acetylglucosaminyltransferase activity
CN102559704B (zh) * 2010-12-23 2014-08-27 中国科学院上海生命科学研究院 一种在丙酮丁醇梭菌中敲除基因的方法
WO2012100196A1 (en) 2011-01-21 2012-07-26 The Board Of Trustees Of The University Of Illinois ENHANCED FERMENTATION OF CELLODEXTRINS AND β-D-GLUCOSE
CA2826570A1 (en) 2011-02-07 2012-08-16 The Regents Of The University Of California Enhanced cellodextrin metabolism
US8692024B2 (en) 2011-02-14 2014-04-08 Eastman Renewable Materials, Llc Method of producing n-butyraldehyde
US9267106B2 (en) * 2011-02-14 2016-02-23 Eastman Renewable Materials, Llc Method for incorporation of recombinant DNA
EP2540834A1 (en) 2011-06-29 2013-01-02 Metabolic Explorer Method for the preparation of 1,3-propanediol
EP2766492B1 (en) 2011-10-11 2018-06-06 Metabolic Explorer Fermentative production of prenol
WO2013059326A1 (en) 2011-10-17 2013-04-25 The Board Of Trustees Of The University Of Illinois Xylitol production from cellulosic biomass
FR2982276A1 (fr) 2011-11-08 2013-05-10 Univ Grenoble 1 Cassette d'expression pour modifier le genome des bacteries et pour modifier un vecteur dans une souche bacterienne
CN103125447B (zh) * 2011-11-24 2014-10-15 上海西普尔-必凯实验动物有限公司 一种1号染色体替换实验小鼠品系C57BL/6-Chr1C3H/HeJ的构建
WO2013133882A2 (en) * 2011-12-16 2013-09-12 University Of Delaware Recombinant clostridium organism and method for isolation of double-crossover allelic exchange mutants
EP2820134A1 (en) 2012-03-02 2015-01-07 Metabolic Explorer A process for butanol production
EP2647718A3 (en) 2012-04-06 2014-12-24 Metabolic Explorer Process for producing 5-aminopentanoate empolying a recombinant microorganism
WO2013155481A1 (en) 2012-04-13 2013-10-17 The Board Of Trustees Of The University Of Illinois Improved cellodextrin transport and mixed sugar fermentation
WO2014049382A2 (en) 2012-09-26 2014-04-03 Metabolic Explorer Ethylenediamine fermentative production by a recombinant microorganism
CN103719021B (zh) * 2012-10-10 2017-06-20 上海西普尔-必凯实验动物有限公司 1号染色体替换野生小家鼠品系C57BL/6.枣庄2‑Chr1的构建
US9745566B2 (en) * 2013-09-12 2017-08-29 Lanzatech New Zealand Limited Recombinant microorganisms and methods of use thereof
GB201406970D0 (en) * 2014-04-17 2014-06-04 Green Biologics Ltd Targeted mutations
GB201406968D0 (en) * 2014-04-17 2014-06-04 Green Biologics Ltd Deletion mutants
WO2017017026A1 (en) 2015-07-27 2017-02-02 Institut National Des Sciences Appliquees New polypeptide having ferredoxin-nadp+ reductase activity, polynucleotide encoding the same and uses thereof
WO2017068385A1 (en) 2015-10-23 2017-04-27 Metabolic Explorer Microorganism modified for the assimilation of levulinic acid
CN110325645A (zh) 2017-02-20 2019-10-11 代谢探索者公司 用于使用高丙三醇浓度产生1,3-丙二醇的微生物聚生体
EP3438270A1 (en) 2017-08-04 2019-02-06 Metabolic Explorer Microorganism and method for improved 1,3-propanediol production by fermentation on a culture medium with high glycerine content
MX2021001619A (es) 2018-08-10 2021-05-12 Metabolic Explorer Sa Microorganismos con produccion mejorada de 1,3-propanodiol y acido butirico.
FR3111642A1 (fr) 2020-06-23 2021-12-24 IFP Energies Nouvelles Souches de bacteriesclostridiumresistantes au 5-fluorouracile, outils genetiques et utilisations de ceux-ci
WO2024056898A1 (en) 2022-09-15 2024-03-21 Institut National Des Sciences Appliquees De Toulouse Genetically modified lignocellulolytic clostridium acetobutylicum

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US6673567B2 (en) * 2000-03-23 2004-01-06 E. I. Du Pont De Nemours And Company Method of determination of gene function
GB0008682D0 (en) 2000-04-07 2000-05-31 Microbiological Res Authority Transformation of clostridium difficile
GB0414832D0 (en) * 2004-07-01 2004-08-04 Cobra Biolog Ltd Process for the removal of selectable marker gene sequences

Also Published As

Publication number Publication date
US20120190116A1 (en) 2012-07-26
BRPI0622182A2 (pt) 2011-12-27
BRPI0622182B1 (pt) 2018-07-31
EP1908840B1 (en) 2011-07-27
ATE517995T1 (de) 2011-08-15
EP1908840A1 (en) 2008-04-09
CL2007002857A1 (es) 2008-04-18
ES2369859T3 (es) 2011-12-07
AR063126A1 (es) 2008-12-30
IL197844A0 (en) 2011-08-01
KR101311619B1 (ko) 2013-09-26
EP1908840A8 (en) 2008-06-25
CA2665120A1 (en) 2008-04-10
MX2009003605A (es) 2009-04-22
US8628967B2 (en) 2014-01-14
US20080085558A1 (en) 2008-04-10
SI1908840T1 (sl) 2011-10-28
JP2010505398A (ja) 2010-02-25
WO2008040387A1 (en) 2008-04-10
US8168434B2 (en) 2012-05-01
JP5203375B2 (ja) 2013-06-05
CN101528930A (zh) 2009-09-09
KR20090086939A (ko) 2009-08-14
DK1908840T3 (da) 2011-09-19
EP2316952A1 (en) 2011-05-04
AU2006349179A1 (en) 2008-04-10
CN101528930B (zh) 2013-12-11

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